AT5G63040

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009561
Physical Location & Seq
Forward (+)
25288146 .. 25290420
2275 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G63040.2

Sequence Viewer

Length: 1101 bp
ATGGCTGCTAAACTTGTGTCCTCCTCGGCTTCTTCTTTGGTTCCATCCATTTTCTCGCCGTTTCAACCTCGGAGTTCCTTTCAGTTTCGTTCACCGAGGGTTCAGCCTGCTCATCTCCATTGTTACCATTCTCTGCACCTTAAAACTGATAAAATTGGCTCTCTTCACATTTCCTCAAGAGGCCAAAAACCAAGCGAAGTCTCCCGAAAGAGAACTTATTTGCCTCTTGCTACTTCAGAAGAAAAGTTCCACTACACTGAAGATACAACAAATGATCCAGACACAGTAAGTCCCCAAATTGGCACCGAAGCTACACCTAGAGATGATGATTCAACTATCCAATACAATAGAAATGACGGGAAACCGGGTTTCATTTCGTTTTACAATCCTCGGAATAAAACTGAGGATATCATTATCCCCCCTGAAACTCAAAGTCCATGGGGACGCCTGCTCTGGCTCATTGGTCCTGCAGTCTTAGTCTCCTCTTTCATTTTACCTCCAGTTTACTTACGAAGAATAGTCTCAGCAGTTTTTGAAGATTCTTTGCTCACAGACTTCCTCATCCTGTTCTTCACCGAGGCTCTCTTCTACTGCGGAGTCGCTGCATTTCTTCTGATAATAGACCGCTCAAGAAAAGGATCCGGAAAAGTTCCCCAGAACCGAATCAATCCTTCTCAGTTAGGACAAAGAATCTCCTCTGTAGCAACATTAGTGCTGAGTCTCATGATTCCAATGGTGACAATGGGATTCGTCTGGCCATGGACTGGACCTGCAGCATCAGCTACTCTTGCACCATACCTCGTTGGTATCGTTGTTCAGTTTGCATTCGAACAGTACGCAAGATATCGTAACTCTCCATCATCTCCTATCATCCCCATCATCTTTCAGGTATACAGACTTCATCAGCTGAACCGAGCGGCGCAACTAGTAACAGCATTGTCATTTACGGTTAAAGGAGCAGAGGCAACAGTTAATAATCTGGCAATCAAGAAATCACTGGGCACACTTTTGAATGTGATACAGGTTTTGGGTGTGATTTCAATCTGGTCTATATCTAGCTTCCTCATGTGGTTATCATCACCTTCCCAAAACCAGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

366

Amino Acids

40.66

Weight (kDa)

9.64

Isoelectric Point (pI)

50.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 778
AccB1I GGYRCC 1 cut(s) 302
AccB7I CCANNNNNTGG 1 cut(s) 764
AccBSI CCGCTC 2 cut(s) 627, 917
AccI GTMKAC 1 cut(s) 891
AccIII TCCGGA 1 cut(s) 641
AciI CCGC 3 cut(s) 594, 625, 917
AclWI GGATC 3 cut(s) 269, 633, 646
AcoI YGGCCR 1 cut(s) 755
AcuI CTGAAG 2 cut(s) 219, 279
AcyI GRCGYC 1 cut(s) 445
AfaI GTAC 1 cut(s) 836
AfiI CCNNNNNNNGG 2 cut(s) 299, 764
AgsI TTSAA 5 cut(s) 65, 333, 536, 1012, 1041
AhlI ACTAGT 1 cut(s) 925
AluBI AGCT 4 cut(s) 311, 782, 907, 1059
AluI AGCT 4 cut(s) 311, 782, 907, 1059
Alw26I GTCTC 4 cut(s) 205, 484, 526, 725
AlwI GGATC 3 cut(s) 269, 633, 646
Aor13HI TCCGGA 1 cut(s) 641
AoxI GGCC 2 cut(s) 181, 755
ApeKI GCWGC 3 cut(s) 5, 602, 773
AspLEI GCGC 1 cut(s) 922
AspS9I GGNCC 2 cut(s) 464, 767
AsuC2I CCSGG 1 cut(s) 366
AsuHPI GGTGA 4 cut(s) 84, 565, 748, 1071
AsuII TTCGAA 1 cut(s) 828
AvaII GGWCC 2 cut(s) 464, 767
BaeGI GKGCMC 1 cut(s) 1004
BalI TGGCCA 1 cut(s) 757
BamHI GGATCC 1 cut(s) 638
BanI GGYRCC 1 cut(s) 302
BarI GAAGNNNNNNTAC 2 cut(s) 882, 914
BbvI GCAGC 2 cut(s) 589, 785
BccI CCATC 3 cut(s) 52, 865, 884
BceAI ACGGC 1 cut(s) 43
BcnI CCSGG 1 cut(s) 366
BcoDI GTCTC 4 cut(s) 205, 484, 526, 725
BcuI ACTAGT 1 cut(s) 925
BfaI CTAG 3 cut(s) 318, 926, 1056
BfmI CTRYAG 3 cut(s) 468, 699, 771
BfuAI ACCTGC 1 cut(s) 778
BisI GCNGC 4 cut(s) 6, 603, 774, 918
BlsI GCNGC 4 cut(s) 7, 604, 775, 919
Bme1390I CCNGG 1 cut(s) 366
Bme18I GGWCC 2 cut(s) 464, 767
BmgT120I GGNCC 2 cut(s) 464, 767
BmiI GGNNCC 3 cut(s) 42, 304, 640
BmrFI CCNGG 1 cut(s) 366
BmrI ACTGGG 1 cut(s) 1007
BmsI GCATC 1 cut(s) 785
BmuI ACTGGG 1 cut(s) 1007
BpmI CTGGAG 1 cut(s) 483
Bpu14I TTCGAA 1 cut(s) 828
BpuEI CTTGAG 2 cut(s) 160, 613
BpuMI CCSGG 1 cut(s) 366
BsaBI GATNNNNATC 1 cut(s) 1040
BsaHI GRCGYC 1 cut(s) 445
BsaJI CCNNGG 7 cut(s) 24, 68, 95, 389, 437, 576, 758
BsaWI WCCGGW 1 cut(s) 641
Bsc4I CCNNNNNNNGG 2 cut(s) 299, 764
Bse1I ACTGG 4 cut(s) 500, 769, 1002, 1093
Bse8I GATNNNNATC 1 cut(s) 1040
BseAI TCCGGA 1 cut(s) 641
BseDI CCNNGG 7 cut(s) 24, 68, 95, 389, 437, 576, 758
BseGI GGATG 3 cut(s) 44, 561, 870
BseJI GATNNNNATC 1 cut(s) 1040
BseLI CCNNNNNNNGG 2 cut(s) 299, 764
BseMII CTCAG 4 cut(s) 393, 537, 689, 707
BseNI ACTGG 4 cut(s) 500, 769, 1002, 1093
BseRI GAGGAG 3 cut(s) 13, 472, 685
BseSI GKGCMC 1 cut(s) 1004
BseXI GCAGC 2 cut(s) 589, 785
BsgI GTGCAG 1 cut(s) 119
BshFI GGCC 2 cut(s) 183, 757
BshNI GGYRCC 1 cut(s) 302
BsiSI CCGG 2 cut(s) 365, 642
BslFI GGGAC 2 cut(s) 276, 456
BslI CCNNNNNNNGG 2 cut(s) 299, 764
BsmAI GTCTC 4 cut(s) 205, 484, 526, 725
BsmFI GGGAC 2 cut(s) 276, 456
BsmI GAATGC 1 cut(s) 824
BsnI GGCC 2 cut(s) 183, 757
Bsp119I TTCGAA 1 cut(s) 828
Bsp1286I GDGCHC 1 cut(s) 1004
Bsp13I TCCGGA 1 cut(s) 641
Bsp143I GATC 2 cut(s) 274, 638
Bsp19I CCATGG 2 cut(s) 437, 758
BspACI CCGC 3 cut(s) 594, 625, 917
BspANI GGCC 2 cut(s) 183, 757
BspCNI CTCAG 4 cut(s) 394, 536, 688, 708
BspEI TCCGGA 1 cut(s) 641
BspHI TCATGA 1 cut(s) 723
BspLI GGNNCC 3 cut(s) 42, 304, 640
BspMAI CTGCAG 2 cut(s) 472, 775
BspMI ACCTGC 1 cut(s) 778
BspPI GGATC 3 cut(s) 269, 633, 646
BspT104I TTCGAA 1 cut(s) 828
BspT107I GGYRCC 1 cut(s) 302
BsrBI CCGCTC 2 cut(s) 627, 917
BsrI ACTGG 4 cut(s) 500, 769, 1002, 1093
BssECI CCNNGG 7 cut(s) 24, 68, 95, 389, 437, 576, 758
BssMI GATC 2 cut(s) 274, 638
BssNAI GTATAC 1 cut(s) 892
BssNI GRCGYC 1 cut(s) 445
BssT1I CCWWGG 2 cut(s) 437, 758
Bst1107I GTATAC 1 cut(s) 892
Bst4CI ACNGT 4 cut(s) 286, 834, 949, 970
Bst6I CTCTTC 2 cut(s) 168, 590
BstACI GRCGYC 1 cut(s) 445
BstBI TTCGAA 1 cut(s) 828
BstC8I GCNNGC 2 cut(s) 108, 449
BstDEI CTNAG 5 cut(s) 402, 475, 523, 675, 716
BstDSI CCRYGG 2 cut(s) 437, 758
BstF5I GGATG 3 cut(s) 44, 561, 870
BstHHI GCGC 1 cut(s) 922
BstKTI GATC 2 cut(s) 277, 641
BstMAI GTCTC 4 cut(s) 205, 484, 526, 725
BstMBI GATC 2 cut(s) 274, 638
BstMWI GCNNNNNNNGC 2 cut(s) 779, 788
BstSCI CCNGG 1 cut(s) 364
BstSFI CTRYAG 3 cut(s) 468, 699, 771
BstSLI GKGCMC 1 cut(s) 1004
BstV1I GCAGC 2 cut(s) 589, 785
BstX2I RGATCY 1 cut(s) 638
BstYI RGATCY 1 cut(s) 638
BstZ17I GTATAC 1 cut(s) 892
BsuRI GGCC 2 cut(s) 183, 757
BtgI CCRYGG 2 cut(s) 437, 758
BtsCI GGATG 3 cut(s) 44, 561, 870
BtsIMutI CAGTG 2 cut(s) 255, 995
BveI ACCTGC 1 cut(s) 778
Cac8I GCNNGC 2 cut(s) 108, 449
CciI TCATGA 1 cut(s) 723
CfoI GCGC 1 cut(s) 922
Cfr13I GGNCC 2 cut(s) 464, 767
CseI GACGC 1 cut(s) 453
Csp6I GTAC 1 cut(s) 835
CviAII CATG 4 cut(s) 438, 724, 759, 1066
CviQI GTAC 1 cut(s) 835
DdeI CTNAG 5 cut(s) 402, 475, 523, 675, 716
DpnI GATC 2 cut(s) 276, 640
DpnII GATC 2 cut(s) 274, 638
EaeI YGGCCR 1 cut(s) 755
Eam1104I CTCTTC 2 cut(s) 168, 590
EarI CTCTTC 2 cut(s) 168, 590
Eco130I CCWWGG 2 cut(s) 437, 758
Eco32I GATATC 2 cut(s) 409, 845
Eco47I GGWCC 2 cut(s) 464, 767
Eco57I CTGAAG 2 cut(s) 219, 279
EcoRV GATATC 2 cut(s) 409, 845
EcoT14I CCWWGG 2 cut(s) 437, 758
ErhI CCWWGG 2 cut(s) 437, 758
FaeI CATG 4 cut(s) 441, 727, 762, 1069
FaiI YATR 7 cut(s) 439, 725, 760, 796, 892, 1052, 1067
FaqI GGGAC 2 cut(s) 276, 456
FatI CATG 4 cut(s) 437, 723, 758, 1065
FblI GTMKAC 1 cut(s) 891
Fnu4HI GCNGC 4 cut(s) 6, 603, 774, 918
FokI GGATG 3 cut(s) 31, 548, 857
Fsp4HI GCNGC 4 cut(s) 6, 603, 774, 918
FspBI CTAG 3 cut(s) 318, 926, 1056
GlaI GCGC 1 cut(s) 921
GluI GCNGC 4 cut(s) 6, 603, 774, 918
GsuI CTGGAG 1 cut(s) 483
HaeIII GGCC 2 cut(s) 183, 757
HapII CCGG 2 cut(s) 365, 642
HgaI GACGC 1 cut(s) 453
HhaI GCGC 1 cut(s) 922
Hin1I GRCGYC 1 cut(s) 445
Hin1II CATG 4 cut(s) 441, 727, 762, 1069
Hin6I GCGC 1 cut(s) 920
HinP1I GCGC 1 cut(s) 920
HinfI GANTC 8 cut(s) 329, 539, 597, 663, 690, 718, 727, 747
HpaII CCGG 2 cut(s) 365, 642
HphI GGTGA 4 cut(s) 84, 565, 748, 1071
Hpy166II GTNNAC 3 cut(s) 92, 505, 892
Hpy188I TCNGA 4 cut(s) 72, 238, 393, 615
Hpy188III TCNNGA 8 cut(s) 177, 204, 278, 630, 642, 724, 988, 1098
Hpy8I GTNNAC 3 cut(s) 92, 505, 892
HpyAV CCTTC 2 cut(s) 681, 1092
HpyCH4III ACNGT 4 cut(s) 286, 834, 949, 970
HpyCH4V TGCA 6 cut(s) 136, 470, 605, 773, 791, 824
HpyF10VI GCNNNNNNNGC 2 cut(s) 779, 788
HpyF3I CTNAG 5 cut(s) 402, 475, 523, 675, 716
Hsp92I GRCGYC 1 cut(s) 445
Hsp92II CATG 4 cut(s) 441, 727, 762, 1069
HspAI GCGC 1 cut(s) 920
Kpn2I TCCGGA 1 cut(s) 641
Kzo9I GATC 2 cut(s) 274, 638
LmnI GCTCC 1 cut(s) 956
Lsp1109I GCAGC 2 cut(s) 589, 785
LweI GCATC 1 cut(s) 785
MaeI CTAG 3 cut(s) 318, 926, 1056
MaeIII GTNAC 4 cut(s) 122, 736, 848, 928
MalI GATC 2 cut(s) 276, 640
MbiI CCGCTC 2 cut(s) 627, 917
MboI GATC 2 cut(s) 274, 638
MboII GAAGA 9 cut(s) 24, 155, 251, 272, 525, 548, 562, 577, 602
MflI RGATCY 1 cut(s) 638
MhlI GDGCHC 1 cut(s) 1004
MlsI TGGCCA 1 cut(s) 757
MluCI AATT 2 cut(s) 153, 297
MluNI TGGCCA 1 cut(s) 757
MlyI GAGTC 2 cut(s) 606, 727
Mox20I TGGCCA 1 cut(s) 757
MroI TCCGGA 1 cut(s) 641
MscI TGGCCA 1 cut(s) 757
MseI TTAA 3 cut(s) 141, 951, 972
Msp20I TGGCCA 1 cut(s) 757
MspA1I CMGCKG 1 cut(s) 907
MspI CCGG 2 cut(s) 365, 642
MspR9I CCNGG 1 cut(s) 366
Mva1269I GAATGC 1 cut(s) 824
MwoI GCNNNNNNNGC 2 cut(s) 779, 788
NciI CCSGG 1 cut(s) 366
NcoI CCATGG 2 cut(s) 437, 758
NdeII GATC 2 cut(s) 274, 638
NlaIII CATG 4 cut(s) 441, 727, 762, 1069
NlaIV GGNNCC 3 cut(s) 42, 304, 640
NmeAIII GCCGAG 1 cut(s) 5
NmuCI GTSAC 1 cut(s) 736
NspV TTCGAA 1 cut(s) 828
PagI TCATGA 1 cut(s) 723
PcsI WCGNNNNNNNCGW 1 cut(s) 807
PctI GAATGC 1 cut(s) 824
PfeI GAWTC 6 cut(s) 329, 539, 663, 690, 727, 747
PflMI CCANNNNNTGG 1 cut(s) 764
PkrI GCNGC 4 cut(s) 7, 604, 775, 919
PleI GAGTC 2 cut(s) 605, 726
PpsI GAGTC 2 cut(s) 605, 726
PspN4I GGNNCC 3 cut(s) 42, 304, 640
PspPI GGNCC 2 cut(s) 464, 767
PstI CTGCAG 2 cut(s) 472, 775
PsuI RGATCY 1 cut(s) 638
PvuII CAGCTG 1 cut(s) 907
RsaI GTAC 1 cut(s) 836
RsaNI GTAC 1 cut(s) 835
SaqAI TTAA 3 cut(s) 141, 951, 972
SatI GCNGC 4 cut(s) 6, 603, 774, 918
Sau3AI GATC 2 cut(s) 274, 638
Sau96I GGNCC 2 cut(s) 464, 767
SchI GAGTC 2 cut(s) 606, 727
ScrFI CCNGG 1 cut(s) 366
SduI GDGCHC 1 cut(s) 1004
SfaNI GCATC 1 cut(s) 785
SfcI CTRYAG 3 cut(s) 468, 699, 771
SfuI TTCGAA 1 cut(s) 828
SinI GGWCC 2 cut(s) 464, 767
SmlI CTYRAG 2 cut(s) 175, 628
SmoI CTYRAG 2 cut(s) 175, 628
SpeI ACTAGT 1 cut(s) 925
Sse9I AATT 2 cut(s) 153, 297
SsiI CCGC 3 cut(s) 594, 625, 917
SspMI CTAG 3 cut(s) 318, 926, 1056
StyD4I CCNGG 1 cut(s) 364
StyI CCWWGG 2 cut(s) 437, 758
TaaI ACNGT 4 cut(s) 286, 834, 949, 970
TaqI TCGA 1 cut(s) 828
TasI AATT 2 cut(s) 153, 297
TauI GCSGC 1 cut(s) 920
TfiI GAWTC 6 cut(s) 329, 539, 663, 690, 727, 747
Tru1I TTAA 3 cut(s) 141, 951, 972
Tru9I TTAA 3 cut(s) 141, 951, 972
TscAI CASTG 2 cut(s) 262, 1002
TseFI GTSAC 1 cut(s) 736
TseI GCWGC 3 cut(s) 5, 602, 773
Tsp45I GTSAC 1 cut(s) 736
TspDTI ATGAA 3 cut(s) 361, 478, 890
TspRI CASTG 2 cut(s) 262, 1002
Van91I CCANNNNNTGG 1 cut(s) 764
VpaK11BI GGWCC 2 cut(s) 464, 767
XmiI GTMKAC 1 cut(s) 891
XspI CTAG 3 cut(s) 318, 926, 1056
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.