FvH4_5g05620

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0009561
Physical Location & Seq
Reverse (-)
3305190 .. 3310849
5660 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g05620.t1

Sequence Viewer

Length: 1122 bp
ATGCTTGGCTCCTTCTCTTGCTCCCCATTCCCAATTGAGAAGAGGTTCTCAGATGAGCTCCTTGTCTTCAACTCATATAATTTTATTCCACCTGATGTCCAGTTGAGCTTATTACAAGCACAGTATGGCAACCAACAAAGTAGTGCTCAGCAACTACGATATGCTAAAGGCAGGTCATGCACATTCCTAAATAAAGTACTTAAGTCAAGCAAAAGGATCCCAAAGAGTCAGAGTGTACTCTTTGCTGTTTCTGAAGATCAGTCACAAAACAGTGAGACAATACCAGAAATTTTGGATCAAGAAAATGACAATCCTGATGGTGATATATCTCCTGCTAGCAATTCATATGTCCTTTTTGATGGTACTGGTGGGAAACCAGGTTTAATCTCATTTTATAACCATCCGTATAAAAGAGAAGATGATGTTTCTATAAATACTCCTGAGAGGAAGAATAGCAACCTTCTATGGCTCGTTGGTCCTGGCGTTCTTGTAGCTTCTTTCATCTTCCCCTCACTTTATCTGCGCAAATTAATTTTGACTATATTTGAGGATTCTTTGTTGACAGACTTCCTCATCTTGTTCTTCACGGAAGCTCTCTTCTACTGTGGTGTTGCGGTGTTTCTTCTTCTGATTGACAAGTTAAGGAGGCCAGCTGAACTGGAATCGGCTGCATACAGTAACAGAACCCTGGTCCCTCAGTTAGGGCAGCGAATCTTATCTGTTACTGCCTTGGCACTTAGTCTTATAATTCCTATGGTCACTATGGGCTATGTCTGGCCATGGACTGGCCCTGCAGCATCTGCTACTCTTGCTCCGTATCTGGTTGGTATAGTCGTCCAATTTGCATTTGAACAGTATGCAAGATATAAGAAGTCACCTTCATGGCCTGCTATCCCAGTCATCTTTCAAGTTTATAGATTGCATCAACTGAACAGAGCAGCACAACTGGTGACTGCTCTGTCGTTTACAGTCAAAGGAGCTGAGATGACCTCACAGAACATGGCAATAAATAGCTCGTTGGGTACACTGTTGAATGTCCTTCAATGTCTCGGAGTTATCTGCATCTGGTCTCTCTCAAGTTTTCTTATGAAGTATTTCTCTTCTAGCGCCACTACTGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

374

Amino Acids

41.46

Weight (kDa)

8.17

Isoelectric Point (pI)

47.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 396, 746
AasI GACNNNNNNGTC 1 cut(s) 958
Acc16I TGCGCA 1 cut(s) 524
Acc36I ACCTGC 1 cut(s) 162
AccB7I CCANNNNNTGG 1 cut(s) 785
AciI CCGC 1 cut(s) 614
AclWI GGATC 3 cut(s) 211, 224, 303
AcoI YGGCCR 1 cut(s) 776
AcsI RAATTY 1 cut(s) 288
AcuI CTGAAG 1 cut(s) 273
AfaI GTAC 4 cut(s) 198, 237, 364, 1024
AfiI CCNNNNNNNGG 2 cut(s) 701, 785
AflII CTTAAG 1 cut(s) 200
AgsI TTSAA 5 cut(s) 70, 851, 908, 1033, 1043
AjnI CCWGG 3 cut(s) 376, 478, 687
AluBI AGCT 7 cut(s) 58, 108, 494, 593, 653, 980, 1014
AluI AGCT 7 cut(s) 58, 108, 494, 593, 653, 980, 1014
Alw21I GWGCWC 2 cut(s) 60, 148
Alw26I GTCTC 3 cut(s) 269, 1052, 1074
AlwI GGATC 3 cut(s) 211, 224, 303
AlwNI CAGNNNCTG 1 cut(s) 800
AoxI GGCC 4 cut(s) 647, 776, 787, 884
ApeKI GCWGC 4 cut(s) 668, 706, 794, 938
ApoI RAATTY 1 cut(s) 288
AseI ATTAAT 1 cut(s) 530
Asp700I GAANNNNTTC 2 cut(s) 44, 1094
AspLEI GCGC 2 cut(s) 525, 1109
AspS9I GGNCC 3 cut(s) 476, 691, 788
AsuHPI GGTGA 3 cut(s) 332, 867, 961
AsuNHI GCTAGC 1 cut(s) 335
AvaII GGWCC 2 cut(s) 476, 691
BalI TGGCCA 1 cut(s) 778
BamHI GGATCC 1 cut(s) 216
BanII GRGCYC 1 cut(s) 60
BbsI GAAGAC 1 cut(s) 58
Bbv12I GWGCWC 2 cut(s) 60, 148
BbvI GCAGC 4 cut(s) 655, 718, 806, 950
BccI CCATC 3 cut(s) 311, 353, 408
BciT130I CCWGG 3 cut(s) 378, 480, 689
BcoDI GTCTC 3 cut(s) 269, 1052, 1074
BfaI CTAG 2 cut(s) 336, 1104
BfmI CTRYAG 1 cut(s) 792
BfoI RGCGCY 1 cut(s) 1110
BfrI CTTAAG 1 cut(s) 200
BfuAI ACCTGC 1 cut(s) 162
BisI GCNGC 4 cut(s) 669, 707, 795, 939
BlpI GCTNAGC 1 cut(s) 147
BlsI GCNGC 4 cut(s) 670, 708, 796, 940
BmcAI AGTACT 1 cut(s) 198
Bme1390I CCNGG 3 cut(s) 378, 480, 689
Bme18I GGWCC 2 cut(s) 476, 691
BmgT120I GGNCC 3 cut(s) 476, 691, 788
BmiI GGNNCC 3 cut(s) 10, 218, 693
BmrFI CCNGG 3 cut(s) 378, 480, 689
BmrI ACTGGG 1 cut(s) 890
BmsI GCATC 3 cut(s) 806, 931, 1071
BmtI GCTAGC 1 cut(s) 339
BmuI ACTGGG 1 cut(s) 890
BpiI GAAGAC 1 cut(s) 58
BplI GAGNNNNNCTC 2 cut(s) 974, 1006
Bpu1102I GCTNAGC 1 cut(s) 147
BpuEI CTTGAG 1 cut(s) 1060
BsaI GGTCTC 1 cut(s) 1074
BsaJI CCNNGG 3 cut(s) 687, 729, 779
BsaXI ACNNNNNCTCC 2 cut(s) 796, 826
Bsc4I CCNNNNNNNGG 2 cut(s) 701, 785
Bse1I ACTGG 6 cut(s) 100, 370, 663, 790, 896, 951
BseBI CCWGG 3 cut(s) 378, 480, 689
BseDI CCNNGG 3 cut(s) 687, 729, 779
BseGI GGATG 1 cut(s) 400
BseLI CCNNNNNNNGG 2 cut(s) 701, 785
BseMII CTCAG 5 cut(s) 63, 161, 432, 710, 972
BseNI ACTGG 6 cut(s) 100, 370, 663, 790, 896, 951
BseXI GCAGC 4 cut(s) 655, 718, 806, 950
BshFI GGCC 4 cut(s) 649, 778, 789, 886
BsiHKAI GWGCWC 2 cut(s) 60, 148
BslFI GGGAC 1 cut(s) 677
BslI CCNNNNNNNGG 2 cut(s) 701, 785
BsmAI GTCTC 3 cut(s) 269, 1052, 1074
BsmFI GGGAC 1 cut(s) 677
BsnI GGCC 4 cut(s) 649, 778, 789, 886
Bso31I GGTCTC 1 cut(s) 1074
Bsp1286I GDGCHC 2 cut(s) 60, 148
Bsp143I GATC 3 cut(s) 216, 256, 295
Bsp1720I GCTNAGC 1 cut(s) 147
Bsp19I CCATGG 1 cut(s) 779
BspACI CCGC 1 cut(s) 614
BspANI GGCC 4 cut(s) 649, 778, 789, 886
BspCNI CTCAG 5 cut(s) 62, 160, 433, 709, 973
BspLI GGNNCC 3 cut(s) 10, 218, 693
BspMAI CTGCAG 1 cut(s) 796
BspMI ACCTGC 1 cut(s) 162
BspOI GCTAGC 1 cut(s) 339
BspPI GGATC 3 cut(s) 211, 224, 303
BspTI CTTAAG 1 cut(s) 200
BspTNI GGTCTC 1 cut(s) 1074
BsrI ACTGG 6 cut(s) 100, 370, 663, 790, 896, 951
BssECI CCNNGG 3 cut(s) 687, 729, 779
BssMI GATC 3 cut(s) 216, 256, 295
BssT1I CCWWGG 2 cut(s) 729, 779
Bst2UI CCWGG 3 cut(s) 378, 480, 689
Bst4CI ACNGT 7 cut(s) 123, 272, 605, 677, 855, 970, 1029
Bst6I CTCTTC 3 cut(s) 35, 602, 1105
BstAFI CTTAAG 1 cut(s) 200
BstAPI GCANNNNNTGC 2 cut(s) 177, 800
BstC8I GCNNGC 3 cut(s) 337, 651, 888
BstDEI CTNAG 6 cut(s) 49, 147, 441, 696, 737, 981
BstDSI CCRYGG 1 cut(s) 779
BstENI CCTNNNNNAGG 1 cut(s) 699
BstF5I GGATG 1 cut(s) 400
BstH2I RGCGCY 1 cut(s) 1110
BstHHI GCGC 2 cut(s) 525, 1109
BstKTI GATC 3 cut(s) 219, 259, 298
BstMAI GTCTC 3 cut(s) 269, 1052, 1074
BstMBI GATC 3 cut(s) 216, 256, 295
BstMWI GCNNNNNNNGC 4 cut(s) 177, 800, 809, 1115
BstNI CCWGG 3 cut(s) 378, 480, 689
BstSCI CCNGG 3 cut(s) 376, 478, 687
BstSFI CTRYAG 1 cut(s) 792
BstV1I GCAGC 4 cut(s) 655, 718, 806, 950
BstV2I GAAGAC 1 cut(s) 58
BstX2I RGATCY 1 cut(s) 216
BstYI RGATCY 1 cut(s) 216
BsuRI GGCC 4 cut(s) 649, 778, 789, 886
BtgI CCRYGG 1 cut(s) 779
BtsCI GGATG 1 cut(s) 400
BtsIMutI CAGTG 2 cut(s) 277, 1025
BveI ACCTGC 1 cut(s) 162
Cac8I GCNNGC 3 cut(s) 337, 651, 888
CaiI CAGNNNCTG 1 cut(s) 800
CfoI GCGC 2 cut(s) 525, 1109
Cfr13I GGNCC 3 cut(s) 476, 691, 788
CsiI ACCWGGT 1 cut(s) 376
Csp6I GTAC 4 cut(s) 197, 236, 363, 1023
CviAII CATG 4 cut(s) 177, 780, 882, 1000
CviQI GTAC 4 cut(s) 197, 236, 363, 1023
DdeI CTNAG 6 cut(s) 49, 147, 441, 696, 737, 981
DpnI GATC 3 cut(s) 218, 258, 297
DpnII GATC 3 cut(s) 216, 256, 295
DrdI GACNNNNNNGTC 1 cut(s) 958
DseDI GACNNNNNNGTC 1 cut(s) 958
EaeI YGGCCR 1 cut(s) 776
Eam1104I CTCTTC 3 cut(s) 35, 602, 1105
EarI CTCTTC 3 cut(s) 35, 602, 1105
Ecl136II GAGCTC 1 cut(s) 58
Eco130I CCWWGG 2 cut(s) 729, 779
Eco24I GRGCYC 1 cut(s) 60
Eco31I GGTCTC 1 cut(s) 1074
Eco47I GGWCC 2 cut(s) 476, 691
Eco53kI GAGCTC 1 cut(s) 58
Eco57I CTGAAG 1 cut(s) 273
EcoICRI GAGCTC 1 cut(s) 58
EcoNI CCTNNNNNAGG 1 cut(s) 699
EcoRII CCWGG 3 cut(s) 376, 478, 687
EcoT14I CCWWGG 2 cut(s) 729, 779
EcoT38I GRGCYC 1 cut(s) 60
ErhI CCWWGG 2 cut(s) 729, 779
FaeI CATG 4 cut(s) 180, 783, 885, 1003
FaqI GGGAC 1 cut(s) 677
FatI CATG 4 cut(s) 176, 779, 881, 999
FauNDI CATATG 1 cut(s) 346
Fnu4HI GCNGC 4 cut(s) 669, 707, 795, 939
FokI GGATG 1 cut(s) 387
FriOI GRGCYC 1 cut(s) 60
Fsp4HI GCNGC 4 cut(s) 669, 707, 795, 939
FspBI CTAG 2 cut(s) 336, 1104
FspI TGCGCA 1 cut(s) 524
GlaI GCGC 2 cut(s) 524, 1108
GluI GCNGC 4 cut(s) 669, 707, 795, 939
HaeII RGCGCY 1 cut(s) 1110
HaeIII GGCC 4 cut(s) 649, 778, 789, 886
HhaI GCGC 2 cut(s) 525, 1109
Hin1II CATG 4 cut(s) 180, 783, 885, 1003
Hin6I GCGC 2 cut(s) 523, 1107
HinP1I GCGC 2 cut(s) 523, 1107
HincII GTYRAC 1 cut(s) 561
HindII GTYRAC 1 cut(s) 561
HinfI GANTC 4 cut(s) 226, 551, 662, 711
HphI GGTGA 3 cut(s) 332, 867, 961
Hpy166II GTNNAC 4 cut(s) 236, 561, 966, 1025
Hpy188I TCNGA 5 cut(s) 52, 231, 253, 630, 1052
Hpy188III TCNNGA 3 cut(s) 299, 314, 440
Hpy8I GTNNAC 4 cut(s) 236, 561, 966, 1025
HpyAV CCTTC 4 cut(s) 22, 470, 888, 1049
HpyCH4III ACNGT 7 cut(s) 123, 272, 605, 677, 855, 970, 1029
HpyCH4V TGCA 8 cut(s) 180, 671, 794, 845, 860, 922, 1062, 1118
HpyF10VI GCNNNNNNNGC 4 cut(s) 177, 800, 809, 1115
HpyF3I CTNAG 6 cut(s) 49, 147, 441, 696, 737, 981
Hsp92II CATG 4 cut(s) 180, 783, 885, 1003
HspAI GCGC 2 cut(s) 523, 1107
Kzo9I GATC 3 cut(s) 216, 256, 295
LmnI GCTCC 5 cut(s) 14, 26, 63, 817, 977
Lsp1109I GCAGC 4 cut(s) 655, 718, 806, 950
LweI GCATC 3 cut(s) 806, 931, 1071
MabI ACCWGGT 1 cut(s) 376
MaeI CTAG 2 cut(s) 336, 1104
MaeIII GTNAC 6 cut(s) 261, 677, 721, 757, 873, 949
MalI GATC 3 cut(s) 218, 258, 297
MboI GATC 3 cut(s) 216, 256, 295
MfeI CAATTG 1 cut(s) 33
MflI RGATCY 1 cut(s) 216
MhlI GDGCHC 2 cut(s) 60, 148
MlsI TGGCCA 1 cut(s) 778
MluCI AATT 8 cut(s) 33, 79, 288, 340, 527, 531, 747, 839
MluNI TGGCCA 1 cut(s) 778
MlyI GAGTC 1 cut(s) 235
MnlI CCTC 8 cut(s) 36, 438, 520, 541, 581, 639, 705, 1000
Mox20I TGGCCA 1 cut(s) 778
MroXI GAANNNNTTC 2 cut(s) 44, 1094
MscI TGGCCA 1 cut(s) 778
MseI TTAA 4 cut(s) 201, 383, 530, 641
MslI CAYNNNNRTG 1 cut(s) 880
Msp20I TGGCCA 1 cut(s) 778
MspA1I CMGCKG 1 cut(s) 653
MspCI CTTAAG 1 cut(s) 200
MspR9I CCNGG 3 cut(s) 378, 480, 689
MunI CAATTG 1 cut(s) 33
MvaI CCWGG 3 cut(s) 378, 480, 689
MwoI GCNNNNNNNGC 4 cut(s) 177, 800, 809, 1115
NcoI CCATGG 1 cut(s) 779
NdeI CATATG 1 cut(s) 346
NdeII GATC 3 cut(s) 216, 256, 295
NheI GCTAGC 1 cut(s) 335
NlaIII CATG 4 cut(s) 180, 783, 885, 1003
NlaIV GGNNCC 3 cut(s) 10, 218, 693
NmuCI GTSAC 4 cut(s) 261, 757, 873, 949
NsbI TGCGCA 1 cut(s) 524
PdmI GAANNNNTTC 2 cut(s) 44, 1094
PfeI GAWTC 3 cut(s) 551, 662, 711
PflMI CCANNNNNTGG 1 cut(s) 785
PkrI GCNGC 4 cut(s) 670, 708, 796, 940
PleI GAGTC 1 cut(s) 234
PpsI GAGTC 1 cut(s) 234
PshBI ATTAAT 1 cut(s) 530
PsiI TTATAA 2 cut(s) 396, 746
Psp124BI GAGCTC 1 cut(s) 60
Psp6I CCWGG 3 cut(s) 376, 478, 687
PspGI CCWGG 3 cut(s) 376, 478, 687
PspN4I GGNNCC 3 cut(s) 10, 218, 693
PspPI GGNCC 3 cut(s) 476, 691, 788
PstI CTGCAG 1 cut(s) 796
PstNI CAGNNNCTG 1 cut(s) 800
PsuI RGATCY 1 cut(s) 216
PvuII CAGCTG 1 cut(s) 653
RsaI GTAC 4 cut(s) 198, 237, 364, 1024
RsaNI GTAC 4 cut(s) 197, 236, 363, 1023
RseI CAYNNNNRTG 1 cut(s) 880
SacI GAGCTC 1 cut(s) 60
SaqAI TTAA 4 cut(s) 201, 383, 530, 641
SatI GCNGC 4 cut(s) 669, 707, 795, 939
Sau3AI GATC 3 cut(s) 216, 256, 295
Sau96I GGNCC 3 cut(s) 476, 691, 788
ScaI AGTACT 1 cut(s) 198
SchI GAGTC 1 cut(s) 235
ScrFI CCNGG 3 cut(s) 378, 480, 689
SduI GDGCHC 2 cut(s) 60, 148
SexAI ACCWGGT 1 cut(s) 376
SfaNI GCATC 3 cut(s) 806, 931, 1071
SfcI CTRYAG 1 cut(s) 792
SinI GGWCC 2 cut(s) 476, 691
SmiMI CAYNNNNRTG 1 cut(s) 880
SmlI CTYRAG 2 cut(s) 200, 1075
SmoI CTYRAG 2 cut(s) 200, 1075
Sse9I AATT 8 cut(s) 33, 79, 288, 340, 527, 531, 747, 839
SsiI CCGC 1 cut(s) 614
SspMI CTAG 2 cut(s) 336, 1104
SstI GAGCTC 1 cut(s) 60
StyD4I CCNGG 3 cut(s) 376, 478, 687
StyI CCWWGG 2 cut(s) 729, 779
TaaI ACNGT 7 cut(s) 123, 272, 605, 677, 855, 970, 1029
TasI AATT 8 cut(s) 33, 79, 288, 340, 527, 531, 747, 839
TatI WGTACW 2 cut(s) 196, 235
TfiI GAWTC 3 cut(s) 551, 662, 711
Tru1I TTAA 4 cut(s) 201, 383, 530, 641
Tru9I TTAA 4 cut(s) 201, 383, 530, 641
TscAI CASTG 2 cut(s) 277, 1032
TseFI GTSAC 4 cut(s) 261, 757, 873, 949
TseI GCWGC 4 cut(s) 668, 706, 794, 938
Tsp45I GTSAC 4 cut(s) 261, 757, 873, 949
TspDTI ATGAA 4 cut(s) 333, 490, 870, 1103
TspGWI ACGGA 3 cut(s) 393, 602, 804
TspRI CASTG 2 cut(s) 277, 1032
Van91I CCANNNNNTGG 1 cut(s) 785
Vha464I CTTAAG 1 cut(s) 200
VpaK11BI GGWCC 2 cut(s) 476, 691
VspI ATTAAT 1 cut(s) 530
XagI CCTNNNNNAGG 1 cut(s) 699
XapI RAATTY 1 cut(s) 288
XmnI GAANNNNTTC 2 cut(s) 44, 1094
XspI CTAG 2 cut(s) 336, 1104
ZrmI AGTACT 1 cut(s) 198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.