AT5G63720

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009441
Physical Location & Seq
Forward (+)
25506279 .. 25508017
1739 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G63720.1

Sequence Viewer

Length: 1479 bp
ATGGAGCCCAATAACGTAGAGCGTAATCTTCAATCTCTGAGAAGATTATACAGTCTTCTCGTAGCAAATGCAAGGAACGAATATATTCCTGAGGCATATAAGTTGGACGACAACACTCAGTTTTTATTAAAGCGATTGCTCGATTTTGCTTCCCACGAACACTTTGTTACACAATCAAACTTACTTGCAACACAATTACGTGTGTTTCCTAAAACGGTTTTACACGGTGCAAAGCCGTCGAACGTGGCGGATTCTTCTGAGACGACGCCACCTGCAATGTTGTCTCAGATCAATGGTTCTCATATTACGCGAGTTAGTAAACCATTGGAGGCGAAAGGAACATTGCAGAGAGATCTGAGAGTTGATCAGATTCGGAGCAATCCTAGTAAGGATGTTTTGACAGAAGAAGTCGTTGATGCAATAGAGCAAATCGATACACAACTCTCCGCTTTAAGTTTCGTTTCTAGTCGTGTTGACTCTGATGAAAGAACAAGGTCTGTCAAGTCCTTTGTAACTCCTCCCACTGAAGAGTGTAGACAAAACAGTCAAGCTTCTATGCCTTGTTTAAGAAGCAATTACATGACGGTGAGTCAGAAAAGTGTGATTAGTGGTGCAGAAGTAACTTTCCCTTATAATGATCAGTTAGTTCGGGTTACGTCACCACCGCAGCCATTACCGCCTCGTGCTGTGAGTGGTTTCAAGAAGCCAAATCAAAGTAATAGAGCGTCACAAAAGATGCCGATTATGAAACCTACTTTGATGGATCAAGAAACCGAAACGTTTGATGATGATTCTTCTGAAACAGAAGCTGACCAAACACCGTCTGCGACAGGTTCTGAGTCGGAAGACGAAGAAGTTTCAACTAGTCAAGAATACTCTGGGGAAACAGGGTCAAGCTCTGGCTCGGAGTGGGAAACTCAAGCAGAGAATGACACCGAGTCTAAGTCAGAATCATCATATCCACCACAGAATGATGACTCTGTCTCTGAAGTCAGCACTTCTCCTCCGCATACAGACAGGGACACATCAAGAGAACCTGGAAAACAGAGAAGAAATGTAATGGGGCGGTTTAAAAGGATAAAGAATAAGATAGGACAAATATTTCACCATCATCACCACCACCACCACCATCATCATCACCACGACAAGGAAAAGCCATCAGCTTGGAATAAGTTGCAGAGTAAATTTCACCATAAGCACCAAGAGAAATCAAAAGAGAGGAAAAGACCGATGTCAGAATCCAAAGGTCTTACTACACATAAGCAGCAACATCAAGGTGGCCACTTTCATGCGCTAGTGGAAGGGTTGGTGAGACACAGAAAACACTCAAAAAAACAAAAGCATCAGCTGAAGAGTGATGCCAAGAAAACAGAGTGGTGGAAATTATTGAAGAAGCGTCAAGGTGGTGGCGTGAAGATTCCCAAAAGAGGACGTGTGAAGTTGGGAAAAAAAAAACATTATTTGTCCAAAAATGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0007275 GO:0008150 GO:0009555 GO:0009566 GO:0009567 GO:0019953 GO:0022414 GO:0032501 GO:0032502 GO:0044703 GO:0048229 GO:0048856 GO:0051704
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

492

Amino Acids

55.95

Weight (kDa)

9.44

Isoelectric Point (pI)

56.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 633
AarI CACCTGC 1 cut(s) 280
AasI GACNNNNNNGTC 1 cut(s) 543
Acc36I ACCTGC 1 cut(s) 280
AccI GTMKAC 1 cut(s) 535
AccII CGCG 1 cut(s) 310
AciI CCGC 6 cut(s) 248, 447, 665, 677, 1007, 1066
AclI AACGTT 1 cut(s) 779
AclWI GGATC 1 cut(s) 771
AcoI YGGCCR 1 cut(s) 1279
AcsI RAATTY 1 cut(s) 1184
AcuI CTGAAG 3 cut(s) 546, 1008, 1370
AcyI GRCGYC 1 cut(s) 266
AfiI CCNNNNNNNGG 2 cut(s) 1147, 1427
AflIII ACRYGT 2 cut(s) 199, 1432
AgsI TTSAA 4 cut(s) 32, 700, 861, 1390
AhdI GACNNNNNGTC 2 cut(s) 588, 937
AhlI ACTAGT 1 cut(s) 863
AjiI CACGTC 1 cut(s) 1433
AjnI CCWGG 1 cut(s) 1036
AluBI AGCT 5 cut(s) 551, 809, 897, 1163, 1348
AluI AGCT 5 cut(s) 551, 809, 897, 1163, 1348
Alw26I GTCTC 4 cut(s) 254, 288, 988, 1306
AlwI GGATC 1 cut(s) 771
AlwNI CAGNNNCTG 2 cut(s) 809, 836
AoxI GGCC 1 cut(s) 1279
ApeKI GCWGC 2 cut(s) 667, 1264
ApoI RAATTY 1 cut(s) 1184
Asp700I GAANNNNTTC 1 cut(s) 84
AspLEI GCGC 1 cut(s) 1294
AsuHPI GGTGA 7 cut(s) 598, 651, 1097, 1106, 1130, 1181, 1321
AxyI CCTNAGG 1 cut(s) 90
BalI TGGCCA 1 cut(s) 1281
BanII GRGCYC 1 cut(s) 9
BauI CACGAG 1 cut(s) 681
BbsI GAAGAC 2 cut(s) 47, 852
BbvI GCAGC 2 cut(s) 679, 1276
BccI CCATC 4 cut(s) 754, 1116, 1137, 1165
BceAI ACGGC 1 cut(s) 220
BcgI CGANNNNNNTGC 2 cut(s) 219, 253
BciT130I CCWGG 1 cut(s) 1038
BclI TGATCA 2 cut(s) 364, 637
BcoDI GTCTC 4 cut(s) 254, 288, 988, 1306
BcuI ACTAGT 1 cut(s) 863
BfaI CTAG 4 cut(s) 384, 465, 864, 1295
BfuAI ACCTGC 1 cut(s) 280
BglII AGATCT 1 cut(s) 352
BisI GCNGC 2 cut(s) 668, 1265
BlsI GCNGC 2 cut(s) 669, 1266
Bme1390I CCNGG 1 cut(s) 1038
BmeRI GACNNNNNGTC 2 cut(s) 588, 937
BmgBI CACGTC 1 cut(s) 1433
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 1 cut(s) 1038
BmsI GCATC 4 cut(s) 406, 726, 1348, 1351
BoxI GACNNNNGTC 1 cut(s) 1231
BpiI GAAGAC 2 cut(s) 47, 852
BpuEI CTTGAG 1 cut(s) 903
Bsa29I ATCGAT 1 cut(s) 432
BsaAI YACGTR 1 cut(s) 200
BsaHI GRCGYC 1 cut(s) 266
BsaXI ACNNNNNCTCC 2 cut(s) 988, 1018
Bsc4I CCNNNNNNNGG 2 cut(s) 1147, 1427
Bse21I CCTNAGG 1 cut(s) 90
Bse3DI GCAATG 2 cut(s) 282, 341
BseBI CCWGG 1 cut(s) 1038
BseCI ATCGAT 1 cut(s) 432
BseGI GGATG 1 cut(s) 397
BseLI CCNNNNNNNGG 2 cut(s) 1147, 1427
BseMI GCAATG 2 cut(s) 282, 341
BseMII CTCAG 7 cut(s) 29, 81, 131, 249, 299, 347, 828
BseRI GAGGAG 2 cut(s) 507, 993
BseXI GCAGC 2 cut(s) 679, 1276
BsgI GTGCAG 1 cut(s) 633
Bsh1236I CGCG 1 cut(s) 310
BshFI GGCC 1 cut(s) 1281
BshVI ATCGAT 1 cut(s) 432
BslFI GGGAC 1 cut(s) 1034
BslI CCNNNNNNNGG 2 cut(s) 1147, 1427
BsmAI GTCTC 4 cut(s) 254, 288, 988, 1306
BsmBI CGTCTC 1 cut(s) 254
BsmFI GGGAC 1 cut(s) 1034
BsnI GGCC 1 cut(s) 1281
Bsp1286I GDGCHC 1 cut(s) 9
Bsp143I GATC 5 cut(s) 288, 352, 364, 637, 763
BspACI CCGC 6 cut(s) 248, 447, 665, 677, 1007, 1066
BspANI GGCC 1 cut(s) 1281
BspCNI CTCAG 7 cut(s) 30, 82, 130, 250, 298, 348, 829
BspDI ATCGAT 1 cut(s) 432
BspFNI CGCG 1 cut(s) 310
BspLI GGNNCC 1 cut(s) 6
BspMI ACCTGC 1 cut(s) 280
BspPI GGATC 1 cut(s) 771
BsrDI GCAATG 2 cut(s) 282, 341
BssMI GATC 5 cut(s) 288, 352, 364, 637, 763
BssNI GRCGYC 1 cut(s) 266
BssSI CACGAG 1 cut(s) 681
Bst2BI CACGAG 1 cut(s) 681
Bst2UI CCWGG 1 cut(s) 1038
Bst4CI ACNGT 6 cut(s) 53, 217, 227, 545, 586, 822
Bst6I CTCTTC 2 cut(s) 522, 1346
BstACI GRCGYC 1 cut(s) 266
BstBAI YACGTR 1 cut(s) 200
BstDEI CTNAG 8 cut(s) 38, 90, 117, 258, 285, 356, 837, 942
BstF5I GGATG 1 cut(s) 397
BstFNI CGCG 1 cut(s) 310
BstHHI GCGC 1 cut(s) 1294
BstKTI GATC 5 cut(s) 291, 355, 367, 640, 766
BstMAI GTCTC 4 cut(s) 254, 288, 988, 1306
BstMBI GATC 5 cut(s) 288, 352, 364, 637, 763
BstMWI GCNNNNNNNGC 1 cut(s) 676
BstNI CCWGG 1 cut(s) 1038
BstPAI GACNNNNGTC 1 cut(s) 1231
BstSCI CCNGG 1 cut(s) 1036
BstUI CGCG 1 cut(s) 310
BstV1I GCAGC 2 cut(s) 679, 1276
BstV2I GAAGAC 2 cut(s) 47, 852
BstX2I RGATCY 1 cut(s) 352
BstXI CCANNNNNNTGG 1 cut(s) 1164
BstYI RGATCY 1 cut(s) 352
Bsu15I ATCGAT 1 cut(s) 432
Bsu36I CCTNAGG 1 cut(s) 90
BsuRI GGCC 1 cut(s) 1281
BsuTUI ATCGAT 1 cut(s) 432
BtrI CACGTC 1 cut(s) 1433
BtsCI GGATG 1 cut(s) 397
BtsIMutI CAGTG 1 cut(s) 522
BveI ACCTGC 1 cut(s) 280
CaiI CAGNNNCTG 2 cut(s) 809, 836
CfoI GCGC 1 cut(s) 1294
ClaI ATCGAT 1 cut(s) 432
CseI GACGC 3 cut(s) 274, 714, 1385
CviAII CATG 2 cut(s) 580, 1289
DdeI CTNAG 8 cut(s) 38, 90, 117, 258, 285, 356, 837, 942
DpnI GATC 5 cut(s) 290, 354, 366, 639, 765
DpnII GATC 5 cut(s) 288, 352, 364, 637, 763
DraI TTTAAA 1 cut(s) 1072
DrdI GACNNNNNNGTC 1 cut(s) 543
DriI GACNNNNNGTC 2 cut(s) 588, 937
DseDI GACNNNNNNGTC 1 cut(s) 543
EaeI YGGCCR 1 cut(s) 1279
Eam1104I CTCTTC 2 cut(s) 522, 1346
Eam1105I GACNNNNNGTC 2 cut(s) 588, 937
EarI CTCTTC 2 cut(s) 522, 1346
EciI GGCGGA 1 cut(s) 263
Eco24I GRGCYC 1 cut(s) 9
Eco57I CTGAAG 3 cut(s) 546, 1008, 1370
Eco81I CCTNAGG 1 cut(s) 90
EcoRII CCWGG 1 cut(s) 1036
EcoT38I GRGCYC 1 cut(s) 9
Esp3I CGTCTC 1 cut(s) 254
FaeI CATG 2 cut(s) 583, 1292
FaqI GGGAC 1 cut(s) 1034
FatI CATG 2 cut(s) 579, 1288
FbaI TGATCA 2 cut(s) 364, 637
FblI GTMKAC 1 cut(s) 535
Fnu4HI GCNGC 2 cut(s) 668, 1265
FokI GGATG 1 cut(s) 404
FriOI GRGCYC 1 cut(s) 9
Fsp4HI GCNGC 2 cut(s) 668, 1265
FspBI CTAG 4 cut(s) 384, 465, 864, 1295
GlaI GCGC 1 cut(s) 1293
GluI GCNGC 2 cut(s) 668, 1265
HaeIII GGCC 1 cut(s) 1281
HgaI GACGC 3 cut(s) 274, 714, 1385
HhaI GCGC 1 cut(s) 1294
Hin1I GRCGYC 1 cut(s) 266
Hin1II CATG 2 cut(s) 583, 1292
Hin6I GCGC 1 cut(s) 1292
HinP1I GCGC 1 cut(s) 1292
HincII GTYRAC 1 cut(s) 475
HindII GTYRAC 1 cut(s) 475
HindIII AAGCTT 1 cut(s) 549
HphI GGTGA 7 cut(s) 598, 651, 1097, 1106, 1130, 1181, 1321
Hpy166II GTNNAC 3 cut(s) 320, 475, 536
Hpy188III TCNNGA 5 cut(s) 89, 700, 767, 869, 1029
Hpy8I GTNNAC 3 cut(s) 320, 475, 536
Hpy99I CGWCG 2 cut(s) 241, 268
HpyAV CCTTC 1 cut(s) 1295
HpyCH4III ACNGT 6 cut(s) 53, 217, 227, 545, 586, 822
HpyCH4IV ACGT 6 cut(s) 15, 199, 243, 656, 779, 1432
HpyCH4V TGCA 8 cut(s) 71, 188, 230, 275, 346, 419, 614, 1177
HpyF10VI GCNNNNNNNGC 1 cut(s) 676
HpyF3I CTNAG 8 cut(s) 38, 90, 117, 258, 285, 356, 837, 942
HpySE526I ACGT 6 cut(s) 15, 199, 243, 656, 779, 1432
Hsp92I GRCGYC 1 cut(s) 266
Hsp92II CATG 2 cut(s) 583, 1292
HspAI GCGC 1 cut(s) 1292
Ksp22I TGATCA 2 cut(s) 364, 637
Kzo9I GATC 5 cut(s) 288, 352, 364, 637, 763
LmnI GCTCC 2 cut(s) 4, 375
LpnPI CCDG 9 cut(s) 102, 285, 816, 864, 873, 885, 1003, 1023, 1050
Lsp1109I GCAGC 2 cut(s) 679, 1276
LweI GCATC 4 cut(s) 406, 726, 1348, 1351
MaeI CTAG 4 cut(s) 384, 465, 864, 1295
MaeII ACGT 6 cut(s) 15, 199, 243, 656, 779, 1432
MaeIII GTNAC 6 cut(s) 166, 511, 619, 652, 657, 726
MalI GATC 5 cut(s) 290, 354, 366, 639, 765
MboI GATC 5 cut(s) 288, 352, 364, 637, 763
MflI RGATCY 1 cut(s) 352
MhlI GDGCHC 1 cut(s) 9
MlsI TGGCCA 1 cut(s) 1281
MluCI AATT 4 cut(s) 194, 574, 1184, 1382
MluNI TGGCCA 1 cut(s) 1281
MlyI GAGTC 5 cut(s) 470, 598, 848, 947, 971
MmeI TCCRAC 2 cut(s) 84, 822
MnlI CCTC 7 cut(s) 85, 322, 528, 690, 1014, 1212, 1421
Mox20I TGGCCA 1 cut(s) 1281
MroXI GAANNNNTTC 1 cut(s) 84
MscI TGGCCA 1 cut(s) 1281
MseI TTAA 5 cut(s) 128, 452, 566, 1071, 1477
MslI CAYNNNNRTG 3 cut(s) 584, 1275, 1287
Msp20I TGGCCA 1 cut(s) 1281
MspA1I CMGCKG 1 cut(s) 1348
MspR9I CCNGG 1 cut(s) 1038
MvaI CCWGG 1 cut(s) 1038
MvnI CGCG 1 cut(s) 310
MwoI GCNNNNNNNGC 1 cut(s) 676
NdeII GATC 5 cut(s) 288, 352, 364, 637, 763
NlaIII CATG 2 cut(s) 583, 1292
NlaIV GGNNCC 1 cut(s) 6
NmuCI GTSAC 2 cut(s) 657, 726
PaqCI CACCTGC 1 cut(s) 280
PdmI GAANNNNTTC 1 cut(s) 84
PfeI GAWTC 6 cut(s) 251, 370, 791, 950, 1238, 1417
PflFI GACNNNGTC 1 cut(s) 980
PkrI GCNGC 2 cut(s) 669, 1266
PleI GAGTC 5 cut(s) 470, 597, 847, 946, 971
PpsI GAGTC 5 cut(s) 470, 597, 847, 946, 971
Ppu21I YACGTR 1 cut(s) 200
PshAI GACNNNNGTC 1 cut(s) 1231
PsiI TTATAA 1 cut(s) 633
Psp1406I AACGTT 1 cut(s) 779
Psp6I CCWGG 1 cut(s) 1036
PspGI CCWGG 1 cut(s) 1036
PspN4I GGNNCC 1 cut(s) 6
PstNI CAGNNNCTG 2 cut(s) 809, 836
PsuI RGATCY 1 cut(s) 352
PsyI GACNNNGTC 1 cut(s) 980
PvuII CAGCTG 1 cut(s) 1348
RseI CAYNNNNRTG 3 cut(s) 584, 1275, 1287
SaqAI TTAA 5 cut(s) 128, 452, 566, 1071, 1477
SatI GCNGC 2 cut(s) 668, 1265
Sau3AI GATC 5 cut(s) 288, 352, 364, 637, 763
SchI GAGTC 5 cut(s) 470, 598, 848, 947, 971
ScrFI CCNGG 1 cut(s) 1038
SduI GDGCHC 1 cut(s) 9
SfaNI GCATC 4 cut(s) 406, 726, 1348, 1351
SmiMI CAYNNNNRTG 3 cut(s) 584, 1275, 1287
SmlI CTYRAG 1 cut(s) 918
SmoI CTYRAG 1 cut(s) 918
SpeI ACTAGT 1 cut(s) 863
Sse9I AATT 4 cut(s) 194, 574, 1184, 1382
SsiI CCGC 6 cut(s) 248, 447, 665, 677, 1007, 1066
SspI AATATT 1 cut(s) 1101
SspMI CTAG 4 cut(s) 384, 465, 864, 1295
StyD4I CCNGG 1 cut(s) 1036
TaaI ACNGT 6 cut(s) 53, 217, 227, 545, 586, 822
TaiI ACGT 6 cut(s) 18, 202, 246, 659, 782, 1435
TaqI TCGA 3 cut(s) 141, 239, 432
TaqII GACCGA 1 cut(s) 1243
TasI AATT 4 cut(s) 194, 574, 1184, 1382
TfiI GAWTC 6 cut(s) 251, 370, 791, 950, 1238, 1417
Tru1I TTAA 5 cut(s) 128, 452, 566, 1071, 1477
Tru9I TTAA 5 cut(s) 128, 452, 566, 1071, 1477
TscAI CASTG 1 cut(s) 529
TseFI GTSAC 2 cut(s) 657, 726
TseI GCWGC 2 cut(s) 667, 1264
Tsp45I GTSAC 2 cut(s) 657, 726
TspDTI ATGAA 3 cut(s) 498, 761, 1277
TspRI CASTG 1 cut(s) 529
Tth111I GACNNNGTC 1 cut(s) 980
XapI RAATTY 1 cut(s) 1184
XmiI GTMKAC 1 cut(s) 535
XmnI GAANNNNTTC 1 cut(s) 84
XspI CTAG 4 cut(s) 384, 465, 864, 1295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.