pycom15g12540

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0009441
Physical Location & Seq
Reverse (-)
8553267 .. 8555945
2679 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g12540.3

Sequence Viewer

Length: 2427 bp
ATGAATCCTGTGGAACTCTTGTCTGATCTCTATGCGTTGAGACAGTTATATGGACTCCTCCAAAATGGCGAAGGCTGGCCGCAACCTATAAATTCAAACAATGTGGAAAAAGCTTTGGACACGAGAGCGCGACAATTGTTAAAAGATCTTCTAGATGATGCTGCTGAAGGGATTTTCAATACTCATTTGAAGATGATAACAACTCAGGCAGCTGTTAGTAGCAAACCTTGTCCCTTTCAACCAGAGCAACCCAAGGCTGTGCCTAAGAAGAATTCTCCTCCTAAGAAGAATTCTCCTCTTTCCCCAACAGTTACAGTGAAATCTTCTCCTAACACAGTCTTGAATGATGATGGAAAGGATTCTAAAAAGTACATGTTCGCTCCTGAGGAGGATGGGTCACTGAAGAAGCTGAAGCCTGAGAGAGAAATTGCGGAGTCTAAAGTAATCAACTCATCGGACCTGCCTGAACAGAAAAAGAAAGTGTGTCGAATCTGCTTAGAAAACAATCTGAAGAAACAGTACTCAGCCAAGGATGCAAAATTCTCCAATGACAGAGTCGAGGTACCTGACAAAAACAGTGTTAGTCAAGATAAACAACATGATTGGCGTAGTCATCTCAATGTTCTCGATGAGCATGCTAAAAGATTACAAGAATCTAATTCAAAGTCCACAGAGAGAGGGAATGACATCAGTGGAACTCTTGGAAATCCAAGCATTGTTAGTTTTGGTGTTTGTTCCCCAATATCTCCAACAAGAATGGCTAGCAAGCAAGAAGTCGGTTTGTACCCGATTGGCCAGAGCGAGGAAAAGTCAGATGAGGGCAGTGATTACTCTAAAGTAGTTGCAAATGCAATCGAACAAATTGAGTTGCGCATTTTGGCTGTGCAGTTGAGTTCTTTTGGGATGGCGAATTCTTTCAACAATGATGCTAATGAACATTATTCTTTCAAGCGTGACATTTCATTTAGTCCTTCTACTCCTACTCCTACAGCGCAGATAGAAGAACAGGTAGCTCCTACTACTATTAGTACTACTATTACTAGAAGAGAGCTGAGTGGTATGGACAAGTCTTTTCCTTTGTTGGGTGGAACTCAGTTGCTAACTCGTCAGGCGAGTCAATTACTGTCTCAAGATCCGACTGGGAGGTATGCTTCTAATTCCAGGGAACATGGTTCCTCTAAGGGCCTGATGGAGAGACTTGGATCGCGGAGGTTCCCTCCGAGTCAACCAGTGAGTCGGGATGAGAAGATGGCAGGTCATGTGAATGGTCTAAGGGTCCCTCCTCCAAATCAGGCTCCTATGCACGTTAAGACACCTGATCAAACTTGGAAAACCACTCCAGTCCAAAATGCAACGACTAGAAAAATAGGGGCTGCAAGATCTAATCGGTTCGTGCCACCTGAGTCCATAGCTTCTTCAGTTGGACATGGACCTAGTTGTAAGAAGTTGATGAGTCAAACTCGGAAAATTAGTCTAGTGACAAACAAGAGACATCATACAATGACCGTGAAGCCAATTTTGTTAGATGAACACAAGCCTAGTGGGACACACAAAATGTCTTCTGCAGGCCGACAGAAGAAGAGAATGCCACGAAAGCAAGTGTTGGATATGGAGGAGGAGACTAGCTCAGGTTGTACTGATTCCGAAACTTGGTCGACGTGGGCAAGTGAGACAAATTCTGAAGCTGAAAGCAGCGCTGGTAGTAATGAATACTCAAAAGAGGATTCCTGGAGTCGAGGCCTGTCTAGGAGACGTACTCGTGATATTCATATGAGAATGAGAGGTGATCTCTCTTCCTCAACAAGGTCGATAAATACGGCGGCAGAAGTAAGCGCTAGTAGTAGTTCACGGAATGGTAGCTGTAGCTCATCAATGGAGGAGGAGGATGATCTCTATAGCCGTAAAAGATATCCGGAGAAGTCCGTTGGACGATTTAGGAGGCTCAAGAACAGGTTGGGACTCATATTTCACCACCATCACCATCACCATCACCACTATGACGGAAATGGTAATAGTGATGACGATCGCGGCCGTTCTGGGTGGAAACACCTACGGAGGAAGATGTTTGACAGCCACAAAAACCCGCATGGCATCCGAGAAAGCAGAGGGAGCAAAAAATCATTGGTGAAAGTGAAAAACATGTTGCATAATAAGCAGGGGGAGGTTGGGCATTTTCATGCGCTGTTGGAAGGGCTGTTGAGGCACATAAGGCATTCGAAGAAATCCAGTAACTACTTGTCAAAGGGAGGAGGAGTAGGAGGAGGAGGAGGAGGAGGAAGAAGTTGCATTATTAGGAAGAAGAAGAAGTTGCATTGGTGGCAAATGTTTCGACGCAGGCGAGTCAAGATGCAGCCTAATAAGGCTAAAAGAGGGCGTGTCAAACTCGGATTCACTGCTAAAAGACGGCCAAAGCTTAAAATCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0007275 GO:0008150 GO:0009555 GO:0009566 GO:0009567 GO:0019953 GO:0022414 GO:0032501 GO:0032502 GO:0044703 GO:0048229 GO:0048856 GO:0051704
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

809

Amino Acids

90.47

Weight (kDa)

9.98

Isoelectric Point (pI)

61.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 872
Acc36I ACCTGC 2 cut(s) 468, 1244
Acc65I GGTACC 1 cut(s) 562
AccB1I GGYRCC 1 cut(s) 562
AccI GTMKAC 1 cut(s) 1655
AccII CGCG 3 cut(s) 130, 1207, 2028
AccIII TCCGGA 1 cut(s) 1912
AciI CCGC 6 cut(s) 80, 431, 1207, 1820, 2028, 2084
AclWI GGATC 2 cut(s) 1127, 1210
AcoI YGGCCR 4 cut(s) 77, 793, 2029, 2405
AcsI RAATTY 6 cut(s) 91, 271, 289, 539, 910, 1675
AcuI CTGAAG 6 cut(s) 186, 422, 431, 530, 1401, 1701
AfaI GTAC 7 cut(s) 371, 521, 564, 785, 1030, 1636, 1756
AfeI AGCGCT 2 cut(s) 1696, 1834
AfiI CCNNNNNNNGG 4 cut(s) 802, 1082, 1650, 1803
AflIII ACRYGT 2 cut(s) 372, 2139
AgsI TTSAA 8 cut(s) 96, 178, 190, 239, 343, 663, 919, 949
AjiI CACGTC 1 cut(s) 1659
AjnI CCWGG 2 cut(s) 1160, 1727
Alw26I GTCTC 7 cut(s) 34, 1131, 1189, 1483, 1613, 1664, 1744
AlwI GGATC 2 cut(s) 1127, 1210
Aor13HI TCCGGA 1 cut(s) 1912
Aor51HI AGCGCT 2 cut(s) 1696, 1834
AoxI GGCC 7 cut(s) 77, 793, 1183, 1567, 1738, 2029, 2405
ApeKI GCWGC 5 cut(s) 161, 209, 1373, 1692, 2350
ApoI RAATTY 6 cut(s) 91, 271, 289, 539, 910, 1675
Asp700I GAANNNNTTC 2 cut(s) 358, 914
Asp718I GGTACC 1 cut(s) 562
AspLEI GCGC 6 cut(s) 130, 873, 994, 1697, 1835, 2182
AspS9I GGNCC 4 cut(s) 457, 1183, 1276, 1430
AsuHPI GGTGA 6 cut(s) 1796, 1961, 1970, 1976, 1982, 2137
AsuII TTCGAA 1 cut(s) 2216
AsuNHI GCTAGC 1 cut(s) 761
AvaII GGWCC 3 cut(s) 457, 1276, 1430
AxyI CCTNAGG 1 cut(s) 384
BalI TGGCCA 1 cut(s) 795
BanI GGYRCC 1 cut(s) 562
BarI GAAGNNNNNNTAC 2 cut(s) 503, 535
BauI CACGAG 2 cut(s) 121, 1758
BbsI GAAGAC 1 cut(s) 1551
BbvI GCAGC 5 cut(s) 148, 221, 1360, 1704, 2362
BccI CCATC 8 cut(s) 344, 386, 898, 1183, 1243, 1983, 1989, 1995
BceAI ACGGC 4 cut(s) 1833, 1884, 2016, 2420
BcgI CGANNNNNNTGC 4 cut(s) 617, 651, 2309, 2343
BciT130I CCWGG 2 cut(s) 1162, 1729
BclI TGATCA 1 cut(s) 1318
BcoDI GTCTC 7 cut(s) 34, 1131, 1189, 1483, 1613, 1664, 1744
BfmI CTRYAG 4 cut(s) 987, 1563, 1861, 1894
BfoI RGCGCY 2 cut(s) 1698, 1836
BfuAI ACCTGC 2 cut(s) 468, 1244
BglII AGATCT 2 cut(s) 145, 1379
BisI GCNGC 8 cut(s) 80, 162, 210, 1374, 1693, 1821, 2029, 2351
BlsI GCNGC 8 cut(s) 81, 163, 211, 1375, 1694, 1822, 2030, 2352
BmcAI AGTACT 2 cut(s) 521, 1030
Bme1390I CCNGG 2 cut(s) 1162, 1729
Bme18I GGWCC 3 cut(s) 457, 1276, 1430
BmgBI CACGTC 1 cut(s) 1659
BmgT120I GGNCC 4 cut(s) 457, 1183, 1276, 1430
BmiI GGNNCC 6 cut(s) 564, 1174, 1214, 1277, 1278, 1296
BmrFI CCNGG 2 cut(s) 1162, 1729
BmrI ACTGGG 1 cut(s) 1149
BmsI GCATC 5 cut(s) 148, 523, 916, 2100, 2337
BmtI GCTAGC 1 cut(s) 765
BmuI ACTGGG 1 cut(s) 1149
BpiI GAAGAC 1 cut(s) 1551
BpmI CTGGAG 2 cut(s) 1323, 1750
Bpu10I CCTNAGC 1 cut(s) 1627
Bpu14I TTCGAA 1 cut(s) 2216
BpuEI CTTGAG 2 cut(s) 1113, 1928
BsaBI GATNNNNATC 1 cut(s) 2022
BsaJI CCNNGG 3 cut(s) 252, 528, 1161
BsaWI WCCGGW 1 cut(s) 1912
BsaXI ACNNNNNCTCC 6 cut(s) 380, 410, 967, 997, 2238, 2268
Bsc4I CCNNNNNNNGG 4 cut(s) 802, 1082, 1650, 1803
Bse1I ACTGG 4 cut(s) 1144, 1229, 1340, 2226
Bse21I CCTNAGG 1 cut(s) 384
Bse8I GATNNNNATC 1 cut(s) 2022
BseAI TCCGGA 1 cut(s) 1912
BseBI CCWGG 2 cut(s) 1162, 1729
BseDI CCNNGG 3 cut(s) 252, 528, 1161
BseGI GGATG 6 cut(s) 397, 538, 909, 1246, 1891, 2091
BseJI GATNNNNATC 1 cut(s) 2022
BseLI CCNNNNNNNGG 4 cut(s) 802, 1082, 1650, 1803
BseMII CTCAG 8 cut(s) 218, 375, 408, 537, 1043, 1106, 1392, 1641
BseNI ACTGG 4 cut(s) 1144, 1229, 1340, 2226
BseX3I CGGCCG 1 cut(s) 2029
BseXI GCAGC 5 cut(s) 148, 221, 1360, 1704, 2362
BsgI GTGCAG 1 cut(s) 905
Bsh1236I CGCG 3 cut(s) 130, 1207, 2028
Bsh1285I CGRYCG 2 cut(s) 2026, 2032
BshFI GGCC 7 cut(s) 79, 795, 1185, 1569, 1740, 2031, 2407
BshNI GGYRCC 1 cut(s) 562
BsiEI CGRYCG 2 cut(s) 2026, 2032
BsiSI CCGG 1 cut(s) 1913
BslFI GGGAC 4 cut(s) 216, 1262, 1558, 1971
BslI CCNNNNNNNGG 4 cut(s) 802, 1082, 1650, 1803
BsmAI GTCTC 7 cut(s) 34, 1131, 1189, 1483, 1613, 1664, 1744
BsmBI CGTCTC 1 cut(s) 1744
BsmFI GGGAC 4 cut(s) 216, 1262, 1558, 1971
BsmI GAATGC 2 cut(s) 1590, 2212
BsnI GGCC 7 cut(s) 79, 795, 1185, 1569, 1740, 2031, 2407
Bsp119I TTCGAA 1 cut(s) 2216
Bsp13I TCCGGA 1 cut(s) 1912
Bsp143I GATC 9 cut(s) 25, 145, 1132, 1202, 1318, 1379, 1786, 1888, 2023
BspACI CCGC 6 cut(s) 80, 431, 1207, 1820, 2028, 2084
BspANI GGCC 7 cut(s) 79, 795, 1185, 1569, 1740, 2031, 2407
BspCNI CTCAG 8 cut(s) 217, 376, 409, 536, 1044, 1105, 1393, 1640
BspEI TCCGGA 1 cut(s) 1912
BspFNI CGCG 3 cut(s) 130, 1207, 2028
BspLI GGNNCC 6 cut(s) 564, 1174, 1214, 1277, 1278, 1296
BspMAI CTGCAG 1 cut(s) 1567
BspMI ACCTGC 2 cut(s) 468, 1244
BspOI GCTAGC 1 cut(s) 765
BspPI GGATC 2 cut(s) 1127, 1210
BspT104I TTCGAA 1 cut(s) 2216
BspT107I GGYRCC 1 cut(s) 562
BsrI ACTGG 4 cut(s) 1144, 1229, 1340, 2226
BssECI CCNNGG 3 cut(s) 252, 528, 1161
BssMI GATC 9 cut(s) 25, 145, 1132, 1202, 1318, 1379, 1786, 1888, 2023
BssSI CACGAG 2 cut(s) 121, 1758
BssT1I CCWWGG 2 cut(s) 252, 528
Bst2BI CACGAG 2 cut(s) 121, 1758
Bst2UI CCWGG 2 cut(s) 1162, 1729
Bst4CI ACNGT 8 cut(s) 45, 310, 316, 337, 519, 578, 1125, 1507
Bst6I CTCTTC 3 cut(s) 1039, 1574, 1798
BstBI TTCGAA 1 cut(s) 2216
BstC8I GCNNGC 6 cut(s) 77, 636, 763, 767, 1567, 2336
BstENI CCTNNNNNAGG 1 cut(s) 1801
BstF5I GGATG 6 cut(s) 397, 538, 909, 1246, 1891, 2091
BstFNI CGCG 3 cut(s) 130, 1207, 2028
BstH2I RGCGCY 2 cut(s) 1698, 1836
BstHHI GCGC 6 cut(s) 130, 873, 994, 1697, 1835, 2182
BstKTI GATC 9 cut(s) 28, 148, 1135, 1205, 1321, 1382, 1789, 1891, 2026
BstMAI GTCTC 7 cut(s) 34, 1131, 1189, 1483, 1613, 1664, 1744
BstMBI GATC 9 cut(s) 25, 145, 1132, 1202, 1318, 1379, 1786, 1888, 2023
BstMCI CGRYCG 2 cut(s) 2026, 2032
BstMWI GCNNNNNNNGC 7 cut(s) 533, 1594, 2109, 2152, 2200, 2209, 2317
BstNI CCWGG 2 cut(s) 1162, 1729
BstNSI RCATGY 3 cut(s) 376, 638, 2143
BstSCI CCNGG 2 cut(s) 1160, 1727
BstSFI CTRYAG 4 cut(s) 987, 1563, 1861, 1894
BstUI CGCG 3 cut(s) 130, 1207, 2028
BstV1I GCAGC 5 cut(s) 148, 221, 1360, 1704, 2362
BstV2I GAAGAC 1 cut(s) 1551
BstX2I RGATCY 3 cut(s) 145, 1132, 1379
BstYI RGATCY 3 cut(s) 145, 1132, 1379
BstZI CGGCCG 1 cut(s) 2029
Bsu36I CCTNAGG 1 cut(s) 384
BsuRI GGCC 7 cut(s) 79, 795, 1185, 1569, 1740, 2031, 2407
BtrI CACGTC 1 cut(s) 1659
BtsCI GGATG 6 cut(s) 397, 538, 909, 1246, 1891, 2091
BtsI GCAGTG 2 cut(s) 829, 2391
BtsIMutI CAGTG 7 cut(s) 321, 398, 583, 697, 829, 1236, 2391
BveI ACCTGC 2 cut(s) 468, 1244
Cac8I GCNNGC 6 cut(s) 77, 636, 763, 767, 1567, 2336
CfoI GCGC 6 cut(s) 130, 873, 994, 1697, 1835, 2182
Cfr13I GGNCC 4 cut(s) 457, 1183, 1276, 1430
CseI GACGC 1 cut(s) 2340
Csp6I GTAC 7 cut(s) 370, 520, 563, 784, 1029, 1635, 1755
CspCI CAANNNNNGTGG 4 cut(s) 84, 119, 1522, 1557
CviAII CATG 9 cut(s) 373, 599, 635, 1169, 1259, 1427, 2087, 2140, 2177
CviQI GTAC 7 cut(s) 370, 520, 563, 784, 1029, 1635, 1755
DpnI GATC 9 cut(s) 27, 147, 1134, 1204, 1320, 1381, 1788, 1890, 2025
DpnII GATC 9 cut(s) 25, 145, 1132, 1202, 1318, 1379, 1786, 1888, 2023
EaeI YGGCCR 4 cut(s) 77, 793, 2029, 2405
EagI CGGCCG 1 cut(s) 2029
Eam1104I CTCTTC 3 cut(s) 1039, 1574, 1798
EarI CTCTTC 3 cut(s) 1039, 1574, 1798
EclXI CGGCCG 1 cut(s) 2029
Eco130I CCWWGG 2 cut(s) 252, 528
Eco147I AGGCCT 1 cut(s) 1740
Eco32I GATATC 1 cut(s) 1910
Eco47I GGWCC 3 cut(s) 457, 1276, 1430
Eco47III AGCGCT 2 cut(s) 1696, 1834
Eco52I CGGCCG 1 cut(s) 2029
Eco57I CTGAAG 6 cut(s) 186, 422, 431, 530, 1401, 1701
Eco81I CCTNAGG 1 cut(s) 384
EcoNI CCTNNNNNAGG 1 cut(s) 1801
EcoO109I RGGNCCY 2 cut(s) 1183, 1276
EcoRI GAATTC 3 cut(s) 271, 289, 910
EcoRII CCWGG 2 cut(s) 1160, 1727
EcoRV GATATC 1 cut(s) 1910
EcoT14I CCWWGG 2 cut(s) 252, 528
ErhI CCWWGG 2 cut(s) 252, 528
Esp3I CGTCTC 1 cut(s) 1744
FaeI CATG 9 cut(s) 376, 602, 638, 1172, 1262, 1430, 2090, 2143, 2180
FaqI GGGAC 4 cut(s) 216, 1262, 1558, 1971
FatI CATG 9 cut(s) 372, 598, 634, 1168, 1258, 1426, 2086, 2139, 2176
FauI CCCGC 1 cut(s) 2091
FauNDI CATATG 1 cut(s) 1770
FbaI TGATCA 1 cut(s) 1318
FblI GTMKAC 1 cut(s) 1655
Fnu4HI GCNGC 8 cut(s) 80, 162, 210, 1374, 1693, 1821, 2029, 2351
FokI GGATG 6 cut(s) 404, 545, 916, 1253, 1898, 2078
Fsp4HI GCNGC 8 cut(s) 80, 162, 210, 1374, 1693, 1821, 2029, 2351
FspI TGCGCA 1 cut(s) 872
GlaI GCGC 6 cut(s) 129, 872, 993, 1696, 1834, 2181
GluI GCNGC 8 cut(s) 80, 162, 210, 1374, 1693, 1821, 2029, 2351
GsuI CTGGAG 2 cut(s) 1323, 1750
HaeII RGCGCY 2 cut(s) 1698, 1836
HaeIII GGCC 7 cut(s) 79, 795, 1185, 1569, 1740, 2031, 2407
HapII CCGG 1 cut(s) 1913
HgaI GACGC 1 cut(s) 2340
HhaI GCGC 6 cut(s) 130, 873, 994, 1697, 1835, 2182
Hin1II CATG 9 cut(s) 376, 602, 638, 1172, 1262, 1430, 2090, 2143, 2180
Hin6I GCGC 6 cut(s) 128, 871, 992, 1695, 1833, 2180
HinP1I GCGC 6 cut(s) 128, 871, 992, 1695, 1833, 2180
HincII GTYRAC 2 cut(s) 1226, 1656
HindII GTYRAC 2 cut(s) 1226, 1656
HindIII AAGCTT 2 cut(s) 111, 2411
HpaII CCGG 1 cut(s) 1913
HphI GGTGA 6 cut(s) 1796, 1961, 1970, 1976, 1982, 2137
Hpy166II GTNNAC 4 cut(s) 669, 1226, 1656, 1847
Hpy8I GTNNAC 4 cut(s) 669, 1226, 1656, 1847
Hpy99I CGWCG 2 cut(s) 1660, 2334
HpyAV CCTTC 4 cut(s) 65, 161, 981, 2183
HpyCH4III ACNGT 8 cut(s) 45, 310, 316, 337, 519, 578, 1125, 1507
HpyCH4IV ACGT 3 cut(s) 1305, 1658, 1753
HpyF10VI GCNNNNNNNGC 7 cut(s) 533, 1594, 2109, 2152, 2200, 2209, 2317
HpySE526I ACGT 3 cut(s) 1305, 1658, 1753
Hsp92II CATG 9 cut(s) 376, 602, 638, 1172, 1262, 1430, 2090, 2143, 2180
HspAI GCGC 6 cut(s) 128, 871, 992, 1695, 1833, 2180
KflI GGGWCCC 1 cut(s) 1276
Kpn2I TCCGGA 1 cut(s) 1912
KpnI GGTACC 1 cut(s) 566
Ksp22I TGATCA 1 cut(s) 1318
Kzo9I GATC 9 cut(s) 25, 145, 1132, 1202, 1318, 1379, 1786, 1888, 2023
LmnI GCTCC 4 cut(s) 385, 1018, 1300, 2109
Lsp1109I GCAGC 5 cut(s) 148, 221, 1360, 1704, 2362
LweI GCATC 5 cut(s) 148, 523, 916, 2100, 2337
MaeII ACGT 3 cut(s) 1305, 1658, 1753
MaeIII GTNAC 5 cut(s) 310, 396, 953, 1477, 2228
MalI GATC 9 cut(s) 27, 147, 1134, 1204, 1320, 1381, 1788, 1890, 2025
MboI GATC 9 cut(s) 25, 145, 1132, 1202, 1318, 1379, 1786, 1888, 2023
MfeI CAATTG 1 cut(s) 134
MflI RGATCY 3 cut(s) 145, 1132, 1379
MlsI TGGCCA 1 cut(s) 795
MluNI TGGCCA 1 cut(s) 795
MmeI TCCRAC 6 cut(s) 773, 1160, 1402, 1584, 1906, 2166
Mox20I TGGCCA 1 cut(s) 795
MroI TCCGGA 1 cut(s) 1912
MroXI GAANNNNTTC 2 cut(s) 358, 914
MscI TGGCCA 1 cut(s) 795
MseI TTAA 3 cut(s) 140, 1308, 2415
MslI CAYNNNNRTG 4 cut(s) 618, 1263, 1995, 2175
Msp20I TGGCCA 1 cut(s) 795
MspA1I CMGCKG 1 cut(s) 212
MspI CCGG 1 cut(s) 1913
MspR9I CCNGG 2 cut(s) 1162, 1729
MunI CAATTG 1 cut(s) 134
Mva1269I GAATGC 2 cut(s) 1590, 2212
MvaI CCWGG 2 cut(s) 1162, 1729
MvnI CGCG 3 cut(s) 130, 1207, 2028
MwoI GCNNNNNNNGC 7 cut(s) 533, 1594, 2109, 2152, 2200, 2209, 2317
NdeI CATATG 1 cut(s) 1770
NdeII GATC 9 cut(s) 25, 145, 1132, 1202, 1318, 1379, 1786, 1888, 2023
NheI GCTAGC 1 cut(s) 761
NlaIII CATG 9 cut(s) 376, 602, 638, 1172, 1262, 1430, 2090, 2143, 2180
NlaIV GGNNCC 6 cut(s) 564, 1174, 1214, 1277, 1278, 1296
NmuCI GTSAC 3 cut(s) 396, 953, 1477
NsbI TGCGCA 1 cut(s) 872
NspI RCATGY 3 cut(s) 376, 638, 2143
NspV TTCGAA 1 cut(s) 2216
PaeI GCATGC 1 cut(s) 638
PceI AGGCCT 1 cut(s) 1740
PciI ACATGT 2 cut(s) 372, 2139
PcsI WCGNNNNNNNCGW 1 cut(s) 2335
PctI GAATGC 2 cut(s) 1590, 2212
PdmI GAANNNNTTC 2 cut(s) 358, 914
PfeI GAWTC 7 cut(s) 4, 359, 489, 653, 1640, 1724, 2388
PflFI GACNNNGTC 1 cut(s) 554
PfoI TCCNGGA 1 cut(s) 1727
PkrI GCNGC 8 cut(s) 81, 163, 211, 1375, 1694, 1822, 2030, 2352
Ple19I CGATCG 1 cut(s) 2026
PpuMI RGGWCCY 1 cut(s) 1276
PscI ACATGT 2 cut(s) 372, 2139
Psp5II RGGWCCY 1 cut(s) 1276
Psp6I CCWGG 2 cut(s) 1160, 1727
PspGI CCWGG 2 cut(s) 1160, 1727
PspN4I GGNNCC 6 cut(s) 564, 1174, 1214, 1277, 1278, 1296
PspPI GGNCC 4 cut(s) 457, 1183, 1276, 1430
PspPPI RGGWCCY 1 cut(s) 1276
PstI CTGCAG 1 cut(s) 1567
PsuI RGATCY 3 cut(s) 145, 1132, 1379
PsyI GACNNNGTC 1 cut(s) 554
PvuI CGATCG 1 cut(s) 2026
PvuII CAGCTG 1 cut(s) 212
RsaI GTAC 7 cut(s) 371, 521, 564, 785, 1030, 1636, 1756
RsaNI GTAC 7 cut(s) 370, 520, 563, 784, 1029, 1635, 1755
RseI CAYNNNNRTG 4 cut(s) 618, 1263, 1995, 2175
SalI GTCGAC 1 cut(s) 1654
SaqAI TTAA 3 cut(s) 140, 1308, 2415
SatI GCNGC 8 cut(s) 80, 162, 210, 1374, 1693, 1821, 2029, 2351
Sau3AI GATC 9 cut(s) 25, 145, 1132, 1202, 1318, 1379, 1786, 1888, 2023
Sau96I GGNCC 4 cut(s) 457, 1183, 1276, 1430
ScaI AGTACT 2 cut(s) 521, 1030
ScrFI CCNGG 2 cut(s) 1162, 1729
SfaNI GCATC 5 cut(s) 148, 523, 916, 2100, 2337
SfcI CTRYAG 4 cut(s) 987, 1563, 1861, 1894
SfuI TTCGAA 1 cut(s) 2216
SinI GGWCC 3 cut(s) 457, 1276, 1430
SmiMI CAYNNNNRTG 4 cut(s) 618, 1263, 1995, 2175
SmlI CTYRAG 2 cut(s) 1128, 1943
SmoI CTYRAG 2 cut(s) 1128, 1943
SphI GCATGC 1 cut(s) 638
SseBI AGGCCT 1 cut(s) 1740
SsiI CCGC 6 cut(s) 80, 431, 1207, 1820, 2028, 2084
StuI AGGCCT 1 cut(s) 1740
StyD4I CCNGG 2 cut(s) 1160, 1727
StyI CCWWGG 2 cut(s) 252, 528
TaaI ACNGT 8 cut(s) 45, 310, 316, 337, 519, 578, 1125, 1507
TaiI ACGT 3 cut(s) 1308, 1661, 1756
TaqI TCGA 9 cut(s) 487, 558, 627, 855, 1655, 1735, 1808, 2216, 2329
TatI WGTACW 4 cut(s) 369, 519, 1028, 1634
TauI GCSGC 3 cut(s) 82, 1823, 2031
TfiI GAWTC 7 cut(s) 4, 359, 489, 653, 1640, 1724, 2388
Tru1I TTAA 3 cut(s) 140, 1308, 2415
Tru9I TTAA 3 cut(s) 140, 1308, 2415
TscAI CASTG 7 cut(s) 321, 405, 583, 697, 829, 1236, 2398
TseFI GTSAC 3 cut(s) 396, 953, 1477
TseI GCWGC 5 cut(s) 161, 209, 1373, 1692, 2350
Tsp45I GTSAC 3 cut(s) 396, 953, 1477
TspDTI ATGAA 7 cut(s) 17, 948, 951, 1542, 1722, 1757, 2165
TspGWI ACGGA 4 cut(s) 1864, 1912, 2016, 2068
TspRI CASTG 7 cut(s) 321, 405, 583, 697, 829, 1236, 2398
Tth111I GACNNNGTC 1 cut(s) 554
VpaK11BI GGWCC 3 cut(s) 457, 1276, 1430
XagI CCTNNNNNAGG 1 cut(s) 1801
XapI RAATTY 6 cut(s) 91, 271, 289, 539, 910, 1675
XbaI TCTAGA 1 cut(s) 151
XceI RCATGY 3 cut(s) 376, 638, 2143
XmiI GTMKAC 1 cut(s) 1655
XmnI GAANNNNTTC 2 cut(s) 358, 914
ZrmI AGTACT 2 cut(s) 521, 1030
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.