Rmu_sc0004915.1_g000015

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004915.1
OG0009441
Physical Location & Seq
Forward (+)
95141 .. 97204
2064 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004915.1_g000015.1.cds

Sequence Viewer

Length: 2064 bp
atgtttttgcagaccatagaagcagagtcagttgtccagacgacacccagctcttctaaactagagcaaccaaagccaatgcctaagttgcaatctcctctttcaccagatgcagtcaattcttctctaaatgttgtcctgagcaatgaaaaggattctaaaagcagtgtaatcacttcaaaaaaggatgagcagtatccagtgaagcgtcagagtgcaatttccgggtctaaagtaagcacctcatcagacgtacctcaatacaaaaagaaacggtgccgcgcctgcatagcaaaagaaatgaagaaacagtactcaaccaaggaggcaacgatgtccaatgagagagccgaggtaccaggcaaaaacagtggggatcaagatcagcaacgtgactggggcagtggcagtgtcagttgtgttgatgaccaagctaaaagattacaagaatccaatagcaagaccatagagagagagaactctattggtgagactcttccttcatcagtctctttaacaaccggagccggcaagcaagaagttgggtatatcccaattggtcaaatgaagaaaaagttagatgagggtaatgatttcgctaaagtggtaacaaatgcaatcagagaaattgagttctgcattttggctttgcagttgagttctgatttagcacattctgctatcgataatgatgttgatgaacatgttcccttcaagcttggcatttcagttagtcctgtagaacacagaaaagaagaggaggccgttaccagacaagagctgagtcatactcatatgggagagtctttattggggggagctggcacaagtcagctgtctcaaaagcatgggaagtttgctgaatctgtttctaggggacaaggctccccactggaaaactatatgaacagatatgaatctatgagtcgatcagcaagtcagaacaataataagatggcgagttatattaacgggctaagagtctcaccgattccaaatgaggtaaatatgaagcctcccctgcagttgaagacccttgctacccctgttcaaaaatccgagaccggtctagttctaaatcgaatagctaaaaacgggacttcgagatataattcgtccacggtatcttcagttggaggtgggcttggtagaaagtcgagtcagaggacaagaatcaagaatgaaaagagacatgatcaaacaatgatgaagccagtatttttagatgatcacaagccaagtaagagaacaaggacccataagatgtcttacgtaggctcagggaagaagatcatgctacgtccgcaactgtcggaggaggagactagcagctcaacttctcgtagttcgtcaatgtggacaagtaagcaaaacgaaagcagtgcaagtagctgtagcaattattccaaggaatcttggagtccaagaaatgatgttttttccgaaagtgaaagtaaggaatcttggagtccaagaaatgatgatttttccaaaagtgaaagtaactcgcaggagagtgtaagtagtagctgtcgctcgtcggaggagcatagaattagattgacagattctgtagagagtgactatagccgtaaaaaatatccggagaaatctattggaaggttaagaaagatcaagaacaggttgggactcatctttcaccaccatcaccaccatcatcatcaccatcaccatcatggcaatgatcgttctacgtggaagaaggtacagaaaatgtttcgccacaaagacaagcatggcactaatgatcatggtcatggagaaggggtcaaaaaatcactagcagtgaaaaacatgtcaaataagaaccagggtgggcattttcatgcactcgtgaaagggctgatgagacatgttaagcattcaaagaagtcgaaaggtgggatcagacctcctcagcctcgtggccccaataagaagaaggttgtgaacaagttgcattggtggcagacggttcgacgtggtggagtaaagctgcctaaaagagggcgtgtaaaagtaggatttgccaataaaagaccaaaactacaaatgaagagatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0007275 GO:0008150 GO:0009555 GO:0009566 GO:0009567 GO:0019953 GO:0022414 GO:0032501 GO:0032502 GO:0044703 GO:0048229 GO:0048856 GO:0051704
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

687

Amino Acids

76.94

Weight (kDa)

9.95

Isoelectric Point (pI)

56.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 355
AccB1I GGYRCC 2 cut(s) 276, 355
AccII CGCG 1 cut(s) 282
AccIII TCCGGA 1 cut(s) 1603
AciI CCGC 2 cut(s) 280, 1304
AclWI GGATC 2 cut(s) 384, 1913
AcuI CTGAAG 1 cut(s) 1113
AfaI GTAC 4 cut(s) 255, 314, 357, 1728
AfiI CCNNNNNNNGG 1 cut(s) 2006
AflIII ACRYGT 3 cut(s) 703, 1815, 1873
AgeI ACCGGT 1 cut(s) 1064
AgsI TTSAA 5 cut(s) 180, 715, 1030, 1052, 1887
AjiI CACGTC 1 cut(s) 1982
AjnI CCWGG 2 cut(s) 358, 1830
Alw26I GTCTC 8 cut(s) 485, 514, 843, 988, 1055, 1183, 1316, 1864
AlwI GGATC 2 cut(s) 384, 1913
AlwNI CAGNNNCTG 1 cut(s) 1571
Aor13HI TCCGGA 1 cut(s) 1603
AoxI GGCC 2 cut(s) 762, 1927
ApeKI GCWGC 2 cut(s) 1329, 1996
AsiGI ACCGGT 1 cut(s) 1064
Asp700I GAANNNNTTC 1 cut(s) 705
Asp718I GGTACC 1 cut(s) 355
AspLEI GCGC 1 cut(s) 284
AspS9I GGNCC 2 cut(s) 1254, 1928
AsuC2I CCSGG 1 cut(s) 226
AsuHPI GGTGA 7 cut(s) 96, 500, 978, 1652, 1661, 1676, 1682
AvaII GGWCC 1 cut(s) 1254
BanI GGYRCC 2 cut(s) 276, 355
BarI GAAGNNNNNNTAC 2 cut(s) 296, 328
BauI CACGAG 2 cut(s) 1853, 1923
BbsI GAAGAC 1 cut(s) 1037
BbvCI CCTCAGC 1 cut(s) 1917
BbvI GCAGC 2 cut(s) 1341, 1983
BccI CCATC 5 cut(s) 949, 1674, 1683, 1695, 1701
BceAI ACGGC 2 cut(s) 749, 1575
BcgI CGANNNNNNTGC 4 cut(s) 1364, 1398, 1958, 1992
BciT130I CCWGG 2 cut(s) 360, 1832
BciVI GTATCC 1 cut(s) 207
BclI TGATCA 3 cut(s) 1195, 1228, 1768
BcnI CCSGG 1 cut(s) 226
BcoDI GTCTC 8 cut(s) 485, 514, 843, 988, 1055, 1183, 1316, 1864
BfaI CTAG 5 cut(s) 62, 873, 1070, 1326, 1802
BfmI CTRYAG 5 cut(s) 738, 1022, 1393, 1572, 1585
BfuI GTATCC 1 cut(s) 207
BisI GCNGC 3 cut(s) 280, 1330, 1997
BlsI GCNGC 3 cut(s) 281, 1331, 1998
BmcAI AGTACT 1 cut(s) 314
Bme1390I CCNGG 3 cut(s) 226, 360, 1832
Bme18I GGWCC 1 cut(s) 1254
BmgBI CACGTC 1 cut(s) 1982
BmgT120I GGNCC 2 cut(s) 1254, 1928
BmiI GGNNCC 6 cut(s) 278, 357, 526, 886, 1256, 1930
BmrFI CCNGG 3 cut(s) 226, 360, 1832
BmrI ACTGGG 1 cut(s) 406
BmsI GCATC 1 cut(s) 100
BmuI ACTGGG 1 cut(s) 406
BpiI GAAGAC 1 cut(s) 1037
BplI GAGNNNNNCTC 2 cut(s) 775, 807
Bpu10I CCTNAGC 3 cut(s) 140, 1279, 1917
BpuMI CCSGG 1 cut(s) 226
Bsa29I ATCGAT 1 cut(s) 684
BsaAI YACGTR 2 cut(s) 1273, 1716
BsaBI GATNNNNATC 2 cut(s) 381, 916
BsaI GGTCTC 1 cut(s) 1055
BsaJI CCNNGG 5 cut(s) 321, 351, 1119, 1407, 1831
BsaWI WCCGGW 3 cut(s) 521, 1064, 1603
BsaXI ACNNNNNCTCC 2 cut(s) 1773, 1803
Bsc4I CCNNNNNNNGG 1 cut(s) 2006
Bse118I RCCGGY 2 cut(s) 527, 1064
Bse1I ACTGG 4 cut(s) 200, 401, 897, 1214
Bse3DI GCAATG 2 cut(s) 151, 1708
Bse8I GATNNNNATC 2 cut(s) 381, 916
BseAI TCCGGA 1 cut(s) 1603
BseBI CCWGG 2 cut(s) 360, 1832
BseCI ATCGAT 1 cut(s) 684
BseDI CCNNGG 5 cut(s) 321, 351, 1119, 1407, 1831
BseGI GGATG 1 cut(s) 193
BseJI GATNNNNATC 2 cut(s) 381, 916
BseLI CCNNNNNNNGG 1 cut(s) 2006
BseMI GCAATG 2 cut(s) 151, 1708
BseMII CTCAG 4 cut(s) 131, 773, 1293, 1931
BseNI ACTGG 4 cut(s) 200, 401, 897, 1214
BseRI GAGGAG 6 cut(s) 87, 773, 1331, 1334, 1559, 1905
BseXI GCAGC 2 cut(s) 1341, 1983
BseYI CCCAGC 1 cut(s) 47
Bsh1236I CGCG 1 cut(s) 282
BshFI GGCC 2 cut(s) 764, 1929
BshNI GGYRCC 2 cut(s) 276, 355
BshTI ACCGGT 1 cut(s) 1064
BshVI ATCGAT 1 cut(s) 684
BsiSI CCGG 5 cut(s) 225, 522, 528, 1065, 1604
BslFI GGGAC 3 cut(s) 891, 1111, 1662
BslI CCNNNNNNNGG 1 cut(s) 2006
BsmAI GTCTC 8 cut(s) 485, 514, 843, 988, 1055, 1183, 1316, 1864
BsmFI GGGAC 3 cut(s) 891, 1111, 1662
BsmI GAATGC 1 cut(s) 1882
BsnI GGCC 2 cut(s) 764, 1929
Bso31I GGTCTC 1 cut(s) 1055
Bsp13I TCCGGA 1 cut(s) 1603
BspACI CCGC 2 cut(s) 280, 1304
BspANI GGCC 2 cut(s) 764, 1929
BspCNI CTCAG 4 cut(s) 132, 774, 1292, 1930
BspDI ATCGAT 1 cut(s) 684
BspEI TCCGGA 1 cut(s) 1603
BspFNI CGCG 1 cut(s) 282
BspLI GGNNCC 6 cut(s) 278, 357, 526, 886, 1256, 1930
BspMAI CTGCAG 1 cut(s) 1026
BspPI GGATC 2 cut(s) 384, 1913
BspQI GCTCTTC 1 cut(s) 58
BspT107I GGYRCC 2 cut(s) 276, 355
BspTNI GGTCTC 1 cut(s) 1055
BsrDI GCAATG 2 cut(s) 151, 1708
BsrFI RCCGGY 2 cut(s) 527, 1064
BsrI ACTGG 4 cut(s) 200, 401, 897, 1214
BssAI RCCGGY 2 cut(s) 527, 1064
BssECI CCNNGG 5 cut(s) 321, 351, 1119, 1407, 1831
BssSI CACGAG 2 cut(s) 1853, 1923
BssT1I CCWWGG 2 cut(s) 321, 1407
Bst2BI CACGAG 2 cut(s) 1853, 1923
Bst2UI CCWGG 2 cut(s) 360, 1832
Bst4CI ACNGT 6 cut(s) 276, 312, 371, 1123, 1311, 1975
Bst6I CTCTTC 4 cut(s) 58, 501, 750, 2051
BstAPI GCANNNNNTGC 1 cut(s) 677
BstBAI YACGTR 2 cut(s) 1273, 1716
BstC8I GCNNGC 4 cut(s) 286, 529, 533, 823
BstDEI CTNAG 6 cut(s) 84, 140, 782, 977, 1279, 1917
BstDSI CCRYGG 1 cut(s) 1119
BstENI CCTNNNNNAGG 1 cut(s) 2004
BstF5I GGATG 1 cut(s) 193
BstFNI CGCG 1 cut(s) 282
BstHHI GCGC 1 cut(s) 284
BstMAI GTCTC 8 cut(s) 485, 514, 843, 988, 1055, 1183, 1316, 1864
BstMWI GCNNNNNNNGC 8 cut(s) 73, 88, 285, 290, 677, 1021, 1303, 1966
BstNI CCWGG 2 cut(s) 360, 1832
BstNSI RCATGY 3 cut(s) 707, 1819, 1877
BstSCI CCNGG 3 cut(s) 224, 358, 1830
BstSFI CTRYAG 5 cut(s) 738, 1022, 1393, 1572, 1585
BstSNI TACGTA 1 cut(s) 1273
BstUI CGCG 1 cut(s) 282
BstV1I GCAGC 2 cut(s) 1341, 1983
BstV2I GAAGAC 1 cut(s) 1037
Bsu15I ATCGAT 1 cut(s) 684
BsuI GTATCC 1 cut(s) 207
BsuRI GGCC 2 cut(s) 764, 1929
BsuTUI ATCGAT 1 cut(s) 684
BtgI CCRYGG 1 cut(s) 1119
BtrI CACGTC 1 cut(s) 1982
BtsCI GGATG 1 cut(s) 193
BtsI GCAGTG 5 cut(s) 172, 409, 415, 1387, 1812
BtsIMutI CAGTG 8 cut(s) 172, 207, 376, 409, 415, 890, 1387, 1812
Cac8I GCNNGC 4 cut(s) 286, 529, 533, 823
CaiI CAGNNNCTG 1 cut(s) 1571
CfoI GCGC 1 cut(s) 284
Cfr10I RCCGGY 2 cut(s) 527, 1064
Cfr13I GGNCC 2 cut(s) 1254, 1928
ClaI ATCGAT 1 cut(s) 684
CseI GACGC 1 cut(s) 197
Csp6I GTAC 4 cut(s) 254, 313, 356, 1727
CspAI ACCGGT 1 cut(s) 1064
CspCI CAANNNNNGTGG 2 cut(s) 352, 387
CviQI GTAC 4 cut(s) 254, 313, 356, 1727
DdeI CTNAG 6 cut(s) 84, 140, 782, 977, 1279, 1917
Eam1104I CTCTTC 4 cut(s) 58, 501, 750, 2051
EarI CTCTTC 4 cut(s) 58, 501, 750, 2051
Eco105I TACGTA 1 cut(s) 1273
Eco130I CCWWGG 2 cut(s) 321, 1407
Eco31I GGTCTC 1 cut(s) 1055
Eco47I GGWCC 1 cut(s) 1254
Eco57I CTGAAG 1 cut(s) 1113
EcoNI CCTNNNNNAGG 1 cut(s) 2004
EcoO109I RGGNCCY 1 cut(s) 1254
EcoRII CCWGG 2 cut(s) 358, 1830
EcoT14I CCWWGG 2 cut(s) 321, 1407
ErhI CCWWGG 2 cut(s) 321, 1407
FaqI GGGAC 3 cut(s) 891, 1111, 1662
FauNDI CATATG 1 cut(s) 795
FbaI TGATCA 3 cut(s) 1195, 1228, 1768
Fnu4HI GCNGC 3 cut(s) 280, 1330, 1997
FokI GGATG 1 cut(s) 200
Fsp4HI GCNGC 3 cut(s) 280, 1330, 1997
FspBI CTAG 5 cut(s) 62, 873, 1070, 1326, 1802
GlaI GCGC 1 cut(s) 283
GluI GCNGC 3 cut(s) 280, 1330, 1997
GsaI CCCAGC 1 cut(s) 51
HaeIII GGCC 2 cut(s) 764, 1929
HapII CCGG 5 cut(s) 225, 522, 528, 1065, 1604
HgaI GACGC 1 cut(s) 197
HhaI GCGC 1 cut(s) 284
Hin6I GCGC 1 cut(s) 282
HinP1I GCGC 1 cut(s) 282
HindIII AAGCTT 1 cut(s) 716
HpaII CCGG 5 cut(s) 225, 522, 528, 1065, 1604
HphI GGTGA 7 cut(s) 96, 500, 978, 1652, 1661, 1676, 1682
Hpy166II GTNNAC 3 cut(s) 1119, 1359, 1951
Hpy188III TCNNGA 8 cut(s) 37, 139, 380, 1104, 1177, 1604, 1636, 1855
Hpy8I GTNNAC 3 cut(s) 1119, 1359, 1951
Hpy99I CGWCG 2 cut(s) 1543, 1983
HpyAV CCTTC 6 cut(s) 510, 721, 1614, 1717, 1778, 1936
HpyCH4III ACNGT 6 cut(s) 276, 312, 371, 1123, 1311, 1975
HpyCH4IV ACGT 6 cut(s) 252, 391, 1272, 1300, 1715, 1981
HpyF10VI GCNNNNNNNGC 8 cut(s) 73, 88, 285, 290, 677, 1021, 1303, 1966
HpyF3I CTNAG 6 cut(s) 84, 140, 782, 977, 1279, 1917
HpySE526I ACGT 6 cut(s) 252, 391, 1272, 1300, 1715, 1981
HspAI GCGC 1 cut(s) 282
Kpn2I TCCGGA 1 cut(s) 1603
KpnI GGTACC 1 cut(s) 359
KroI GCCGGC 1 cut(s) 527
KroNI GCCGGC 1 cut(s) 529
Ksp22I TGATCA 3 cut(s) 1195, 1228, 1768
LguI GCTCTTC 1 cut(s) 58
LmnI GCTCC 4 cut(s) 524, 818, 890, 1546
Lsp1109I GCAGC 2 cut(s) 1341, 1983
LweI GCATC 1 cut(s) 100
MaeI CTAG 5 cut(s) 62, 873, 1070, 1326, 1802
MaeII ACGT 6 cut(s) 252, 391, 1272, 1300, 1715, 1981
MaeIII GTNAC 5 cut(s) 392, 607, 766, 1502, 1580
MfeI CAATTG 1 cut(s) 555
MluCI AATT 7 cut(s) 118, 219, 555, 627, 1111, 1399, 1554
MmeI TCCRAC 3 cut(s) 1114, 1293, 1521
MroI TCCGGA 1 cut(s) 1603
MroNI GCCGGC 1 cut(s) 527
MroXI GAANNNNTTC 1 cut(s) 705
MseI TTAA 4 cut(s) 515, 969, 1625, 1878
MslI CAYNNNNRTG 4 cut(s) 1695, 1701, 1776, 1845
MspA1I CMGCKG 1 cut(s) 835
MspI CCGG 5 cut(s) 225, 522, 528, 1065, 1604
MspR9I CCNGG 3 cut(s) 226, 360, 1832
MunI CAATTG 1 cut(s) 555
Mva1269I GAATGC 1 cut(s) 1882
MvaI CCWGG 2 cut(s) 360, 1832
MvnI CGCG 1 cut(s) 282
MwoI GCNNNNNNNGC 8 cut(s) 73, 88, 285, 290, 677, 1021, 1303, 1966
NaeI GCCGGC 1 cut(s) 529
NciI CCSGG 1 cut(s) 226
NdeI CATATG 1 cut(s) 795
NgoMIV GCCGGC 1 cut(s) 527
NlaIV GGNNCC 6 cut(s) 278, 357, 526, 886, 1256, 1930
NmeAIII GCCGAG 1 cut(s) 376
NmuCI GTSAC 2 cut(s) 392, 1580
NspI RCATGY 3 cut(s) 707, 1819, 1877
PciI ACATGT 3 cut(s) 703, 1815, 1873
PciSI GCTCTTC 1 cut(s) 58
PctI GAATGC 1 cut(s) 1882
PdiI GCCGGC 1 cut(s) 529
PdmI GAANNNNTTC 1 cut(s) 705
PfeI GAWTC 9 cut(s) 155, 449, 863, 917, 991, 1173, 1412, 1460, 1568
PinAI ACCGGT 1 cut(s) 1064
PkrI GCNGC 3 cut(s) 281, 1331, 1998
Ppu21I YACGTR 2 cut(s) 1273, 1716
PpuMI RGGWCCY 1 cut(s) 1254
PscI ACATGT 3 cut(s) 703, 1815, 1873
Psp5II RGGWCCY 1 cut(s) 1254
Psp6I CCWGG 2 cut(s) 358, 1830
PspFI CCCAGC 1 cut(s) 47
PspGI CCWGG 2 cut(s) 358, 1830
PspN4I GGNNCC 6 cut(s) 278, 357, 526, 886, 1256, 1930
PspPI GGNCC 2 cut(s) 1254, 1928
PspPPI RGGWCCY 1 cut(s) 1254
PstI CTGCAG 1 cut(s) 1026
PstNI CAGNNNCTG 1 cut(s) 1571
PvuII CAGCTG 1 cut(s) 835
RsaI GTAC 4 cut(s) 255, 314, 357, 1728
RsaNI GTAC 4 cut(s) 254, 313, 356, 1727
RseI CAYNNNNRTG 4 cut(s) 1695, 1701, 1776, 1845
SapI GCTCTTC 1 cut(s) 58
SaqAI TTAA 4 cut(s) 515, 969, 1625, 1878
SatI GCNGC 3 cut(s) 280, 1330, 1997
Sau96I GGNCC 2 cut(s) 1254, 1928
ScaI AGTACT 1 cut(s) 314
ScrFI CCNGG 3 cut(s) 226, 360, 1832
SfaNI GCATC 1 cut(s) 100
SfcI CTRYAG 5 cut(s) 738, 1022, 1393, 1572, 1585
SinI GGWCC 1 cut(s) 1254
SmiMI CAYNNNNRTG 4 cut(s) 1695, 1701, 1776, 1845
SnaBI TACGTA 1 cut(s) 1273
Sse9I AATT 7 cut(s) 118, 219, 555, 627, 1111, 1399, 1554
SsiI CCGC 2 cut(s) 280, 1304
SspMI CTAG 5 cut(s) 62, 873, 1070, 1326, 1802
StyD4I CCNGG 3 cut(s) 224, 358, 1830
StyI CCWWGG 2 cut(s) 321, 1407
TaaI ACNGT 6 cut(s) 276, 312, 371, 1123, 1311, 1975
TaiI ACGT 6 cut(s) 255, 394, 1275, 1303, 1718, 1984
TaqI TCGA 7 cut(s) 684, 928, 1081, 1103, 1157, 1895, 1978
TasI AATT 7 cut(s) 118, 219, 555, 627, 1111, 1399, 1554
TatI WGTACW 1 cut(s) 312
TauI GCSGC 1 cut(s) 282
TfiI GAWTC 9 cut(s) 155, 449, 863, 917, 991, 1173, 1412, 1460, 1568
Tru1I TTAA 4 cut(s) 515, 969, 1625, 1878
Tru9I TTAA 4 cut(s) 515, 969, 1625, 1878
TscAI CASTG 8 cut(s) 172, 207, 376, 409, 415, 897, 1387, 1812
TseFI GTSAC 2 cut(s) 392, 1580
TseI GCWGC 2 cut(s) 1329, 1996
Tsp45I GTSAC 2 cut(s) 392, 1580
TspRI CASTG 8 cut(s) 172, 207, 376, 409, 415, 897, 1387, 1812
VpaK11BI GGWCC 1 cut(s) 1254
XagI CCTNNNNNAGG 1 cut(s) 2004
XceI RCATGY 3 cut(s) 707, 1819, 1877
XcmI CCANNNNNNNNNTGG 1 cut(s) 1694
XmnI GAANNNNTTC 1 cut(s) 705
XspI CTAG 5 cut(s) 62, 873, 1070, 1326, 1802
ZrmI AGTACT 1 cut(s) 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.