FvH4_5g34270

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0009441
Physical Location & Seq
Forward (+)
24942528 .. 24944056
1529 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g34270.t1

Sequence Viewer

Length: 1101 bp
ATGGATCTTGTGGAAGTTTTGTCTAATCTCCATGCCTTGAGACAATTATATGAACTCCTTCAGAACATTGAAGACGATCTTCAACATATAAATTCAAAGAATGTACACCAAACTTCGGACCCGAGAGCGCGAAAACTCTTCAAACACATCTTGGATGATGCTACTATAGATGTTCCATACTCGTTCAAAGAAGCACAGTCAGTTGTCCACAAGACACCTTGCTCTTCTCAACTAGAGCAACCAAAGGCAATGCCCAAGTTACAGTCTCCTCTTTCACTAGATACTGTCAATGCTTCTCTAAATGTTGTCCTGAACAATGAAAACGATTCTAAGAGCAGCATGGTCACTTCAGAAAAGGATGAGCAGTATCCAGTGAAGAGCCAGAGTACAATATTGAGGTCTAAAGTAACCACCTCATCAGATGTGCCTCTATACAAAAAGAAAAAGTGTCGCGCCTGCATTGCAAAAGAAATGAAGAGACAGTACTCAACCAAGGGCGCAACAATGTCTAATGAGAGAGTCGAGGTACCAGGTAAAAACAGTGGGGATCCAGACCAGCAACGTGACTGGGGTAGTGGCAGTGTCAGTTTTATTGATGAGCAAGCTAAAATATTACCAGAATCAAATAGCAAGACCATAGAGAGAGATACCCCTATTGATGAAACTCTTCCTTCATCAGTCTCTCTAACAACCGGGGCTGGCAAGCAAGAAGTTGGGTATATCGTAGTTGGTGAAATGAAGAAAAACTTGGATGAGGGTGATGATTTGGCCAAAGTAGTAACAAATGCAATCAGACAAATTGAGTCCTGCATTTTGGCATTGCAGTTGAGTTCTGACTTGGCACATTCTTTTATCAATAATGTTGTTGATGAACATGTTCCCTTCAAGCTTAGCATTTCAATTAGTCTTGTAGAACACAGCAAAGAGGAGGCCATTATTAGACAAGAGATGAATCAGACTGATACGGGAGAGTCTTCATGGGGGATTGGCACACAGTTAACTCAATTTCCTCAAAAGCATGGGAAGTTAGCTGATTCTGTGTCTAGGGGACAAGGCTCCCCAGTGGAAAACTATATGAAGAGATTTGAGTCTCTGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0007275 GO:0008150 GO:0009555 GO:0009566 GO:0009567 GO:0019953 GO:0022414 GO:0032501 GO:0032502 GO:0044703 GO:0048229 GO:0048856 GO:0051704
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

367

Amino Acids

40.66

Weight (kDa)

5.68

Isoelectric Point (pI)

49.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 526
AccB1I GGYRCC 1 cut(s) 526
AccII CGCG 2 cut(s) 130, 453
AclWI GGATC 3 cut(s) 12, 542, 555
AcoI YGGCCR 1 cut(s) 768
AcsI RAATTY 1 cut(s) 91
AcuI CTGAAG 2 cut(s) 44, 333
AfaI GTAC 4 cut(s) 105, 388, 485, 528
AfiI CCNNNNNNNGG 1 cut(s) 115
AflIII ACRYGT 1 cut(s) 874
AgsI TTSAA 7 cut(s) 71, 83, 96, 142, 187, 886, 900
AjnI CCWGG 1 cut(s) 529
AjuI GAANNNNNNNTTGG 2 cut(s) 731, 763
AluBI AGCT 3 cut(s) 605, 889, 1031
AluI AGCT 3 cut(s) 605, 889, 1031
Alw26I GTCTC 5 cut(s) 34, 270, 472, 685, 1095
AlwI GGATC 3 cut(s) 12, 542, 555
Ama87I CYCGRG 1 cut(s) 121
AoxI GGCC 2 cut(s) 768, 930
ApeKI GCWGC 1 cut(s) 336
ApoI RAATTY 1 cut(s) 91
Asp700I GAANNNNTTC 3 cut(s) 57, 666, 876
Asp718I GGTACC 1 cut(s) 526
AspLEI GCGC 3 cut(s) 130, 455, 500
AspS9I GGNCC 1 cut(s) 118
AsuC2I CCSGG 1 cut(s) 694
AsuHPI GGTGA 2 cut(s) 743, 770
AvaI CYCGRG 1 cut(s) 121
AvaII GGWCC 1 cut(s) 118
BalI TGGCCA 1 cut(s) 770
BamHI GGATCC 1 cut(s) 547
BanI GGYRCC 1 cut(s) 526
BarI GAAGNNNNNNTAC 2 cut(s) 467, 499
BbsI GAAGAC 2 cut(s) 78, 966
BbvI GCAGC 1 cut(s) 348
BciT130I CCWGG 1 cut(s) 531
BciVI GTATCC 1 cut(s) 378
BcnI CCSGG 1 cut(s) 694
BcoDI GTCTC 5 cut(s) 34, 270, 472, 685, 1095
BfaI CTAG 3 cut(s) 233, 278, 1044
BfmI CTRYAG 1 cut(s) 165
BfuI GTATCC 1 cut(s) 378
BisI GCNGC 1 cut(s) 337
BlpI GCTNAGC 1 cut(s) 890
BlsI GCNGC 1 cut(s) 338
BmcAI AGTACT 1 cut(s) 485
Bme1390I CCNGG 2 cut(s) 531, 694
Bme18I GGWCC 1 cut(s) 118
BmeT110I CYCGRG 1 cut(s) 121
BmgT120I GGNCC 1 cut(s) 118
BmiI GGNNCC 4 cut(s) 120, 528, 549, 1057
BmrFI CCNGG 2 cut(s) 531, 694
BmrI ACTGGG 2 cut(s) 577, 1055
BmsI GCATC 1 cut(s) 148
BmuI ACTGGG 2 cut(s) 577, 1055
BpiI GAAGAC 2 cut(s) 78, 966
Bpu1102I GCTNAGC 1 cut(s) 890
BpuEI CTTGAG 1 cut(s) 58
BpuMI CCSGG 1 cut(s) 694
BsaJI CCNNGG 2 cut(s) 492, 693
Bsc4I CCNNNNNNNGG 1 cut(s) 115
Bse1I ACTGG 3 cut(s) 371, 572, 1061
Bse3DI GCAATG 3 cut(s) 255, 459, 818
BseBI CCWGG 1 cut(s) 531
BseDI CCNNGG 2 cut(s) 492, 693
BseGI GGATG 3 cut(s) 160, 364, 757
BseLI CCNNNNNNNGG 1 cut(s) 115
BseMI GCAATG 3 cut(s) 255, 459, 818
BseMII CTCAG 1 cut(s) 1085
BseNI ACTGG 3 cut(s) 371, 572, 1061
BseRI GAGGAG 2 cut(s) 258, 941
BseXI GCAGC 1 cut(s) 348
Bsh1236I CGCG 2 cut(s) 130, 453
BshFI GGCC 2 cut(s) 770, 932
BshNI GGYRCC 1 cut(s) 526
BsiHKCI CYCGRG 1 cut(s) 121
BsiSI CCGG 1 cut(s) 693
BslFI GGGAC 1 cut(s) 1062
BslI CCNNNNNNNGG 1 cut(s) 115
BsmAI GTCTC 5 cut(s) 34, 270, 472, 685, 1095
BsmFI GGGAC 1 cut(s) 1062
BsnI GGCC 2 cut(s) 770, 932
BsoBI CYCGRG 1 cut(s) 121
Bsp1407I TGTACA 1 cut(s) 103
Bsp143I GATC 3 cut(s) 4, 76, 547
Bsp1720I GCTNAGC 1 cut(s) 890
BspANI GGCC 2 cut(s) 770, 932
BspCNI CTCAG 1 cut(s) 1086
BspFNI CGCG 2 cut(s) 130, 453
BspLI GGNNCC 4 cut(s) 120, 528, 549, 1057
BspPI GGATC 3 cut(s) 12, 542, 555
BspQI GCTCTTC 2 cut(s) 229, 371
BspT107I GGYRCC 1 cut(s) 526
BsrDI GCAATG 3 cut(s) 255, 459, 818
BsrGI TGTACA 1 cut(s) 103
BsrI ACTGG 3 cut(s) 371, 572, 1061
BssECI CCNNGG 2 cut(s) 492, 693
BssMI GATC 3 cut(s) 4, 76, 547
BssT1I CCWWGG 1 cut(s) 492
Bst2UI CCWGG 1 cut(s) 531
Bst4CI ACNGT 6 cut(s) 198, 264, 286, 483, 542, 996
Bst6I CTCTTC 6 cut(s) 143, 229, 371, 470, 672, 1073
BstAUI TGTACA 1 cut(s) 103
BstC8I GCNNGC 4 cut(s) 457, 603, 700, 704
BstDEI CTNAG 3 cut(s) 330, 890, 1094
BstF5I GGATG 3 cut(s) 160, 364, 757
BstFNI CGCG 2 cut(s) 130, 453
BstHHI GCGC 3 cut(s) 130, 455, 500
BstKTI GATC 3 cut(s) 7, 79, 550
BstMAI GTCTC 5 cut(s) 34, 270, 472, 685, 1095
BstMBI GATC 3 cut(s) 4, 76, 547
BstMWI GCNNNNNNNGC 1 cut(s) 461
BstNI CCWGG 1 cut(s) 531
BstNSI RCATGY 1 cut(s) 878
BstSCI CCNGG 2 cut(s) 529, 692
BstSFI CTRYAG 1 cut(s) 165
BstUI CGCG 2 cut(s) 130, 453
BstV1I GCAGC 1 cut(s) 348
BstV2I GAAGAC 2 cut(s) 78, 966
BstX2I RGATCY 2 cut(s) 4, 547
BstYI RGATCY 2 cut(s) 4, 547
BsuI GTATCC 1 cut(s) 378
BsuRI GGCC 2 cut(s) 770, 932
BtsCI GGATG 3 cut(s) 160, 364, 757
BtsI GCAGTG 1 cut(s) 586
BtsIMutI CAGTG 4 cut(s) 378, 547, 586, 1068
Cac8I GCNNGC 4 cut(s) 457, 603, 700, 704
CfoI GCGC 3 cut(s) 130, 455, 500
Cfr13I GGNCC 1 cut(s) 118
CsiI ACCWGGT 1 cut(s) 529
Csp6I GTAC 4 cut(s) 104, 387, 484, 527
CviAII CATG 5 cut(s) 32, 340, 875, 978, 1019
CviJI RGCY 8 cut(s) 381, 605, 698, 770, 889, 932, 1031, 1056
CviKI_1 RGCY 8 cut(s) 381, 605, 698, 770, 889, 932, 1031, 1056
CviQI GTAC 4 cut(s) 104, 387, 484, 527
DdeI CTNAG 3 cut(s) 330, 890, 1094
DpnI GATC 3 cut(s) 6, 78, 549
DpnII GATC 3 cut(s) 4, 76, 547
EaeI YGGCCR 1 cut(s) 768
Eam1104I CTCTTC 6 cut(s) 143, 229, 371, 470, 672, 1073
EarI CTCTTC 6 cut(s) 143, 229, 371, 470, 672, 1073
Eco130I CCWWGG 1 cut(s) 492
Eco47I GGWCC 1 cut(s) 118
Eco57I CTGAAG 2 cut(s) 44, 333
Eco88I CYCGRG 1 cut(s) 121
EcoRII CCWGG 1 cut(s) 529
EcoT14I CCWWGG 1 cut(s) 492
ErhI CCWWGG 1 cut(s) 492
FaeI CATG 5 cut(s) 35, 343, 878, 981, 1022
FalI AAGNNNNNCTT 4 cut(s) 63, 95, 731, 763
FaqI GGGAC 1 cut(s) 1062
FatI CATG 5 cut(s) 31, 339, 874, 977, 1018
Fnu4HI GCNGC 1 cut(s) 337
FokI GGATG 3 cut(s) 167, 371, 764
Fsp4HI GCNGC 1 cut(s) 337
FspBI CTAG 3 cut(s) 233, 278, 1044
GlaI GCGC 3 cut(s) 129, 454, 499
GluI GCNGC 1 cut(s) 337
HaeIII GGCC 2 cut(s) 770, 932
HapII CCGG 1 cut(s) 693
HhaI GCGC 3 cut(s) 130, 455, 500
Hin1II CATG 5 cut(s) 35, 343, 878, 981, 1022
Hin6I GCGC 3 cut(s) 128, 453, 498
HinP1I GCGC 3 cut(s) 128, 453, 498
HincII GTYRAC 1 cut(s) 999
HindII GTYRAC 1 cut(s) 999
HindIII AAGCTT 1 cut(s) 887
HinfI GANTC 8 cut(s) 326, 519, 620, 803, 952, 971, 1034, 1088
HpaI GTTAAC 1 cut(s) 999
HpaII CCGG 1 cut(s) 693
HphI GGTGA 2 cut(s) 743, 770
Hpy166II GTNNAC 3 cut(s) 106, 208, 999
Hpy188I TCNGA 8 cut(s) 63, 118, 352, 421, 794, 835, 957, 1095
Hpy188III TCNNGA 2 cut(s) 310, 551
Hpy8I GTNNAC 3 cut(s) 106, 208, 999
HpyAV CCTTC 3 cut(s) 68, 681, 892
HpyCH4III ACNGT 6 cut(s) 198, 264, 286, 483, 542, 996
HpyCH4IV ACGT 1 cut(s) 562
HpyCH4V TGCA 5 cut(s) 459, 464, 788, 810, 823
HpyF10VI GCNNNNNNNGC 1 cut(s) 461
HpyF3I CTNAG 3 cut(s) 330, 890, 1094
HpySE526I ACGT 1 cut(s) 562
Hsp92II CATG 5 cut(s) 35, 343, 878, 981, 1022
HspAI GCGC 3 cut(s) 128, 453, 498
KpnI GGTACC 1 cut(s) 530
KspAI GTTAAC 1 cut(s) 999
Kzo9I GATC 3 cut(s) 4, 76, 547
LguI GCTCTTC 2 cut(s) 229, 371
LmnI GCTCC 1 cut(s) 1061
Lsp1109I GCAGC 1 cut(s) 348
LweI GCATC 1 cut(s) 148
MabI ACCWGGT 1 cut(s) 529
MaeI CTAG 3 cut(s) 233, 278, 1044
MaeII ACGT 1 cut(s) 562
MaeIII GTNAC 5 cut(s) 258, 343, 406, 563, 778
MalI GATC 3 cut(s) 6, 78, 549
MboI GATC 3 cut(s) 4, 76, 547
MflI RGATCY 2 cut(s) 4, 547
MlsI TGGCCA 1 cut(s) 770
MluCI AATT 5 cut(s) 44, 91, 798, 900, 1004
MluNI TGGCCA 1 cut(s) 770
MlyI GAGTC 4 cut(s) 528, 812, 980, 1097
MnlI CCTC 9 cut(s) 279, 390, 424, 438, 517, 748, 919, 922, 1020
Mox20I TGGCCA 1 cut(s) 770
MroXI GAANNNNTTC 3 cut(s) 57, 666, 876
MscI TGGCCA 1 cut(s) 770
MseI TTAA 1 cut(s) 998
Msp20I TGGCCA 1 cut(s) 770
MspI CCGG 1 cut(s) 693
MspR9I CCNGG 2 cut(s) 531, 694
MvaI CCWGG 1 cut(s) 531
MvnI CGCG 2 cut(s) 130, 453
MwoI GCNNNNNNNGC 1 cut(s) 461
NciI CCSGG 1 cut(s) 694
NdeII GATC 3 cut(s) 4, 76, 547
NlaIII CATG 5 cut(s) 35, 343, 878, 981, 1022
NlaIV GGNNCC 4 cut(s) 120, 528, 549, 1057
NmuCI GTSAC 2 cut(s) 343, 563
NspI RCATGY 1 cut(s) 878
PciI ACATGT 1 cut(s) 874
PciSI GCTCTTC 2 cut(s) 229, 371
PdmI GAANNNNTTC 3 cut(s) 57, 666, 876
PfeI GAWTC 4 cut(s) 326, 620, 952, 1034
PkrI GCNGC 1 cut(s) 338
PleI GAGTC 4 cut(s) 527, 811, 979, 1096
PpsI GAGTC 4 cut(s) 527, 811, 979, 1096
PscI ACATGT 1 cut(s) 874
Psp6I CCWGG 1 cut(s) 529
PspGI CCWGG 1 cut(s) 529
PspN4I GGNNCC 4 cut(s) 120, 528, 549, 1057
PspPI GGNCC 1 cut(s) 118
PsuI RGATCY 2 cut(s) 4, 547
RsaI GTAC 4 cut(s) 105, 388, 485, 528
RsaNI GTAC 4 cut(s) 104, 387, 484, 527
SapI GCTCTTC 2 cut(s) 229, 371
SaqAI TTAA 1 cut(s) 998
SatI GCNGC 1 cut(s) 337
Sau3AI GATC 3 cut(s) 4, 76, 547
Sau96I GGNCC 1 cut(s) 118
ScaI AGTACT 1 cut(s) 485
SchI GAGTC 4 cut(s) 528, 812, 980, 1097
ScrFI CCNGG 2 cut(s) 531, 694
SetI ASST 9 cut(s) 220, 401, 416, 528, 535, 565, 607, 891, 1033
SexAI ACCWGGT 1 cut(s) 529
SfaNI GCATC 1 cut(s) 148
SfcI CTRYAG 1 cut(s) 165
SinI GGWCC 1 cut(s) 118
SmlI CTYRAG 1 cut(s) 37
SmoI CTYRAG 1 cut(s) 37
Sse9I AATT 5 cut(s) 44, 91, 798, 900, 1004
SspI AATATT 2 cut(s) 393, 612
SspMI CTAG 3 cut(s) 233, 278, 1044
StyD4I CCNGG 2 cut(s) 529, 692
StyI CCWWGG 1 cut(s) 492
TaaI ACNGT 6 cut(s) 198, 264, 286, 483, 542, 996
TaiI ACGT 1 cut(s) 565
TaqI TCGA 1 cut(s) 522
TasI AATT 5 cut(s) 44, 91, 798, 900, 1004
TatI WGTACW 3 cut(s) 103, 386, 483
TfiI GAWTC 4 cut(s) 326, 620, 952, 1034
Tru1I TTAA 1 cut(s) 998
Tru9I TTAA 1 cut(s) 998
TscAI CASTG 4 cut(s) 378, 547, 586, 1068
TseFI GTSAC 2 cut(s) 343, 563
TseI GCWGC 1 cut(s) 336
Tsp45I GTSAC 2 cut(s) 343, 563
TspRI CASTG 4 cut(s) 378, 547, 586, 1068
VpaK11BI GGWCC 1 cut(s) 118
XapI RAATTY 1 cut(s) 91
XceI RCATGY 1 cut(s) 878
XmnI GAANNNNTTC 3 cut(s) 57, 666, 876
XspI CTAG 3 cut(s) 233, 278, 1044
ZrmI AGTACT 1 cut(s) 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.