FvH4_1g25520

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0009441
Physical Location & Seq
Reverse (-)
17276773 .. 17281888
5116 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g25520.t5

Sequence Viewer

Length: 2121 bp
ATGGATCTTGTGGAAGTTCTGTCTAGTCTCCATGCCTTGAGACAATTATATGAACTCCTTCAGAACATCGAAGATCATGATATTCAACATATAAATTCAAAGAATGTAACCCAAACTTTGGATCTGAGAGCGCGAAAACTCTTCAAGCACCTCTTGGATGATGCTACTGAGAAGATGTTCCATACTCATTCAAAGATAACAGAAGCACACTCTGTTGTCCACAAGACACCCTTGAATGCTTCTCTAAATGTTGTCCTGAACAATGAAAAGGATTCTAAGAGCAGCATGGTCACTTCAGAAAAGGATGAGCAGTATCCGGTGAAGAGCCAGAGTGCAATATCCGTGTCTAAAGTAACCACCTCATCAGATGTGCCTCTATACAAAAAGAAAAGGTGCCGCGCCTGCATTGCAAAAGAAATGAAGAGACAGTACTCAACCAAGGACGCAACGATGTCCAATGAGAGAGCCGAGGTACCAGACAAAAACAGTGGGGATCAAGATCAGCAACGTGACTGGGGTAGTGGCAGTATCAGTTGTGTTGATGAGCAAGCTAAAATATTACCAGAATCCAATAGCAAGACCATAGAGAGAGAGACTCCTATTAATGAAACTCTTCCTTCACCAGTCTCATTAACAACCGGGGCTGGCAAGCAAGAAGTTGTGTATGATTTCACCAAAGTAGTAACAAATGCAATCAGACAAATTGAGTCCTGCATTTTGGCATTGCAGTTGAGTTCTGACTTGACAAAATCTCCTATCAATAATGTTGCTGATGAACATGTTCCCTTCAAGCTTAGCATTTCAGTTAGTCCTGTCGAACACAGAAAAGAGGAGGCCATTATCAGACACGAGATGAATCAGACTGATATTGATACTGATACTGATACGGGAGAGCCTTCATGGGAGAAAGGCTCACAGTTGACTCAAACTTCTCAAAAACATGAGAAGACAGCTGATCCTGTTTCTAGGGGACAAGGCTCCCCGGTGGAAAGCTATATGAAGAGATGTGAGTCTCTGAGTCGATCAGCAAGTCAGAATATTAATAAGATGGTAAGTTATGTTAACGGGCAAAAAGTCTCACCAAATCCTAATGAAGTAACTATGAAGCCTCCCCTGCAGTTGAAGACAGTTGCTACTCCTGTTCAAACATCAGAAACCAGTCTAGACCAAAATCAAACAGGTAGAAGACAAACTTCGAGATATAATTCTTCCATGCCACCACAGTCAATGGGGAACGTGGTATCTGCAGTTGGAGGTGGGCTTGGTACAAGGTCAAGTCAGACGAGAAGACTCAAGACTGGAAAGAGACATGATCAAATAATGATGAGGCCAGTGTTCTTAGATGATGACATGCCAAGTCAGAGAACAAGGACATGTAAGATGTCAAATGTAAGCTCAAGGAAGAAGATCCTACCGCGTCAGGAAGAGTCAGAGGAGGAGAATAGCAGCTCCAGTTCTCGCAGTTCGTCAATGTGGTCGAGTAAGCAAAGTGAAAGCAGTGCAAGTAGCTGTACCAATTATTCTGAAGAATCTTGGAGTCCAAGACACGATGATTTTTCTGAAAGTGATATTAACTCACAGGACAGTTTAAGTAGTAGCTATCACTCATCAGAGGAGGAGGATAGAAGTAGATTGACAGATTCTGTAGAGAGTGACTACAGCCATAGAAAATATCCGGACAAATCTATCGGGAGGTTAAGAAAGATCAAAAACAAGTTGGGACTCATCTTTCACCACCATCACCACCATCATCATCACCATCACCATCATGGTAATGATCGGAAGAAGGTACAAAAAATGTTTCACCACAAAGACAAGTGTGGCAGGAATGATCATGGTCAAGCACGAGTCAAAAAATCACTAGCGGTGAAAAACATGTCAAATAAGAACCAGGGTGGGCAGTTTCATGCATTAGTGAAAGGGCTAATGAGGCATACTAAGCACTCGAAGAAGACGAAAGGTGGGATCAGACCTCATCAGCCTCATGGCCTCAATAAGAAGGTTGTGAACAAGCTGCATTGGTGGCAGACGGTTCGGCATGGTGGAGTAAAGCTGCCTAAAAGAGGGCGTGTAAAAGTAGGATTTGCCAATAAAAGACCAAAACTACCAATGAAGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0007275 GO:0008150 GO:0009555 GO:0009566 GO:0009567 GO:0019953 GO:0022414 GO:0032501 GO:0032502 GO:0044703 GO:0048229 GO:0048856 GO:0051704
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

707

Amino Acids

79.81

Weight (kDa)

9.57

Isoelectric Point (pI)

61.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 472
AccB1I GGYRCC 2 cut(s) 393, 472
AccB7I CCANNNNNTGG 1 cut(s) 118
AccII CGCG 3 cut(s) 133, 399, 1417
AccIII TCCGGA 1 cut(s) 1675
AciI CCGC 3 cut(s) 397, 1415, 1865
AclWI GGATC 6 cut(s) 12, 129, 501, 950, 1402, 1973
AcsI RAATTY 1 cut(s) 94
AcuI CTGAAG 3 cut(s) 44, 279, 1545
AfaI GTAC 5 cut(s) 431, 474, 1267, 1513, 1791
AfiI CCNNNNNNNGG 2 cut(s) 118, 2063
AflIII ACRYGT 3 cut(s) 778, 1373, 1875
AgsI TTSAA 8 cut(s) 86, 99, 145, 192, 235, 790, 1123, 1145
AjnI CCWGG 1 cut(s) 1890
Alw26I GTCTC 8 cut(s) 32, 34, 418, 587, 631, 1017, 1081, 1300
AlwI GGATC 6 cut(s) 12, 129, 501, 950, 1402, 1973
AlwNI CAGNNNCTG 1 cut(s) 1643
Aor13HI TCCGGA 1 cut(s) 1675
AoxI GGCC 3 cut(s) 834, 1328, 1987
ApeKI GCWGC 4 cut(s) 282, 1446, 2014, 2053
ApoI RAATTY 1 cut(s) 94
AseI ATTAAT 2 cut(s) 603, 1041
Asp700I GAANNNNTTC 4 cut(s) 57, 176, 612, 780
Asp718I GGTACC 1 cut(s) 472
AspLEI GCGC 2 cut(s) 133, 401
AsuC2I CCSGG 2 cut(s) 640, 983
BanI GGYRCC 2 cut(s) 393, 472
BarI GAAGNNNNNNTAC 2 cut(s) 413, 445
BauI CACGAG 2 cut(s) 848, 1845
BbsI GAAGAC 5 cut(s) 953, 1130, 1192, 1294, 1958
BbvI GCAGC 4 cut(s) 294, 1458, 2001, 2040
BccI CCATC 5 cut(s) 1042, 1746, 1755, 1767, 1773
BcgI CGANNNNNNTGC 2 cut(s) 2015, 2049
BciT130I CCWGG 1 cut(s) 1892
BciVI GTATCC 1 cut(s) 324
BclI TGATCA 2 cut(s) 1312, 1831
BcnI CCSGG 2 cut(s) 640, 983
BcoDI GTCTC 8 cut(s) 32, 34, 418, 587, 631, 1017, 1081, 1300
BfaI CTAG 4 cut(s) 24, 966, 1163, 1862
BfmI CTRYAG 4 cut(s) 1115, 1245, 1644, 1657
BfuI GTATCC 1 cut(s) 324
BisI GCNGC 5 cut(s) 283, 397, 1447, 2015, 2054
BlpI GCTNAGC 1 cut(s) 794
BlsI GCNGC 5 cut(s) 284, 398, 1448, 2016, 2055
BmcAI AGTACT 1 cut(s) 431
Bme1390I CCNGG 3 cut(s) 640, 983, 1892
BmiI GGNNCC 3 cut(s) 395, 474, 979
BmrFI CCNGG 3 cut(s) 640, 983, 1892
BmrI ACTGGG 1 cut(s) 523
BmsI GCATC 1 cut(s) 151
BmuI ACTGGG 1 cut(s) 523
BpiI GAAGAC 5 cut(s) 953, 1130, 1192, 1294, 1958
BplI GAGNNNNNCTC 4 cut(s) 580, 612, 896, 928
BpmI CTGGAG 1 cut(s) 1435
Bpu1102I GCTNAGC 1 cut(s) 794
BpuEI CTTGAG 3 cut(s) 58, 1277, 1381
BpuMI CCSGG 2 cut(s) 640, 983
BsaBI GATNNNNATC 1 cut(s) 498
BsaJI CCNNGG 5 cut(s) 438, 468, 639, 981, 1891
BsaWI WCCGGW 2 cut(s) 316, 1675
BsaXI ACNNNNNCTCC 2 cut(s) 736, 766
Bsc4I CCNNNNNNNGG 2 cut(s) 118, 2063
Bse1I ACTGG 6 cut(s) 518, 623, 1158, 1303, 1331, 1452
Bse3DI GCAATG 2 cut(s) 405, 722
Bse8I GATNNNNATC 1 cut(s) 498
BseAI TCCGGA 1 cut(s) 1675
BseBI CCWGG 1 cut(s) 1892
BseDI CCNNGG 5 cut(s) 438, 468, 639, 981, 1891
BseGI GGATG 2 cut(s) 163, 310
BseJI GATNNNNATC 1 cut(s) 498
BseLI CCNNNNNNNGG 2 cut(s) 118, 2063
BseMI GCAATG 2 cut(s) 405, 722
BseMII CTCAG 3 cut(s) 116, 159, 1007
BseNI ACTGG 6 cut(s) 518, 623, 1158, 1303, 1331, 1452
BseRI GAGGAG 5 cut(s) 845, 1448, 1451, 1628, 1631
BseXI GCAGC 4 cut(s) 294, 1458, 2001, 2040
Bsh1236I CGCG 3 cut(s) 133, 399, 1417
BshFI GGCC 3 cut(s) 836, 1330, 1989
BshNI GGYRCC 2 cut(s) 393, 472
BsiSI CCGG 4 cut(s) 317, 639, 983, 1676
BslFI GGGAC 2 cut(s) 984, 1734
BslI CCNNNNNNNGG 2 cut(s) 118, 2063
BsmAI GTCTC 8 cut(s) 32, 34, 418, 587, 631, 1017, 1081, 1300
BsmFI GGGAC 2 cut(s) 984, 1734
BsmI GAATGC 1 cut(s) 241
BsnI GGCC 3 cut(s) 836, 1330, 1989
Bsp13I TCCGGA 1 cut(s) 1675
Bsp1720I GCTNAGC 1 cut(s) 794
BspACI CCGC 3 cut(s) 397, 1415, 1865
BspANI GGCC 3 cut(s) 836, 1330, 1989
BspCNI CTCAG 3 cut(s) 117, 160, 1008
BspEI TCCGGA 1 cut(s) 1675
BspFNI CGCG 3 cut(s) 133, 399, 1417
BspHI TCATGA 1 cut(s) 76
BspLI GGNNCC 3 cut(s) 395, 474, 979
BspMAI CTGCAG 2 cut(s) 1119, 1249
BspPI GGATC 6 cut(s) 12, 129, 501, 950, 1402, 1973
BspQI GCTCTTC 1 cut(s) 317
BspT107I GGYRCC 2 cut(s) 393, 472
BsrDI GCAATG 2 cut(s) 405, 722
BsrI ACTGG 6 cut(s) 518, 623, 1158, 1303, 1331, 1452
BssECI CCNNGG 5 cut(s) 438, 468, 639, 981, 1891
BssSI CACGAG 2 cut(s) 848, 1845
BssT1I CCWWGG 1 cut(s) 438
Bst2BI CACGAG 2 cut(s) 848, 1845
Bst2UI CCWGG 1 cut(s) 1892
Bst4CI ACNGT 7 cut(s) 429, 488, 918, 1129, 1224, 1586, 2032
Bst6I CTCTTC 7 cut(s) 146, 317, 416, 618, 995, 1419, 2108
BstC8I GCNNGC 4 cut(s) 403, 549, 646, 650
BstDEI CTNAG 7 cut(s) 125, 168, 276, 794, 1016, 1339, 1938
BstENI CCTNNNNNAGG 1 cut(s) 2061
BstF5I GGATG 2 cut(s) 163, 310
BstFNI CGCG 3 cut(s) 133, 399, 1417
BstHHI GCGC 2 cut(s) 133, 401
BstMAI GTCTC 8 cut(s) 32, 34, 418, 587, 631, 1017, 1081, 1300
BstMWI GCNNNNNNNGC 6 cut(s) 402, 407, 1114, 1930, 1939, 2023
BstNI CCWGG 1 cut(s) 1892
BstNSI RCATGY 4 cut(s) 782, 1354, 1377, 1879
BstSCI CCNGG 3 cut(s) 638, 981, 1890
BstSFI CTRYAG 4 cut(s) 1115, 1245, 1644, 1657
BstUI CGCG 3 cut(s) 133, 399, 1417
BstV1I GCAGC 4 cut(s) 294, 1458, 2001, 2040
BstV2I GAAGAC 5 cut(s) 953, 1130, 1192, 1294, 1958
BstX2I RGATCY 3 cut(s) 4, 121, 1407
BstYI RGATCY 3 cut(s) 4, 121, 1407
BsuI GTATCC 1 cut(s) 324
BsuRI GGCC 3 cut(s) 836, 1330, 1989
BtsCI GGATG 2 cut(s) 163, 310
BtsI GCAGTG 1 cut(s) 1504
BtsIMutI CAGTG 3 cut(s) 493, 1338, 1504
Cac8I GCNNGC 4 cut(s) 403, 549, 646, 650
CaiI CAGNNNCTG 1 cut(s) 1643
CciI TCATGA 1 cut(s) 76
CfoI GCGC 2 cut(s) 133, 401
CseI GACGC 2 cut(s) 452, 1406
Csp6I GTAC 5 cut(s) 430, 473, 1266, 1512, 1790
CspCI CAANNNNNGTGG 2 cut(s) 469, 504
CviQI GTAC 5 cut(s) 430, 473, 1266, 1512, 1790
DdeI CTNAG 7 cut(s) 125, 168, 276, 794, 1016, 1339, 1938
Eam1104I CTCTTC 7 cut(s) 146, 317, 416, 618, 995, 1419, 2108
EarI CTCTTC 7 cut(s) 146, 317, 416, 618, 995, 1419, 2108
Eco130I CCWWGG 1 cut(s) 438
Eco57I CTGAAG 3 cut(s) 44, 279, 1545
EcoNI CCTNNNNNAGG 1 cut(s) 2061
EcoRII CCWGG 1 cut(s) 1890
EcoT14I CCWWGG 1 cut(s) 438
EcoT22I ATGCAT 1 cut(s) 1912
ErhI CCWWGG 1 cut(s) 438
FalI AAGNNNNNCTT 6 cut(s) 137, 169, 215, 247, 1177, 1209
FaqI GGGAC 2 cut(s) 984, 1734
FbaI TGATCA 2 cut(s) 1312, 1831
Fnu4HI GCNGC 5 cut(s) 283, 397, 1447, 2015, 2054
FokI GGATG 2 cut(s) 170, 317
Fsp4HI GCNGC 5 cut(s) 283, 397, 1447, 2015, 2054
FspBI CTAG 4 cut(s) 24, 966, 1163, 1862
GlaI GCGC 2 cut(s) 132, 400
GluI GCNGC 5 cut(s) 283, 397, 1447, 2015, 2054
GsuI CTGGAG 1 cut(s) 1435
HaeIII GGCC 3 cut(s) 836, 1330, 1989
HapII CCGG 4 cut(s) 317, 639, 983, 1676
HgaI GACGC 2 cut(s) 452, 1406
HhaI GCGC 2 cut(s) 133, 401
Hin6I GCGC 2 cut(s) 131, 399
HinP1I GCGC 2 cut(s) 131, 399
HincII GTYRAC 2 cut(s) 921, 1063
HindII GTYRAC 2 cut(s) 921, 1063
HindIII AAGCTT 1 cut(s) 791
HpaI GTTAAC 1 cut(s) 1063
HpaII CCGG 4 cut(s) 317, 639, 983, 1676
Hpy166II GTNNAC 4 cut(s) 220, 921, 1063, 2008
Hpy188III TCNNGA 9 cut(s) 77, 256, 497, 1163, 1197, 1294, 1421, 1676, 1690
Hpy8I GTNNAC 4 cut(s) 220, 921, 1063, 2008
HpyAV CCTTC 6 cut(s) 68, 627, 796, 906, 1780, 1993
HpyCH4III ACNGT 7 cut(s) 429, 488, 918, 1129, 1224, 1586, 2032
HpyCH4IV ACGT 2 cut(s) 508, 1236
HpyF10VI GCNNNNNNNGC 6 cut(s) 402, 407, 1114, 1930, 1939, 2023
HpyF3I CTNAG 7 cut(s) 125, 168, 276, 794, 1016, 1339, 1938
HpySE526I ACGT 2 cut(s) 508, 1236
HspAI GCGC 2 cut(s) 131, 399
Kpn2I TCCGGA 1 cut(s) 1675
KpnI GGTACC 1 cut(s) 476
Ksp22I TGATCA 2 cut(s) 1312, 1831
KspAI GTTAAC 1 cut(s) 1063
LguI GCTCTTC 1 cut(s) 317
LmnI GCTCC 2 cut(s) 983, 1454
Lsp1109I GCAGC 4 cut(s) 294, 1458, 2001, 2040
LweI GCATC 1 cut(s) 151
MaeI CTAG 4 cut(s) 24, 966, 1163, 1862
MaeII ACGT 2 cut(s) 508, 1236
MaeIII GTNAC 7 cut(s) 106, 289, 352, 509, 682, 1096, 1652
MflI RGATCY 3 cut(s) 4, 121, 1407
MluCI AATT 5 cut(s) 44, 94, 702, 1204, 1516
MmeI TCCRAC 1 cut(s) 1231
Mph1103I ATGCAT 1 cut(s) 1912
MroI TCCGGA 1 cut(s) 1675
MroXI GAANNNNTTC 4 cut(s) 57, 176, 612, 780
MseI TTAA 7 cut(s) 603, 632, 1041, 1062, 1572, 1589, 1697
MslI CAYNNNNRTG 2 cut(s) 1767, 1773
MspA1I CMGCKG 1 cut(s) 953
MspI CCGG 4 cut(s) 317, 639, 983, 1676
MspR9I CCNGG 3 cut(s) 640, 983, 1892
Mva1269I GAATGC 1 cut(s) 241
MvaI CCWGG 1 cut(s) 1892
MvnI CGCG 3 cut(s) 133, 399, 1417
MwoI GCNNNNNNNGC 6 cut(s) 402, 407, 1114, 1930, 1939, 2023
NciI CCSGG 2 cut(s) 640, 983
NlaIV GGNNCC 3 cut(s) 395, 474, 979
NmeAIII GCCGAG 1 cut(s) 493
NmuCI GTSAC 3 cut(s) 289, 509, 1652
NsiI ATGCAT 1 cut(s) 1912
NspI RCATGY 4 cut(s) 782, 1354, 1377, 1879
PagI TCATGA 1 cut(s) 76
PciI ACATGT 3 cut(s) 778, 1373, 1875
PciSI GCTCTTC 1 cut(s) 317
PcsI WCGNNNNNNNCGW 1 cut(s) 1952
PctI GAATGC 1 cut(s) 241
PdmI GAANNNNTTC 4 cut(s) 57, 176, 612, 780
PfeI GAWTC 5 cut(s) 272, 566, 856, 1529, 1640
PflMI CCANNNNNTGG 1 cut(s) 118
PkrI GCNGC 5 cut(s) 284, 398, 1448, 2016, 2055
PscI ACATGT 3 cut(s) 778, 1373, 1875
PshBI ATTAAT 2 cut(s) 603, 1041
Psp6I CCWGG 1 cut(s) 1890
PspGI CCWGG 1 cut(s) 1890
PspN4I GGNNCC 3 cut(s) 395, 474, 979
PstI CTGCAG 2 cut(s) 1119, 1249
PstNI CAGNNNCTG 1 cut(s) 1643
PsuI RGATCY 3 cut(s) 4, 121, 1407
PvuII CAGCTG 1 cut(s) 953
RsaI GTAC 5 cut(s) 431, 474, 1267, 1513, 1791
RsaNI GTAC 5 cut(s) 430, 473, 1266, 1512, 1790
RseI CAYNNNNRTG 2 cut(s) 1767, 1773
SapI GCTCTTC 1 cut(s) 317
SaqAI TTAA 7 cut(s) 603, 632, 1041, 1062, 1572, 1589, 1697
SatI GCNGC 5 cut(s) 283, 397, 1447, 2015, 2054
ScaI AGTACT 1 cut(s) 431
ScrFI CCNGG 3 cut(s) 640, 983, 1892
SfaNI GCATC 1 cut(s) 151
SfcI CTRYAG 4 cut(s) 1115, 1245, 1644, 1657
SmiMI CAYNNNNRTG 2 cut(s) 1767, 1773
SmlI CTYRAG 3 cut(s) 37, 1292, 1396
SmoI CTYRAG 3 cut(s) 37, 1292, 1396
Sse9I AATT 5 cut(s) 44, 94, 702, 1204, 1516
SsiI CCGC 3 cut(s) 397, 1415, 1865
SspI AATATT 2 cut(s) 558, 1039
SspMI CTAG 4 cut(s) 24, 966, 1163, 1862
StyD4I CCNGG 3 cut(s) 638, 981, 1890
StyI CCWWGG 1 cut(s) 438
TaaI ACNGT 7 cut(s) 429, 488, 918, 1129, 1224, 1586, 2032
TaiI ACGT 2 cut(s) 511, 1239
TaqI TCGA 6 cut(s) 69, 816, 1021, 1196, 1478, 1946
TasI AATT 5 cut(s) 44, 94, 702, 1204, 1516
TatI WGTACW 1 cut(s) 429
TauI GCSGC 1 cut(s) 399
TfiI GAWTC 5 cut(s) 272, 566, 856, 1529, 1640
Tru1I TTAA 7 cut(s) 603, 632, 1041, 1062, 1572, 1589, 1697
Tru9I TTAA 7 cut(s) 603, 632, 1041, 1062, 1572, 1589, 1697
TscAI CASTG 3 cut(s) 493, 1338, 1504
TseFI GTSAC 3 cut(s) 289, 509, 1652
TseI GCWGC 4 cut(s) 282, 1446, 2014, 2053
Tsp45I GTSAC 3 cut(s) 289, 509, 1652
TspGWI ACGGA 1 cut(s) 331
TspRI CASTG 3 cut(s) 493, 1338, 1504
Van91I CCANNNNNTGG 1 cut(s) 118
VspI ATTAAT 2 cut(s) 603, 1041
XagI CCTNNNNNAGG 1 cut(s) 2061
XapI RAATTY 1 cut(s) 94
XbaI TCTAGA 1 cut(s) 1162
XceI RCATGY 4 cut(s) 782, 1354, 1377, 1879
XcmI CCANNNNNNNNNTGG 1 cut(s) 1766
XmnI GAANNNNTTC 4 cut(s) 57, 176, 612, 780
XspI CTAG 4 cut(s) 24, 966, 1163, 1862
ZrmI AGTACT 1 cut(s) 431
Zsp2I ATGCAT 1 cut(s) 1912
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.