Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT5G63920 |
| fragaria_vesca | FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 |
| malus_domestica | MD15G1134700.v1.1 |
| prunus_persica | Prupe.4G275500_v2.0.a1 Prupe.4G275500_v2.0.a1 |
| pyrus_communis | pycom15g12120 pycom15g12150 |
| rosa_chinensis | RchiOBHm_Chr2g0122231 |
| rosa_laevigata | RLG00000018671 |
| rosa_roxburghii | Rroxscaffold_2G00121760 |
| rosa_rugosa | Rorug02G0237200 |
| rosa_samantha | Rh2AG296000 Rh2BG304900 Rh2CG283300 Rh2DG320000 |
| rosa_wichuraiana | Rw1G008520 Rw2G023570 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 653 |
| AccB1I | GGYRCC | 1 cut(s) | 1556 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 205, 2693 |
| AccBSI | CCGCTC | 1 cut(s) | 2583 |
| AccI | GTMKAC | 2 cut(s) | 2094, 2587 |
| AccII | CGCG | 2 cut(s) | 7, 2649 |
| AccIII | TCCGGA | 1 cut(s) | 99 |
| AclWI | GGATC | 7 cut(s) | 503, 706, 1117, 2211, 2224, 2437, 2714 |
| AcoI | YGGCCR | 3 cut(s) | 196, 2608, 2626 |
| AcsI | RAATTY | 2 cut(s) | 2127, 2365 |
| AcuI | CTGAAG | 2 cut(s) | 804, 1788 |
| AdeI | CACNNNGTG | 1 cut(s) | 2438 |
| AfaI | GTAC | 7 cut(s) | 125, 266, 1033, 1400, 2248, 2407, 2449 |
| AfeI | AGCGCT | 1 cut(s) | 1349 |
| AflIII | ACRYGT | 2 cut(s) | 286, 1781 |
| AjiI | CACGTC | 1 cut(s) | 2591 |
| AjnI | CCWGG | 6 cut(s) | 714, 1647, 2029, 2083, 2134, 2242 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 1198, 1230, 1731, 1763 |
| AleI | CACNNNNGTG | 1 cut(s) | 1510 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 200, 232 |
| Alw21I | GWGCWC | 1 cut(s) | 978 |
| Alw26I | GTCTC | 3 cut(s) | 123, 1031, 2674 |
| AlwI | GGATC | 7 cut(s) | 503, 706, 1117, 2211, 2224, 2437, 2714 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 1310, 2249 |
| Ama87I | CYCGRG | 2 cut(s) | 105, 293 |
| Aor13HI | TCCGGA | 1 cut(s) | 99 |
| Aor51HI | AGCGCT | 1 cut(s) | 1349 |
| AoxI | GGCC | 8 cut(s) | 19, 196, 772, 1144, 1496, 2086, 2608, 2626 |
| ApeKI | GCWGC | 8 cut(s) | 163, 598, 1310, 1450, 1484, 1789, 2505, 2595 |
| ApoI | RAATTY | 2 cut(s) | 2127, 2365 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 2356, 2388 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 492 |
| AspLEI | GCGC | 2 cut(s) | 1350, 1660 |
| AspS9I | GGNCC | 6 cut(s) | 20, 662, 1144, 2033, 2081, 2087 |
| AsuC2I | CCSGG | 3 cut(s) | 254, 1967, 2090 |
| AsuHPI | GGTGA | 9 cut(s) | 395, 644, 749, 1181, 1193, 1238, 1355, 1664, 1919 |
| AvaI | CYCGRG | 2 cut(s) | 105, 293 |
| AvaII | GGWCC | 3 cut(s) | 662, 2033, 2081 |
| AxyI | CCTNAGG | 1 cut(s) | 1137 |
| BamHI | GGATCC | 1 cut(s) | 2216 |
| BanI | GGYRCC | 1 cut(s) | 1556 |
| BanII | GRGCYC | 2 cut(s) | 454, 1717 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1509, 1541 |
| BbsI | GAAGAC | 3 cut(s) | 323, 1763, 2482 |
| Bbv12I | GWGCWC | 1 cut(s) | 978 |
| BbvCI | CCTCAGC | 1 cut(s) | 528 |
| BbvI | GCAGC | 8 cut(s) | 175, 585, 1297, 1462, 1496, 1801, 2492, 2607 |
| BccI | CCATC | 4 cut(s) | 161, 199, 1660, 1964 |
| BceAI | ACGGC | 4 cut(s) | 37, 151, 2583, 2595 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 152, 186 |
| BciT130I | CCWGG | 6 cut(s) | 716, 1649, 2031, 2085, 2136, 2244 |
| BciVI | GTATCC | 1 cut(s) | 915 |
| BcnI | CCSGG | 3 cut(s) | 254, 1967, 2090 |
| BcoDI | GTCTC | 3 cut(s) | 123, 1031, 2674 |
| BfaI | CTAG | 6 cut(s) | 543, 582, 1218, 1265, 1283, 1841 |
| BfmI | CTRYAG | 3 cut(s) | 534, 1320, 2506 |
| BfoI | RGCGCY | 2 cut(s) | 1351, 1661 |
| BfuI | GTATCC | 1 cut(s) | 915 |
| BglII | AGATCT | 2 cut(s) | 259, 1868 |
| Bme1390I | CCNGG | 9 cut(s) | 254, 716, 1649, 1967, 2031, 2085, 2090, 2136, 2244 |
| Bme18I | GGWCC | 3 cut(s) | 662, 2033, 2081 |
| BmeT110I | CYCGRG | 2 cut(s) | 105, 293 |
| BmgBI | CACGTC | 1 cut(s) | 2591 |
| BmgT120I | GGNCC | 6 cut(s) | 20, 662, 1144, 2033, 2081, 2087 |
| BmiI | GGNNCC | 6 cut(s) | 22, 855, 1145, 1558, 2218, 2352 |
| BmrFI | CCNGG | 9 cut(s) | 254, 716, 1649, 1967, 2031, 2085, 2090, 2136, 2244 |
| BmrI | ACTGGG | 1 cut(s) | 715 |
| BmuI | ACTGGG | 1 cut(s) | 715 |
| BoxI | GACNNNNGTC | 1 cut(s) | 188 |
| BpiI | GAAGAC | 3 cut(s) | 323, 1763, 2482 |
| BpmI | CTGGAG | 4 cut(s) | 1171, 1255, 2226, 2700 |
| Bpu10I | CCTNAGC | 1 cut(s) | 528 |
| BpuEI | CTTGAG | 3 cut(s) | 233, 1412, 2211 |
| BpuMI | CCSGG | 3 cut(s) | 254, 1967, 2090 |
| BsaAI | YACGTR | 1 cut(s) | 1070 |
| BsaBI | GATNNNNATC | 4 cut(s) | 513, 1334, 2148, 2201 |
| BsaI | GGTCTC | 2 cut(s) | 123, 1031 |
| BsaJI | CCNNGG | 8 cut(s) | 57, 88, 294, 887, 1648, 2269, 2599, 2611 |
| BsaWI | WCCGGW | 2 cut(s) | 99, 274 |
| BsaXI | ACNNNNNCTCC | 8 cut(s) | 38, 68, 101, 131, 200, 230, 2673, 2703 |
| Bse1I | ACTGG | 6 cut(s) | 353, 710, 1238, 2218, 2683, 2687 |
| Bse21I | CCTNAGG | 1 cut(s) | 1137 |
| Bse3DI | GCAATG | 3 cut(s) | 390, 1854, 1942 |
| Bse8I | GATNNNNATC | 4 cut(s) | 513, 1334, 2148, 2201 |
| BseAI | TCCGGA | 1 cut(s) | 99 |
| BseBI | CCWGG | 6 cut(s) | 716, 1649, 2031, 2085, 2136, 2244 |
| BseDI | CCNNGG | 8 cut(s) | 57, 88, 294, 887, 1648, 2269, 2599, 2611 |
| BseGI | GGATG | 5 cut(s) | 798, 883, 1096, 1624, 1975 |
| BseJI | GATNNNNATC | 4 cut(s) | 513, 1334, 2148, 2201 |
| BseMI | GCAATG | 3 cut(s) | 390, 1854, 1942 |
| BseMII | CTCAG | 8 cut(s) | 519, 593, 956, 1128, 1520, 1723, 1969, 2018 |
| BseNI | ACTGG | 6 cut(s) | 353, 710, 1238, 2218, 2683, 2687 |
| BseRI | GAGGAG | 4 cut(s) | 341, 344, 650, 655 |
| BseXI | GCAGC | 8 cut(s) | 175, 585, 1297, 1462, 1496, 1801, 2492, 2607 |
| BseYI | CCCAGC | 1 cut(s) | 1296 |
| BsgI | GTGCAG | 1 cut(s) | 2235 |
| Bsh1236I | CGCG | 2 cut(s) | 7, 2649 |
| Bsh1285I | CGRYCG | 1 cut(s) | 226 |
| BshFI | GGCC | 8 cut(s) | 21, 198, 774, 1146, 1498, 2088, 2610, 2628 |
| BshNI | GGYRCC | 1 cut(s) | 1556 |
| BsiEI | CGRYCG | 1 cut(s) | 226 |
| BsiHKAI | GWGCWC | 1 cut(s) | 978 |
| BsiHKCI | CYCGRG | 2 cut(s) | 105, 293 |
| BsiSI | CCGG | 5 cut(s) | 100, 254, 275, 1966, 2090 |
| BsiWI | CGTACG | 1 cut(s) | 1031 |
| BslFI | GGGAC | 2 cut(s) | 863, 2325 |
| BsmAI | GTCTC | 3 cut(s) | 123, 1031, 2674 |
| BsmFI | GGGAC | 2 cut(s) | 863, 2325 |
| BsmI | GAATGC | 2 cut(s) | 2020, 2557 |
| BsnI | GGCC | 8 cut(s) | 21, 198, 774, 1146, 1498, 2088, 2610, 2628 |
| Bso31I | GGTCTC | 2 cut(s) | 123, 1031 |
| BsoBI | CYCGRG | 2 cut(s) | 105, 293 |
| Bsp1286I | GDGCHC | 3 cut(s) | 454, 978, 1717 |
| Bsp13I | TCCGGA | 1 cut(s) | 99 |
| Bsp68I | TCGCGA | 1 cut(s) | 7 |
| BspANI | GGCC | 8 cut(s) | 21, 198, 774, 1146, 1498, 2088, 2610, 2628 |
| BspCNI | CTCAG | 8 cut(s) | 520, 594, 957, 1129, 1521, 1722, 1968, 2017 |
| BspEI | TCCGGA | 1 cut(s) | 99 |
| BspFNI | CGCG | 2 cut(s) | 7, 2649 |
| BspHI | TCATGA | 3 cut(s) | 279, 484, 1174 |
| BspLI | GGNNCC | 6 cut(s) | 22, 855, 1145, 1558, 2218, 2352 |
| BspMAI | CTGCAG | 1 cut(s) | 2510 |
| BspPI | GGATC | 7 cut(s) | 503, 706, 1117, 2211, 2224, 2437, 2714 |
| BspT107I | GGYRCC | 1 cut(s) | 1556 |
| BspTNI | GGTCTC | 2 cut(s) | 123, 1031 |
| BsrBI | CCGCTC | 1 cut(s) | 2583 |
| BsrDI | GCAATG | 3 cut(s) | 390, 1854, 1942 |
| BsrI | ACTGG | 6 cut(s) | 353, 710, 1238, 2218, 2683, 2687 |
| BssECI | CCNNGG | 8 cut(s) | 57, 88, 294, 887, 1648, 2269, 2599, 2611 |
| BssT1I | CCWWGG | 2 cut(s) | 887, 2269 |
| Bst2UI | CCWGG | 6 cut(s) | 716, 1649, 2031, 2085, 2136, 2244 |
| Bst6I | CTCTTC | 2 cut(s) | 726, 812 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 1310, 1932 |
| BstBAI | YACGTR | 1 cut(s) | 1070 |
| BstC8I | GCNNGC | 2 cut(s) | 1302, 1549 |
| BstDSI | CCRYGG | 4 cut(s) | 57, 88, 2599, 2611 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1652 |
| BstF5I | GGATG | 5 cut(s) | 798, 883, 1096, 1624, 1975 |
| BstFNI | CGCG | 2 cut(s) | 7, 2649 |
| BstH2I | RGCGCY | 2 cut(s) | 1351, 1661 |
| BstHHI | GCGC | 2 cut(s) | 1350, 1660 |
| BstMAI | GTCTC | 3 cut(s) | 123, 1031, 2674 |
| BstMCI | CGRYCG | 1 cut(s) | 226 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 539, 1310, 1376, 1932, 2169, 2580, 2631 |
| BstNI | CCWGG | 6 cut(s) | 716, 1649, 2031, 2085, 2136, 2244 |
| BstNSI | RCATGY | 3 cut(s) | 1290, 1785, 2327 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 188 |
| BstSCI | CCNGG | 9 cut(s) | 252, 714, 1647, 1965, 2029, 2083, 2088, 2134, 2242 |
| BstSFI | CTRYAG | 3 cut(s) | 534, 1320, 2506 |
| BstUI | CGCG | 2 cut(s) | 7, 2649 |
| BstV1I | GCAGC | 8 cut(s) | 175, 585, 1297, 1462, 1496, 1801, 2492, 2607 |
| BstV2I | GAAGAC | 3 cut(s) | 323, 1763, 2482 |
| BstX2I | RGATCY | 5 cut(s) | 259, 508, 711, 1868, 2216 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 988, 2636 |
| BstYI | RGATCY | 5 cut(s) | 259, 508, 711, 1868, 2216 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1137 |
| BsuI | GTATCC | 1 cut(s) | 915 |
| BsuRI | GGCC | 8 cut(s) | 21, 198, 774, 1146, 1498, 2088, 2610, 2628 |
| BtgI | CCRYGG | 4 cut(s) | 57, 88, 2599, 2611 |
| BtrI | CACGTC | 1 cut(s) | 2591 |
| BtsCI | GGATG | 5 cut(s) | 798, 883, 1096, 1624, 1975 |
| BtsI | GCAGTG | 1 cut(s) | 2641 |
| BtsIMutI | CAGTG | 8 cut(s) | 36, 75, 864, 1431, 1878, 2012, 2641, 2694 |
| BtuMI | TCGCGA | 1 cut(s) | 7 |
| Cac8I | GCNNGC | 2 cut(s) | 1302, 1549 |
| CaiI | CAGNNNCTG | 2 cut(s) | 1310, 2249 |
| CciI | TCATGA | 3 cut(s) | 279, 484, 1174 |
| CfoI | GCGC | 2 cut(s) | 1350, 1660 |
| Cfr13I | GGNCC | 6 cut(s) | 20, 662, 1144, 2033, 2081, 2087 |
| CseI | GACGC | 1 cut(s) | 2655 |
| Csp6I | GTAC | 7 cut(s) | 124, 265, 1032, 1399, 2247, 2406, 2448 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 1491, 1526, 2527, 2562 |
| CviQI | GTAC | 7 cut(s) | 124, 265, 1032, 1399, 2247, 2406, 2448 |
| DraIII | CACNNNGTG | 1 cut(s) | 2438 |
| EaeI | YGGCCR | 3 cut(s) | 196, 2608, 2626 |
| Eam1104I | CTCTTC | 2 cut(s) | 726, 812 |
| EarI | CTCTTC | 2 cut(s) | 726, 812 |
| EciI | GGCGGA | 2 cut(s) | 59, 1262 |
| Eco130I | CCWWGG | 2 cut(s) | 887, 2269 |
| Eco147I | AGGCCT | 1 cut(s) | 774 |
| Eco24I | GRGCYC | 2 cut(s) | 454, 1717 |
| Eco31I | GGTCTC | 2 cut(s) | 123, 1031 |
| Eco32I | GATATC | 1 cut(s) | 1336 |
| Eco47I | GGWCC | 3 cut(s) | 662, 2033, 2081 |
| Eco47III | AGCGCT | 1 cut(s) | 1349 |
| Eco57I | CTGAAG | 2 cut(s) | 804, 1788 |
| Eco81I | CCTNAGG | 1 cut(s) | 1137 |
| Eco88I | CYCGRG | 2 cut(s) | 105, 293 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1652 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1144 |
| EcoRI | GAATTC | 1 cut(s) | 2127 |
| EcoRII | CCWGG | 6 cut(s) | 714, 1647, 2029, 2083, 2134, 2242 |
| EcoRV | GATATC | 1 cut(s) | 1336 |
| EcoT14I | CCWWGG | 2 cut(s) | 887, 2269 |
| EcoT38I | GRGCYC | 2 cut(s) | 454, 1717 |
| ErhI | CCWWGG | 2 cut(s) | 887, 2269 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 759, 791 |
| FaqI | GGGAC | 2 cut(s) | 863, 2325 |
| FauI | CCCGC | 2 cut(s) | 67, 2570 |
| FauNDI | CATATG | 1 cut(s) | 1114 |
| FblI | GTMKAC | 2 cut(s) | 2094, 2587 |
| FokI | GGATG | 5 cut(s) | 805, 870, 1083, 1631, 1982 |
| FriOI | GRGCYC | 2 cut(s) | 454, 1717 |
| FspBI | CTAG | 6 cut(s) | 543, 582, 1218, 1265, 1283, 1841 |
| GlaI | GCGC | 2 cut(s) | 1349, 1659 |
| GsaI | CCCAGC | 1 cut(s) | 1300 |
| GsuI | CTGGAG | 4 cut(s) | 1171, 1255, 2226, 2700 |
| HaeII | RGCGCY | 2 cut(s) | 1351, 1661 |
| HaeIII | GGCC | 8 cut(s) | 21, 198, 774, 1146, 1498, 2088, 2610, 2628 |
| HapII | CCGG | 5 cut(s) | 100, 254, 275, 1966, 2090 |
| HgaI | GACGC | 1 cut(s) | 2655 |
| HhaI | GCGC | 2 cut(s) | 1350, 1660 |
| Hin6I | GCGC | 2 cut(s) | 1348, 1658 |
| HinP1I | GCGC | 2 cut(s) | 1348, 1658 |
| HindIII | AAGCTT | 2 cut(s) | 1800, 2662 |
| HpaII | CCGG | 5 cut(s) | 100, 254, 275, 1966, 2090 |
| HphI | GGTGA | 9 cut(s) | 395, 644, 749, 1181, 1193, 1238, 1355, 1664, 1919 |
| Hpy166II | GTNNAC | 3 cut(s) | 1399, 2095, 2588 |
| Hpy8I | GTNNAC | 3 cut(s) | 1399, 2095, 2588 |
| Hpy99I | CGWCG | 3 cut(s) | 12, 1923, 2649 |
| HpyAV | CCTTC | 4 cut(s) | 374, 764, 979, 2733 |
| HpyCH4IV | ACGT | 5 cut(s) | 39, 288, 943, 1069, 2590 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 539, 1310, 1376, 1932, 2169, 2580, 2631 |
| HpySE526I | ACGT | 5 cut(s) | 39, 288, 943, 1069, 2590 |
| HspAI | GCGC | 2 cut(s) | 1348, 1658 |
| Kpn2I | TCCGGA | 1 cut(s) | 99 |
| LmnI | GCTCC | 5 cut(s) | 208, 277, 1236, 1842, 2580 |
| Lsp1109I | GCAGC | 8 cut(s) | 175, 585, 1297, 1462, 1496, 1801, 2492, 2607 |
| MaeI | CTAG | 6 cut(s) | 543, 582, 1218, 1265, 1283, 1841 |
| MaeII | ACGT | 5 cut(s) | 39, 288, 943, 1069, 2590 |
| MaeIII | GTNAC | 6 cut(s) | 25, 227, 1489, 2053, 2417, 2768 |
| MbiI | CCGCTC | 1 cut(s) | 2583 |
| MfeI | CAATTG | 1 cut(s) | 518 |
| MflI | RGATCY | 5 cut(s) | 259, 508, 711, 1868, 2216 |
| MhlI | GDGCHC | 3 cut(s) | 454, 978, 1717 |
| MlyI | GAGTC | 5 cut(s) | 97, 617, 652, 1235, 1468 |
| MmeI | TCCRAC | 4 cut(s) | 1664, 1942, 1991, 2519 |
| MroI | TCCGGA | 1 cut(s) | 99 |
| MroXI | GAANNNNTTC | 1 cut(s) | 492 |
| MseI | TTAA | 6 cut(s) | 312, 467, 786, 1251, 2148, 2187 |
| MslI | CAYNNNNRTG | 4 cut(s) | 869, 986, 1251, 1510 |
| MspA1I | CMGCKG | 3 cut(s) | 824, 1487, 1904 |
| MspI | CCGG | 5 cut(s) | 100, 254, 275, 1966, 2090 |
| MspR9I | CCNGG | 9 cut(s) | 254, 716, 1649, 1967, 2031, 2085, 2090, 2136, 2244 |
| MunI | CAATTG | 1 cut(s) | 518 |
| Mva1269I | GAATGC | 2 cut(s) | 2020, 2557 |
| MvaI | CCWGG | 6 cut(s) | 716, 1649, 2031, 2085, 2136, 2244 |
| MvnI | CGCG | 2 cut(s) | 7, 2649 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 539, 1310, 1376, 1932, 2169, 2580, 2631 |
| NciI | CCSGG | 3 cut(s) | 254, 1967, 2090 |
| NdeI | CATATG | 1 cut(s) | 1114 |
| NlaIV | GGNNCC | 6 cut(s) | 22, 855, 1145, 1558, 2218, 2352 |
| NmuCI | GTSAC | 2 cut(s) | 25, 2053 |
| NruI | TCGCGA | 1 cut(s) | 7 |
| NspI | RCATGY | 3 cut(s) | 1290, 1785, 2327 |
| OliI | CACNNNNGTG | 1 cut(s) | 1510 |
| PagI | TCATGA | 3 cut(s) | 279, 484, 1174 |
| PceI | AGGCCT | 1 cut(s) | 774 |
| PciI | ACATGT | 1 cut(s) | 1781 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 2368, 2596 |
| PctI | GAATGC | 2 cut(s) | 2020, 2557 |
| PdmI | GAANNNNTTC | 1 cut(s) | 492 |
| PfeI | GAWTC | 7 cut(s) | 79, 499, 897, 1043, 1409, 1429, 1940 |
| Pfl23II | CGTACG | 1 cut(s) | 1031 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 205, 2693 |
| PfoI | TCCNGGA | 1 cut(s) | 2029 |
| Ple19I | CGATCG | 1 cut(s) | 226 |
| PleI | GAGTC | 5 cut(s) | 97, 617, 651, 1234, 1468 |
| PpsI | GAGTC | 5 cut(s) | 97, 617, 651, 1234, 1468 |
| Ppu21I | YACGTR | 1 cut(s) | 1070 |
| PscI | ACATGT | 1 cut(s) | 1781 |
| PshAI | GACNNNNGTC | 1 cut(s) | 188 |
| PsiI | TTATAA | 1 cut(s) | 653 |
| Psp6I | CCWGG | 6 cut(s) | 714, 1647, 2029, 2083, 2134, 2242 |
| PspFI | CCCAGC | 1 cut(s) | 1296 |
| PspGI | CCWGG | 6 cut(s) | 714, 1647, 2029, 2083, 2134, 2242 |
| PspLI | CGTACG | 1 cut(s) | 1031 |
| PspN4I | GGNNCC | 6 cut(s) | 22, 855, 1145, 1558, 2218, 2352 |
| PspPI | GGNCC | 6 cut(s) | 20, 662, 1144, 2033, 2081, 2087 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 2230, 2262 |
| PstI | CTGCAG | 1 cut(s) | 2510 |
| PstNI | CAGNNNCTG | 2 cut(s) | 1310, 2249 |
| PsuI | RGATCY | 5 cut(s) | 259, 508, 711, 1868, 2216 |
| PvuI | CGATCG | 1 cut(s) | 226 |
| PvuII | CAGCTG | 3 cut(s) | 824, 1487, 1904 |
| RruI | TCGCGA | 1 cut(s) | 7 |
| RsaI | GTAC | 7 cut(s) | 125, 266, 1033, 1400, 2248, 2407, 2449 |
| RsaNI | GTAC | 7 cut(s) | 124, 265, 1032, 1399, 2247, 2406, 2448 |
| RseI | CAYNNNNRTG | 4 cut(s) | 869, 986, 1251, 1510 |
| SaqAI | TTAA | 6 cut(s) | 312, 467, 786, 1251, 2148, 2187 |
| Sau96I | GGNCC | 6 cut(s) | 20, 662, 1144, 2033, 2081, 2087 |
| SchI | GAGTC | 5 cut(s) | 97, 617, 652, 1235, 1468 |
| ScrFI | CCNGG | 9 cut(s) | 254, 716, 1649, 1967, 2031, 2085, 2090, 2136, 2244 |
| SduI | GDGCHC | 3 cut(s) | 454, 978, 1717 |
| SfcI | CTRYAG | 3 cut(s) | 534, 1320, 2506 |
| SinI | GGWCC | 3 cut(s) | 662, 2033, 2081 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 869, 986, 1251, 1510 |
| SmlI | CTYRAG | 3 cut(s) | 212, 1391, 2226 |
| SmoI | CTYRAG | 3 cut(s) | 212, 1391, 2226 |
| SseBI | AGGCCT | 1 cut(s) | 774 |
| SspI | AATATT | 1 cut(s) | 1374 |
| SspMI | CTAG | 6 cut(s) | 543, 582, 1218, 1265, 1283, 1841 |
| StuI | AGGCCT | 1 cut(s) | 774 |
| StyD4I | CCNGG | 9 cut(s) | 252, 714, 1647, 1965, 2029, 2083, 2088, 2134, 2242 |
| StyI | CCWWGG | 2 cut(s) | 887, 2269 |
| TaiI | ACGT | 5 cut(s) | 42, 291, 946, 1072, 2593 |
| TaqI | TCGA | 8 cut(s) | 138, 144, 696, 810, 1052, 1326, 1479, 2362 |
| TaqII | GACCGA | 2 cut(s) | 1055, 2050 |
| TatI | WGTACW | 1 cut(s) | 2405 |
| TauI | GCSGC | 3 cut(s) | 2577, 2628, 2652 |
| TfiI | GAWTC | 7 cut(s) | 79, 499, 897, 1043, 1409, 1429, 1940 |
| Tru1I | TTAA | 6 cut(s) | 312, 467, 786, 1251, 2148, 2187 |
| Tru9I | TTAA | 6 cut(s) | 312, 467, 786, 1251, 2148, 2187 |
| TscAI | CASTG | 8 cut(s) | 36, 75, 871, 1431, 1885, 2012, 2641, 2694 |
| TseFI | GTSAC | 2 cut(s) | 25, 2053 |
| TseI | GCWGC | 8 cut(s) | 163, 598, 1310, 1450, 1484, 1789, 2505, 2595 |
| Tsp45I | GTSAC | 2 cut(s) | 25, 2053 |
| TspGWI | ACGGA | 6 cut(s) | 46, 178, 654, 950, 1049, 2105 |
| TspRI | CASTG | 8 cut(s) | 36, 75, 871, 1431, 1885, 2012, 2641, 2694 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 205, 2693 |
| VpaK11BI | GGWCC | 3 cut(s) | 662, 2033, 2081 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1652 |
| XapI | RAATTY | 2 cut(s) | 2127, 2365 |
| XceI | RCATGY | 3 cut(s) | 1290, 1785, 2327 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 735 |
| XmiI | GTMKAC | 2 cut(s) | 2094, 2587 |
| XmnI | GAANNNNTTC | 1 cut(s) | 492 |
| XspI | CTAG | 6 cut(s) | 543, 582, 1218, 1265, 1283, 1841 |