Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT5G63920 |
| fragaria_vesca | FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 |
| malus_domestica | MD15G1134700.v1.1 |
| prunus_persica | Prupe.4G275500_v2.0.a1 Prupe.4G275500_v2.0.a1 |
| pyrus_communis | pycom15g12120 pycom15g12150 |
| rosa_chinensis | RchiOBHm_Chr2g0122231 |
| rosa_laevigata | RLG00000018671 |
| rosa_roxburghii | Rroxscaffold_2G00121760 |
| rosa_rugosa | Rorug02G0237200 |
| rosa_samantha | Rh2AG296000 Rh2BG304900 Rh2CG283300 Rh2DG320000 |
| rosa_wichuraiana | Rw1G008520 Rw2G023570 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 70 |
| AccB1I | GGYRCC | 1 cut(s) | 339 |
| AccB7I | CCANNNNNTGG | 3 cut(s) | 202, 1136, 1276 |
| AccBSI | CCGCTC | 1 cut(s) | 269 |
| AclI | AACGTT | 1 cut(s) | 2058 |
| AclWI | GGATC | 6 cut(s) | 108, 1111, 1599, 2035, 2825, 2861 |
| AcoI | YGGCCR | 3 cut(s) | 157, 193, 2779 |
| AcsI | RAATTY | 5 cut(s) | 212, 515, 1205, 1969, 2633 |
| AcuI | CTGAAG | 6 cut(s) | 1338, 1782, 2304, 2328, 2466, 2490 |
| AdeI | CACNNNGTG | 1 cut(s) | 2591 |
| AfaI | GTAC | 4 cut(s) | 998, 1027, 1947, 2544 |
| AflIII | ACRYGT | 1 cut(s) | 2576 |
| AgsI | TTSAA | 8 cut(s) | 142, 398, 932, 1403, 1819, 1838, 2099, 2384 |
| AjnI | CCWGG | 5 cut(s) | 1093, 1275, 2023, 2077, 2276 |
| AleI | CACNNNNGTG | 1 cut(s) | 419 |
| Alw21I | GWGCWC | 2 cut(s) | 210, 2587 |
| Alw26I | GTCTC | 3 cut(s) | 1291, 2168, 2842 |
| Alw44I | GTGCAC | 1 cut(s) | 2583 |
| AlwI | GGATC | 6 cut(s) | 108, 1111, 1599, 2035, 2825, 2861 |
| AlwNI | CAGNNNCTG | 4 cut(s) | 1136, 2042, 2084, 2825 |
| ApaLI | GTGCAC | 1 cut(s) | 2583 |
| ApeKI | GCWGC | 6 cut(s) | 595, 1585, 1783, 2042, 2766, 2798 |
| ApoI | RAATTY | 5 cut(s) | 212, 515, 1205, 1969, 2633 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 2209 |
| AsuC2I | CCSGG | 2 cut(s) | 1961, 2783 |
| AsuHPI | GGTGA | 9 cut(s) | 172, 181, 1232, 1349, 1623, 1669, 2371, 2818, 2840 |
| AsuII | TTCGAA | 1 cut(s) | 229 |
| AvaII | GGWCC | 9 cut(s) | 220, 659, 738, 1126, 2081, 2285, 2348, 2402, 2510 |
| AxyI | CCTNAGG | 1 cut(s) | 291 |
| BaeGI | GKGCMC | 1 cut(s) | 2587 |
| BanI | GGYRCC | 1 cut(s) | 339 |
| BanII | GRGCYC | 2 cut(s) | 210, 451 |
| BauI | CACGAG | 1 cut(s) | 719 |
| Bbv12I | GWGCWC | 2 cut(s) | 210, 2587 |
| BbvCI | CCTCAGC | 1 cut(s) | 2737 |
| BbvI | GCAGC | 6 cut(s) | 582, 1572, 1795, 2029, 2778, 2785 |
| BccI | CCATC | 8 cut(s) | 49, 1031, 1092, 1191, 1221, 1654, 1958, 2878 |
| BceAI | ACGGC | 1 cut(s) | 404 |
| BciT130I | CCWGG | 5 cut(s) | 1095, 1277, 2025, 2079, 2278 |
| BclI | TGATCA | 2 cut(s) | 1570, 1885 |
| BcnI | CCSGG | 2 cut(s) | 1961, 2783 |
| BcoDI | GTCTC | 3 cut(s) | 1291, 2168, 2842 |
| BfaI | CTAG | 9 cut(s) | 540, 579, 1155, 1370, 1488, 1805, 1875, 2117, 2439 |
| BfmI | CTRYAG | 2 cut(s) | 1299, 2134 |
| BglII | AGATCT | 2 cut(s) | 553, 1862 |
| BisI | GCNGC | 9 cut(s) | 160, 596, 1586, 1784, 2043, 2114, 2164, 2767, 2799 |
| BlsI | GCNGC | 9 cut(s) | 161, 597, 1587, 1785, 2044, 2115, 2165, 2768, 2800 |
| Bme1390I | CCNGG | 7 cut(s) | 1095, 1277, 1961, 2025, 2079, 2278, 2783 |
| Bme18I | GGWCC | 9 cut(s) | 220, 659, 738, 1126, 2081, 2285, 2348, 2402, 2510 |
| BmiI | GGNNCC | 6 cut(s) | 80, 341, 852, 1128, 2021, 2286 |
| BmrFI | CCNGG | 7 cut(s) | 1095, 1277, 1961, 2025, 2079, 2278, 2783 |
| BpmI | CTGGAG | 2 cut(s) | 1236, 1920 |
| Bpu10I | CCTNAGC | 1 cut(s) | 2737 |
| Bpu14I | TTCGAA | 1 cut(s) | 229 |
| BpuEI | CTTGAG | 1 cut(s) | 1600 |
| BpuMI | CCSGG | 2 cut(s) | 1961, 2783 |
| Bsa29I | ATCGAT | 2 cut(s) | 1396, 1594 |
| BsaAI | YACGTR | 1 cut(s) | 2577 |
| BsaBI | GATNNNNATC | 3 cut(s) | 1102, 1402, 2032 |
| BsaJI | CCNNGG | 5 cut(s) | 82, 1608, 2023, 2782, 2899 |
| BsaWI | WCCGGW | 1 cut(s) | 2840 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 436, 466, 1477, 1507 |
| Bse118I | RCCGGY | 1 cut(s) | 191 |
| Bse1I | ACTGG | 4 cut(s) | 343, 667, 2212, 2830 |
| Bse21I | CCTNAGG | 1 cut(s) | 291 |
| Bse3DI | GCAATG | 4 cut(s) | 387, 771, 1165, 2415 |
| Bse8I | GATNNNNATC | 3 cut(s) | 1102, 1402, 2032 |
| BseBI | CCWGG | 5 cut(s) | 1095, 1277, 2025, 2079, 2278 |
| BseCI | ATCGAT | 2 cut(s) | 1396, 1594 |
| BseDI | CCNNGG | 5 cut(s) | 82, 1608, 2023, 2782, 2899 |
| BseJI | GATNNNNATC | 3 cut(s) | 1102, 1402, 2032 |
| BseMI | GCAATG | 4 cut(s) | 387, 771, 1165, 2415 |
| BseMII | CTCAG | 7 cut(s) | 86, 282, 471, 590, 1514, 2012, 2751 |
| BseNI | ACTGG | 4 cut(s) | 343, 667, 2212, 2830 |
| BseRI | GAGGAG | 2 cut(s) | 338, 743 |
| BseSI | GKGCMC | 1 cut(s) | 2587 |
| BseXI | GCAGC | 6 cut(s) | 582, 1572, 1795, 2029, 2778, 2785 |
| BseYI | CCCAGC | 2 cut(s) | 1129, 2801 |
| BsgI | GTGCAG | 2 cut(s) | 512, 2808 |
| BshNI | GGYRCC | 1 cut(s) | 339 |
| BshVI | ATCGAT | 2 cut(s) | 1396, 1594 |
| BsiHKAI | GWGCWC | 2 cut(s) | 210, 2587 |
| BsiSI | CCGG | 4 cut(s) | 192, 1960, 2782, 2841 |
| BsiWI | CGTACG | 1 cut(s) | 1025 |
| BslFI | GGGAC | 3 cut(s) | 272, 1439, 2246 |
| BsmAI | GTCTC | 3 cut(s) | 1291, 2168, 2842 |
| BsmBI | CGTCTC | 1 cut(s) | 2842 |
| BsmFI | GGGAC | 3 cut(s) | 272, 1439, 2246 |
| BsmI | GAATGC | 2 cut(s) | 2014, 2314 |
| Bsp119I | TTCGAA | 1 cut(s) | 229 |
| Bsp1286I | GDGCHC | 3 cut(s) | 210, 451, 2587 |
| Bsp1407I | TGTACA | 1 cut(s) | 2542 |
| BspCNI | CTCAG | 7 cut(s) | 87, 283, 470, 591, 1515, 2011, 2750 |
| BspDI | ATCGAT | 2 cut(s) | 1396, 1594 |
| BspLI | GGNNCC | 6 cut(s) | 80, 341, 852, 1128, 2021, 2286 |
| BspPI | GGATC | 6 cut(s) | 108, 1111, 1599, 2035, 2825, 2861 |
| BspQI | GCTCTTC | 1 cut(s) | 984 |
| BspT104I | TTCGAA | 1 cut(s) | 229 |
| BspT107I | GGYRCC | 1 cut(s) | 339 |
| BsrBI | CCGCTC | 1 cut(s) | 269 |
| BsrDI | GCAATG | 4 cut(s) | 387, 771, 1165, 2415 |
| BsrFI | RCCGGY | 1 cut(s) | 191 |
| BsrGI | TGTACA | 1 cut(s) | 2542 |
| BsrI | ACTGG | 4 cut(s) | 343, 667, 2212, 2830 |
| BssAI | RCCGGY | 1 cut(s) | 191 |
| BssECI | CCNNGG | 5 cut(s) | 82, 1608, 2023, 2782, 2899 |
| BssSI | CACGAG | 1 cut(s) | 719 |
| BssT1I | CCWWGG | 2 cut(s) | 82, 1608 |
| Bst2BI | CACGAG | 1 cut(s) | 719 |
| Bst2UI | CCWGG | 5 cut(s) | 1095, 1277, 2025, 2079, 2278 |
| Bst6I | CTCTTC | 2 cut(s) | 984, 2701 |
| BstAPI | GCANNNNNTGC | 4 cut(s) | 536, 2070, 2591, 2795 |
| BstAUI | TGTACA | 1 cut(s) | 2542 |
| BstBAI | YACGTR | 1 cut(s) | 2577 |
| BstBI | TTCGAA | 1 cut(s) | 229 |
| BstDSI | CCRYGG | 1 cut(s) | 2899 |
| BstMAI | GTCTC | 3 cut(s) | 1291, 2168, 2842 |
| BstNI | CCWGG | 5 cut(s) | 1095, 1277, 2025, 2079, 2278 |
| BstNSI | RCATGY | 3 cut(s) | 175, 1284, 1624 |
| BstSCI | CCNGG | 7 cut(s) | 1093, 1275, 1959, 2023, 2077, 2276, 2781 |
| BstSFI | CTRYAG | 2 cut(s) | 1299, 2134 |
| BstSLI | GKGCMC | 1 cut(s) | 2587 |
| BstV1I | GCAGC | 6 cut(s) | 582, 1572, 1795, 2029, 2778, 2785 |
| BstX2I | RGATCY | 4 cut(s) | 553, 1862, 2027, 2866 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 2149 |
| BstYI | RGATCY | 4 cut(s) | 553, 1862, 2027, 2866 |
| Bsu15I | ATCGAT | 2 cut(s) | 1396, 1594 |
| Bsu36I | CCTNAGG | 1 cut(s) | 291 |
| BsuTUI | ATCGAT | 2 cut(s) | 1396, 1594 |
| BtgI | CCRYGG | 1 cut(s) | 2899 |
| BtgZI | GCGATG | 1 cut(s) | 1930 |
| BtsI | GCAGTG | 3 cut(s) | 495, 537, 624 |
| BtsIMutI | CAGTG | 6 cut(s) | 350, 495, 537, 624, 1314, 2006 |
| CaiI | CAGNNNCTG | 4 cut(s) | 1136, 2042, 2084, 2825 |
| Cfr10I | RCCGGY | 1 cut(s) | 191 |
| ClaI | ATCGAT | 2 cut(s) | 1396, 1594 |
| Csp6I | GTAC | 4 cut(s) | 997, 1026, 1946, 2543 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 963, 998 |
| CviQI | GTAC | 4 cut(s) | 997, 1026, 1946, 2543 |
| DraIII | CACNNNGTG | 1 cut(s) | 2591 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 70 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 70 |
| EaeI | YGGCCR | 3 cut(s) | 157, 193, 2779 |
| Eam1104I | CTCTTC | 2 cut(s) | 984, 2701 |
| EarI | CTCTTC | 2 cut(s) | 984, 2701 |
| EciI | GGCGGA | 1 cut(s) | 47 |
| Ecl136II | GAGCTC | 1 cut(s) | 208 |
| Eco130I | CCWWGG | 2 cut(s) | 82, 1608 |
| Eco147I | AGGCCT | 4 cut(s) | 1492, 1873, 2365, 2527 |
| Eco24I | GRGCYC | 2 cut(s) | 210, 451 |
| Eco32I | GATATC | 2 cut(s) | 307, 478 |
| Eco47I | GGWCC | 9 cut(s) | 220, 659, 738, 1126, 2081, 2285, 2348, 2402, 2510 |
| Eco53kI | GAGCTC | 1 cut(s) | 208 |
| Eco57I | CTGAAG | 6 cut(s) | 1338, 1782, 2304, 2328, 2466, 2490 |
| Eco81I | CCTNAGG | 1 cut(s) | 291 |
| EcoICRI | GAGCTC | 1 cut(s) | 208 |
| EcoO109I | RGGNCCY | 3 cut(s) | 2081, 2285, 2348 |
| EcoRII | CCWGG | 5 cut(s) | 1093, 1275, 2023, 2077, 2276 |
| EcoRV | GATATC | 2 cut(s) | 307, 478 |
| EcoT14I | CCWWGG | 2 cut(s) | 82, 1608 |
| EcoT22I | ATGCAT | 2 cut(s) | 610, 1569 |
| EcoT38I | GRGCYC | 2 cut(s) | 210, 451 |
| ErhI | CCWWGG | 2 cut(s) | 82, 1608 |
| Esp3I | CGTCTC | 1 cut(s) | 2842 |
| FaqI | GGGAC | 3 cut(s) | 272, 1439, 2246 |
| FauI | CCCGC | 2 cut(s) | 125, 259 |
| FauNDI | CATATG | 2 cut(s) | 1108, 1432 |
| FbaI | TGATCA | 2 cut(s) | 1570, 1885 |
| Fnu4HI | GCNGC | 9 cut(s) | 160, 596, 1586, 1784, 2043, 2114, 2164, 2767, 2799 |
| FriOI | GRGCYC | 2 cut(s) | 210, 451 |
| Fsp4HI | GCNGC | 9 cut(s) | 160, 596, 1586, 1784, 2043, 2114, 2164, 2767, 2799 |
| FspBI | CTAG | 9 cut(s) | 540, 579, 1155, 1370, 1488, 1805, 1875, 2117, 2439 |
| GluI | GCNGC | 9 cut(s) | 160, 596, 1586, 1784, 2043, 2114, 2164, 2767, 2799 |
| GsaI | CCCAGC | 2 cut(s) | 1133, 2805 |
| GsuI | CTGGAG | 2 cut(s) | 1236, 1920 |
| HapII | CCGG | 4 cut(s) | 192, 1960, 2782, 2841 |
| HincII | GTYRAC | 2 cut(s) | 505, 2190 |
| HindII | GTYRAC | 2 cut(s) | 505, 2190 |
| HindIII | AAGCTT | 1 cut(s) | 1794 |
| HpaII | CCGG | 4 cut(s) | 192, 1960, 2782, 2841 |
| HphI | GGTGA | 9 cut(s) | 172, 181, 1232, 1349, 1623, 1669, 2371, 2818, 2840 |
| Hpy188I | TCNGA | 9 cut(s) | 561, 588, 763, 915, 957, 967, 1048, 2038, 2757 |
| Hpy188III | TCNNGA | 7 cut(s) | 310, 1215, 1224, 1235, 1472, 1479, 1899 |
| HpyCH4IV | ACGT | 5 cut(s) | 27, 317, 1389, 2058, 2576 |
| HpySE526I | ACGT | 5 cut(s) | 27, 317, 1389, 2058, 2576 |
| Ksp22I | TGATCA | 2 cut(s) | 1570, 1885 |
| LguI | GCTCTTC | 1 cut(s) | 984 |
| LmnI | GCTCC | 4 cut(s) | 205, 274, 1717, 2025 |
| Lsp1109I | GCAGC | 6 cut(s) | 582, 1572, 1795, 2029, 2778, 2785 |
| MaeI | CTAG | 9 cut(s) | 540, 579, 1155, 1370, 1488, 1805, 1875, 2117, 2439 |
| MaeII | ACGT | 5 cut(s) | 27, 317, 1389, 2058, 2576 |
| MaeIII | GTNAC | 8 cut(s) | 178, 187, 632, 865, 1483, 1721, 2047, 2903 |
| MbiI | CCGCTC | 1 cut(s) | 269 |
| MfeI | CAATTG | 1 cut(s) | 1068 |
| MflI | RGATCY | 4 cut(s) | 553, 1862, 2027, 2866 |
| MhlI | GDGCHC | 3 cut(s) | 210, 451, 2587 |
| MlyI | GAGTC | 4 cut(s) | 1240, 1491, 1607, 2170 |
| MmeI | TCCRAC | 6 cut(s) | 282, 1104, 1960, 2274, 2439, 2449 |
| Mph1103I | ATGCAT | 2 cut(s) | 610, 1569 |
| MroXI | GAANNNNTTC | 1 cut(s) | 2209 |
| MseI | TTAA | 2 cut(s) | 1466, 1505 |
| MslI | CAYNNNNRTG | 1 cut(s) | 419 |
| MspA1I | CMGCKG | 2 cut(s) | 1298, 2769 |
| MspI | CCGG | 4 cut(s) | 192, 1960, 2782, 2841 |
| MspR9I | CCNGG | 7 cut(s) | 1095, 1277, 1961, 2025, 2079, 2278, 2783 |
| MunI | CAATTG | 1 cut(s) | 1068 |
| Mva1269I | GAATGC | 2 cut(s) | 2014, 2314 |
| MvaI | CCWGG | 5 cut(s) | 1095, 1277, 2025, 2079, 2278 |
| NciI | CCSGG | 2 cut(s) | 1961, 2783 |
| NdeI | CATATG | 2 cut(s) | 1108, 1432 |
| NlaIV | GGNNCC | 6 cut(s) | 80, 341, 852, 1128, 2021, 2286 |
| NmuCI | GTSAC | 6 cut(s) | 178, 187, 632, 865, 1483, 1721 |
| NsiI | ATGCAT | 2 cut(s) | 610, 1569 |
| NspI | RCATGY | 3 cut(s) | 175, 1284, 1624 |
| NspV | TTCGAA | 1 cut(s) | 229 |
| OliI | CACNNNNGTG | 1 cut(s) | 419 |
| PaeI | GCATGC | 1 cut(s) | 1624 |
| PceI | AGGCCT | 4 cut(s) | 1492, 1873, 2365, 2527 |
| PciSI | GCTCTTC | 1 cut(s) | 984 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 183 |
| PctI | GAATGC | 2 cut(s) | 2014, 2314 |
| PdmI | GAANNNNTTC | 1 cut(s) | 2209 |
| PfeI | GAWTC | 7 cut(s) | 424, 496, 620, 1220, 1403, 1475, 2095 |
| Pfl23II | CGTACG | 1 cut(s) | 1025 |
| PflMI | CCANNNNNTGG | 3 cut(s) | 202, 1136, 1276 |
| PfoI | TCCNGGA | 1 cut(s) | 1093 |
| PkrI | GCNGC | 9 cut(s) | 161, 597, 1587, 1785, 2044, 2115, 2165, 2768, 2800 |
| PleI | GAGTC | 4 cut(s) | 1239, 1490, 1607, 2170 |
| PpsI | GAGTC | 4 cut(s) | 1239, 1490, 1607, 2170 |
| Ppu21I | YACGTR | 1 cut(s) | 2577 |
| PpuMI | RGGWCCY | 3 cut(s) | 2081, 2285, 2348 |
| Psp124BI | GAGCTC | 1 cut(s) | 210 |
| Psp1406I | AACGTT | 1 cut(s) | 2058 |
| Psp5II | RGGWCCY | 3 cut(s) | 2081, 2285, 2348 |
| Psp6I | CCWGG | 5 cut(s) | 1093, 1275, 2023, 2077, 2276 |
| PspFI | CCCAGC | 2 cut(s) | 1129, 2801 |
| PspGI | CCWGG | 5 cut(s) | 1093, 1275, 2023, 2077, 2276 |
| PspLI | CGTACG | 1 cut(s) | 1025 |
| PspN4I | GGNNCC | 6 cut(s) | 80, 341, 852, 1128, 2021, 2286 |
| PspPPI | RGGWCCY | 3 cut(s) | 2081, 2285, 2348 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 2593, 2625 |
| PstNI | CAGNNNCTG | 4 cut(s) | 1136, 2042, 2084, 2825 |
| PsuI | RGATCY | 4 cut(s) | 553, 1862, 2027, 2866 |
| PvuII | CAGCTG | 1 cut(s) | 1298 |
| RsaI | GTAC | 4 cut(s) | 998, 1027, 1947, 2544 |
| RsaNI | GTAC | 4 cut(s) | 997, 1026, 1946, 2543 |
| RseI | CAYNNNNRTG | 1 cut(s) | 419 |
| SacI | GAGCTC | 1 cut(s) | 210 |
| SapI | GCTCTTC | 1 cut(s) | 984 |
| SaqAI | TTAA | 2 cut(s) | 1466, 1505 |
| SatI | GCNGC | 9 cut(s) | 160, 596, 1586, 1784, 2043, 2114, 2164, 2767, 2799 |
| SchI | GAGTC | 4 cut(s) | 1240, 1491, 1607, 2170 |
| ScrFI | CCNGG | 7 cut(s) | 1095, 1277, 1961, 2025, 2079, 2278, 2783 |
| SduI | GDGCHC | 3 cut(s) | 210, 451, 2587 |
| SfcI | CTRYAG | 2 cut(s) | 1299, 2134 |
| SfuI | TTCGAA | 1 cut(s) | 229 |
| SinI | GGWCC | 9 cut(s) | 220, 659, 738, 1126, 2081, 2285, 2348, 2402, 2510 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 419 |
| SmlI | CTYRAG | 1 cut(s) | 1615 |
| SmoI | CTYRAG | 1 cut(s) | 1615 |
| SphI | GCATGC | 1 cut(s) | 1624 |
| SseBI | AGGCCT | 4 cut(s) | 1492, 1873, 2365, 2527 |
| SspMI | CTAG | 9 cut(s) | 540, 579, 1155, 1370, 1488, 1805, 1875, 2117, 2439 |
| SstI | GAGCTC | 1 cut(s) | 210 |
| StuI | AGGCCT | 4 cut(s) | 1492, 1873, 2365, 2527 |
| StyD4I | CCNGG | 7 cut(s) | 1093, 1275, 1959, 2023, 2077, 2276, 2781 |
| StyI | CCWWGG | 2 cut(s) | 82, 1608 |
| TaiI | ACGT | 5 cut(s) | 30, 320, 1392, 2061, 2579 |
| TaqI | TCGA | 7 cut(s) | 210, 229, 363, 1396, 1594, 2353, 2530 |
| TatI | WGTACW | 1 cut(s) | 2542 |
| TauI | GCSGC | 3 cut(s) | 162, 2116, 2166 |
| TfiI | GAWTC | 7 cut(s) | 424, 496, 620, 1220, 1403, 1475, 2095 |
| Tru1I | TTAA | 2 cut(s) | 1466, 1505 |
| Tru9I | TTAA | 2 cut(s) | 1466, 1505 |
| TscAI | CASTG | 6 cut(s) | 350, 495, 537, 631, 1321, 2006 |
| TseFI | GTSAC | 6 cut(s) | 178, 187, 632, 865, 1483, 1721 |
| TseI | GCWGC | 6 cut(s) | 595, 1585, 1783, 2042, 2766, 2798 |
| Tsp45I | GTSAC | 6 cut(s) | 178, 187, 632, 865, 1483, 1721 |
| TspGWI | ACGGA | 5 cut(s) | 175, 1043, 1070, 2207, 2715 |
| TspRI | CASTG | 6 cut(s) | 350, 495, 537, 631, 1321, 2006 |
| Van91I | CCANNNNNTGG | 3 cut(s) | 202, 1136, 1276 |
| VneI | GTGCAC | 1 cut(s) | 2583 |
| VpaK11BI | GGWCC | 9 cut(s) | 220, 659, 738, 1126, 2081, 2285, 2348, 2402, 2510 |
| XapI | RAATTY | 5 cut(s) | 212, 515, 1205, 1969, 2633 |
| XceI | RCATGY | 3 cut(s) | 175, 1284, 1624 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 732, 791 |
| XmnI | GAANNNNTTC | 1 cut(s) | 2209 |
| XspI | CTAG | 9 cut(s) | 540, 579, 1155, 1370, 1488, 1805, 1875, 2117, 2439 |
| Zsp2I | ATGCAT | 2 cut(s) | 610, 1569 |