Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT5G63920 |
| fragaria_vesca | FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 |
| malus_domestica | MD15G1134700.v1.1 |
| prunus_persica | Prupe.4G275500_v2.0.a1 Prupe.4G275500_v2.0.a1 |
| pyrus_communis | pycom15g12120 pycom15g12150 |
| rosa_chinensis | RchiOBHm_Chr2g0122231 |
| rosa_laevigata | RLG00000018671 |
| rosa_roxburghii | Rroxscaffold_2G00121760 |
| rosa_rugosa | Rorug02G0237200 |
| rosa_samantha | Rh2AG296000 Rh2BG304900 Rh2CG283300 Rh2DG320000 |
| rosa_wichuraiana | Rw1G008520 Rw2G023570 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 209 |
| AciI | CCGC | 2 cut(s) | 396, 489 |
| AclWI | GGATC | 2 cut(s) | 452, 488 |
| AcoI | YGGCCR | 1 cut(s) | 406 |
| AcsI | RAATTY | 1 cut(s) | 260 |
| AcuI | CTGAAG | 2 cut(s) | 93, 117 |
| AfaI | GTAC | 1 cut(s) | 171 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 467, 527 |
| AflIII | ACRYGT | 1 cut(s) | 203 |
| AgsI | TTSAA | 1 cut(s) | 11 |
| AluBI | AGCT | 2 cut(s) | 233, 245 |
| AluI | AGCT | 2 cut(s) | 233, 245 |
| Alw26I | GTCTC | 1 cut(s) | 469 |
| AlwI | GGATC | 2 cut(s) | 452, 488 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 452 |
| AoxI | GGCC | 2 cut(s) | 152, 406 |
| ApeKI | GCWGC | 2 cut(s) | 393, 425 |
| ApoI | RAATTY | 1 cut(s) | 260 |
| AspS9I | GGNCC | 2 cut(s) | 29, 137 |
| AsuC2I | CCSGG | 1 cut(s) | 410 |
| AsuHPI | GGTGA | 1 cut(s) | 467 |
| AvaII | GGWCC | 2 cut(s) | 29, 137 |
| BaeGI | GKGCMC | 1 cut(s) | 214 |
| BanI | GGYRCC | 1 cut(s) | 209 |
| BbvCI | CCTCAGC | 1 cut(s) | 364 |
| BbvI | GCAGC | 2 cut(s) | 405, 412 |
| BccI | CCATC | 1 cut(s) | 505 |
| BcnI | CCSGG | 1 cut(s) | 410 |
| BcoDI | GTCTC | 1 cut(s) | 469 |
| BfaI | CTAG | 1 cut(s) | 66 |
| BisI | GCNGC | 2 cut(s) | 394, 426 |
| BlsI | GCNGC | 2 cut(s) | 395, 427 |
| Bme1390I | CCNGG | 1 cut(s) | 410 |
| Bme18I | GGWCC | 2 cut(s) | 29, 137 |
| BmgT120I | GGNCC | 2 cut(s) | 29, 137 |
| BmiI | GGNNCC | 1 cut(s) | 211 |
| BmrFI | CCNGG | 1 cut(s) | 410 |
| BmsI | GCATC | 2 cut(s) | 377, 412 |
| Bpu10I | CCTNAGC | 1 cut(s) | 364 |
| BpuMI | CCSGG | 1 cut(s) | 410 |
| BsaJI | CCNNGG | 2 cut(s) | 409, 526 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 467, 527 |
| Bse118I | RCCGGY | 1 cut(s) | 31 |
| Bse1I | ACTGG | 2 cut(s) | 391, 457 |
| Bse3DI | GCAATG | 1 cut(s) | 42 |
| BseDI | CCNNGG | 2 cut(s) | 409, 526 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 467, 527 |
| BseMI | GCAATG | 1 cut(s) | 42 |
| BseMII | CTCAG | 1 cut(s) | 378 |
| BseNI | ACTGG | 2 cut(s) | 391, 457 |
| BseSI | GKGCMC | 1 cut(s) | 214 |
| BseXI | GCAGC | 2 cut(s) | 405, 412 |
| BsgI | GTGCAG | 1 cut(s) | 435 |
| BshFI | GGCC | 2 cut(s) | 154, 408 |
| BshNI | GGYRCC | 1 cut(s) | 209 |
| BsiSI | CCGG | 2 cut(s) | 32, 409 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 467, 527 |
| BsmAI | GTCTC | 1 cut(s) | 469 |
| BsmBI | CGTCTC | 1 cut(s) | 469 |
| BsnI | GGCC | 2 cut(s) | 154, 408 |
| Bsp1286I | GDGCHC | 1 cut(s) | 214 |
| Bsp1407I | TGTACA | 1 cut(s) | 169 |
| Bsp143I | GATC | 3 cut(s) | 158, 457, 493 |
| BspACI | CCGC | 2 cut(s) | 396, 489 |
| BspANI | GGCC | 2 cut(s) | 154, 408 |
| BspCNI | CTCAG | 1 cut(s) | 377 |
| BspLI | GGNNCC | 1 cut(s) | 211 |
| BspPI | GGATC | 2 cut(s) | 452, 488 |
| BspT107I | GGYRCC | 1 cut(s) | 209 |
| BsrDI | GCAATG | 1 cut(s) | 42 |
| BsrFI | RCCGGY | 1 cut(s) | 31 |
| BsrGI | TGTACA | 1 cut(s) | 169 |
| BsrI | ACTGG | 2 cut(s) | 391, 457 |
| BssAI | RCCGGY | 1 cut(s) | 31 |
| BssECI | CCNNGG | 2 cut(s) | 409, 526 |
| BssMI | GATC | 3 cut(s) | 158, 457, 493 |
| Bst4CI | ACNGT | 2 cut(s) | 89, 527 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 422 |
| BstAUI | TGTACA | 1 cut(s) | 169 |
| BstC8I | GCNNGC | 5 cut(s) | 79, 282, 286, 348, 487 |
| BstDEI | CTNAG | 1 cut(s) | 364 |
| BstDSI | CCRYGG | 1 cut(s) | 526 |
| BstKTI | GATC | 3 cut(s) | 161, 460, 496 |
| BstMAI | GTCTC | 1 cut(s) | 469 |
| BstMBI | GATC | 3 cut(s) | 158, 457, 493 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 218, 422 |
| BstNSI | RCATGY | 1 cut(s) | 207 |
| BstSCI | CCNGG | 1 cut(s) | 408 |
| BstSLI | GKGCMC | 1 cut(s) | 214 |
| BstV1I | GCAGC | 2 cut(s) | 405, 412 |
| BstX2I | RGATCY | 1 cut(s) | 493 |
| BstYI | RGATCY | 1 cut(s) | 493 |
| BsuRI | GGCC | 2 cut(s) | 154, 408 |
| BtgI | CCRYGG | 1 cut(s) | 526 |
| Cac8I | GCNNGC | 5 cut(s) | 79, 282, 286, 348, 487 |
| CaiI | CAGNNNCTG | 1 cut(s) | 452 |
| Cfr10I | RCCGGY | 1 cut(s) | 31 |
| Cfr13I | GGNCC | 2 cut(s) | 29, 137 |
| Csp6I | GTAC | 1 cut(s) | 170 |
| CviAII | CATG | 3 cut(s) | 195, 204, 216 |
| CviJI | RGCY | 5 cut(s) | 154, 233, 245, 312, 408 |
| CviKI_1 | RGCY | 5 cut(s) | 154, 233, 245, 312, 408 |
| CviQI | GTAC | 1 cut(s) | 170 |
| DdeI | CTNAG | 1 cut(s) | 364 |
| DpnI | GATC | 3 cut(s) | 160, 459, 495 |
| DpnII | GATC | 3 cut(s) | 158, 457, 493 |
| EaeI | YGGCCR | 1 cut(s) | 406 |
| Eco147I | AGGCCT | 1 cut(s) | 154 |
| Eco47I | GGWCC | 2 cut(s) | 29, 137 |
| Eco57I | CTGAAG | 2 cut(s) | 93, 117 |
| EcoO109I | RGGNCCY | 1 cut(s) | 137 |
| Esp3I | CGTCTC | 1 cut(s) | 469 |
| FaeI | CATG | 3 cut(s) | 198, 207, 219 |
| FaiI | YATR | 7 cut(s) | 83, 85, 164, 196, 205, 217, 301 |
| FatI | CATG | 3 cut(s) | 194, 203, 215 |
| Fnu4HI | GCNGC | 2 cut(s) | 394, 426 |
| Fsp4HI | GCNGC | 2 cut(s) | 394, 426 |
| FspBI | CTAG | 1 cut(s) | 66 |
| GluI | GCNGC | 2 cut(s) | 394, 426 |
| HaeIII | GGCC | 2 cut(s) | 154, 408 |
| HapII | CCGG | 2 cut(s) | 32, 409 |
| Hin1II | CATG | 3 cut(s) | 198, 207, 219 |
| HinfI | GANTC | 1 cut(s) | 379 |
| HpaII | CCGG | 2 cut(s) | 32, 409 |
| HphI | GGTGA | 1 cut(s) | 467 |
| Hpy166II | GTNNAC | 2 cut(s) | 29, 179 |
| Hpy188I | TCNGA | 1 cut(s) | 384 |
| Hpy8I | GTNNAC | 2 cut(s) | 29, 179 |
| HpyAV | CCTTC | 3 cut(s) | 132, 327, 468 |
| HpyCH4III | ACNGT | 2 cut(s) | 89, 527 |
| HpyCH4V | TGCA | 6 cut(s) | 17, 81, 149, 288, 350, 416 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 218, 422 |
| HpyF3I | CTNAG | 1 cut(s) | 364 |
| Hsp92II | CATG | 3 cut(s) | 198, 207, 219 |
| Kzo9I | GATC | 3 cut(s) | 158, 457, 493 |
| Lsp1109I | GCAGC | 2 cut(s) | 405, 412 |
| LweI | GCATC | 2 cut(s) | 377, 412 |
| MaeI | CTAG | 1 cut(s) | 66 |
| MaeIII | GTNAC | 1 cut(s) | 530 |
| MalI | GATC | 3 cut(s) | 160, 459, 495 |
| MboI | GATC | 3 cut(s) | 158, 457, 493 |
| MboII | GAAGA | 3 cut(s) | 99, 123, 267 |
| MflI | RGATCY | 1 cut(s) | 493 |
| MhlI | GDGCHC | 1 cut(s) | 214 |
| MluCI | AATT | 1 cut(s) | 260 |
| MlyI | GAGTC | 1 cut(s) | 373 |
| MmeI | TCCRAC | 2 cut(s) | 66, 76 |
| MnlI | CCTC | 9 cut(s) | 14, 150, 165, 202, 245, 298, 323, 373, 395 |
| MseI | TTAA | 1 cut(s) | 315 |
| MspA1I | CMGCKG | 1 cut(s) | 396 |
| MspI | CCGG | 2 cut(s) | 32, 409 |
| MspR9I | CCNGG | 1 cut(s) | 410 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 218, 422 |
| NciI | CCSGG | 1 cut(s) | 410 |
| NdeII | GATC | 3 cut(s) | 158, 457, 493 |
| NlaIII | CATG | 3 cut(s) | 198, 207, 219 |
| NlaIV | GGNNCC | 1 cut(s) | 211 |
| NspI | RCATGY | 1 cut(s) | 207 |
| PceI | AGGCCT | 1 cut(s) | 154 |
| PciI | ACATGT | 1 cut(s) | 203 |
| PkrI | GCNGC | 2 cut(s) | 395, 427 |
| PleI | GAGTC | 1 cut(s) | 373 |
| PpsI | GAGTC | 1 cut(s) | 373 |
| PpuMI | RGGWCCY | 1 cut(s) | 137 |
| PscI | ACATGT | 1 cut(s) | 203 |
| Psp5II | RGGWCCY | 1 cut(s) | 137 |
| PspN4I | GGNNCC | 1 cut(s) | 211 |
| PspPI | GGNCC | 2 cut(s) | 29, 137 |
| PspPPI | RGGWCCY | 1 cut(s) | 137 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 220, 252 |
| PstNI | CAGNNNCTG | 1 cut(s) | 452 |
| PsuI | RGATCY | 1 cut(s) | 493 |
| RsaI | GTAC | 1 cut(s) | 171 |
| RsaNI | GTAC | 1 cut(s) | 170 |
| SaqAI | TTAA | 1 cut(s) | 315 |
| SatI | GCNGC | 2 cut(s) | 394, 426 |
| Sau3AI | GATC | 3 cut(s) | 158, 457, 493 |
| Sau96I | GGNCC | 2 cut(s) | 29, 137 |
| SchI | GAGTC | 1 cut(s) | 373 |
| ScrFI | CCNGG | 1 cut(s) | 410 |
| SduI | GDGCHC | 1 cut(s) | 214 |
| SetI | ASST | 6 cut(s) | 139, 194, 235, 247, 406, 479 |
| SfaNI | GCATC | 2 cut(s) | 377, 412 |
| SinI | GGWCC | 2 cut(s) | 29, 137 |
| Sse9I | AATT | 1 cut(s) | 260 |
| SseBI | AGGCCT | 1 cut(s) | 154 |
| SsiI | CCGC | 2 cut(s) | 396, 489 |
| SspMI | CTAG | 1 cut(s) | 66 |
| StuI | AGGCCT | 1 cut(s) | 154 |
| StyD4I | CCNGG | 1 cut(s) | 408 |
| TaaI | ACNGT | 2 cut(s) | 89, 527 |
| TaqI | TCGA | 1 cut(s) | 157 |
| TasI | AATT | 1 cut(s) | 260 |
| TatI | WGTACW | 1 cut(s) | 169 |
| Tru1I | TTAA | 1 cut(s) | 315 |
| Tru9I | TTAA | 1 cut(s) | 315 |
| TseI | GCWGC | 2 cut(s) | 393, 425 |
| TspDTI | ATGAA | 2 cut(s) | 17, 179 |
| VpaK11BI | GGWCC | 2 cut(s) | 29, 137 |
| XapI | RAATTY | 1 cut(s) | 260 |
| XceI | RCATGY | 1 cut(s) | 207 |
| XspI | CTAG | 1 cut(s) | 66 |