Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT5G63920 |
| fragaria_vesca | FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 |
| malus_domestica | MD15G1134700.v1.1 |
| prunus_persica | Prupe.4G275500_v2.0.a1 Prupe.4G275500_v2.0.a1 |
| pyrus_communis | pycom15g12120 pycom15g12150 |
| rosa_chinensis | RchiOBHm_Chr2g0122231 |
| rosa_laevigata | RLG00000018671 |
| rosa_roxburghii | Rroxscaffold_2G00121760 |
| rosa_rugosa | Rorug02G0237200 |
| rosa_samantha | Rh2AG296000 Rh2BG304900 Rh2CG283300 Rh2DG320000 |
| rosa_wichuraiana | Rw1G008520 Rw2G023570 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 937 |
| AccB1I | GGYRCC | 2 cut(s) | 878, 893 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1022 |
| AccII | CGCG | 1 cut(s) | 885 |
| AciI | CCGC | 9 cut(s) | 278, 320, 501, 540, 747, 837, 883, 885, 942 |
| AclI | AACGTT | 1 cut(s) | 861 |
| AclWI | GGATC | 3 cut(s) | 376, 947, 983 |
| AcsI | RAATTY | 2 cut(s) | 310, 767 |
| AcuI | CTGAAG | 3 cut(s) | 123, 600, 624 |
| AdeI | CACNNNGTG | 1 cut(s) | 683 |
| AfaI | GTAC | 1 cut(s) | 706 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 277, 787, 962, 1022 |
| AflIII | ACRYGT | 1 cut(s) | 710 |
| AgsI | TTSAA | 2 cut(s) | 179, 440 |
| AjnI | CCWGG | 5 cut(s) | 214, 270, 364, 418, 572 |
| AluBI | AGCT | 3 cut(s) | 50, 101, 386 |
| AluI | AGCT | 3 cut(s) | 50, 101, 386 |
| Alw21I | GWGCWC | 2 cut(s) | 721, 728 |
| Alw26I | GTCTC | 1 cut(s) | 703 |
| Alw44I | GTGCAC | 2 cut(s) | 717, 724 |
| AlwI | GGATC | 3 cut(s) | 376, 947, 983 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 425 |
| Ama87I | CYCGRG | 1 cut(s) | 897 |
| AoxI | GGCC | 2 cut(s) | 643, 659 |
| ApaLI | GTGCAC | 2 cut(s) | 717, 724 |
| ApeKI | GCWGC | 4 cut(s) | 124, 229, 383, 521 |
| ApoI | RAATTY | 2 cut(s) | 310, 767 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 508 |
| AspS9I | GGNCC | 2 cut(s) | 422, 536 |
| AsuC2I | CCSGG | 2 cut(s) | 898, 899 |
| AsuHPI | GGTGA | 1 cut(s) | 962 |
| AvaI | CYCGRG | 1 cut(s) | 897 |
| AvaII | GGWCC | 2 cut(s) | 422, 536 |
| BaeGI | GKGCMC | 4 cut(s) | 721, 728, 883, 898 |
| BanI | GGYRCC | 2 cut(s) | 878, 893 |
| Bbv12I | GWGCWC | 2 cut(s) | 721, 728 |
| BbvI | GCAGC | 4 cut(s) | 136, 241, 395, 533 |
| BccI | CCATC | 2 cut(s) | 299, 654 |
| BciT130I | CCWGG | 5 cut(s) | 216, 272, 366, 420, 574 |
| BcnI | CCSGG | 2 cut(s) | 898, 899 |
| BcoDI | GTCTC | 1 cut(s) | 703 |
| BisI | GCNGC | 5 cut(s) | 125, 230, 384, 522, 886 |
| BlsI | GCNGC | 5 cut(s) | 126, 231, 385, 523, 887 |
| Bme1390I | CCNGG | 7 cut(s) | 216, 272, 366, 420, 574, 898, 899 |
| Bme18I | GGWCC | 2 cut(s) | 422, 536 |
| BmeT110I | CYCGRG | 1 cut(s) | 897 |
| BmgT120I | GGNCC | 2 cut(s) | 422, 536 |
| BmiI | GGNNCC | 4 cut(s) | 362, 538, 880, 895 |
| BmrFI | CCNGG | 7 cut(s) | 216, 272, 366, 420, 574, 898, 899 |
| BmsI | GCATC | 3 cut(s) | 139, 245, 314 |
| BpmI | CTGGAG | 2 cut(s) | 261, 402 |
| BpuMI | CCSGG | 2 cut(s) | 898, 899 |
| BsaBI | GATNNNNATC | 1 cut(s) | 373 |
| BsaJI | CCNNGG | 6 cut(s) | 183, 364, 883, 897, 898, 1021 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 277, 787, 962, 1022 |
| Bse1I | ACTGG | 4 cut(s) | 511, 601, 928, 952 |
| Bse3DI | GCAATG | 2 cut(s) | 559, 832 |
| Bse8I | GATNNNNATC | 1 cut(s) | 373 |
| BseBI | CCWGG | 5 cut(s) | 216, 272, 366, 420, 574 |
| BseDI | CCNNGG | 6 cut(s) | 183, 364, 883, 897, 898, 1021 |
| BseGI | GGATG | 3 cut(s) | 310, 329, 545 |
| BseJI | GATNNNNATC | 1 cut(s) | 373 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 277, 787, 962, 1022 |
| BseMI | GCAATG | 2 cut(s) | 559, 832 |
| BseMII | CTCAG | 1 cut(s) | 353 |
| BseNI | ACTGG | 4 cut(s) | 511, 601, 928, 952 |
| BseSI | GKGCMC | 4 cut(s) | 721, 728, 883, 898 |
| BseXI | GCAGC | 4 cut(s) | 136, 241, 395, 533 |
| BsgI | GTGCAG | 1 cut(s) | 924 |
| Bsh1236I | CGCG | 1 cut(s) | 885 |
| BshFI | GGCC | 2 cut(s) | 645, 661 |
| BshNI | GGYRCC | 2 cut(s) | 878, 893 |
| BsiHKAI | GWGCWC | 2 cut(s) | 721, 728 |
| BsiHKCI | CYCGRG | 1 cut(s) | 897 |
| BsiSI | CCGG | 1 cut(s) | 898 |
| BslFI | GGGAC | 1 cut(s) | 943 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 277, 787, 962, 1022 |
| BsmAI | GTCTC | 1 cut(s) | 703 |
| BsmFI | GGGAC | 1 cut(s) | 943 |
| BsmI | GAATGC | 3 cut(s) | 355, 610, 679 |
| BsnI | GGCC | 2 cut(s) | 645, 661 |
| BsoBI | CYCGRG | 1 cut(s) | 897 |
| Bsp1286I | GDGCHC | 4 cut(s) | 721, 728, 883, 898 |
| Bsp143I | GATC | 4 cut(s) | 203, 368, 952, 988 |
| Bsp19I | CCATGG | 1 cut(s) | 183 |
| BspACI | CCGC | 9 cut(s) | 278, 320, 501, 540, 747, 837, 883, 885, 942 |
| BspANI | GGCC | 2 cut(s) | 645, 661 |
| BspCNI | CTCAG | 1 cut(s) | 352 |
| BspFNI | CGCG | 1 cut(s) | 885 |
| BspLI | GGNNCC | 4 cut(s) | 362, 538, 880, 895 |
| BspPI | GGATC | 3 cut(s) | 376, 947, 983 |
| BspT107I | GGYRCC | 2 cut(s) | 878, 893 |
| BsrDI | GCAATG | 2 cut(s) | 559, 832 |
| BsrI | ACTGG | 4 cut(s) | 511, 601, 928, 952 |
| BssECI | CCNNGG | 6 cut(s) | 183, 364, 883, 897, 898, 1021 |
| BssMI | GATC | 4 cut(s) | 203, 368, 952, 988 |
| BssT1I | CCWWGG | 1 cut(s) | 183 |
| Bst2UI | CCWGG | 5 cut(s) | 216, 272, 366, 420, 574 |
| Bst4CI | ACNGT | 3 cut(s) | 486, 723, 1022 |
| Bst6I | CTCTTC | 1 cut(s) | 835 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 411, 594, 917 |
| BstC8I | GCNNGC | 6 cut(s) | 122, 139, 234, 586, 855, 883 |
| BstDEI | CTNAG | 2 cut(s) | 44, 339 |
| BstDSI | CCRYGG | 3 cut(s) | 183, 883, 1021 |
| BstF5I | GGATG | 3 cut(s) | 310, 329, 545 |
| BstFNI | CGCG | 1 cut(s) | 885 |
| BstKTI | GATC | 4 cut(s) | 206, 371, 955, 991 |
| BstMAI | GTCTC | 1 cut(s) | 703 |
| BstMBI | GATC | 4 cut(s) | 203, 368, 952, 988 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 411, 594, 746, 902, 917 |
| BstNI | CCWGG | 5 cut(s) | 216, 272, 366, 420, 574 |
| BstNSI | RCATGY | 3 cut(s) | 236, 714, 920 |
| BstSCI | CCNGG | 7 cut(s) | 214, 270, 364, 418, 572, 896, 897 |
| BstSLI | GKGCMC | 4 cut(s) | 721, 728, 883, 898 |
| BstUI | CGCG | 1 cut(s) | 885 |
| BstV1I | GCAGC | 4 cut(s) | 136, 241, 395, 533 |
| BstX2I | RGATCY | 2 cut(s) | 368, 988 |
| BstYI | RGATCY | 2 cut(s) | 368, 988 |
| BsuRI | GGCC | 2 cut(s) | 645, 661 |
| BtgI | CCRYGG | 3 cut(s) | 183, 883, 1021 |
| BtsCI | GGATG | 3 cut(s) | 310, 329, 545 |
| BtsI | GCAGTG | 2 cut(s) | 265, 874 |
| BtsIMutI | CAGTG | 3 cut(s) | 265, 347, 874 |
| Cac8I | GCNNGC | 6 cut(s) | 122, 139, 234, 586, 855, 883 |
| CaiI | CAGNNNCTG | 1 cut(s) | 425 |
| Cfr13I | GGNCC | 2 cut(s) | 422, 536 |
| Cfr42I | CCGCGG | 1 cut(s) | 886 |
| Cfr9I | CCCGGG | 1 cut(s) | 897 |
| CsiI | ACCWGGT | 1 cut(s) | 214 |
| Csp6I | GTAC | 1 cut(s) | 705 |
| CviJI | RGCY | 9 cut(s) | 50, 101, 137, 157, 182, 361, 386, 645, 661 |
| CviKI_1 | RGCY | 9 cut(s) | 50, 101, 137, 157, 182, 361, 386, 645, 661 |
| CviQI | GTAC | 1 cut(s) | 705 |
| DdeI | CTNAG | 2 cut(s) | 44, 339 |
| DpnI | GATC | 4 cut(s) | 205, 370, 954, 990 |
| DpnII | GATC | 4 cut(s) | 203, 368, 952, 988 |
| DraIII | CACNNNGTG | 1 cut(s) | 683 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 937 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 937 |
| Eam1104I | CTCTTC | 1 cut(s) | 835 |
| EarI | CTCTTC | 1 cut(s) | 835 |
| EciI | GGCGGA | 1 cut(s) | 852 |
| Eco130I | CCWWGG | 1 cut(s) | 183 |
| Eco147I | AGGCCT | 1 cut(s) | 661 |
| Eco47I | GGWCC | 2 cut(s) | 422, 536 |
| Eco57I | CTGAAG | 3 cut(s) | 123, 600, 624 |
| Eco88I | CYCGRG | 1 cut(s) | 897 |
| EcoO109I | RGGNCCY | 2 cut(s) | 422, 536 |
| EcoRII | CCWGG | 5 cut(s) | 214, 270, 364, 418, 572 |
| EcoT14I | CCWWGG | 1 cut(s) | 183 |
| EcoT22I | ATGCAT | 2 cut(s) | 238, 329 |
| ErhI | CCWWGG | 1 cut(s) | 183 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 156, 188 |
| FaqI | GGGAC | 1 cut(s) | 943 |
| FauI | CCCGC | 3 cut(s) | 313, 547, 890 |
| Fnu4HI | GCNGC | 5 cut(s) | 125, 230, 384, 522, 886 |
| FokI | GGATG | 3 cut(s) | 317, 336, 532 |
| Fsp4HI | GCNGC | 5 cut(s) | 125, 230, 384, 522, 886 |
| GluI | GCNGC | 5 cut(s) | 125, 230, 384, 522, 886 |
| GsuI | CTGGAG | 2 cut(s) | 261, 402 |
| HaeIII | GGCC | 2 cut(s) | 645, 661 |
| HapII | CCGG | 1 cut(s) | 898 |
| HinfI | GANTC | 2 cut(s) | 436, 475 |
| HpaII | CCGG | 1 cut(s) | 898 |
| HphI | GGTGA | 1 cut(s) | 962 |
| Hpy166II | GTNNAC | 4 cut(s) | 263, 430, 719, 726 |
| Hpy188I | TCNGA | 2 cut(s) | 294, 379 |
| Hpy188III | TCNNGA | 3 cut(s) | 31, 240, 302 |
| Hpy8I | GTNNAC | 4 cut(s) | 263, 430, 719, 726 |
| Hpy99I | CGWCG | 2 cut(s) | 258, 787 |
| HpyAV | CCTTC | 3 cut(s) | 77, 639, 963 |
| HpyCH4III | ACNGT | 3 cut(s) | 486, 723, 1022 |
| HpyCH4IV | ACGT | 2 cut(s) | 819, 861 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 411, 594, 746, 902, 917 |
| HpyF3I | CTNAG | 2 cut(s) | 44, 339 |
| HpySE526I | ACGT | 2 cut(s) | 819, 861 |
| KspI | CCGCGG | 1 cut(s) | 886 |
| Kzo9I | GATC | 4 cut(s) | 203, 368, 952, 988 |
| LmnI | GCTCC | 3 cut(s) | 58, 366, 908 |
| Lsp1109I | GCAGC | 4 cut(s) | 136, 241, 395, 533 |
| LweI | GCATC | 3 cut(s) | 139, 245, 314 |
| MabI | ACCWGGT | 1 cut(s) | 214 |
| MaeII | ACGT | 2 cut(s) | 819, 861 |
| MaeIII | GTNAC | 4 cut(s) | 62, 388, 467, 993 |
| MalI | GATC | 4 cut(s) | 205, 370, 954, 990 |
| MboI | GATC | 4 cut(s) | 203, 368, 952, 988 |
| MboII | GAAGA | 5 cut(s) | 470, 606, 630, 774, 852 |
| MflI | RGATCY | 2 cut(s) | 368, 988 |
| MhlI | GDGCHC | 4 cut(s) | 721, 728, 883, 898 |
| MluCI | AATT | 4 cut(s) | 310, 440, 767, 958 |
| MnlI | CCTC | 9 cut(s) | 147, 348, 521, 527, 672, 752, 805, 836, 884 |
| Mph1103I | ATGCAT | 2 cut(s) | 238, 329 |
| MroXI | GAANNNNTTC | 1 cut(s) | 508 |
| MseI | TTAA | 1 cut(s) | 488 |
| MslI | CAYNNNNRTG | 2 cut(s) | 188, 651 |
| MspA1I | CMGCKG | 2 cut(s) | 386, 885 |
| MspI | CCGG | 1 cut(s) | 898 |
| MspR9I | CCNGG | 7 cut(s) | 216, 272, 366, 420, 574, 898, 899 |
| Mva1269I | GAATGC | 3 cut(s) | 355, 610, 679 |
| MvaI | CCWGG | 5 cut(s) | 216, 272, 366, 420, 574 |
| MvnI | CGCG | 1 cut(s) | 885 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 411, 594, 746, 902, 917 |
| NciI | CCSGG | 2 cut(s) | 898, 899 |
| NcoI | CCATGG | 1 cut(s) | 183 |
| NdeII | GATC | 4 cut(s) | 203, 368, 952, 988 |
| NlaIV | GGNNCC | 4 cut(s) | 362, 538, 880, 895 |
| NmuCI | GTSAC | 3 cut(s) | 62, 467, 993 |
| NsiI | ATGCAT | 2 cut(s) | 238, 329 |
| NspI | RCATGY | 3 cut(s) | 236, 714, 920 |
| PaeI | GCATGC | 1 cut(s) | 236 |
| PceI | AGGCCT | 1 cut(s) | 661 |
| PciI | ACATGT | 1 cut(s) | 710 |
| PctI | GAATGC | 3 cut(s) | 355, 610, 679 |
| PdmI | GAANNNNTTC | 1 cut(s) | 508 |
| PfeI | GAWTC | 2 cut(s) | 436, 475 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1022 |
| PkrI | GCNGC | 5 cut(s) | 126, 231, 385, 523, 887 |
| PpuMI | RGGWCCY | 2 cut(s) | 422, 536 |
| PscI | ACATGT | 1 cut(s) | 710 |
| Psp1406I | AACGTT | 1 cut(s) | 861 |
| Psp5II | RGGWCCY | 2 cut(s) | 422, 536 |
| Psp6I | CCWGG | 5 cut(s) | 214, 270, 364, 418, 572 |
| PspGI | CCWGG | 5 cut(s) | 214, 270, 364, 418, 572 |
| PspN4I | GGNNCC | 4 cut(s) | 362, 538, 880, 895 |
| PspPI | GGNCC | 2 cut(s) | 422, 536 |
| PspPPI | RGGWCCY | 2 cut(s) | 422, 536 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 727, 759 |
| PstNI | CAGNNNCTG | 1 cut(s) | 425 |
| PsuI | RGATCY | 2 cut(s) | 368, 988 |
| PvuII | CAGCTG | 1 cut(s) | 386 |
| RsaI | GTAC | 1 cut(s) | 706 |
| RsaNI | GTAC | 1 cut(s) | 705 |
| RseI | CAYNNNNRTG | 2 cut(s) | 188, 651 |
| SacII | CCGCGG | 1 cut(s) | 886 |
| SaqAI | TTAA | 1 cut(s) | 488 |
| SatI | GCNGC | 5 cut(s) | 125, 230, 384, 522, 886 |
| Sau3AI | GATC | 4 cut(s) | 203, 368, 952, 988 |
| Sau96I | GGNCC | 2 cut(s) | 422, 536 |
| ScrFI | CCNGG | 7 cut(s) | 216, 272, 366, 420, 574, 898, 899 |
| SduI | GDGCHC | 4 cut(s) | 721, 728, 883, 898 |
| SexAI | ACCWGGT | 1 cut(s) | 214 |
| SfaNI | GCATC | 3 cut(s) | 139, 245, 314 |
| Sfr303I | CCGCGG | 1 cut(s) | 886 |
| SgrBI | CCGCGG | 1 cut(s) | 886 |
| SinI | GGWCC | 2 cut(s) | 422, 536 |
| SmaI | CCCGGG | 1 cut(s) | 899 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 188, 651 |
| SphI | GCATGC | 1 cut(s) | 236 |
| Sse9I | AATT | 4 cut(s) | 310, 440, 767, 958 |
| SseBI | AGGCCT | 1 cut(s) | 661 |
| SsiI | CCGC | 9 cut(s) | 278, 320, 501, 540, 747, 837, 883, 885, 942 |
| StuI | AGGCCT | 1 cut(s) | 661 |
| StyD4I | CCNGG | 7 cut(s) | 214, 270, 364, 418, 572, 896, 897 |
| StyI | CCWWGG | 1 cut(s) | 183 |
| TaaI | ACNGT | 3 cut(s) | 486, 723, 1022 |
| TaiI | ACGT | 2 cut(s) | 822, 864 |
| TaqI | TCGA | 4 cut(s) | 253, 481, 649, 664 |
| TaqII | GACCGA | 1 cut(s) | 504 |
| TasI | AATT | 4 cut(s) | 310, 440, 767, 958 |
| TatI | WGTACW | 1 cut(s) | 704 |
| TauI | GCSGC | 1 cut(s) | 888 |
| TfiI | GAWTC | 2 cut(s) | 436, 475 |
| Tru1I | TTAA | 1 cut(s) | 488 |
| Tru9I | TTAA | 1 cut(s) | 488 |
| TscAI | CASTG | 3 cut(s) | 272, 347, 874 |
| TseFI | GTSAC | 3 cut(s) | 62, 467, 993 |
| TseI | GCWGC | 4 cut(s) | 124, 229, 383, 521 |
| Tsp45I | GTSAC | 3 cut(s) | 62, 467, 993 |
| TspDTI | ATGAA | 4 cut(s) | 83, 146, 238, 686 |
| TspGWI | ACGGA | 1 cut(s) | 981 |
| TspMI | CCCGGG | 1 cut(s) | 897 |
| TspRI | CASTG | 3 cut(s) | 272, 347, 874 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1022 |
| VneI | GTGCAC | 2 cut(s) | 717, 724 |
| VpaK11BI | GGWCC | 2 cut(s) | 422, 536 |
| XapI | RAATTY | 2 cut(s) | 310, 767 |
| XceI | RCATGY | 3 cut(s) | 236, 714, 920 |
| XmaI | CCCGGG | 1 cut(s) | 897 |
| XmnI | GAANNNNTTC | 1 cut(s) | 508 |
| Zsp2I | ATGCAT | 2 cut(s) | 238, 329 |