Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT5G63920 |
| fragaria_vesca | FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 |
| malus_domestica | MD15G1134700.v1.1 |
| prunus_persica | Prupe.4G275500_v2.0.a1 Prupe.4G275500_v2.0.a1 |
| pyrus_communis | pycom15g12120 pycom15g12150 |
| rosa_chinensis | RchiOBHm_Chr2g0122231 |
| rosa_laevigata | RLG00000018671 |
| rosa_roxburghii | Rroxscaffold_2G00121760 |
| rosa_rugosa | Rorug02G0237200 |
| rosa_samantha | Rh2AG296000 Rh2BG304900 Rh2CG283300 Rh2DG320000 |
| rosa_wichuraiana | Rw1G008520 Rw2G023570 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 1799 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 196, 1270 |
| AccII | CGCG | 2 cut(s) | 9, 112 |
| AciI | CCGC | 9 cut(s) | 9, 32, 217, 223, 271, 524, 852, 1931, 1973 |
| AclI | AACGTT | 1 cut(s) | 1457 |
| AclWI | GGATC | 4 cut(s) | 236, 839, 852, 1105 |
| AcoI | YGGCCR | 2 cut(s) | 187, 790 |
| AcsI | RAATTY | 4 cut(s) | 206, 725, 1199, 1963 |
| AcuI | CTGAAG | 2 cut(s) | 908, 1776 |
| AfaI | GTAC | 2 cut(s) | 1068, 1546 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 196, 438, 674, 1094, 1130, 1154, 1270, 1295, 1930 |
| AflIII | ACRYGT | 2 cut(s) | 1616, 2018 |
| AgsI | TTSAA | 7 cut(s) | 136, 392, 910, 926, 1316, 1397, 1832 |
| AjnI | CCWGG | 5 cut(s) | 258, 572, 1087, 1867, 1923 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1186, 1218 |
| Alw21I | GWGCWC | 1 cut(s) | 204 |
| Alw26I | GTCTC | 4 cut(s) | 9, 78, 1019, 1285 |
| AlwI | GGATC | 4 cut(s) | 236, 839, 852, 1105 |
| Ama87I | CYCGRG | 2 cut(s) | 75, 719 |
| AoxI | GGCC | 6 cut(s) | 79, 103, 187, 790, 1173, 1484 |
| ApeKI | GCWGC | 3 cut(s) | 153, 1777, 1882 |
| ApoI | RAATTY | 4 cut(s) | 206, 725, 1199, 1963 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 56, 88 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 955 |
| AspLEI | GCGC | 1 cut(s) | 114 |
| AspS9I | GGNCC | 5 cut(s) | 79, 653, 732, 1120, 1174 |
| AsuHPI | GGTGA | 7 cut(s) | 166, 175, 1181, 1617, 1652, 1663, 1727 |
| AsuII | TTCGAA | 3 cut(s) | 91, 210, 949 |
| AvaI | CYCGRG | 2 cut(s) | 75, 719 |
| AvaII | GGWCC | 3 cut(s) | 653, 732, 1120 |
| AxyI | CCTNAGG | 2 cut(s) | 285, 1992 |
| BalI | TGGCCA | 1 cut(s) | 792 |
| BamHI | GGATCC | 1 cut(s) | 844 |
| BanII | GRGCYC | 3 cut(s) | 204, 445, 720 |
| BbsI | GAAGAC | 1 cut(s) | 219 |
| Bbv12I | GWGCWC | 1 cut(s) | 204 |
| BbvI | GCAGC | 3 cut(s) | 140, 1789, 1894 |
| BccI | CCATC | 8 cut(s) | 152, 483, 1025, 1086, 1185, 1215, 1648, 1952 |
| BceAI | ACGGC | 1 cut(s) | 25 |
| BciT130I | CCWGG | 5 cut(s) | 260, 574, 1089, 1869, 1925 |
| BciVI | GTATCC | 1 cut(s) | 903 |
| BclI | TGATCA | 2 cut(s) | 802, 1564 |
| BcoDI | GTCTC | 4 cut(s) | 9, 78, 1019, 1285 |
| BfaI | CTAG | 4 cut(s) | 327, 534, 1149, 1482 |
| BfmI | CTRYAG | 1 cut(s) | 1293 |
| BfuAI | ACCTGC | 1 cut(s) | 1799 |
| BfuI | GTATCC | 1 cut(s) | 903 |
| BglII | AGATCT | 1 cut(s) | 547 |
| BisI | GCNGC | 4 cut(s) | 10, 154, 1778, 1883 |
| BlsI | GCNGC | 4 cut(s) | 11, 155, 1779, 1884 |
| Bme1390I | CCNGG | 5 cut(s) | 260, 574, 1089, 1869, 1925 |
| Bme18I | GGWCC | 3 cut(s) | 653, 732, 1120 |
| BmeT110I | CYCGRG | 2 cut(s) | 75, 719 |
| BmgT120I | GGNCC | 5 cut(s) | 79, 653, 732, 1120, 1174 |
| BmiI | GGNNCC | 4 cut(s) | 80, 264, 846, 1122 |
| BmrFI | CCNGG | 5 cut(s) | 260, 574, 1089, 1869, 1925 |
| BpiI | GAAGAC | 1 cut(s) | 219 |
| BpmI | CTGGAG | 2 cut(s) | 1230, 1914 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1943 |
| Bpu14I | TTCGAA | 3 cut(s) | 91, 210, 949 |
| BsaBI | GATNNNNATC | 4 cut(s) | 474, 1096, 1236, 1386 |
| BsaI | GGTCTC | 1 cut(s) | 1019 |
| BsaJI | CCNNGG | 4 cut(s) | 82, 720, 1602, 1836 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 26, 56, 692, 722, 1471, 1501 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 196, 438, 674, 1094, 1130, 1154, 1270, 1295, 1930 |
| Bse118I | RCCGGY | 1 cut(s) | 185 |
| Bse1I | ACTGG | 2 cut(s) | 337, 1368 |
| Bse21I | CCTNAGG | 2 cut(s) | 285, 1992 |
| Bse3DI | GCAATG | 1 cut(s) | 381 |
| Bse8I | GATNNNNATC | 4 cut(s) | 474, 1096, 1236, 1386 |
| BseBI | CCWGG | 5 cut(s) | 260, 574, 1089, 1869, 1925 |
| BseDI | CCNNGG | 4 cut(s) | 82, 720, 1602, 1836 |
| BseJI | GATNNNNATC | 4 cut(s) | 474, 1096, 1236, 1386 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 196, 438, 674, 1094, 1130, 1154, 1270, 1295, 1930 |
| BseMI | GCAATG | 1 cut(s) | 381 |
| BseMII | CTCAG | 4 cut(s) | 276, 465, 584, 2006 |
| BseNI | ACTGG | 2 cut(s) | 337, 1368 |
| BseRI | GAGGAG | 1 cut(s) | 332 |
| BseXI | GCAGC | 3 cut(s) | 140, 1789, 1894 |
| BseYI | CCCAGC | 1 cut(s) | 1516 |
| BsgI | GTGCAG | 2 cut(s) | 391, 501 |
| Bsh1236I | CGCG | 2 cut(s) | 9, 112 |
| Bsh1285I | CGRYCG | 1 cut(s) | 110 |
| BshFI | GGCC | 6 cut(s) | 81, 105, 189, 792, 1175, 1486 |
| BsiEI | CGRYCG | 1 cut(s) | 110 |
| BsiHKAI | GWGCWC | 1 cut(s) | 204 |
| BsiHKCI | CYCGRG | 2 cut(s) | 75, 719 |
| BsiSI | CCGG | 1 cut(s) | 186 |
| BslFI | GGGAC | 1 cut(s) | 266 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 196, 438, 674, 1094, 1130, 1154, 1270, 1295, 1930 |
| BsmAI | GTCTC | 4 cut(s) | 9, 78, 1019, 1285 |
| BsmFI | GGGAC | 1 cut(s) | 266 |
| BsnI | GGCC | 6 cut(s) | 81, 105, 189, 792, 1175, 1486 |
| Bso31I | GGTCTC | 1 cut(s) | 1019 |
| BsoBI | CYCGRG | 2 cut(s) | 75, 719 |
| Bsp119I | TTCGAA | 3 cut(s) | 91, 210, 949 |
| Bsp1286I | GDGCHC | 3 cut(s) | 204, 445, 720 |
| Bsp1407I | TGTACA | 1 cut(s) | 1066 |
| Bsp19I | CCATGG | 1 cut(s) | 1836 |
| BspACI | CCGC | 9 cut(s) | 9, 32, 217, 223, 271, 524, 852, 1931, 1973 |
| BspANI | GGCC | 6 cut(s) | 81, 105, 189, 792, 1175, 1486 |
| BspCNI | CTCAG | 4 cut(s) | 277, 464, 585, 2005 |
| BspFNI | CGCG | 2 cut(s) | 9, 112 |
| BspLI | GGNNCC | 4 cut(s) | 80, 264, 846, 1122 |
| BspMI | ACCTGC | 1 cut(s) | 1799 |
| BspPI | GGATC | 4 cut(s) | 236, 839, 852, 1105 |
| BspQI | GCTCTTC | 2 cut(s) | 176, 978 |
| BspT104I | TTCGAA | 3 cut(s) | 91, 210, 949 |
| BspTNI | GGTCTC | 1 cut(s) | 1019 |
| BsrDI | GCAATG | 1 cut(s) | 381 |
| BsrFI | RCCGGY | 1 cut(s) | 185 |
| BsrGI | TGTACA | 1 cut(s) | 1066 |
| BsrI | ACTGG | 2 cut(s) | 337, 1368 |
| BssAI | RCCGGY | 1 cut(s) | 185 |
| BssECI | CCNNGG | 4 cut(s) | 82, 720, 1602, 1836 |
| BssT1I | CCWWGG | 3 cut(s) | 82, 1602, 1836 |
| Bst2UI | CCWGG | 5 cut(s) | 260, 574, 1089, 1869, 1925 |
| Bst4CI | ACNGT | 9 cut(s) | 72, 362, 693, 708, 743, 859, 1245, 1312, 1443 |
| Bst6I | CTCTTC | 3 cut(s) | 176, 260, 978 |
| BstAUI | TGTACA | 1 cut(s) | 1066 |
| BstBI | TTCGAA | 3 cut(s) | 91, 210, 949 |
| BstC8I | GCNNGC | 6 cut(s) | 933, 1059, 1290, 1330, 1775, 1887 |
| BstDEI | CTNAG | 8 cut(s) | 285, 451, 593, 644, 1378, 1697, 1943, 1992 |
| BstDSI | CCRYGG | 1 cut(s) | 1836 |
| BstFNI | CGCG | 2 cut(s) | 9, 112 |
| BstHHI | GCGC | 1 cut(s) | 114 |
| BstMAI | GTCTC | 4 cut(s) | 9, 78, 1019, 1285 |
| BstMCI | CGRYCG | 1 cut(s) | 110 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 111, 159, 530, 928, 1298 |
| BstNI | CCWGG | 5 cut(s) | 260, 574, 1089, 1869, 1925 |
| BstNSI | RCATGY | 4 cut(s) | 1061, 1278, 1889, 2022 |
| BstSCI | CCNGG | 5 cut(s) | 258, 572, 1087, 1867, 1923 |
| BstSFI | CTRYAG | 1 cut(s) | 1293 |
| BstUI | CGCG | 2 cut(s) | 9, 112 |
| BstV1I | GCAGC | 3 cut(s) | 140, 1789, 1894 |
| BstV2I | GAAGAC | 1 cut(s) | 219 |
| BstX2I | RGATCY | 2 cut(s) | 547, 844 |
| BstYI | RGATCY | 2 cut(s) | 547, 844 |
| Bsu36I | CCTNAGG | 2 cut(s) | 285, 1992 |
| BsuI | GTATCC | 1 cut(s) | 903 |
| BsuRI | GGCC | 6 cut(s) | 81, 105, 189, 792, 1175, 1486 |
| BtgI | CCRYGG | 1 cut(s) | 1836 |
| BtsI | GCAGTG | 3 cut(s) | 489, 1412, 1918 |
| BtsIMutI | CAGTG | 4 cut(s) | 344, 489, 1412, 1918 |
| BveI | ACCTGC | 1 cut(s) | 1799 |
| Cac8I | GCNNGC | 6 cut(s) | 933, 1059, 1290, 1330, 1775, 1887 |
| CfoI | GCGC | 1 cut(s) | 114 |
| Cfr10I | RCCGGY | 1 cut(s) | 185 |
| Cfr13I | GGNCC | 5 cut(s) | 79, 653, 732, 1120, 1174 |
| CseI | GACGC | 1 cut(s) | 924 |
| CsiI | ACCWGGT | 2 cut(s) | 572, 1867 |
| Csp6I | GTAC | 2 cut(s) | 1067, 1545 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1479, 1514 |
| CviQI | GTAC | 2 cut(s) | 1067, 1545 |
| DdeI | CTNAG | 8 cut(s) | 285, 451, 593, 644, 1378, 1697, 1943, 1992 |
| EaeI | YGGCCR | 2 cut(s) | 187, 790 |
| Eam1104I | CTCTTC | 3 cut(s) | 176, 260, 978 |
| EarI | CTCTTC | 3 cut(s) | 176, 260, 978 |
| EciI | GGCGGA | 1 cut(s) | 47 |
| Ecl136II | GAGCTC | 1 cut(s) | 202 |
| Eco130I | CCWWGG | 3 cut(s) | 82, 1602, 1836 |
| Eco147I | AGGCCT | 1 cut(s) | 1486 |
| Eco24I | GRGCYC | 3 cut(s) | 204, 445, 720 |
| Eco31I | GGTCTC | 1 cut(s) | 1019 |
| Eco32I | GATATC | 2 cut(s) | 301, 472 |
| Eco47I | GGWCC | 3 cut(s) | 653, 732, 1120 |
| Eco53kI | GAGCTC | 1 cut(s) | 202 |
| Eco57I | CTGAAG | 2 cut(s) | 908, 1776 |
| Eco81I | CCTNAGG | 2 cut(s) | 285, 1992 |
| Eco88I | CYCGRG | 2 cut(s) | 75, 719 |
| EcoICRI | GAGCTC | 1 cut(s) | 202 |
| EcoRI | GAATTC | 1 cut(s) | 206 |
| EcoRII | CCWGG | 5 cut(s) | 258, 572, 1087, 1867, 1923 |
| EcoRV | GATATC | 2 cut(s) | 301, 472 |
| EcoT14I | CCWWGG | 3 cut(s) | 82, 1602, 1836 |
| EcoT22I | ATGCAT | 3 cut(s) | 1563, 1891, 1982 |
| EcoT38I | GRGCYC | 3 cut(s) | 204, 445, 720 |
| ErhI | CCWWGG | 3 cut(s) | 82, 1602, 1836 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1809, 1841 |
| FaqI | GGGAC | 1 cut(s) | 266 |
| FauI | CCCGC | 1 cut(s) | 1966 |
| FauNDI | CATATG | 1 cut(s) | 1426 |
| FbaI | TGATCA | 2 cut(s) | 802, 1564 |
| Fnu4HI | GCNGC | 4 cut(s) | 10, 154, 1778, 1883 |
| FriOI | GRGCYC | 3 cut(s) | 204, 445, 720 |
| Fsp4HI | GCNGC | 4 cut(s) | 10, 154, 1778, 1883 |
| FspBI | CTAG | 4 cut(s) | 327, 534, 1149, 1482 |
| GlaI | GCGC | 1 cut(s) | 113 |
| GluI | GCNGC | 4 cut(s) | 10, 154, 1778, 1883 |
| GsaI | CCCAGC | 1 cut(s) | 1520 |
| GsuI | CTGGAG | 2 cut(s) | 1230, 1914 |
| HaeIII | GGCC | 6 cut(s) | 81, 105, 189, 792, 1175, 1486 |
| HapII | CCGG | 1 cut(s) | 186 |
| HgaI | GACGC | 1 cut(s) | 924 |
| HhaI | GCGC | 1 cut(s) | 114 |
| Hin6I | GCGC | 1 cut(s) | 112 |
| HinP1I | GCGC | 1 cut(s) | 112 |
| HincII | GTYRAC | 1 cut(s) | 1461 |
| HindII | GTYRAC | 1 cut(s) | 1461 |
| HinfI | GANTC | 8 cut(s) | 614, 1214, 1225, 1397, 1462, 1469, 1476, 1607 |
| HpaII | CCGG | 1 cut(s) | 186 |
| HphI | GGTGA | 7 cut(s) | 166, 175, 1181, 1617, 1652, 1663, 1727 |
| Hpy166II | GTNNAC | 5 cut(s) | 746, 1160, 1412, 1461, 1916 |
| Hpy188I | TCNGA | 4 cut(s) | 582, 757, 872, 961 |
| Hpy8I | GTNNAC | 5 cut(s) | 746, 1160, 1412, 1461, 1916 |
| Hpy99I | CGWCG | 2 cut(s) | 46, 1911 |
| HpyAV | CCTTC | 6 cut(s) | 95, 260, 365, 755, 967, 1730 |
| HpyCH4III | ACNGT | 9 cut(s) | 72, 362, 693, 708, 743, 859, 1245, 1312, 1443 |
| HpyCH4IV | ACGT | 3 cut(s) | 27, 1457, 1618 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 111, 159, 530, 928, 1298 |
| HpyF3I | CTNAG | 8 cut(s) | 285, 451, 593, 644, 1378, 1697, 1943, 1992 |
| HpySE526I | ACGT | 3 cut(s) | 27, 1457, 1618 |
| HspAI | GCGC | 1 cut(s) | 112 |
| Ksp22I | TGATCA | 2 cut(s) | 802, 1564 |
| LguI | GCTCTTC | 2 cut(s) | 176, 978 |
| LmnI | GCTCC | 3 cut(s) | 199, 268, 594 |
| Lsp1109I | GCAGC | 3 cut(s) | 140, 1789, 1894 |
| MabI | ACCWGGT | 2 cut(s) | 572, 1867 |
| MaeI | CTAG | 4 cut(s) | 327, 534, 1149, 1482 |
| MaeII | ACGT | 3 cut(s) | 27, 1457, 1618 |
| MaeIII | GTNAC | 5 cut(s) | 181, 708, 859, 1477, 1715 |
| MboII | GAAGA | 5 cut(s) | 163, 224, 247, 995, 1197 |
| MfeI | CAATTG | 1 cut(s) | 1062 |
| MflI | RGATCY | 2 cut(s) | 547, 844 |
| MhlI | GDGCHC | 3 cut(s) | 204, 445, 720 |
| MlsI | TGGCCA | 1 cut(s) | 792 |
| MluCI | AATT | 7 cut(s) | 206, 725, 765, 1062, 1199, 1372, 1963 |
| MluNI | TGGCCA | 1 cut(s) | 792 |
| MlyI | GAGTC | 4 cut(s) | 1234, 1456, 1485, 1601 |
| MmeI | TCCRAC | 4 cut(s) | 276, 761, 1098, 1414 |
| Mox20I | TGGCCA | 1 cut(s) | 792 |
| Mph1103I | ATGCAT | 3 cut(s) | 1563, 1891, 1982 |
| MroXI | GAANNNNTTC | 1 cut(s) | 955 |
| MscI | TGGCCA | 1 cut(s) | 792 |
| MseI | TTAA | 3 cut(s) | 435, 738, 1572 |
| MslI | CAYNNNNRTG | 2 cut(s) | 810, 1841 |
| Msp20I | TGGCCA | 1 cut(s) | 792 |
| MspA1I | CMGCKG | 1 cut(s) | 1292 |
| MspI | CCGG | 1 cut(s) | 186 |
| MspR9I | CCNGG | 5 cut(s) | 260, 574, 1089, 1869, 1925 |
| MunI | CAATTG | 1 cut(s) | 1062 |
| MvaI | CCWGG | 5 cut(s) | 260, 574, 1089, 1869, 1925 |
| MvnI | CGCG | 2 cut(s) | 9, 112 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 111, 159, 530, 928, 1298 |
| NcoI | CCATGG | 1 cut(s) | 1836 |
| NdeI | CATATG | 1 cut(s) | 1426 |
| NlaIV | GGNNCC | 4 cut(s) | 80, 264, 846, 1122 |
| NmuCI | GTSAC | 5 cut(s) | 181, 708, 859, 1477, 1715 |
| NsiI | ATGCAT | 3 cut(s) | 1563, 1891, 1982 |
| NspI | RCATGY | 4 cut(s) | 1061, 1278, 1889, 2022 |
| NspV | TTCGAA | 3 cut(s) | 91, 210, 949 |
| PaeI | GCATGC | 2 cut(s) | 1061, 1889 |
| PceI | AGGCCT | 1 cut(s) | 1486 |
| PciI | ACATGT | 1 cut(s) | 2018 |
| PciSI | GCTCTTC | 2 cut(s) | 176, 978 |
| PdmI | GAANNNNTTC | 1 cut(s) | 955 |
| PfeI | GAWTC | 4 cut(s) | 614, 1214, 1397, 1469 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 196, 1270 |
| PfoI | TCCNGGA | 1 cut(s) | 1087 |
| PkrI | GCNGC | 4 cut(s) | 11, 155, 1779, 1884 |
| Ple19I | CGATCG | 1 cut(s) | 110 |
| PleI | GAGTC | 4 cut(s) | 1233, 1456, 1484, 1601 |
| PpsI | GAGTC | 4 cut(s) | 1233, 1456, 1484, 1601 |
| PscI | ACATGT | 1 cut(s) | 2018 |
| Psp124BI | GAGCTC | 1 cut(s) | 204 |
| Psp1406I | AACGTT | 1 cut(s) | 1457 |
| Psp6I | CCWGG | 5 cut(s) | 258, 572, 1087, 1867, 1923 |
| PspFI | CCCAGC | 1 cut(s) | 1516 |
| PspGI | CCWGG | 5 cut(s) | 258, 572, 1087, 1867, 1923 |
| PspN4I | GGNNCC | 4 cut(s) | 80, 264, 846, 1122 |
| PspPI | GGNCC | 5 cut(s) | 79, 653, 732, 1120, 1174 |
| PsuI | RGATCY | 2 cut(s) | 547, 844 |
| PvuI | CGATCG | 1 cut(s) | 110 |
| PvuII | CAGCTG | 1 cut(s) | 1292 |
| RsaI | GTAC | 2 cut(s) | 1068, 1546 |
| RsaNI | GTAC | 2 cut(s) | 1067, 1545 |
| RseI | CAYNNNNRTG | 2 cut(s) | 810, 1841 |
| SacI | GAGCTC | 1 cut(s) | 204 |
| SapI | GCTCTTC | 2 cut(s) | 176, 978 |
| SaqAI | TTAA | 3 cut(s) | 435, 738, 1572 |
| SatI | GCNGC | 4 cut(s) | 10, 154, 1778, 1883 |
| Sau96I | GGNCC | 5 cut(s) | 79, 653, 732, 1120, 1174 |
| SchI | GAGTC | 4 cut(s) | 1234, 1456, 1485, 1601 |
| ScrFI | CCNGG | 5 cut(s) | 260, 574, 1089, 1869, 1925 |
| SduI | GDGCHC | 3 cut(s) | 204, 445, 720 |
| SexAI | ACCWGGT | 2 cut(s) | 572, 1867 |
| SfcI | CTRYAG | 1 cut(s) | 1293 |
| SfuI | TTCGAA | 3 cut(s) | 91, 210, 949 |
| SinI | GGWCC | 3 cut(s) | 653, 732, 1120 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 810, 1841 |
| SphI | GCATGC | 2 cut(s) | 1061, 1889 |
| Sse9I | AATT | 7 cut(s) | 206, 725, 765, 1062, 1199, 1372, 1963 |
| SseBI | AGGCCT | 1 cut(s) | 1486 |
| SsiI | CCGC | 9 cut(s) | 9, 32, 217, 223, 271, 524, 852, 1931, 1973 |
| SspMI | CTAG | 4 cut(s) | 327, 534, 1149, 1482 |
| SstI | GAGCTC | 1 cut(s) | 204 |
| StuI | AGGCCT | 1 cut(s) | 1486 |
| StyD4I | CCNGG | 5 cut(s) | 258, 572, 1087, 1867, 1923 |
| StyI | CCWWGG | 3 cut(s) | 82, 1602, 1836 |
| TaaI | ACNGT | 9 cut(s) | 72, 362, 693, 708, 743, 859, 1245, 1312, 1443 |
| TaiI | ACGT | 3 cut(s) | 30, 1460, 1621 |
| TaqI | TCGA | 6 cut(s) | 91, 204, 210, 949, 1240, 1906 |
| TaqII | GACCGA | 1 cut(s) | 1043 |
| TasI | AATT | 7 cut(s) | 206, 725, 765, 1062, 1199, 1372, 1963 |
| TatI | WGTACW | 1 cut(s) | 1066 |
| TauI | GCSGC | 1 cut(s) | 12 |
| TfiI | GAWTC | 4 cut(s) | 614, 1214, 1397, 1469 |
| Tru1I | TTAA | 3 cut(s) | 435, 738, 1572 |
| Tru9I | TTAA | 3 cut(s) | 435, 738, 1572 |
| TscAI | CASTG | 4 cut(s) | 344, 489, 1419, 1925 |
| TseFI | GTSAC | 5 cut(s) | 181, 708, 859, 1477, 1715 |
| TseI | GCWGC | 3 cut(s) | 153, 1777, 1882 |
| Tsp45I | GTSAC | 5 cut(s) | 181, 708, 859, 1477, 1715 |
| TspDTI | ATGAA | 7 cut(s) | 555, 774, 986, 994, 1736, 1799, 1891 |
| TspGWI | ACGGA | 3 cut(s) | 169, 568, 638 |
| TspRI | CASTG | 4 cut(s) | 344, 489, 1419, 1925 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 196, 1270 |
| VpaK11BI | GGWCC | 3 cut(s) | 653, 732, 1120 |
| XapI | RAATTY | 4 cut(s) | 206, 725, 1199, 1963 |
| XceI | RCATGY | 4 cut(s) | 1061, 1278, 1889, 2022 |
| XmnI | GAANNNNTTC | 1 cut(s) | 955 |
| XspI | CTAG | 4 cut(s) | 327, 534, 1149, 1482 |
| Zsp2I | ATGCAT | 3 cut(s) | 1563, 1891, 1982 |