Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT5G63920 |
| fragaria_vesca | FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 |
| malus_domestica | MD15G1134700.v1.1 |
| prunus_persica | Prupe.4G275500_v2.0.a1 Prupe.4G275500_v2.0.a1 |
| pyrus_communis | pycom15g12120 pycom15g12150 |
| rosa_chinensis | RchiOBHm_Chr2g0122231 |
| rosa_laevigata | RLG00000018671 |
| rosa_roxburghii | Rroxscaffold_2G00121760 |
| rosa_rugosa | Rorug02G0237200 |
| rosa_samantha | Rh2AG296000 Rh2BG304900 Rh2CG283300 Rh2DG320000 |
| rosa_wichuraiana | Rw1G008520 Rw2G023570 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 2608 |
| AccB1I | GGYRCC | 1 cut(s) | 2540 |
| AccB7I | CCANNNNNTGG | 4 cut(s) | 48, 1237, 1262, 2693 |
| AccII | CGCG | 1 cut(s) | 1577 |
| AciI | CCGC | 7 cut(s) | 69, 75, 98, 123, 1839, 2074, 2499 |
| AclI | AACGTT | 1 cut(s) | 1424 |
| AclWI | GGATC | 7 cut(s) | 88, 806, 819, 1072, 1996, 2618, 2654 |
| AcoI | YGGCCR | 2 cut(s) | 39, 757 |
| AcsI | RAATTY | 5 cut(s) | 58, 692, 1166, 1930, 2429 |
| AcuI | CTGAAG | 2 cut(s) | 1743, 2286 |
| AdeI | CACNNNGTG | 3 cut(s) | 451, 2345, 2387 |
| AfaI | GTAC | 6 cut(s) | 171, 662, 1035, 1513, 2101, 2368 |
| AflIII | ACRYGT | 1 cut(s) | 2372 |
| AgsI | TTSAA | 9 cut(s) | 359, 877, 893, 1283, 1364, 1799, 1820, 2060, 2222 |
| AjnI | CCWGG | 8 cut(s) | 110, 539, 1054, 1834, 1890, 1984, 2038, 2234 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1153, 1185 |
| AleI | CACNNNNGTG | 1 cut(s) | 2452 |
| Alw21I | GWGCWC | 4 cut(s) | 56, 1911, 2383, 2575 |
| Alw26I | GTCTC | 3 cut(s) | 986, 1252, 2365 |
| Alw44I | GTGCAC | 1 cut(s) | 2379 |
| AlwI | GGATC | 7 cut(s) | 88, 806, 819, 1072, 1996, 2618, 2654 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 2045, 2618 |
| Ama87I | CYCGRG | 2 cut(s) | 136, 904 |
| AoxI | GGCC | 8 cut(s) | 39, 113, 757, 1140, 1266, 1451, 2305, 2321 |
| ApaLI | GTGCAC | 1 cut(s) | 2379 |
| ApeKI | GCWGC | 3 cut(s) | 5, 1744, 2183 |
| ApoI | RAATTY | 5 cut(s) | 58, 692, 1166, 1930, 2429 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 922, 2170 |
| AspS9I | GGNCC | 9 cut(s) | 114, 620, 699, 1087, 1141, 1266, 2042, 2198, 2306 |
| AsuC2I | CCSGG | 1 cut(s) | 2545 |
| AsuHPI | GGTGA | 7 cut(s) | 18, 27, 1148, 1584, 1619, 1630, 2633 |
| AsuII | TTCGAA | 1 cut(s) | 62 |
| AvaI | CYCGRG | 2 cut(s) | 136, 904 |
| AvaII | GGWCC | 5 cut(s) | 620, 699, 1087, 2042, 2198 |
| BaeGI | GKGCMC | 1 cut(s) | 2383 |
| BalI | TGGCCA | 1 cut(s) | 759 |
| BamHI | GGATCC | 1 cut(s) | 811 |
| BanI | GGYRCC | 1 cut(s) | 2540 |
| BanII | GRGCYC | 2 cut(s) | 56, 412 |
| BbsI | GAAGAC | 1 cut(s) | 71 |
| Bbv12I | GWGCWC | 4 cut(s) | 56, 1911, 2383, 2575 |
| BbvI | GCAGC | 2 cut(s) | 1756, 2195 |
| BccI | CCATC | 8 cut(s) | 450, 768, 992, 1053, 1152, 1615, 1919, 2452 |
| BceAI | ACGGC | 1 cut(s) | 1382 |
| BciT130I | CCWGG | 8 cut(s) | 112, 541, 1056, 1836, 1892, 1986, 2040, 2236 |
| BciVI | GTATCC | 2 cut(s) | 870, 879 |
| BclI | TGATCA | 3 cut(s) | 769, 1531, 1846 |
| BcnI | CCSGG | 1 cut(s) | 2545 |
| BcoDI | GTCTC | 3 cut(s) | 986, 1252, 2365 |
| BfaI | CTAG | 5 cut(s) | 294, 501, 1116, 1433, 1449 |
| BfmI | CTRYAG | 1 cut(s) | 2181 |
| BfuI | GTATCC | 2 cut(s) | 870, 879 |
| BglII | AGATCT | 1 cut(s) | 514 |
| BisI | GCNGC | 4 cut(s) | 6, 1745, 2075, 2184 |
| BlsI | GCNGC | 4 cut(s) | 7, 1746, 2076, 2185 |
| Bme1390I | CCNGG | 9 cut(s) | 112, 541, 1056, 1836, 1892, 1986, 2040, 2236, 2545 |
| Bme18I | GGWCC | 5 cut(s) | 620, 699, 1087, 2042, 2198 |
| BmeT110I | CYCGRG | 2 cut(s) | 136, 904 |
| BmgT120I | GGNCC | 9 cut(s) | 114, 620, 699, 1087, 1141, 1266, 2042, 2198, 2306 |
| BmiI | GGNNCC | 6 cut(s) | 116, 813, 1089, 1267, 1982, 2542 |
| BmrFI | CCNGG | 9 cut(s) | 112, 541, 1056, 1836, 1892, 1986, 2040, 2236, 2545 |
| BmrI | ACTGGG | 2 cut(s) | 182, 673 |
| BmuI | ACTGGG | 2 cut(s) | 182, 673 |
| BpiI | GAAGAC | 1 cut(s) | 71 |
| BpmI | CTGGAG | 3 cut(s) | 1197, 1881, 2022 |
| Bpu10I | CCTNAGC | 2 cut(s) | 1910, 2574 |
| Bpu14I | TTCGAA | 1 cut(s) | 62 |
| BpuMI | CCSGG | 1 cut(s) | 2545 |
| BsaBI | GATNNNNATC | 5 cut(s) | 441, 1063, 1203, 1353, 1993 |
| BsaI | GGTCTC | 1 cut(s) | 986 |
| BsaJI | CCNNGG | 6 cut(s) | 1010, 1569, 1803, 1984, 2544, 2692 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 659, 689, 1438, 1468 |
| Bse118I | RCCGGY | 1 cut(s) | 37 |
| Bse1I | ACTGG | 7 cut(s) | 177, 304, 668, 1620, 2173, 2599, 2623 |
| Bse3DI | GCAATG | 3 cut(s) | 348, 2221, 2494 |
| Bse8I | GATNNNNATC | 5 cut(s) | 441, 1063, 1203, 1353, 1993 |
| BseBI | CCWGG | 8 cut(s) | 112, 541, 1056, 1836, 1892, 1986, 2040, 2236 |
| BseDI | CCNNGG | 6 cut(s) | 1010, 1569, 1803, 1984, 2544, 2692 |
| BseJI | GATNNNNATC | 5 cut(s) | 441, 1063, 1203, 1353, 1993 |
| BseMI | GCAATG | 3 cut(s) | 348, 2221, 2494 |
| BseMII | CTCAG | 5 cut(s) | 432, 551, 1924, 1973, 2588 |
| BseNI | ACTGG | 7 cut(s) | 177, 304, 668, 1620, 2173, 2599, 2623 |
| BseRI | GAGGAG | 2 cut(s) | 94, 299 |
| BseSI | GKGCMC | 1 cut(s) | 2383 |
| BseXI | GCAGC | 2 cut(s) | 1756, 2195 |
| BseYI | CCCAGC | 3 cut(s) | 1295, 1483, 2531 |
| BsgI | GTGCAG | 2 cut(s) | 358, 2601 |
| Bsh1236I | CGCG | 1 cut(s) | 1577 |
| BshFI | GGCC | 8 cut(s) | 41, 115, 759, 1142, 1268, 1453, 2307, 2323 |
| BshNI | GGYRCC | 1 cut(s) | 2540 |
| BsiHKAI | GWGCWC | 4 cut(s) | 56, 1911, 2383, 2575 |
| BsiHKCI | CYCGRG | 2 cut(s) | 136, 904 |
| BsiSI | CCGG | 3 cut(s) | 38, 686, 2544 |
| BslFI | GGGAC | 2 cut(s) | 118, 2614 |
| BsmAI | GTCTC | 3 cut(s) | 986, 1252, 2365 |
| BsmFI | GGGAC | 2 cut(s) | 118, 2614 |
| BsmI | GAATGC | 3 cut(s) | 1975, 2272, 2341 |
| BsnI | GGCC | 8 cut(s) | 41, 115, 759, 1142, 1268, 1453, 2307, 2323 |
| Bso31I | GGTCTC | 1 cut(s) | 986 |
| BsoBI | CYCGRG | 2 cut(s) | 136, 904 |
| Bsp119I | TTCGAA | 1 cut(s) | 62 |
| Bsp1286I | GDGCHC | 5 cut(s) | 56, 412, 1911, 2383, 2575 |
| Bsp1407I | TGTACA | 1 cut(s) | 1033 |
| Bsp19I | CCATGG | 1 cut(s) | 1803 |
| BspACI | CCGC | 7 cut(s) | 69, 75, 98, 123, 1839, 2074, 2499 |
| BspANI | GGCC | 8 cut(s) | 41, 115, 759, 1142, 1268, 1453, 2307, 2323 |
| BspCNI | CTCAG | 5 cut(s) | 431, 552, 1923, 1972, 2587 |
| BspFNI | CGCG | 1 cut(s) | 1577 |
| BspLI | GGNNCC | 6 cut(s) | 116, 813, 1089, 1267, 1982, 2542 |
| BspMAI | CTGCAG | 1 cut(s) | 2185 |
| BspPI | GGATC | 7 cut(s) | 88, 806, 819, 1072, 1996, 2618, 2654 |
| BspQI | GCTCTTC | 2 cut(s) | 28, 945 |
| BspT104I | TTCGAA | 1 cut(s) | 62 |
| BspT107I | GGYRCC | 1 cut(s) | 2540 |
| BspTNI | GGTCTC | 1 cut(s) | 986 |
| BsrDI | GCAATG | 3 cut(s) | 348, 2221, 2494 |
| BsrFI | RCCGGY | 1 cut(s) | 37 |
| BsrGI | TGTACA | 1 cut(s) | 1033 |
| BsrI | ACTGG | 7 cut(s) | 177, 304, 668, 1620, 2173, 2599, 2623 |
| BssAI | RCCGGY | 1 cut(s) | 37 |
| BssECI | CCNNGG | 6 cut(s) | 1010, 1569, 1803, 1984, 2544, 2692 |
| BssT1I | CCWWGG | 3 cut(s) | 1010, 1569, 1803 |
| Bst2UI | CCWGG | 8 cut(s) | 112, 541, 1056, 1836, 1892, 1986, 2040, 2236 |
| Bst6I | CTCTTC | 4 cut(s) | 28, 112, 945, 2497 |
| BstAPI | GCANNNNNTGC | 4 cut(s) | 497, 2031, 2256, 2588 |
| BstAUI | TGTACA | 1 cut(s) | 1033 |
| BstBI | TTCGAA | 1 cut(s) | 62 |
| BstC8I | GCNNGC | 6 cut(s) | 900, 1026, 1257, 1742, 2248, 2517 |
| BstDEI | CTNAG | 9 cut(s) | 418, 560, 611, 1345, 1664, 1910, 1959, 2141, 2574 |
| BstDSI | CCRYGG | 2 cut(s) | 1803, 2692 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 109 |
| BstFNI | CGCG | 1 cut(s) | 1577 |
| BstMAI | GTCTC | 3 cut(s) | 986, 1252, 2365 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 11, 497, 895, 1265, 2031, 2256, 2579, 2588 |
| BstNI | CCWGG | 8 cut(s) | 112, 541, 1056, 1836, 1892, 1986, 2040, 2236 |
| BstNSI | RCATGY | 4 cut(s) | 1028, 1245, 2376, 2591 |
| BstSCI | CCNGG | 9 cut(s) | 110, 539, 1054, 1834, 1890, 1984, 2038, 2234, 2543 |
| BstSFI | CTRYAG | 1 cut(s) | 2181 |
| BstSLI | GKGCMC | 1 cut(s) | 2383 |
| BstUI | CGCG | 1 cut(s) | 1577 |
| BstV1I | GCAGC | 2 cut(s) | 1756, 2195 |
| BstV2I | GAAGAC | 1 cut(s) | 71 |
| BstX2I | RGATCY | 4 cut(s) | 514, 811, 1988, 2659 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 2110 |
| BstYI | RGATCY | 4 cut(s) | 514, 811, 1988, 2659 |
| BsuI | GTATCC | 2 cut(s) | 870, 879 |
| BsuRI | GGCC | 8 cut(s) | 41, 115, 759, 1142, 1268, 1453, 2307, 2323 |
| BtgI | CCRYGG | 2 cut(s) | 1803, 2692 |
| BtgZI | GCGATG | 1 cut(s) | 1891 |
| BtsI | GCAGTG | 5 cut(s) | 9, 456, 498, 1379, 1815 |
| BtsIMutI | CAGTG | 8 cut(s) | 9, 311, 456, 498, 1379, 1613, 1815, 1967 |
| Cac8I | GCNNGC | 6 cut(s) | 900, 1026, 1257, 1742, 2248, 2517 |
| CaiI | CAGNNNCTG | 2 cut(s) | 2045, 2618 |
| Cfr10I | RCCGGY | 1 cut(s) | 37 |
| Cfr13I | GGNCC | 9 cut(s) | 114, 620, 699, 1087, 1141, 1266, 2042, 2198, 2306 |
| CseI | GACGC | 1 cut(s) | 1583 |
| CsiI | ACCWGGT | 1 cut(s) | 539 |
| Csp6I | GTAC | 6 cut(s) | 170, 661, 1034, 1512, 2100, 2367 |
| CviQI | GTAC | 6 cut(s) | 170, 661, 1034, 1512, 2100, 2367 |
| DdeI | CTNAG | 9 cut(s) | 418, 560, 611, 1345, 1664, 1910, 1959, 2141, 2574 |
| DraIII | CACNNNGTG | 3 cut(s) | 451, 2345, 2387 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 2608 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 2608 |
| EaeI | YGGCCR | 2 cut(s) | 39, 757 |
| Eam1104I | CTCTTC | 4 cut(s) | 28, 112, 945, 2497 |
| EarI | CTCTTC | 4 cut(s) | 28, 112, 945, 2497 |
| EciI | GGCGGA | 2 cut(s) | 1854, 2514 |
| Ecl136II | GAGCTC | 1 cut(s) | 54 |
| Eco130I | CCWWGG | 3 cut(s) | 1010, 1569, 1803 |
| Eco147I | AGGCCT | 2 cut(s) | 1453, 2323 |
| Eco24I | GRGCYC | 2 cut(s) | 56, 412 |
| Eco31I | GGTCTC | 1 cut(s) | 986 |
| Eco32I | GATATC | 2 cut(s) | 190, 439 |
| Eco47I | GGWCC | 5 cut(s) | 620, 699, 1087, 2042, 2198 |
| Eco53kI | GAGCTC | 1 cut(s) | 54 |
| Eco57I | CTGAAG | 2 cut(s) | 1743, 2286 |
| Eco88I | CYCGRG | 2 cut(s) | 136, 904 |
| EcoICRI | GAGCTC | 1 cut(s) | 54 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 109 |
| EcoO109I | RGGNCCY | 3 cut(s) | 114, 2042, 2306 |
| EcoRI | GAATTC | 1 cut(s) | 58 |
| EcoRII | CCWGG | 8 cut(s) | 110, 539, 1054, 1834, 1890, 1984, 2038, 2234 |
| EcoRV | GATATC | 2 cut(s) | 190, 439 |
| EcoT14I | CCWWGG | 3 cut(s) | 1010, 1569, 1803 |
| EcoT22I | ATGCAT | 1 cut(s) | 1530 |
| EcoT38I | GRGCYC | 2 cut(s) | 56, 412 |
| ErhI | CCWWGG | 3 cut(s) | 1010, 1569, 1803 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1776, 1808 |
| FaqI | GGGAC | 2 cut(s) | 118, 2614 |
| FauI | CCCGC | 1 cut(s) | 105 |
| FauNDI | CATATG | 2 cut(s) | 1069, 1393 |
| FbaI | TGATCA | 3 cut(s) | 769, 1531, 1846 |
| Fnu4HI | GCNGC | 4 cut(s) | 6, 1745, 2075, 2184 |
| FriOI | GRGCYC | 2 cut(s) | 56, 412 |
| Fsp4HI | GCNGC | 4 cut(s) | 6, 1745, 2075, 2184 |
| FspBI | CTAG | 5 cut(s) | 294, 501, 1116, 1433, 1449 |
| GluI | GCNGC | 4 cut(s) | 6, 1745, 2075, 2184 |
| GsaI | CCCAGC | 3 cut(s) | 1299, 1487, 2535 |
| GsuI | CTGGAG | 3 cut(s) | 1197, 1881, 2022 |
| HaeIII | GGCC | 8 cut(s) | 41, 115, 759, 1142, 1268, 1453, 2307, 2323 |
| HapII | CCGG | 3 cut(s) | 38, 686, 2544 |
| HgaI | GACGC | 1 cut(s) | 1583 |
| HincII | GTYRAC | 2 cut(s) | 1428, 2151 |
| HindII | GTYRAC | 2 cut(s) | 1428, 2151 |
| HinfI | GANTC | 9 cut(s) | 581, 1181, 1192, 1364, 1429, 1436, 1884, 2056, 2137 |
| HpaI | GTTAAC | 1 cut(s) | 2151 |
| HpaII | CCGG | 3 cut(s) | 38, 686, 2544 |
| HphI | GGTGA | 7 cut(s) | 18, 27, 1148, 1584, 1619, 1630, 2633 |
| Hpy166II | GTNNAC | 9 cut(s) | 159, 713, 1127, 1379, 1428, 1883, 2050, 2151, 2381 |
| Hpy188I | TCNGA | 6 cut(s) | 549, 724, 839, 917, 928, 1999 |
| Hpy188III | TCNNGA | 9 cut(s) | 461, 559, 906, 1196, 1433, 1440, 1860, 1912, 1922 |
| Hpy8I | GTNNAC | 9 cut(s) | 159, 713, 1127, 1379, 1428, 1883, 2050, 2151, 2381 |
| HpyAV | CCTTC | 5 cut(s) | 332, 722, 934, 1697, 2301 |
| HpyCH4IV | ACGT | 1 cut(s) | 1424 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 11, 497, 895, 1265, 2031, 2256, 2579, 2588 |
| HpyF3I | CTNAG | 9 cut(s) | 418, 560, 611, 1345, 1664, 1910, 1959, 2141, 2574 |
| HpySE526I | ACGT | 1 cut(s) | 1424 |
| Ksp22I | TGATCA | 3 cut(s) | 769, 1531, 1846 |
| KspAI | GTTAAC | 1 cut(s) | 2151 |
| LguI | GCTCTTC | 2 cut(s) | 28, 945 |
| LmnI | GCTCC | 5 cut(s) | 51, 176, 561, 1678, 1986 |
| Lsp1109I | GCAGC | 2 cut(s) | 1756, 2195 |
| MabI | ACCWGGT | 1 cut(s) | 539 |
| MaeI | CTAG | 5 cut(s) | 294, 501, 1116, 1433, 1449 |
| MaeII | ACGT | 1 cut(s) | 1424 |
| MaeIII | GTNAC | 6 cut(s) | 33, 675, 826, 1444, 1682, 2664 |
| MboII | GAAGA | 9 cut(s) | 15, 76, 99, 962, 2268, 2292, 2436, 2486, 2514 |
| MfeI | CAATTG | 1 cut(s) | 1029 |
| MflI | RGATCY | 4 cut(s) | 514, 811, 1988, 2659 |
| MhlI | GDGCHC | 5 cut(s) | 56, 412, 1911, 2383, 2575 |
| MlsI | TGGCCA | 1 cut(s) | 759 |
| MluNI | TGGCCA | 1 cut(s) | 759 |
| MlyI | GAGTC | 3 cut(s) | 1201, 1423, 1878 |
| MmeI | TCCRAC | 6 cut(s) | 199, 223, 243, 728, 1065, 1381 |
| Mox20I | TGGCCA | 1 cut(s) | 759 |
| Mph1103I | ATGCAT | 1 cut(s) | 1530 |
| MroXI | GAANNNNTTC | 2 cut(s) | 922, 2170 |
| MscI | TGGCCA | 1 cut(s) | 759 |
| MseI | TTAA | 6 cut(s) | 270, 402, 705, 1466, 1539, 2150 |
| MslI | CAYNNNNRTG | 3 cut(s) | 777, 1808, 2452 |
| Msp20I | TGGCCA | 1 cut(s) | 759 |
| MspA1I | CMGCKG | 1 cut(s) | 1259 |
| MspI | CCGG | 3 cut(s) | 38, 686, 2544 |
| MspR9I | CCNGG | 9 cut(s) | 112, 541, 1056, 1836, 1892, 1986, 2040, 2236, 2545 |
| MunI | CAATTG | 1 cut(s) | 1029 |
| Mva1269I | GAATGC | 3 cut(s) | 1975, 2272, 2341 |
| MvaI | CCWGG | 8 cut(s) | 112, 541, 1056, 1836, 1892, 1986, 2040, 2236 |
| MvnI | CGCG | 1 cut(s) | 1577 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 11, 497, 895, 1265, 2031, 2256, 2579, 2588 |
| NciI | CCSGG | 1 cut(s) | 2545 |
| NcoI | CCATGG | 1 cut(s) | 1803 |
| NdeI | CATATG | 2 cut(s) | 1069, 1393 |
| NlaIV | GGNNCC | 6 cut(s) | 116, 813, 1089, 1267, 1982, 2542 |
| NmuCI | GTSAC | 5 cut(s) | 33, 675, 826, 1682, 2664 |
| NsiI | ATGCAT | 1 cut(s) | 1530 |
| NspI | RCATGY | 4 cut(s) | 1028, 1245, 2376, 2591 |
| NspV | TTCGAA | 1 cut(s) | 62 |
| OliI | CACNNNNGTG | 1 cut(s) | 2452 |
| PaeI | GCATGC | 1 cut(s) | 1028 |
| PaeR7I | CTCGAG | 1 cut(s) | 904 |
| PceI | AGGCCT | 2 cut(s) | 1453, 2323 |
| PciI | ACATGT | 1 cut(s) | 2372 |
| PciSI | GCTCTTC | 2 cut(s) | 28, 945 |
| PctI | GAATGC | 3 cut(s) | 1975, 2272, 2341 |
| PdmI | GAANNNNTTC | 2 cut(s) | 922, 2170 |
| PfeI | GAWTC | 6 cut(s) | 581, 1181, 1364, 1436, 2056, 2137 |
| PflMI | CCANNNNNTGG | 4 cut(s) | 48, 1237, 1262, 2693 |
| PfoI | TCCNGGA | 1 cut(s) | 1054 |
| PkrI | GCNGC | 4 cut(s) | 7, 1746, 2076, 2185 |
| PleI | GAGTC | 3 cut(s) | 1200, 1423, 1878 |
| PpsI | GAGTC | 3 cut(s) | 1200, 1423, 1878 |
| PpuMI | RGGWCCY | 1 cut(s) | 2042 |
| PscI | ACATGT | 1 cut(s) | 2372 |
| Psp124BI | GAGCTC | 1 cut(s) | 56 |
| Psp1406I | AACGTT | 1 cut(s) | 1424 |
| Psp5II | RGGWCCY | 1 cut(s) | 2042 |
| Psp6I | CCWGG | 8 cut(s) | 110, 539, 1054, 1834, 1890, 1984, 2038, 2234 |
| PspFI | CCCAGC | 3 cut(s) | 1295, 1483, 2531 |
| PspGI | CCWGG | 8 cut(s) | 110, 539, 1054, 1834, 1890, 1984, 2038, 2234 |
| PspN4I | GGNNCC | 6 cut(s) | 116, 813, 1089, 1267, 1982, 2542 |
| PspPI | GGNCC | 9 cut(s) | 114, 620, 699, 1087, 1141, 1266, 2042, 2198, 2306 |
| PspPPI | RGGWCCY | 1 cut(s) | 2042 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 2389, 2421 |
| PstI | CTGCAG | 1 cut(s) | 2185 |
| PstNI | CAGNNNCTG | 2 cut(s) | 2045, 2618 |
| PsuI | RGATCY | 4 cut(s) | 514, 811, 1988, 2659 |
| PvuII | CAGCTG | 1 cut(s) | 1259 |
| RsaI | GTAC | 6 cut(s) | 171, 662, 1035, 1513, 2101, 2368 |
| RsaNI | GTAC | 6 cut(s) | 170, 661, 1034, 1512, 2100, 2367 |
| RseI | CAYNNNNRTG | 3 cut(s) | 777, 1808, 2452 |
| SacI | GAGCTC | 1 cut(s) | 56 |
| SapI | GCTCTTC | 2 cut(s) | 28, 945 |
| SaqAI | TTAA | 6 cut(s) | 270, 402, 705, 1466, 1539, 2150 |
| SatI | GCNGC | 4 cut(s) | 6, 1745, 2075, 2184 |
| Sau96I | GGNCC | 9 cut(s) | 114, 620, 699, 1087, 1141, 1266, 2042, 2198, 2306 |
| SchI | GAGTC | 3 cut(s) | 1201, 1423, 1878 |
| ScrFI | CCNGG | 9 cut(s) | 112, 541, 1056, 1836, 1892, 1986, 2040, 2236, 2545 |
| SduI | GDGCHC | 5 cut(s) | 56, 412, 1911, 2383, 2575 |
| SexAI | ACCWGGT | 1 cut(s) | 539 |
| SfcI | CTRYAG | 1 cut(s) | 2181 |
| Sfr274I | CTCGAG | 1 cut(s) | 904 |
| SfuI | TTCGAA | 1 cut(s) | 62 |
| SinI | GGWCC | 5 cut(s) | 620, 699, 1087, 2042, 2198 |
| SlaI | CTCGAG | 1 cut(s) | 904 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 777, 1808, 2452 |
| SmlI | CTYRAG | 1 cut(s) | 904 |
| SmoI | CTYRAG | 1 cut(s) | 904 |
| SphI | GCATGC | 1 cut(s) | 1028 |
| SseBI | AGGCCT | 2 cut(s) | 1453, 2323 |
| SsiI | CCGC | 7 cut(s) | 69, 75, 98, 123, 1839, 2074, 2499 |
| SspI | AATATT | 1 cut(s) | 2020 |
| SspMI | CTAG | 5 cut(s) | 294, 501, 1116, 1433, 1449 |
| SstI | GAGCTC | 1 cut(s) | 56 |
| StuI | AGGCCT | 2 cut(s) | 1453, 2323 |
| StyD4I | CCNGG | 9 cut(s) | 110, 539, 1054, 1834, 1890, 1984, 2038, 2234, 2543 |
| StyI | CCWWGG | 3 cut(s) | 1010, 1569, 1803 |
| TaiI | ACGT | 1 cut(s) | 1427 |
| TaqI | TCGA | 8 cut(s) | 56, 62, 905, 985, 1184, 1207, 2311, 2326 |
| TaqII | GACCGA | 2 cut(s) | 1010, 2166 |
| TatI | WGTACW | 2 cut(s) | 1033, 2366 |
| TauI | GCSGC | 1 cut(s) | 2077 |
| TfiI | GAWTC | 6 cut(s) | 581, 1181, 1364, 1436, 2056, 2137 |
| Tru1I | TTAA | 6 cut(s) | 270, 402, 705, 1466, 1539, 2150 |
| Tru9I | TTAA | 6 cut(s) | 270, 402, 705, 1466, 1539, 2150 |
| TscAI | CASTG | 8 cut(s) | 16, 311, 456, 498, 1386, 1620, 1815, 1967 |
| TseFI | GTSAC | 5 cut(s) | 33, 675, 826, 1682, 2664 |
| TseI | GCWGC | 3 cut(s) | 5, 1744, 2183 |
| Tsp45I | GTSAC | 5 cut(s) | 33, 675, 826, 1682, 2664 |
| TspDTI | ATGAA | 9 cut(s) | 522, 741, 810, 953, 1703, 1766, 1858, 2019, 2348 |
| TspGWI | ACGGA | 5 cut(s) | 21, 605, 855, 2168, 2652 |
| TspRI | CASTG | 8 cut(s) | 16, 311, 456, 498, 1386, 1620, 1815, 1967 |
| Van91I | CCANNNNNTGG | 4 cut(s) | 48, 1237, 1262, 2693 |
| VneI | GTGCAC | 1 cut(s) | 2379 |
| VpaK11BI | GGWCC | 5 cut(s) | 620, 699, 1087, 2042, 2198 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 109 |
| XapI | RAATTY | 5 cut(s) | 58, 692, 1166, 1930, 2429 |
| XbaI | TCTAGA | 1 cut(s) | 1432 |
| XceI | RCATGY | 4 cut(s) | 1028, 1245, 2376, 2591 |
| XhoI | CTCGAG | 1 cut(s) | 904 |
| XmnI | GAANNNNTTC | 2 cut(s) | 922, 2170 |
| XspI | CTAG | 5 cut(s) | 294, 501, 1116, 1433, 1449 |
| Zsp2I | ATGCAT | 1 cut(s) | 1530 |