Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0006666

Physical Location & Seq

Genomic Coordinates Forward (+)

8259139 .. 8262810

Gene Structure 3672 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom15g12120.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1260 bp

ATGTCAGGGAGTGGGGGTAGTATTAGGGTGCTGAACGTGGCGGAGAAGCCGTCGGTGGCCAAGTCGGTGGCGACATTACTGTCAGGGAACCAAGGGCAAGGGCTGAGAGTGAGGGAAGGCCGATCCCGCTACAACAAAATCTTCGAGTTCAACTACTCCATCAATGGCCGCCCCTGCCACATGCTCGTCACCTCCGTCACCGGCCACCTCATGGAGCTCGAATTTGAGGACCGCTTTCGAAAATGGCACGCCTGCGACCCCGCCCTTCTCTTTACCGCTCCCGTCCATAAAAAAGTCCCTGAGGACAAGTTGGATATCAAGAGGACGTTAGAGGAGGAAGCAAGGAGGTGCCAGTGGCTTGTCTTGTGGCTCGATTGTGATAGAGAAGGAGAAAACATTGCGTTTGAAGTCATAGAAGTATGCACAGCCGTGAATCGTCATCTTACCATAAGGAGGGCTCGTTTCTCAGCTTTGACTGAAAGGGATATCTATAATGCGGTGCAGAATCTTGTCAACGCAAACAAATTCTTTGCTGATGCAGTGGATGCTAGGCAAGAAATAGATCTTCGGATTGGTGCTTCGTTTACTAGGTTTCAGACTATGCTGCTGAGAGATGCATTTGTTATTGATTCCACCGCAAGTGACAGAAATCTTGTTCTAAGTTATGGTCCTTGCCAGTTCCCTACACTTGGATTTGTTGTGGAGAGATATTGGGAAATACAGTTGCACGAGCCAGAGGAGTTTTGGACCATAAACTGTTCACACAAATCAGATGAAGGCACTGCTACATTCCATTGGATGCGTGGGCATTTATTCGACTATGCTTGTGCTGTTGTTATTTATGAAATGTGTGTTGAGGAACCTACTGCTACTGTGACAAAGGTTAGACAACAGGAGAAGCTGAAAAAGCCTCCAAATCCTCTGAATACAATTCAGCTTGAACAGCGTGCCTCAATATACTTTCGGATGAGTTCTGAACACACAATGAAGGTGGCAGAAGAGCTGTACCAAGCTGGTTTCATCAGTTATCCTCGTACGGAGACTGATGGCTTCTCTGAAAGGACGGACTTACATGTGATTGTGGAAGAACAACAAAGGCATCCTGGATGGGGATCATATGCTCAACGATTGTTGGACCCAGCATCTGGGCTTTGGAAAAACCCTAGCAGTGGTGGACATGATGACAAGGCCCATCCACCTATCCACCCCACTAAATTTTCTTCTGGAGAATCAGGATGGAGTCAAGATCGAACCATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000278 GO:0000280 GO:0000712 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0003916 GO:0003917 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006260 GO:0006265 GO:0006281 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007127 GO:0007131 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0016043 GO:0016604 GO:0016605 GO:0016853 GO:0022402 GO:0022414 GO:0031974 GO:0031981 GO:0033554 GO:0034641 GO:0034645 GO:0035825 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045132 GO:0046483 GO:0048285 GO:0050896 GO:0051276 GO:0051304 GO:0051307 GO:0051321 GO:0051716 GO:0061982 GO:0070013 GO:0071103 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:0098813 GO:0140013 GO:0140097 GO:1901360 GO:1901363 GO:1901576 GO:1903046

KEGG Pathways

Metabolic & Signaling

ko03440 ko03460 map03440 map03460

Pfam Domains

Protein Families

Topoisom_bac Toprim zf-C4_Topoisom zf-CCHC zf-GRF

Protein Analysis

420

Amino Acids

47.98

Weight (kDa)

6.22

Isoelectric Point (pI)

46.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Toprim	PF01751	11 - 157	1.6e-13	Toprim domain
Topoisom_bac	PF01131	177 - 406	1.2e-54	DNA topoisomerase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006666)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G63920
fragaria_vesca	FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710 FvH4_1g24710
malus_domestica	MD15G1134700.v1.1
prunus_persica	Prupe.4G275500_v2.0.a1 Prupe.4G275500_v2.0.a1
pyrus_communis	pycom15g12120 pycom15g12150
rosa_chinensis	RchiOBHm_Chr2g0122231
rosa_laevigata	RLG00000018671
rosa_roxburghii	Rroxscaffold_2G00121760
rosa_rugosa	Rorug02G0237200
rosa_samantha	Rh2AG296000 Rh2BG304900 Rh2CG283300 Rh2DG320000
rosa_wichuraiana	Rw1G008520 Rw2G023570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	79
AccB1I	GGYRCC	1 cut(s)	348
AccB7I	CCANNNNNTGG	2 cut(s)	211, 1145
AccBSI	CCGCTC	1 cut(s)	278
AciI	CCGC	8 cut(s)	41, 127, 169, 232, 261, 276, 497, 636
AclWI	GGATC	2 cut(s)	117, 1120
AcoI	YGGCCR	3 cut(s)	57, 166, 202
AcsI	RAATTY	3 cut(s)	221, 524, 1214
AfaI	GTAC	2 cut(s)	1007, 1036
AfiI	CCNNNNNNNGG	6 cut(s)	211, 453, 689, 1109, 1145, 1169
AflIII	ACRYGT	1 cut(s)	1072
AgsI	TTSAA	3 cut(s)	151, 407, 941
AjnI	CCWGG	1 cut(s)	1102
AleI	CACNNNNGTG	1 cut(s)	428
AluBI	AGCT	6 cut(s)	217, 470, 901, 937, 1003, 1013
AluI	AGCT	6 cut(s)	217, 470, 901, 937, 1003, 1013
Alw21I	GWGCWC	1 cut(s)	219
Alw26I	GTCTC	1 cut(s)	1034
AlwI	GGATC	2 cut(s)	117, 1120
AlwNI	CAGNNNCTG	1 cut(s)	1145
AoxI	GGCC	5 cut(s)	57, 118, 166, 202, 1188
ApeKI	GCWGC	1 cut(s)	604
ApoI	RAATTY	3 cut(s)	221, 524, 1214
AspS9I	GGNCC	5 cut(s)	229, 668, 747, 1135, 1189
AsuHPI	GGTGA	2 cut(s)	181, 190
AsuII	TTCGAA	1 cut(s)	238
AvaII	GGWCC	4 cut(s)	229, 668, 747, 1135
AxyI	CCTNAGG	1 cut(s)	300
BalI	TGGCCA	1 cut(s)	59
BanI	GGYRCC	1 cut(s)	348
BanII	GRGCYC	2 cut(s)	219, 460
BauI	CACGAG	1 cut(s)	728
Bbv12I	GWGCWC	1 cut(s)	219
BbvI	GCAGC	1 cut(s)	591
BccI	CCATC	5 cut(s)	167, 1040, 1101, 1200, 1230
BceAI	ACGGC	2 cut(s)	34, 413
BciT130I	CCWGG	1 cut(s)	1104
BcoDI	GTCTC	1 cut(s)	1034
BfaI	CTAG	3 cut(s)	549, 588, 1164
BglII	AGATCT	1 cut(s)	562
BisI	GCNGC	2 cut(s)	169, 605
BlsI	GCNGC	2 cut(s)	170, 606
Bme1390I	CCNGG	1 cut(s)	1104
Bme18I	GGWCC	4 cut(s)	229, 668, 747, 1135
BmgT120I	GGNCC	5 cut(s)	229, 668, 747, 1135, 1189
BmiI	GGNNCC	4 cut(s)	89, 350, 861, 1137
BmrFI	CCNGG	1 cut(s)	1104
BmsI	GCATC	6 cut(s)	526, 535, 604, 789, 1108, 1151
BpmI	CTGGAG	1 cut(s)	1245
Bpu14I	TTCGAA	1 cut(s)	238
BsaBI	GATNNNNATC	1 cut(s)	1111
BsaJI	CCNNGG	1 cut(s)	91
BsaXI	ACNNNNNCTCC	5 cut(s)	31, 35, 65, 445, 475
Bsc4I	CCNNNNNNNGG	6 cut(s)	211, 453, 689, 1109, 1145, 1169
Bse118I	RCCGGY	1 cut(s)	200
Bse1I	ACTGG	2 cut(s)	352, 676
Bse21I	CCTNAGG	1 cut(s)	300
Bse3DI	GCAATG	1 cut(s)	396
Bse8I	GATNNNNATC	1 cut(s)	1111
BseBI	CCWGG	1 cut(s)	1104
BseDI	CCNNGG	1 cut(s)	91
BseGI	GGATG	7 cut(s)	550, 804, 972, 1099, 1112, 1192, 1241
BseJI	GATNNNNATC	1 cut(s)	1111
BseLI	CCNNNNNNNGG	6 cut(s)	211, 453, 689, 1109, 1145, 1169
BseMI	GCAATG	1 cut(s)	396
BseMII	CTCAG	4 cut(s)	95, 291, 480, 599
BseNI	ACTGG	2 cut(s)	352, 676
BseRI	GAGGAG	2 cut(s)	347, 752
BseXI	GCAGC	1 cut(s)	591
BseYI	CCCAGC	1 cut(s)	1138
BsgI	GTGCAG	1 cut(s)	521
BshFI	GGCC	5 cut(s)	59, 120, 168, 204, 1190
BshNI	GGYRCC	1 cut(s)	348
BsiHKAI	GWGCWC	1 cut(s)	219
BsiSI	CCGG	1 cut(s)	201
BsiWI	CGTACG	1 cut(s)	1034
BslFI	GGGAC	1 cut(s)	281
BslI	CCNNNNNNNGG	6 cut(s)	211, 453, 689, 1109, 1145, 1169
BsmAI	GTCTC	1 cut(s)	1034
BsmFI	GGGAC	1 cut(s)	281
BsnI	GGCC	5 cut(s)	59, 120, 168, 204, 1190
Bsp119I	TTCGAA	1 cut(s)	238
Bsp1286I	GDGCHC	2 cut(s)	219, 460
Bsp143I	GATC	4 cut(s)	122, 562, 1112, 1246
BspACI	CCGC	8 cut(s)	41, 127, 169, 232, 261, 276, 497, 636
BspANI	GGCC	5 cut(s)	59, 120, 168, 204, 1190
BspCNI	CTCAG	4 cut(s)	96, 292, 479, 600
BspLI	GGNNCC	4 cut(s)	89, 350, 861, 1137
BspPI	GGATC	2 cut(s)	117, 1120
BspQI	GCTCTTC	1 cut(s)	993
BspT104I	TTCGAA	1 cut(s)	238
BspT107I	GGYRCC	1 cut(s)	348
BsrBI	CCGCTC	1 cut(s)	278
BsrDI	GCAATG	1 cut(s)	396
BsrFI	RCCGGY	1 cut(s)	200
BsrI	ACTGG	2 cut(s)	352, 676
BssAI	RCCGGY	1 cut(s)	200
BssECI	CCNNGG	1 cut(s)	91
BssMI	GATC	4 cut(s)	122, 562, 1112, 1246
BssSI	CACGAG	1 cut(s)	728
BssT1I	CCWWGG	1 cut(s)	91
Bst2BI	CACGAG	1 cut(s)	728
Bst2UI	CCWGG	1 cut(s)	1104
Bst4CI	ACNGT	4 cut(s)	81, 723, 758, 874
Bst6I	CTCTTC	1 cut(s)	993
BstAPI	GCANNNNNTGC	1 cut(s)	545
BstBI	TTCGAA	1 cut(s)	238
BstC8I	GCNNGC	3 cut(s)	249, 253, 948
BstDEI	CTNAG	5 cut(s)	104, 300, 466, 608, 659
BstF5I	GGATG	7 cut(s)	550, 804, 972, 1099, 1112, 1192, 1241
BstKTI	GATC	4 cut(s)	125, 565, 1115, 1249
BstMAI	GTCTC	1 cut(s)	1034
BstMBI	GATC	4 cut(s)	122, 562, 1112, 1246
BstMWI	GCNNNNNNNGC	5 cut(s)	126, 174, 545, 907, 943
BstNI	CCWGG	1 cut(s)	1104
BstNSI	RCATGY	2 cut(s)	184, 1076
BstSCI	CCNGG	1 cut(s)	1102
BstV1I	GCAGC	1 cut(s)	591
BstX2I	RGATCY	1 cut(s)	562
BstXI	CCANNNNNNTGG	1 cut(s)	67
BstYI	RGATCY	1 cut(s)	562
Bsu36I	CCTNAGG	1 cut(s)	300
BsuRI	GGCC	5 cut(s)	59, 120, 168, 204, 1190
BtsCI	GGATG	7 cut(s)	550, 804, 972, 1099, 1112, 1192, 1241
BtsI	GCAGTG	3 cut(s)	546, 780, 1174
BtsIMutI	CAGTG	4 cut(s)	359, 546, 780, 1174
Cac8I	GCNNGC	3 cut(s)	249, 253, 948
CaiI	CAGNNNCTG	1 cut(s)	1145
Cfr10I	RCCGGY	1 cut(s)	200
Cfr13I	GGNCC	5 cut(s)	229, 668, 747, 1135, 1189
Csp6I	GTAC	2 cut(s)	1006, 1035
CspCI	CAANNNNNGTGG	2 cut(s)	972, 1007
CviAII	CATG	5 cut(s)	181, 211, 1073, 1178, 1255
CviQI	GTAC	2 cut(s)	1006, 1035
DdeI	CTNAG	5 cut(s)	104, 300, 466, 608, 659
DpnI	GATC	4 cut(s)	124, 564, 1114, 1248
DpnII	GATC	4 cut(s)	122, 562, 1112, 1246
DrdI	GACNNNNNNGTC	1 cut(s)	79
DseDI	GACNNNNNNGTC	1 cut(s)	79
EaeI	YGGCCR	3 cut(s)	57, 166, 202
Eam1104I	CTCTTC	1 cut(s)	993
EarI	CTCTTC	1 cut(s)	993
EciI	GGCGGA	1 cut(s)	56
Ecl136II	GAGCTC	1 cut(s)	217
Eco130I	CCWWGG	1 cut(s)	91
Eco24I	GRGCYC	2 cut(s)	219, 460
Eco32I	GATATC	2 cut(s)	316, 487
Eco47I	GGWCC	4 cut(s)	229, 668, 747, 1135
Eco53kI	GAGCTC	1 cut(s)	217
Eco81I	CCTNAGG	1 cut(s)	300
EcoICRI	GAGCTC	1 cut(s)	217
EcoRII	CCWGG	1 cut(s)	1102
EcoRV	GATATC	2 cut(s)	316, 487
EcoT14I	CCWWGG	1 cut(s)	91
EcoT22I	ATGCAT	1 cut(s)	619
EcoT38I	GRGCYC	2 cut(s)	219, 460
ErhI	CCWWGG	1 cut(s)	91
FaeI	CATG	5 cut(s)	184, 214, 1076, 1181, 1258
FaqI	GGGAC	1 cut(s)	281
FatI	CATG	5 cut(s)	180, 210, 1072, 1177, 1254
FauI	CCCGC	2 cut(s)	134, 268
FauNDI	CATATG	1 cut(s)	1117
Fnu4HI	GCNGC	2 cut(s)	169, 605
FokI	GGATG	7 cut(s)	557, 811, 979, 1086, 1119, 1179, 1248
FriOI	GRGCYC	2 cut(s)	219, 460
Fsp4HI	GCNGC	2 cut(s)	169, 605
FspBI	CTAG	3 cut(s)	549, 588, 1164
GluI	GCNGC	2 cut(s)	169, 605
GsaI	CCCAGC	1 cut(s)	1142
GsuI	CTGGAG	1 cut(s)	1245
HaeIII	GGCC	5 cut(s)	59, 120, 168, 204, 1190
HapII	CCGG	1 cut(s)	201
Hin1II	CATG	5 cut(s)	184, 214, 1076, 1181, 1258
HincII	GTYRAC	1 cut(s)	514
HindII	GTYRAC	1 cut(s)	514
HinfI	GANTC	5 cut(s)	433, 505, 629, 1229, 1240
HpaII	CCGG	1 cut(s)	201
HphI	GGTGA	2 cut(s)	181, 190
Hpy166II	GTNNAC	4 cut(s)	514, 585, 761, 1175
Hpy188I	TCNGA	7 cut(s)	570, 597, 772, 924, 966, 976, 1057
Hpy188III	TCNNGA	4 cut(s)	319, 1224, 1233, 1244
Hpy8I	GTNNAC	4 cut(s)	514, 585, 761, 1175
Hpy99I	CGWCG	1 cut(s)	55
HpyAV	CCTTC	5 cut(s)	110, 275, 380, 770, 982
HpyCH4III	ACNGT	4 cut(s)	81, 723, 758, 874
HpyCH4IV	ACGT	2 cut(s)	36, 326
HpyCH4V	TGCA	5 cut(s)	423, 502, 539, 617, 727
HpyF10VI	GCNNNNNNNGC	5 cut(s)	126, 174, 545, 907, 943
HpyF3I	CTNAG	5 cut(s)	104, 300, 466, 608, 659
HpySE526I	ACGT	2 cut(s)	36, 326
Hsp92II	CATG	5 cut(s)	184, 214, 1076, 1181, 1258
Kzo9I	GATC	4 cut(s)	122, 562, 1112, 1246
LguI	GCTCTTC	1 cut(s)	993
LmnI	GCTCC	2 cut(s)	214, 283
Lsp1109I	GCAGC	1 cut(s)	591
LweI	GCATC	6 cut(s)	526, 535, 604, 789, 1108, 1151
MaeI	CTAG	3 cut(s)	549, 588, 1164
MaeII	ACGT	2 cut(s)	36, 326
MaeIII	GTNAC	4 cut(s)	187, 196, 641, 874
MalI	GATC	4 cut(s)	124, 564, 1114, 1248
MbiI	CCGCTC	1 cut(s)	278
MboI	GATC	4 cut(s)	122, 562, 1112, 1246
MboII	GAAGA	5 cut(s)	133, 557, 1010, 1097, 1212
MflI	RGATCY	1 cut(s)	562
MhlI	GDGCHC	2 cut(s)	219, 460
MlsI	TGGCCA	1 cut(s)	59
MluCI	AATT	4 cut(s)	221, 524, 930, 1214
MluNI	TGGCCA	1 cut(s)	59
MlyI	GAGTC	1 cut(s)	1249
MmeI	TCCRAC	2 cut(s)	291, 1113
Mox20I	TGGCCA	1 cut(s)	59
Mph1103I	ATGCAT	1 cut(s)	619
MscI	TGGCCA	1 cut(s)	59
MslI	CAYNNNNRTG	1 cut(s)	428
Msp20I	TGGCCA	1 cut(s)	59
MspI	CCGG	1 cut(s)	201
MspR9I	CCNGG	1 cut(s)	1104
MvaI	CCWGG	1 cut(s)	1104
MwoI	GCNNNNNNNGC	5 cut(s)	126, 174, 545, 907, 943
NdeI	CATATG	1 cut(s)	1117
NdeII	GATC	4 cut(s)	122, 562, 1112, 1246
NlaIII	CATG	5 cut(s)	184, 214, 1076, 1181, 1258
NlaIV	GGNNCC	4 cut(s)	89, 350, 861, 1137
NmuCI	GTSAC	4 cut(s)	187, 196, 641, 874
NsiI	ATGCAT	1 cut(s)	619
NspI	RCATGY	2 cut(s)	184, 1076
NspV	TTCGAA	1 cut(s)	238
OliI	CACNNNNGTG	1 cut(s)	428
PciI	ACATGT	1 cut(s)	1072
PciSI	GCTCTTC	1 cut(s)	993
PcsI	WCGNNNNNNNCGW	1 cut(s)	192
PfeI	GAWTC	4 cut(s)	433, 505, 629, 1229
Pfl23II	CGTACG	1 cut(s)	1034
PflMI	CCANNNNNTGG	2 cut(s)	211, 1145
PfoI	TCCNGGA	1 cut(s)	1102
PkrI	GCNGC	2 cut(s)	170, 606
PleI	GAGTC	1 cut(s)	1248
PpsI	GAGTC	1 cut(s)	1248
PscI	ACATGT	1 cut(s)	1072
Psp124BI	GAGCTC	1 cut(s)	219
Psp6I	CCWGG	1 cut(s)	1102
PspFI	CCCAGC	1 cut(s)	1138
PspGI	CCWGG	1 cut(s)	1102
PspLI	CGTACG	1 cut(s)	1034
PspN4I	GGNNCC	4 cut(s)	89, 350, 861, 1137
PspPI	GGNCC	5 cut(s)	229, 668, 747, 1135, 1189
PstNI	CAGNNNCTG	1 cut(s)	1145
PsuI	RGATCY	1 cut(s)	562
RsaI	GTAC	2 cut(s)	1007, 1036
RsaNI	GTAC	2 cut(s)	1006, 1035
RseI	CAYNNNNRTG	1 cut(s)	428
SacI	GAGCTC	1 cut(s)	219
SapI	GCTCTTC	1 cut(s)	993
SatI	GCNGC	2 cut(s)	169, 605
Sau3AI	GATC	4 cut(s)	122, 562, 1112, 1246
Sau96I	GGNCC	5 cut(s)	229, 668, 747, 1135, 1189
SchI	GAGTC	1 cut(s)	1249
ScrFI	CCNGG	1 cut(s)	1104
SduI	GDGCHC	2 cut(s)	219, 460
SfaNI	GCATC	6 cut(s)	526, 535, 604, 789, 1108, 1151
SfuI	TTCGAA	1 cut(s)	238
SinI	GGWCC	4 cut(s)	229, 668, 747, 1135
SmiMI	CAYNNNNRTG	1 cut(s)	428
Sse9I	AATT	4 cut(s)	221, 524, 930, 1214
SsiI	CCGC	8 cut(s)	41, 127, 169, 232, 261, 276, 497, 636
SspMI	CTAG	3 cut(s)	549, 588, 1164
SstI	GAGCTC	1 cut(s)	219
StyD4I	CCNGG	1 cut(s)	1102
StyI	CCWWGG	1 cut(s)	91
TaaI	ACNGT	4 cut(s)	81, 723, 758, 874
TaiI	ACGT	2 cut(s)	39, 329
TaqI	TCGA	6 cut(s)	144, 219, 238, 372, 816, 1249
TasI	AATT	4 cut(s)	221, 524, 930, 1214
TauI	GCSGC	1 cut(s)	171
TfiI	GAWTC	4 cut(s)	433, 505, 629, 1229
TscAI	CASTG	4 cut(s)	359, 546, 787, 1174
TseFI	GTSAC	4 cut(s)	187, 196, 641, 874
TseI	GCWGC	1 cut(s)	604
Tsp45I	GTSAC	4 cut(s)	187, 196, 641, 874
TspDTI	ATGAA	4 cut(s)	789, 858, 1001, 1009
TspGWI	ACGGA	3 cut(s)	184, 1052, 1079
TspRI	CASTG	4 cut(s)	359, 546, 787, 1174
Van91I	CCANNNNNTGG	2 cut(s)	211, 1145
VpaK11BI	GGWCC	4 cut(s)	229, 668, 747, 1135
XapI	RAATTY	3 cut(s)	221, 524, 1214
XceI	RCATGY	2 cut(s)	184, 1076
XcmI	CCANNNNNNNNNTGG	2 cut(s)	741, 800
XspI	CTAG	3 cut(s)	549, 588, 1164
Zsp2I	ATGCAT	1 cut(s)	619

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.