AT5G64360

DNAJ heat shock N-terminal domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009201
Physical Location & Seq
Reverse (-)
25736171 .. 25738676
2506 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G64360.5

Sequence Viewer

Length: 1269 bp
ATGGTAGGCGGACGCGGCGGAGTTGGCGGTGGTGGTGGTAACAGAGTGGAGGCGGATCAGTGGCTTGTAACATCGGAGAAGCTTCTAGCGTCTAGTGATCTTCAAGGAGCAAAGACATTTGCGATCCGCGCATGCGAAGCTGACCCGACCCGAGCAGAAGCTGCAGACTACATCCTCGCAATCTGCGACATTCTTCTCGCCGGAGAGACTCGTTTAGGTGATTCCAACTTGCCAGACTGGTACGCCGTTCTCCGACTCGGTCGTTTAGCTCAAAACCCTGAACACGTCGCGACTCAGTACCGTAGACTCGCTCTTCTCCTCAATCCTTCCGTTAATCGTCTCCCATTCGCCGATCGAGCTCTTAAAATCGTCTCCGATGCTTGGTTCGTTCTCTCTGATCCGTTCAAGAAATCATTCTACGACCGAGAATTGCAACTTAGTCAACTCGGTCAATCTGGATTCCATCCTCAAACCCAATCGCATCAAAATTTCCAGTGGGAACCTAGCAGTAGCACCGCCGTATATCCGCCACCGCGGTCTCAGAGCCAGGCTGGGACCTCGGCGGATCCTATGGCGACGAGCTTCTGGACCGCTTGCCCTTACTGCTTTGTACTCTTTGAGTACCCTAAGGCTTACGAAGAATGTACTTTGAAATGTCAAGAATGTAGAAGAGCGTTTCAAGCTGTGACGATACCAAAACCTCCGGTGGATAAGAAAGACGACGAAGACGTTTACTTTTGCTCATGGGCTTTGTTTCCATTGGGGTTTTCCGGTGAATTTCAAGCTCCAAGTTGGTCACCTATTTCACCTCTCTTTGCCTGTCCTCTGCAAAAGTTGGATGGTGAACCAAAGAAGAGAAAGGAACCGGGGAAAGAAACATATGTGAGAAGTAACAAGAAACATGGAGTTGAAGAAGTCCAAAATGTAGAAAGTATCAGTAGTGCTAGCAATCACGCGGTTTCAATTGCTTCAAATGCTGCGGCTGTTGGGTCTTCAACTGGGGTTATGTCTAAACCAGGGAGTTATATTACTAGAAAACGAATGGGAACAGGCGCTAAGAACCTGGGGAGGTTGGATTTGAATGTGGAGTTTAGTAATGAAGTGGAAGAGCCTGGTGTGGCAGGTGGGGGAAATGCAGGCAATGGGATCGGAAGTAACAGGGAGGTAGATAATATGGAAGGGATTGGGTTCTTTGAGGGACTTGATGAATTCTTGAATAGTTTGCCCATACTTTCTGTTGTTGGAGATGATAAGATCAAGGCTACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

422

Amino Acids

45.86

Weight (kDa)

5.48

Isoelectric Point (pI)

44.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 79 - 141 1.1e-08 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1112
Acc36I ACCTGC 1 cut(s) 1112
AccI GTMKAC 1 cut(s) 304
AccII CGCG 5 cut(s) 15, 129, 290, 535, 956
AclWI GGATC 6 cut(s) 63, 118, 392, 560, 573, 1154
AcsI RAATTY 3 cut(s) 487, 776, 1208
AfaI GTAC 5 cut(s) 242, 299, 612, 623, 646
AfiI CCNNNNNNNGG 1 cut(s) 381
AflIII ACRYGT 1 cut(s) 283
AjiI CACGTC 1 cut(s) 286
AjnI CCWGG 4 cut(s) 546, 1015, 1062, 1111
AluBI AGCT 8 cut(s) 82, 140, 161, 269, 359, 582, 683, 785
AluI AGCT 8 cut(s) 82, 140, 161, 269, 359, 582, 683, 785
Alw21I GWGCWC 1 cut(s) 361
Alw26I GTCTC 4 cut(s) 200, 344, 376, 543
AlwI GGATC 6 cut(s) 63, 118, 392, 560, 573, 1154
AlwNI CAGNNNCTG 1 cut(s) 161
Ama87I CYCGRG 1 cut(s) 150
ApeKI GCWGC 2 cut(s) 161, 977
ApoI RAATTY 3 cut(s) 487, 776, 1208
Asp700I GAANNNNTTC 1 cut(s) 413
AspLEI GCGC 2 cut(s) 131, 1055
AspS9I GGNCC 2 cut(s) 555, 588
AsuC2I CCSGG 1 cut(s) 867
AsuHPI GGTGA 5 cut(s) 230, 785, 789, 798, 854
AsuNHI GCTAGC 1 cut(s) 944
AvaI CYCGRG 1 cut(s) 150
AvaII GGWCC 2 cut(s) 555, 588
AxyI CCTNAGG 1 cut(s) 627
BamHI GGATCC 1 cut(s) 565
BanII GRGCYC 1 cut(s) 361
BbsI GAAGAC 2 cut(s) 732, 984
Bbv12I GWGCWC 1 cut(s) 361
BbvI GCAGC 2 cut(s) 148, 964
BccI CCATC 2 cut(s) 471, 833
BceAI ACGGC 2 cut(s) 230, 503
BcgI CGANNNNNNTGC 2 cut(s) 1129, 1163
BciT130I CCWGG 4 cut(s) 548, 1017, 1064, 1113
BcnI CCSGG 1 cut(s) 867
BcoDI GTCTC 4 cut(s) 200, 344, 376, 543
BfaI CTAG 5 cut(s) 86, 93, 504, 945, 1032
BfmI CTRYAG 1 cut(s) 162
BfoI RGCGCY 1 cut(s) 1056
BfuAI ACCTGC 1 cut(s) 1112
BisI GCNGC 4 cut(s) 16, 162, 978, 981
BlsI GCNGC 4 cut(s) 17, 163, 979, 982
Bme1390I CCNGG 5 cut(s) 548, 867, 1017, 1064, 1113
Bme18I GGWCC 2 cut(s) 555, 588
BmeT110I CYCGRG 1 cut(s) 150
BmgBI CACGTC 1 cut(s) 286
BmgT120I GGNCC 2 cut(s) 555, 588
BmiI GGNNCC 4 cut(s) 501, 556, 567, 864
BmrFI CCNGG 5 cut(s) 548, 867, 1017, 1064, 1113
BmrI ACTGGG 1 cut(s) 1008
BmsI GCATC 2 cut(s) 367, 490
BmtI GCTAGC 1 cut(s) 948
BmuI ACTGGG 1 cut(s) 1008
BpiI GAAGAC 2 cut(s) 732, 984
BpuMI CCSGG 1 cut(s) 867
BsaI GGTCTC 1 cut(s) 543
BsaJI CCNNGG 5 cut(s) 533, 558, 866, 1016, 1063
BsaWI WCCGGW 2 cut(s) 703, 770
Bsc4I CCNNNNNNNGG 1 cut(s) 381
Bse1I ACTGG 3 cut(s) 242, 493, 1003
Bse21I CCTNAGG 1 cut(s) 627
Bse3DI GCAATG 1 cut(s) 1147
BseBI CCWGG 4 cut(s) 548, 1017, 1064, 1113
BseDI CCNNGG 5 cut(s) 533, 558, 866, 1016, 1063
BseGI GGATG 3 cut(s) 171, 463, 844
BseLI CCNNNNNNNGG 1 cut(s) 381
BseMI GCAATG 1 cut(s) 1147
BseMII CTCAG 2 cut(s) 308, 554
BseNI ACTGG 3 cut(s) 242, 493, 1003
BseRI GAGGAG 1 cut(s) 308
BseXI GCAGC 2 cut(s) 148, 964
BseYI CCCAGC 1 cut(s) 551
Bsh1236I CGCG 5 cut(s) 15, 129, 290, 535, 956
Bsh1285I CGRYCG 3 cut(s) 262, 355, 424
BsiEI CGRYCG 3 cut(s) 262, 355, 424
BsiHKAI GWGCWC 1 cut(s) 361
BsiHKCI CYCGRG 1 cut(s) 150
BsiSI CCGG 4 cut(s) 201, 704, 771, 866
BslFI GGGAC 2 cut(s) 568, 1212
BslI CCNNNNNNNGG 1 cut(s) 381
BsmAI GTCTC 4 cut(s) 200, 344, 376, 543
BsmBI CGTCTC 2 cut(s) 344, 376
BsmFI GGGAC 2 cut(s) 568, 1212
Bso31I GGTCTC 1 cut(s) 543
BsoBI CYCGRG 1 cut(s) 150
Bsp1286I GDGCHC 1 cut(s) 361
Bsp143I GATC 8 cut(s) 55, 97, 123, 352, 397, 565, 1146, 1254
Bsp68I TCGCGA 1 cut(s) 290
BspCNI CTCAG 2 cut(s) 307, 553
BspFNI CGCG 5 cut(s) 15, 129, 290, 535, 956
BspLI GGNNCC 4 cut(s) 501, 556, 567, 864
BspMAI CTGCAG 1 cut(s) 166
BspMI ACCTGC 1 cut(s) 1112
BspOI GCTAGC 1 cut(s) 948
BspPI GGATC 6 cut(s) 63, 118, 392, 560, 573, 1154
BspQI GCTCTTC 3 cut(s) 318, 664, 1101
BspTNI GGTCTC 1 cut(s) 543
BsrDI GCAATG 1 cut(s) 1147
BsrI ACTGG 3 cut(s) 242, 493, 1003
BssECI CCNNGG 5 cut(s) 533, 558, 866, 1016, 1063
BssMI GATC 8 cut(s) 55, 97, 123, 352, 397, 565, 1146, 1254
Bst2UI CCWGG 4 cut(s) 548, 1017, 1064, 1113
Bst4CI ACNGT 1 cut(s) 302
Bst6I CTCTTC 4 cut(s) 318, 664, 848, 1101
BstAPI GCANNNNNTGC 1 cut(s) 161
BstC8I GCNNGC 4 cut(s) 133, 595, 946, 1138
BstDEI CTNAG 6 cut(s) 294, 437, 540, 627, 1056, 1266
BstDSI CCRYGG 1 cut(s) 533
BstEII GGTNACC 1 cut(s) 795
BstF5I GGATG 3 cut(s) 171, 463, 844
BstFNI CGCG 5 cut(s) 15, 129, 290, 535, 956
BstH2I RGCGCY 1 cut(s) 1056
BstHHI GCGC 2 cut(s) 131, 1055
BstKTI GATC 8 cut(s) 58, 100, 126, 355, 400, 568, 1149, 1257
BstMAI GTCTC 4 cut(s) 200, 344, 376, 543
BstMBI GATC 8 cut(s) 55, 97, 123, 352, 397, 565, 1146, 1254
BstMCI CGRYCG 3 cut(s) 262, 355, 424
BstMWI GCNNNNNNNGC 9 cut(s) 15, 24, 128, 137, 161, 356, 603, 680, 974
BstNI CCWGG 4 cut(s) 548, 1017, 1064, 1113
BstNSI RCATGY 1 cut(s) 135
BstPI GGTNACC 1 cut(s) 795
BstSCI CCNGG 5 cut(s) 546, 865, 1015, 1062, 1111
BstSFI CTRYAG 1 cut(s) 162
BstUI CGCG 5 cut(s) 15, 129, 290, 535, 956
BstV1I GCAGC 2 cut(s) 148, 964
BstV2I GAAGAC 2 cut(s) 732, 984
BstX2I RGATCY 1 cut(s) 565
BstYI RGATCY 1 cut(s) 565
Bsu36I CCTNAGG 1 cut(s) 627
BtgI CCRYGG 1 cut(s) 533
BtrI CACGTC 1 cut(s) 286
BtsCI GGATG 3 cut(s) 171, 463, 844
BtsIMutI CAGTG 2 cut(s) 65, 500
BtuMI TCGCGA 1 cut(s) 290
BveI ACCTGC 1 cut(s) 1112
Cac8I GCNNGC 4 cut(s) 133, 595, 946, 1138
CaiI CAGNNNCTG 1 cut(s) 161
CfoI GCGC 2 cut(s) 131, 1055
Cfr13I GGNCC 2 cut(s) 555, 588
Cfr42I CCGCGG 1 cut(s) 536
CseI GACGC 2 cut(s) 21, 78
Csp6I GTAC 5 cut(s) 241, 298, 611, 622, 645
CviAII CATG 3 cut(s) 132, 744, 902
CviQI GTAC 5 cut(s) 241, 298, 611, 622, 645
DdeI CTNAG 6 cut(s) 294, 437, 540, 627, 1056, 1266
DpnI GATC 8 cut(s) 57, 99, 125, 354, 399, 567, 1148, 1256
DpnII GATC 8 cut(s) 55, 97, 123, 352, 397, 565, 1146, 1254
Eam1104I CTCTTC 4 cut(s) 318, 664, 848, 1101
EarI CTCTTC 4 cut(s) 318, 664, 848, 1101
EciI GGCGGA 5 cut(s) 24, 33, 68, 516, 578
Ecl136II GAGCTC 1 cut(s) 359
Eco24I GRGCYC 1 cut(s) 361
Eco31I GGTCTC 1 cut(s) 543
Eco47I GGWCC 2 cut(s) 555, 588
Eco53kI GAGCTC 1 cut(s) 359
Eco81I CCTNAGG 1 cut(s) 627
Eco88I CYCGRG 1 cut(s) 150
Eco91I GGTNACC 1 cut(s) 795
EcoICRI GAGCTC 1 cut(s) 359
EcoO109I RGGNCCY 1 cut(s) 555
EcoO65I GGTNACC 1 cut(s) 795
EcoRI GAATTC 1 cut(s) 1208
EcoRII CCWGG 4 cut(s) 546, 1015, 1062, 1111
EcoT38I GRGCYC 1 cut(s) 361
Esp3I CGTCTC 2 cut(s) 344, 376
FaeI CATG 3 cut(s) 135, 747, 905
FaqI GGGAC 2 cut(s) 568, 1212
FatI CATG 3 cut(s) 131, 743, 901
FauNDI CATATG 1 cut(s) 880
FblI GTMKAC 1 cut(s) 304
Fnu4HI GCNGC 4 cut(s) 16, 162, 978, 981
FokI GGATG 3 cut(s) 158, 450, 851
FriOI GRGCYC 1 cut(s) 361
Fsp4HI GCNGC 4 cut(s) 16, 162, 978, 981
FspBI CTAG 5 cut(s) 86, 93, 504, 945, 1032
GlaI GCGC 2 cut(s) 130, 1054
GluI GCNGC 4 cut(s) 16, 162, 978, 981
GsaI CCCAGC 1 cut(s) 555
HaeII RGCGCY 1 cut(s) 1056
HapII CCGG 4 cut(s) 201, 704, 771, 866
HgaI GACGC 2 cut(s) 21, 78
HhaI GCGC 2 cut(s) 131, 1055
Hin1II CATG 3 cut(s) 135, 747, 905
Hin6I GCGC 2 cut(s) 129, 1053
HinP1I GCGC 2 cut(s) 129, 1053
HincII GTYRAC 1 cut(s) 443
HindII GTYRAC 1 cut(s) 443
HindIII AAGCTT 1 cut(s) 80
HinfI GANTC 6 cut(s) 208, 221, 255, 292, 306, 459
HpaII CCGG 4 cut(s) 201, 704, 771, 866
HphI GGTGA 5 cut(s) 230, 785, 789, 798, 854
Hpy166II GTNNAC 4 cut(s) 305, 443, 733, 845
Hpy188I TCNGA 6 cut(s) 76, 254, 376, 397, 543, 1151
Hpy188III TCNNGA 6 cut(s) 289, 406, 456, 586, 659, 1213
Hpy8I GTNNAC 4 cut(s) 305, 443, 733, 845
Hpy99I CGWCG 3 cut(s) 290, 580, 725
HpyAV CCTTC 2 cut(s) 336, 1172
HpyCH4III ACNGT 1 cut(s) 302
HpyCH4IV ACGT 2 cut(s) 285, 729
HpyCH4V TGCA 4 cut(s) 164, 433, 829, 1136
HpyF10VI GCNNNNNNNGC 9 cut(s) 15, 24, 128, 137, 161, 356, 603, 680, 974
HpyF3I CTNAG 6 cut(s) 294, 437, 540, 627, 1056, 1266
HpySE526I ACGT 2 cut(s) 285, 729
Hsp92II CATG 3 cut(s) 135, 747, 905
HspAI GCGC 2 cut(s) 129, 1053
KspI CCGCGG 1 cut(s) 536
Kzo9I GATC 8 cut(s) 55, 97, 123, 352, 397, 565, 1146, 1254
LguI GCTCTTC 3 cut(s) 318, 664, 1101
LmnI GCTCC 2 cut(s) 107, 790
Lsp1109I GCAGC 2 cut(s) 148, 964
LweI GCATC 2 cut(s) 367, 490
MaeI CTAG 5 cut(s) 86, 93, 504, 945, 1032
MaeII ACGT 2 cut(s) 285, 729
MaeIII GTNAC 6 cut(s) 38, 67, 685, 795, 890, 1154
MalI GATC 8 cut(s) 57, 99, 125, 354, 399, 567, 1148, 1256
MboI GATC 8 cut(s) 55, 97, 123, 352, 397, 565, 1146, 1254
MfeI CAATTG 1 cut(s) 963
MflI RGATCY 1 cut(s) 565
MhlI GDGCHC 1 cut(s) 361
MluCI AATT 5 cut(s) 428, 487, 776, 963, 1208
MlyI GAGTC 4 cut(s) 202, 249, 286, 300
MmeI TCCRAC 5 cut(s) 249, 277, 816, 1053, 1222
MroXI GAANNNNTTC 1 cut(s) 413
MseI TTAA 2 cut(s) 333, 363
MspA1I CMGCKG 1 cut(s) 535
MspI CCGG 4 cut(s) 201, 704, 771, 866
MspR9I CCNGG 5 cut(s) 548, 867, 1017, 1064, 1113
MunI CAATTG 1 cut(s) 963
MvaI CCWGG 4 cut(s) 548, 1017, 1064, 1113
MvnI CGCG 5 cut(s) 15, 129, 290, 535, 956
MwoI GCNNNNNNNGC 9 cut(s) 15, 24, 128, 137, 161, 356, 603, 680, 974
NciI CCSGG 1 cut(s) 867
NdeI CATATG 1 cut(s) 880
NdeII GATC 8 cut(s) 55, 97, 123, 352, 397, 565, 1146, 1254
NheI GCTAGC 1 cut(s) 944
NlaIII CATG 3 cut(s) 135, 747, 905
NlaIV GGNNCC 4 cut(s) 501, 556, 567, 864
NmeAIII GCCGAG 1 cut(s) 539
NmuCI GTSAC 2 cut(s) 685, 795
NruI TCGCGA 1 cut(s) 290
NspI RCATGY 1 cut(s) 135
PaeI GCATGC 1 cut(s) 135
PaqCI CACCTGC 1 cut(s) 1112
PciSI GCTCTTC 3 cut(s) 318, 664, 1101
PcsI WCGNNNNNNNCGW 2 cut(s) 183, 726
PdmI GAANNNNTTC 1 cut(s) 413
PfeI GAWTC 2 cut(s) 221, 459
PflFI GACNNNGTC 1 cut(s) 258
PkrI GCNGC 4 cut(s) 17, 163, 979, 982
Ple19I CGATCG 1 cut(s) 355
PleI GAGTC 4 cut(s) 202, 249, 286, 300
PpsI GAGTC 4 cut(s) 202, 249, 286, 300
PpuMI RGGWCCY 1 cut(s) 555
Psp124BI GAGCTC 1 cut(s) 361
Psp5II RGGWCCY 1 cut(s) 555
Psp6I CCWGG 4 cut(s) 546, 1015, 1062, 1111
PspEI GGTNACC 1 cut(s) 795
PspFI CCCAGC 1 cut(s) 551
PspGI CCWGG 4 cut(s) 546, 1015, 1062, 1111
PspN4I GGNNCC 4 cut(s) 501, 556, 567, 864
PspPI GGNCC 2 cut(s) 555, 588
PspPPI RGGWCCY 1 cut(s) 555
PstI CTGCAG 1 cut(s) 166
PstNI CAGNNNCTG 1 cut(s) 161
PsuI RGATCY 1 cut(s) 565
PsyI GACNNNGTC 1 cut(s) 258
PvuI CGATCG 1 cut(s) 355
RruI TCGCGA 1 cut(s) 290
RsaI GTAC 5 cut(s) 242, 299, 612, 623, 646
RsaNI GTAC 5 cut(s) 241, 298, 611, 622, 645
SacI GAGCTC 1 cut(s) 361
SacII CCGCGG 1 cut(s) 536
SapI GCTCTTC 3 cut(s) 318, 664, 1101
SaqAI TTAA 2 cut(s) 333, 363
SatI GCNGC 4 cut(s) 16, 162, 978, 981
Sau3AI GATC 8 cut(s) 55, 97, 123, 352, 397, 565, 1146, 1254
Sau96I GGNCC 2 cut(s) 555, 588
SchI GAGTC 4 cut(s) 202, 249, 286, 300
ScrFI CCNGG 5 cut(s) 548, 867, 1017, 1064, 1113
SduI GDGCHC 1 cut(s) 361
SfaNI GCATC 2 cut(s) 367, 490
SfcI CTRYAG 1 cut(s) 162
Sfr303I CCGCGG 1 cut(s) 536
SgrBI CCGCGG 1 cut(s) 536
SinI GGWCC 2 cut(s) 555, 588
SphI GCATGC 1 cut(s) 135
Sse9I AATT 5 cut(s) 428, 487, 776, 963, 1208
SspMI CTAG 5 cut(s) 86, 93, 504, 945, 1032
SstI GAGCTC 1 cut(s) 361
StyD4I CCNGG 5 cut(s) 546, 865, 1015, 1062, 1111
TaaI ACNGT 1 cut(s) 302
TaiI ACGT 2 cut(s) 288, 732
TaqI TCGA 1 cut(s) 355
TaqII GACCGA 3 cut(s) 248, 437, 438
TasI AATT 5 cut(s) 428, 487, 776, 963, 1208
TatI WGTACW 2 cut(s) 610, 644
TauI GCSGC 2 cut(s) 18, 983
TfiI GAWTC 2 cut(s) 221, 459
Tru1I TTAA 2 cut(s) 333, 363
Tru9I TTAA 2 cut(s) 333, 363
TscAI CASTG 2 cut(s) 65, 500
TseFI GTSAC 2 cut(s) 685, 795
TseI GCWGC 2 cut(s) 161, 977
Tsp45I GTSAC 2 cut(s) 685, 795
TspDTI ATGAA 2 cut(s) 1113, 1221
TspGWI ACGGA 2 cut(s) 319, 390
TspRI CASTG 2 cut(s) 65, 500
Tth111I GACNNNGTC 1 cut(s) 258
VpaK11BI GGWCC 2 cut(s) 555, 588
XapI RAATTY 3 cut(s) 487, 776, 1208
XceI RCATGY 1 cut(s) 135
XmiI GTMKAC 1 cut(s) 304
XmnI GAANNNNTTC 1 cut(s) 413
XspI CTAG 5 cut(s) 86, 93, 504, 945, 1032
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.