MD10G1054200.v1.1

DnaJ molecular chaperone homology domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0009201
Physical Location & Seq
Reverse (-)
7356545 .. 7359150
2606 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1054200.v1.1.491

Sequence Viewer

Length: 1800 bp
ATGGAGTTCAATTTCAACACCAACAGGGCGGAAGCGGAGCGGTGGCTGAGCACCGCTGTGAAGCTACTGTCGGCGCATGACTTGCAGGGGACTAAGACCTTCGCGATCCGAGCCCGAGAGACCGACCCCAGGCTCGAGGCCACCGACCAGATAATCGCCGTCGCTGACACCCTGCTCGCCGCCGAATCCCAGATCAACAGCCAAAACCAGCAGCCCGACTGGTACGCTATTCTACAACTCGCCCAGTACACTCAGAACCTCGAAATCGTCGCGACTCAGTACCGCCGACTCGCTCTCCTGCTGAACCCCCACCGGAATCGCTTCCCTTACGCCGATCATGCCTTCCGGCTGGTCTCCGAGGCCTGGAACGTCCTCTCTAACCCCACCAAGAAGGCCATGTACGACCACGAGTTGAGCATGTTCAACCGGTTCGACTCGCCCCCATCCGGGTCGACTCAGCTGTTCGAGAGGCAATTCTTGCAAATGCATCATGTTCAGCAACAGCCGCAGCAGCCACCACCACCACAGCCACTTCTATTTCATCCCCAACCGTCACAGCTACTACAATTCCAGCCTCAACCGCCACCGCCGCCACAACCACAACCAGAAGTGCAGAGACCACAGCCACAATGGAATTTTCAACAAAAGCGACAACAACCACCACCAGAGCAGCAACATCAGCAGCAGCACCATCACCACGAACCTCCTCAGCAGCAACAATATCAGGAACTTCCCCAGCTGTGGCAGCAGCTGCAGCAACAACAGCAGCAACAAGAAGTGAGTAAAAACCCTAGAAGTAAGGACGGAAGACCGTCGATGGAGGAGGAAAGGCCAATTCCGATTCCCATCAATGTGACGGAGCCTGCACCTCCGGCCTCTGAGTCAACTCCTCCGCCCTCCGAATCAACTGGCCCTGTTGAGTCAACTCCTCCTTCTGAGTCAGCTCCCCCTTCTGAGTCGACTCGGCCTCAAACAGTGTCCAAAACGGGGAGCTTCTGGACTGCATGTCCTTACTGTTATAACCTCTTCGAGTATCCTAGCGCTTACGCGGATTGCATGCTCCGGTGCCAGAATTGTAAGAGGGCGTTCAATGCAATGGCGATAGCTGCACCGCCTCAGGCGGGGAAAGATGTGAACTTCTGCTGTTGGGGGTATTTCCCATTGGGGTTACTGGAGAATAGTAAGGATACAGGCGGGTCTGCTGAGGTGTGGACACCATTTTCGACAATGTTTGCTTGCCCAATACAAGGGAAGAAGAATACCGCACGAGCCAAGATTGCTAATTCGGGGCCAAGGGTCTATTATGATGATGAGGAGGCGTTGCTTGATGTTTCGGACCCAAGTGAGGACTCTGATGATGATGATGAGTGGCAGAGGGTTAGGAGGAAGAAGAGAGTTGTAAAGGCAAAAGGTAAAGCTTCCACCGCTAAGACTCCTGTGAGAGCTTCGGATAGATTAAGGAAGGGGAGTCAGAATGCTGGTGGGCAGGGTAAGGAGGGTACGGGTGGGAGTGTGGGTGCTGGGTCTGTGTCGAAGGCAGAGTCAAGTAAGAAATCAACTTCTGGTGCAAGAAAGAGAAGTGCTGCTGCATTGGGGAAGTTGGATTTGAATGTGGAGTTTAGTAATAATGAGGGGGAAGAGCCTGCGGCTCAGACAATGAGTGAAGGGAATGGGACAGCAAATGGGGAGGAGGATAATATGGAAGGGATCGGGTTTTTTGAAGGTCTTGACGAGTTTTTGAGTAGTCTACCCATACTTAATGTTGTGGGAGATGACAAGATGGTTTGGGATCGACTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

600

Amino Acids

66.99

Weight (kDa)

5.68

Isoelectric Point (pI)

62.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 73 - 135 4e-13 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1020
AccB1I GGYRCC 1 cut(s) 1065
AccBSI CCGCTC 1 cut(s) 40
AccI GTMKAC 3 cut(s) 452, 959, 1747
AccII CGCG 3 cut(s) 104, 272, 1049
AclWI GGATC 3 cut(s) 100, 1715, 1797
AcsI RAATTY 1 cut(s) 634
AfaI GTAC 5 cut(s) 224, 248, 281, 401, 1501
AfeI AGCGCT 1 cut(s) 1042
AfiI CCNNNNNNNGG 9 cut(s) 129, 363, 446, 447, 741, 987, 1121, 1247, 1345
AgeI ACCGGT 1 cut(s) 426
AgsI TTSAA 7 cut(s) 10, 16, 424, 641, 1090, 1609, 1721
AhdI GACNNNNNGTC 1 cut(s) 1005
AjnI CCWGG 2 cut(s) 128, 362
AleI CACNNNNGTG 1 cut(s) 56
Alw21I GWGCWC 1 cut(s) 53
Alw26I GTCTC 3 cut(s) 113, 358, 610
AlwI GGATC 3 cut(s) 100, 1715, 1797
AlwNI CAGNNNCTG 1 cut(s) 751
Ama87I CYCGRG 2 cut(s) 114, 134
Aor51HI AGCGCT 1 cut(s) 1042
AoxI GGCC 8 cut(s) 138, 360, 393, 830, 873, 910, 965, 1289
ApoI RAATTY 1 cut(s) 634
ArsI GACNNNNNNTTYG 4 cut(s) 1204, 1236, 1526, 1558
AsiGI ACCGGT 1 cut(s) 426
Asp700I GAANNNNTTC 1 cut(s) 320
AspLEI GCGC 2 cut(s) 76, 1043
AspS9I GGNCC 3 cut(s) 911, 1289, 1336
AsuC2I CCSGG 1 cut(s) 448
AsuHPI GGTGA 1 cut(s) 686
AvaI CYCGRG 2 cut(s) 114, 134
AvaII GGWCC 1 cut(s) 1336
AxyI CCTNAGG 1 cut(s) 1116
BanI GGYRCC 1 cut(s) 1065
BanII GRGCYC 1 cut(s) 115
BarI GAAGNNNNNNTAC 2 cut(s) 383, 415
BauI CACGAG 2 cut(s) 407, 1266
BbsI GAAGAC 1 cut(s) 814
Bbv12I GWGCWC 1 cut(s) 53
BbvCI CCTCAGC 2 cut(s) 708, 1203
BccI CCATC 5 cut(s) 451, 699, 811, 854, 1774
BceAI ACGGC 1 cut(s) 143
BciT130I CCWGG 2 cut(s) 130, 364
BciVI GTATCC 2 cut(s) 1044, 1180
BcnI CCSGG 1 cut(s) 448
BcoDI GTCTC 3 cut(s) 113, 358, 610
BfaI CTAG 2 cut(s) 792, 1038
BfmI CTRYAG 1 cut(s) 752
BfoI RGCGCY 1 cut(s) 1044
BfuI GTATCC 2 cut(s) 1044, 1180
BlpI GCTNAGC 1 cut(s) 47
Bme1390I CCNGG 3 cut(s) 130, 364, 448
Bme18I GGWCC 1 cut(s) 1336
BmeRI GACNNNNNGTC 1 cut(s) 1005
BmeT110I CYCGRG 2 cut(s) 114, 134
BmgT120I GGNCC 3 cut(s) 911, 1289, 1336
BmiI GGNNCC 4 cut(s) 861, 1067, 1290, 1338
BmrFI CCNGG 3 cut(s) 130, 364, 448
BmrI ACTGGG 1 cut(s) 238
BmsI GCATC 1 cut(s) 496
BmuI ACTGGG 1 cut(s) 238
BpiI GAAGAC 1 cut(s) 814
BpmI CTGGAG 1 cut(s) 1193
Bpu10I CCTNAGC 2 cut(s) 708, 1203
Bpu1102I GCTNAGC 1 cut(s) 47
BpuMI CCSGG 1 cut(s) 448
BsaBI GATNNNNATC 1 cut(s) 845
BsaI GGTCTC 3 cut(s) 113, 358, 610
BsaJI CCNNGG 3 cut(s) 128, 357, 1292
BsaWI WCCGGW 3 cut(s) 312, 426, 1062
BsaXI ACNNNNNCTCC 4 cut(s) 279, 309, 1501, 1531
Bsc4I CCNNNNNNNGG 9 cut(s) 129, 363, 446, 447, 741, 987, 1121, 1247, 1345
Bse118I RCCGGY 1 cut(s) 426
Bse1I ACTGG 4 cut(s) 224, 244, 913, 1176
Bse21I CCTNAGG 1 cut(s) 1116
Bse3DI GCAATG 1 cut(s) 1101
Bse8I GATNNNNATC 1 cut(s) 845
BseBI CCWGG 2 cut(s) 130, 364
BseDI CCNNGG 3 cut(s) 128, 357, 1292
BseGI GGATG 2 cut(s) 443, 541
BseJI GATNNNNATC 1 cut(s) 845
BseLI CCNNNNNNNGG 9 cut(s) 129, 363, 446, 447, 741, 987, 1121, 1247, 1345
BseMI GCAATG 1 cut(s) 1101
BseNI ACTGG 4 cut(s) 224, 244, 913, 1176
BseRI GAGGAG 6 cut(s) 696, 836, 879, 918, 1328, 1703
BseYI CCCAGC 2 cut(s) 735, 1520
BsgI GTGCAG 3 cut(s) 632, 849, 1092
Bsh1236I CGCG 3 cut(s) 104, 272, 1049
BshFI GGCC 8 cut(s) 140, 362, 395, 832, 875, 912, 967, 1291
BshNI GGYRCC 1 cut(s) 1065
BshTI ACCGGT 1 cut(s) 426
BsiHKAI GWGCWC 1 cut(s) 53
BsiHKCI CYCGRG 2 cut(s) 114, 134
BsiSI CCGG 6 cut(s) 313, 346, 427, 447, 872, 1063
BslFI GGGAC 2 cut(s) 103, 1687
BslI CCNNNNNNNGG 9 cut(s) 129, 363, 446, 447, 741, 987, 1121, 1247, 1345
BsmAI GTCTC 3 cut(s) 113, 358, 610
BsmFI GGGAC 2 cut(s) 103, 1687
BsmI GAATGC 1 cut(s) 1480
BsnI GGCC 8 cut(s) 140, 362, 395, 832, 875, 912, 967, 1291
Bso31I GGTCTC 3 cut(s) 113, 358, 610
BsoBI CYCGRG 2 cut(s) 114, 134
Bsp1286I GDGCHC 2 cut(s) 53, 115
Bsp143I GATC 5 cut(s) 105, 192, 334, 1707, 1789
Bsp1720I GCTNAGC 1 cut(s) 47
Bsp68I TCGCGA 2 cut(s) 104, 272
BspANI GGCC 8 cut(s) 140, 362, 395, 832, 875, 912, 967, 1291
BspFNI CGCG 3 cut(s) 104, 272, 1049
BspLI GGNNCC 4 cut(s) 861, 1067, 1290, 1338
BspMAI CTGCAG 1 cut(s) 756
BspPI GGATC 3 cut(s) 100, 1715, 1797
BspQI GCTCTTC 1 cut(s) 1632
BspT107I GGYRCC 1 cut(s) 1065
BspTNI GGTCTC 3 cut(s) 113, 358, 610
BsrBI CCGCTC 1 cut(s) 40
BsrDI GCAATG 1 cut(s) 1101
BsrFI RCCGGY 1 cut(s) 426
BsrI ACTGG 4 cut(s) 224, 244, 913, 1176
BssAI RCCGGY 1 cut(s) 426
BssECI CCNNGG 3 cut(s) 128, 357, 1292
BssMI GATC 5 cut(s) 105, 192, 334, 1707, 1789
BssSI CACGAG 2 cut(s) 407, 1266
BssT1I CCWWGG 1 cut(s) 1292
Bst2BI CACGAG 2 cut(s) 407, 1266
Bst2UI CCWGG 2 cut(s) 130, 364
Bst4CI ACNGT 5 cut(s) 69, 552, 813, 976, 1016
Bst6I CTCTTC 3 cut(s) 1031, 1385, 1632
BstAPI GCANNNNNTGC 3 cut(s) 82, 478, 751
BstC8I GCNNGC 5 cut(s) 177, 864, 1058, 1237, 1644
BstF5I GGATG 2 cut(s) 443, 541
BstFNI CGCG 3 cut(s) 104, 272, 1049
BstH2I RGCGCY 1 cut(s) 1044
BstHHI GCGC 2 cut(s) 76, 1043
BstKTI GATC 5 cut(s) 108, 195, 337, 1710, 1792
BstMAI GTCTC 3 cut(s) 113, 358, 610
BstMBI GATC 5 cut(s) 105, 192, 334, 1707, 1789
BstNI CCWGG 2 cut(s) 130, 364
BstNSI RCATGY 3 cut(s) 421, 1008, 1060
BstSCI CCNGG 3 cut(s) 128, 362, 446
BstSFI CTRYAG 1 cut(s) 752
BstUI CGCG 3 cut(s) 104, 272, 1049
BstV2I GAAGAC 1 cut(s) 814
Bsu36I CCTNAGG 1 cut(s) 1116
BsuI GTATCC 2 cut(s) 1044, 1180
BsuRI GGCC 8 cut(s) 140, 362, 395, 832, 875, 912, 967, 1291
BtsCI GGATG 2 cut(s) 443, 541
BtsIMutI CAGTG 1 cut(s) 981
BtuMI TCGCGA 2 cut(s) 104, 272
Cac8I GCNNGC 5 cut(s) 177, 864, 1058, 1237, 1644
CaiI CAGNNNCTG 1 cut(s) 751
CfoI GCGC 2 cut(s) 76, 1043
Cfr10I RCCGGY 1 cut(s) 426
Cfr13I GGNCC 3 cut(s) 911, 1289, 1336
Csp6I GTAC 5 cut(s) 223, 247, 280, 400, 1500
CspAI ACCGGT 1 cut(s) 426
CviAII CATG 7 cut(s) 77, 338, 397, 418, 491, 1005, 1057
CviQI GTAC 5 cut(s) 223, 247, 280, 400, 1500
DpnI GATC 5 cut(s) 107, 194, 336, 1709, 1791
DpnII GATC 5 cut(s) 105, 192, 334, 1707, 1789
DriI GACNNNNNGTC 1 cut(s) 1005
Eam1104I CTCTTC 3 cut(s) 1031, 1385, 1632
Eam1105I GACNNNNNGTC 1 cut(s) 1005
EarI CTCTTC 3 cut(s) 1031, 1385, 1632
EciI GGCGGA 2 cut(s) 44, 882
Eco130I CCWWGG 1 cut(s) 1292
Eco147I AGGCCT 1 cut(s) 362
Eco24I GRGCYC 1 cut(s) 115
Eco31I GGTCTC 3 cut(s) 113, 358, 610
Eco47I GGWCC 1 cut(s) 1336
Eco47III AGCGCT 1 cut(s) 1042
Eco81I CCTNAGG 1 cut(s) 1116
Eco88I CYCGRG 2 cut(s) 114, 134
EcoRII CCWGG 2 cut(s) 128, 362
EcoT14I CCWWGG 1 cut(s) 1292
EcoT22I ATGCAT 1 cut(s) 489
EcoT38I GRGCYC 1 cut(s) 115
ErhI CCWWGG 1 cut(s) 1292
FaeI CATG 7 cut(s) 80, 341, 400, 421, 494, 1008, 1060
FaqI GGGAC 2 cut(s) 103, 1687
FatI CATG 7 cut(s) 76, 337, 396, 417, 490, 1004, 1056
FauI CCCGC 2 cut(s) 1114, 1187
FblI GTMKAC 3 cut(s) 452, 959, 1747
FokI GGATG 2 cut(s) 430, 528
FriOI GRGCYC 1 cut(s) 115
FspBI CTAG 2 cut(s) 792, 1038
GlaI GCGC 2 cut(s) 75, 1042
GsaI CCCAGC 2 cut(s) 739, 1524
GsuI CTGGAG 1 cut(s) 1193
HaeII RGCGCY 1 cut(s) 1044
HaeIII GGCC 8 cut(s) 140, 362, 395, 832, 875, 912, 967, 1291
HapII CCGG 6 cut(s) 313, 346, 427, 447, 872, 1063
HhaI GCGC 2 cut(s) 76, 1043
Hin1II CATG 7 cut(s) 80, 341, 400, 421, 494, 1008, 1060
Hin6I GCGC 2 cut(s) 74, 1041
HinP1I GCGC 2 cut(s) 74, 1041
HincII GTYRAC 4 cut(s) 453, 885, 924, 960
HindII GTYRAC 4 cut(s) 453, 885, 924, 960
HindIII AAGCTT 1 cut(s) 1416
HpaII CCGG 6 cut(s) 313, 346, 427, 447, 872, 1063
HphI GGTGA 1 cut(s) 686
Hpy166II GTNNAC 8 cut(s) 249, 453, 885, 924, 960, 1135, 1212, 1748
Hpy188III TCNNGA 6 cut(s) 103, 271, 466, 725, 997, 1727
Hpy8I GTNNAC 8 cut(s) 249, 453, 885, 924, 960, 1135, 1212, 1748
Hpy99I CGWCG 3 cut(s) 164, 272, 817
HpyCH4III ACNGT 5 cut(s) 69, 552, 813, 976, 1016
HpyCH4IV ACGT 1 cut(s) 369
HpySE526I ACGT 1 cut(s) 369
Hsp92II CATG 7 cut(s) 80, 341, 400, 421, 494, 1008, 1060
HspAI GCGC 2 cut(s) 74, 1041
Kzo9I GATC 5 cut(s) 105, 192, 334, 1707, 1789
LguI GCTCTTC 1 cut(s) 1632
LmnI GCTCC 5 cut(s) 37, 859, 949, 990, 1065
LweI GCATC 1 cut(s) 496
MaeI CTAG 2 cut(s) 792, 1038
MaeII ACGT 1 cut(s) 369
MaeIII GTNAC 3 cut(s) 552, 853, 1167
MalI GATC 5 cut(s) 107, 194, 336, 1709, 1791
MbiI CCGCTC 1 cut(s) 40
MboI GATC 5 cut(s) 105, 192, 334, 1707, 1789
MboII GAAGA 7 cut(s) 819, 1018, 1264, 1267, 1399, 1402, 1649
MhlI GDGCHC 2 cut(s) 53, 115
MluCI AATT 7 cut(s) 10, 473, 566, 634, 834, 1072, 1282
MmeI TCCRAC 1 cut(s) 1581
Mph1103I ATGCAT 1 cut(s) 489
MroXI GAANNNNTTC 1 cut(s) 320
MseI TTAA 2 cut(s) 1457, 1758
MslI CAYNNNNRTG 2 cut(s) 56, 851
MspA1I CMGCKG 4 cut(s) 56, 460, 739, 751
MspI CCGG 6 cut(s) 313, 346, 427, 447, 872, 1063
MspR9I CCNGG 3 cut(s) 130, 364, 448
Mva1269I GAATGC 1 cut(s) 1480
MvaI CCWGG 2 cut(s) 130, 364
MvnI CGCG 3 cut(s) 104, 272, 1049
NciI CCSGG 1 cut(s) 448
NdeII GATC 5 cut(s) 105, 192, 334, 1707, 1789
NlaIII CATG 7 cut(s) 80, 341, 400, 421, 494, 1008, 1060
NlaIV GGNNCC 4 cut(s) 861, 1067, 1290, 1338
NmeAIII GCCGAG 1 cut(s) 943
NmuCI GTSAC 2 cut(s) 552, 853
NruI TCGCGA 2 cut(s) 104, 272
NsiI ATGCAT 1 cut(s) 489
NspI RCATGY 3 cut(s) 421, 1008, 1060
OliI CACNNNNGTG 1 cut(s) 56
PaeI GCATGC 1 cut(s) 1060
PaeR7I CTCGAG 1 cut(s) 134
PceI AGGCCT 1 cut(s) 362
PciSI GCTCTTC 1 cut(s) 1632
PcsI WCGNNNNNNNCGW 1 cut(s) 141
PctI GAATGC 1 cut(s) 1480
PdmI GAANNNNTTC 1 cut(s) 320
PfeI GAWTC 4 cut(s) 185, 316, 841, 902
PinAI ACCGGT 1 cut(s) 426
PsiI TTATAA 1 cut(s) 1020
Psp6I CCWGG 2 cut(s) 128, 362
PspFI CCCAGC 2 cut(s) 735, 1520
PspGI CCWGG 2 cut(s) 128, 362
PspN4I GGNNCC 4 cut(s) 861, 1067, 1290, 1338
PspPI GGNCC 3 cut(s) 911, 1289, 1336
PspXI VCTCGAGB 1 cut(s) 134
PstI CTGCAG 1 cut(s) 756
PstNI CAGNNNCTG 1 cut(s) 751
PvuII CAGCTG 3 cut(s) 460, 739, 751
RruI TCGCGA 2 cut(s) 104, 272
RsaI GTAC 5 cut(s) 224, 248, 281, 401, 1501
RsaNI GTAC 5 cut(s) 223, 247, 280, 400, 1500
RseI CAYNNNNRTG 2 cut(s) 56, 851
SalI GTCGAC 2 cut(s) 451, 958
SapI GCTCTTC 1 cut(s) 1632
SaqAI TTAA 2 cut(s) 1457, 1758
Sau3AI GATC 5 cut(s) 105, 192, 334, 1707, 1789
Sau96I GGNCC 3 cut(s) 911, 1289, 1336
ScrFI CCNGG 3 cut(s) 130, 364, 448
SduI GDGCHC 2 cut(s) 53, 115
SfaNI GCATC 1 cut(s) 496
SfcI CTRYAG 1 cut(s) 752
Sfr274I CTCGAG 1 cut(s) 134
SinI GGWCC 1 cut(s) 1336
SlaI CTCGAG 1 cut(s) 134
SmiMI CAYNNNNRTG 2 cut(s) 56, 851
SmlI CTYRAG 1 cut(s) 134
SmoI CTYRAG 1 cut(s) 134
SphI GCATGC 1 cut(s) 1060
Sse9I AATT 7 cut(s) 10, 473, 566, 634, 834, 1072, 1282
SseBI AGGCCT 1 cut(s) 362
SspMI CTAG 2 cut(s) 792, 1038
StuI AGGCCT 1 cut(s) 362
StyD4I CCNGG 3 cut(s) 128, 362, 446
StyI CCWWGG 1 cut(s) 1292
TaaI ACNGT 5 cut(s) 69, 552, 813, 976, 1016
TaiI ACGT 1 cut(s) 372
TaqII GACCGA 1 cut(s) 137
TasI AATT 7 cut(s) 10, 473, 566, 634, 834, 1072, 1282
TatI WGTACW 1 cut(s) 246
TauI GCSGC 4 cut(s) 182, 508, 592, 1649
TfiI GAWTC 4 cut(s) 185, 316, 841, 902
Tru1I TTAA 2 cut(s) 1457, 1758
Tru9I TTAA 2 cut(s) 1457, 1758
TscAI CASTG 1 cut(s) 981
TseFI GTSAC 2 cut(s) 552, 853
Tsp45I GTSAC 2 cut(s) 552, 853
TspDTI ATGAA 1 cut(s) 530
TspGWI ACGGA 2 cut(s) 819, 872
TspRI CASTG 1 cut(s) 981
VpaK11BI GGWCC 1 cut(s) 1336
XapI RAATTY 1 cut(s) 634
XceI RCATGY 3 cut(s) 421, 1008, 1060
XcmI CCANNNNNNNNNTGG 1 cut(s) 627
XhoI CTCGAG 1 cut(s) 134
XmiI GTMKAC 3 cut(s) 452, 959, 1747
XmnI GAANNNNTTC 1 cut(s) 320
XspI CTAG 2 cut(s) 792, 1038
Zsp2I ATGCAT 1 cut(s) 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.