AT5G65220

ribosomal protein L29

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0013530
Physical Location & Seq
Forward (+)
26061165 .. 26062682
1518 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G65220.1

Sequence Viewer

Length: 522 bp
ATGCTTAGTCTCTCAATCGCTACGCCGGGGACGGCGGCGATTTTTCGTAGAGGAACTGCTTCGGCGACTTCAACTTCGTCTTCATTCCATGGTGTCAGAATCCAGCACCAGGTTTCTGCTCGCGTCCCCGCGGCGGCGACGATATCGTCGTCGTCTCCTAAACCGTCGGTGGTGATGATGTCGAAAAGAGAGGCGGAATTGAAAGAGATAAGATCGAAGACGACGGAGCAGTTACAAGAGGAGGTTGTTGACCTTAAAGGTGAGCTCTTTATGCTTCGTCTCCAGAAATCGGCAAGGAATGAGTTCAAATCTAGCGACTTTCGTCGTATGAAGAAACAAGTTGCTCGGATGTTGACGGTTAAAAGAGAGAGGGAGATCAAAGAAGGGATAAAGAAAAGGTTGTCGAGGAAACTTGACAGACAATGGAAGAAAAGCATAGTACCAAGACCACCTCCGTCTCTGAAGAAACTTCAAGAAGAAGAAGCTGCAGAAGAAGCAGCTGAAGCTGCTAAATCTGCTTGA

Protein Analysis

173

Amino Acids

19.38

Weight (kDa)

10.5

Isoelectric Point (pI)

58.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L29 PF00831 68 - 124 2.4e-14 Ribosomal L29 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013530)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G65220
fragaria_vesca FvH4_1g20710
malus_domestica MD00G1199600.v1.1 MD01G1031300.v1.1
prunus_persica Prupe.6G188000_v2.0.a1
pyrus_communis pycom01g05890
rosa_chinensis RchiOBHm_Chr2g0113161
rosa_laevigata RLG00000018048
rosa_multiflora Rmu_sc0006838.1_g000024 Rmu_sc0035384.1_g000001
rosa_roxburghii Rroxscaffold_2G00129560 Rroxscaffold_2G00129750
rosa_rugosa Rorug02G0185900
rosa_samantha Rh2AG240200 Rh2BG254100 Rh2CG244200 Rh2DG248100
rosa_wichuraiana Rw2G018800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 145
AccII CGCG 2 cut(s) 123, 131
AciI CCGC 5 cut(s) 35, 129, 131, 134, 194
AcuI CTGAAG 2 cut(s) 482, 522
AfaI GTAC 1 cut(s) 441
AfiI CCNNNNNNNGG 3 cut(s) 109, 133, 289
AgsI TTSAA 4 cut(s) 72, 202, 307, 473
AjnI CCWGG 1 cut(s) 108
AluBI AGCT 4 cut(s) 265, 485, 500, 506
AluI AGCT 4 cut(s) 265, 485, 500, 506
Alw21I GWGCWC 1 cut(s) 267
Alw26I GTCTC 4 cut(s) 14, 159, 284, 462
ApeKI GCWGC 3 cut(s) 485, 497, 506
ArsI GACNNNNNNTTYG 2 cut(s) 58, 90
Asp700I GAANNNNTTC 2 cut(s) 58, 302
AsuC2I CCSGG 1 cut(s) 27
AsuHPI GGTGA 2 cut(s) 184, 272
BanII GRGCYC 1 cut(s) 267
BbsI GAAGAC 2 cut(s) 72, 224
Bbv12I GWGCWC 1 cut(s) 267
BbvI GCAGC 3 cut(s) 472, 493, 509
BceAI ACGGC 1 cut(s) 48
BciT130I CCWGG 1 cut(s) 110
BcnI CCSGG 1 cut(s) 27
BcoDI GTCTC 4 cut(s) 14, 159, 284, 462
BfaI CTAG 1 cut(s) 312
BfmI CTRYAG 1 cut(s) 486
BisI GCNGC 6 cut(s) 36, 132, 135, 486, 498, 507
BlsI GCNGC 6 cut(s) 37, 133, 136, 487, 499, 508
Bme1390I CCNGG 2 cut(s) 27, 110
BmrFI CCNGG 2 cut(s) 27, 110
BoxI GACNNNNGTC 1 cut(s) 321
BpiI GAAGAC 2 cut(s) 72, 224
BpmI CTGGAG 1 cut(s) 266
BpuMI CCSGG 1 cut(s) 27
BsaJI CCNNGG 3 cut(s) 26, 88, 129
Bsc4I CCNNNNNNNGG 3 cut(s) 109, 133, 289
BseBI CCWGG 1 cut(s) 110
BseDI CCNNGG 3 cut(s) 26, 88, 129
BseGI GGATG 1 cut(s) 354
BseLI CCNNNNNNNGG 3 cut(s) 109, 133, 289
BseRI GAGGAG 1 cut(s) 254
BseXI GCAGC 3 cut(s) 472, 493, 509
Bsh1236I CGCG 2 cut(s) 123, 131
BsiHKAI GWGCWC 1 cut(s) 267
BsiSI CCGG 1 cut(s) 26
BslFI GGGAC 2 cut(s) 43, 110
BslI CCNNNNNNNGG 3 cut(s) 109, 133, 289
BsmAI GTCTC 4 cut(s) 14, 159, 284, 462
BsmBI CGTCTC 3 cut(s) 159, 284, 462
BsmFI GGGAC 2 cut(s) 43, 110
Bsp1286I GDGCHC 1 cut(s) 267
Bsp143I GATC 2 cut(s) 212, 375
Bsp19I CCATGG 1 cut(s) 88
BspACI CCGC 5 cut(s) 35, 129, 131, 134, 194
BspFNI CGCG 2 cut(s) 123, 131
BspMAI CTGCAG 1 cut(s) 490
BssECI CCNNGG 3 cut(s) 26, 88, 129
BssMI GATC 2 cut(s) 212, 375
BssT1I CCWWGG 1 cut(s) 88
Bst2UI CCWGG 1 cut(s) 110
Bst4CI ACNGT 2 cut(s) 165, 358
BstC8I GCNNGC 1 cut(s) 121
BstDEI CTNAG 1 cut(s) 5
BstDSI CCRYGG 2 cut(s) 88, 129
BstF5I GGATG 1 cut(s) 354
BstFNI CGCG 2 cut(s) 123, 131
BstKTI GATC 2 cut(s) 215, 378
BstMAI GTCTC 4 cut(s) 14, 159, 284, 462
BstMBI GATC 2 cut(s) 212, 375
BstMWI GCNNNNNNNGC 5 cut(s) 271, 494, 503, 506, 515
BstNI CCWGG 1 cut(s) 110
BstPAI GACNNNNGTC 1 cut(s) 321
BstSCI CCNGG 2 cut(s) 25, 108
BstSFI CTRYAG 1 cut(s) 486
BstUI CGCG 2 cut(s) 123, 131
BstV1I GCAGC 3 cut(s) 472, 493, 509
BstV2I GAAGAC 2 cut(s) 72, 224
BtgI CCRYGG 2 cut(s) 88, 129
BtsCI GGATG 1 cut(s) 354
Cac8I GCNNGC 1 cut(s) 121
Cfr42I CCGCGG 1 cut(s) 132
CseI GACGC 1 cut(s) 112
CsiI ACCWGGT 1 cut(s) 108
Csp6I GTAC 1 cut(s) 440
CviAII CATG 1 cut(s) 89
CviJI RGCY 4 cut(s) 265, 485, 500, 506
CviKI_1 RGCY 4 cut(s) 265, 485, 500, 506
CviQI GTAC 1 cut(s) 440
DdeI CTNAG 1 cut(s) 5
DpnI GATC 2 cut(s) 214, 377
DpnII GATC 2 cut(s) 212, 375
DrdI GACNNNNNNGTC 1 cut(s) 145
DseDI GACNNNNNNGTC 1 cut(s) 145
EciI GGCGGA 1 cut(s) 209
Ecl136II GAGCTC 1 cut(s) 265
Eco130I CCWWGG 1 cut(s) 88
Eco24I GRGCYC 1 cut(s) 267
Eco32I GATATC 1 cut(s) 144
Eco53kI GAGCTC 1 cut(s) 265
Eco57I CTGAAG 2 cut(s) 482, 522
EcoICRI GAGCTC 1 cut(s) 265
EcoRII CCWGG 1 cut(s) 108
EcoRV GATATC 1 cut(s) 144
EcoT14I CCWWGG 1 cut(s) 88
EcoT38I GRGCYC 1 cut(s) 267
ErhI CCWWGG 1 cut(s) 88
Esp3I CGTCTC 3 cut(s) 159, 284, 462
FaeI CATG 1 cut(s) 92
FaiI YATR 4 cut(s) 90, 272, 329, 437
FaqI GGGAC 2 cut(s) 43, 110
FatI CATG 1 cut(s) 88
FauI CCCGC 1 cut(s) 136
Fnu4HI GCNGC 6 cut(s) 36, 132, 135, 486, 498, 507
FokI GGATG 1 cut(s) 361
FriOI GRGCYC 1 cut(s) 267
Fsp4HI GCNGC 6 cut(s) 36, 132, 135, 486, 498, 507
FspBI CTAG 1 cut(s) 312
GluI GCNGC 6 cut(s) 36, 132, 135, 486, 498, 507
GsuI CTGGAG 1 cut(s) 266
HapII CCGG 1 cut(s) 26
HgaI GACGC 1 cut(s) 112
Hin1II CATG 1 cut(s) 92
HincII GTYRAC 2 cut(s) 250, 354
HindII GTYRAC 2 cut(s) 250, 354
HinfI GANTC 1 cut(s) 99
HpaII CCGG 1 cut(s) 26
HphI GGTGA 2 cut(s) 184, 272
Hpy166II GTNNAC 2 cut(s) 250, 354
Hpy188I TCNGA 3 cut(s) 98, 348, 462
Hpy188III TCNNGA 2 cut(s) 283, 473
Hpy8I GTNNAC 2 cut(s) 250, 354
Hpy99I CGWCG 6 cut(s) 142, 151, 154, 169, 226, 327
HpyAV CCTTC 1 cut(s) 377
HpyCH4III ACNGT 2 cut(s) 165, 358
HpyCH4V TGCA 1 cut(s) 488
HpyF10VI GCNNNNNNNGC 5 cut(s) 271, 494, 503, 506, 515
HpyF3I CTNAG 1 cut(s) 5
Hsp92II CATG 1 cut(s) 92
KspI CCGCGG 1 cut(s) 132
Kzo9I GATC 2 cut(s) 212, 375
LmnI GCTCC 1 cut(s) 226
LpnPI CCDG 5 cut(s) 39, 95, 116, 122, 296
Lsp1109I GCAGC 3 cut(s) 472, 493, 509
MabI ACCWGGT 1 cut(s) 108
MaeI CTAG 1 cut(s) 312
MaeIII GTNAC 1 cut(s) 231
MalI GATC 2 cut(s) 214, 377
MboI GATC 2 cut(s) 212, 375
MboII GAAGA 8 cut(s) 72, 229, 343, 439, 475, 488, 491, 503
MhlI GDGCHC 1 cut(s) 267
MluCI AATT 1 cut(s) 197
MnlI CCTC 7 cut(s) 44, 184, 232, 235, 363, 399, 462
MroXI GAANNNNTTC 2 cut(s) 58, 302
MseI TTAA 2 cut(s) 255, 360
MspA1I CMGCKG 2 cut(s) 131, 500
MspI CCGG 1 cut(s) 26
MspR9I CCNGG 2 cut(s) 27, 110
MvaI CCWGG 1 cut(s) 110
MvnI CGCG 2 cut(s) 123, 131
MwoI GCNNNNNNNGC 5 cut(s) 271, 494, 503, 506, 515
NciI CCSGG 1 cut(s) 27
NcoI CCATGG 1 cut(s) 88
NdeII GATC 2 cut(s) 212, 375
NlaIII CATG 1 cut(s) 92
PcsI WCGNNNNNNNCGW 1 cut(s) 146
PdmI GAANNNNTTC 2 cut(s) 58, 302
PfeI GAWTC 1 cut(s) 99
PkrI GCNGC 6 cut(s) 37, 133, 136, 487, 499, 508
PshAI GACNNNNGTC 1 cut(s) 321
Psp124BI GAGCTC 1 cut(s) 267
Psp6I CCWGG 1 cut(s) 108
PspGI CCWGG 1 cut(s) 108
PstI CTGCAG 1 cut(s) 490
PvuII CAGCTG 1 cut(s) 500
RsaI GTAC 1 cut(s) 441
RsaNI GTAC 1 cut(s) 440
SacI GAGCTC 1 cut(s) 267
SacII CCGCGG 1 cut(s) 132
SaqAI TTAA 2 cut(s) 255, 360
SatI GCNGC 6 cut(s) 36, 132, 135, 486, 498, 507
Sau3AI GATC 2 cut(s) 212, 375
ScrFI CCNGG 2 cut(s) 27, 110
SduI GDGCHC 1 cut(s) 267
SexAI ACCWGGT 1 cut(s) 108
SfcI CTRYAG 1 cut(s) 486
Sfr303I CCGCGG 1 cut(s) 132
SgrBI CCGCGG 1 cut(s) 132
Sse9I AATT 1 cut(s) 197
SsiI CCGC 5 cut(s) 35, 129, 131, 134, 194
SspMI CTAG 1 cut(s) 312
SstI GAGCTC 1 cut(s) 267
StyD4I CCNGG 2 cut(s) 25, 108
StyI CCWWGG 1 cut(s) 88
TaaI ACNGT 2 cut(s) 165, 358
TaqI TCGA 3 cut(s) 182, 215, 404
TasI AATT 1 cut(s) 197
TauI GCSGC 3 cut(s) 38, 134, 137
TfiI GAWTC 1 cut(s) 99
Tru1I TTAA 2 cut(s) 255, 360
Tru9I TTAA 2 cut(s) 255, 360
TseI GCWGC 3 cut(s) 485, 497, 506
TspDTI ATGAA 2 cut(s) 72, 344
TspGWI ACGGA 2 cut(s) 239, 444
XmnI GAANNNNTTC 2 cut(s) 58, 302
XspI CTAG 1 cut(s) 312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.