MD00G1199600.v1.1

50S ribosomal protein L29

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0013530
Physical Location & Seq
Reverse (-)
47097333 .. 47098710
1378 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1199600.v1.1.491

Sequence Viewer

Length: 525 bp
ATGTTGGGCGCAATCTCCATAGCTCCACCTTCAACGGTTACATTTCCCTCAAACCTGCCGTCGCCAGTCTCCAAGTCCTTCAATGGCATGCGAGTCCAACGCCTTTGCCCAAACATCGGCGTTCGTCTTCCGACGGAGTCATCTCGGAGGCCGTGCTCGCCGTCGTCTTCGGTTGTGATGATGGCCAAGAGAGAGGAAGAAATGAAGGAAATCCGGACCAAAACCACCGAAGAAATCAACGAGGAGGTGGTCGACCTCAAAGGAGAGCTCTTCATGCTTCGCCTCCAGAAATCGGCTCGCAACGAGTTCACTTCAAGCGAGTTCCGTCGAATGCGCAAAAGGATTGCTCGCTTGCTTACTGTTAAACGGGAAAGAGAGATTGAGGAGGGAATTGGTAAGAGGCTATCAAGAAAGTTCGATCGACAATGGAAGAAAAGCATTGTTGTTAGACCGCCTCCGTCGTTGAAGAAGTTGCAAGACGAAGAAGCAGCTGCAGAAGCTGCTGAGAAGGCTGCATCTGCATGA

Protein Analysis

175

Amino Acids

19.74

Weight (kDa)

10.16

Isoelectric Point (pI)

78.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L29 PF00831 69 - 125 3.2e-14 Ribosomal L29 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013530)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G65220
fragaria_vesca FvH4_1g20710
malus_domestica MD00G1199600.v1.1 MD01G1031300.v1.1
prunus_persica Prupe.6G188000_v2.0.a1
pyrus_communis pycom01g05890
rosa_chinensis RchiOBHm_Chr2g0113161
rosa_laevigata RLG00000018048
rosa_multiflora Rmu_sc0006838.1_g000024 Rmu_sc0035384.1_g000001
rosa_roxburghii Rroxscaffold_2G00129560 Rroxscaffold_2G00129750
rosa_rugosa Rorug02G0185900
rosa_samantha Rh2AG240200 Rh2BG254100 Rh2CG244200 Rh2DG248100
rosa_wichuraiana Rw2G018800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 335
Acc36I ACCTGC 1 cut(s) 63
AccI GTMKAC 1 cut(s) 252
AccIII TCCGGA 1 cut(s) 213
AciI CCGC 1 cut(s) 452
AcoI YGGCCR 1 cut(s) 183
AfiI CCNNNNNNNGG 2 cut(s) 116, 292
AgsI TTSAA 4 cut(s) 33, 82, 315, 466
AluBI AGCT 4 cut(s) 23, 268, 491, 500
AluI AGCT 4 cut(s) 23, 268, 491, 500
Alw21I GWGCWC 2 cut(s) 158, 270
Alw26I GTCTC 1 cut(s) 73
AlwNI CAGNNNCTG 1 cut(s) 500
Aor13HI TCCGGA 1 cut(s) 213
AoxI GGCC 2 cut(s) 149, 183
ApeKI GCWGC 4 cut(s) 488, 491, 500, 512
ArsI GACNNNNNNTTYG 2 cut(s) 44, 76
AspLEI GCGC 2 cut(s) 11, 336
AspS9I GGNCC 1 cut(s) 216
AvaII GGWCC 1 cut(s) 216
BalI TGGCCA 1 cut(s) 185
BanII GRGCYC 1 cut(s) 270
BbsI GAAGAC 2 cut(s) 119, 159
Bbv12I GWGCWC 2 cut(s) 158, 270
BbvI GCAGC 4 cut(s) 478, 487, 499, 500
BccI CCATC 1 cut(s) 175
BceAI ACGGC 3 cut(s) 43, 136, 145
BcoDI GTCTC 1 cut(s) 73
BfmI CTRYAG 1 cut(s) 492
BfuAI ACCTGC 1 cut(s) 63
BisI GCNGC 4 cut(s) 489, 492, 501, 513
BlsI GCNGC 4 cut(s) 490, 493, 502, 514
Bme18I GGWCC 1 cut(s) 216
BmgT120I GGNCC 1 cut(s) 216
BpiI GAAGAC 2 cut(s) 119, 159
BpmI CTGGAG 1 cut(s) 269
BsaWI WCCGGW 1 cut(s) 213
Bsc4I CCNNNNNNNGG 2 cut(s) 116, 292
Bse1I ACTGG 1 cut(s) 65
BseAI TCCGGA 1 cut(s) 213
BseLI CCNNNNNNNGG 2 cut(s) 116, 292
BseMII CTCAG 1 cut(s) 495
BseNI ACTGG 1 cut(s) 65
BseRI GAGGAG 2 cut(s) 257, 398
BseXI GCAGC 4 cut(s) 478, 487, 499, 500
Bsh1285I CGRYCG 1 cut(s) 421
BshFI GGCC 2 cut(s) 151, 185
BsiEI CGRYCG 1 cut(s) 421
BsiHKAI GWGCWC 2 cut(s) 158, 270
BsiSI CCGG 1 cut(s) 214
BslI CCNNNNNNNGG 2 cut(s) 116, 292
BsmAI GTCTC 1 cut(s) 73
BsmI GAATGC 1 cut(s) 336
BsnI GGCC 2 cut(s) 151, 185
Bsp1286I GDGCHC 2 cut(s) 158, 270
Bsp13I TCCGGA 1 cut(s) 213
Bsp143I GATC 1 cut(s) 418
BspACI CCGC 1 cut(s) 452
BspANI GGCC 2 cut(s) 151, 185
BspCNI CTCAG 1 cut(s) 496
BspEI TCCGGA 1 cut(s) 213
BspMAI CTGCAG 1 cut(s) 496
BspMI ACCTGC 1 cut(s) 63
BspQI GCTCTTC 1 cut(s) 275
BsrI ACTGG 1 cut(s) 65
BssMI GATC 1 cut(s) 418
Bst4CI ACNGT 2 cut(s) 37, 361
Bst6I CTCTTC 1 cut(s) 275
BstAPI GCANNNNNTGC 1 cut(s) 500
BstC8I GCNNGC 5 cut(s) 89, 158, 298, 349, 353
BstDEI CTNAG 1 cut(s) 504
BstHHI GCGC 2 cut(s) 11, 336
BstKTI GATC 1 cut(s) 421
BstMAI GTCTC 1 cut(s) 73
BstMBI GATC 1 cut(s) 418
BstMCI CGRYCG 1 cut(s) 421
BstMWI GCNNNNNNNGC 6 cut(s) 157, 274, 497, 500, 509, 518
BstNSI RCATGY 1 cut(s) 91
BstSFI CTRYAG 1 cut(s) 492
BstV1I GCAGC 4 cut(s) 478, 487, 499, 500
BstV2I GAAGAC 2 cut(s) 119, 159
BsuRI GGCC 2 cut(s) 151, 185
BveI ACCTGC 1 cut(s) 63
Cac8I GCNNGC 5 cut(s) 89, 158, 298, 349, 353
CaiI CAGNNNCTG 1 cut(s) 500
CfoI GCGC 2 cut(s) 11, 336
Cfr13I GGNCC 1 cut(s) 216
CviAII CATG 3 cut(s) 88, 274, 522
CviJI RGCY 9 cut(s) 23, 151, 185, 268, 296, 403, 491, 500, 512
CviKI_1 RGCY 9 cut(s) 23, 151, 185, 268, 296, 403, 491, 500, 512
DdeI CTNAG 1 cut(s) 504
DpnI GATC 1 cut(s) 420
DpnII GATC 1 cut(s) 418
EaeI YGGCCR 1 cut(s) 183
Eam1104I CTCTTC 1 cut(s) 275
EarI CTCTTC 1 cut(s) 275
Ecl136II GAGCTC 1 cut(s) 268
Eco24I GRGCYC 1 cut(s) 270
Eco47I GGWCC 1 cut(s) 216
Eco53kI GAGCTC 1 cut(s) 268
EcoICRI GAGCTC 1 cut(s) 268
EcoT38I GRGCYC 1 cut(s) 270
FaeI CATG 3 cut(s) 91, 277, 525
FaiI YATR 4 cut(s) 20, 89, 275, 523
FatI CATG 3 cut(s) 87, 273, 521
FblI GTMKAC 1 cut(s) 252
Fnu4HI GCNGC 4 cut(s) 489, 492, 501, 513
FriOI GRGCYC 1 cut(s) 270
Fsp4HI GCNGC 4 cut(s) 489, 492, 501, 513
FspI TGCGCA 1 cut(s) 335
GlaI GCGC 2 cut(s) 10, 335
GluI GCNGC 4 cut(s) 489, 492, 501, 513
GsuI CTGGAG 1 cut(s) 269
HaeIII GGCC 2 cut(s) 151, 185
HapII CCGG 1 cut(s) 214
HhaI GCGC 2 cut(s) 11, 336
Hin1II CATG 3 cut(s) 91, 277, 525
Hin6I GCGC 2 cut(s) 9, 334
HinP1I GCGC 2 cut(s) 9, 334
HincII GTYRAC 1 cut(s) 253
HindII GTYRAC 1 cut(s) 253
HinfI GANTC 2 cut(s) 93, 137
HpaII CCGG 1 cut(s) 214
Hpy166II GTNNAC 2 cut(s) 253, 309
Hpy188I TCNGA 2 cut(s) 132, 147
Hpy188III TCNNGA 3 cut(s) 214, 286, 408
Hpy8I GTNNAC 2 cut(s) 253, 309
Hpy99I CGWCG 5 cut(s) 64, 136, 166, 330, 463
HpyAV CCTTC 4 cut(s) 39, 88, 199, 502
HpyCH4III ACNGT 2 cut(s) 37, 361
HpyCH4V TGCA 4 cut(s) 475, 494, 515, 521
HpyF10VI GCNNNNNNNGC 6 cut(s) 157, 274, 497, 500, 509, 518
HpyF3I CTNAG 1 cut(s) 504
Hsp92II CATG 3 cut(s) 91, 277, 525
HspAI GCGC 2 cut(s) 9, 334
Kpn2I TCCGGA 1 cut(s) 213
Kzo9I GATC 1 cut(s) 418
LguI GCTCTTC 1 cut(s) 275
LmnI GCTCC 1 cut(s) 28
LpnPI CCDG 4 cut(s) 68, 78, 227, 299
Lsp1109I GCAGC 4 cut(s) 478, 487, 499, 500
MaeIII GTNAC 1 cut(s) 37
MalI GATC 1 cut(s) 420
MboI GATC 1 cut(s) 418
MboII GAAGA 8 cut(s) 119, 159, 209, 242, 262, 442, 478, 494
MhlI GDGCHC 2 cut(s) 158, 270
MlsI TGGCCA 1 cut(s) 185
MluCI AATT 1 cut(s) 390
MluNI TGGCCA 1 cut(s) 185
MlyI GAGTC 2 cut(s) 102, 146
MmeI TCCRAC 2 cut(s) 121, 155
Mox20I TGGCCA 1 cut(s) 185
MroI TCCGGA 1 cut(s) 213
MscI TGGCCA 1 cut(s) 185
MseI TTAA 1 cut(s) 363
MslI CAYNNNNRTG 1 cut(s) 520
Msp20I TGGCCA 1 cut(s) 185
MspA1I CMGCKG 1 cut(s) 491
MspI CCGG 1 cut(s) 214
Mva1269I GAATGC 1 cut(s) 336
MwoI GCNNNNNNNGC 6 cut(s) 157, 274, 497, 500, 509, 518
NdeII GATC 1 cut(s) 418
NlaIII CATG 3 cut(s) 91, 277, 525
NsbI TGCGCA 1 cut(s) 335
NspI RCATGY 1 cut(s) 91
PaeI GCATGC 1 cut(s) 91
PciSI GCTCTTC 1 cut(s) 275
PctI GAATGC 1 cut(s) 336
PflFI GACNNNGTC 1 cut(s) 136
PkrI GCNGC 4 cut(s) 490, 493, 502, 514
Ple19I CGATCG 1 cut(s) 421
PleI GAGTC 2 cut(s) 101, 145
PpsI GAGTC 2 cut(s) 101, 145
Psp124BI GAGCTC 1 cut(s) 270
PspPI GGNCC 1 cut(s) 216
PstI CTGCAG 1 cut(s) 496
PstNI CAGNNNCTG 1 cut(s) 500
PsyI GACNNNGTC 1 cut(s) 136
PvuI CGATCG 1 cut(s) 421
PvuII CAGCTG 1 cut(s) 491
RseI CAYNNNNRTG 1 cut(s) 520
SacI GAGCTC 1 cut(s) 270
SalI GTCGAC 1 cut(s) 251
SapI GCTCTTC 1 cut(s) 275
SaqAI TTAA 1 cut(s) 363
SatI GCNGC 4 cut(s) 489, 492, 501, 513
Sau3AI GATC 1 cut(s) 418
Sau96I GGNCC 1 cut(s) 216
SchI GAGTC 2 cut(s) 102, 146
SduI GDGCHC 2 cut(s) 158, 270
SetI ASST 8 cut(s) 25, 31, 57, 249, 258, 270, 493, 502
SfcI CTRYAG 1 cut(s) 492
SinI GGWCC 1 cut(s) 216
SmiMI CAYNNNNRTG 1 cut(s) 520
SphI GCATGC 1 cut(s) 91
Sse9I AATT 1 cut(s) 390
SsiI CCGC 1 cut(s) 452
SstI GAGCTC 1 cut(s) 270
TaaI ACNGT 2 cut(s) 37, 361
TaqI TCGA 4 cut(s) 252, 328, 417, 421
TasI AATT 1 cut(s) 390
Tru1I TTAA 1 cut(s) 363
Tru9I TTAA 1 cut(s) 363
TseI GCWGC 4 cut(s) 488, 491, 500, 512
TspDTI ATGAA 2 cut(s) 218, 262
TspGWI ACGGA 3 cut(s) 149, 314, 447
Tth111I GACNNNGTC 1 cut(s) 136
VpaK11BI GGWCC 1 cut(s) 216
XceI RCATGY 1 cut(s) 91
XmiI GTMKAC 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.