FvH4_1g20710

50S ribosomal protein L29

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0013530
Physical Location & Seq
Reverse (-)
12860575 .. 12863057
2483 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g20710.t1

Sequence Viewer

Length: 525 bp
ATGTTGGGCGCAATCTCCATAGCTCCGCCTTCCACGGTTGTACTACCCCAAAAGCTCCTCTCGCCCATCCCCAAGTCCTCCTTCACCGGCCTCAGACTCCAACACGTCTGCCCCGTCGCCATTCGCCTCCCCACGGCGTCGTTTCGGCGCTCCAGCTCGCCGTCCTCCTCGGTGGTGATGATGGCCAAGAGAGAGGAAGAGATGAAGGAAATCAGAACCAAGACGACCGAGGAAATCAACGAGGAGGTCGTCGACCTTAAAGGAGAGCTCTTCATGCTTCGTCTCCAGAAATCGGCCAGGAATGAGTTCACTTCCAGCGAGTTCCGGCGTATGCGCAAAAGGATTGCTCGCTTGCTCACTGTTAAGCGGGAAAGAGAGATCGAGGATGGAATTGGTAAGAGATTATCAAGAAAGTTTGACCGACAATGGAAGAGAAGCATTATTGTCAGACCACCTCCGTCCTTGAAGAAGTTGCAAGAGGAAGAAGCAGCTGCAGAAGCCGCCGAAGCTGCAAAATCTGCTTGA

Protein Analysis

175

Amino Acids

19.76

Weight (kDa)

10.38

Isoelectric Point (pI)

83.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L29 PF00831 69 - 125 3.2e-14 Ribosomal L29 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013530)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G65220
fragaria_vesca FvH4_1g20710
malus_domestica MD00G1199600.v1.1 MD01G1031300.v1.1
prunus_persica Prupe.6G188000_v2.0.a1
pyrus_communis pycom01g05890
rosa_chinensis RchiOBHm_Chr2g0113161
rosa_laevigata RLG00000018048
rosa_multiflora Rmu_sc0006838.1_g000024 Rmu_sc0035384.1_g000001
rosa_roxburghii Rroxscaffold_2G00129560 Rroxscaffold_2G00129750
rosa_rugosa Rorug02G0185900
rosa_samantha Rh2AG240200 Rh2BG254100 Rh2CG244200 Rh2DG248100
rosa_wichuraiana Rw2G018800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 335
AccI GTMKAC 1 cut(s) 252
AciI CCGC 3 cut(s) 26, 367, 501
AcoI YGGCCR 2 cut(s) 183, 294
AcyI GRCGYC 1 cut(s) 137
AfaI GTAC 1 cut(s) 42
AfiI CCNNNNNNNGG 2 cut(s) 133, 292
AflIII ACRYGT 1 cut(s) 103
AgsI TTSAA 1 cut(s) 466
AjiI CACGTC 1 cut(s) 106
AjnI CCWGG 1 cut(s) 296
AjuI GAANNNNNNNTTGG 2 cut(s) 65, 97
AluBI AGCT 6 cut(s) 23, 55, 156, 268, 491, 509
AluI AGCT 6 cut(s) 23, 55, 156, 268, 491, 509
Alw21I GWGCWC 1 cut(s) 270
Alw26I GTCTC 1 cut(s) 287
AoxI GGCC 3 cut(s) 88, 183, 294
ApeKI GCWGC 3 cut(s) 488, 491, 509
Asp700I GAANNNNTTC 1 cut(s) 305
AspLEI GCGC 3 cut(s) 11, 150, 336
AsuHPI GGTGA 2 cut(s) 76, 187
BalI TGGCCA 1 cut(s) 185
BanII GRGCYC 1 cut(s) 270
Bbv12I GWGCWC 1 cut(s) 270
BbvI GCAGC 3 cut(s) 478, 496, 500
BccI CCATC 3 cut(s) 74, 175, 380
BceAI ACGGC 2 cut(s) 145, 150
BciT130I CCWGG 1 cut(s) 298
BcoDI GTCTC 1 cut(s) 287
BfmI CTRYAG 1 cut(s) 492
BfoI RGCGCY 1 cut(s) 151
BisI GCNGC 4 cut(s) 489, 492, 501, 510
BlsI GCNGC 4 cut(s) 490, 493, 502, 511
Bme1390I CCNGG 1 cut(s) 298
BmgBI CACGTC 1 cut(s) 106
BmrFI CCNGG 1 cut(s) 298
BpmI CTGGAG 2 cut(s) 136, 269
BsaHI GRCGYC 1 cut(s) 137
BsaJI CCNNGG 4 cut(s) 33, 132, 168, 228
Bsc4I CCNNNNNNNGG 2 cut(s) 133, 292
Bse118I RCCGGY 1 cut(s) 86
BseBI CCWGG 1 cut(s) 298
BseDI CCNNGG 4 cut(s) 33, 132, 168, 228
BseGI GGATG 2 cut(s) 66, 391
BseLI CCNNNNNNNGG 2 cut(s) 133, 292
BseMII CTCAG 1 cut(s) 106
BseRI GAGGAG 3 cut(s) 47, 157, 257
BseXI GCAGC 3 cut(s) 478, 496, 500
Bsh1285I CGRYCG 1 cut(s) 228
BshFI GGCC 3 cut(s) 90, 185, 296
BsiEI CGRYCG 1 cut(s) 228
BsiHKAI GWGCWC 1 cut(s) 270
BsiSI CCGG 2 cut(s) 87, 325
BslI CCNNNNNNNGG 2 cut(s) 133, 292
BsmAI GTCTC 1 cut(s) 287
BsmBI CGTCTC 1 cut(s) 287
BsnI GGCC 3 cut(s) 90, 185, 296
Bsp1286I GDGCHC 1 cut(s) 270
Bsp143I GATC 1 cut(s) 378
BspACI CCGC 3 cut(s) 26, 367, 501
BspANI GGCC 3 cut(s) 90, 185, 296
BspCNI CTCAG 1 cut(s) 105
BspMAI CTGCAG 1 cut(s) 496
BspQI GCTCTTC 1 cut(s) 275
BsrFI RCCGGY 1 cut(s) 86
BssAI RCCGGY 1 cut(s) 86
BssECI CCNNGG 4 cut(s) 33, 132, 168, 228
BssMI GATC 1 cut(s) 378
BssNI GRCGYC 1 cut(s) 137
Bst2UI CCWGG 1 cut(s) 298
Bst4CI ACNGT 2 cut(s) 37, 361
Bst6I CTCTTC 3 cut(s) 192, 275, 425
BstACI GRCGYC 1 cut(s) 137
BstAPI GCANNNNNTGC 1 cut(s) 518
BstC8I GCNNGC 3 cut(s) 158, 349, 353
BstDEI CTNAG 1 cut(s) 92
BstDSI CCRYGG 2 cut(s) 33, 132
BstF5I GGATG 2 cut(s) 66, 391
BstH2I RGCGCY 1 cut(s) 151
BstHHI GCGC 3 cut(s) 11, 150, 336
BstKTI GATC 1 cut(s) 381
BstMAI GTCTC 1 cut(s) 287
BstMBI GATC 1 cut(s) 378
BstMCI CGRYCG 1 cut(s) 228
BstMWI GCNNNNNNNGC 7 cut(s) 61, 274, 497, 500, 506, 509, 518
BstNI CCWGG 1 cut(s) 298
BstSCI CCNGG 1 cut(s) 296
BstSFI CTRYAG 1 cut(s) 492
BstV1I GCAGC 3 cut(s) 478, 496, 500
BsuRI GGCC 3 cut(s) 90, 185, 296
BtgI CCRYGG 2 cut(s) 33, 132
BtrI CACGTC 1 cut(s) 106
BtsCI GGATG 2 cut(s) 66, 391
BtsIMutI CAGTG 1 cut(s) 357
Cac8I GCNNGC 3 cut(s) 158, 349, 353
CfoI GCGC 3 cut(s) 11, 150, 336
Cfr10I RCCGGY 1 cut(s) 86
CseI GACGC 1 cut(s) 126
Csp6I GTAC 1 cut(s) 41
CviAII CATG 1 cut(s) 274
CviQI GTAC 1 cut(s) 41
DdeI CTNAG 1 cut(s) 92
DpnI GATC 1 cut(s) 380
DpnII GATC 1 cut(s) 378
EaeI YGGCCR 2 cut(s) 183, 294
Eam1104I CTCTTC 3 cut(s) 192, 275, 425
EarI CTCTTC 3 cut(s) 192, 275, 425
EciI GGCGGA 1 cut(s) 15
Ecl136II GAGCTC 1 cut(s) 268
Eco24I GRGCYC 1 cut(s) 270
Eco53kI GAGCTC 1 cut(s) 268
EcoICRI GAGCTC 1 cut(s) 268
EcoRII CCWGG 1 cut(s) 296
EcoT38I GRGCYC 1 cut(s) 270
Esp3I CGTCTC 1 cut(s) 287
FaeI CATG 1 cut(s) 277
FaiI YATR 3 cut(s) 20, 275, 332
FalI AAGNNNNNCTT 2 cut(s) 65, 97
FatI CATG 1 cut(s) 273
FauI CCCGC 1 cut(s) 360
FblI GTMKAC 1 cut(s) 252
Fnu4HI GCNGC 4 cut(s) 489, 492, 501, 510
FokI GGATG 2 cut(s) 53, 398
FriOI GRGCYC 1 cut(s) 270
Fsp4HI GCNGC 4 cut(s) 489, 492, 501, 510
FspI TGCGCA 1 cut(s) 335
GlaI GCGC 3 cut(s) 10, 149, 335
GluI GCNGC 4 cut(s) 489, 492, 501, 510
GsuI CTGGAG 2 cut(s) 136, 269
HaeII RGCGCY 1 cut(s) 151
HaeIII GGCC 3 cut(s) 90, 185, 296
HapII CCGG 2 cut(s) 87, 325
HgaI GACGC 1 cut(s) 126
HhaI GCGC 3 cut(s) 11, 150, 336
Hin1I GRCGYC 1 cut(s) 137
Hin1II CATG 1 cut(s) 277
Hin6I GCGC 3 cut(s) 9, 148, 334
HinP1I GCGC 3 cut(s) 9, 148, 334
HincII GTYRAC 1 cut(s) 253
HindII GTYRAC 1 cut(s) 253
HinfI GANTC 1 cut(s) 96
HpaII CCGG 2 cut(s) 87, 325
HphI GGTGA 2 cut(s) 76, 187
Hpy166II GTNNAC 2 cut(s) 253, 309
Hpy188I TCNGA 3 cut(s) 95, 215, 449
Hpy188III TCNNGA 2 cut(s) 286, 408
Hpy8I GTNNAC 2 cut(s) 253, 309
Hpy99I CGWCG 3 cut(s) 119, 142, 254
HpyAV CCTTC 3 cut(s) 39, 91, 199
HpyCH4III ACNGT 2 cut(s) 37, 361
HpyCH4IV ACGT 1 cut(s) 105
HpyCH4V TGCA 3 cut(s) 475, 494, 512
HpyF10VI GCNNNNNNNGC 7 cut(s) 61, 274, 497, 500, 506, 509, 518
HpyF3I CTNAG 1 cut(s) 92
HpySE526I ACGT 1 cut(s) 105
Hsp92I GRCGYC 1 cut(s) 137
Hsp92II CATG 1 cut(s) 277
HspAI GCGC 3 cut(s) 9, 148, 334
Kzo9I GATC 1 cut(s) 378
LguI GCTCTTC 1 cut(s) 275
LmnI GCTCC 3 cut(s) 28, 60, 155
LpnPI CCDG 7 cut(s) 100, 166, 283, 299, 310, 328, 338
Lsp1109I GCAGC 3 cut(s) 478, 496, 500
MaeII ACGT 1 cut(s) 105
MalI GATC 1 cut(s) 380
MboI GATC 1 cut(s) 378
MboII GAAGA 5 cut(s) 209, 262, 442, 478, 494
MhlI GDGCHC 1 cut(s) 270
MlsI TGGCCA 1 cut(s) 185
MluCI AATT 1 cut(s) 390
MluNI TGGCCA 1 cut(s) 185
MlyI GAGTC 1 cut(s) 90
MmeI TCCRAC 1 cut(s) 124
Mox20I TGGCCA 1 cut(s) 185
MroXI GAANNNNTTC 1 cut(s) 305
MscI TGGCCA 1 cut(s) 185
MseI TTAA 2 cut(s) 258, 363
Msp20I TGGCCA 1 cut(s) 185
MspA1I CMGCKG 1 cut(s) 491
MspI CCGG 2 cut(s) 87, 325
MspR9I CCNGG 1 cut(s) 298
MvaI CCWGG 1 cut(s) 298
MwoI GCNNNNNNNGC 7 cut(s) 61, 274, 497, 500, 506, 509, 518
NdeII GATC 1 cut(s) 378
NlaIII CATG 1 cut(s) 277
NsbI TGCGCA 1 cut(s) 335
PciSI GCTCTTC 1 cut(s) 275
PcsI WCGNNNNNNNCGW 2 cut(s) 111, 246
PdmI GAANNNNTTC 1 cut(s) 305
PkrI GCNGC 4 cut(s) 490, 493, 502, 511
PleI GAGTC 1 cut(s) 90
PpsI GAGTC 1 cut(s) 90
Psp124BI GAGCTC 1 cut(s) 270
Psp6I CCWGG 1 cut(s) 296
PspGI CCWGG 1 cut(s) 296
PstI CTGCAG 1 cut(s) 496
PvuII CAGCTG 1 cut(s) 491
RsaI GTAC 1 cut(s) 42
RsaNI GTAC 1 cut(s) 41
SacI GAGCTC 1 cut(s) 270
SalI GTCGAC 1 cut(s) 251
SapI GCTCTTC 1 cut(s) 275
SaqAI TTAA 2 cut(s) 258, 363
SatI GCNGC 4 cut(s) 489, 492, 501, 510
Sau3AI GATC 1 cut(s) 378
SchI GAGTC 1 cut(s) 90
ScrFI CCNGG 1 cut(s) 298
SduI GDGCHC 1 cut(s) 270
SfcI CTRYAG 1 cut(s) 492
Sse9I AATT 1 cut(s) 390
SsiI CCGC 3 cut(s) 26, 367, 501
SstI GAGCTC 1 cut(s) 270
StyD4I CCNGG 1 cut(s) 296
TaaI ACNGT 2 cut(s) 37, 361
TaiI ACGT 1 cut(s) 108
TaqI TCGA 2 cut(s) 252, 381
TaqII GACCGA 2 cut(s) 242, 435
TasI AATT 1 cut(s) 390
TatI WGTACW 1 cut(s) 40
TauI GCSGC 1 cut(s) 503
Tru1I TTAA 2 cut(s) 258, 363
Tru9I TTAA 2 cut(s) 258, 363
TscAI CASTG 1 cut(s) 364
TseI GCWGC 3 cut(s) 488, 491, 509
TspDTI ATGAA 2 cut(s) 218, 262
TspGWI ACGGA 1 cut(s) 447
TspRI CASTG 1 cut(s) 364
XmiI GTMKAC 1 cut(s) 252
XmnI GAANNNNTTC 1 cut(s) 305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.