MD01G1031300.v1.1

50S ribosomal protein L29

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0013530
Physical Location & Seq
Forward (+)
11078200 .. 11079797
1598 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1031300.v1.1.491

Sequence Viewer

Length: 528 bp
ATGTTGGGCGCAATCTCCATAGCTCCACCTTCAACGGTTACTTTTCCCTCAAAGCTGCTGTCGCCAGTCTCCAAGTCCTCCTTCAATGGCATGCGAGTCCAACGCCTTTGCCCAAACATCGGCGTTCGTCTTCCGACGGAGTCATCTCGGAGGCCGTGCTCGCCGTCGTCCTCGGTTGTGATGATGGCCAAGAGAGAGGAAGAAATGAAGGAAATCAGGACCAAAACCACCGAAGAAATCAACGAGGAGGTGGTCGACCTCAAGGGAGAGCTCTTCATGCTTCGCCTCCAGAAATCGGCTCGCAACGAGTTCACTTCCAGCGAGTTCAGTCGAATGCGCAAAAGGATTGCTCGCTTGCTTACTGTTAAACGCGAAAGAGAGATTGAGGAGGGAATTGGTAAGAGGCTATCAAGAAAGTTCGATCGACAATGGAAGAAAAGCATTGTTGTTAGACCGCCTCCGTCATTGAAGAAGTTGCAAGAGGAAGAAGCAGCTGCAGAAGCTGCTGAGAAGGCTGCATCTGCATGA

Protein Analysis

176

Amino Acids

19.81

Weight (kDa)

10.1

Isoelectric Point (pI)

76.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L29 PF00831 70 - 126 7.8e-14 Ribosomal L29 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013530)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G65220
fragaria_vesca FvH4_1g20710
malus_domestica MD00G1199600.v1.1 MD01G1031300.v1.1
prunus_persica Prupe.6G188000_v2.0.a1
pyrus_communis pycom01g05890
rosa_chinensis RchiOBHm_Chr2g0113161
rosa_laevigata RLG00000018048
rosa_multiflora Rmu_sc0006838.1_g000024 Rmu_sc0035384.1_g000001
rosa_roxburghii Rroxscaffold_2G00129560 Rroxscaffold_2G00129750
rosa_rugosa Rorug02G0185900
rosa_samantha Rh2AG240200 Rh2BG254100 Rh2CG244200 Rh2DG248100
rosa_wichuraiana Rw2G018800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 338
AccI GTMKAC 1 cut(s) 255
AccII CGCG 1 cut(s) 372
AciI CCGC 1 cut(s) 455
AcoI YGGCCR 1 cut(s) 186
AfiI CCNNNNNNNGG 2 cut(s) 119, 295
AgsI TTSAA 3 cut(s) 33, 85, 469
AjuI GAANNNNNNNTTGG 2 cut(s) 65, 97
AluBI AGCT 5 cut(s) 23, 55, 271, 494, 503
AluI AGCT 5 cut(s) 23, 55, 271, 494, 503
Alw21I GWGCWC 2 cut(s) 161, 273
Alw26I GTCTC 1 cut(s) 73
AlwNI CAGNNNCTG 1 cut(s) 503
AoxI GGCC 2 cut(s) 152, 186
ApeKI GCWGC 5 cut(s) 55, 491, 494, 503, 515
ArsI GACNNNNNNTTYG 2 cut(s) 44, 76
AspLEI GCGC 2 cut(s) 11, 339
AspS9I GGNCC 1 cut(s) 219
AvaII GGWCC 1 cut(s) 219
BalI TGGCCA 1 cut(s) 188
BanII GRGCYC 1 cut(s) 273
BbsI GAAGAC 1 cut(s) 122
Bbv12I GWGCWC 2 cut(s) 161, 273
BbvI GCAGC 5 cut(s) 42, 481, 490, 502, 503
BccI CCATC 1 cut(s) 178
BceAI ACGGC 2 cut(s) 139, 148
BcoDI GTCTC 1 cut(s) 73
BfmI CTRYAG 1 cut(s) 495
BisI GCNGC 5 cut(s) 56, 492, 495, 504, 516
BlsI GCNGC 5 cut(s) 57, 493, 496, 505, 517
Bme18I GGWCC 1 cut(s) 219
BmgT120I GGNCC 1 cut(s) 219
BpiI GAAGAC 1 cut(s) 122
BpmI CTGGAG 1 cut(s) 272
BpuEI CTTGAG 1 cut(s) 245
BsaJI CCNNGG 1 cut(s) 171
Bsc4I CCNNNNNNNGG 2 cut(s) 119, 295
Bse1I ACTGG 1 cut(s) 65
BseDI CCNNGG 1 cut(s) 171
BseLI CCNNNNNNNGG 2 cut(s) 119, 295
BseMII CTCAG 1 cut(s) 498
BseNI ACTGG 1 cut(s) 65
BseRI GAGGAG 2 cut(s) 260, 401
BseXI GCAGC 5 cut(s) 42, 481, 490, 502, 503
Bsh1236I CGCG 1 cut(s) 372
Bsh1285I CGRYCG 1 cut(s) 424
BshFI GGCC 2 cut(s) 154, 188
BsiEI CGRYCG 1 cut(s) 424
BsiHKAI GWGCWC 2 cut(s) 161, 273
BslI CCNNNNNNNGG 2 cut(s) 119, 295
BsmAI GTCTC 1 cut(s) 73
BsmI GAATGC 1 cut(s) 339
BsnI GGCC 2 cut(s) 154, 188
Bsp1286I GDGCHC 2 cut(s) 161, 273
Bsp143I GATC 1 cut(s) 421
BspACI CCGC 1 cut(s) 455
BspANI GGCC 2 cut(s) 154, 188
BspCNI CTCAG 1 cut(s) 499
BspFNI CGCG 1 cut(s) 372
BspMAI CTGCAG 1 cut(s) 499
BspQI GCTCTTC 1 cut(s) 278
BsrI ACTGG 1 cut(s) 65
BssECI CCNNGG 1 cut(s) 171
BssMI GATC 1 cut(s) 421
Bst4CI ACNGT 2 cut(s) 37, 364
Bst6I CTCTTC 1 cut(s) 278
BstAPI GCANNNNNTGC 1 cut(s) 503
BstC8I GCNNGC 5 cut(s) 92, 161, 301, 352, 356
BstDEI CTNAG 1 cut(s) 507
BstFNI CGCG 1 cut(s) 372
BstHHI GCGC 2 cut(s) 11, 339
BstKTI GATC 1 cut(s) 424
BstMAI GTCTC 1 cut(s) 73
BstMBI GATC 1 cut(s) 421
BstMCI CGRYCG 1 cut(s) 424
BstMWI GCNNNNNNNGC 7 cut(s) 61, 160, 277, 500, 503, 512, 521
BstNSI RCATGY 1 cut(s) 94
BstSFI CTRYAG 1 cut(s) 495
BstUI CGCG 1 cut(s) 372
BstV1I GCAGC 5 cut(s) 42, 481, 490, 502, 503
BstV2I GAAGAC 1 cut(s) 122
BsuRI GGCC 2 cut(s) 154, 188
Cac8I GCNNGC 5 cut(s) 92, 161, 301, 352, 356
CaiI CAGNNNCTG 1 cut(s) 503
CfoI GCGC 2 cut(s) 11, 339
Cfr13I GGNCC 1 cut(s) 219
CviAII CATG 3 cut(s) 91, 277, 525
DdeI CTNAG 1 cut(s) 507
DpnI GATC 1 cut(s) 423
DpnII GATC 1 cut(s) 421
EaeI YGGCCR 1 cut(s) 186
Eam1104I CTCTTC 1 cut(s) 278
EarI CTCTTC 1 cut(s) 278
Ecl136II GAGCTC 1 cut(s) 271
Eco24I GRGCYC 1 cut(s) 273
Eco47I GGWCC 1 cut(s) 219
Eco53kI GAGCTC 1 cut(s) 271
EcoICRI GAGCTC 1 cut(s) 271
EcoT38I GRGCYC 1 cut(s) 273
FaeI CATG 3 cut(s) 94, 280, 528
FaiI YATR 4 cut(s) 20, 92, 278, 526
FalI AAGNNNNNCTT 2 cut(s) 65, 97
FatI CATG 3 cut(s) 90, 276, 524
FblI GTMKAC 1 cut(s) 255
Fnu4HI GCNGC 5 cut(s) 56, 492, 495, 504, 516
FriOI GRGCYC 1 cut(s) 273
Fsp4HI GCNGC 5 cut(s) 56, 492, 495, 504, 516
FspI TGCGCA 1 cut(s) 338
GlaI GCGC 2 cut(s) 10, 338
GluI GCNGC 5 cut(s) 56, 492, 495, 504, 516
GsuI CTGGAG 1 cut(s) 272
HaeIII GGCC 2 cut(s) 154, 188
HhaI GCGC 2 cut(s) 11, 339
Hin1II CATG 3 cut(s) 94, 280, 528
Hin6I GCGC 2 cut(s) 9, 337
HinP1I GCGC 2 cut(s) 9, 337
HincII GTYRAC 1 cut(s) 256
HindII GTYRAC 1 cut(s) 256
HinfI GANTC 2 cut(s) 96, 140
Hpy166II GTNNAC 2 cut(s) 256, 312
Hpy188I TCNGA 2 cut(s) 135, 150
Hpy188III TCNNGA 3 cut(s) 217, 289, 411
Hpy8I GTNNAC 2 cut(s) 256, 312
Hpy99I CGWCG 2 cut(s) 139, 169
HpyAV CCTTC 4 cut(s) 39, 91, 202, 505
HpyCH4III ACNGT 2 cut(s) 37, 364
HpyCH4V TGCA 4 cut(s) 478, 497, 518, 524
HpyF10VI GCNNNNNNNGC 7 cut(s) 61, 160, 277, 500, 503, 512, 521
HpyF3I CTNAG 1 cut(s) 507
Hsp92II CATG 3 cut(s) 94, 280, 528
HspAI GCGC 2 cut(s) 9, 337
Kzo9I GATC 1 cut(s) 421
LguI GCTCTTC 1 cut(s) 278
LmnI GCTCC 1 cut(s) 28
LpnPI CCDG 4 cut(s) 78, 202, 302, 331
Lsp1109I GCAGC 5 cut(s) 42, 481, 490, 502, 503
MaeIII GTNAC 1 cut(s) 37
MalI GATC 1 cut(s) 423
MboI GATC 1 cut(s) 421
MboII GAAGA 7 cut(s) 122, 212, 245, 265, 445, 481, 497
MhlI GDGCHC 2 cut(s) 161, 273
MlsI TGGCCA 1 cut(s) 188
MluCI AATT 1 cut(s) 393
MluNI TGGCCA 1 cut(s) 188
MlyI GAGTC 2 cut(s) 105, 149
MmeI TCCRAC 2 cut(s) 124, 158
Mox20I TGGCCA 1 cut(s) 188
MscI TGGCCA 1 cut(s) 188
MseI TTAA 1 cut(s) 366
MslI CAYNNNNRTG 1 cut(s) 523
Msp20I TGGCCA 1 cut(s) 188
MspA1I CMGCKG 1 cut(s) 494
Mva1269I GAATGC 1 cut(s) 339
MvnI CGCG 1 cut(s) 372
MwoI GCNNNNNNNGC 7 cut(s) 61, 160, 277, 500, 503, 512, 521
NdeII GATC 1 cut(s) 421
NlaIII CATG 3 cut(s) 94, 280, 528
NsbI TGCGCA 1 cut(s) 338
NspI RCATGY 1 cut(s) 94
PaeI GCATGC 1 cut(s) 94
PciSI GCTCTTC 1 cut(s) 278
PctI GAATGC 1 cut(s) 339
PflFI GACNNNGTC 1 cut(s) 139
PkrI GCNGC 5 cut(s) 57, 493, 496, 505, 517
Ple19I CGATCG 1 cut(s) 424
PleI GAGTC 2 cut(s) 104, 148
PpsI GAGTC 2 cut(s) 104, 148
Psp124BI GAGCTC 1 cut(s) 273
PspPI GGNCC 1 cut(s) 219
PstI CTGCAG 1 cut(s) 499
PstNI CAGNNNCTG 1 cut(s) 503
PsyI GACNNNGTC 1 cut(s) 139
PvuI CGATCG 1 cut(s) 424
PvuII CAGCTG 1 cut(s) 494
RseI CAYNNNNRTG 1 cut(s) 523
SacI GAGCTC 1 cut(s) 273
SalI GTCGAC 1 cut(s) 254
SapI GCTCTTC 1 cut(s) 278
SaqAI TTAA 1 cut(s) 366
SatI GCNGC 5 cut(s) 56, 492, 495, 504, 516
Sau3AI GATC 1 cut(s) 421
Sau96I GGNCC 1 cut(s) 219
SchI GAGTC 2 cut(s) 105, 149
SduI GDGCHC 2 cut(s) 161, 273
SetI ASST 8 cut(s) 25, 31, 57, 252, 261, 273, 496, 505
SfcI CTRYAG 1 cut(s) 495
SinI GGWCC 1 cut(s) 219
SmiMI CAYNNNNRTG 1 cut(s) 523
SmlI CTYRAG 1 cut(s) 260
SmoI CTYRAG 1 cut(s) 260
SphI GCATGC 1 cut(s) 94
Sse9I AATT 1 cut(s) 393
SsiI CCGC 1 cut(s) 455
SstI GAGCTC 1 cut(s) 273
TaaI ACNGT 2 cut(s) 37, 364
TaqI TCGA 4 cut(s) 255, 331, 420, 424
TasI AATT 1 cut(s) 393
Tru1I TTAA 1 cut(s) 366
Tru9I TTAA 1 cut(s) 366
TseI GCWGC 5 cut(s) 55, 491, 494, 503, 515
TspDTI ATGAA 2 cut(s) 221, 265
TspGWI ACGGA 2 cut(s) 152, 450
Tth111I GACNNNGTC 1 cut(s) 139
VpaK11BI GGWCC 1 cut(s) 219
XceI RCATGY 1 cut(s) 94
XmiI GTMKAC 1 cut(s) 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.