Rroxscaffold_2G00129560

50S ribosomal protein L29

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0013530
Physical Location & Seq
Forward (+)
65509325 .. 65511635
2311 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00129560.1

Sequence Viewer

Length: 534 bp
ATGACGACAATGTTGGGCGCAATCTCCATAGCTCCACCTTCCACGGTTGCCTTACCCCCAAAGCTCCTCTCTCCCGTCCCCAAGTCCTCCTTCAACGGCCTCAGACTCCAACACGTCTGCCCCGTCGGCATTCGACTCCCCACGGCGTCGTTTCGGAGGTCCAGCTCGCCGTCTTCCTCCGTGGTTATGATGGCCAAGAGAGAGGAAGAGATGAAGGAAATCAGAACCAAGACCACTGAAGAAATCAACGAGGAGGTGGTCGACCTTAAAGGAGAGCTCTTCATGCTTCGCCTCCAGAAATCGGCTCGGAATGAGTTCACATCCAGCGAGTTCCGGCGTATGCGCAAAAGGATTGCTCGCTTGCTTACTGTTAAACGGGAAAGAGAGATCGAGGATGGAATTGGTAAGAGGTTATCAAGAAAGTTCGACCGACAATGGAAGAGAAGCATCATCGTCAGACCACCTCCGTCCTTGAAGAAGTTGCAAGAGGAAGAAGCAGCTGCAGAAGCCGCAGAAGCTGCAAAATCTGCTTGA

Protein Analysis

177

Amino Acids

20.02

Weight (kDa)

10.38

Isoelectric Point (pI)

82.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L29 PF00831 72 - 128 3.3e-14 Ribosomal L29 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013530)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G65220
fragaria_vesca FvH4_1g20710
malus_domestica MD00G1199600.v1.1 MD01G1031300.v1.1
prunus_persica Prupe.6G188000_v2.0.a1
pyrus_communis pycom01g05890
rosa_chinensis RchiOBHm_Chr2g0113161
rosa_laevigata RLG00000018048
rosa_multiflora Rmu_sc0006838.1_g000024 Rmu_sc0035384.1_g000001
rosa_roxburghii Rroxscaffold_2G00129560 Rroxscaffold_2G00129750
rosa_rugosa Rorug02G0185900
rosa_samantha Rh2AG240200 Rh2BG254100 Rh2CG244200 Rh2DG248100
rosa_wichuraiana Rw2G018800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 344
AccI GTMKAC 1 cut(s) 261
AciI CCGC 1 cut(s) 510
AcoI YGGCCR 1 cut(s) 192
AcuI CTGAAG 1 cut(s) 258
AcyI GRCGYC 1 cut(s) 146
AfiI CCNNNNNNNGG 1 cut(s) 301
AflIII ACRYGT 1 cut(s) 112
AgsI TTSAA 2 cut(s) 94, 475
AjiI CACGTC 1 cut(s) 115
AjuI GAANNNNNNNTTGG 2 cut(s) 74, 106
AluBI AGCT 6 cut(s) 32, 64, 165, 277, 500, 518
AluI AGCT 6 cut(s) 32, 64, 165, 277, 500, 518
Alw21I GWGCWC 1 cut(s) 279
AlwNI CAGNNNCTG 1 cut(s) 518
AoxI GGCC 2 cut(s) 97, 192
ApeKI GCWGC 3 cut(s) 497, 500, 518
Asp700I GAANNNNTTC 1 cut(s) 314
AspLEI GCGC 2 cut(s) 20, 345
AspS9I GGNCC 1 cut(s) 159
AvaII GGWCC 1 cut(s) 159
BalI TGGCCA 1 cut(s) 194
BanII GRGCYC 1 cut(s) 279
BbsI GAAGAC 1 cut(s) 165
Bbv12I GWGCWC 1 cut(s) 279
BbvI GCAGC 3 cut(s) 487, 505, 509
BccI CCATC 2 cut(s) 184, 389
BceAI ACGGC 3 cut(s) 112, 154, 159
BfmI CTRYAG 1 cut(s) 501
BglI GCCNNNNNGGC 1 cut(s) 126
BisI GCNGC 4 cut(s) 498, 501, 510, 519
BlsI GCNGC 4 cut(s) 499, 502, 511, 520
Bme18I GGWCC 1 cut(s) 159
BmgBI CACGTC 1 cut(s) 115
BmgT120I GGNCC 1 cut(s) 159
BmsI GCATC 1 cut(s) 456
BpiI GAAGAC 1 cut(s) 165
BpmI CTGGAG 1 cut(s) 278
BsaHI GRCGYC 1 cut(s) 146
BsaJI CCNNGG 3 cut(s) 42, 141, 180
Bsc4I CCNNNNNNNGG 1 cut(s) 301
BseDI CCNNGG 3 cut(s) 42, 141, 180
BseGI GGATG 2 cut(s) 320, 400
BseLI CCNNNNNNNGG 1 cut(s) 301
BseMII CTCAG 1 cut(s) 115
BseRI GAGGAG 2 cut(s) 56, 266
BseXI GCAGC 3 cut(s) 487, 505, 509
Bsh1285I CGRYCG 1 cut(s) 430
BshFI GGCC 2 cut(s) 99, 194
BsiEI CGRYCG 1 cut(s) 430
BsiHKAI GWGCWC 1 cut(s) 279
BsiSI CCGG 1 cut(s) 334
BslFI GGGAC 1 cut(s) 62
BslI CCNNNNNNNGG 1 cut(s) 301
BsmFI GGGAC 1 cut(s) 62
BsmI GAATGC 1 cut(s) 129
BsnI GGCC 2 cut(s) 99, 194
Bsp1286I GDGCHC 1 cut(s) 279
Bsp143I GATC 1 cut(s) 387
BspACI CCGC 1 cut(s) 510
BspANI GGCC 2 cut(s) 99, 194
BspCNI CTCAG 1 cut(s) 114
BspMAI CTGCAG 1 cut(s) 505
BspQI GCTCTTC 1 cut(s) 284
BssECI CCNNGG 3 cut(s) 42, 141, 180
BssMI GATC 1 cut(s) 387
BssNI GRCGYC 1 cut(s) 146
Bst4CI ACNGT 2 cut(s) 46, 370
Bst6I CTCTTC 3 cut(s) 201, 284, 434
BstACI GRCGYC 1 cut(s) 146
BstAPI GCANNNNNTGC 2 cut(s) 518, 527
BstC8I GCNNGC 3 cut(s) 167, 358, 362
BstDEI CTNAG 1 cut(s) 101
BstDSI CCRYGG 3 cut(s) 42, 141, 180
BstF5I GGATG 2 cut(s) 320, 400
BstHHI GCGC 2 cut(s) 20, 345
BstKTI GATC 1 cut(s) 390
BstMBI GATC 1 cut(s) 387
BstMCI CGRYCG 1 cut(s) 430
BstMWI GCNNNNNNNGC 7 cut(s) 126, 283, 506, 509, 515, 518, 527
BstSFI CTRYAG 1 cut(s) 501
BstV1I GCAGC 3 cut(s) 487, 505, 509
BstV2I GAAGAC 1 cut(s) 165
BsuRI GGCC 2 cut(s) 99, 194
BtgI CCRYGG 3 cut(s) 42, 141, 180
BtrI CACGTC 1 cut(s) 115
BtsCI GGATG 2 cut(s) 320, 400
BtsIMutI CAGTG 1 cut(s) 234
Cac8I GCNNGC 3 cut(s) 167, 358, 362
CaiI CAGNNNCTG 1 cut(s) 518
CfoI GCGC 2 cut(s) 20, 345
Cfr13I GGNCC 1 cut(s) 159
CseI GACGC 1 cut(s) 135
CviAII CATG 1 cut(s) 283
DdeI CTNAG 1 cut(s) 101
DpnI GATC 1 cut(s) 389
DpnII GATC 1 cut(s) 387
EaeI YGGCCR 1 cut(s) 192
Eam1104I CTCTTC 3 cut(s) 201, 284, 434
EarI CTCTTC 3 cut(s) 201, 284, 434
Ecl136II GAGCTC 1 cut(s) 277
Eco24I GRGCYC 1 cut(s) 279
Eco47I GGWCC 1 cut(s) 159
Eco53kI GAGCTC 1 cut(s) 277
Eco57I CTGAAG 1 cut(s) 258
EcoICRI GAGCTC 1 cut(s) 277
EcoT38I GRGCYC 1 cut(s) 279
FaeI CATG 1 cut(s) 286
FaiI YATR 4 cut(s) 29, 188, 284, 341
FalI AAGNNNNNCTT 2 cut(s) 74, 106
FaqI GGGAC 1 cut(s) 62
FatI CATG 1 cut(s) 282
FblI GTMKAC 1 cut(s) 261
Fnu4HI GCNGC 4 cut(s) 498, 501, 510, 519
FokI GGATG 2 cut(s) 307, 407
FriOI GRGCYC 1 cut(s) 279
Fsp4HI GCNGC 4 cut(s) 498, 501, 510, 519
FspI TGCGCA 1 cut(s) 344
GlaI GCGC 2 cut(s) 19, 344
GluI GCNGC 4 cut(s) 498, 501, 510, 519
GsuI CTGGAG 1 cut(s) 278
HaeIII GGCC 2 cut(s) 99, 194
HapII CCGG 1 cut(s) 334
HgaI GACGC 1 cut(s) 135
HhaI GCGC 2 cut(s) 20, 345
Hin1I GRCGYC 1 cut(s) 146
Hin1II CATG 1 cut(s) 286
Hin6I GCGC 2 cut(s) 18, 343
HinP1I GCGC 2 cut(s) 18, 343
HincII GTYRAC 1 cut(s) 262
HindII GTYRAC 1 cut(s) 262
HinfI GANTC 2 cut(s) 105, 135
HpaII CCGG 1 cut(s) 334
Hpy166II GTNNAC 2 cut(s) 262, 318
Hpy188I TCNGA 5 cut(s) 104, 156, 224, 309, 458
Hpy188III TCNNGA 2 cut(s) 295, 417
Hpy8I GTNNAC 2 cut(s) 262, 318
Hpy99I CGWCG 2 cut(s) 128, 151
HpyAV CCTTC 3 cut(s) 48, 100, 208
HpyCH4III ACNGT 2 cut(s) 46, 370
HpyCH4IV ACGT 1 cut(s) 114
HpyCH4V TGCA 3 cut(s) 484, 503, 521
HpyF10VI GCNNNNNNNGC 7 cut(s) 126, 283, 506, 509, 515, 518, 527
HpyF3I CTNAG 1 cut(s) 101
HpySE526I ACGT 1 cut(s) 114
Hsp92I GRCGYC 1 cut(s) 146
Hsp92II CATG 1 cut(s) 286
HspAI GCGC 2 cut(s) 18, 343
Kzo9I GATC 1 cut(s) 387
LguI GCTCTTC 1 cut(s) 284
LmnI GCTCC 2 cut(s) 37, 69
LpnPI CCDG 4 cut(s) 175, 308, 337, 347
Lsp1109I GCAGC 3 cut(s) 487, 505, 509
LweI GCATC 1 cut(s) 456
MaeII ACGT 1 cut(s) 114
MalI GATC 1 cut(s) 389
MboI GATC 1 cut(s) 387
MboII GAAGA 7 cut(s) 165, 218, 251, 271, 451, 487, 503
MhlI GDGCHC 1 cut(s) 279
MlsI TGGCCA 1 cut(s) 194
MluCI AATT 1 cut(s) 399
MluNI TGGCCA 1 cut(s) 194
MlyI GAGTC 2 cut(s) 99, 129
MmeI TCCRAC 1 cut(s) 133
Mox20I TGGCCA 1 cut(s) 194
MroXI GAANNNNTTC 1 cut(s) 314
MscI TGGCCA 1 cut(s) 194
MseI TTAA 2 cut(s) 267, 372
Msp20I TGGCCA 1 cut(s) 194
MspA1I CMGCKG 1 cut(s) 500
MspI CCGG 1 cut(s) 334
Mva1269I GAATGC 1 cut(s) 129
MwoI GCNNNNNNNGC 7 cut(s) 126, 283, 506, 509, 515, 518, 527
NdeII GATC 1 cut(s) 387
NlaIII CATG 1 cut(s) 286
NsbI TGCGCA 1 cut(s) 344
PciSI GCTCTTC 1 cut(s) 284
PcsI WCGNNNNNNNCGW 1 cut(s) 120
PctI GAATGC 1 cut(s) 129
PdmI GAANNNNTTC 1 cut(s) 314
PkrI GCNGC 4 cut(s) 499, 502, 511, 520
PleI GAGTC 2 cut(s) 99, 129
PpsI GAGTC 2 cut(s) 99, 129
Psp124BI GAGCTC 1 cut(s) 279
PspPI GGNCC 1 cut(s) 159
PstI CTGCAG 1 cut(s) 505
PstNI CAGNNNCTG 1 cut(s) 518
PvuII CAGCTG 1 cut(s) 500
SacI GAGCTC 1 cut(s) 279
SalI GTCGAC 1 cut(s) 260
SapI GCTCTTC 1 cut(s) 284
SaqAI TTAA 2 cut(s) 267, 372
SatI GCNGC 4 cut(s) 498, 501, 510, 519
Sau3AI GATC 1 cut(s) 387
Sau96I GGNCC 1 cut(s) 159
SchI GAGTC 2 cut(s) 99, 129
SduI GDGCHC 1 cut(s) 279
SfaNI GCATC 1 cut(s) 456
SfcI CTRYAG 1 cut(s) 501
SinI GGWCC 1 cut(s) 159
Sse9I AATT 1 cut(s) 399
SsiI CCGC 1 cut(s) 510
SstI GAGCTC 1 cut(s) 279
TaaI ACNGT 2 cut(s) 46, 370
TaiI ACGT 1 cut(s) 117
TaqI TCGA 4 cut(s) 133, 261, 390, 426
TaqII GACCGA 1 cut(s) 444
TasI AATT 1 cut(s) 399
TauI GCSGC 1 cut(s) 512
Tru1I TTAA 2 cut(s) 267, 372
Tru9I TTAA 2 cut(s) 267, 372
TscAI CASTG 1 cut(s) 241
TseI GCWGC 3 cut(s) 497, 500, 518
TspDTI ATGAA 2 cut(s) 227, 271
TspGWI ACGGA 2 cut(s) 169, 456
TspRI CASTG 1 cut(s) 241
VpaK11BI GGWCC 1 cut(s) 159
XmiI GTMKAC 1 cut(s) 261
XmnI GAANNNNTTC 1 cut(s) 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.