Rroxscaffold_2G00129750

50S ribosomal protein L29

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0013530
Physical Location & Seq
Reverse (-)
65777487 .. 65779693
2207 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00129750.1

Sequence Viewer

Length: 525 bp
ATGTTGGGCGCAATCTCCATAGCTCCACCTTCCACGGTTGCCTTACCCCCAAAGCTCCTCTCTCCCGTCCCCAAGTCCTCCTTCAACGGCCTCAGACTCCAACACGTCTGCCCCGTCGGCATTCGCCTCCCCACGGCGTCGTTTCGGAGGTCCAGCTCGCCGTCTTCCTCCGTGGTTATGATGGCCAAGAGAGAGGAAGAGATGAAGGAAATCAGAACCAAGACCACTGAAGAAATCAACGAGGAGGTGGTCGACCTTAAAGGAGAGCTCTTCATGCTTCGCCTCCAGAAATCGGCTCGGAATGAGTTCACATCCAGCGAGTTCCGTCGGATGCGCAAAAGGATTGCTCGCTTGCTTACTGTTAAACGGGAAAGAGAGATCGAGGATGGAATTGGTAAGAGGTTATCAAGAAAGTTCGACCGACAATGGAAGAGAAGCATCATCGTCAGACCACCTCCGTCCTTGAAGAAGTTGCAAGAGGAAGAAGCAGCTGCGGAAGCCGCAGAAGCTGCAAAATCTGCTTGA

Protein Analysis

174

Amino Acids

19.68

Weight (kDa)

10.38

Isoelectric Point (pI)

84.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L29 PF00831 69 - 125 3.2e-14 Ribosomal L29 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013530)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G65220
fragaria_vesca FvH4_1g20710
malus_domestica MD00G1199600.v1.1 MD01G1031300.v1.1
prunus_persica Prupe.6G188000_v2.0.a1
pyrus_communis pycom01g05890
rosa_chinensis RchiOBHm_Chr2g0113161
rosa_laevigata RLG00000018048
rosa_multiflora Rmu_sc0006838.1_g000024 Rmu_sc0035384.1_g000001
rosa_roxburghii Rroxscaffold_2G00129560 Rroxscaffold_2G00129750
rosa_rugosa Rorug02G0185900
rosa_samantha Rh2AG240200 Rh2BG254100 Rh2CG244200 Rh2DG248100
rosa_wichuraiana Rw2G018800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 335
AccI GTMKAC 1 cut(s) 252
AciI CCGC 2 cut(s) 494, 501
AcoI YGGCCR 1 cut(s) 183
AcuI CTGAAG 1 cut(s) 249
AcyI GRCGYC 1 cut(s) 137
AfiI CCNNNNNNNGG 2 cut(s) 133, 292
AflIII ACRYGT 1 cut(s) 103
AgsI TTSAA 2 cut(s) 85, 466
AjiI CACGTC 1 cut(s) 106
AjuI GAANNNNNNNTTGG 2 cut(s) 65, 97
AluBI AGCT 6 cut(s) 23, 55, 156, 268, 491, 509
AluI AGCT 6 cut(s) 23, 55, 156, 268, 491, 509
Alw21I GWGCWC 1 cut(s) 270
AlwNI CAGNNNCTG 1 cut(s) 509
AoxI GGCC 2 cut(s) 88, 183
ApeKI GCWGC 3 cut(s) 488, 491, 509
Asp700I GAANNNNTTC 1 cut(s) 305
AspLEI GCGC 2 cut(s) 11, 336
AspS9I GGNCC 1 cut(s) 150
AvaII GGWCC 1 cut(s) 150
BalI TGGCCA 1 cut(s) 185
BanII GRGCYC 1 cut(s) 270
BbsI GAAGAC 1 cut(s) 156
Bbv12I GWGCWC 1 cut(s) 270
BbvI GCAGC 3 cut(s) 478, 496, 500
BccI CCATC 2 cut(s) 175, 380
BceAI ACGGC 3 cut(s) 103, 145, 150
BglI GCCNNNNNGGC 1 cut(s) 117
BisI GCNGC 4 cut(s) 489, 492, 501, 510
BlsI GCNGC 4 cut(s) 490, 493, 502, 511
Bme18I GGWCC 1 cut(s) 150
BmgBI CACGTC 1 cut(s) 106
BmgT120I GGNCC 1 cut(s) 150
BmsI GCATC 2 cut(s) 321, 447
BpiI GAAGAC 1 cut(s) 156
BpmI CTGGAG 1 cut(s) 269
BsaHI GRCGYC 1 cut(s) 137
BsaJI CCNNGG 3 cut(s) 33, 132, 171
Bsc4I CCNNNNNNNGG 2 cut(s) 133, 292
BseDI CCNNGG 3 cut(s) 33, 132, 171
BseGI GGATG 3 cut(s) 311, 336, 391
BseLI CCNNNNNNNGG 2 cut(s) 133, 292
BseMII CTCAG 1 cut(s) 106
BseRI GAGGAG 2 cut(s) 47, 257
BseXI GCAGC 3 cut(s) 478, 496, 500
Bsh1285I CGRYCG 1 cut(s) 421
BshFI GGCC 2 cut(s) 90, 185
BsiEI CGRYCG 1 cut(s) 421
BsiHKAI GWGCWC 1 cut(s) 270
BslFI GGGAC 1 cut(s) 53
BslI CCNNNNNNNGG 2 cut(s) 133, 292
BsmFI GGGAC 1 cut(s) 53
BsmI GAATGC 1 cut(s) 120
BsnI GGCC 2 cut(s) 90, 185
Bsp1286I GDGCHC 1 cut(s) 270
Bsp143I GATC 1 cut(s) 378
BspACI CCGC 2 cut(s) 494, 501
BspANI GGCC 2 cut(s) 90, 185
BspCNI CTCAG 1 cut(s) 105
BspQI GCTCTTC 1 cut(s) 275
BssECI CCNNGG 3 cut(s) 33, 132, 171
BssMI GATC 1 cut(s) 378
BssNI GRCGYC 1 cut(s) 137
Bst4CI ACNGT 2 cut(s) 37, 361
Bst6I CTCTTC 3 cut(s) 192, 275, 425
BstACI GRCGYC 1 cut(s) 137
BstAPI GCANNNNNTGC 2 cut(s) 509, 518
BstC8I GCNNGC 3 cut(s) 158, 349, 353
BstDEI CTNAG 1 cut(s) 92
BstDSI CCRYGG 3 cut(s) 33, 132, 171
BstF5I GGATG 3 cut(s) 311, 336, 391
BstHHI GCGC 2 cut(s) 11, 336
BstKTI GATC 1 cut(s) 381
BstMBI GATC 1 cut(s) 378
BstMCI CGRYCG 1 cut(s) 421
BstMWI GCNNNNNNNGC 7 cut(s) 117, 274, 497, 500, 506, 509, 518
BstV1I GCAGC 3 cut(s) 478, 496, 500
BstV2I GAAGAC 1 cut(s) 156
BsuRI GGCC 2 cut(s) 90, 185
BtgI CCRYGG 3 cut(s) 33, 132, 171
BtrI CACGTC 1 cut(s) 106
BtsCI GGATG 3 cut(s) 311, 336, 391
BtsIMutI CAGTG 1 cut(s) 225
Cac8I GCNNGC 3 cut(s) 158, 349, 353
CaiI CAGNNNCTG 1 cut(s) 509
CfoI GCGC 2 cut(s) 11, 336
Cfr13I GGNCC 1 cut(s) 150
CseI GACGC 1 cut(s) 126
CviAII CATG 1 cut(s) 274
DdeI CTNAG 1 cut(s) 92
DpnI GATC 1 cut(s) 380
DpnII GATC 1 cut(s) 378
EaeI YGGCCR 1 cut(s) 183
Eam1104I CTCTTC 3 cut(s) 192, 275, 425
EarI CTCTTC 3 cut(s) 192, 275, 425
Ecl136II GAGCTC 1 cut(s) 268
Eco24I GRGCYC 1 cut(s) 270
Eco47I GGWCC 1 cut(s) 150
Eco53kI GAGCTC 1 cut(s) 268
Eco57I CTGAAG 1 cut(s) 249
EcoICRI GAGCTC 1 cut(s) 268
EcoT38I GRGCYC 1 cut(s) 270
FaeI CATG 1 cut(s) 277
FaiI YATR 3 cut(s) 20, 179, 275
FalI AAGNNNNNCTT 2 cut(s) 65, 97
FaqI GGGAC 1 cut(s) 53
FatI CATG 1 cut(s) 273
FblI GTMKAC 1 cut(s) 252
Fnu4HI GCNGC 4 cut(s) 489, 492, 501, 510
FokI GGATG 3 cut(s) 298, 343, 398
FriOI GRGCYC 1 cut(s) 270
Fsp4HI GCNGC 4 cut(s) 489, 492, 501, 510
FspI TGCGCA 1 cut(s) 335
GlaI GCGC 2 cut(s) 10, 335
GluI GCNGC 4 cut(s) 489, 492, 501, 510
GsuI CTGGAG 1 cut(s) 269
HaeIII GGCC 2 cut(s) 90, 185
HgaI GACGC 1 cut(s) 126
HhaI GCGC 2 cut(s) 11, 336
Hin1I GRCGYC 1 cut(s) 137
Hin1II CATG 1 cut(s) 277
Hin6I GCGC 2 cut(s) 9, 334
HinP1I GCGC 2 cut(s) 9, 334
HincII GTYRAC 1 cut(s) 253
HindII GTYRAC 1 cut(s) 253
HinfI GANTC 1 cut(s) 96
Hpy166II GTNNAC 2 cut(s) 253, 309
Hpy188I TCNGA 6 cut(s) 95, 147, 215, 300, 330, 449
Hpy188III TCNNGA 2 cut(s) 286, 408
Hpy8I GTNNAC 2 cut(s) 253, 309
Hpy99I CGWCG 3 cut(s) 119, 142, 330
HpyAV CCTTC 3 cut(s) 39, 91, 199
HpyCH4III ACNGT 2 cut(s) 37, 361
HpyCH4IV ACGT 1 cut(s) 105
HpyCH4V TGCA 2 cut(s) 475, 512
HpyF10VI GCNNNNNNNGC 7 cut(s) 117, 274, 497, 500, 506, 509, 518
HpyF3I CTNAG 1 cut(s) 92
HpySE526I ACGT 1 cut(s) 105
Hsp92I GRCGYC 1 cut(s) 137
Hsp92II CATG 1 cut(s) 277
HspAI GCGC 2 cut(s) 9, 334
Kzo9I GATC 1 cut(s) 378
LguI GCTCTTC 1 cut(s) 275
LmnI GCTCC 2 cut(s) 28, 60
LpnPI CCDG 3 cut(s) 166, 299, 328
Lsp1109I GCAGC 3 cut(s) 478, 496, 500
LweI GCATC 2 cut(s) 321, 447
MaeII ACGT 1 cut(s) 105
MalI GATC 1 cut(s) 380
MboI GATC 1 cut(s) 378
MboII GAAGA 7 cut(s) 156, 209, 242, 262, 442, 478, 494
MhlI GDGCHC 1 cut(s) 270
MlsI TGGCCA 1 cut(s) 185
MluCI AATT 1 cut(s) 390
MluNI TGGCCA 1 cut(s) 185
MlyI GAGTC 1 cut(s) 90
MmeI TCCRAC 2 cut(s) 124, 308
Mox20I TGGCCA 1 cut(s) 185
MroXI GAANNNNTTC 1 cut(s) 305
MscI TGGCCA 1 cut(s) 185
MseI TTAA 2 cut(s) 258, 363
Msp20I TGGCCA 1 cut(s) 185
MspA1I CMGCKG 1 cut(s) 491
Mva1269I GAATGC 1 cut(s) 120
MwoI GCNNNNNNNGC 7 cut(s) 117, 274, 497, 500, 506, 509, 518
NdeII GATC 1 cut(s) 378
NlaIII CATG 1 cut(s) 277
NsbI TGCGCA 1 cut(s) 335
PciSI GCTCTTC 1 cut(s) 275
PcsI WCGNNNNNNNCGW 1 cut(s) 111
PctI GAATGC 1 cut(s) 120
PdmI GAANNNNTTC 1 cut(s) 305
PkrI GCNGC 4 cut(s) 490, 493, 502, 511
PleI GAGTC 1 cut(s) 90
PpsI GAGTC 1 cut(s) 90
Psp124BI GAGCTC 1 cut(s) 270
PspPI GGNCC 1 cut(s) 150
PstNI CAGNNNCTG 1 cut(s) 509
PvuII CAGCTG 1 cut(s) 491
SacI GAGCTC 1 cut(s) 270
SalI GTCGAC 1 cut(s) 251
SapI GCTCTTC 1 cut(s) 275
SaqAI TTAA 2 cut(s) 258, 363
SatI GCNGC 4 cut(s) 489, 492, 501, 510
Sau3AI GATC 1 cut(s) 378
Sau96I GGNCC 1 cut(s) 150
SchI GAGTC 1 cut(s) 90
SduI GDGCHC 1 cut(s) 270
SfaNI GCATC 2 cut(s) 321, 447
SinI GGWCC 1 cut(s) 150
Sse9I AATT 1 cut(s) 390
SsiI CCGC 2 cut(s) 494, 501
SstI GAGCTC 1 cut(s) 270
TaaI ACNGT 2 cut(s) 37, 361
TaiI ACGT 1 cut(s) 108
TaqI TCGA 3 cut(s) 252, 381, 417
TaqII GACCGA 1 cut(s) 435
TasI AATT 1 cut(s) 390
TauI GCSGC 1 cut(s) 503
Tru1I TTAA 2 cut(s) 258, 363
Tru9I TTAA 2 cut(s) 258, 363
TscAI CASTG 1 cut(s) 232
TseI GCWGC 3 cut(s) 488, 491, 509
TspDTI ATGAA 2 cut(s) 218, 262
TspGWI ACGGA 3 cut(s) 160, 314, 447
TspRI CASTG 1 cut(s) 232
VpaK11BI GGWCC 1 cut(s) 150
XmiI GTMKAC 1 cut(s) 252
XmnI GAANNNNTTC 1 cut(s) 305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.