Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0003296

Physical Location & Seq

Genomic Coordinates Reverse (-)

26485666 .. 26491721

Gene Structure 6056 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G66310.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3192 bp

ATGACTACAGAAGATGATGATCAGATGCTAGGACCAAGCGGCACCCAGGAAAAGATCTATGTTTCTGTTCGTATGCGACCTTTAAACGACAAGGAAAAGTTTAGGAATGATGTCCCTGACTGGGAGTGCATCAACAATACTACCATCATTTACAGGAGTCATCTTTCTATCTCCGAGCGTTCCATGTATCCTTCTGCTTACACATTTGACAGAGTGTTTAGCCCTGAGTGTTGTACAAGGCAGGTGTATGAACAAGGGGCAAAGGAAGTCGCTTTCTCTGTTGTCAGTGGCGTTAATGCTAGTGTATTCGCATATGGGCAAACAAGCAGTGGAAAGACGTACACTATGAGCGGAATTACTGACTGCGCCTTGGTTGATATATATGGCTACATTGACAAGCACAAGGAAAGAGAATTCATTCTTAAGTTCTCTGCTATGGAGATCTACAATGAGTCTGTAAGAGATCTCTTAAGTACAGATACTAGTCCACTCAGACTTTTAGATGACCCTGAGAAAGGGACAGTTGTTGAGAAACTCACAGAGGAAACTCTGCGAGACTGGAATCATTTCAAGGAGCTCCTATCTGTCTGCAAAGCACAAAGGCAAATTGGTGAGACAGCTCTAAATGAAGTTAGCTCCCGGTCTCATCAGATTTTGAGATTGACAGTAGAAAGCATAGCTCGTGAATTTTCTACCAATGATAAGTTTAGTACTCTAACAGCGACAGTGAACTTCATTGATCTTGCTGGAAGTGAACGTGCGTCTCAATCATTATCAGCCGGCACAAGGTTAAAAGAAGGTTGTCATATAAACCGAAGTTTGTTAACACTGGGAACTGTGATCCGTAAGCTAAGTAAAGAAAAAACTGGGCACATTCCATTCAGAGATTCAAAACTGACACGCATACTTCAGTCTTCTTTGGGAGGAAATGCAAGAACTGCCATAATTTGCACTATGAGTCCTGCGAGAATCCATGTTGAACAATCAAGAAACACACTGTTATTTGCAAGCTGCGCAAAGGAGGTGACAACAAATGCACAAGTCAATGTTGTCATGTCTGATAAAGCTTTAGTAAAGCACTTGCAGAGGGAGTTGGCTAAACTGGAAAGTGAGTTGAGAAGCCCTAGTCAGGCTTCAATTGTTTCTGACACAACTGCATTACTGACGGAGAAAGACCTTGAGGTTGAAAAGCTTAAGAAAGAGGTATTCCAACTAGCACAACAACTAGAGCAAGCTCGTTCCGAGATTAAGGATCTTCGACGAATGGTTGAAGAAGAAAAAAACCAAGAAAAAGAGACGCTATCAACAGAAACAGAAGGGTTAAATGTTCTGATGGAACATCAATACCCCAAGCTGCGGGTGCGCAGGACATGGGACTCTGAGAATACAACACCACTGAGCCCTATATCAGCTCACCGTTCCAGTATATCTCCACGATCAACTGAATATAGTTATGAAGAGAATGTCTTTCAGCTATCAGATTTCAGGATAGATTCTGCATCAAGTAGTCCGCAACAGCTTGCGTTTGTAACTCCTTTCCTTAAGGTTCCACTTGATGATATTCACGTGACAGATACTGTAGACCAATCTCATGTTCACAAAGAGGAAGCTATAGAAGAACCTCATGTTCAGGAGGAACGGTTTTATGAAATGGCTGAACACACTGACGGAAATTCTGAGGATAACTGCAGGGAAGTTCGGTGCATAGAAACAGAGAAGTCAGATATTAGTATAGGTCCAGTGGAAAATATGCCGGAGAGCAGTCCAGACAAATACGAGGCTGTAACTGCAGAAGAACCAGTATCTGTGACAGAGCCAAAGAACTTACAACATCCGACAGAAGAAGCAGTATGTGTGACAGAGACAAAGAACTTACAACATCCCACAGAAGCTGAAAACGAAGAAGAAGAAGAAGAAGAAAGAGTAAAGGAAGTCAGTGGTGCTTCTCCTGAACCAAAACAAGAAAGCAACCTCACAAAAAATCCTGCATTGTGTGATTTGGAATGTTCACCTGATGAGTTTGATACAAGCATGTCTAACCTGAGTAGAATCTCAACACCTCCAGCCCTTATCACTCCCTCTCCTGAAAAGCCTTTCTCTTGGATTATGGAGAGAGATTCACAACTTTTCAGAGGAATGAAGCTGACTAGAAGTCGAAGCTGCCGACCAAGCTTATTATCGAGCCCTTCCTCCTCTTGGTTAGAGAAAGATGCTGATACACCGCCAAGCTGGTACGACAAAGAATTTATCAAAACAGCTGAGAGAAACTTGACAATGTGCGACATCAAGAACCAGAGATTGTTGCAGGATGAGTTTAGTGGGAGGAGCATGCCAACAACTTGGTTCGAGAGAAGCTTGAGTGACACTCAAACGGTGGATGCTGCTTCACATGGTGTCAGCAACGAAATGTCGCCAAATGAATCACCATTTCGGCCATCTGATGCTTCTGTGTTTGAGCTCCAGACAAGTGGTAGAGCTTCAATAAGCCAGGACAGAACAGAGGAGACTGCTGCTCAAAAAGACAAGCAAATCATTCATCGATCGATGGAGGAAAGGGAACAGAAGTTCTTGGCCTCGAATTCAACGAAGAGTTTCAAGGACGCAGCAATGGACCCAATACAAGACTACTTAGACACGGCCTTGAATTGGCCAGTGGAGTTCAAGAGGCTACAGAGGGAGATAATAGAGCTATGGCACGTGTGTAAAGTCTCAATGGCACACAGAAGCTACTTCTTCCTTCTGTTCAGAGGAGATCAGAAAGATTGCTTGTACTTGGAGGTAGAGCTCCGTAGACTTAAGTACATCAGAGAATCTTTCGCCCAAAATAGCAACGATGGCAACAATATGACCCTAATCTCCTGCACGAGGGCATTGACGAGGGAGAGGTATAAGCTGAGCAAGCTGATGCAGAGGAAACTGTCGAAAGAAGAGAGAGAGAACTTGTTCTTGAGATGGGGCATTGGGCTCAACACGAATCACAGACGGGTCCAGCTGGCGCGTCGTCTCTGGTCGGATTACAAAGACATGGGTCATGTCCGAGAGAGCGCCTCCCTCGTTGGTAAGCTCAATGGGTTCGTGGACATGAAGTTGACTTCCACAGAGATGTTTGGCGTCAACTATGCTTTCAGACCACCACGTCCCAAGAAATCTAGTCTTTGGAAACGCAGCGTTTTGTCCCTCTCCTTTTTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0000278 GO:0000280 GO:0000775 GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005694 GO:0005856 GO:0005871 GO:0005875 GO:0005911 GO:0006928 GO:0006996 GO:0007010 GO:0007017 GO:0007018 GO:0007049 GO:0007051 GO:0007052 GO:0008150 GO:0009506 GO:0009987 GO:0015630 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022402 GO:0022607 GO:0030054 GO:0032991 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043515 GO:0044085 GO:0044422 GO:0044424 GO:0044427 GO:0044430 GO:0044446 GO:0044464 GO:0044877 GO:0048285 GO:0051225 GO:0055044 GO:0070925 GO:0071840 GO:0090307 GO:0098687 GO:0140014 GO:1902850 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF3490 Kinesin

Protein Analysis

1063

Amino Acids

121.42

Weight (kDa)

5.53

Isoelectric Point (pI)

58.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Microtub_bd	PF16796	19 - 157	1.1e-18	Microtubule binding
Kinesin	PF00225	24 - 342	2.5e-99	Kinesin motor domain
DUF3490	PF11995	886 - 1042	4.8e-66	Domain of unknown function (DUF3490)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003296)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G51150 AT3G51150 AT3G51150 AT3G51150 AT3G51150 AT5G66310 AT5G66310 AT5G66310
fragaria_vesca	FvH4_1g12070 FvH4_1g12070 FvH4_1g12070 FvH4_1g12070 FvH4_1g12070 FvH4_1g12070 FvH4_1g12070
malus_domestica	MD00G1176100.v1.1 MD02G1110400.v1.1 MD02G1135300.v1.1 MD15G1248100.v1.1
prunus_persica	Prupe.7G166000_v2.0.a1 Prupe.7G166000_v2.0.a1
pyrus_communis	pycom02g10640 pycom15g21790
rosa_chinensis	RchiOBHm_Chr2g0099691
rosa_laevigata	RLG00000016906
rosa_multiflora	Rmu_sc0004019.1_g000007
rosa_roxburghii	Rroxscaffold_2G00142830
rosa_rugosa	Rorug02G0082100 Rorug02G0082200 Rorug02G0082300 Rorug02G0082400
rosa_samantha	Rh2AG131700 Rh2BG135000 Rh2CG135900 Rh2DG136400
rosa_wichuraiana	Rw2G010220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	232
AasI	GACNNNNNNGTC	1 cut(s)	3135
Acc16I	TGCGCA	2 cut(s)	1015, 1364
Acc36I	ACCTGC	1 cut(s)	232
AccB1I	GGYRCC	1 cut(s)	41
AccBSI	CCGCTC	1 cut(s)	351
AccI	GTMKAC	2 cut(s)	1581, 2791
AccII	CGCG	1 cut(s)	2998
AciI	CCGC	5 cut(s)	39, 351, 1357, 1511, 2222
AclWI	GGATC	2 cut(s)	835, 1260
AcoI	YGGCCR	2 cut(s)	2432, 2648
AcsI	RAATTY	5 cut(s)	413, 686, 1672, 2243, 2578
AcuI	CTGAAG	1 cut(s)	893
AcvI	CACGTG	2 cut(s)	1567, 2698
AcyI	GRCGYC	1 cut(s)	3111
AdeI	CACNNNGTG	1 cut(s)	2393
AfaI	GTAC	7 cut(s)	235, 341, 475, 712, 2234, 2771, 2801
AfiI	CCNNNNNNNGG	8 cut(s)	121, 515, 786, 1129, 1356, 2196, 2646, 2865
AflII	CTTAAG	5 cut(s)	422, 469, 1193, 1541, 2795
AflIII	ACRYGT	1 cut(s)	2697
AhdI	GACNNNNNGTC	1 cut(s)	2151
AhlI	ACTAGT	1 cut(s)	482
AjiI	CACGTC	1 cut(s)	3137
AjnI	CCWGG	2 cut(s)	45, 2487
AjuI	GAANNNNNNNTTGG	2 cut(s)	1578, 1610
AloI	GAACNNNNNNTCC	2 cut(s)	2549, 2581
Alw21I	GWGCWC	3 cut(s)	579, 2460, 2787
Alw26I	GTCTC	9 cut(s)	549, 608, 648, 768, 1289, 1856, 2498, 2713, 3008
AlwI	GGATC	2 cut(s)	835, 1260
AlwNI	CAGNNNCTG	3 cut(s)	1577, 1805, 1892
AoxI	GGCC	4 cut(s)	2432, 2571, 2637, 2648
ApeKI	GCWGC	7 cut(s)	1011, 1354, 2160, 2381, 2510, 2603, 3165
ApoI	RAATTY	5 cut(s)	413, 686, 1672, 2243, 2578
Asp700I	GAANNNNTTC	4 cut(s)	417, 566, 2591, 2942
AspLEI	GCGC	5 cut(s)	368, 1016, 1365, 2998, 3047
AspS9I	GGNCC	4 cut(s)	32, 1736, 2611, 2986
AsuC2I	CCSGG	1 cut(s)	640
AsuHPI	GGTGA	5 cut(s)	623, 1036, 1406, 2001, 2415
AvaII	GGWCC	4 cut(s)	32, 1736, 2611, 2986
BaeGI	GKGCMC	1 cut(s)	873
BalI	TGGCCA	1 cut(s)	2650
BanI	GGYRCC	1 cut(s)	41
BanII	GRGCYC	6 cut(s)	579, 1403, 2186, 2460, 2787, 2967
BauI	CACGAG	2 cut(s)	681, 2863
BbrPI	CACGTG	2 cut(s)	1567, 2698
BbsI	GAAGAC	1 cut(s)	906
Bbv12I	GWGCWC	3 cut(s)	579, 2460, 2787
BbvI	GCAGC	7 cut(s)	998, 1341, 2147, 2368, 2497, 2615, 3177
BccI	CCATC	6 cut(s)	152, 1327, 2443, 2539, 2828, 2946
BceAI	ACGGC	1 cut(s)	2652
BciT130I	CCWGG	2 cut(s)	47, 2489
BciVI	GTATCC	1 cut(s)	198
BclI	TGATCA	1 cut(s)	19
BcnI	CCSGG	1 cut(s)	640
BcoDI	GTCTC	9 cut(s)	549, 608, 648, 768, 1289, 1856, 2498, 2713, 3008
BcuI	ACTAGT	1 cut(s)	482
BfaI	CTAG	8 cut(s)	29, 300, 483, 1125, 1214, 1226, 2148, 3150
BfmI	CTRYAG	6 cut(s)	6, 1578, 1611, 1687, 1788, 2669
BfoI	RGCGCY	1 cut(s)	3048
BfrI	CTTAAG	5 cut(s)	422, 469, 1193, 1541, 2795
BfuAI	ACCTGC	1 cut(s)	232
BfuI	GTATCC	1 cut(s)	198
BglII	AGATCT	3 cut(s)	54, 441, 463
BisI	GCNGC	8 cut(s)	40, 1012, 1355, 2161, 2382, 2511, 2604, 3166
BlpI	GCTNAGC	1 cut(s)	2894
BlsI	GCNGC	8 cut(s)	41, 1013, 1356, 2162, 2383, 2512, 2605, 3167
BmcAI	AGTACT	1 cut(s)	712
Bme1390I	CCNGG	3 cut(s)	47, 640, 2489
Bme18I	GGWCC	4 cut(s)	32, 1736, 2611, 2986
BmeRI	GACNNNNNGTC	1 cut(s)	2151
BmgBI	CACGTC	1 cut(s)	3137
BmgT120I	GGNCC	4 cut(s)	32, 1736, 2611, 2986
BmiI	GGNNCC	4 cut(s)	43, 1548, 2613, 2987
BmrFI	CCNGG	3 cut(s)	47, 640, 2489
BmrI	ACTGGG	3 cut(s)	130, 839, 876
BmsI	GCATC	7 cut(s)	15, 138, 1508, 2200, 2368, 2431, 2895
BmuI	ACTGGG	3 cut(s)	130, 839, 876
BoxI	GACNNNNGTC	1 cut(s)	3027
BpiI	GAAGAC	1 cut(s)	906
BplI	GAGNNNNNCTC	4 cut(s)	2350, 2382, 3032, 3064
BpmI	CTGGAG	2 cut(s)	2046, 2444
Bpu1102I	GCTNAGC	1 cut(s)	2894
BpuEI	CTTGAG	3 cut(s)	1199, 2377, 2968
BpuMI	CCSGG	1 cut(s)	640
Bsa29I	ATCGAT	2 cut(s)	2539, 2543
BsaAI	YACGTR	2 cut(s)	1567, 2698
BsaBI	GATNNNNATC	1 cut(s)	18
BsaHI	GRCGYC	1 cut(s)	3111
BsaI	GGTCTC	1 cut(s)	648
BsaJI	CCNNGG	2 cut(s)	45, 369
BsaXI	ACNNNNNCTCC	4 cut(s)	1625, 1655, 2065, 2095
Bsc4I	CCNNNNNNNGG	8 cut(s)	121, 515, 786, 1129, 1356, 2196, 2646, 2865
Bse118I	RCCGGY	1 cut(s)	779
Bse1I	ACTGG	9 cut(s)	125, 563, 834, 871, 1107, 1422, 1739, 1799, 2651
Bse3DI	GCAATG	1 cut(s)	2613
Bse8I	GATNNNNATC	1 cut(s)	18
BseBI	CCWGG	2 cut(s)	47, 2489
BseCI	ATCGAT	2 cut(s)	2539, 2543
BseDI	CCNNGG	2 cut(s)	45, 369
BseGI	GGATG	4 cut(s)	1831, 1879, 2314, 2383
BseJI	GATNNNNATC	1 cut(s)	18
BseLI	CCNNNNNNNGG	8 cut(s)	121, 515, 786, 1129, 1356, 2196, 2646, 2865
BseMI	GCAATG	1 cut(s)	2613
BseMII	CTCAG	9 cut(s)	216, 501, 505, 1371, 1388, 1668, 2033, 2250, 2885
BseNI	ACTGG	9 cut(s)	125, 563, 834, 871, 1107, 1422, 1739, 1799, 2651
BseRI	GAGGAG	4 cut(s)	2182, 2338, 2516, 2763
BseSI	GKGCMC	1 cut(s)	873
BseXI	GCAGC	7 cut(s)	998, 1341, 2147, 2368, 2497, 2615, 3177
BsgI	GTGCAG	1 cut(s)	2845
Bsh1236I	CGCG	1 cut(s)	2998
Bsh1285I	CGRYCG	1 cut(s)	2543
BshFI	GGCC	4 cut(s)	2434, 2573, 2639, 2650
BshNI	GGYRCC	1 cut(s)	41
BshVI	ATCGAT	2 cut(s)	2539, 2543
BsiEI	CGRYCG	1 cut(s)	2543
BsiHKAI	GWGCWC	3 cut(s)	579, 2460, 2787
BsiSI	CCGG	3 cut(s)	640, 780, 1754
BslFI	GGGAC	5 cut(s)	98, 532, 1388, 3123, 3160
BslI	CCNNNNNNNGG	8 cut(s)	121, 515, 786, 1129, 1356, 2196, 2646, 2865
BsmAI	GTCTC	9 cut(s)	549, 608, 648, 768, 1289, 1856, 2498, 2713, 3008
BsmBI	CGTCTC	3 cut(s)	768, 1289, 3008
BsmFI	GGGAC	5 cut(s)	98, 532, 1388, 3123, 3160
BsnI	GGCC	4 cut(s)	2434, 2573, 2639, 2650
Bso31I	GGTCTC	1 cut(s)	648
Bsp1286I	GDGCHC	7 cut(s)	579, 873, 1403, 2186, 2460, 2787, 2967
Bsp1407I	TGTACA	1 cut(s)	233
Bsp1720I	GCTNAGC	1 cut(s)	2894
BspACI	CCGC	5 cut(s)	39, 351, 1357, 1511, 2222
BspANI	GGCC	4 cut(s)	2434, 2573, 2639, 2650
BspCNI	CTCAG	9 cut(s)	217, 502, 504, 1372, 1389, 1669, 2034, 2251, 2886
BspDI	ATCGAT	2 cut(s)	2539, 2543
BspFNI	CGCG	1 cut(s)	2998
BspLI	GGNNCC	4 cut(s)	43, 1548, 2613, 2987
BspMAI	CTGCAG	2 cut(s)	1691, 1792
BspMI	ACCTGC	1 cut(s)	232
BspPI	GGATC	2 cut(s)	835, 1260
BspT107I	GGYRCC	1 cut(s)	41
BspTI	CTTAAG	5 cut(s)	422, 469, 1193, 1541, 2795
BspTNI	GGTCTC	1 cut(s)	648
BsrBI	CCGCTC	1 cut(s)	351
BsrDI	GCAATG	1 cut(s)	2613
BsrFI	RCCGGY	1 cut(s)	779
BsrGI	TGTACA	1 cut(s)	233
BsrI	ACTGG	9 cut(s)	125, 563, 834, 871, 1107, 1422, 1739, 1799, 2651
BssAI	RCCGGY	1 cut(s)	779
BssECI	CCNNGG	2 cut(s)	45, 369
BssNI	GRCGYC	1 cut(s)	3111
BssSI	CACGAG	2 cut(s)	681, 2863
BssT1I	CCWWGG	1 cut(s)	369
Bst2BI	CACGAG	2 cut(s)	681, 2863
Bst2UI	CCWGG	2 cut(s)	47, 2489
Bst6I	CTCTTC	3 cut(s)	1452, 2582, 2922
BstACI	GRCGYC	1 cut(s)	3111
BstAFI	CTTAAG	5 cut(s)	422, 469, 1193, 1541, 2795
BstAPI	GCANNNNNTGC	1 cut(s)	938
BstAUI	TGTACA	1 cut(s)	233
BstBAI	YACGTR	2 cut(s)	1567, 2698
BstC8I	GCNNGC	7 cut(s)	781, 1009, 1233, 1521, 2330, 2900, 2994
BstENI	CCTNNNNNAGG	2 cut(s)	513, 2863
BstF5I	GGATG	4 cut(s)	1831, 1879, 2314, 2383
BstFNI	CGCG	1 cut(s)	2998
BstH2I	RGCGCY	1 cut(s)	3048
BstHHI	GCGC	5 cut(s)	368, 1016, 1365, 2998, 3047
BstMAI	GTCTC	9 cut(s)	549, 608, 648, 768, 1289, 1856, 2498, 2713, 3008
BstMCI	CGRYCG	1 cut(s)	2543
BstMWI	GCNNNNNNNGC	7 cut(s)	938, 1013, 1360, 1787, 2169, 2835, 2899
BstNI	CCWGG	2 cut(s)	47, 2489
BstNSI	RCATGY	2 cut(s)	2035, 2332
BstPAI	GACNNNNGTC	1 cut(s)	3027
BstSCI	CCNGG	3 cut(s)	45, 638, 2487
BstSFI	CTRYAG	6 cut(s)	6, 1578, 1611, 1687, 1788, 2669
BstSLI	GKGCMC	1 cut(s)	873
BstUI	CGCG	1 cut(s)	2998
BstV1I	GCAGC	7 cut(s)	998, 1341, 2147, 2368, 2497, 2615, 3177
BstV2I	GAAGAC	1 cut(s)	906
BstX2I	RGATCY	4 cut(s)	54, 441, 463, 1252
BstXI	CCANNNNNNTGG	2 cut(s)	2340, 2468
BstYI	RGATCY	4 cut(s)	54, 441, 463, 1252
Bsu15I	ATCGAT	2 cut(s)	2539, 2543
BsuI	GTATCC	1 cut(s)	198
BsuRI	GGCC	4 cut(s)	2434, 2573, 2639, 2650
BsuTUI	ATCGAT	2 cut(s)	2539, 2543
BtrI	CACGTC	1 cut(s)	3137
BtsCI	GGATG	4 cut(s)	1831, 1879, 2314, 2383
BtsI	GCAGTG	1 cut(s)	334
BveI	ACCTGC	1 cut(s)	232
Cac8I	GCNNGC	7 cut(s)	781, 1009, 1233, 1521, 2330, 2900, 2994
CaiI	CAGNNNCTG	3 cut(s)	1577, 1805, 1892
CfoI	GCGC	5 cut(s)	368, 1016, 1365, 2998, 3047
Cfr10I	RCCGGY	1 cut(s)	779
Cfr13I	GGNCC	4 cut(s)	32, 1736, 2611, 2986
ClaI	ATCGAT	2 cut(s)	2539, 2543
CseI	GACGC	5 cut(s)	750, 1306, 2609, 2987, 3100
Csp6I	GTAC	7 cut(s)	234, 340, 474, 711, 2233, 2770, 2800
CviQI	GTAC	7 cut(s)	234, 340, 474, 711, 2233, 2770, 2800
DraI	TTTAAA	1 cut(s)	84
DraIII	CACNNNGTG	1 cut(s)	2393
DrdI	GACNNNNNNGTC	1 cut(s)	3135
DriI	GACNNNNNGTC	1 cut(s)	2151
DseDI	GACNNNNNNGTC	1 cut(s)	3135
EaeI	YGGCCR	2 cut(s)	2432, 2648
Eam1104I	CTCTTC	3 cut(s)	1452, 2582, 2922
Eam1105I	GACNNNNNGTC	1 cut(s)	2151
EarI	CTCTTC	3 cut(s)	1452, 2582, 2922
Ecl136II	GAGCTC	3 cut(s)	577, 2458, 2785
Eco130I	CCWWGG	1 cut(s)	369
Eco24I	GRGCYC	6 cut(s)	579, 1403, 2186, 2460, 2787, 2967
Eco31I	GGTCTC	1 cut(s)	648
Eco47I	GGWCC	4 cut(s)	32, 1736, 2611, 2986
Eco53kI	GAGCTC	3 cut(s)	577, 2458, 2785
Eco57I	CTGAAG	1 cut(s)	893
Eco72I	CACGTG	2 cut(s)	1567, 2698
EcoICRI	GAGCTC	3 cut(s)	577, 2458, 2785
EcoNI	CCTNNNNNAGG	2 cut(s)	513, 2863
EcoRI	GAATTC	2 cut(s)	413, 2578
EcoRII	CCWGG	2 cut(s)	45, 2487
EcoT14I	CCWWGG	1 cut(s)	369
EcoT38I	GRGCYC	6 cut(s)	579, 1403, 2186, 2460, 2787, 2967
ErhI	CCWWGG	1 cut(s)	369
Esp3I	CGTCTC	3 cut(s)	768, 1289, 3008
FaqI	GGGAC	5 cut(s)	98, 532, 1388, 3123, 3160
FauI	CCCGC	1 cut(s)	1350
FauNDI	CATATG	1 cut(s)	313
FbaI	TGATCA	1 cut(s)	19
FblI	GTMKAC	2 cut(s)	1581, 2791
Fnu4HI	GCNGC	8 cut(s)	40, 1012, 1355, 2161, 2382, 2511, 2604, 3166
FokI	GGATG	4 cut(s)	1818, 1866, 2321, 2390
FriOI	GRGCYC	6 cut(s)	579, 1403, 2186, 2460, 2787, 2967
Fsp4HI	GCNGC	8 cut(s)	40, 1012, 1355, 2161, 2382, 2511, 2604, 3166
FspBI	CTAG	8 cut(s)	29, 300, 483, 1125, 1214, 1226, 2148, 3150
FspI	TGCGCA	2 cut(s)	1015, 1364
GlaI	GCGC	5 cut(s)	367, 1015, 1364, 2997, 3046
GluI	GCNGC	8 cut(s)	40, 1012, 1355, 2161, 2382, 2511, 2604, 3166
GsuI	CTGGAG	2 cut(s)	2046, 2444
HaeII	RGCGCY	1 cut(s)	3048
HaeIII	GGCC	4 cut(s)	2434, 2573, 2639, 2650
HapII	CCGG	3 cut(s)	640, 780, 1754
HgaI	GACGC	5 cut(s)	750, 1306, 2609, 2987, 3100
HhaI	GCGC	5 cut(s)	368, 1016, 1365, 2998, 3047
Hin1I	GRCGYC	1 cut(s)	3111
Hin6I	GCGC	5 cut(s)	366, 1014, 1363, 2996, 3045
HinP1I	GCGC	5 cut(s)	366, 1014, 1363, 2996, 3045
HincII	GTYRAC	3 cut(s)	825, 3090, 3115
HindII	GTYRAC	3 cut(s)	825, 3090, 3115
HindIII	AAGCTT	4 cut(s)	1065, 1190, 2170, 2353
HpaI	GTTAAC	1 cut(s)	825
HpaII	CCGG	3 cut(s)	640, 780, 1754
HphI	GGTGA	5 cut(s)	623, 1036, 1406, 2001, 2415
Hpy99I	CGWCG	2 cut(s)	1263, 3003
HpyAV	CCTTC	5 cut(s)	201, 791, 1310, 2196, 2747
HpyCH4IV	ACGT	5 cut(s)	338, 757, 1566, 2697, 3136
HpyF10VI	GCNNNNNNNGC	7 cut(s)	938, 1013, 1360, 1787, 2169, 2835, 2899
HpySE526I	ACGT	5 cut(s)	338, 757, 1566, 2697, 3136
Hsp92I	GRCGYC	1 cut(s)	3111
HspAI	GCGC	5 cut(s)	366, 1014, 1363, 2996, 3045
KroI	GCCGGC	1 cut(s)	779
KroNI	GCCGGC	1 cut(s)	781
Ksp22I	TGATCA	1 cut(s)	19
KspAI	GTTAAC	1 cut(s)	825
LmnI	GCTCC	6 cut(s)	574, 582, 641, 2325, 2463, 2790
Lsp1109I	GCAGC	7 cut(s)	998, 1341, 2147, 2368, 2497, 2615, 3177
LweI	GCATC	7 cut(s)	15, 138, 1508, 2200, 2368, 2431, 2895
MaeI	CTAG	8 cut(s)	29, 300, 483, 1125, 1214, 1226, 2148, 3150
MaeII	ACGT	5 cut(s)	338, 757, 1566, 2697, 3136
MaeIII	GTNAC	7 cut(s)	1024, 1528, 1567, 1783, 1807, 1855, 2360
MbiI	CCGCTC	1 cut(s)	351
MfeI	CAATTG	1 cut(s)	1137
MflI	RGATCY	4 cut(s)	54, 441, 463, 1252
MhlI	GDGCHC	7 cut(s)	579, 873, 1403, 2186, 2460, 2787, 2967
MlsI	TGGCCA	1 cut(s)	2650
MluNI	TGGCCA	1 cut(s)	2650
MlyI	GAGTC	4 cut(s)	166, 461, 967, 1370
MmeI	TCCRAC	3 cut(s)	1234, 1859, 2991
Mox20I	TGGCCA	1 cut(s)	2650
MroNI	GCCGGC	1 cut(s)	779
MroXI	GAANNNNTTC	4 cut(s)	417, 566, 2591, 2942
MscI	TGGCCA	1 cut(s)	2650
MslI	CAYNNNNRTG	1 cut(s)	3101
Msp20I	TGGCCA	1 cut(s)	2650
MspA1I	CMGCKG	2 cut(s)	2258, 2992
MspCI	CTTAAG	5 cut(s)	422, 469, 1193, 1541, 2795
MspI	CCGG	3 cut(s)	640, 780, 1754
MspR9I	CCNGG	3 cut(s)	47, 640, 2489
MunI	CAATTG	1 cut(s)	1137
MvaI	CCWGG	2 cut(s)	47, 2489
MvnI	CGCG	1 cut(s)	2998
MwoI	GCNNNNNNNGC	7 cut(s)	938, 1013, 1360, 1787, 2169, 2835, 2899
NaeI	GCCGGC	1 cut(s)	781
NciI	CCSGG	1 cut(s)	640
NdeI	CATATG	1 cut(s)	313
NgoMIV	GCCGGC	1 cut(s)	779
NlaIV	GGNNCC	4 cut(s)	43, 1548, 2613, 2987
NmuCI	GTSAC	5 cut(s)	1024, 1567, 1807, 1855, 2360
NsbI	TGCGCA	2 cut(s)	1015, 1364
NspI	RCATGY	2 cut(s)	2035, 2332
PaeI	GCATGC	1 cut(s)	2332
PaqCI	CACCTGC	1 cut(s)	232
PcsI	WCGNNNNNNNCGW	2 cut(s)	2161, 2582
PdiI	GCCGGC	1 cut(s)	781
PdmI	GAANNNNTTC	4 cut(s)	417, 566, 2591, 2942
PfeI	GAWTC	9 cut(s)	562, 887, 969, 1493, 2049, 2117, 2420, 2810, 2974
PkrI	GCNGC	8 cut(s)	41, 1013, 1356, 2162, 2383, 2512, 2605, 3167
Ple19I	CGATCG	1 cut(s)	2543
PleI	GAGTC	4 cut(s)	165, 460, 966, 1370
PmaCI	CACGTG	2 cut(s)	1567, 2698
PmlI	CACGTG	2 cut(s)	1567, 2698
PpsI	GAGTC	4 cut(s)	165, 460, 966, 1370
Ppu21I	YACGTR	2 cut(s)	1567, 2698
PshAI	GACNNNNGTC	1 cut(s)	3027
Psp124BI	GAGCTC	3 cut(s)	579, 2460, 2787
Psp6I	CCWGG	2 cut(s)	45, 2487
PspCI	CACGTG	2 cut(s)	1567, 2698
PspGI	CCWGG	2 cut(s)	45, 2487
PspN4I	GGNNCC	4 cut(s)	43, 1548, 2613, 2987
PspPI	GGNCC	4 cut(s)	32, 1736, 2611, 2986
PstI	CTGCAG	2 cut(s)	1691, 1792
PstNI	CAGNNNCTG	3 cut(s)	1577, 1805, 1892
PsuI	RGATCY	4 cut(s)	54, 441, 463, 1252
PvuI	CGATCG	1 cut(s)	2543
PvuII	CAGCTG	2 cut(s)	2258, 2992
RsaI	GTAC	7 cut(s)	235, 341, 475, 712, 2234, 2771, 2801
RsaNI	GTAC	7 cut(s)	234, 340, 474, 711, 2233, 2770, 2800
RseI	CAYNNNNRTG	1 cut(s)	3101
SacI	GAGCTC	3 cut(s)	579, 2460, 2787
SatI	GCNGC	8 cut(s)	40, 1012, 1355, 2161, 2382, 2511, 2604, 3166
Sau96I	GGNCC	4 cut(s)	32, 1736, 2611, 2986
ScaI	AGTACT	1 cut(s)	712
SchI	GAGTC	4 cut(s)	166, 461, 967, 1370
ScrFI	CCNGG	3 cut(s)	47, 640, 2489
SduI	GDGCHC	7 cut(s)	579, 873, 1403, 2186, 2460, 2787, 2967
SfaNI	GCATC	7 cut(s)	15, 138, 1508, 2200, 2368, 2431, 2895
SfcI	CTRYAG	6 cut(s)	6, 1578, 1611, 1687, 1788, 2669
SinI	GGWCC	4 cut(s)	32, 1736, 2611, 2986
SmiMI	CAYNNNNRTG	1 cut(s)	3101
SmlI	CTYRAG	8 cut(s)	422, 469, 1178, 1193, 1541, 2356, 2795, 2947
SmoI	CTYRAG	8 cut(s)	422, 469, 1178, 1193, 1541, 2356, 2795, 2947
SpeI	ACTAGT	1 cut(s)	482
SphI	GCATGC	1 cut(s)	2332
SsiI	CCGC	5 cut(s)	39, 351, 1357, 1511, 2222
SspMI	CTAG	8 cut(s)	29, 300, 483, 1125, 1214, 1226, 2148, 3150
SstI	GAGCTC	3 cut(s)	579, 2460, 2787
StyD4I	CCNGG	3 cut(s)	45, 638, 2487
StyI	CCWWGG	1 cut(s)	369
TaiI	ACGT	5 cut(s)	341, 760, 1569, 2700, 3139
TaqI	TCGA	8 cut(s)	1258, 2155, 2180, 2346, 2539, 2543, 2576, 2921
TatI	WGTACW	5 cut(s)	233, 473, 710, 2769, 2799
TauI	GCSGC	1 cut(s)	42
TfiI	GAWTC	9 cut(s)	562, 887, 969, 1493, 2049, 2117, 2420, 2810, 2974
TseFI	GTSAC	5 cut(s)	1024, 1567, 1807, 1855, 2360
TseI	GCWGC	7 cut(s)	1011, 1354, 2160, 2381, 2510, 2603, 3165
Tsp45I	GTSAC	5 cut(s)	1024, 1567, 1807, 1855, 2360
TspGWI	ACGGA	4 cut(s)	833, 1181, 1683, 2777
Vha464I	CTTAAG	5 cut(s)	422, 469, 1193, 1541, 2795
VpaK11BI	GGWCC	4 cut(s)	32, 1736, 2611, 2986
XagI	CCTNNNNNAGG	2 cut(s)	513, 2863
XapI	RAATTY	5 cut(s)	413, 686, 1672, 2243, 2578
XceI	RCATGY	2 cut(s)	2035, 2332
XmiI	GTMKAC	2 cut(s)	1581, 2791
XmnI	GAANNNNTTC	4 cut(s)	417, 566, 2591, 2942
XspI	CTAG	8 cut(s)	29, 300, 483, 1125, 1214, 1226, 2148, 3150
ZrmI	AGTACT	1 cut(s)	712

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.