Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0003296

Physical Location & Seq

Genomic Coordinates Reverse (-)

20653359 .. 20659833

Gene Structure 6475 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1248100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2892 bp

ATGGTGGCCATTGGTGGCGAGGAACCAATGCAAGAGCCAGCTGGCCATGGGGAGAGAATACTTGTTTCAGTCCGGCTGCGGCCTCTGGTTGAGAAGGAGACTGCTAGAAATGATGTGTCGGATTGGGAATGCATTAATGACGCCACTATCATATACAGGAACAATCTTTCAGTTTCTGAACGCTCCATGTACCCAACTGCCTATTCATTTGACAGAGTATTTGCGACTGACTGCTCAACAAGGCGGGTGTACGAAGAAGGAGCCAAGAGAGTTGCTATTTCAGTTGTCAGCGGTATAAACTCAAGTATCTTTGCATATGGACAAACAAGCAGTGGAAAGACGTATACCATGAGTGGCATTACTGATTACGCTGTTGCCGACATATATGACTACATAGAGAAGCACACGGAGAGAGAATTCAATTTGAAGTTCTCTGCCATGGAGATCTACAACGAATCTGTTAGGGACCTCCTTAGTGCAGATACCACTCCGCTGAGACTTCTAGATGATCCAGAGAGAGGTACAATTGTTGAGAAACTCACCGAGGAAACTCTAACGGACTGGAACCATTTCAAAGAACTTCTATCTATCTGCGAAGCTCAAAGACAGATAGGGGAAACAGCCCTAAATGAAGCAAGCTCCAGATCTCATCAGATTCTCAGATTGGTAATTGAAAGTTCAGCACGTGAGTTTCTTGGCTACGACAAATCAAGCTCTCTTACAGCTGTTGTGAACTTTGTCGATCTTGCTGGAAGTGAACGTGCATCCCAGACGTTATCAGCTGGAACAAGGTTGAAAGAGGGTTGCCACATAAATCGCAGTTTACTAACTCTGGGCACTGTTATCCGTAAGCTCAGCAAGGGAAGAAGTGGGCATGTTCCTTACAGAGATTCAAAGCTAACCCGCATACTGCAGTCCTCGTTGGGAGGAAATGCTAGAACTGCCATTATCTGTACCATGAGTCCTGCACATAGCCATGTTGAGCAATCAAGAAATACCCTCTTATTTGCGAGCTGTGCTAAAGAAGTGACAACAAGCGCACAGGTTAACGTGGTCATGTCGGATAAAGCATTGGTGAAGCATTTGCAGAAAGAATTGACTAGACTAGAGAATGAGTTGAGAGGTTCAGGACACAGAATTGTTTCAGCTGATTCTTCAACATTACTGAGAGAAAAGGACCTTCAGATTGAAAAGCTCAAGAAAGACGTATCGGAGCTAACTCAGCAACGAGACCTTGCTCAATCTCAGGTTAAGGATCTGGTGCGAGTGCTTGGAGATGATAAACCATCAGAAGAGGATCTGGAACGTTATTACCCCAAACTACGTGTGCGAAATTCATGGGACTTTGAAATGCAAAAGGCAGGGGCGCCTGTTCTGGCCGTCTCTCGCAGACAAGCCAACAGTGTGAGATCTTTTGGCACCTCTCAGTATTCTGATGGAGACAGTAGGACTAGTTCAGAGGAGACAATGTTCCAACTTCGCGACATGGAAGAAAATTTCATGCACACTGATTCCTCCCGGCAGTTATCAGATGGCATTCCTAATTTTTCTTTCGGTATTCCGCATCAAGAGGAGAGCAAGGAACAGTCTGATGGAAATTCGGAGGATCTCTGCAAGGAAGTTCGTTGTATTGAAATGGAAGAGTCTGGCACCAACAGATATACAGCGTCAAATATATCAGATTCAAGTGCTAGCAGATACCAAAACTCAAATATGTCGTCTCCTGTAGCAAACACAGCTACCTCAGGACTGACAACGGTCGGAAATGGAGATGGCACAAATCAGGAATTGGAGTCACCCCTACCTAAACAAAAAGGTTTTATAGTTCCATCTTCTGAAAGGACACCTCAATGGCTTCCTGAGAAAGAAATGTTTATCCCCTCGGTTTTGATGTTAAGGAGGAGTCGAAGTTGCAAAGCAAGGCTTATGACTAATTCATCGCCATGGTGGTTTGAGTTGGTGGAGAAGAATGAGAGCACACCCGCTACTGAGTTTGAGAAAAGTTTTATAGGAAGGCCCGAGGGTTTCCAAAAGAAACTTCCTTCACTGAACTATGGTGCTGAGACTGAAAGTCTATCACGAAATGCTTCTCAGGCTTCTGCAAGAAGTGCTGCTATTCGTGACAGTAAAGCACAGGATACAAAATCCACAGCTGATGACAAGATAACTGAAAGCACTACTTTAGTAGAAGGAACAGAGGAAACGACCGATACCCAATGCAATACTCAATTTTCTGATTCTATGGTACCGGAGACAGACTCAAAGCCCATACCATCTTTAAGGGATGTAAAAGATGTTGGCTTGGACCCAATGCCTATTGATGACGAAAACCCTTCAAGGTGGCCTTCAGAATTCAACAAGCTGCAAAGAGAGATCATTGAACTTTGGGATGCCTGCAACGTGTCATTGGTTCACAGGACTTACTTCTTCCTCCTATTTAAAGGTGACCCAAGTGATTCTATTTACATGGAGGTTGAGCTCAGGAGATTGTCCTTCCTCAAAGACACATTTTTGCGTGGAAATCAGACTTTGGAAGATGGCCAAACTCTAACACCCGCTTCAAGCATGAAGGCTTTGACGAGTGAGAGAGACTTGCTGAGCAAGCAAATGCGAAGGCGACTCTCCGAGGATGAAAGAAATAATCTCTACCTAAAGTGGGGTATCAGTCTACATTCGAAGCATAGGCGGTTGCAATTGGCAAACCTCCTGTGGAATAACACACAAAACCTGGACCACATCATGGATAGTGCCACAATTGTTGCAAAGTTGGTAGGTTCAGTAGAGCCCGAGCAAGCATTCAAGGAGATGTTTGGACTCAGATTTGCACCGCGAGACACTGTCGCCAAGCGGAAATCTCACTTTTGGTCAGAAAGTTTCAAGTCTCTTGTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0000278 GO:0000280 GO:0000775 GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005694 GO:0005856 GO:0005871 GO:0005875 GO:0005911 GO:0006928 GO:0006996 GO:0007010 GO:0007017 GO:0007018 GO:0007049 GO:0007051 GO:0007052 GO:0008150 GO:0009506 GO:0009987 GO:0015630 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022402 GO:0022607 GO:0030054 GO:0032991 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043515 GO:0044085 GO:0044422 GO:0044424 GO:0044427 GO:0044430 GO:0044446 GO:0044464 GO:0044877 GO:0048285 GO:0051225 GO:0055044 GO:0070925 GO:0071840 GO:0090307 GO:0098687 GO:0140014 GO:1902850 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF3490 Kinesin

Protein Analysis

964

Amino Acids

108.42

Weight (kDa)

5.64

Isoelectric Point (pI)

54.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Kinesin	PF00225	25 - 343	6e-99	Kinesin motor domain
Microtub_bd	PF16796	47 - 158	1.7e-20	Microtubule binding
DUF3490	PF11995	784 - 943	2.3e-75	Domain of unknown function (DUF3490)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003296)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G51150 AT3G51150 AT3G51150 AT3G51150 AT3G51150 AT5G66310 AT5G66310 AT5G66310
fragaria_vesca	FvH4_1g12070 FvH4_1g12070 FvH4_1g12070 FvH4_1g12070 FvH4_1g12070 FvH4_1g12070 FvH4_1g12070
malus_domestica	MD00G1176100.v1.1 MD02G1110400.v1.1 MD02G1135300.v1.1 MD15G1248100.v1.1
prunus_persica	Prupe.7G166000_v2.0.a1 Prupe.7G166000_v2.0.a1
pyrus_communis	pycom02g10640 pycom15g21790
rosa_chinensis	RchiOBHm_Chr2g0099691
rosa_laevigata	RLG00000016906
rosa_multiflora	Rmu_sc0004019.1_g000007
rosa_roxburghii	Rroxscaffold_2G00142830
rosa_rugosa	Rorug02G0082100 Rorug02G0082200 Rorug02G0082300 Rorug02G0082400
rosa_samantha	Rh2AG131700 Rh2BG135000 Rh2CG135900 Rh2DG136400
rosa_wichuraiana	Rw2G010220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	2245
AccB1I	GGYRCC	4 cut(s)	1366, 1418, 1649, 2245
AccB7I	CCANNNNNTGG	1 cut(s)	2740
AccI	GTMKAC	2 cut(s)	344, 2668
AccII	CGCG	2 cut(s)	1482, 2830
AclI	AACGTT	1 cut(s)	1306
AclWI	GGATC	4 cut(s)	503, 1263, 1305, 1614
AcoI	YGGCCR	4 cut(s)	6, 43, 1377, 2539
AcsI	RAATTY	5 cut(s)	416, 1333, 1495, 1597, 2351
AcuI	CTGAAG	2 cut(s)	1166, 2331
AcvI	CACGTG	1 cut(s)	686
AcyI	GRCGYC	2 cut(s)	141, 1367
AfaI	GTAC	5 cut(s)	191, 251, 523, 955, 2247
AfiI	CCNNNNNNNGG	3 cut(s)	518, 2656, 2740
AflIII	ACRYGT	2 cut(s)	1324, 2400
AhdI	GACNNNNNGTC	1 cut(s)	2070
AhlI	ACTAGT	1 cut(s)	1451
AjnI	CCWGG	1 cut(s)	2727
AjuI	GAANNNNNNNTTGG	4 cut(s)	187, 219, 2022, 2054
AloI	GAACNNNNNNTCC	2 cut(s)	1454, 1486
Alw21I	GWGCWC	2 cut(s)	1979, 2481
AlwI	GGATC	4 cut(s)	503, 1263, 1305, 1614
AlwNI	CAGNNNCTG	1 cut(s)	176
Ama87I	CYCGRG	2 cut(s)	2018, 2786
AoxI	GGCC	7 cut(s)	6, 43, 80, 1377, 2015, 2342, 2539
ApeKI	GCWGC	3 cut(s)	76, 2111, 2362
ApoI	RAATTY	5 cut(s)	416, 1333, 1495, 1597, 2351
ArsI	GACNNNNNNTTYG	4 cut(s)	203, 235, 1703, 1735
AseI	ATTAAT	1 cut(s)	135
Asp700I	GAANNNNTTC	3 cut(s)	569, 1141, 2086
Asp718I	GGTACC	1 cut(s)	2245
AspLEI	GCGC	2 cut(s)	1040, 1369
AspS9I	GGNCC	5 cut(s)	466, 1177, 2016, 2305, 2731
AsuC2I	CCSGG	1 cut(s)	1519
AsuHPI	GGTGA	4 cut(s)	532, 1087, 1788, 2456
AsuII	TTCGAA	1 cut(s)	2675
AsuNHI	GCTAGC	1 cut(s)	1691
AvaI	CYCGRG	2 cut(s)	2018, 2786
AvaII	GGWCC	4 cut(s)	466, 1177, 2305, 2731
AxyI	CCTNAGG	1 cut(s)	1744
BaeGI	GKGCMC	1 cut(s)	839
BaeI	ACNNNNGTAYC	4 cut(s)	289, 322, 513, 546
BalI	TGGCCA	3 cut(s)	8, 45, 2541
BanI	GGYRCC	4 cut(s)	1366, 1418, 1649, 2245
BanII	GRGCYC	2 cut(s)	2481, 2787
BbrPI	CACGTG	1 cut(s)	686
Bbv12I	GWGCWC	2 cut(s)	1979, 2481
BbvI	GCAGC	3 cut(s)	63, 2098, 2349
BccI	CCATC	8 cut(s)	1294, 1430, 1526, 1586, 1766, 1837, 2281, 2531
BceAI	ACGGC	1 cut(s)	1364
BciT130I	CCWGG	1 cut(s)	2729
BciVI	GTATCC	1 cut(s)	2131
BcnI	CCSGG	1 cut(s)	1519
BcuI	ACTAGT	1 cut(s)	1451
BfaI	CTAG	7 cut(s)	105, 503, 936, 1101, 1106, 1452, 1692
BfmI	CTRYAG	2 cut(s)	911, 1725
BfoI	RGCGCY	1 cut(s)	1370
BfuI	GTATCC	1 cut(s)	2131
BglII	AGATCT	3 cut(s)	444, 644, 1409
BisI	GCNGC	4 cut(s)	77, 80, 2112, 2363
BlpI	GCTNAGC	2 cut(s)	854, 2597
BlsI	GCNGC	4 cut(s)	78, 81, 2113, 2364
Bme1390I	CCNGG	2 cut(s)	1519, 2729
Bme18I	GGWCC	4 cut(s)	466, 1177, 2305, 2731
BmeRI	GACNNNNNGTC	1 cut(s)	2070
BmeT110I	CYCGRG	2 cut(s)	2018, 2786
BmgT120I	GGNCC	5 cut(s)	466, 1177, 2016, 2305, 2731
BmiI	GGNNCC	9 cut(s)	24, 262, 467, 566, 1368, 1420, 1651, 2247, 2307
BmrFI	CCNGG	2 cut(s)	1519, 2729
BmsI	GCATC	3 cut(s)	773, 1573, 2380
BmtI	GCTAGC	1 cut(s)	1695
BoxI	GACNNNNGTC	1 cut(s)	1757
BplI	GAGNNNNNCTC	2 cut(s)	2243, 2275
BpmI	CTGGAG	1 cut(s)	625
Bpu10I	CCTNAGC	1 cut(s)	2480
Bpu1102I	GCTNAGC	2 cut(s)	854, 2597
Bpu14I	TTCGAA	1 cut(s)	2675
BpuEI	CTTGAG	2 cut(s)	286, 1181
BpuMI	CCSGG	1 cut(s)	1519
BsaAI	YACGTR	2 cut(s)	686, 1325
BsaHI	GRCGYC	2 cut(s)	141, 1367
BsaI	GGTCTC	1 cut(s)	1224
BsaJI	CCNNGG	7 cut(s)	46, 438, 543, 1881, 1943, 2019, 2625
BsaWI	WCCGGW	1 cut(s)	2248
BsaXI	ACNNNNNCTCC	4 cut(s)	1454, 1484, 1894, 1924
Bsc4I	CCNNNNNNNGG	3 cut(s)	518, 2656, 2740
Bse1I	ACTGG	1 cut(s)	566
Bse21I	CCTNAGG	1 cut(s)	1744
BseBI	CCWGG	1 cut(s)	2729
BseDI	CCNNGG	7 cut(s)	46, 438, 543, 1881, 1943, 2019, 2625
BseGI	GGATG	4 cut(s)	764, 2290, 2395, 2635
BseLI	CCNNNNNNNGG	3 cut(s)	518, 2656, 2740
BseNI	ACTGG	1 cut(s)	566
BseRI	GAGGAG	3 cut(s)	1475, 1586, 1915
BseSI	GKGCMC	1 cut(s)	839
BseXI	GCAGC	3 cut(s)	63, 2098, 2349
BsgI	GTGCAG	2 cut(s)	498, 951
Bsh1236I	CGCG	2 cut(s)	1482, 2830
Bsh1285I	CGRYCG	2 cut(s)	1761, 2208
BshFI	GGCC	7 cut(s)	8, 45, 82, 1379, 2017, 2344, 2541
BshNI	GGYRCC	4 cut(s)	1366, 1418, 1649, 2245
BsiEI	CGRYCG	2 cut(s)	1761, 2208
BsiHKAI	GWGCWC	2 cut(s)	1979, 2481
BsiHKCI	CYCGRG	2 cut(s)	2018, 2786
BsiSI	CCGG	3 cut(s)	73, 1519, 2249
BslFI	GGGAC	2 cut(s)	479, 1355
BslI	CCNNNNNNNGG	3 cut(s)	518, 2656, 2740
BsmBI	CGTCTC	2 cut(s)	1387, 1725
BsmFI	GGGAC	2 cut(s)	479, 1355
BsmI	GAATGC	3 cut(s)	134, 1536, 2795
BsnI	GGCC	7 cut(s)	8, 45, 82, 1379, 2017, 2344, 2541
Bso31I	GGTCTC	1 cut(s)	1224
BsoBI	CYCGRG	2 cut(s)	2018, 2786
Bsp119I	TTCGAA	1 cut(s)	2675
Bsp1286I	GDGCHC	4 cut(s)	839, 1979, 2481, 2787
Bsp143I	GATC	9 cut(s)	444, 508, 644, 742, 1255, 1297, 1409, 1606, 2373
Bsp1720I	GCTNAGC	2 cut(s)	854, 2597
Bsp19I	CCATGG	3 cut(s)	46, 438, 1943
Bsp68I	TCGCGA	1 cut(s)	1482
BspANI	GGCC	7 cut(s)	8, 45, 82, 1379, 2017, 2344, 2541
BspFNI	CGCG	2 cut(s)	1482, 2830
BspLI	GGNNCC	9 cut(s)	24, 262, 467, 566, 1368, 1420, 1651, 2247, 2307
BspMAI	CTGCAG	1 cut(s)	915
BspOI	GCTAGC	1 cut(s)	1695
BspPI	GGATC	4 cut(s)	503, 1263, 1305, 1614
BspT104I	TTCGAA	1 cut(s)	2675
BspT107I	GGYRCC	4 cut(s)	1366, 1418, 1649, 2245
BspTNI	GGTCTC	1 cut(s)	1224
BsrI	ACTGG	1 cut(s)	566
BssECI	CCNNGG	7 cut(s)	46, 438, 543, 1881, 1943, 2019, 2625
BssMI	GATC	9 cut(s)	444, 508, 644, 742, 1255, 1297, 1409, 1606, 2373
BssNAI	GTATAC	1 cut(s)	345
BssNI	GRCGYC	2 cut(s)	141, 1367
BssT1I	CCWWGG	3 cut(s)	46, 438, 1943
Bst1107I	GTATAC	1 cut(s)	345
Bst2UI	CCWGG	1 cut(s)	2729
Bst4CI	ACNGT	7 cut(s)	841, 1403, 1445, 1587, 1759, 2126, 2839
Bst6I	CTCTTC	2 cut(s)	1287, 1635
BstACI	GRCGYC	2 cut(s)	141, 1367
BstAPI	GCANNNNNTGC	1 cut(s)	2108
BstBAI	YACGTR	2 cut(s)	686, 1325
BstBI	TTCGAA	1 cut(s)	2675
BstC8I	GCNNGC	8 cut(s)	39, 43, 637, 1012, 1693, 2395, 2603, 2793
BstDSI	CCRYGG	3 cut(s)	46, 438, 1943
BstEII	GGTNACC	1 cut(s)	2444
BstF5I	GGATG	4 cut(s)	764, 2290, 2395, 2635
BstFNI	CGCG	2 cut(s)	1482, 2830
BstH2I	RGCGCY	1 cut(s)	1370
BstHHI	GCGC	2 cut(s)	1040, 1369
BstKTI	GATC	9 cut(s)	447, 511, 647, 745, 1258, 1300, 1412, 1609, 2376
BstMBI	GATC	9 cut(s)	444, 508, 644, 742, 1255, 1297, 1409, 1606, 2373
BstMCI	CGRYCG	2 cut(s)	1761, 2208
BstMWI	GCNNNNNNNGC	7 cut(s)	941, 1016, 1222, 1736, 2093, 2108, 2602
BstNI	CCWGG	1 cut(s)	2729
BstNSI	RCATGY	1 cut(s)	878
BstPAI	GACNNNNGTC	1 cut(s)	1757
BstPI	GGTNACC	1 cut(s)	2444
BstSCI	CCNGG	2 cut(s)	1517, 2727
BstSFI	CTRYAG	2 cut(s)	911, 1725
BstSLI	GKGCMC	1 cut(s)	839
BstUI	CGCG	2 cut(s)	1482, 2830
BstV1I	GCAGC	3 cut(s)	63, 2098, 2349
BstX2I	RGATCY	6 cut(s)	444, 644, 1255, 1297, 1409, 1606
BstYI	RGATCY	6 cut(s)	444, 644, 1255, 1297, 1409, 1606
BstZ17I	GTATAC	1 cut(s)	345
Bsu36I	CCTNAGG	1 cut(s)	1744
BsuI	GTATCC	1 cut(s)	2131
BsuRI	GGCC	7 cut(s)	8, 45, 82, 1379, 2017, 2344, 2541
BtgI	CCRYGG	3 cut(s)	46, 438, 1943
BtgZI	GCGATG	1 cut(s)	1923
BtsCI	GGATG	4 cut(s)	764, 2290, 2395, 2635
BtsI	GCAGTG	1 cut(s)	337
BtsIMutI	CAGTG	6 cut(s)	337, 837, 1408, 1506, 2045, 2835
BtuMI	TCGCGA	1 cut(s)	1482
Cac8I	GCNNGC	8 cut(s)	39, 43, 637, 1012, 1693, 2395, 2603, 2793
CaiI	CAGNNNCTG	1 cut(s)	176
CfoI	GCGC	2 cut(s)	1040, 1369
Cfr13I	GGNCC	5 cut(s)	466, 1177, 2016, 2305, 2731
CseI	GACGC	2 cut(s)	149, 1656
Csp6I	GTAC	5 cut(s)	190, 250, 522, 954, 2246
CspCI	CAANNNNNGTGG	2 cut(s)	2740, 2775
CviQI	GTAC	5 cut(s)	190, 250, 522, 954, 2246
DinI	GGCGCC	1 cut(s)	1368
DpnI	GATC	9 cut(s)	446, 510, 646, 744, 1257, 1299, 1411, 1608, 2375
DpnII	GATC	9 cut(s)	444, 508, 644, 742, 1255, 1297, 1409, 1606, 2373
DraI	TTTAAA	1 cut(s)	2440
DriI	GACNNNNNGTC	1 cut(s)	2070
EaeI	YGGCCR	4 cut(s)	6, 43, 1377, 2539
Eam1104I	CTCTTC	2 cut(s)	1287, 1635
Eam1105I	GACNNNNNGTC	1 cut(s)	2070
EarI	CTCTTC	2 cut(s)	1287, 1635
Ecl136II	GAGCTC	1 cut(s)	2479
Eco130I	CCWWGG	3 cut(s)	46, 438, 1943
Eco24I	GRGCYC	2 cut(s)	2481, 2787
Eco31I	GGTCTC	1 cut(s)	1224
Eco47I	GGWCC	4 cut(s)	466, 1177, 2305, 2731
Eco53kI	GAGCTC	1 cut(s)	2479
Eco57I	CTGAAG	2 cut(s)	1166, 2331
Eco72I	CACGTG	1 cut(s)	686
Eco81I	CCTNAGG	1 cut(s)	1744
Eco88I	CYCGRG	2 cut(s)	2018, 2786
Eco91I	GGTNACC	1 cut(s)	2444
EcoICRI	GAGCTC	1 cut(s)	2479
EcoO109I	RGGNCCY	2 cut(s)	466, 1177
EcoO65I	GGTNACC	1 cut(s)	2444
EcoRI	GAATTC	2 cut(s)	416, 2351
EcoRII	CCWGG	1 cut(s)	2727
EcoT14I	CCWWGG	3 cut(s)	46, 438, 1943
EcoT22I	ATGCAT	1 cut(s)	134
EcoT38I	GRGCYC	2 cut(s)	2481, 2787
EgeI	GGCGCC	1 cut(s)	1368
EheI	GGCGCC	1 cut(s)	1368
ErhI	CCWWGG	3 cut(s)	46, 438, 1943
Esp3I	CGTCTC	2 cut(s)	1387, 1725
FalI	AAGNNNNNCTT	6 cut(s)	1908, 1940, 2164, 2196, 2329, 2361
FaqI	GGGAC	2 cut(s)	479, 1355
FauI	CCCGC	4 cut(s)	237, 911, 1990, 2563
FauNDI	CATATG	1 cut(s)	316
FblI	GTMKAC	2 cut(s)	344, 2668
Fnu4HI	GCNGC	4 cut(s)	77, 80, 2112, 2363
FokI	GGATG	4 cut(s)	751, 2297, 2402, 2642
FriOI	GRGCYC	2 cut(s)	2481, 2787
Fsp4HI	GCNGC	4 cut(s)	77, 80, 2112, 2363
FspBI	CTAG	7 cut(s)	105, 503, 936, 1101, 1106, 1452, 1692
GlaI	GCGC	2 cut(s)	1039, 1368
GluI	GCNGC	4 cut(s)	77, 80, 2112, 2363
GsuI	CTGGAG	1 cut(s)	625
HaeII	RGCGCY	1 cut(s)	1370
HaeIII	GGCC	7 cut(s)	8, 45, 82, 1379, 2017, 2344, 2541
HapII	CCGG	3 cut(s)	73, 1519, 2249
HgaI	GACGC	2 cut(s)	149, 1656
HhaI	GCGC	2 cut(s)	1040, 1369
Hin1I	GRCGYC	2 cut(s)	141, 1367
Hin6I	GCGC	2 cut(s)	1038, 1367
HinP1I	GCGC	2 cut(s)	1038, 1367
HincII	GTYRAC	1 cut(s)	1048
HindII	GTYRAC	1 cut(s)	1048
HpaI	GTTAAC	1 cut(s)	1048
HpaII	CCGG	3 cut(s)	73, 1519, 2249
HphI	GGTGA	4 cut(s)	532, 1087, 1788, 2456
Hpy166II	GTNNAC	8 cut(s)	250, 345, 733, 758, 824, 1048, 2413, 2669
Hpy8I	GTNNAC	8 cut(s)	250, 345, 733, 758, 824, 1048, 2413, 2669
HpyCH4III	ACNGT	7 cut(s)	841, 1403, 1445, 1587, 1759, 2126, 2839
HpyCH4IV	ACGT	9 cut(s)	341, 685, 760, 773, 1050, 1206, 1306, 1324, 2400
HpyF10VI	GCNNNNNNNGC	7 cut(s)	941, 1016, 1222, 1736, 2093, 2108, 2602
HpySE526I	ACGT	9 cut(s)	341, 685, 760, 773, 1050, 1206, 1306, 1324, 2400
Hsp92I	GRCGYC	2 cut(s)	141, 1367
HspAI	GCGC	2 cut(s)	1038, 1367
KasI	GGCGCC	1 cut(s)	1366
KpnI	GGTACC	1 cut(s)	2249
KspAI	GTTAAC	1 cut(s)	1048
Kzo9I	GATC	9 cut(s)	444, 508, 644, 742, 1255, 1297, 1409, 1606, 2373
LmnI	GCTCC	4 cut(s)	188, 260, 644, 1213
Lsp1109I	GCAGC	3 cut(s)	63, 2098, 2349
LweI	GCATC	3 cut(s)	773, 1573, 2380
MaeI	CTAG	7 cut(s)	105, 503, 936, 1101, 1106, 1452, 1692
MaeII	ACGT	9 cut(s)	341, 685, 760, 773, 1050, 1206, 1306, 1324, 2400
MaeIII	GTNAC	4 cut(s)	1027, 1794, 2120, 2444
MalI	GATC	9 cut(s)	446, 510, 646, 744, 1257, 1299, 1411, 1608, 2375
MboI	GATC	9 cut(s)	444, 508, 644, 742, 1255, 1297, 1409, 1606, 2373
MfeI	CAATTG	3 cut(s)	525, 2693, 2754
MflI	RGATCY	6 cut(s)	444, 644, 1255, 1297, 1409, 1606
MhlI	GDGCHC	4 cut(s)	839, 1979, 2481, 2787
MlsI	TGGCCA	3 cut(s)	8, 45, 2541
MluNI	TGGCCA	3 cut(s)	8, 45, 2541
Mly113I	GGCGCC	1 cut(s)	1367
MlyI	GAGTC	7 cut(s)	970, 1652, 1802, 1912, 2252, 2613, 2808
MmeI	TCCRAC	4 cut(s)	99, 1041, 1498, 1741
Mox20I	TGGCCA	3 cut(s)	8, 45, 2541
Mph1103I	ATGCAT	1 cut(s)	134
MroXI	GAANNNNTTC	3 cut(s)	569, 1141, 2086
MscI	TGGCCA	3 cut(s)	8, 45, 2541
MseI	TTAA	6 cut(s)	135, 1047, 1251, 1895, 2279, 2439
MslI	CAYNNNNRTG	3 cut(s)	975, 1849, 1942
Msp20I	TGGCCA	3 cut(s)	8, 45, 2541
MspA1I	CMGCKG	7 cut(s)	41, 291, 493, 725, 782, 1148, 2153
MspI	CCGG	3 cut(s)	73, 1519, 2249
MspR9I	CCNGG	2 cut(s)	1519, 2729
MunI	CAATTG	3 cut(s)	525, 2693, 2754
Mva1269I	GAATGC	3 cut(s)	134, 1536, 2795
MvaI	CCWGG	1 cut(s)	2729
MvnI	CGCG	2 cut(s)	1482, 2830
MwoI	GCNNNNNNNGC	7 cut(s)	941, 1016, 1222, 1736, 2093, 2108, 2602
NarI	GGCGCC	1 cut(s)	1367
NciI	CCSGG	1 cut(s)	1519
NcoI	CCATGG	3 cut(s)	46, 438, 1943
NdeI	CATATG	1 cut(s)	316
NdeII	GATC	9 cut(s)	444, 508, 644, 742, 1255, 1297, 1409, 1606, 2373
NheI	GCTAGC	1 cut(s)	1691
NlaIV	GGNNCC	9 cut(s)	24, 262, 467, 566, 1368, 1420, 1651, 2247, 2307
NmuCI	GTSAC	4 cut(s)	1027, 1794, 2120, 2444
NruI	TCGCGA	1 cut(s)	1482
NsiI	ATGCAT	1 cut(s)	134
NspI	RCATGY	1 cut(s)	878
NspV	TTCGAA	1 cut(s)	2675
PcsI	WCGNNNNNNNCGW	1 cut(s)	375
PctI	GAATGC	3 cut(s)	134, 1536, 2795
PdmI	GAANNNNTTC	3 cut(s)	569, 1141, 2086
PfeI	GAWTC	8 cut(s)	455, 655, 890, 1151, 1511, 1682, 2237, 2456
PflFI	GACNNNGTC	1 cut(s)	2837
PflMI	CCANNNNNTGG	1 cut(s)	2740
PkrI	GCNGC	4 cut(s)	78, 81, 2113, 2364
PleI	GAGTC	7 cut(s)	969, 1651, 1801, 1911, 2252, 2613, 2808
PluTI	GGCGCC	1 cut(s)	1370
PmaCI	CACGTG	1 cut(s)	686
PmlI	CACGTG	1 cut(s)	686
PpsI	GAGTC	7 cut(s)	969, 1651, 1801, 1911, 2252, 2613, 2808
Ppu21I	YACGTR	2 cut(s)	686, 1325
PpuMI	RGGWCCY	2 cut(s)	466, 1177
PshAI	GACNNNNGTC	1 cut(s)	1757
PshBI	ATTAAT	1 cut(s)	135
Psp124BI	GAGCTC	1 cut(s)	2481
Psp1406I	AACGTT	1 cut(s)	1306
Psp5II	RGGWCCY	2 cut(s)	466, 1177
Psp6I	CCWGG	1 cut(s)	2727
PspCI	CACGTG	1 cut(s)	686
PspEI	GGTNACC	1 cut(s)	2444
PspGI	CCWGG	1 cut(s)	2727
PspN4I	GGNNCC	9 cut(s)	24, 262, 467, 566, 1368, 1420, 1651, 2247, 2307
PspPI	GGNCC	5 cut(s)	466, 1177, 2016, 2305, 2731
PspPPI	RGGWCCY	2 cut(s)	466, 1177
PsrI	GAACNNNNNNTAC	2 cut(s)	1438, 1470
PstI	CTGCAG	1 cut(s)	915
PstNI	CAGNNNCTG	1 cut(s)	176
PsuI	RGATCY	6 cut(s)	444, 644, 1255, 1297, 1409, 1606
PsyI	GACNNNGTC	1 cut(s)	2837
PvuII	CAGCTG	5 cut(s)	41, 725, 782, 1148, 2153
RruI	TCGCGA	1 cut(s)	1482
RsaI	GTAC	5 cut(s)	191, 251, 523, 955, 2247
RsaNI	GTAC	5 cut(s)	190, 250, 522, 954, 2246
RseI	CAYNNNNRTG	3 cut(s)	975, 1849, 1942
SacI	GAGCTC	1 cut(s)	2481
SaqAI	TTAA	6 cut(s)	135, 1047, 1251, 1895, 2279, 2439
SatI	GCNGC	4 cut(s)	77, 80, 2112, 2363
Sau3AI	GATC	9 cut(s)	444, 508, 644, 742, 1255, 1297, 1409, 1606, 2373
Sau96I	GGNCC	5 cut(s)	466, 1177, 2016, 2305, 2731
SchI	GAGTC	7 cut(s)	970, 1652, 1802, 1912, 2252, 2613, 2808
ScrFI	CCNGG	2 cut(s)	1519, 2729
SduI	GDGCHC	4 cut(s)	839, 1979, 2481, 2787
SfaNI	GCATC	3 cut(s)	773, 1573, 2380
SfcI	CTRYAG	2 cut(s)	911, 1725
SfoI	GGCGCC	1 cut(s)	1368
SfuI	TTCGAA	1 cut(s)	2675
SinI	GGWCC	4 cut(s)	466, 1177, 2305, 2731
SmiMI	CAYNNNNRTG	3 cut(s)	975, 1849, 1942
SmlI	CTYRAG	2 cut(s)	301, 1196
SmoI	CTYRAG	2 cut(s)	301, 1196
SpeI	ACTAGT	1 cut(s)	1451
SspDI	GGCGCC	1 cut(s)	1366
SspMI	CTAG	7 cut(s)	105, 503, 936, 1101, 1106, 1452, 1692
SstI	GAGCTC	1 cut(s)	2481
StyD4I	CCNGG	2 cut(s)	1517, 2727
StyI	CCWWGG	3 cut(s)	46, 438, 1943
TaaI	ACNGT	7 cut(s)	841, 1403, 1445, 1587, 1759, 2126, 2839
TaiI	ACGT	9 cut(s)	344, 688, 763, 776, 1053, 1209, 1309, 1327, 2403
TaqI	TCGA	3 cut(s)	741, 1906, 2675
TaqII	GACCGA	1 cut(s)	2222
TauI	GCSGC	1 cut(s)	82
TfiI	GAWTC	8 cut(s)	455, 655, 890, 1151, 1511, 1682, 2237, 2456
Tru1I	TTAA	6 cut(s)	135, 1047, 1251, 1895, 2279, 2439
Tru9I	TTAA	6 cut(s)	135, 1047, 1251, 1895, 2279, 2439
TscAI	CASTG	6 cut(s)	337, 844, 1408, 1513, 2052, 2842
TseFI	GTSAC	4 cut(s)	1027, 1794, 2120, 2444
TseI	GCWGC	3 cut(s)	76, 2111, 2362
Tsp45I	GTSAC	4 cut(s)	1027, 1794, 2120, 2444
TspDTI	ATGAA	7 cut(s)	195, 645, 1326, 1489, 1926, 2582, 2646
TspGWI	ACGGA	3 cut(s)	422, 572, 836
TspRI	CASTG	6 cut(s)	337, 844, 1408, 1513, 2052, 2842
Tth111I	GACNNNGTC	1 cut(s)	2837
Van91I	CCANNNNNTGG	1 cut(s)	2740
VpaK11BI	GGWCC	4 cut(s)	466, 1177, 2305, 2731
VspI	ATTAAT	1 cut(s)	135
XapI	RAATTY	5 cut(s)	416, 1333, 1495, 1597, 2351
XbaI	TCTAGA	1 cut(s)	502
XceI	RCATGY	1 cut(s)	878
XmiI	GTMKAC	2 cut(s)	344, 2668
XmnI	GAANNNNTTC	3 cut(s)	569, 1141, 2086
XspI	CTAG	7 cut(s)	105, 503, 936, 1101, 1106, 1452, 1692
Zsp2I	ATGCAT	1 cut(s)	134

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.