Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0003296

Physical Location & Seq

Genomic Coordinates Reverse (-)

8962419 .. 8969241

Gene Structure 6823 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1110400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2853 bp

ATGGTGGCCGTTGATGGCGAGGAGCCAATGCAAGAGCCAGCTGGCCACGGGGAGAGAATATTGGTTTCAGTCCGGCTGCGGCCTCTGGTTGAGAAGGAGACTGCTAGAAATGATGTGTCGGATTGGGAATGCATTAATGACACAACTATCATATACAGGAACAATCTTTCAGTTTCCGAACGCTCCATGTACCCAACTGCCTACACATTTGACAGAGTATTTGGGACTGACTGCTCCACAAGGCGGGTGTATGAAGAGGGAGCCAAGAGAGTTGCTCTTTCAGTTGTCAGCGGTATAAACTCAAGTATCTTTGCATATGGACAAACAAGCAGTGGAAAGACGTATACCATGAGTGCAATTACGGACTACACTGTTGCGGATATATATGACTACATAGAGAAGCACACGGAGAGAGAATTCAATTTGAAGTTCTCTGCCCTGGAGATCTACAACGAATCTGTTAGGGACCTCCTTAGTGCAGATACCACTCCGCTTAGACTTCTAGATGATCCAGAGAGAGGTACAATTGTTGAGAAACTCACCGAGGAAACTCTAACGGACTGGAACCATTTCAAAGAACTTCTATCTGTCTGTGAAGCTCAAAGACAGATAGGGGAAACAGCCCTAAATGAAGCAAGCTCCAGATCTCATCAGATTCTCAGATTGGTCATTGAAAGTTCAGCGCGTGAGTTTCTTGGCTATGACAAATCAAGCTCCCTTACTGCTGTTGTGAACTTTGTTGATCTTGCTGGAAGTGAACGTGCGTCCCAGACGTTATCAGCTGGAGCAAGGTTGAAAGAGGGTTGCCACATAAATCGCAGTTTACTAACTCTAGGCACTGTTATCCGTAAGCTCAGCAAGGGAAGAAGTGGGCATTCCTCGTTGGGAGGAAATGCTAGAACTGCCATCATCTGTACCATGAGTCCTGCACATAGCCATGTTGAGCAATCAAGAAATACCCTCTTATTTGCGAGCTGTGCTAAAGAAGTGACAACAAGCGCACAAGTTAACGTGGTCATGTCGGATAAAGCATTGGTGAAGCATTTGCAAAAAGAATTGACTAGACTAGAGAATGAGTTGAGAGGTTCAGGACACAAAATTGTTTCAGCTGATTCTTCAACATTACTGAGAGAAAAGGACCTTCAAATTGAAAAGCTCAAAAAAGAGGTATCGGAGCTAACTCAGCAACGAGACCTTGCTCAATCTCAGGTTAAGGATCTGGTGCGAGTGCTTGGAGATGATAAACCATCAGAAGAGGATCTGGAACGTTATTACCCCAAATTGCGTGGGCAAGTTTCATGGGACTTTGAAATGAAAATGGCAGGTGCGCCTGTTCGGGCCGTCTCTCGTGGCCGAGCCAACAGTGTGAGATCTTTTGGCACCTCTCAGTATTCTGATGGAGACAGTAGGACTAGTTCAGAGGAGACTCTGTTCCAACTTCCTGACTTGGAAGAGAATTTCATGCACACTGATTCCTCCCGGCAGTTATCCGATGGCATTCCTAATTTTGTTGACGGTAATCTGCATCAAGAGGAAAGCAAAGAACAGTTTGATGGAAATTCAGAGGATTTCTGCAAGGAAGTTCGCTGCATTGAAATGGAAGAGTCTGGCACAAACAGATATATAGTGTCAAATATATCAGATTCAAGTGCTAGTAGATACCAAAACTCAAATATGTCGTCTCCTTTCGCAAACACAGCTACCTCAGGACTGACCACAGTCGAAAATGGAGATGGCACAAATCAGGAATTGGAGTCACCCCTACCTAAACAAAAAGGTTTTGTAGTTCCTTCTTCTGAAAGGACACCTCAATGGCTGCCCGAGAAAGAAATGTTTTGCCCCTCTGTTGTGATGTTGAGGAGGAGTAGAAGCTGCAAAGCAAGGCTTATGAATAATTCGTCTCCATGGTGGCTTGAGTTGGTAGAGAAGAATGAGAGCACACCTGCTATTGAGTTTGAGAAAAAATTTATAGGAAGGCCCGAGGGTTTCCAAAAGAAACTTCCTTCTCTGAACTATGGTGCTGAGACTGAAAGGCTATCAAGAAATGCTTCTCAGGCTTCTGCAAGAAGTGCTGCTATTGATGAGCGTAAAGCACAGGATACAAAATGCACAACTGATGACAAGGTAACTGAAAGCAGTACTTTAGTTGAAGGAGCAGAGGAAACGACCGATACACAATGCAATACTCAACTTGCTGATTCTGTGGTACCGGAGACGGATTCAATGCCCATACCGTCTCCAAAGGATGTAAAAGATGTTGGCTTGGACCCAATGCCCATTGATGACGAAAGCCCTTCAAGGTGGCCTTCAGAATTCAACAAGCTGCAAAGAGAGATCATTGAACTTTGGGATGCCTGCAATGTGTCATTGGTTCACAGGACTTACTTCTTCCTCCTATTTAAAGGTGACCCAAGTGATTCGATTTACATGGAGGTTGAGGTCAGGAGATTGTCCTTCCTCAAAGACACATTTTCGCGTGGAAATCAGACTCTGGAAGATGGCCAAACTCTAACACCCGCTTCAAGCATGAAGGCTTTGACTAGTGAGAGACACATGCTGAGCAAGCAAATGCGAAGGCGACTGTCTGAGGATGAAAGAAATAACCTCTACCTAAAGTGGGGTATCAGTCTACACTCGAAGCATAGGCGGTTGCAATTGGCAAACCTCCTGTGGAGTAACACACAAAACCTGGACCATATCATGGACAGTGCCACAATTGTTGCAAAGTTGGTAGGTTCAGTAGAGCCCGAGCAAGCATTCAAGGAGATGTTTGGTCTCAGGTTTGCACCGCGAGACACTCTCACCAAGCGGAAATCTCACTTTTGGTCAGAAAGTTTCAGGTCTCTTGTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0000278 GO:0000280 GO:0000775 GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005694 GO:0005856 GO:0005871 GO:0005875 GO:0005911 GO:0006928 GO:0006996 GO:0007010 GO:0007017 GO:0007018 GO:0007049 GO:0007051 GO:0007052 GO:0008150 GO:0009506 GO:0009987 GO:0015630 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022402 GO:0022607 GO:0030054 GO:0032991 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043515 GO:0044085 GO:0044422 GO:0044424 GO:0044427 GO:0044430 GO:0044446 GO:0044464 GO:0044877 GO:0048285 GO:0051225 GO:0055044 GO:0070925 GO:0071840 GO:0090307 GO:0098687 GO:0140014 GO:1902850 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF3490 Kinesin

Protein Analysis

951

Amino Acids

106.93

Weight (kDa)

5.48

Isoelectric Point (pI)

55.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Kinesin	PF00225	25 - 330	1.8e-80	Kinesin motor domain
Microtub_bd	PF16796	47 - 158	2e-20	Microtubule binding
DUF3490	PF11995	771 - 930	2.5e-76	Domain of unknown function (DUF3490)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003296)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G51150 AT3G51150 AT3G51150 AT3G51150 AT3G51150 AT5G66310 AT5G66310 AT5G66310
fragaria_vesca	FvH4_1g12070 FvH4_1g12070 FvH4_1g12070 FvH4_1g12070 FvH4_1g12070 FvH4_1g12070 FvH4_1g12070
malus_domestica	MD00G1176100.v1.1 MD02G1110400.v1.1 MD02G1135300.v1.1 MD15G1248100.v1.1
prunus_persica	Prupe.7G166000_v2.0.a1 Prupe.7G166000_v2.0.a1
pyrus_communis	pycom02g10640 pycom15g21790
rosa_chinensis	RchiOBHm_Chr2g0099691
rosa_laevigata	RLG00000016906
rosa_multiflora	Rmu_sc0004019.1_g000007
rosa_roxburghii	Rroxscaffold_2G00142830
rosa_rugosa	Rorug02G0082100 Rorug02G0082200 Rorug02G0082300 Rorug02G0082400
rosa_samantha	Rh2AG131700 Rh2BG135000 Rh2CG135900 Rh2DG136400
rosa_wichuraiana	Rw2G010220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	1313, 1951
Acc36I	ACCTGC	2 cut(s)	1313, 1951
Acc65I	GGTACC	1 cut(s)	2206
AccB1I	GGYRCC	2 cut(s)	1379, 2206
AccB7I	CCANNNNNTGG	1 cut(s)	2701
AccI	GTMKAC	2 cut(s)	344, 2629
AccII	CGCG	3 cut(s)	685, 2476, 2791
AciI	CCGC	9 cut(s)	79, 244, 291, 377, 491, 2517, 2647, 2789, 2809
AclI	AACGTT	1 cut(s)	1267
AclWI	GGATC	3 cut(s)	503, 1224, 1266
AcoI	YGGCCR	4 cut(s)	6, 43, 1351, 2500
AcsI	RAATTY	5 cut(s)	416, 1456, 1558, 1964, 2312
AcuI	CTGAAG	1 cut(s)	2292
AfaI	GTAC	5 cut(s)	191, 523, 916, 2140, 2208
AfiI	CCNNNNNNNGG	4 cut(s)	243, 518, 2617, 2701
AhlI	ACTAGT	2 cut(s)	1412, 2540
AjnI	CCWGG	2 cut(s)	438, 2688
AjuI	GAANNNNNNNTTGG	2 cut(s)	1983, 2015
AloI	GAACNNNNNNTCC	2 cut(s)	1415, 1447
Alw21I	GWGCWC	1 cut(s)	1940
AlwI	GGATC	3 cut(s)	503, 1224, 1266
AlwNI	CAGNNNCTG	1 cut(s)	2491
Ama87I	CYCGRG	3 cut(s)	1820, 1979, 2747
AoxI	GGCC	8 cut(s)	6, 43, 80, 1338, 1351, 1976, 2303, 2500
ApeKI	GCWGC	6 cut(s)	76, 1587, 1816, 1872, 2072, 2323
ApoI	RAATTY	5 cut(s)	416, 1456, 1558, 1964, 2312
ArsI	GACNNNNNNTTYG	4 cut(s)	203, 235, 1664, 1696
AseI	ATTAAT	1 cut(s)	135
Asp700I	GAANNNNTTC	2 cut(s)	569, 2047
Asp718I	GGTACC	1 cut(s)	2206
AspLEI	GCGC	3 cut(s)	685, 1001, 1330
AspS9I	GGNCC	6 cut(s)	466, 1138, 1338, 1977, 2266, 2692
AsuC2I	CCSGG	1 cut(s)	1480
AsuHPI	GGTGA	5 cut(s)	532, 1048, 1749, 2417, 2794
AvaI	CYCGRG	3 cut(s)	1820, 1979, 2747
AvaII	GGWCC	4 cut(s)	466, 1138, 2266, 2692
AxyI	CCTNAGG	1 cut(s)	1705
BaeI	ACNNNNGTAYC	4 cut(s)	289, 322, 513, 546
BalI	TGGCCA	2 cut(s)	45, 2502
BanI	GGYRCC	2 cut(s)	1379, 2206
BanII	GRGCYC	1 cut(s)	2748
BauI	CACGAG	1 cut(s)	1347
Bbv12I	GWGCWC	1 cut(s)	1940
BbvI	GCAGC	6 cut(s)	63, 1574, 1803, 1859, 2059, 2310
BccI	CCATC	8 cut(s)	8, 914, 1255, 1391, 1487, 1547, 1727, 2492
BceAI	ACGGC	1 cut(s)	1325
BciT130I	CCWGG	2 cut(s)	440, 2690
BciVI	GTATCC	1 cut(s)	2092
BcnI	CCSGG	1 cut(s)	1480
BcuI	ACTAGT	2 cut(s)	1412, 2540
BfaI	CTAG	9 cut(s)	105, 503, 833, 897, 1062, 1067, 1413, 1653, 2541
BfuAI	ACCTGC	2 cut(s)	1313, 1951
BfuI	GTATCC	1 cut(s)	2092
BglII	AGATCT	3 cut(s)	444, 644, 1370
BisI	GCNGC	7 cut(s)	77, 80, 1588, 1817, 1873, 2073, 2324
BlpI	GCTNAGC	2 cut(s)	854, 2558
BlsI	GCNGC	7 cut(s)	78, 81, 1589, 1818, 1874, 2074, 2325
BmcAI	AGTACT	1 cut(s)	2140
Bme1390I	CCNGG	3 cut(s)	440, 1480, 2690
Bme18I	GGWCC	4 cut(s)	466, 1138, 2266, 2692
BmeT110I	CYCGRG	3 cut(s)	1820, 1979, 2747
BmgT120I	GGNCC	6 cut(s)	466, 1138, 1338, 1977, 2266, 2692
BmiI	GGNNCC	7 cut(s)	24, 262, 467, 566, 1381, 2208, 2268
BmrFI	CCNGG	3 cut(s)	440, 1480, 2690
BmsI	GCATC	2 cut(s)	1534, 2341
BoxI	GACNNNNGTC	1 cut(s)	1718
BplI	GAGNNNNNCTC	4 cut(s)	259, 291, 2784, 2816
BpmI	CTGGAG	3 cut(s)	461, 625, 804
Bpu1102I	GCTNAGC	2 cut(s)	854, 2558
BpuEI	CTTGAG	2 cut(s)	286, 1934
BpuMI	CCSGG	1 cut(s)	1480
BsaI	GGTCTC	3 cut(s)	1185, 2780, 2847
BsaJI	CCNNGG	5 cut(s)	46, 438, 543, 1904, 1980
BsaWI	WCCGGW	1 cut(s)	2209
BsaXI	ACNNNNNCTCC	2 cut(s)	1415, 1445
Bsc4I	CCNNNNNNNGG	4 cut(s)	243, 518, 2617, 2701
Bse1I	ACTGG	1 cut(s)	566
Bse21I	CCTNAGG	1 cut(s)	1705
Bse3DI	GCAATG	1 cut(s)	2365
BseBI	CCWGG	2 cut(s)	440, 2690
BseDI	CCNNGG	5 cut(s)	46, 438, 543, 1904, 1980
BseGI	GGATG	3 cut(s)	2251, 2356, 2596
BseLI	CCNNNNNNNGG	4 cut(s)	243, 518, 2617, 2701
BseMI	GCAATG	1 cut(s)	2365
BseNI	ACTGG	1 cut(s)	566
BseRI	GAGGAG	4 cut(s)	35, 1436, 1873, 1876
BseXI	GCAGC	6 cut(s)	63, 1574, 1803, 1859, 2059, 2310
BsgI	GTGCAG	2 cut(s)	498, 912
Bsh1236I	CGCG	3 cut(s)	685, 2476, 2791
Bsh1285I	CGRYCG	1 cut(s)	2169
BshFI	GGCC	8 cut(s)	8, 45, 82, 1340, 1353, 1978, 2305, 2502
BshNI	GGYRCC	2 cut(s)	1379, 2206
BsiEI	CGRYCG	1 cut(s)	2169
BsiHKAI	GWGCWC	1 cut(s)	1940
BsiHKCI	CYCGRG	3 cut(s)	1820, 1979, 2747
BsiSI	CCGG	3 cut(s)	73, 1480, 2210
BslFI	GGGAC	4 cut(s)	238, 479, 751, 1316
BslI	CCNNNNNNNGG	4 cut(s)	243, 518, 2617, 2701
BsmBI	CGTCTC	5 cut(s)	1348, 1686, 1905, 2207, 2241
BsmFI	GGGAC	4 cut(s)	238, 479, 751, 1316
BsmI	GAATGC	4 cut(s)	134, 874, 1497, 2756
BsnI	GGCC	8 cut(s)	8, 45, 82, 1340, 1353, 1978, 2305, 2502
Bso31I	GGTCTC	3 cut(s)	1185, 2780, 2847
BsoBI	CYCGRG	3 cut(s)	1820, 1979, 2747
Bsp1286I	GDGCHC	2 cut(s)	1940, 2748
Bsp143I	GATC	8 cut(s)	444, 508, 644, 742, 1216, 1258, 1370, 2334
Bsp1720I	GCTNAGC	2 cut(s)	854, 2558
Bsp19I	CCATGG	1 cut(s)	1904
BspACI	CCGC	9 cut(s)	79, 244, 291, 377, 491, 2517, 2647, 2789, 2809
BspANI	GGCC	8 cut(s)	8, 45, 82, 1340, 1353, 1978, 2305, 2502
BspFNI	CGCG	3 cut(s)	685, 2476, 2791
BspLI	GGNNCC	7 cut(s)	24, 262, 467, 566, 1381, 2208, 2268
BspMI	ACCTGC	2 cut(s)	1313, 1951
BspPI	GGATC	3 cut(s)	503, 1224, 1266
BspT107I	GGYRCC	2 cut(s)	1379, 2206
BspTNI	GGTCTC	3 cut(s)	1185, 2780, 2847
BsrDI	GCAATG	1 cut(s)	2365
BsrI	ACTGG	1 cut(s)	566
BssECI	CCNNGG	5 cut(s)	46, 438, 543, 1904, 1980
BssMI	GATC	8 cut(s)	444, 508, 644, 742, 1216, 1258, 1370, 2334
BssNAI	GTATAC	1 cut(s)	345
BssSI	CACGAG	1 cut(s)	1347
BssT1I	CCWWGG	1 cut(s)	1904
Bst1107I	GTATAC	1 cut(s)	345
Bst2BI	CACGAG	1 cut(s)	1347
Bst2UI	CCWGG	2 cut(s)	440, 2690
Bst6I	CTCTTC	4 cut(s)	249, 1248, 1446, 1596
BstAPI	GCANNNNNTGC	1 cut(s)	2069
BstC8I	GCNNGC	7 cut(s)	39, 43, 637, 973, 2356, 2564, 2754
BstDSI	CCRYGG	2 cut(s)	46, 1904
BstEII	GGTNACC	1 cut(s)	2405
BstF5I	GGATG	3 cut(s)	2251, 2356, 2596
BstFNI	CGCG	3 cut(s)	685, 2476, 2791
BstHHI	GCGC	3 cut(s)	685, 1001, 1330
BstKTI	GATC	8 cut(s)	447, 511, 647, 745, 1219, 1261, 1373, 2337
BstMBI	GATC	8 cut(s)	444, 508, 644, 742, 1216, 1258, 1370, 2334
BstMCI	CGRYCG	1 cut(s)	2169
BstMWI	GCNNNNNNNGC	7 cut(s)	902, 977, 1183, 1697, 2054, 2069, 2563
BstNI	CCWGG	2 cut(s)	440, 2690
BstNSI	RCATGY	1 cut(s)	2557
BstPAI	GACNNNNGTC	1 cut(s)	1718
BstPI	GGTNACC	1 cut(s)	2405
BstSCI	CCNGG	3 cut(s)	438, 1478, 2688
BstUI	CGCG	3 cut(s)	685, 2476, 2791
BstV1I	GCAGC	6 cut(s)	63, 1574, 1803, 1859, 2059, 2310
BstX2I	RGATCY	5 cut(s)	444, 644, 1216, 1258, 1370
BstYI	RGATCY	5 cut(s)	444, 644, 1216, 1258, 1370
BstZ17I	GTATAC	1 cut(s)	345
Bsu36I	CCTNAGG	1 cut(s)	1705
BsuI	GTATCC	1 cut(s)	2092
BsuRI	GGCC	8 cut(s)	8, 45, 82, 1340, 1353, 1978, 2305, 2502
BtgI	CCRYGG	2 cut(s)	46, 1904
BtsCI	GGATG	3 cut(s)	2251, 2356, 2596
BtsI	GCAGTG	1 cut(s)	337
BtsIMutI	CAGTG	6 cut(s)	337, 369, 837, 1369, 1467, 2713
BveI	ACCTGC	2 cut(s)	1313, 1951
Cac8I	GCNNGC	7 cut(s)	39, 43, 637, 973, 2356, 2564, 2754
CaiI	CAGNNNCTG	1 cut(s)	2491
CfoI	GCGC	3 cut(s)	685, 1001, 1330
Cfr13I	GGNCC	6 cut(s)	466, 1138, 1338, 1977, 2266, 2692
CseI	GACGC	1 cut(s)	753
Csp6I	GTAC	5 cut(s)	190, 522, 915, 2139, 2207
CspCI	CAANNNNNGTGG	4 cut(s)	1267, 1302, 2701, 2736
CviQI	GTAC	5 cut(s)	190, 522, 915, 2139, 2207
DpnI	GATC	8 cut(s)	446, 510, 646, 744, 1218, 1260, 1372, 2336
DpnII	GATC	8 cut(s)	444, 508, 644, 742, 1216, 1258, 1370, 2334
DraI	TTTAAA	1 cut(s)	2401
EaeI	YGGCCR	4 cut(s)	6, 43, 1351, 2500
Eam1104I	CTCTTC	4 cut(s)	249, 1248, 1446, 1596
EarI	CTCTTC	4 cut(s)	249, 1248, 1446, 1596
Eco130I	CCWWGG	1 cut(s)	1904
Eco24I	GRGCYC	1 cut(s)	2748
Eco31I	GGTCTC	3 cut(s)	1185, 2780, 2847
Eco47I	GGWCC	4 cut(s)	466, 1138, 2266, 2692
Eco57I	CTGAAG	1 cut(s)	2292
Eco81I	CCTNAGG	1 cut(s)	1705
Eco88I	CYCGRG	3 cut(s)	1820, 1979, 2747
Eco91I	GGTNACC	1 cut(s)	2405
EcoO109I	RGGNCCY	2 cut(s)	466, 1138
EcoO65I	GGTNACC	1 cut(s)	2405
EcoRI	GAATTC	2 cut(s)	416, 2312
EcoRII	CCWGG	2 cut(s)	438, 2688
EcoT14I	CCWWGG	1 cut(s)	1904
EcoT22I	ATGCAT	1 cut(s)	134
EcoT38I	GRGCYC	1 cut(s)	2748
ErhI	CCWWGG	1 cut(s)	1904
Esp3I	CGTCTC	5 cut(s)	1348, 1686, 1905, 2207, 2241
FalI	AAGNNNNNCTT	8 cut(s)	1869, 1901, 2032, 2064, 2125, 2157, 2290, 2322
FaqI	GGGAC	4 cut(s)	238, 479, 751, 1316
FauI	CCCGC	2 cut(s)	237, 2524
FauNDI	CATATG	1 cut(s)	316
FblI	GTMKAC	2 cut(s)	344, 2629
Fnu4HI	GCNGC	7 cut(s)	77, 80, 1588, 1817, 1873, 2073, 2324
FokI	GGATG	3 cut(s)	2258, 2363, 2603
FriOI	GRGCYC	1 cut(s)	2748
Fsp4HI	GCNGC	7 cut(s)	77, 80, 1588, 1817, 1873, 2073, 2324
FspBI	CTAG	9 cut(s)	105, 503, 833, 897, 1062, 1067, 1413, 1653, 2541
GlaI	GCGC	3 cut(s)	684, 1000, 1329
GluI	GCNGC	7 cut(s)	77, 80, 1588, 1817, 1873, 2073, 2324
GsuI	CTGGAG	3 cut(s)	461, 625, 804
HaeIII	GGCC	8 cut(s)	8, 45, 82, 1340, 1353, 1978, 2305, 2502
HapII	CCGG	3 cut(s)	73, 1480, 2210
HgaI	GACGC	1 cut(s)	753
HhaI	GCGC	3 cut(s)	685, 1001, 1330
Hin6I	GCGC	3 cut(s)	683, 999, 1328
HinP1I	GCGC	3 cut(s)	683, 999, 1328
HincII	GTYRAC	2 cut(s)	1009, 1513
HindII	GTYRAC	2 cut(s)	1009, 1513
HpaI	GTTAAC	1 cut(s)	1009
HpaII	CCGG	3 cut(s)	73, 1480, 2210
HphI	GGTGA	5 cut(s)	532, 1048, 1749, 2417, 2794
Hpy166II	GTNNAC	8 cut(s)	345, 733, 758, 824, 1009, 1513, 2374, 2630
Hpy8I	GTNNAC	8 cut(s)	345, 733, 758, 824, 1009, 1513, 2374, 2630
HpyCH4IV	ACGT	5 cut(s)	341, 760, 773, 1011, 1267
HpyF10VI	GCNNNNNNNGC	7 cut(s)	902, 977, 1183, 1697, 2054, 2069, 2563
HpySE526I	ACGT	5 cut(s)	341, 760, 773, 1011, 1267
HspAI	GCGC	3 cut(s)	683, 999, 1328
KpnI	GGTACC	1 cut(s)	2210
KspAI	GTTAAC	1 cut(s)	1009
Kzo9I	GATC	8 cut(s)	444, 508, 644, 742, 1216, 1258, 1370, 2334
LmnI	GCTCC	9 cut(s)	22, 188, 239, 260, 644, 719, 785, 1174, 2153
Lsp1109I	GCAGC	6 cut(s)	63, 1574, 1803, 1859, 2059, 2310
LweI	GCATC	2 cut(s)	1534, 2341
MaeI	CTAG	9 cut(s)	105, 503, 833, 897, 1062, 1067, 1413, 1653, 2541
MaeII	ACGT	5 cut(s)	341, 760, 773, 1011, 1267
MaeIII	GTNAC	5 cut(s)	988, 1755, 2125, 2405, 2675
MalI	GATC	8 cut(s)	446, 510, 646, 744, 1218, 1260, 1372, 2336
MboI	GATC	8 cut(s)	444, 508, 644, 742, 1216, 1258, 1370, 2334
MfeI	CAATTG	3 cut(s)	525, 2654, 2715
MflI	RGATCY	5 cut(s)	444, 644, 1216, 1258, 1370
MhlI	GDGCHC	2 cut(s)	1940, 2748
MlsI	TGGCCA	2 cut(s)	45, 2502
MluNI	TGGCCA	2 cut(s)	45, 2502
MlyI	GAGTC	5 cut(s)	931, 1420, 1613, 1763, 2482
MmeI	TCCRAC	3 cut(s)	99, 1002, 1459
Mox20I	TGGCCA	2 cut(s)	45, 2502
Mph1103I	ATGCAT	1 cut(s)	134
MroXI	GAANNNNTTC	2 cut(s)	569, 2047
MscI	TGGCCA	2 cut(s)	45, 2502
MseI	TTAA	4 cut(s)	135, 1008, 1212, 2400
MslI	CAYNNNNRTG	3 cut(s)	936, 1595, 1810
Msp20I	TGGCCA	2 cut(s)	45, 2502
MspA1I	CMGCKG	4 cut(s)	41, 291, 782, 1109
MspI	CCGG	3 cut(s)	73, 1480, 2210
MspR9I	CCNGG	3 cut(s)	440, 1480, 2690
MunI	CAATTG	3 cut(s)	525, 2654, 2715
Mva1269I	GAATGC	4 cut(s)	134, 874, 1497, 2756
MvaI	CCWGG	2 cut(s)	440, 2690
MvnI	CGCG	3 cut(s)	685, 2476, 2791
MwoI	GCNNNNNNNGC	7 cut(s)	902, 977, 1183, 1697, 2054, 2069, 2563
NciI	CCSGG	1 cut(s)	1480
NcoI	CCATGG	1 cut(s)	1904
NdeI	CATATG	1 cut(s)	316
NdeII	GATC	8 cut(s)	444, 508, 644, 742, 1216, 1258, 1370, 2334
NlaIV	GGNNCC	7 cut(s)	24, 262, 467, 566, 1381, 2208, 2268
NmeAIII	GCCGAG	1 cut(s)	1379
NmuCI	GTSAC	3 cut(s)	988, 1755, 2405
NsiI	ATGCAT	1 cut(s)	134
NspI	RCATGY	1 cut(s)	2557
PaqCI	CACCTGC	2 cut(s)	1313, 1951
PctI	GAATGC	4 cut(s)	134, 874, 1497, 2756
PdmI	GAANNNNTTC	2 cut(s)	569, 2047
PfeI	GAWTC	8 cut(s)	455, 655, 1112, 1472, 1643, 2198, 2219, 2417
PflMI	CCANNNNNTGG	1 cut(s)	2701
PkrI	GCNGC	7 cut(s)	78, 81, 1589, 1818, 1874, 2074, 2325
PleI	GAGTC	5 cut(s)	930, 1420, 1612, 1762, 2482
PpsI	GAGTC	5 cut(s)	930, 1420, 1612, 1762, 2482
PpuMI	RGGWCCY	2 cut(s)	466, 1138
PshAI	GACNNNNGTC	1 cut(s)	1718
PshBI	ATTAAT	1 cut(s)	135
Psp1406I	AACGTT	1 cut(s)	1267
Psp5II	RGGWCCY	2 cut(s)	466, 1138
Psp6I	CCWGG	2 cut(s)	438, 2688
PspEI	GGTNACC	1 cut(s)	2405
PspGI	CCWGG	2 cut(s)	438, 2688
PspN4I	GGNNCC	7 cut(s)	24, 262, 467, 566, 1381, 2208, 2268
PspPI	GGNCC	6 cut(s)	466, 1138, 1338, 1977, 2266, 2692
PspPPI	RGGWCCY	2 cut(s)	466, 1138
PsrI	GAACNNNNNNTAC	2 cut(s)	1399, 1431
PstNI	CAGNNNCTG	1 cut(s)	2491
PsuI	RGATCY	5 cut(s)	444, 644, 1216, 1258, 1370
PvuII	CAGCTG	3 cut(s)	41, 782, 1109
RsaI	GTAC	5 cut(s)	191, 523, 916, 2140, 2208
RsaNI	GTAC	5 cut(s)	190, 522, 915, 2139, 2207
RseI	CAYNNNNRTG	3 cut(s)	936, 1595, 1810
SaqAI	TTAA	4 cut(s)	135, 1008, 1212, 2400
SatI	GCNGC	7 cut(s)	77, 80, 1588, 1817, 1873, 2073, 2324
Sau3AI	GATC	8 cut(s)	444, 508, 644, 742, 1216, 1258, 1370, 2334
Sau96I	GGNCC	6 cut(s)	466, 1138, 1338, 1977, 2266, 2692
ScaI	AGTACT	1 cut(s)	2140
SchI	GAGTC	5 cut(s)	931, 1420, 1613, 1763, 2482
ScrFI	CCNGG	3 cut(s)	440, 1480, 2690
SduI	GDGCHC	2 cut(s)	1940, 2748
SfaNI	GCATC	2 cut(s)	1534, 2341
SinI	GGWCC	4 cut(s)	466, 1138, 2266, 2692
SmiMI	CAYNNNNRTG	3 cut(s)	936, 1595, 1810
SmlI	CTYRAG	2 cut(s)	301, 1913
SmoI	CTYRAG	2 cut(s)	301, 1913
SpeI	ACTAGT	2 cut(s)	1412, 2540
SsiI	CCGC	9 cut(s)	79, 244, 291, 377, 491, 2517, 2647, 2789, 2809
SspI	AATATT	1 cut(s)	60
SspMI	CTAG	9 cut(s)	105, 503, 833, 897, 1062, 1067, 1413, 1653, 2541
StyD4I	CCNGG	3 cut(s)	438, 1478, 2688
StyI	CCWWGG	1 cut(s)	1904
TaiI	ACGT	5 cut(s)	344, 763, 776, 1014, 1270
TaqI	TCGA	3 cut(s)	1722, 2420, 2636
TaqII	GACCGA	1 cut(s)	2183
TatI	WGTACW	1 cut(s)	2138
TauI	GCSGC	1 cut(s)	82
TfiI	GAWTC	8 cut(s)	455, 655, 1112, 1472, 1643, 2198, 2219, 2417
Tru1I	TTAA	4 cut(s)	135, 1008, 1212, 2400
Tru9I	TTAA	4 cut(s)	135, 1008, 1212, 2400
TscAI	CASTG	6 cut(s)	337, 376, 844, 1369, 1474, 2713
TseFI	GTSAC	3 cut(s)	988, 1755, 2405
TseI	GCWGC	6 cut(s)	76, 1587, 1816, 1872, 2072, 2323
Tsp45I	GTSAC	3 cut(s)	988, 1755, 2405
TspDTI	ATGAA	8 cut(s)	267, 645, 1287, 1328, 1450, 1904, 2543, 2607
TspGWI	ACGGA	5 cut(s)	377, 422, 572, 836, 2231
TspRI	CASTG	6 cut(s)	337, 376, 844, 1369, 1474, 2713
Van91I	CCANNNNNTGG	1 cut(s)	2701
VpaK11BI	GGWCC	4 cut(s)	466, 1138, 2266, 2692
VspI	ATTAAT	1 cut(s)	135
XapI	RAATTY	5 cut(s)	416, 1456, 1558, 1964, 2312
XbaI	TCTAGA	1 cut(s)	502
XceI	RCATGY	1 cut(s)	2557
XmiI	GTMKAC	2 cut(s)	344, 2629
XmnI	GAANNNNTTC	2 cut(s)	569, 2047
XspI	CTAG	9 cut(s)	105, 503, 833, 897, 1062, 1067, 1413, 1653, 2541
ZrmI	AGTACT	1 cut(s)	2140
Zsp2I	ATGCAT	1 cut(s)	134

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.