AT5G67385

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007199
Physical Location & Seq
Forward (+)
26883663 .. 26887430
3768 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G67385.5

Sequence Viewer

Length: 1815 bp
ATGTCAGCAAAGAAGAAAGATCTTTTGTCCTCAGCTATGAAAAGAACCAGTGAATGGATTTCTTCTCAAGAAGTATCTAGTGATGTCACCGTTCATGTAGGAGAAGCTTCGTTTTCACTGCACAAGTTTCCACTCATGTCAAAATGTGGGTTTATCAAGAAACTTGTGTCGGAATCAAGCAAAGATTCAGATTCCACTGTTATCAAGATACCAGATATTCCAGGAGGCTCTGAAGCATTTGAGCTAGCGGCTAAGTTCTGCTACGGCATTAACTTTGATATGAGCACAGAGAACATTGCCATGTTGCGATGTGCAGCGGAGTATCTAGAGATGACAGAGGAACACTCTGTGGAAAATCTCGTTGTAAGAGCCGAAGCTTACTTGAATGAAGTAGCTCTCAAGAGCTTATCGAGTTCGATCACAGTCTTGCACAAATCAGAGAAGTTGTTGCCCATTGCTGAAAGAGTCAAACTTGTAAGCCGTTGCATCGATGCAATTGCTTATATGACTTGTCAAGAGAGCCATTTTTGCAGTCCTTCTTCAAGTAATAGCGGTAACAATGAGGTTGTGGTTCAGCAGCAGAGCAAGCAGCCTGTTGTTGATTGGTGGGCTGAAGACTTAACGGTTCTTAGGATTGATTCGTTTCAACGTGTCCTGATCGCGATGATGGCTAGAGGGTTTAAGCAGTATGGACTTGGTCCAGTGCTTATGCTCTATGCTCAGAAATCTCTTCGAGGGTTGGAAATTTTCGGGAAAGGAATGAAGAAGATTGAGCCAAAACAAGAACACGAGAAAAGAGTGATTCTTGAAACAATCGTAAGCCTTCTTCCTCGAGAGAAAAATGCAATGTCGGTTAGCTTTCTCTCTATGCTTCTACGCGCAGCAATATTCCTCGAAACAACGGTTGCTTGTAGACTTGACTTGGAAAACAGAATGGGATTACAATTAGGACAAGCCGTGCTCGATGATCTCTTGATTCCTTCTTATTCATTCACTGGAGATCACTCCATGTTTGATACTGACACTGTTCAACGCATTCTCATGAATTATCTCGAGTTTGAAGTCGAAGGAGTTCGCTTAAGCAATAATGGTGTTGATCTTGCTGGTGATATGGAACGTGTTGGTAAACTTTTGGAGAATTATATGGCTGAAATTGCTTCTGATAGAAATGTGAGCTTGCAGAAATTCATCGGTTTAGCTGAACTTATCCCCGAACAGTCTAGGGTTACTGAAGATGGTATGTATCGAGCCGTCGACATCTACCTTAAGGCGCATCCCAATATGAGTGATGTAGAGAGGAAGAAAGTGTGCAGCTTAATGGACTGCCAAAAACTATCACGAGAAGCCTGCGCTCATGCAGCTCAGAATGATCGTCTTCCTGTTCAGACCATTGTTCAAGTCCTTTACTACGAGCAACAACGTCTACGAGGAGAAGTCACTAACGATTCAGATTCTCCAGCCCCACCACCACCACAACCAGCAGCTGTTCTTCCTCCCAAACTTAGCTCCTACACCGACGAACTCTCCAAGCTTAAACGCGAAAATCAGGACTTGAAGCTCGAACTTCTCAAAATGAAAATGAAGCTAAAAGAGTTTGAGAAAGAGAGTGAGAAGAAAACATCATCATCAACCATCAGCACGAATCCAAGTTCACCAATCTCAACTGCTTCCACGGGTAAGCCTCCATTGCCAAGAAAGTCATTCATAAACTCTGTTTCCAAGAAACTCGGTAAGCTAAACCCTTTTAGCATCACACCATACAACGGTAGAGGCAGAACCAAACCACCAAAAGATCGGAGACACTCTATTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0042742 GO:0043207 GO:0050896 GO:0051704 GO:0051707 GO:0098542
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

604

Amino Acids

67.56

Weight (kDa)

8.16

Isoelectric Point (pI)

43.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 156 - 405 7.2e-88 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 54
AccI GTMKAC 3 cut(s) 913, 1254, 1423
AccII CGCG 3 cut(s) 662, 879, 1539
AciI CCGC 3 cut(s) 248, 317, 552
AcsI RAATTY 2 cut(s) 744, 1184
AcuI CTGAAG 3 cut(s) 252, 633, 1251
AdeI CACNNNGTG 1 cut(s) 349
AfiI CCNNNNNNNGG 2 cut(s) 54, 1763
AflII CTTAAG 2 cut(s) 1078, 1265
AflIII ACRYGT 2 cut(s) 649, 1117
AgsI TTSAA 8 cut(s) 385, 543, 647, 809, 1031, 1061, 1397, 1555
AjnI CCWGG 1 cut(s) 220
Alw21I GWGCWC 2 cut(s) 287, 963
Alw26I GTCTC 1 cut(s) 1792
AlwNI CAGNNNCTG 1 cut(s) 1484
Ama87I CYCGRG 2 cut(s) 831, 1052
ApeKI GCWGC 7 cut(s) 314, 577, 589, 881, 1311, 1358, 1481
ApoI RAATTY 2 cut(s) 744, 1184
Asp700I GAANNNNTTC 1 cut(s) 1071
AspLEI GCGC 3 cut(s) 881, 1273, 1352
AspS9I GGNCC 1 cut(s) 698
AsuHPI GGTGA 3 cut(s) 79, 1118, 1644
AsuNHI GCTAGC 1 cut(s) 244
AvaI CYCGRG 2 cut(s) 831, 1052
AvaII GGWCC 1 cut(s) 698
BarI GAAGNNNNNNTAC 2 cut(s) 810, 842
BauI CACGAG 2 cut(s) 788, 1338
BbsI GAAGAC 2 cut(s) 621, 1367
Bbv12I GWGCWC 2 cut(s) 287, 963
BbvCI CCTCAGC 1 cut(s) 31
BbvI GCAGC 7 cut(s) 326, 589, 601, 893, 1323, 1370, 1493
BccI CCATC 3 cut(s) 661, 1229, 1640
BceAI ACGGC 4 cut(s) 280, 465, 941, 1235
BcgI CGANNNNNNTGC 4 cut(s) 479, 513, 1329, 1363
BciT130I CCWGG 1 cut(s) 222
BcoDI GTCTC 1 cut(s) 1792
BfaI CTAG 5 cut(s) 78, 245, 326, 672, 1221
BfrI CTTAAG 2 cut(s) 1078, 1265
BglII AGATCT 1 cut(s) 19
BisI GCNGC 8 cut(s) 249, 315, 578, 590, 882, 1312, 1359, 1482
BlsI GCNGC 8 cut(s) 250, 316, 579, 591, 883, 1313, 1360, 1483
Bme1390I CCNGG 1 cut(s) 222
Bme18I GGWCC 1 cut(s) 698
BmeT110I CYCGRG 2 cut(s) 831, 1052
BmgT120I GGNCC 1 cut(s) 698
BmrFI CCNGG 1 cut(s) 222
BmsI GCATC 4 cut(s) 481, 495, 1282, 1758
BmtI GCTAGC 1 cut(s) 248
BpiI GAAGAC 2 cut(s) 621, 1367
BplI GAGNNNNNCTC 2 cut(s) 329, 361
BpmI CTGGAG 2 cut(s) 1017, 1440
Bpu10I CCTNAGC 1 cut(s) 31
BpuEI CTTGAG 2 cut(s) 51, 383
Bsa29I ATCGAT 1 cut(s) 489
BsaJI CCNNGG 1 cut(s) 1671
BsaXI ACNNNNNCTCC 2 cut(s) 1508, 1538
Bsc4I CCNNNNNNNGG 2 cut(s) 54, 1763
Bse1I ACTGG 3 cut(s) 48, 701, 1000
Bse3DI GCAATG 4 cut(s) 294, 453, 852, 1685
BseBI CCWGG 1 cut(s) 222
BseCI ATCGAT 1 cut(s) 489
BseDI CCNNGG 1 cut(s) 1671
BseGI GGATG 1 cut(s) 1273
BseLI CCNNNNNNNGG 2 cut(s) 54, 1763
BseMI GCAATG 4 cut(s) 294, 453, 852, 1685
BseMII CTCAG 3 cut(s) 45, 734, 1376
BseNI ACTGG 3 cut(s) 48, 701, 1000
BseRI GAGGAG 1 cut(s) 1443
BseXI GCAGC 7 cut(s) 326, 589, 601, 893, 1323, 1370, 1493
BsgI GTGCAG 3 cut(s) 104, 333, 1330
Bsh1236I CGCG 3 cut(s) 662, 879, 1539
BshVI ATCGAT 1 cut(s) 489
BsiHKAI GWGCWC 2 cut(s) 287, 963
BsiHKCI CYCGRG 2 cut(s) 831, 1052
BslI CCNNNNNNNGG 2 cut(s) 54, 1763
BsmAI GTCTC 1 cut(s) 1792
BsmI GAATGC 1 cut(s) 1035
BsoBI CYCGRG 2 cut(s) 831, 1052
Bsp1286I GDGCHC 2 cut(s) 287, 963
Bsp143I GATC 8 cut(s) 19, 417, 657, 967, 1000, 1096, 1369, 1792
Bsp68I TCGCGA 1 cut(s) 662
BspACI CCGC 3 cut(s) 248, 317, 552
BspCNI CTCAG 3 cut(s) 44, 733, 1375
BspDI ATCGAT 1 cut(s) 489
BspFNI CGCG 3 cut(s) 662, 879, 1539
BspHI TCATGA 1 cut(s) 1041
BspOI GCTAGC 1 cut(s) 248
BspTI CTTAAG 2 cut(s) 1078, 1265
BsrDI GCAATG 4 cut(s) 294, 453, 852, 1685
BsrI ACTGG 3 cut(s) 48, 701, 1000
BssECI CCNNGG 1 cut(s) 1671
BssMI GATC 8 cut(s) 19, 417, 657, 967, 1000, 1096, 1369, 1792
BssSI CACGAG 2 cut(s) 788, 1338
Bst2BI CACGAG 2 cut(s) 788, 1338
Bst2UI CCWGG 1 cut(s) 222
Bst4CI ACNGT 8 cut(s) 91, 199, 424, 625, 904, 1027, 1218, 1766
Bst6I CTCTTC 1 cut(s) 735
BstAFI CTTAAG 2 cut(s) 1078, 1265
BstC8I GCNNGC 4 cut(s) 246, 587, 1178, 1348
BstDEI CTNAG 6 cut(s) 31, 252, 629, 720, 1362, 1502
BstDSI CCRYGG 1 cut(s) 1671
BstF5I GGATG 1 cut(s) 1273
BstFNI CGCG 3 cut(s) 662, 879, 1539
BstHHI GCGC 3 cut(s) 881, 1273, 1352
BstKTI GATC 8 cut(s) 22, 420, 660, 970, 1003, 1099, 1372, 1795
BstMAI GTCTC 1 cut(s) 1792
BstMBI GATC 8 cut(s) 19, 417, 657, 967, 1000, 1096, 1369, 1792
BstMWI GCNNNNNNNGC 6 cut(s) 528, 586, 668, 1154, 1358, 1687
BstNI CCWGG 1 cut(s) 222
BstSCI CCNGG 1 cut(s) 220
BstUI CGCG 3 cut(s) 662, 879, 1539
BstV1I GCAGC 7 cut(s) 326, 589, 601, 893, 1323, 1370, 1493
BstV2I GAAGAC 2 cut(s) 621, 1367
BstX2I RGATCY 1 cut(s) 19
BstYI RGATCY 1 cut(s) 19
Bsu15I ATCGAT 1 cut(s) 489
BsuTUI ATCGAT 1 cut(s) 489
BtgI CCRYGG 1 cut(s) 1671
BtgZI GCGATG 2 cut(s) 322, 677
BtsCI GGATG 1 cut(s) 1273
BtsI GCAGTG 1 cut(s) 116
BtsIMutI CAGTG 6 cut(s) 55, 116, 195, 708, 993, 1023
BtuMI TCGCGA 1 cut(s) 662
Cac8I GCNNGC 4 cut(s) 246, 587, 1178, 1348
CaiI CAGNNNCTG 1 cut(s) 1484
CciI TCATGA 1 cut(s) 1041
CfoI GCGC 3 cut(s) 881, 1273, 1352
Cfr13I GGNCC 1 cut(s) 698
ClaI ATCGAT 1 cut(s) 489
CviAII CATG 6 cut(s) 95, 136, 301, 1009, 1042, 1355
DdeI CTNAG 6 cut(s) 31, 252, 629, 720, 1362, 1502
DpnI GATC 8 cut(s) 21, 419, 659, 969, 1002, 1098, 1371, 1794
DpnII GATC 8 cut(s) 19, 417, 657, 967, 1000, 1096, 1369, 1792
DraIII CACNNNGTG 1 cut(s) 349
Eam1104I CTCTTC 1 cut(s) 735
EarI CTCTTC 1 cut(s) 735
Eco47I GGWCC 1 cut(s) 698
Eco57I CTGAAG 3 cut(s) 252, 633, 1251
Eco88I CYCGRG 2 cut(s) 831, 1052
EcoRII CCWGG 1 cut(s) 220
FaeI CATG 6 cut(s) 98, 139, 304, 1012, 1045, 1358
FatI CATG 6 cut(s) 94, 135, 300, 1008, 1041, 1354
FblI GTMKAC 3 cut(s) 913, 1254, 1423
Fnu4HI GCNGC 8 cut(s) 249, 315, 578, 590, 882, 1312, 1359, 1482
FokI GGATG 1 cut(s) 1260
Fsp4HI GCNGC 8 cut(s) 249, 315, 578, 590, 882, 1312, 1359, 1482
FspBI CTAG 5 cut(s) 78, 245, 326, 672, 1221
GlaI GCGC 3 cut(s) 880, 1272, 1351
GluI GCNGC 8 cut(s) 249, 315, 578, 590, 882, 1312, 1359, 1482
GsuI CTGGAG 2 cut(s) 1017, 1440
HhaI GCGC 3 cut(s) 881, 1273, 1352
Hin1II CATG 6 cut(s) 98, 139, 304, 1012, 1045, 1358
Hin6I GCGC 3 cut(s) 879, 1271, 1350
HinP1I GCGC 3 cut(s) 879, 1271, 1350
HincII GTYRAC 1 cut(s) 1255
HindII GTYRAC 1 cut(s) 1255
HindIII AAGCTT 3 cut(s) 105, 375, 1529
HphI GGTGA 3 cut(s) 79, 1118, 1644
Hpy166II GTNNAC 5 cut(s) 914, 1127, 1255, 1424, 1652
Hpy8I GTNNAC 5 cut(s) 914, 1127, 1255, 1424, 1652
Hpy99I CGWCG 2 cut(s) 1256, 1520
HpyAV CCTTC 4 cut(s) 546, 833, 990, 1061
HpyCH4III ACNGT 8 cut(s) 91, 199, 424, 625, 904, 1027, 1218, 1766
HpyCH4IV ACGT 3 cut(s) 649, 1117, 1420
HpyF10VI GCNNNNNNNGC 6 cut(s) 528, 586, 668, 1154, 1358, 1687
HpyF3I CTNAG 6 cut(s) 31, 252, 629, 720, 1362, 1502
HpySE526I ACGT 3 cut(s) 649, 1117, 1420
Hsp92II CATG 6 cut(s) 98, 139, 304, 1012, 1045, 1358
HspAI GCGC 3 cut(s) 879, 1271, 1350
Kzo9I GATC 8 cut(s) 19, 417, 657, 967, 1000, 1096, 1369, 1792
LmnI GCTCC 1 cut(s) 1511
Lsp1109I GCAGC 7 cut(s) 326, 589, 601, 893, 1323, 1370, 1493
LweI GCATC 4 cut(s) 481, 495, 1282, 1758
MaeI CTAG 5 cut(s) 78, 245, 326, 672, 1221
MaeII ACGT 3 cut(s) 649, 1117, 1420
MaeIII GTNAC 4 cut(s) 85, 554, 1225, 1435
MalI GATC 8 cut(s) 21, 419, 659, 969, 1002, 1098, 1371, 1794
MboI GATC 8 cut(s) 19, 417, 657, 967, 1000, 1096, 1369, 1792
MfeI CAATTG 1 cut(s) 495
MflI RGATCY 1 cut(s) 19
MhlI GDGCHC 2 cut(s) 287, 963
MluCI AATT 7 cut(s) 495, 744, 944, 1045, 1138, 1152, 1184
MlyI GAGTC 1 cut(s) 474
MmeI TCCRAC 2 cut(s) 150, 720
MroXI GAANNNNTTC 1 cut(s) 1071
MseI TTAA 7 cut(s) 270, 620, 681, 1079, 1266, 1316, 1533
MslI CAYNNNNRTG 2 cut(s) 299, 1040
MspA1I CMGCKG 2 cut(s) 317, 1484
MspCI CTTAAG 2 cut(s) 1078, 1265
MspR9I CCNGG 1 cut(s) 222
MunI CAATTG 1 cut(s) 495
Mva1269I GAATGC 1 cut(s) 1035
MvaI CCWGG 1 cut(s) 222
MvnI CGCG 3 cut(s) 662, 879, 1539
MwoI GCNNNNNNNGC 6 cut(s) 528, 586, 668, 1154, 1358, 1687
NdeII GATC 8 cut(s) 19, 417, 657, 967, 1000, 1096, 1369, 1792
NheI GCTAGC 1 cut(s) 244
NlaIII CATG 6 cut(s) 98, 139, 304, 1012, 1045, 1358
NmuCI GTSAC 2 cut(s) 85, 1435
NruI TCGCGA 1 cut(s) 662
PaeR7I CTCGAG 2 cut(s) 831, 1052
PagI TCATGA 1 cut(s) 1041
PctI GAATGC 1 cut(s) 1035
PdmI GAANNNNTTC 1 cut(s) 1071
PfeI GAWTC 9 cut(s) 173, 185, 191, 638, 802, 976, 1445, 1451, 1642
PflFI GACNNNGTC 1 cut(s) 696
PflMI CCANNNNNTGG 1 cut(s) 54
PfoI TCCNGGA 1 cut(s) 220
PkrI GCNGC 8 cut(s) 250, 316, 579, 591, 883, 1313, 1360, 1483
PleI GAGTC 1 cut(s) 473
PpsI GAGTC 1 cut(s) 473
Psp6I CCWGG 1 cut(s) 220
PspGI CCWGG 1 cut(s) 220
PspPI GGNCC 1 cut(s) 698
PstNI CAGNNNCTG 1 cut(s) 1484
PsuI RGATCY 1 cut(s) 19
PsyI GACNNNGTC 1 cut(s) 696
PvuII CAGCTG 1 cut(s) 1484
RruI TCGCGA 1 cut(s) 662
RseI CAYNNNNRTG 2 cut(s) 299, 1040
SalI GTCGAC 1 cut(s) 1253
SaqAI TTAA 7 cut(s) 270, 620, 681, 1079, 1266, 1316, 1533
SatI GCNGC 8 cut(s) 249, 315, 578, 590, 882, 1312, 1359, 1482
Sau3AI GATC 8 cut(s) 19, 417, 657, 967, 1000, 1096, 1369, 1792
Sau96I GGNCC 1 cut(s) 698
SchI GAGTC 1 cut(s) 474
ScrFI CCNGG 1 cut(s) 222
SduI GDGCHC 2 cut(s) 287, 963
SfaNI GCATC 4 cut(s) 481, 495, 1282, 1758
Sfr274I CTCGAG 2 cut(s) 831, 1052
SinI GGWCC 1 cut(s) 698
SlaI CTCGAG 2 cut(s) 831, 1052
SmiMI CAYNNNNRTG 2 cut(s) 299, 1040
SmlI CTYRAG 6 cut(s) 66, 398, 831, 1052, 1078, 1265
SmoI CTYRAG 6 cut(s) 66, 398, 831, 1052, 1078, 1265
Sse9I AATT 7 cut(s) 495, 744, 944, 1045, 1138, 1152, 1184
SsiI CCGC 3 cut(s) 248, 317, 552
SspI AATATT 1 cut(s) 888
SspMI CTAG 5 cut(s) 78, 245, 326, 672, 1221
StyD4I CCNGG 1 cut(s) 220
TaaI ACNGT 8 cut(s) 91, 199, 424, 625, 904, 1027, 1218, 1766
TaiI ACGT 3 cut(s) 652, 1120, 1423
TasI AATT 7 cut(s) 495, 744, 944, 1045, 1138, 1152, 1184
TauI GCSGC 1 cut(s) 251
TfiI GAWTC 9 cut(s) 173, 185, 191, 638, 802, 976, 1445, 1451, 1642
Tru1I TTAA 7 cut(s) 270, 620, 681, 1079, 1266, 1316, 1533
Tru9I TTAA 7 cut(s) 270, 620, 681, 1079, 1266, 1316, 1533
TscAI CASTG 6 cut(s) 55, 123, 202, 708, 1000, 1030
TseFI GTSAC 2 cut(s) 85, 1435
TseI GCWGC 7 cut(s) 314, 577, 589, 881, 1311, 1358, 1481
Tsp45I GTSAC 2 cut(s) 85, 1435
TspRI CASTG 6 cut(s) 55, 123, 202, 708, 1000, 1030
Tth111I GACNNNGTC 1 cut(s) 696
Van91I CCANNNNNTGG 1 cut(s) 54
Vha464I CTTAAG 2 cut(s) 1078, 1265
VpaK11BI GGWCC 1 cut(s) 698
XapI RAATTY 2 cut(s) 744, 1184
XbaI TCTAGA 1 cut(s) 325
XhoI CTCGAG 2 cut(s) 831, 1052
XmiI GTMKAC 3 cut(s) 913, 1254, 1423
XmnI GAANNNNTTC 1 cut(s) 1071
XspI CTAG 5 cut(s) 78, 245, 326, 672, 1221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.