MD15G1293800.v1.1

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0007199
Physical Location & Seq
Forward (+)
27333930 .. 27336944
3015 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1293800.v1.1.491

Sequence Viewer

Length: 1791 bp
ATGTCCTCTAAGAAGAAGGAGCTTCTTTCTTCTGCCTTGAAGAGAACCAGTGAATGGATTTTTTCGCAGGAGATTCCGAGCGATATTAGTATTAATGTTGGAGGAGTTTCCTTCTCATTGCACAAGAAACTGATATCAGAATCTAGTTATGCTGCTGATCTATCTGTTATTGAGCTATCTGATGTTCCGGGTGGATCAGAAGCGTTTGAGCTTGCAGCAAGATTCTGTTATGGAATAAATTTCGAGTTAAGCACAGGAAACATCGCAATGCTGCGATGCGTGGCTGAGTATCTTCTGATGACTGAGGACTACGCGGTTGGAAACCTTGTGGGAAGAGCTGATGCATACTTGAATGAAGTGGCATTGAAAAGCCTAGCAGGTGCAGTTACTGTTTTGCACATGTCAGAAAATTTCCTCCCAATGGCGGAGAAGGTAAAATTAGTGAGCCGGTGCATAGATGCAATTGCTTTTATGGCCTGCAAAGAGGGCCAGTTCAGTGTGTCCGGTAGGACTGATGGTGGCATTGAGGGTGCGGTTTTTTCAGCTGTGTCTCATCCAAAGCCTGTTGTGGATTGGTGGGCTGAAGACTTAACTGTTCTTAGAATTGATATCTTCCAACGCGTTATGATTGCAATGTTGGCAAGAGGGTTTAAGCAGTATGCTCTTGGACCAGTGCTCATGCTATATGCACAGAAATCGCTACGAGGTTTGGAAATATTTGGAAAGGGGAGGACGAAAATCGAGCTGCGACAAGAGCACGAGAAGAGGGTTGTCTTAGAAACAATTGTGAGTCTTCTGCCAAGGGAGAAAAATGCAATGTCAGTCAGCTTTCTTTCCATGCTTCTTCGTGCTGCGAAAACCCTTGAAACAACGGTTGCTTGCCAGCTAGATTTGGAGAAGAGAATGGGGTTGCAGTTGGCACAGGCTGTTTTAGATGATCTCTTGATTCCATCATATTCCCTTACCGGGGACACATTGCTTGATGTGGAGACTGTGCAAAGGATCATGACGAATTACCTTGAATATGAAACGGAGGGGAGCCGTTTGGGATACAATGCAGAGGACGATTTTGTTTCTCCTGCACTAACAGACATGGAACGCGTTGGAAAGCTAATGGAGAACTACCTGGCTGAAATAGCCACTGACCGCAACATATCTGTCTCTAAGTTCATTGGCATTGCTGAACTTATTCCAGAACAATCAAGGGCAACAGAAGATGGCATGTATAGAGCCATTGATATCTACCTAAAGGCTCATCCTGCTCTAAGTGATATGGAGAGAAAGAAAGTGTGCAGTATGATGGACTGCCAGACGCTTTCACGAGAGGCCTGCACTCACGCTGCACAGAATGACCGCCTTCCTGTTCACACTGTGGTCCAAGTGCTATACTATGAGCAGCAGCGCCTTCGAGATGTAATGAATGGCAACATAGGTGGTGAATCCTCTGTTCTTCCTTCCAAAGGGAGCGTACACTCCACTGATATCCACACGGTTCCTGATGAGCTCACCATCTTGCGTAGACAAAATGCGGAGCTAAAAATAGAGTTGGTGAAGATGAAGATGAAGCTGAAAGAACTCGAAACGTCTACTATGAGAACAGCAATGAGCAGTCCAATGGGAAACACTCCAATATCTGCTGACAAACCTCCTCTGCCTCGAAAATCATTTATGAACTTGGTGTCGAAAAGACTTGGGAAGCTTTCTCCCTTTGTGCGTGCAGATGGAGTGACACCTACTGGTACCAAAGGCCGGACCAAACCTTCCAAAGATCGGCGGCATTCAATTTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0042742 GO:0043207 GO:0050896 GO:0051704 GO:0051707 GO:0098542
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

597

Amino Acids

66.1

Weight (kDa)

6.82

Isoelectric Point (pI)

40.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 191 - 444 3.6e-88 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 368
Acc36I ACCTGC 1 cut(s) 368
Acc65I GGTACC 1 cut(s) 1739
AccB1I GGYRCC 1 cut(s) 1739
AccB7I CCANNNNNTGG 1 cut(s) 54
AccI GTMKAC 2 cut(s) 1519, 1586
AccII CGCG 3 cut(s) 314, 621, 1101
AciI CCGC 7 cut(s) 314, 425, 533, 1147, 1354, 1529, 1774
AclWI GGATC 2 cut(s) 202, 1010
AcsI RAATTY 2 cut(s) 238, 409
AcuI CTGAAG 1 cut(s) 603
AdeI CACNNNGTG 1 cut(s) 1372
AfaI GTAC 2 cut(s) 1470, 1741
AfiI CCNNNNNNNGG 8 cut(s) 54, 421, 424, 966, 967, 1460, 1749, 1770
AflIII ACRYGT 3 cut(s) 399, 619, 1099
AgsI TTSAA 6 cut(s) 40, 352, 367, 866, 1022, 1782
AjnI CCWGG 1 cut(s) 1125
Alw21I GWGCWC 3 cut(s) 678, 759, 1506
Alw26I GTCTC 3 cut(s) 555, 983, 1165
AlwI GGATC 2 cut(s) 202, 1010
AlwNI CAGNNNCTG 1 cut(s) 389
AoxI GGCC 4 cut(s) 474, 487, 1326, 1747
ApeKI GCWGC 8 cut(s) 152, 215, 271, 745, 851, 1340, 1396, 1399
ApoI RAATTY 2 cut(s) 238, 409
AseI ATTAAT 1 cut(s) 93
Asp700I GAANNNNTTC 1 cut(s) 1188
Asp718I GGTACC 1 cut(s) 1739
AspLEI GCGC 1 cut(s) 1404
AspS9I GGNCC 4 cut(s) 487, 668, 1375, 1752
AsuC2I CCSGG 2 cut(s) 189, 967
AsuHPI GGTGA 3 cut(s) 1448, 1498, 1561
AvaII GGWCC 3 cut(s) 668, 1375, 1752
BanI GGYRCC 1 cut(s) 1739
BanII GRGCYC 1 cut(s) 1506
BauI CACGAG 2 cut(s) 758, 1320
BbsI GAAGAC 2 cut(s) 591, 785
Bbv12I GWGCWC 3 cut(s) 678, 759, 1506
BbvI GCAGC 8 cut(s) 139, 227, 258, 732, 838, 1327, 1408, 1411
BccI CCATC 6 cut(s) 509, 958, 1211, 1294, 1517, 1715
BceAI ACGGC 1 cut(s) 1026
BcgI CGANNNNNNTGC 6 cut(s) 678, 712, 1311, 1345, 1388, 1422
BciT130I CCWGG 1 cut(s) 1127
BciVI GTATCC 1 cut(s) 1043
BcnI CCSGG 2 cut(s) 189, 967
BcoDI GTCTC 3 cut(s) 555, 983, 1165
BfaI CTAG 3 cut(s) 144, 374, 887
BfoI RGCGCY 1 cut(s) 1405
BfuAI ACCTGC 1 cut(s) 368
BfuI GTATCC 1 cut(s) 1043
BisI GCNGC 9 cut(s) 153, 216, 272, 746, 852, 1341, 1397, 1400, 1775
BlsI GCNGC 9 cut(s) 154, 217, 273, 747, 853, 1342, 1398, 1401, 1776
Bme1390I CCNGG 3 cut(s) 189, 967, 1127
Bme18I GGWCC 3 cut(s) 668, 1375, 1752
BmgT120I GGNCC 4 cut(s) 487, 668, 1375, 1752
BmiI GGNNCC 3 cut(s) 1040, 1494, 1741
BmrFI CCNGG 3 cut(s) 189, 967, 1127
BmsI GCATC 3 cut(s) 266, 331, 448
BpiI GAAGAC 2 cut(s) 591, 785
BpuMI CCSGG 2 cut(s) 189, 967
BsaJI CCNNGG 2 cut(s) 800, 966
BsaWI WCCGGW 1 cut(s) 503
Bsc4I CCNNNNNNNGG 8 cut(s) 54, 421, 424, 966, 967, 1460, 1749, 1770
Bse118I RCCGGY 1 cut(s) 447
Bse1I ACTGG 4 cut(s) 48, 490, 671, 1741
Bse3DI GCAATG 7 cut(s) 116, 273, 639, 822, 974, 1176, 1608
BseBI CCWGG 1 cut(s) 1127
BseDI CCNNGG 2 cut(s) 800, 966
BseGI GGATG 2 cut(s) 553, 1255
BseLI CCNNNNNNNGG 8 cut(s) 54, 421, 424, 966, 967, 1460, 1749, 1770
BseMI GCAATG 7 cut(s) 116, 273, 639, 822, 974, 1176, 1608
BseMII CTCAG 2 cut(s) 276, 294
BseNI ACTGG 4 cut(s) 48, 490, 671, 1741
BseRI GAGGAG 2 cut(s) 117, 1638
BseXI GCAGC 8 cut(s) 139, 227, 258, 732, 838, 1327, 1408, 1411
BsgI GTGCAG 6 cut(s) 402, 1065, 1312, 1315, 1326, 1737
Bsh1236I CGCG 3 cut(s) 314, 621, 1101
BshFI GGCC 4 cut(s) 476, 489, 1328, 1749
BshNI GGYRCC 1 cut(s) 1739
BsiHKAI GWGCWC 3 cut(s) 678, 759, 1506
BsiSI CCGG 5 cut(s) 188, 448, 504, 966, 1750
BslFI GGGAC 1 cut(s) 983
BslI CCNNNNNNNGG 8 cut(s) 54, 421, 424, 966, 967, 1460, 1749, 1770
BsmAI GTCTC 3 cut(s) 555, 983, 1165
BsmFI GGGAC 1 cut(s) 983
BsmI GAATGC 1 cut(s) 1777
BsnI GGCC 4 cut(s) 476, 489, 1328, 1749
Bsp1286I GDGCHC 3 cut(s) 678, 759, 1506
Bsp143I GATC 5 cut(s) 157, 194, 937, 1002, 1768
BspACI CCGC 7 cut(s) 314, 425, 533, 1147, 1354, 1529, 1774
BspANI GGCC 4 cut(s) 476, 489, 1328, 1749
BspCNI CTCAG 2 cut(s) 277, 295
BspFNI CGCG 3 cut(s) 314, 621, 1101
BspHI TCATGA 1 cut(s) 1005
BspLI GGNNCC 3 cut(s) 1040, 1494, 1741
BspMI ACCTGC 1 cut(s) 368
BspPI GGATC 2 cut(s) 202, 1010
BspQI GCTCTTC 1 cut(s) 328
BspT107I GGYRCC 1 cut(s) 1739
BsrDI GCAATG 7 cut(s) 116, 273, 639, 822, 974, 1176, 1608
BsrFI RCCGGY 1 cut(s) 447
BsrI ACTGG 4 cut(s) 48, 490, 671, 1741
BssAI RCCGGY 1 cut(s) 447
BssECI CCNNGG 2 cut(s) 800, 966
BssMI GATC 5 cut(s) 157, 194, 937, 1002, 1768
BssSI CACGAG 2 cut(s) 758, 1320
BssT1I CCWWGG 1 cut(s) 800
Bst2BI CACGAG 2 cut(s) 758, 1320
Bst2UI CCWGG 1 cut(s) 1127
Bst4CI ACNGT 6 cut(s) 391, 595, 874, 994, 1372, 1492
Bst6I CTCTTC 4 cut(s) 35, 328, 758, 893
BstC8I GCNNGC 6 cut(s) 213, 478, 880, 884, 1330, 1716
BstDEI CTNAG 7 cut(s) 9, 285, 303, 599, 775, 1164, 1265
BstENI CCTNNNNNAGG 1 cut(s) 1458
BstF5I GGATG 2 cut(s) 553, 1255
BstFNI CGCG 3 cut(s) 314, 621, 1101
BstH2I RGCGCY 1 cut(s) 1405
BstHHI GCGC 1 cut(s) 1404
BstKTI GATC 5 cut(s) 160, 197, 940, 1005, 1771
BstMAI GTCTC 3 cut(s) 555, 983, 1165
BstMBI GATC 5 cut(s) 157, 194, 937, 1002, 1768
BstMWI GCNNNNNNNGC 6 cut(s) 473, 486, 638, 754, 1136, 1259
BstNI CCWGG 1 cut(s) 1127
BstNSI RCATGY 2 cut(s) 403, 1225
BstSCI CCNGG 3 cut(s) 187, 965, 1125
BstUI CGCG 3 cut(s) 314, 621, 1101
BstV1I GCAGC 8 cut(s) 139, 227, 258, 732, 838, 1327, 1408, 1411
BstV2I GAAGAC 2 cut(s) 591, 785
BsuI GTATCC 1 cut(s) 1043
BsuRI GGCC 4 cut(s) 476, 489, 1328, 1749
BtgZI GCGATG 2 cut(s) 247, 289
BtsCI GGATG 2 cut(s) 553, 1255
BtsIMutI CAGTG 6 cut(s) 55, 502, 678, 1140, 1368, 1476
BveI ACCTGC 1 cut(s) 368
Cac8I GCNNGC 6 cut(s) 213, 478, 880, 884, 1330, 1716
CaiI CAGNNNCTG 1 cut(s) 389
CciI TCATGA 1 cut(s) 1005
CfoI GCGC 1 cut(s) 1404
Cfr10I RCCGGY 1 cut(s) 447
Cfr13I GGNCC 4 cut(s) 487, 668, 1375, 1752
CseI GACGC 1 cut(s) 1321
Csp6I GTAC 2 cut(s) 1469, 1740
CspCI CAANNNNNGTGG 2 cut(s) 1414, 1449
CviAII CATG 6 cut(s) 400, 679, 838, 1006, 1093, 1222
CviQI GTAC 2 cut(s) 1469, 1740
DdeI CTNAG 7 cut(s) 9, 285, 303, 599, 775, 1164, 1265
DpnI GATC 5 cut(s) 159, 196, 939, 1004, 1770
DpnII GATC 5 cut(s) 157, 194, 937, 1002, 1768
DraIII CACNNNGTG 1 cut(s) 1372
Eam1104I CTCTTC 4 cut(s) 35, 328, 758, 893
EarI CTCTTC 4 cut(s) 35, 328, 758, 893
EciI GGCGGA 1 cut(s) 440
Ecl136II GAGCTC 1 cut(s) 1504
Eco130I CCWWGG 1 cut(s) 800
Eco147I AGGCCT 1 cut(s) 1328
Eco24I GRGCYC 1 cut(s) 1506
Eco32I GATATC 4 cut(s) 135, 610, 1240, 1483
Eco47I GGWCC 3 cut(s) 668, 1375, 1752
Eco53kI GAGCTC 1 cut(s) 1504
Eco57I CTGAAG 1 cut(s) 603
EcoICRI GAGCTC 1 cut(s) 1504
EcoNI CCTNNNNNAGG 1 cut(s) 1458
EcoRII CCWGG 1 cut(s) 1125
EcoRV GATATC 4 cut(s) 135, 610, 1240, 1483
EcoT14I CCWWGG 1 cut(s) 800
EcoT22I ATGCAT 1 cut(s) 346
EcoT38I GRGCYC 1 cut(s) 1506
ErhI CCWWGG 1 cut(s) 800
FaeI CATG 6 cut(s) 403, 682, 841, 1009, 1096, 1225
FaqI GGGAC 1 cut(s) 983
FatI CATG 6 cut(s) 399, 678, 837, 1005, 1092, 1221
FblI GTMKAC 2 cut(s) 1519, 1586
Fnu4HI GCNGC 9 cut(s) 153, 216, 272, 746, 852, 1341, 1397, 1400, 1775
FokI GGATG 2 cut(s) 540, 1242
FriOI GRGCYC 1 cut(s) 1506
Fsp4HI GCNGC 9 cut(s) 153, 216, 272, 746, 852, 1341, 1397, 1400, 1775
FspBI CTAG 3 cut(s) 144, 374, 887
GlaI GCGC 1 cut(s) 1403
GluI GCNGC 9 cut(s) 153, 216, 272, 746, 852, 1341, 1397, 1400, 1775
HaeII RGCGCY 1 cut(s) 1405
HaeIII GGCC 4 cut(s) 476, 489, 1328, 1749
HapII CCGG 5 cut(s) 188, 448, 504, 966, 1750
HgaI GACGC 1 cut(s) 1321
HhaI GCGC 1 cut(s) 1404
Hin1II CATG 6 cut(s) 403, 682, 841, 1009, 1096, 1225
Hin6I GCGC 1 cut(s) 1402
HinP1I GCGC 1 cut(s) 1402
HindIII AAGCTT 1 cut(s) 1697
HinfI GANTC 6 cut(s) 73, 140, 222, 790, 946, 1439
HpaII CCGG 5 cut(s) 188, 448, 504, 966, 1750
HphI GGTGA 3 cut(s) 1448, 1498, 1561
Hpy166II GTNNAC 4 cut(s) 1366, 1471, 1520, 1587
Hpy188I TCNGA 6 cut(s) 78, 139, 181, 199, 297, 406
Hpy188III TCNNGA 7 cut(s) 943, 1006, 1193, 1320, 1409, 1496, 1788
Hpy8I GTNNAC 4 cut(s) 1366, 1471, 1520, 1587
HpyAV CCTTC 7 cut(s) 10, 121, 424, 1367, 1415, 1464, 1770
HpyCH4III ACNGT 6 cut(s) 391, 595, 874, 994, 1372, 1492
HpyCH4IV ACGT 1 cut(s) 1583
HpyF10VI GCNNNNNNNGC 6 cut(s) 473, 486, 638, 754, 1136, 1259
HpyF3I CTNAG 7 cut(s) 9, 285, 303, 599, 775, 1164, 1265
HpySE526I ACGT 1 cut(s) 1583
Hsp92II CATG 6 cut(s) 403, 682, 841, 1009, 1096, 1225
HspAI GCGC 1 cut(s) 1402
KpnI GGTACC 1 cut(s) 1743
Kzo9I GATC 5 cut(s) 157, 194, 937, 1002, 1768
LguI GCTCTTC 1 cut(s) 328
LmnI GCTCC 4 cut(s) 19, 1038, 1464, 1531
Lsp1109I GCAGC 8 cut(s) 139, 227, 258, 732, 838, 1327, 1408, 1411
LweI GCATC 3 cut(s) 266, 331, 448
MaeI CTAG 3 cut(s) 144, 374, 887
MaeII ACGT 1 cut(s) 1583
MaeIII GTNAC 2 cut(s) 385, 1726
MalI GATC 5 cut(s) 159, 196, 939, 1004, 1770
MboI GATC 5 cut(s) 157, 194, 937, 1002, 1768
MfeI CAATTG 2 cut(s) 462, 783
MhlI GDGCHC 3 cut(s) 678, 759, 1506
MluCI AATT 8 cut(s) 238, 409, 437, 462, 603, 783, 1012, 1782
MluI ACGCGT 2 cut(s) 619, 1099
MlyI GAGTC 1 cut(s) 799
MmeI TCCRAC 4 cut(s) 79, 298, 640, 1084
Mph1103I ATGCAT 1 cut(s) 346
MroXI GAANNNNTTC 1 cut(s) 1188
MseI TTAA 4 cut(s) 93, 248, 590, 651
MslI CAYNNNNRTG 1 cut(s) 266
MspA1I CMGCKG 1 cut(s) 545
MspI CCGG 5 cut(s) 188, 448, 504, 966, 1750
MspR9I CCNGG 3 cut(s) 189, 967, 1127
MunI CAATTG 2 cut(s) 462, 783
Mva1269I GAATGC 1 cut(s) 1777
MvaI CCWGG 1 cut(s) 1127
MvnI CGCG 3 cut(s) 314, 621, 1101
MwoI GCNNNNNNNGC 6 cut(s) 473, 486, 638, 754, 1136, 1259
NciI CCSGG 2 cut(s) 189, 967
NdeII GATC 5 cut(s) 157, 194, 937, 1002, 1768
NlaIII CATG 6 cut(s) 403, 682, 841, 1009, 1096, 1225
NlaIV GGNNCC 3 cut(s) 1040, 1494, 1741
NmuCI GTSAC 1 cut(s) 1726
NsiI ATGCAT 1 cut(s) 346
NspI RCATGY 2 cut(s) 403, 1225
PagI TCATGA 1 cut(s) 1005
PaqCI CACCTGC 1 cut(s) 368
PceI AGGCCT 1 cut(s) 1328
PciI ACATGT 1 cut(s) 399
PciSI GCTCTTC 1 cut(s) 328
PctI GAATGC 1 cut(s) 1777
PdmI GAANNNNTTC 1 cut(s) 1188
PfeI GAWTC 5 cut(s) 73, 140, 222, 946, 1439
PflMI CCANNNNNTGG 1 cut(s) 54
PkrI GCNGC 9 cut(s) 154, 217, 273, 747, 853, 1342, 1398, 1401, 1776
PleI GAGTC 1 cut(s) 798
PpsI GAGTC 1 cut(s) 798
PscI ACATGT 1 cut(s) 399
PshBI ATTAAT 1 cut(s) 93
Psp124BI GAGCTC 1 cut(s) 1506
Psp6I CCWGG 1 cut(s) 1125
PspGI CCWGG 1 cut(s) 1125
PspN4I GGNNCC 3 cut(s) 1040, 1494, 1741
PspPI GGNCC 4 cut(s) 487, 668, 1375, 1752
PstNI CAGNNNCTG 1 cut(s) 389
PvuII CAGCTG 1 cut(s) 545
RsaI GTAC 2 cut(s) 1470, 1741
RsaNI GTAC 2 cut(s) 1469, 1740
RseI CAYNNNNRTG 1 cut(s) 266
SacI GAGCTC 1 cut(s) 1506
SapI GCTCTTC 1 cut(s) 328
SaqAI TTAA 4 cut(s) 93, 248, 590, 651
SatI GCNGC 9 cut(s) 153, 216, 272, 746, 852, 1341, 1397, 1400, 1775
Sau3AI GATC 5 cut(s) 157, 194, 937, 1002, 1768
Sau96I GGNCC 4 cut(s) 487, 668, 1375, 1752
SchI GAGTC 1 cut(s) 799
ScrFI CCNGG 3 cut(s) 189, 967, 1127
SduI GDGCHC 3 cut(s) 678, 759, 1506
SfaNI GCATC 3 cut(s) 266, 331, 448
SinI GGWCC 3 cut(s) 668, 1375, 1752
SmiMI CAYNNNNRTG 1 cut(s) 266
Sse9I AATT 8 cut(s) 238, 409, 437, 462, 603, 783, 1012, 1782
SseBI AGGCCT 1 cut(s) 1328
SsiI CCGC 7 cut(s) 314, 425, 533, 1147, 1354, 1529, 1774
SspI AATATT 1 cut(s) 717
SspMI CTAG 3 cut(s) 144, 374, 887
SstI GAGCTC 1 cut(s) 1506
StuI AGGCCT 1 cut(s) 1328
StyD4I CCNGG 3 cut(s) 187, 965, 1125
StyI CCWWGG 1 cut(s) 800
TaaI ACNGT 6 cut(s) 391, 595, 874, 994, 1372, 1492
TaiI ACGT 1 cut(s) 1586
TaqI TCGA 6 cut(s) 243, 741, 1408, 1578, 1657, 1682
TasI AATT 8 cut(s) 238, 409, 437, 462, 603, 783, 1012, 1782
TauI GCSGC 1 cut(s) 1777
TfiI GAWTC 5 cut(s) 73, 140, 222, 946, 1439
Tru1I TTAA 4 cut(s) 93, 248, 590, 651
Tru9I TTAA 4 cut(s) 93, 248, 590, 651
TscAI CASTG 6 cut(s) 55, 502, 678, 1147, 1375, 1483
TseFI GTSAC 1 cut(s) 1726
TseI GCWGC 8 cut(s) 152, 215, 271, 745, 851, 1340, 1396, 1399
Tsp45I GTSAC 1 cut(s) 1726
TspDTI ATGAA 7 cut(s) 369, 1041, 1159, 1433, 1571, 1577, 1685
TspGWI ACGGA 1 cut(s) 1046
TspRI CASTG 6 cut(s) 55, 502, 678, 1147, 1375, 1483
Van91I CCANNNNNTGG 1 cut(s) 54
VpaK11BI GGWCC 3 cut(s) 668, 1375, 1752
VspI ATTAAT 1 cut(s) 93
XagI CCTNNNNNAGG 1 cut(s) 1458
XapI RAATTY 2 cut(s) 238, 409
XceI RCATGY 2 cut(s) 403, 1225
XmiI GTMKAC 2 cut(s) 1519, 1586
XmnI GAANNNNTTC 1 cut(s) 1188
XspI CTAG 3 cut(s) 144, 374, 887
Zsp2I ATGCAT 1 cut(s) 346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.