MD02G1182500.v1.1

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0007199
Physical Location & Seq
Forward (+)
16466479 .. 16469876
3398 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1182500.v1.1.491

Sequence Viewer

Length: 1881 bp
ATGGCGGATATCGAACTTGGCGAGGAGAGTACTGCACCCACTACCACAGCCAACATGTCCTTTAAGAAGAAGGAGCTTCTTTCTTCTGCCTTGAAGAGAACTAGTGAATGGATTTTTTCGCAGGAGATTCCGAGCGATATTAGTATTAATGTTGGAGGGGTTTCCTTCTCATTGCACAAGTTCCCTTTGATTTCGAAGTGTGGACACATAAGGAAACTGATATCAGAATCGAGTGATGCTGCTGATCTATCTGTCATTGAACTATCTGATGTTCCGGGCGGATCAGAAGCATTTGAGCTTGCAGCAAAATTCTGCTATGGAATAAATTTCGAGATAAGCACAGAGAACATCGCAATGCTCCGATGTGTGGCAGAGTATCTTCTGATGACTGAGGACTACGGGGTTGGAAACCTTGTGGGAAGAGCTGATGCATACTTAAATGAAGTGGCATTGAAGAGCCTAGCGGGTGCAGTTACTGTTTTGCACATGTCAGAAAGTTTCCTCCCAATGGCGGAGAAGGTAAAATTAGTGAACCGCTGCATAGATGCAATTGCTTTTATAGCCTGCAAAGAGAGCCAGTTCAGTGTGTCCAGTAGGGCTGATGGCGGCAATGAGGGTGCAGTTTCTTCAGCTGTGTATCATCCAAAGCCTGTTGTGGATTGGTGGGCTGAAGACTTAATTGTTCTTAGAATCGATATGTTCCAACGTGTTATGATTGCAATGATGGCAAGAGGGTTTAAGCAATATGCTCTCGGACCAGTACTCATGCTCTATGCGCAGAAATCGCTAAGAGGTTTGGAAATATTTGGAAAGGGGAGGAAGAAAATCGAGCCGCGACAAGAGCACGAGAAGAGGGTTGTCTTAGAAACAATTGTGAGTCTTCTGCCAAGGGCGAAAAATGCAATGTCGGTTAGCTTTCTTTCCATGCTTCTTCGTGCTGCGAAAACCCTGGAGACAACAGTTGCTTGCCAGCTAGATTTGGAGAAGAGAATGGGGTTTCAATTGGCACAGGCTGTTTTAGATGATCTCTTGATTCCATCATATTCCTATACCGGGGACACATTGCTTGATGTCGACACTGTGCAAAGGATCGTGACGAATTACCTTGAATATGAAATGCAGGGGAGCCGTTTGGGACATAATGCAGAGGATGATTTTGGTTCTCCTCCGCCGACTGACATGGAACGTGTCGGAAAGCTAATGGAGAACTACCTGGCTGAAATAGCCACTGACCGTAACGTATCTGTCTCAAAGTTCATAGGCATTGCTGAACTAATTCCAGAACAATCAAGGGAAACAGAAGATGGCATGTATAGAGCAATAGATATCTACCTAAAGGCTCATCCTGCTCTGAGTGATATGGAGAGAAAGAAAGTGTGCAGTATGATGGACTGCCAGAAACTTTCGCGCGAGGCCTGCGCTCACGCTGCGCAGAATGACCGGCTTCCTGTTCAAACTGTTGTACAAGTGCTATACTATGAGCAGCAGCGTCTTCGAGACGTCATGAATGGCAATTCCATTGGTGCTGAATCCTCTGTTCTTCCATCCAAAGGGAGCTTATACTCCACTGATATCCACCCAGTTCCTGATGAGCTCACCATCTTGCGCAGACAAAACGAGGAGCTGAAAATGGAATTGGTGAAGATGAAAATGAAACTGAAAGAACTCGAAACGTCTACTACGAGAACAGCAATGATCAGTCCAATGGGAAACACTCCAACATCTGCTGACAAACCTCCGCTGCCTCGAAAATCATTCATAAACTCAGTATCGAAAAAACTTGGGAAGCTTTCTCCCTTTGTGCGTGCAGATGGAGTGACACCTACCGGTGCCAAAGGCCGAACAAAACCTGCCAAAGATCGGCGGCATTCAATTTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0042742 GO:0043207 GO:0050896 GO:0051704 GO:0051707 GO:0098542
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

627

Amino Acids

69.38

Weight (kDa)

6.43

Isoelectric Point (pI)

37.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 220 - 473 2e-90 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1503
Acc16I TGCGCA 3 cut(s) 777, 1431, 1607
Acc36I ACCTGC 1 cut(s) 1858
AccB1I GGYRCC 1 cut(s) 1829
AccI GTMKAC 2 cut(s) 1074, 1676
AccII CGCG 3 cut(s) 835, 1408, 1410
AclWI GGATC 2 cut(s) 289, 1097
AcsI RAATTY 2 cut(s) 308, 325
AcuI CTGAAG 2 cut(s) 612, 690
AcyI GRCGYC 1 cut(s) 1500
AfaI GTAC 3 cut(s) 31, 762, 1464
AfiI CCNNNNNNNGG 6 cut(s) 367, 508, 511, 1053, 1550, 1860
AflIII ACRYGT 4 cut(s) 54, 486, 706, 1186
AgeI ACCGGT 1 cut(s) 1826
AgsI TTSAA 7 cut(s) 94, 260, 454, 1001, 1109, 1454, 1872
AhlI ACTAGT 1 cut(s) 101
AjnI CCWGG 2 cut(s) 948, 1212
AjuI GAANNNNNNNTTGG 2 cut(s) 1619, 1651
Alw21I GWGCWC 2 cut(s) 846, 1596
Alw26I GTCTC 3 cut(s) 947, 1252, 1491
AlwI GGATC 2 cut(s) 289, 1097
AlwNI CAGNNNCTG 2 cut(s) 476, 1586
AoxI GGCC 2 cut(s) 1413, 1837
ApeKI GCWGC 8 cut(s) 239, 302, 537, 938, 1427, 1483, 1486, 1741
ApoI RAATTY 2 cut(s) 308, 325
AseI ATTAAT 1 cut(s) 147
AsiGI ACCGGT 1 cut(s) 1826
Asp700I GAANNNNTTC 1 cut(s) 1275
AspLEI GCGC 5 cut(s) 778, 1410, 1421, 1432, 1608
AspS9I GGNCC 1 cut(s) 755
AsuC2I CCSGG 2 cut(s) 276, 1054
AsuHPI GGTGA 2 cut(s) 1588, 1651
AsuII TTCGAA 1 cut(s) 194
AvaII GGWCC 1 cut(s) 755
BanI GGYRCC 1 cut(s) 1829
BanII GRGCYC 1 cut(s) 1596
BauI CACGAG 1 cut(s) 845
BbsI GAAGAC 3 cut(s) 678, 872, 1484
Bbv12I GWGCWC 2 cut(s) 846, 1596
BbvI GCAGC 8 cut(s) 226, 314, 524, 925, 1414, 1495, 1498, 1728
BccI CCATC 8 cut(s) 596, 718, 1045, 1298, 1381, 1552, 1607, 1805
BceAI ACGGC 1 cut(s) 1113
BcgI CGANNNNNNTGC 2 cut(s) 1475, 1509
BciT130I CCWGG 2 cut(s) 950, 1214
BclI TGATCA 1 cut(s) 1695
BcnI CCSGG 2 cut(s) 276, 1054
BcoDI GTCTC 3 cut(s) 947, 1252, 1491
BcuI ACTAGT 1 cut(s) 101
BfaI CTAG 3 cut(s) 102, 461, 974
BfuAI ACCTGC 1 cut(s) 1858
BmcAI AGTACT 2 cut(s) 31, 762
Bme1390I CCNGG 4 cut(s) 276, 950, 1054, 1214
Bme18I GGWCC 1 cut(s) 755
BmgT120I GGNCC 1 cut(s) 755
BmiI GGNNCC 2 cut(s) 1127, 1831
BmrFI CCNGG 4 cut(s) 276, 950, 1054, 1214
BmrI ACTGGG 1 cut(s) 1574
BmsI GCATC 3 cut(s) 226, 418, 535
BmuI ACTGGG 1 cut(s) 1574
BpiI GAAGAC 3 cut(s) 678, 872, 1484
BpmI CTGGAG 1 cut(s) 971
Bpu14I TTCGAA 1 cut(s) 194
BpuMI CCSGG 2 cut(s) 276, 1054
Bsa29I ATCGAT 1 cut(s) 693
BsaHI GRCGYC 1 cut(s) 1500
BsaJI CCNNGG 3 cut(s) 887, 948, 1053
BsaWI WCCGGW 1 cut(s) 1826
Bsc4I CCNNNNNNNGG 6 cut(s) 367, 508, 511, 1053, 1550, 1860
Bse118I RCCGGY 2 cut(s) 1440, 1826
Bse1I ACTGG 4 cut(s) 577, 591, 758, 1580
Bse3DI GCAATG 8 cut(s) 170, 360, 616, 726, 909, 1061, 1263, 1698
BseBI CCWGG 2 cut(s) 950, 1214
BseCI ATCGAT 1 cut(s) 693
BseDI CCNNGG 3 cut(s) 887, 948, 1053
BseGI GGATG 4 cut(s) 640, 1156, 1342, 1544
BseLI CCNNNNNNNGG 6 cut(s) 367, 508, 511, 1053, 1550, 1860
BseMI GCAATG 8 cut(s) 170, 360, 616, 726, 909, 1061, 1263, 1698
BseMII CTCAG 3 cut(s) 381, 1343, 1779
BseNI ACTGG 4 cut(s) 577, 591, 758, 1580
BseRI GAGGAG 3 cut(s) 38, 1155, 1634
BseXI GCAGC 8 cut(s) 226, 314, 524, 925, 1414, 1495, 1498, 1728
BsgI GTGCAG 5 cut(s) 18, 489, 639, 1399, 1827
Bsh1236I CGCG 3 cut(s) 835, 1408, 1410
BshFI GGCC 2 cut(s) 1415, 1839
BshNI GGYRCC 1 cut(s) 1829
BshTI ACCGGT 1 cut(s) 1826
BshVI ATCGAT 1 cut(s) 693
BsiHKAI GWGCWC 2 cut(s) 846, 1596
BsiSI CCGG 4 cut(s) 275, 1053, 1441, 1827
BslFI GGGAC 2 cut(s) 1070, 1149
BslI CCNNNNNNNGG 6 cut(s) 367, 508, 511, 1053, 1550, 1860
BsmAI GTCTC 3 cut(s) 947, 1252, 1491
BsmBI CGTCTC 1 cut(s) 1491
BsmFI GGGAC 2 cut(s) 1070, 1149
BsmI GAATGC 1 cut(s) 1867
BsnI GGCC 2 cut(s) 1415, 1839
Bsp119I TTCGAA 1 cut(s) 194
Bsp1286I GDGCHC 2 cut(s) 846, 1596
Bsp1407I TGTACA 1 cut(s) 1462
Bsp143I GATC 6 cut(s) 244, 281, 1024, 1089, 1695, 1858
BspANI GGCC 2 cut(s) 1415, 1839
BspCNI CTCAG 3 cut(s) 382, 1344, 1778
BspDI ATCGAT 1 cut(s) 693
BspFNI CGCG 3 cut(s) 835, 1408, 1410
BspHI TCATGA 1 cut(s) 1503
BspLI GGNNCC 2 cut(s) 1127, 1831
BspMI ACCTGC 1 cut(s) 1858
BspPI GGATC 2 cut(s) 289, 1097
BspQI GCTCTTC 2 cut(s) 415, 449
BspT104I TTCGAA 1 cut(s) 194
BspT107I GGYRCC 1 cut(s) 1829
BsrDI GCAATG 8 cut(s) 170, 360, 616, 726, 909, 1061, 1263, 1698
BsrFI RCCGGY 2 cut(s) 1440, 1826
BsrGI TGTACA 1 cut(s) 1462
BsrI ACTGG 4 cut(s) 577, 591, 758, 1580
BssAI RCCGGY 2 cut(s) 1440, 1826
BssECI CCNNGG 3 cut(s) 887, 948, 1053
BssMI GATC 6 cut(s) 244, 281, 1024, 1089, 1695, 1858
BssNI GRCGYC 1 cut(s) 1500
BssSI CACGAG 1 cut(s) 845
BssT1I CCWWGG 1 cut(s) 887
Bst2BI CACGAG 1 cut(s) 845
Bst2UI CCWGG 2 cut(s) 950, 1214
Bst4CI ACNGT 5 cut(s) 478, 961, 1081, 1235, 1459
Bst6I CTCTTC 5 cut(s) 89, 415, 449, 845, 980
BstACI GRCGYC 1 cut(s) 1500
BstAUI TGTACA 1 cut(s) 1462
BstBI TTCGAA 1 cut(s) 194
BstC8I GCNNGC 6 cut(s) 300, 565, 967, 971, 1417, 1806
BstDEI CTNAG 6 cut(s) 390, 686, 788, 862, 1352, 1765
BstF5I GGATG 4 cut(s) 640, 1156, 1342, 1544
BstFNI CGCG 3 cut(s) 835, 1408, 1410
BstHHI GCGC 5 cut(s) 778, 1410, 1421, 1432, 1608
BstKTI GATC 6 cut(s) 247, 284, 1027, 1092, 1698, 1861
BstMAI GTCTC 3 cut(s) 947, 1252, 1491
BstMBI GATC 6 cut(s) 244, 281, 1024, 1089, 1695, 1858
BstNI CCWGG 2 cut(s) 950, 1214
BstNSI RCATGY 3 cut(s) 58, 490, 1312
BstSCI CCNGG 4 cut(s) 274, 948, 1052, 1212
BstUI CGCG 3 cut(s) 835, 1408, 1410
BstV1I GCAGC 8 cut(s) 226, 314, 524, 925, 1414, 1495, 1498, 1728
BstV2I GAAGAC 3 cut(s) 678, 872, 1484
Bsu15I ATCGAT 1 cut(s) 693
BsuRI GGCC 2 cut(s) 1415, 1839
BsuTUI ATCGAT 1 cut(s) 693
BtgZI GCGATG 1 cut(s) 334
BtsCI GGATG 4 cut(s) 640, 1156, 1342, 1544
BtsIMutI CAGTG 4 cut(s) 589, 1077, 1227, 1566
BveI ACCTGC 1 cut(s) 1858
Cac8I GCNNGC 6 cut(s) 300, 565, 967, 971, 1417, 1806
CaiI CAGNNNCTG 2 cut(s) 476, 1586
CciI TCATGA 1 cut(s) 1503
CfoI GCGC 5 cut(s) 778, 1410, 1421, 1432, 1608
Cfr10I RCCGGY 2 cut(s) 1440, 1826
Cfr13I GGNCC 1 cut(s) 755
ClaI ATCGAT 1 cut(s) 693
CseI GACGC 1 cut(s) 1478
Csp6I GTAC 3 cut(s) 30, 761, 1463
CspAI ACCGGT 1 cut(s) 1826
CviAII CATG 7 cut(s) 55, 487, 766, 925, 1180, 1309, 1504
CviQI GTAC 3 cut(s) 30, 761, 1463
DdeI CTNAG 6 cut(s) 390, 686, 788, 862, 1352, 1765
DpnI GATC 6 cut(s) 246, 283, 1026, 1091, 1697, 1860
DpnII GATC 6 cut(s) 244, 281, 1024, 1089, 1695, 1858
Eam1104I CTCTTC 5 cut(s) 89, 415, 449, 845, 980
EarI CTCTTC 5 cut(s) 89, 415, 449, 845, 980
EciI GGCGGA 4 cut(s) 20, 294, 527, 1158
Ecl136II GAGCTC 1 cut(s) 1594
Eco130I CCWWGG 1 cut(s) 887
Eco147I AGGCCT 1 cut(s) 1415
Eco24I GRGCYC 1 cut(s) 1596
Eco32I GATATC 4 cut(s) 10, 222, 1327, 1573
Eco47I GGWCC 1 cut(s) 755
Eco53kI GAGCTC 1 cut(s) 1594
Eco57I CTGAAG 2 cut(s) 612, 690
EcoICRI GAGCTC 1 cut(s) 1594
EcoRII CCWGG 2 cut(s) 948, 1212
EcoRV GATATC 4 cut(s) 10, 222, 1327, 1573
EcoT14I CCWWGG 1 cut(s) 887
EcoT22I ATGCAT 1 cut(s) 433
EcoT38I GRGCYC 1 cut(s) 1596
ErhI CCWWGG 1 cut(s) 887
Esp3I CGTCTC 1 cut(s) 1491
FaeI CATG 7 cut(s) 58, 490, 769, 928, 1183, 1312, 1507
FaqI GGGAC 2 cut(s) 1070, 1149
FatI CATG 7 cut(s) 54, 486, 765, 924, 1179, 1308, 1503
FauI CCCGC 1 cut(s) 457
FbaI TGATCA 1 cut(s) 1695
FblI GTMKAC 2 cut(s) 1074, 1676
FokI GGATG 4 cut(s) 627, 1163, 1329, 1531
FriOI GRGCYC 1 cut(s) 1596
FspBI CTAG 3 cut(s) 102, 461, 974
FspI TGCGCA 3 cut(s) 777, 1431, 1607
GlaI GCGC 5 cut(s) 777, 1409, 1420, 1431, 1607
GsuI CTGGAG 1 cut(s) 971
HaeIII GGCC 2 cut(s) 1415, 1839
HapII CCGG 4 cut(s) 275, 1053, 1441, 1827
HgaI GACGC 1 cut(s) 1478
HhaI GCGC 5 cut(s) 778, 1410, 1421, 1432, 1608
Hin1I GRCGYC 1 cut(s) 1500
Hin1II CATG 7 cut(s) 58, 490, 769, 928, 1183, 1312, 1507
Hin6I GCGC 5 cut(s) 776, 1408, 1419, 1430, 1606
HinP1I GCGC 5 cut(s) 776, 1408, 1419, 1430, 1606
HincII GTYRAC 1 cut(s) 1075
HindII GTYRAC 1 cut(s) 1075
HindIII AAGCTT 1 cut(s) 1787
HinfI GANTC 6 cut(s) 127, 227, 690, 877, 1033, 1529
HpaII CCGG 4 cut(s) 275, 1053, 1441, 1827
HphI GGTGA 2 cut(s) 1588, 1651
Hpy166II GTNNAC 4 cut(s) 203, 532, 1075, 1677
Hpy188III TCNNGA 8 cut(s) 331, 1030, 1093, 1280, 1496, 1504, 1586, 1878
Hpy8I GTNNAC 4 cut(s) 203, 532, 1075, 1677
HpyAV CCTTC 3 cut(s) 64, 175, 511
HpyCH4III ACNGT 5 cut(s) 478, 961, 1081, 1235, 1459
HpyCH4IV ACGT 5 cut(s) 706, 1186, 1239, 1500, 1673
HpyF3I CTNAG 6 cut(s) 390, 686, 788, 862, 1352, 1765
HpySE526I ACGT 5 cut(s) 706, 1186, 1239, 1500, 1673
Hsp92I GRCGYC 1 cut(s) 1500
Hsp92II CATG 7 cut(s) 58, 490, 769, 928, 1183, 1312, 1507
HspAI GCGC 5 cut(s) 776, 1408, 1419, 1430, 1606
Ksp22I TGATCA 1 cut(s) 1695
Kzo9I GATC 6 cut(s) 244, 281, 1024, 1089, 1695, 1858
LguI GCTCTTC 2 cut(s) 415, 449
LmnI GCTCC 5 cut(s) 73, 363, 1125, 1554, 1621
Lsp1109I GCAGC 8 cut(s) 226, 314, 524, 925, 1414, 1495, 1498, 1728
LweI GCATC 3 cut(s) 226, 418, 535
MaeI CTAG 3 cut(s) 102, 461, 974
MaeII ACGT 5 cut(s) 706, 1186, 1239, 1500, 1673
MaeIII GTNAC 4 cut(s) 472, 1093, 1235, 1816
MalI GATC 6 cut(s) 246, 283, 1026, 1091, 1697, 1860
MboI GATC 6 cut(s) 244, 281, 1024, 1089, 1695, 1858
MfeI CAATTG 3 cut(s) 549, 870, 1001
MhlI GDGCHC 2 cut(s) 846, 1596
MlyI GAGTC 1 cut(s) 886
MmeI TCCRAC 5 cut(s) 133, 385, 727, 1171, 1742
Mph1103I ATGCAT 1 cut(s) 433
MroXI GAANNNNTTC 1 cut(s) 1275
MseI TTAA 5 cut(s) 63, 147, 437, 677, 738
MslI CAYNNNNRTG 1 cut(s) 353
MspA1I CMGCKG 3 cut(s) 537, 632, 1741
MspI CCGG 4 cut(s) 275, 1053, 1441, 1827
MspR9I CCNGG 4 cut(s) 276, 950, 1054, 1214
MunI CAATTG 3 cut(s) 549, 870, 1001
Mva1269I GAATGC 1 cut(s) 1867
MvaI CCWGG 2 cut(s) 950, 1214
MvnI CGCG 3 cut(s) 835, 1408, 1410
NciI CCSGG 2 cut(s) 276, 1054
NdeII GATC 6 cut(s) 244, 281, 1024, 1089, 1695, 1858
NlaIII CATG 7 cut(s) 58, 490, 769, 928, 1183, 1312, 1507
NlaIV GGNNCC 2 cut(s) 1127, 1831
NmuCI GTSAC 2 cut(s) 1093, 1816
NsbI TGCGCA 3 cut(s) 777, 1431, 1607
NsiI ATGCAT 1 cut(s) 433
NspI RCATGY 3 cut(s) 58, 490, 1312
NspV TTCGAA 1 cut(s) 194
PagI TCATGA 1 cut(s) 1503
PceI AGGCCT 1 cut(s) 1415
PciI ACATGT 2 cut(s) 54, 486
PciSI GCTCTTC 2 cut(s) 415, 449
PcsI WCGNNNNNNNCGW 2 cut(s) 18, 1679
PctI GAATGC 1 cut(s) 1867
PdmI GAANNNNTTC 1 cut(s) 1275
PfeI GAWTC 5 cut(s) 127, 227, 690, 1033, 1529
PinAI ACCGGT 1 cut(s) 1826
PleI GAGTC 1 cut(s) 885
PpsI GAGTC 1 cut(s) 885
PscI ACATGT 2 cut(s) 54, 486
PshBI ATTAAT 1 cut(s) 147
Psp124BI GAGCTC 1 cut(s) 1596
Psp6I CCWGG 2 cut(s) 948, 1212
PspGI CCWGG 2 cut(s) 948, 1212
PspN4I GGNNCC 2 cut(s) 1127, 1831
PspPI GGNCC 1 cut(s) 755
PstNI CAGNNNCTG 2 cut(s) 476, 1586
PvuII CAGCTG 1 cut(s) 632
RsaI GTAC 3 cut(s) 31, 762, 1464
RsaNI GTAC 3 cut(s) 30, 761, 1463
RseI CAYNNNNRTG 1 cut(s) 353
SacI GAGCTC 1 cut(s) 1596
SalI GTCGAC 1 cut(s) 1073
SapI GCTCTTC 2 cut(s) 415, 449
SaqAI TTAA 5 cut(s) 63, 147, 437, 677, 738
Sau3AI GATC 6 cut(s) 244, 281, 1024, 1089, 1695, 1858
Sau96I GGNCC 1 cut(s) 755
ScaI AGTACT 2 cut(s) 31, 762
SchI GAGTC 1 cut(s) 886
ScrFI CCNGG 4 cut(s) 276, 950, 1054, 1214
SduI GDGCHC 2 cut(s) 846, 1596
SfaNI GCATC 3 cut(s) 226, 418, 535
SfuI TTCGAA 1 cut(s) 194
SinI GGWCC 1 cut(s) 755
SmiMI CAYNNNNRTG 1 cut(s) 353
SpeI ACTAGT 1 cut(s) 101
SseBI AGGCCT 1 cut(s) 1415
SspI AATATT 1 cut(s) 804
SspMI CTAG 3 cut(s) 102, 461, 974
SstI GAGCTC 1 cut(s) 1596
StuI AGGCCT 1 cut(s) 1415
StyD4I CCNGG 4 cut(s) 274, 948, 1052, 1212
StyI CCWWGG 1 cut(s) 887
TaaI ACNGT 5 cut(s) 478, 961, 1081, 1235, 1459
TaiI ACGT 5 cut(s) 709, 1189, 1242, 1503, 1676
TatI WGTACW 3 cut(s) 29, 760, 1462
TauI GCSGC 3 cut(s) 609, 835, 1867
TfiI GAWTC 5 cut(s) 127, 227, 690, 1033, 1529
Tru1I TTAA 5 cut(s) 63, 147, 437, 677, 738
Tru9I TTAA 5 cut(s) 63, 147, 437, 677, 738
TscAI CASTG 4 cut(s) 589, 1084, 1234, 1573
TseFI GTSAC 2 cut(s) 1093, 1816
TseI GCWGC 8 cut(s) 239, 302, 537, 938, 1427, 1483, 1486, 1741
Tsp45I GTSAC 2 cut(s) 1093, 1816
TspDTI ATGAA 7 cut(s) 456, 1128, 1246, 1520, 1661, 1667, 1747
TspRI CASTG 4 cut(s) 589, 1084, 1234, 1573
VpaK11BI GGWCC 1 cut(s) 755
VspI ATTAAT 1 cut(s) 147
XapI RAATTY 2 cut(s) 308, 325
XceI RCATGY 3 cut(s) 58, 490, 1312
XmiI GTMKAC 2 cut(s) 1074, 1676
XmnI GAANNNNTTC 1 cut(s) 1275
XspI CTAG 3 cut(s) 102, 461, 974
ZraI GACGTC 1 cut(s) 1501
ZrmI AGTACT 2 cut(s) 31, 762
Zsp2I ATGCAT 1 cut(s) 433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.