Rh2BG206200

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0007199
Physical Location & Seq
Forward (+)
19067718 .. 19071897
4180 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG206200.1

Sequence Viewer

Length: 1521 bp
ATGCTTCGTTGCGTGTCAGAGTACCTTCTCATGACTGAGGAGTATGCAGTTGGAAACCTTGTGGGAAGAACTGATGCTTACCTGAATGAAGTGGCACTTAAAAGCCTTGCAGGCGCAGTTTCAGTTTTGCACATGTCAGAAAGCTTCCTCCCAATTGCGGAGAAAGTGAAGTTAGTGAGCCGATGCATTGATGCAATTGCTTACATTACCTGCAAAGACAGCCAGTTTTGTTTGTCTGGTAGGACTGATAGTGGCCATGAGAGTCTAAGCTCTTCAGCGGTGTATCAAACAAAACCTGTTGTTGATTGGTGGGCTGAAGATTTAACTGTCCTTAGAATTGATACCTTCCAAAGAGTTCTGATTGCAATGATGGCAAGAGGGTTTAAGCAATTCGCTCTTGGACCAATACTCATGCTCTATGCACAGAAATCTCTAAGAGGTTTGGAAACATTTGGAAAGGGAAGGAAGAAAATCGAGCCACGACAAGAGCATGAGAAGAGAGTTGTCTTGGAAACAATTGTGAGTCTTCTGCCAAGGGAGAAGAATACAATGTCAGTTAGCTTTCTTTCTATGCTGCTTCGTGCTGCAAAAAATCTAGAAACAACAGTTGCTTGTCAGCTAGATTTGGAGAAGAGAATGGGATTGCAATTGGCTCAGGCTGTCTTGGATGATCTCTTGATTCCCTCTTATTCCTTTAGCGGGGACACATTGCTTGATGTGGATACCGTGCAAAGGATTATGATGAATTACCTAGAGTATTATACGGATAGGAACCATTTGAGCTTCAGTGCAGATGATGAGTATGTTTCTCCTCCACTAAGTGACTTGGAACGAGTTGGAAAGTTAATGGAGAACTACCTTGCCGAAATAGCAACTGATCGGAACTTATCTGTTTCAAAGTTCATTGGCATTGCTGAACTTATTCCAGAACAGTCAAGGATAACCGAAGATGGGATGTACAGAGCAGTTGATATCTACCTAAAGGCACATCCTAGTCTAAGTGATATGGAGAAAAAGAAAGTCTGTAGTGTGATGGATTGCCAGAAGCTTTCGCGTGAGGCCTGTGCTCATGCTGCTCAGAATGACCGGCTTCCTGTTCAAACTGTTGTCCAAGTGCTCTACTACGAGCAGCAGCGCCTTCGAGATGTTATGGATGGTAGTCTCATTGGTGGTGAATCATCCGTACTTCCTTCCAAAGTAAACAACTTGTACTCCACTGATCTCCACCACCATGTTCCCGATGAGCTTTCCATGTTGCGTAGACAAAATGAGGAGCTGAAAATAGAGTTGCTGAAGATGAAAATGAAGCTGAAAGAAATTGAAACATCTACTATGAGGACAGTAACAAGCAGTCCAATGGGAAACATTTCAACATCTAGTGATAAGCCTCCTCTGCCTCGAAAATCATTTATCAACTCGGTTTCAAAAAGACTTGGGAAACTTTCTCCATTTGGAGTGACACCTCCCAATCCCAAAGCCCGTGCAAGACCTGCTAAAGATCGGCGGCATTCAATTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0042742 GO:0043207 GO:0050896 GO:0051704 GO:0051707 GO:0098542
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

506

Amino Acids

57.0

Weight (kDa)

8.26

Isoelectric Point (pI)

40.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 102 - 355 2.6e-89 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 218, 1498
AccI GTMKAC 1 cut(s) 1261
AccII CGCG 1 cut(s) 1054
AciI CCGC 4 cut(s) 158, 278, 699, 1504
AcoI YGGCCR 1 cut(s) 253
AcuI CTGAAG 4 cut(s) 258, 336, 769, 1313
AdeI CACNNNGTG 1 cut(s) 821
AfaI GTAC 4 cut(s) 23, 959, 1185, 1211
AfiI CCNNNNNNNGG 4 cut(s) 157, 699, 732, 951
AflIII ACRYGT 1 cut(s) 132
AgsI TTSAA 6 cut(s) 897, 1100, 1322, 1371, 1425, 1512
AluBI AGCT 9 cut(s) 144, 270, 561, 619, 783, 1048, 1246, 1276, 1309
AluI AGCT 9 cut(s) 144, 270, 561, 619, 783, 1048, 1246, 1276, 1309
Alw21I GWGCWC 2 cut(s) 1069, 1119
Alw26I GTCTC 1 cut(s) 1166
AoxI GGCC 2 cut(s) 253, 1059
ApeKI GCWGC 5 cut(s) 574, 584, 1073, 1129, 1132
Asp700I GAANNNNTTC 2 cut(s) 921, 1366
AspLEI GCGC 2 cut(s) 116, 1137
AspS9I GGNCC 1 cut(s) 401
AsuHPI GGTGA 1 cut(s) 1184
AvaII GGWCC 1 cut(s) 401
BalI TGGCCA 1 cut(s) 255
BbsI GAAGAC 1 cut(s) 518
Bbv12I GWGCWC 2 cut(s) 1069, 1119
BbvI GCAGC 5 cut(s) 561, 571, 1060, 1141, 1144
BccI CCATC 4 cut(s) 364, 944, 1027, 1148
BcgI CGANNNNNNTGC 2 cut(s) 1121, 1155
BciVI GTATCC 1 cut(s) 715
BcoDI GTCTC 1 cut(s) 1166
BfaI CTAG 5 cut(s) 596, 620, 752, 993, 1377
BfmI CTRYAG 1 cut(s) 1024
BfoI RGCGCY 1 cut(s) 1138
BfuAI ACCTGC 2 cut(s) 218, 1498
BfuI GTATCC 1 cut(s) 715
BglI GCCNNNNNGGC 1 cut(s) 111
BisI GCNGC 6 cut(s) 575, 585, 1074, 1130, 1133, 1505
BlsI GCNGC 6 cut(s) 576, 586, 1075, 1131, 1134, 1506
Bme18I GGWCC 1 cut(s) 401
BmgT120I GGNCC 1 cut(s) 401
BmiI GGNNCC 1 cut(s) 773
BmsI GCATC 3 cut(s) 64, 173, 181
BpiI GAAGAC 1 cut(s) 518
Bpu10I CCTNAGC 1 cut(s) 654
BsaJI CCNNGG 1 cut(s) 533
BsaXI ACNNNNNCTCC 2 cut(s) 1196, 1226
Bsc4I CCNNNNNNNGG 4 cut(s) 157, 699, 732, 951
Bse118I RCCGGY 1 cut(s) 1086
Bse1I ACTGG 1 cut(s) 223
Bse3DI GCAATG 3 cut(s) 372, 707, 909
BseDI CCNNGG 1 cut(s) 533
BseGI GGATG 5 cut(s) 673, 960, 988, 1159, 1178
BseLI CCNNNNNNNGG 4 cut(s) 157, 699, 732, 951
BseMI GCAATG 3 cut(s) 372, 707, 909
BseMII CTCAG 3 cut(s) 27, 668, 1091
BseNI ACTGG 1 cut(s) 223
BseRI GAGGAG 4 cut(s) 53, 801, 1286, 1380
BseXI GCAGC 5 cut(s) 561, 571, 1060, 1141, 1144
BsgI GTGCAG 1 cut(s) 810
Bsh1236I CGCG 1 cut(s) 1054
BshFI GGCC 2 cut(s) 255, 1061
BsiHKAI GWGCWC 2 cut(s) 1069, 1119
BsiSI CCGG 1 cut(s) 1087
BslFI GGGAC 1 cut(s) 716
BslI CCNNNNNNNGG 4 cut(s) 157, 699, 732, 951
BsmAI GTCTC 1 cut(s) 1166
BsmFI GGGAC 1 cut(s) 716
BsmI GAATGC 1 cut(s) 1507
BsnI GGCC 2 cut(s) 255, 1061
Bsp1286I GDGCHC 2 cut(s) 1069, 1119
Bsp1407I TGTACA 1 cut(s) 957
Bsp143I GATC 4 cut(s) 670, 877, 1219, 1498
BspACI CCGC 4 cut(s) 158, 278, 699, 1504
BspANI GGCC 2 cut(s) 255, 1061
BspCNI CTCAG 3 cut(s) 28, 667, 1090
BspFNI CGCG 1 cut(s) 1054
BspHI TCATGA 1 cut(s) 30
BspLI GGNNCC 1 cut(s) 773
BspMI ACCTGC 2 cut(s) 218, 1498
BspQI GCTCTTC 1 cut(s) 277
BsrDI GCAATG 3 cut(s) 372, 707, 909
BsrFI RCCGGY 1 cut(s) 1086
BsrGI TGTACA 1 cut(s) 957
BsrI ACTGG 1 cut(s) 223
BssAI RCCGGY 1 cut(s) 1086
BssECI CCNNGG 1 cut(s) 533
BssMI GATC 4 cut(s) 670, 877, 1219, 1498
BssT1I CCWWGG 1 cut(s) 533
Bst4CI ACNGT 6 cut(s) 328, 607, 727, 933, 1105, 1342
Bst6I CTCTTC 3 cut(s) 277, 491, 626
BstAPI GCANNNNNTGC 1 cut(s) 1490
BstAUI TGTACA 1 cut(s) 957
BstC8I GCNNGC 1 cut(s) 112
BstDEI CTNAG 8 cut(s) 36, 266, 332, 434, 654, 818, 998, 1077
BstF5I GGATG 5 cut(s) 673, 960, 988, 1159, 1178
BstFNI CGCG 1 cut(s) 1054
BstH2I RGCGCY 1 cut(s) 1138
BstHHI GCGC 2 cut(s) 116, 1137
BstKTI GATC 4 cut(s) 673, 880, 1222, 1501
BstMAI GTCTC 1 cut(s) 1166
BstMBI GATC 4 cut(s) 670, 877, 1219, 1498
BstMWI GCNNNNNNNGC 7 cut(s) 111, 219, 371, 869, 1073, 1393, 1490
BstNSI RCATGY 1 cut(s) 136
BstSFI CTRYAG 1 cut(s) 1024
BstUI CGCG 1 cut(s) 1054
BstV1I GCAGC 5 cut(s) 561, 571, 1060, 1141, 1144
BstV2I GAAGAC 1 cut(s) 518
BsuI GTATCC 1 cut(s) 715
BsuRI GGCC 2 cut(s) 255, 1061
BtsCI GGATG 5 cut(s) 673, 960, 988, 1159, 1178
BtsIMutI CAGTG 2 cut(s) 793, 1215
BveI ACCTGC 2 cut(s) 218, 1498
Cac8I GCNNGC 1 cut(s) 112
CciI TCATGA 1 cut(s) 30
CfoI GCGC 2 cut(s) 116, 1137
Cfr10I RCCGGY 1 cut(s) 1086
Cfr13I GGNCC 1 cut(s) 401
Csp6I GTAC 4 cut(s) 22, 958, 1184, 1210
CviAII CATG 8 cut(s) 31, 133, 257, 412, 491, 1070, 1232, 1252
CviQI GTAC 4 cut(s) 22, 958, 1184, 1210
DdeI CTNAG 8 cut(s) 36, 266, 332, 434, 654, 818, 998, 1077
DpnI GATC 4 cut(s) 672, 879, 1221, 1500
DpnII GATC 4 cut(s) 670, 877, 1219, 1498
DraIII CACNNNGTG 1 cut(s) 821
EaeI YGGCCR 1 cut(s) 253
Eam1104I CTCTTC 3 cut(s) 277, 491, 626
EarI CTCTTC 3 cut(s) 277, 491, 626
Eco130I CCWWGG 1 cut(s) 533
Eco147I AGGCCT 1 cut(s) 1061
Eco32I GATATC 1 cut(s) 973
Eco47I GGWCC 1 cut(s) 401
Eco57I CTGAAG 4 cut(s) 258, 336, 769, 1313
EcoRV GATATC 1 cut(s) 973
EcoT14I CCWWGG 1 cut(s) 533
EcoT22I ATGCAT 1 cut(s) 188
ErhI CCWWGG 1 cut(s) 533
FaeI CATG 8 cut(s) 34, 136, 260, 415, 494, 1073, 1235, 1255
FalI AAGNNNNNCTT 2 cut(s) 81, 113
FaqI GGGAC 1 cut(s) 716
FatI CATG 8 cut(s) 30, 132, 256, 411, 490, 1069, 1231, 1251
FauI CCCGC 1 cut(s) 692
FblI GTMKAC 1 cut(s) 1261
Fnu4HI GCNGC 6 cut(s) 575, 585, 1074, 1130, 1133, 1505
FokI GGATG 5 cut(s) 680, 967, 975, 1165, 1166
Fsp4HI GCNGC 6 cut(s) 575, 585, 1074, 1130, 1133, 1505
FspBI CTAG 5 cut(s) 596, 620, 752, 993, 1377
GlaI GCGC 2 cut(s) 115, 1136
GluI GCNGC 6 cut(s) 575, 585, 1074, 1130, 1133, 1505
HaeII RGCGCY 1 cut(s) 1138
HaeIII GGCC 2 cut(s) 255, 1061
HapII CCGG 1 cut(s) 1087
HhaI GCGC 2 cut(s) 116, 1137
Hin1II CATG 8 cut(s) 34, 136, 260, 415, 494, 1073, 1235, 1255
Hin6I GCGC 2 cut(s) 114, 1135
HinP1I GCGC 2 cut(s) 114, 1135
HindIII AAGCTT 2 cut(s) 142, 1046
HinfI GANTC 4 cut(s) 262, 523, 679, 1175
HpaII CCGG 1 cut(s) 1087
HphI GGTGA 1 cut(s) 1184
Hpy166II GTNNAC 2 cut(s) 1201, 1262
Hpy188I TCNGA 5 cut(s) 19, 139, 360, 882, 1080
Hpy188III TCNNGA 7 cut(s) 31, 596, 676, 926, 1142, 1238, 1518
Hpy8I GTNNAC 2 cut(s) 1201, 1262
HpyAV CCTTC 5 cut(s) 35, 355, 456, 1148, 1200
HpyCH4III ACNGT 6 cut(s) 328, 607, 727, 933, 1105, 1342
HpyF10VI GCNNNNNNNGC 7 cut(s) 111, 219, 371, 869, 1073, 1393, 1490
HpyF3I CTNAG 8 cut(s) 36, 266, 332, 434, 654, 818, 998, 1077
Hsp92II CATG 8 cut(s) 34, 136, 260, 415, 494, 1073, 1235, 1255
HspAI GCGC 2 cut(s) 114, 1135
Kzo9I GATC 4 cut(s) 670, 877, 1219, 1498
LguI GCTCTTC 1 cut(s) 277
LmnI GCTCC 1 cut(s) 1273
Lsp1109I GCAGC 5 cut(s) 561, 571, 1060, 1141, 1144
LweI GCATC 3 cut(s) 64, 173, 181
MaeI CTAG 5 cut(s) 596, 620, 752, 993, 1377
MaeIII GTNAC 3 cut(s) 821, 1342, 1456
MalI GATC 4 cut(s) 672, 879, 1221, 1500
MboI GATC 4 cut(s) 670, 877, 1219, 1498
MfeI CAATTG 4 cut(s) 153, 195, 516, 647
MhlI GDGCHC 2 cut(s) 1069, 1119
MlsI TGGCCA 1 cut(s) 255
MluCI AATT 9 cut(s) 153, 195, 336, 389, 516, 647, 745, 1317, 1512
MluNI TGGCCA 1 cut(s) 255
MlyI GAGTC 2 cut(s) 271, 532
MmeI TCCRAC 2 cut(s) 31, 817
Mox20I TGGCCA 1 cut(s) 255
Mph1103I ATGCAT 1 cut(s) 188
MroXI GAANNNNTTC 2 cut(s) 921, 1366
MscI TGGCCA 1 cut(s) 255
MseI TTAA 4 cut(s) 99, 323, 384, 845
MslI CAYNNNNRTG 1 cut(s) 1230
Msp20I TGGCCA 1 cut(s) 255
MspA1I CMGCKG 1 cut(s) 278
MspI CCGG 1 cut(s) 1087
MunI CAATTG 4 cut(s) 153, 195, 516, 647
Mva1269I GAATGC 1 cut(s) 1507
MvnI CGCG 1 cut(s) 1054
MwoI GCNNNNNNNGC 7 cut(s) 111, 219, 371, 869, 1073, 1393, 1490
NdeII GATC 4 cut(s) 670, 877, 1219, 1498
NlaIII CATG 8 cut(s) 34, 136, 260, 415, 494, 1073, 1235, 1255
NlaIV GGNNCC 1 cut(s) 773
NmuCI GTSAC 2 cut(s) 821, 1456
NsiI ATGCAT 1 cut(s) 188
NspI RCATGY 1 cut(s) 136
PagI TCATGA 1 cut(s) 30
PceI AGGCCT 1 cut(s) 1061
PciI ACATGT 1 cut(s) 132
PciSI GCTCTTC 1 cut(s) 277
PctI GAATGC 1 cut(s) 1507
PdmI GAANNNNTTC 2 cut(s) 921, 1366
PfeI GAWTC 2 cut(s) 679, 1175
PkrI GCNGC 6 cut(s) 576, 586, 1075, 1131, 1134, 1506
PleI GAGTC 2 cut(s) 270, 531
PpsI GAGTC 2 cut(s) 270, 531
PscI ACATGT 1 cut(s) 132
PspN4I GGNNCC 1 cut(s) 773
PspPI GGNCC 1 cut(s) 401
RsaI GTAC 4 cut(s) 23, 959, 1185, 1211
RsaNI GTAC 4 cut(s) 22, 958, 1184, 1210
RseI CAYNNNNRTG 1 cut(s) 1230
SapI GCTCTTC 1 cut(s) 277
SaqAI TTAA 4 cut(s) 99, 323, 384, 845
SatI GCNGC 6 cut(s) 575, 585, 1074, 1130, 1133, 1505
Sau3AI GATC 4 cut(s) 670, 877, 1219, 1498
Sau96I GGNCC 1 cut(s) 401
SchI GAGTC 2 cut(s) 271, 532
SduI GDGCHC 2 cut(s) 1069, 1119
SfaNI GCATC 3 cut(s) 64, 173, 181
SfcI CTRYAG 1 cut(s) 1024
SinI GGWCC 1 cut(s) 401
SmiMI CAYNNNNRTG 1 cut(s) 1230
Sse9I AATT 9 cut(s) 153, 195, 336, 389, 516, 647, 745, 1317, 1512
SseBI AGGCCT 1 cut(s) 1061
SsiI CCGC 4 cut(s) 158, 278, 699, 1504
SspMI CTAG 5 cut(s) 596, 620, 752, 993, 1377
StuI AGGCCT 1 cut(s) 1061
StyI CCWWGG 1 cut(s) 533
TaaI ACNGT 6 cut(s) 328, 607, 727, 933, 1105, 1342
TaqI TCGA 3 cut(s) 474, 1141, 1399
TasI AATT 9 cut(s) 153, 195, 336, 389, 516, 647, 745, 1317, 1512
TatI WGTACW 2 cut(s) 957, 1209
TauI GCSGC 1 cut(s) 1507
TfiI GAWTC 2 cut(s) 679, 1175
Tru1I TTAA 4 cut(s) 99, 323, 384, 845
Tru9I TTAA 4 cut(s) 99, 323, 384, 845
TscAI CASTG 2 cut(s) 793, 1222
TseFI GTSAC 2 cut(s) 821, 1456
TseI GCWGC 5 cut(s) 574, 584, 1073, 1129, 1132
Tsp45I GTSAC 2 cut(s) 821, 1456
TspDTI ATGAA 5 cut(s) 102, 758, 892, 1313, 1319
TspGWI ACGGA 2 cut(s) 779, 1171
TspRI CASTG 2 cut(s) 793, 1222
VpaK11BI GGWCC 1 cut(s) 401
XbaI TCTAGA 1 cut(s) 595
XceI RCATGY 1 cut(s) 136
XmiI GTMKAC 1 cut(s) 1261
XmnI GAANNNNTTC 2 cut(s) 921, 1366
XspI CTAG 5 cut(s) 596, 620, 752, 993, 1377
Zsp2I ATGCAT 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.