ATCG00130

ATP synthase B/B' CF(0)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
Pt
OG0010725
Physical Location & Seq
Reverse (-)
11529 .. 12798
1270 bp
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UTR
Exon/CDS
Intron
ATCG00130.1

Sequence Viewer

Length: 555 bp
ATGAAAAATTTAACCGATTCTTTCGTTTACTTGGGTCACTGGCCATCCGCCGGGAGTTTCGGATTTAATACCGATATTTTAGCAACAAATCCAATAAATCTAAGTGTAGTTTTCGGTGTATTGATCTTTTTTGGAAAGGGAGTGTTAAATGATTTATTAGATAACCGAAAGCAGAGGATATTAAATACTATTCGAAATTCAGAAGAACTGCGTGAAGGAGCTATTCAACAATTAGAAAATGCCCGGGCGCGCTTGCGTAACGTAGAAACGGAAGCGGATAAGTTTCGCGTGAATGGATACTCTGAAATCGAACGAGAAAAATTGAATTTGATTAATTCAACTTATAAAACTTTGAAACAATTAGAAAATTACAAAAATGAAACCATTCTTTTTGAGCAACAAAGAACAATTAATCAAGTCCGCGAACGGGTTTTCCAACAAGCTTTACAAGGAGCTATAGGAACCCTAAATAGTTGTTTGAGTAATGAGTTACATTTACGTACTATTAATGCAAATATTGGGATGTTTGGTACGATGAAAGAAATAACTGATTAA

Protein Analysis

184

Amino Acids

21.06

Weight (kDa)

7.85

Isoelectric Point (pI)

33.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP-synt_B PF00430 26 - 156 2.9e-31 ATP synthase B/B' CF(0)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 345
AccII CGCG 3 cut(s) 250, 288, 423
AciI CCGC 3 cut(s) 48, 275, 421
AcoI YGGCCR 1 cut(s) 41
AcsI RAATTY 3 cut(s) 7, 196, 325
AfaI GTAC 2 cut(s) 502, 532
AfiI CCNNNNNNNGG 2 cut(s) 50, 427
AgsI TTSAA 4 cut(s) 227, 325, 339, 355
AloI GAACNNNNNNTCC 2 cut(s) 417, 449
AluBI AGCT 3 cut(s) 221, 443, 455
AluI AGCT 3 cut(s) 221, 443, 455
Ama87I CYCGRG 1 cut(s) 243
AoxI GGCC 1 cut(s) 41
ApoI RAATTY 3 cut(s) 7, 196, 325
AseI ATTAAT 3 cut(s) 333, 411, 507
Asp700I GAANNNNTTC 1 cut(s) 384
AspLEI GCGC 2 cut(s) 250, 252
AsuC2I CCSGG 3 cut(s) 52, 244, 245
AsuII TTCGAA 1 cut(s) 193
AvaI CYCGRG 1 cut(s) 243
BalI TGGCCA 1 cut(s) 43
BccI CCATC 1 cut(s) 52
BciVI GTATCC 1 cut(s) 290
BcnI CCSGG 3 cut(s) 52, 244, 245
BfmI CTRYAG 1 cut(s) 456
BfuI GTATCC 1 cut(s) 290
Bme1390I CCNGG 3 cut(s) 52, 244, 245
BmeT110I CYCGRG 1 cut(s) 243
BmiI GGNNCC 1 cut(s) 463
BmrFI CCNGG 3 cut(s) 52, 244, 245
Bpu14I TTCGAA 1 cut(s) 193
BpuMI CCSGG 3 cut(s) 52, 244, 245
BsaAI YACGTR 1 cut(s) 500
BsaJI CCNNGG 1 cut(s) 243
Bsc4I CCNNNNNNNGG 2 cut(s) 50, 427
Bse1I ACTGG 1 cut(s) 44
BseDI CCNNGG 1 cut(s) 243
BseGI GGATG 2 cut(s) 44, 528
BseLI CCNNNNNNNGG 2 cut(s) 50, 427
BseNI ACTGG 1 cut(s) 44
BsePI GCGCGC 1 cut(s) 248
Bsh1236I CGCG 3 cut(s) 250, 288, 423
BshFI GGCC 1 cut(s) 43
BsiHKCI CYCGRG 1 cut(s) 243
BsiSI CCGG 2 cut(s) 51, 244
BslI CCNNNNNNNGG 2 cut(s) 50, 427
BsnI GGCC 1 cut(s) 43
BsoBI CYCGRG 1 cut(s) 243
Bsp119I TTCGAA 1 cut(s) 193
Bsp143I GATC 1 cut(s) 123
BspACI CCGC 3 cut(s) 48, 275, 421
BspANI GGCC 1 cut(s) 43
BspFNI CGCG 3 cut(s) 250, 288, 423
BspLI GGNNCC 1 cut(s) 463
BspT104I TTCGAA 1 cut(s) 193
BsrI ACTGG 1 cut(s) 44
BssECI CCNNGG 1 cut(s) 243
BssHII GCGCGC 1 cut(s) 248
BssMI GATC 1 cut(s) 123
BstBAI YACGTR 1 cut(s) 500
BstBI TTCGAA 1 cut(s) 193
BstC8I GCNNGC 2 cut(s) 250, 254
BstDEI CTNAG 1 cut(s) 101
BstF5I GGATG 2 cut(s) 44, 528
BstFNI CGCG 3 cut(s) 250, 288, 423
BstHHI GCGC 2 cut(s) 250, 252
BstKTI GATC 1 cut(s) 126
BstMBI GATC 1 cut(s) 123
BstSCI CCNGG 3 cut(s) 50, 242, 243
BstSFI CTRYAG 1 cut(s) 456
BstSNI TACGTA 1 cut(s) 500
BstUI CGCG 3 cut(s) 250, 288, 423
BsuI GTATCC 1 cut(s) 290
BsuRI GGCC 1 cut(s) 43
BtsCI GGATG 2 cut(s) 44, 528
BtsIMutI CAGTG 1 cut(s) 37
Cac8I GCNNGC 2 cut(s) 250, 254
CfoI GCGC 2 cut(s) 250, 252
Cfr9I CCCGGG 1 cut(s) 243
Csp6I GTAC 2 cut(s) 501, 531
CviJI RGCY 4 cut(s) 43, 221, 443, 455
CviKI_1 RGCY 4 cut(s) 43, 221, 443, 455
CviQI GTAC 2 cut(s) 501, 531
DdeI CTNAG 1 cut(s) 101
DpnI GATC 1 cut(s) 125
DpnII GATC 1 cut(s) 123
EaeI YGGCCR 1 cut(s) 41
EciI GGCGGA 1 cut(s) 37
Eco105I TACGTA 1 cut(s) 500
Eco88I CYCGRG 1 cut(s) 243
FaiI YATR 2 cut(s) 345, 458
FokI GGATG 2 cut(s) 31, 535
GlaI GCGC 2 cut(s) 249, 251
HaeIII GGCC 1 cut(s) 43
HapII CCGG 2 cut(s) 51, 244
HhaI GCGC 2 cut(s) 250, 252
Hin6I GCGC 2 cut(s) 248, 250
HinP1I GCGC 2 cut(s) 248, 250
HindIII AAGCTT 1 cut(s) 441
HinfI GANTC 1 cut(s) 17
HpaII CCGG 2 cut(s) 51, 244
Hpy166II GTNNAC 1 cut(s) 28
Hpy188I TCNGA 3 cut(s) 62, 202, 304
Hpy8I GTNNAC 1 cut(s) 28
HpyAV CCTTC 1 cut(s) 209
HpyCH4IV ACGT 2 cut(s) 261, 499
HpyCH4V TGCA 1 cut(s) 512
HpyF3I CTNAG 1 cut(s) 101
HpySE526I ACGT 2 cut(s) 261, 499
HspAI GCGC 2 cut(s) 248, 250
Kzo9I GATC 1 cut(s) 123
LmnI GCTCC 2 cut(s) 218, 452
LpnPI CCDG 3 cut(s) 25, 64, 257
MaeII ACGT 2 cut(s) 261, 499
MaeIII GTNAC 3 cut(s) 35, 257, 489
MalI GATC 1 cut(s) 125
MboI GATC 1 cut(s) 123
MboII GAAGA 1 cut(s) 215
MlsI TGGCCA 1 cut(s) 43
MluCI AATT 9 cut(s) 7, 196, 230, 320, 325, 334, 359, 367, 408
MluNI TGGCCA 1 cut(s) 43
MmeI TCCRAC 1 cut(s) 460
MnlI CCTC 1 cut(s) 168
Mox20I TGGCCA 1 cut(s) 43
MroXI GAANNNNTTC 1 cut(s) 384
MscI TGGCCA 1 cut(s) 43
MseI TTAA 8 cut(s) 11, 66, 146, 182, 333, 411, 507, 553
Msp20I TGGCCA 1 cut(s) 43
MspI CCGG 2 cut(s) 51, 244
MspR9I CCNGG 3 cut(s) 52, 244, 245
MvnI CGCG 3 cut(s) 250, 288, 423
NciI CCSGG 3 cut(s) 52, 244, 245
NdeII GATC 1 cut(s) 123
NlaIV GGNNCC 1 cut(s) 463
NmuCI GTSAC 1 cut(s) 35
NspV TTCGAA 1 cut(s) 193
PauI GCGCGC 1 cut(s) 248
PdmI GAANNNNTTC 1 cut(s) 384
PfeI GAWTC 1 cut(s) 17
Ppu21I YACGTR 1 cut(s) 500
PshBI ATTAAT 3 cut(s) 333, 411, 507
PsiI TTATAA 1 cut(s) 345
PspN4I GGNNCC 1 cut(s) 463
PteI GCGCGC 1 cut(s) 248
RsaI GTAC 2 cut(s) 502, 532
RsaNI GTAC 2 cut(s) 501, 531
SaqAI TTAA 8 cut(s) 11, 66, 146, 182, 333, 411, 507, 553
Sau3AI GATC 1 cut(s) 123
ScrFI CCNGG 3 cut(s) 52, 244, 245
SetI ASST 5 cut(s) 223, 264, 445, 457, 502
SfcI CTRYAG 1 cut(s) 456
SfuI TTCGAA 1 cut(s) 193
SmaI CCCGGG 1 cut(s) 245
SnaBI TACGTA 1 cut(s) 500
SrfI GCCCGGGC 1 cut(s) 245
Sse9I AATT 9 cut(s) 7, 196, 230, 320, 325, 334, 359, 367, 408
SsiI CCGC 3 cut(s) 48, 275, 421
SspI AATATT 1 cut(s) 517
StyD4I CCNGG 3 cut(s) 50, 242, 243
TaiI ACGT 2 cut(s) 264, 502
TaqI TCGA 2 cut(s) 193, 309
TasI AATT 9 cut(s) 7, 196, 230, 320, 325, 334, 359, 367, 408
TfiI GAWTC 1 cut(s) 17
Tru1I TTAA 8 cut(s) 11, 66, 146, 182, 333, 411, 507, 553
Tru9I TTAA 8 cut(s) 11, 66, 146, 182, 333, 411, 507, 553
TscAI CASTG 1 cut(s) 44
TseFI GTSAC 1 cut(s) 35
Tsp45I GTSAC 1 cut(s) 35
TspDTI ATGAA 3 cut(s) 17, 393, 551
TspGWI ACGGA 1 cut(s) 284
TspMI CCCGGG 1 cut(s) 243
TspRI CASTG 1 cut(s) 44
VspI ATTAAT 3 cut(s) 333, 411, 507
XapI RAATTY 3 cut(s) 7, 196, 325
XmaI CCCGGG 1 cut(s) 243
XmnI GAANNNNTTC 1 cut(s) 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.