MD15G1134000.v1.1

F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010725
Physical Location & Seq
Forward (+)
9743184 .. 9743429
246 bp
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UTR
Exon/CDS
Intron
MD15G1134000.v1.1.491

Sequence Viewer

Length: 246 bp
ATGAATCCATTGATTTATGCCGCTTCCGTTATTGCTACTGGGTTGGCTGTCGGGCTTGCTTCTATTGGACCTGGGGTTGGTCAAGGTACTGTTGCGGGACAGGCTGTAGAAGGGATCGCGAGACAACCAGAGGCGGAGGGAAAAATCCGAGGTACTTTATTGCTTAGTCTAGCTTTTATGGAAGCTTTAACAATTTATGGACTGGTTGTCGCATTAGCTCTTTTATTTGCGAATCCCTTTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006754 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0008152 GO:0008324 GO:0009058 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009167 GO:0009168 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0009987 GO:0015075 GO:0015077 GO:0015078 GO:0015318 GO:0015399 GO:0015405 GO:0015672 GO:0015985 GO:0015986 GO:0016020 GO:0016462 GO:0016469 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0031976 GO:0031984 GO:0032991 GO:0033177 GO:0034220 GO:0034357 GO:0034641 GO:0034654 GO:0042623 GO:0042625 GO:0042626 GO:0042651 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0044769 GO:0045259 GO:0045263 GO:0046034 GO:0046390 GO:0046483 GO:0046933 GO:0051179 GO:0051234 GO:0055035 GO:0055085 GO:0055086 GO:0071704 GO:0072521 GO:0072522 GO:0090407 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0098796 GO:0099131 GO:0099132 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1902600
KEGG Pathways
Metabolic & Signaling
ko00190 ko00195 ko01100 map00190 map00195 map01100
Pfam Domains
Protein Families
ATP-synt_C

Protein Analysis

82

Amino Acids

8.12

Weight (kDa)

4.94

Isoelectric Point (pI)

10.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP-synt_C PF00137 11 - 73 3.3e-19 ATP synthase subunit C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 119
AciI CCGC 3 cut(s) 21, 95, 134
AclWI GGATC 1 cut(s) 122
AfaI GTAC 2 cut(s) 88, 154
AfiI CCNNNNNNNGG 1 cut(s) 77
AhdI GACNNNNNGTC 1 cut(s) 206
AjnI CCWGG 1 cut(s) 70
AluBI AGCT 3 cut(s) 173, 185, 218
AluI AGCT 3 cut(s) 173, 185, 218
Alw26I GTCTC 1 cut(s) 115
AlwI GGATC 1 cut(s) 122
AspS9I GGNCC 1 cut(s) 68
AvaII GGWCC 1 cut(s) 68
BciT130I CCWGG 1 cut(s) 72
BcoDI GTCTC 1 cut(s) 115
BfaI CTAG 1 cut(s) 170
BfmI CTRYAG 1 cut(s) 105
BisI GCNGC 1 cut(s) 21
BlsI GCNGC 1 cut(s) 22
Bme1390I CCNGG 1 cut(s) 72
Bme18I GGWCC 1 cut(s) 68
BmeRI GACNNNNNGTC 1 cut(s) 206
BmgT120I GGNCC 1 cut(s) 68
BmrFI CCNGG 1 cut(s) 72
BmrI ACTGGG 1 cut(s) 48
BmuI ACTGGG 1 cut(s) 48
BsaJI CCNNGG 2 cut(s) 71, 148
Bsc4I CCNNNNNNNGG 1 cut(s) 77
Bse1I ACTGG 2 cut(s) 43, 207
BseBI CCWGG 1 cut(s) 72
BseDI CCNNGG 2 cut(s) 71, 148
BseLI CCNNNNNNNGG 1 cut(s) 77
BseNI ACTGG 2 cut(s) 43, 207
Bsh1236I CGCG 1 cut(s) 119
BslFI GGGAC 1 cut(s) 111
BslI CCNNNNNNNGG 1 cut(s) 77
BsmAI GTCTC 1 cut(s) 115
BsmFI GGGAC 1 cut(s) 111
Bsp143I GATC 1 cut(s) 114
Bsp68I TCGCGA 1 cut(s) 119
BspACI CCGC 3 cut(s) 21, 95, 134
BspFNI CGCG 1 cut(s) 119
BspPI GGATC 1 cut(s) 122
BsrI ACTGG 2 cut(s) 43, 207
BssECI CCNNGG 2 cut(s) 71, 148
BssMI GATC 1 cut(s) 114
Bst2UI CCWGG 1 cut(s) 72
Bst4CI ACNGT 1 cut(s) 91
BstC8I GCNNGC 1 cut(s) 57
BstDEI CTNAG 1 cut(s) 164
BstFNI CGCG 1 cut(s) 119
BstKTI GATC 1 cut(s) 117
BstMAI GTCTC 1 cut(s) 115
BstMBI GATC 1 cut(s) 114
BstMWI GCNNNNNNNGC 1 cut(s) 101
BstNI CCWGG 1 cut(s) 72
BstSCI CCNGG 1 cut(s) 70
BstSFI CTRYAG 1 cut(s) 105
BstUI CGCG 1 cut(s) 119
BtuMI TCGCGA 1 cut(s) 119
Cac8I GCNNGC 1 cut(s) 57
Cfr13I GGNCC 1 cut(s) 68
Csp6I GTAC 2 cut(s) 87, 153
CviJI RGCY 6 cut(s) 47, 55, 104, 173, 185, 218
CviKI_1 RGCY 6 cut(s) 47, 55, 104, 173, 185, 218
CviQI GTAC 2 cut(s) 87, 153
DdeI CTNAG 1 cut(s) 164
DpnI GATC 1 cut(s) 116
DpnII GATC 1 cut(s) 114
DriI GACNNNNNGTC 1 cut(s) 206
Eam1105I GACNNNNNGTC 1 cut(s) 206
EciI GGCGGA 1 cut(s) 149
Eco47I GGWCC 1 cut(s) 68
EcoRII CCWGG 1 cut(s) 70
FaiI YATR 3 cut(s) 18, 179, 198
FaqI GGGAC 1 cut(s) 111
FauI CCCGC 1 cut(s) 88
Fnu4HI GCNGC 1 cut(s) 21
Fsp4HI GCNGC 1 cut(s) 21
FspBI CTAG 1 cut(s) 170
GluI GCNGC 1 cut(s) 21
HindIII AAGCTT 1 cut(s) 183
HinfI GANTC 2 cut(s) 4, 232
Hpy188I TCNGA 1 cut(s) 149
Hpy188III TCNNGA 1 cut(s) 118
HpyAV CCTTC 1 cut(s) 104
HpyCH4III ACNGT 1 cut(s) 91
HpyF10VI GCNNNNNNNGC 1 cut(s) 101
HpyF3I CTNAG 1 cut(s) 164
Kzo9I GATC 1 cut(s) 114
LpnPI CCDG 6 cut(s) 24, 57, 84, 86, 141, 188
MaeI CTAG 1 cut(s) 170
MalI GATC 1 cut(s) 116
MboI GATC 1 cut(s) 114
MluCI AATT 1 cut(s) 192
MnlI CCTC 3 cut(s) 124, 130, 143
MseI TTAA 2 cut(s) 188, 244
MspR9I CCNGG 1 cut(s) 72
MvaI CCWGG 1 cut(s) 72
MvnI CGCG 1 cut(s) 119
MwoI GCNNNNNNNGC 1 cut(s) 101
NdeII GATC 1 cut(s) 114
NruI TCGCGA 1 cut(s) 119
PfeI GAWTC 2 cut(s) 4, 232
PkrI GCNGC 1 cut(s) 22
Psp6I CCWGG 1 cut(s) 70
PspGI CCWGG 1 cut(s) 70
PspPI GGNCC 1 cut(s) 68
RruI TCGCGA 1 cut(s) 119
RsaI GTAC 2 cut(s) 88, 154
RsaNI GTAC 2 cut(s) 87, 153
SaqAI TTAA 2 cut(s) 188, 244
SatI GCNGC 1 cut(s) 21
Sau3AI GATC 1 cut(s) 114
Sau96I GGNCC 1 cut(s) 68
ScrFI CCNGG 1 cut(s) 72
SetI ASST 6 cut(s) 73, 88, 154, 175, 187, 220
SfcI CTRYAG 1 cut(s) 105
SinI GGWCC 1 cut(s) 68
Sse9I AATT 1 cut(s) 192
SsiI CCGC 3 cut(s) 21, 95, 134
SspMI CTAG 1 cut(s) 170
StyD4I CCNGG 1 cut(s) 70
TaaI ACNGT 1 cut(s) 91
TasI AATT 1 cut(s) 192
TauI GCSGC 1 cut(s) 23
TfiI GAWTC 2 cut(s) 4, 232
Tru1I TTAA 2 cut(s) 188, 244
Tru9I TTAA 2 cut(s) 188, 244
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 16
VpaK11BI GGWCC 1 cut(s) 68
XspI CTAG 1 cut(s) 170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.