RCHIOBHM_CPG0502451

ATP synthase B/B' CF(0)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
Pt
OG0010725
Physical Location & Seq
Forward (+)
99967 .. 100915
949 bp
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UTR
Exon/CDS
Intron
PRQ15720

Sequence Viewer

Length: 555 bp
ATGAAAAATGTAACCGATTCTTTCCTTTCCTTGGGTTATTGGCCATCCGCCGGGAGTTTCGGGTTTAATACCGATATTTTTGCAACAAATCTAATAAATCTAAGCGTAGTGCTTGGTGTGTTGATTTTTTTTGGAAAGGGGGTGTTAAGTGATTTATTAGATAATCGAAAACAGAGGATCTTGAAGACTATTCAAAATTCAGAAGAATTACGCGAGGCGGCCCTAGACCAGTTGGAGAAAGCCCGGGCCCGCTTACGGAAAGTAGAAATAGAAGCGGATCAGTTTCGAATGACTGGATACTCTGAAATAGAACGAGAAAAATTGAATTTGATTAATGCAACTTCTAAGACTTTGGAACAGTTAGAAAATTACAAAAATGAAACCATTCATTTTGAACAACAAAGAGCAATTAATCAAGTTCGACAACGGGTTTTTCAACAAGCCTTACAGGGGGCTCTAGGAACTCTGAATAGTTGTTTGAACAACGAATTACATTTACGTACCATCAGTACTAATATTGGCATGTTTGGAACGGTGAAAGAAATAACGGATTAG

Protein Analysis

184

Amino Acids

20.94

Weight (kDa)

6.76

Isoelectric Point (pI)

31.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP-synt_B PF00430 26 - 156 2.1e-33 ATP synthase B/B' CF(0)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 213
AciI CCGC 4 cut(s) 48, 218, 250, 275
AclWI GGATC 2 cut(s) 185, 285
AcoI YGGCCR 1 cut(s) 41
AcsI RAATTY 2 cut(s) 196, 325
AfaI GTAC 2 cut(s) 502, 511
AfiI CCNNNNNNNGG 4 cut(s) 31, 50, 255, 450
AgsI TTSAA 6 cut(s) 184, 194, 325, 395, 437, 481
AlwI GGATC 2 cut(s) 185, 285
Ama87I CYCGRG 1 cut(s) 243
AoxI GGCC 3 cut(s) 41, 219, 246
ApaI GGGCCC 1 cut(s) 250
ApoI RAATTY 2 cut(s) 196, 325
AseI ATTAAT 2 cut(s) 333, 411
Asp700I GAANNNNTTC 1 cut(s) 384
AspS9I GGNCC 3 cut(s) 220, 246, 247
AsuC2I CCSGG 3 cut(s) 52, 244, 245
AsuHPI GGTGA 1 cut(s) 547
AsuII TTCGAA 1 cut(s) 286
AvaI CYCGRG 1 cut(s) 243
BaeGI GKGCMC 1 cut(s) 250
BalI TGGCCA 1 cut(s) 43
BanII GRGCYC 2 cut(s) 250, 457
BbsI GAAGAC 1 cut(s) 191
BccI CCATC 2 cut(s) 52, 512
BcgI CGANNNNNNTGC 2 cut(s) 62, 96
BciVI GTATCC 1 cut(s) 290
BcnI CCSGG 3 cut(s) 52, 244, 245
BfaI CTAG 2 cut(s) 224, 458
BfuI GTATCC 1 cut(s) 290
BisI GCNGC 1 cut(s) 219
BlsI GCNGC 1 cut(s) 220
BmcAI AGTACT 1 cut(s) 511
Bme1390I CCNGG 3 cut(s) 52, 244, 245
BmeT110I CYCGRG 1 cut(s) 243
BmgT120I GGNCC 3 cut(s) 220, 246, 247
BmiI GGNNCC 1 cut(s) 248
BmrFI CCNGG 3 cut(s) 52, 244, 245
BpiI GAAGAC 1 cut(s) 191
Bpu14I TTCGAA 1 cut(s) 286
BpuMI CCSGG 3 cut(s) 52, 244, 245
BsaAI YACGTR 1 cut(s) 500
BsaJI CCNNGG 2 cut(s) 30, 243
Bsc4I CCNNNNNNNGG 4 cut(s) 31, 50, 255, 450
Bse1I ACTGG 2 cut(s) 229, 298
BseDI CCNNGG 2 cut(s) 30, 243
BseGI GGATG 1 cut(s) 44
BseLI CCNNNNNNNGG 4 cut(s) 31, 50, 255, 450
BseNI ACTGG 2 cut(s) 229, 298
BseSI GKGCMC 1 cut(s) 250
Bsh1236I CGCG 1 cut(s) 213
BshFI GGCC 3 cut(s) 43, 221, 248
BsiHKCI CYCGRG 1 cut(s) 243
BsiSI CCGG 2 cut(s) 51, 244
BslI CCNNNNNNNGG 4 cut(s) 31, 50, 255, 450
BsnI GGCC 3 cut(s) 43, 221, 248
BsoBI CYCGRG 1 cut(s) 243
Bsp119I TTCGAA 1 cut(s) 286
Bsp120I GGGCCC 1 cut(s) 246
Bsp1286I GDGCHC 2 cut(s) 250, 457
Bsp143I GATC 2 cut(s) 177, 277
BspACI CCGC 4 cut(s) 48, 218, 250, 275
BspANI GGCC 3 cut(s) 43, 221, 248
BspFNI CGCG 1 cut(s) 213
BspLI GGNNCC 1 cut(s) 248
BspPI GGATC 2 cut(s) 185, 285
BspT104I TTCGAA 1 cut(s) 286
BsrI ACTGG 2 cut(s) 229, 298
BssECI CCNNGG 2 cut(s) 30, 243
BssMI GATC 2 cut(s) 177, 277
BssT1I CCWWGG 1 cut(s) 30
Bst4CI ACNGT 2 cut(s) 360, 535
BstBAI YACGTR 1 cut(s) 500
BstBI TTCGAA 1 cut(s) 286
BstC8I GCNNGC 1 cut(s) 250
BstDEI CTNAG 2 cut(s) 101, 345
BstF5I GGATG 1 cut(s) 44
BstFNI CGCG 1 cut(s) 213
BstKTI GATC 2 cut(s) 180, 280
BstMBI GATC 2 cut(s) 177, 277
BstNSI RCATGY 1 cut(s) 526
BstSCI CCNGG 3 cut(s) 50, 242, 243
BstSLI GKGCMC 1 cut(s) 250
BstSNI TACGTA 1 cut(s) 500
BstUI CGCG 1 cut(s) 213
BstV2I GAAGAC 1 cut(s) 191
BstX2I RGATCY 1 cut(s) 177
BstYI RGATCY 1 cut(s) 177
BsuI GTATCC 1 cut(s) 290
BsuRI GGCC 3 cut(s) 43, 221, 248
BtsCI GGATG 1 cut(s) 44
Cac8I GCNNGC 1 cut(s) 250
Cfr13I GGNCC 3 cut(s) 220, 246, 247
Cfr9I CCCGGG 1 cut(s) 243
Csp6I GTAC 2 cut(s) 501, 510
CviAII CATG 1 cut(s) 523
CviJI RGCY 6 cut(s) 43, 221, 242, 248, 443, 455
CviKI_1 RGCY 6 cut(s) 43, 221, 242, 248, 443, 455
CviQI GTAC 2 cut(s) 501, 510
DdeI CTNAG 2 cut(s) 101, 345
DpnI GATC 2 cut(s) 179, 279
DpnII GATC 2 cut(s) 177, 277
EaeI YGGCCR 1 cut(s) 41
EciI GGCGGA 1 cut(s) 37
Eco105I TACGTA 1 cut(s) 500
Eco130I CCWWGG 1 cut(s) 30
Eco24I GRGCYC 2 cut(s) 250, 457
Eco88I CYCGRG 1 cut(s) 243
EcoT14I CCWWGG 1 cut(s) 30
EcoT38I GRGCYC 2 cut(s) 250, 457
ErhI CCWWGG 1 cut(s) 30
FaeI CATG 1 cut(s) 526
FaiI YATR 1 cut(s) 524
FatI CATG 1 cut(s) 522
FauI CCCGC 1 cut(s) 257
Fnu4HI GCNGC 1 cut(s) 219
FokI GGATG 1 cut(s) 31
FriOI GRGCYC 2 cut(s) 250, 457
Fsp4HI GCNGC 1 cut(s) 219
FspBI CTAG 2 cut(s) 224, 458
GluI GCNGC 1 cut(s) 219
HaeIII GGCC 3 cut(s) 43, 221, 248
HapII CCGG 2 cut(s) 51, 244
Hin1II CATG 1 cut(s) 526
HinfI GANTC 1 cut(s) 17
HpaII CCGG 2 cut(s) 51, 244
HphI GGTGA 1 cut(s) 547
Hpy188I TCNGA 3 cut(s) 202, 304, 468
Hpy188III TCNNGA 1 cut(s) 181
HpyCH4III ACNGT 2 cut(s) 360, 535
HpyCH4IV ACGT 1 cut(s) 499
HpyCH4V TGCA 2 cut(s) 83, 338
HpyF3I CTNAG 2 cut(s) 101, 345
HpySE526I ACGT 1 cut(s) 499
Hsp92II CATG 1 cut(s) 526
Kzo9I GATC 2 cut(s) 177, 277
LpnPI CCDG 5 cut(s) 64, 242, 257, 279, 434
MaeI CTAG 2 cut(s) 224, 458
MaeII ACGT 1 cut(s) 499
MaeIII GTNAC 1 cut(s) 10
MalI GATC 2 cut(s) 179, 279
MboI GATC 2 cut(s) 177, 277
MboII GAAGA 2 cut(s) 196, 215
MflI RGATCY 1 cut(s) 177
MhlI GDGCHC 2 cut(s) 250, 457
MlsI TGGCCA 1 cut(s) 43
MluCI AATT 7 cut(s) 196, 206, 320, 325, 367, 408, 488
MluNI TGGCCA 1 cut(s) 43
MmeI TCCRAC 1 cut(s) 213
MnlI CCTC 2 cut(s) 168, 208
Mox20I TGGCCA 1 cut(s) 43
MroXI GAANNNNTTC 1 cut(s) 384
MscI TGGCCA 1 cut(s) 43
MseI TTAA 4 cut(s) 66, 146, 333, 411
Msp20I TGGCCA 1 cut(s) 43
MspI CCGG 2 cut(s) 51, 244
MspR9I CCNGG 3 cut(s) 52, 244, 245
MvnI CGCG 1 cut(s) 213
NciI CCSGG 3 cut(s) 52, 244, 245
NdeII GATC 2 cut(s) 177, 277
NlaIII CATG 1 cut(s) 526
NlaIV GGNNCC 1 cut(s) 248
NspI RCATGY 1 cut(s) 526
NspV TTCGAA 1 cut(s) 286
PdmI GAANNNNTTC 1 cut(s) 384
PfeI GAWTC 1 cut(s) 17
PkrI GCNGC 1 cut(s) 220
Ppu21I YACGTR 1 cut(s) 500
PshBI ATTAAT 2 cut(s) 333, 411
PspN4I GGNNCC 1 cut(s) 248
PspOMI GGGCCC 1 cut(s) 246
PspPI GGNCC 3 cut(s) 220, 246, 247
PsuI RGATCY 1 cut(s) 177
RsaI GTAC 2 cut(s) 502, 511
RsaNI GTAC 2 cut(s) 501, 510
SaqAI TTAA 4 cut(s) 66, 146, 333, 411
SatI GCNGC 1 cut(s) 219
Sau3AI GATC 2 cut(s) 177, 277
Sau96I GGNCC 3 cut(s) 220, 246, 247
ScaI AGTACT 1 cut(s) 511
ScrFI CCNGG 3 cut(s) 52, 244, 245
SduI GDGCHC 2 cut(s) 250, 457
SetI ASST 1 cut(s) 502
SfuI TTCGAA 1 cut(s) 286
SmaI CCCGGG 1 cut(s) 245
SnaBI TACGTA 1 cut(s) 500
SrfI GCCCGGGC 1 cut(s) 245
Sse9I AATT 7 cut(s) 196, 206, 320, 325, 367, 408, 488
SsiI CCGC 4 cut(s) 48, 218, 250, 275
SspI AATATT 1 cut(s) 517
SspMI CTAG 2 cut(s) 224, 458
StyD4I CCNGG 3 cut(s) 50, 242, 243
StyI CCWWGG 1 cut(s) 30
TaaI ACNGT 2 cut(s) 360, 535
TaiI ACGT 1 cut(s) 502
TaqI TCGA 3 cut(s) 166, 286, 421
TasI AATT 7 cut(s) 196, 206, 320, 325, 367, 408, 488
TatI WGTACW 1 cut(s) 509
TauI GCSGC 1 cut(s) 221
TfiI GAWTC 1 cut(s) 17
Tru1I TTAA 4 cut(s) 66, 146, 333, 411
Tru9I TTAA 4 cut(s) 66, 146, 333, 411
TspDTI ATGAA 3 cut(s) 17, 377, 393
TspGWI ACGGA 1 cut(s) 271
TspMI CCCGGG 1 cut(s) 243
VspI ATTAAT 2 cut(s) 333, 411
XapI RAATTY 2 cut(s) 196, 325
XceI RCATGY 1 cut(s) 526
XmaI CCCGGG 1 cut(s) 243
XmnI GAANNNNTTC 1 cut(s) 384
XspI CTAG 2 cut(s) 224, 458
ZrmI AGTACT 1 cut(s) 511
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.