ATMG01170

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Key component of the proton channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
Mt
OG0014087
Physical Location & Seq
Reverse (-)
296820 .. 298204
1385 bp
Loading structure...
UTR
Exon/CDS
Intron
ATMG01170.1

Sequence Viewer

Length: 1050 bp
ATGGAGAGGTTAACCCGGTTGAATCACTTCTTAGTTAATATGCGCTGGGATTTTTACGAGGGAGTGATTCAGGCCGGATATATTAGGAACCTCCAGCGAGAATTGGATCATACTCCCGCGGAACTCCTCGGGAGCAAACTGGACCTTATTTTTTTTAGGGAATCGCTTAATTTATCTACGTACGTTAATAACTGGTATATGCAGAATTTGGGCGTTCCTGGCCCTGTCAATTTTATTGAAAAATATCACGACGCCTGTTTTTCTAACTATATGAAACTTATGGAAATCCCCTCCCCACTGGACCAATTTGAGATTGTCCCATTGATTCCTATGCATATCGGAAACTTCTATTTCTCATTCACAAATCCATCTTTGTTCATGCTGCTAACTCTGAGTTTTTTCCTACTTCTGATTCATTTTGTTACTAAAAAGGGAGGAGGAAACTTAGTCCCAAATGCTTGGCAATCCTTGGTAGAGCTTCTTTATGATTTCGTGCTGAACCTGGTAAAGGAACAAATAGGTGGTCTTTCCGGGAATGTGAAACAAATGTTTTTCCCTTGCATCTTGGTCACTTTTCTTTTTTTGTTATTTTGTAATCTTCAGGGTATGATACCTTATAGCTTCACAGTGACAAGTCATTTTCTCATTACTTTGGCTCTCTCATTTTCGATTTTTATTGGCATTACTATAGTGGGATTTCAAAGACATGGGCTTCATTTTTTCAGCTTTTTATTACCCGCAGGAGTCCCACTGCCGTTAGCACCTTTTTTAGTACTCCTTGAGCTAATTTCTTATTGTTTTCGCGCATTAAGCTTAGGAATACGTTTATTTGCTAATATGATGGCCGGTCATAGTTTAGTAAAGATTTTAAGTGGGTTCGCTTGGACTATGCTATGTATGAATGATATTTTCTATTTTATAGGGGCTCTTGGTCCTTTATTTATAGTTCTTGCATTAACCGGTCTGGAATTAGGTGTAGCTATATTACAAGCTTATGTTTTTACGATCTTAATCTGTATTTACTTGAATGATGCTATAAATCTCCATTAA

Protein Analysis

349

Amino Acids

39.74

Weight (kDa)

6.35

Isoelectric Point (pI)

34.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP-synt_A PF00119 125 - 343 2.3e-56 ATP synthase A chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 119, 804
AciI CCGC 3 cut(s) 117, 119, 738
AclWI GGATC 1 cut(s) 114
AcoI YGGCCR 1 cut(s) 843
AcsI RAATTY 1 cut(s) 205
AcuI CTGAAG 1 cut(s) 584
AcyI GRCGYC 1 cut(s) 252
AfaI GTAC 2 cut(s) 182, 774
AfiI CCNNNNNNNGG 1 cut(s) 508
AgeI ACCGGT 1 cut(s) 959
AgsI TTSAA 4 cut(s) 22, 239, 701, 1027
AjnI CCWGG 2 cut(s) 217, 501
AluBI AGCT 7 cut(s) 478, 621, 726, 784, 813, 980, 992
AluI AGCT 7 cut(s) 478, 621, 726, 784, 813, 980, 992
AlwI GGATC 1 cut(s) 114
Ama87I CYCGRG 1 cut(s) 128
AoxI GGCC 3 cut(s) 72, 220, 843
ApeKI GCWGC 1 cut(s) 382
ApoI RAATTY 1 cut(s) 205
ArsI GACNNNNNNTTYG 2 cut(s) 508, 540
AsiGI ACCGGT 1 cut(s) 959
Asp700I GAANNNNTTC 1 cut(s) 26
AspLEI GCGC 2 cut(s) 45, 806
AspS9I GGNCC 4 cut(s) 142, 221, 301, 932
AsuC2I CCSGG 2 cut(s) 16, 532
AvaI CYCGRG 1 cut(s) 128
AvaII GGWCC 3 cut(s) 142, 301, 932
BanII GRGCYC 1 cut(s) 928
BbvI GCAGC 1 cut(s) 369
BccI CCATC 2 cut(s) 376, 835
BceAI ACGGC 1 cut(s) 739
BciT130I CCWGG 2 cut(s) 219, 503
BcnI CCSGG 2 cut(s) 16, 532
BfmI CTRYAG 1 cut(s) 687
BisI GCNGC 1 cut(s) 383
BlsI GCNGC 1 cut(s) 384
BmcAI AGTACT 1 cut(s) 774
Bme1390I CCNGG 4 cut(s) 16, 219, 503, 532
Bme18I GGWCC 3 cut(s) 142, 301, 932
BmeT110I CYCGRG 1 cut(s) 128
BmgT120I GGNCC 4 cut(s) 142, 221, 301, 932
BmiI GGNNCC 1 cut(s) 89
BmrFI CCNGG 4 cut(s) 16, 219, 503, 532
BmsI GCATC 2 cut(s) 570, 1021
BpmI CTGGAG 1 cut(s) 77
Bpu10I CCTNAGC 1 cut(s) 814
BpuEI CTTGAG 1 cut(s) 800
BpuMI CCSGG 2 cut(s) 16, 532
BsaAI YACGTR 1 cut(s) 180
BsaBI GATNNNNATC 1 cut(s) 1010
BsaHI GRCGYC 1 cut(s) 252
BsaJI CCNNGG 3 cut(s) 117, 127, 468
BsaWI WCCGGW 1 cut(s) 959
Bsc4I CCNNNNNNNGG 1 cut(s) 508
Bse118I RCCGGY 2 cut(s) 845, 959
Bse1I ACTGG 3 cut(s) 144, 197, 303
Bse8I GATNNNNATC 1 cut(s) 1010
BseBI CCWGG 2 cut(s) 219, 503
BseDI CCNNGG 3 cut(s) 117, 127, 468
BseJI GATNNNNATC 1 cut(s) 1010
BseLI CCNNNNNNNGG 1 cut(s) 508
BseMII CTCAG 1 cut(s) 383
BseNI ACTGG 3 cut(s) 144, 197, 303
BseRI GAGGAG 2 cut(s) 116, 450
BseXI GCAGC 1 cut(s) 369
BseYI CCCAGC 1 cut(s) 45
Bsh1236I CGCG 2 cut(s) 119, 804
BshFI GGCC 3 cut(s) 74, 222, 845
BshTI ACCGGT 1 cut(s) 959
BsiHKCI CYCGRG 1 cut(s) 128
BsiSI CCGG 5 cut(s) 16, 75, 531, 846, 960
BsiWI CGTACG 1 cut(s) 180
BslFI GGGAC 3 cut(s) 302, 434, 731
BslI CCNNNNNNNGG 1 cut(s) 508
BsmFI GGGAC 3 cut(s) 302, 434, 731
BsnI GGCC 3 cut(s) 74, 222, 845
BsoBI CYCGRG 1 cut(s) 128
Bsp1286I GDGCHC 1 cut(s) 928
Bsp143I GATC 2 cut(s) 106, 1005
BspACI CCGC 3 cut(s) 117, 119, 738
BspANI GGCC 3 cut(s) 74, 222, 845
BspCNI CTCAG 1 cut(s) 384
BspFNI CGCG 2 cut(s) 119, 804
BspLI GGNNCC 1 cut(s) 89
BspPI GGATC 1 cut(s) 114
BsrFI RCCGGY 2 cut(s) 845, 959
BsrI ACTGG 3 cut(s) 144, 197, 303
BssAI RCCGGY 2 cut(s) 845, 959
BssECI CCNNGG 3 cut(s) 117, 127, 468
BssMI GATC 2 cut(s) 106, 1005
BssNI GRCGYC 1 cut(s) 252
BssT1I CCWWGG 1 cut(s) 468
Bst2UI CCWGG 2 cut(s) 219, 503
Bst4CI ACNGT 1 cut(s) 628
BstACI GRCGYC 1 cut(s) 252
BstBAI YACGTR 1 cut(s) 180
BstDEI CTNAG 4 cut(s) 31, 392, 445, 814
BstDSI CCRYGG 1 cut(s) 117
BstENI CCTNNNNNAGG 1 cut(s) 506
BstFNI CGCG 2 cut(s) 119, 804
BstHHI GCGC 2 cut(s) 45, 806
BstKTI GATC 2 cut(s) 109, 1008
BstMBI GATC 2 cut(s) 106, 1005
BstMWI GCNNNNNNNGC 2 cut(s) 219, 810
BstNI CCWGG 2 cut(s) 219, 503
BstSCI CCNGG 4 cut(s) 14, 217, 501, 530
BstSFI CTRYAG 1 cut(s) 687
BstSNI TACGTA 1 cut(s) 180
BstUI CGCG 2 cut(s) 119, 804
BstV1I GCAGC 1 cut(s) 369
BstXI CCANNNNNNTGG 1 cut(s) 459
BsuRI GGCC 3 cut(s) 74, 222, 845
BtgI CCRYGG 1 cut(s) 117
BtsI GCAGTG 1 cut(s) 749
BtsIMutI CAGTG 3 cut(s) 296, 633, 749
CfoI GCGC 2 cut(s) 45, 806
Cfr10I RCCGGY 2 cut(s) 845, 959
Cfr13I GGNCC 4 cut(s) 142, 221, 301, 932
Cfr42I CCGCGG 1 cut(s) 120
CseI GACGC 1 cut(s) 260
CsiI ACCWGGT 1 cut(s) 501
Csp6I GTAC 2 cut(s) 181, 773
CspAI ACCGGT 1 cut(s) 959
CviAII CATG 2 cut(s) 379, 707
CviQI GTAC 2 cut(s) 181, 773
DdeI CTNAG 4 cut(s) 31, 392, 445, 814
DpnI GATC 2 cut(s) 108, 1007
DpnII GATC 2 cut(s) 106, 1005
EaeI YGGCCR 1 cut(s) 843
Eco105I TACGTA 1 cut(s) 180
Eco130I CCWWGG 1 cut(s) 468
Eco24I GRGCYC 1 cut(s) 928
Eco47I GGWCC 3 cut(s) 142, 301, 932
Eco57I CTGAAG 1 cut(s) 584
Eco88I CYCGRG 1 cut(s) 128
EcoNI CCTNNNNNAGG 1 cut(s) 506
EcoRII CCWGG 2 cut(s) 217, 501
EcoT14I CCWWGG 1 cut(s) 468
EcoT22I ATGCAT 1 cut(s) 336
EcoT38I GRGCYC 1 cut(s) 928
ErhI CCWWGG 1 cut(s) 468
FaeI CATG 2 cut(s) 382, 710
FaqI GGGAC 3 cut(s) 302, 434, 731
FatI CATG 2 cut(s) 378, 706
FauI CCCGC 2 cut(s) 124, 745
Fnu4HI GCNGC 1 cut(s) 383
FriOI GRGCYC 1 cut(s) 928
Fsp4HI GCNGC 1 cut(s) 383
GlaI GCGC 2 cut(s) 44, 805
GluI GCNGC 1 cut(s) 383
GsaI CCCAGC 1 cut(s) 49
GsuI CTGGAG 1 cut(s) 77
HaeIII GGCC 3 cut(s) 74, 222, 845
HapII CCGG 5 cut(s) 16, 75, 531, 846, 960
HgaI GACGC 1 cut(s) 260
HhaI GCGC 2 cut(s) 45, 806
Hin1I GRCGYC 1 cut(s) 252
Hin1II CATG 2 cut(s) 382, 710
Hin6I GCGC 2 cut(s) 43, 804
HinP1I GCGC 2 cut(s) 43, 804
HincII GTYRAC 1 cut(s) 12
HindII GTYRAC 1 cut(s) 12
HindIII AAGCTT 2 cut(s) 811, 990
HinfI GANTC 6 cut(s) 22, 67, 161, 325, 412, 744
HpaI GTTAAC 1 cut(s) 12
HpaII CCGG 5 cut(s) 16, 75, 531, 846, 960
Hpy166II GTNNAC 1 cut(s) 12
Hpy188I TCNGA 3 cut(s) 341, 393, 411
Hpy188III TCNNGA 3 cut(s) 130, 248, 965
Hpy8I GTNNAC 1 cut(s) 12
Hpy99I CGWCG 1 cut(s) 254
HpyCH4III ACNGT 1 cut(s) 628
HpyCH4IV ACGT 3 cut(s) 179, 183, 823
HpyCH4V TGCA 4 cut(s) 202, 334, 561, 953
HpyF10VI GCNNNNNNNGC 2 cut(s) 219, 810
HpyF3I CTNAG 4 cut(s) 31, 392, 445, 814
HpySE526I ACGT 3 cut(s) 179, 183, 823
Hsp92I GRCGYC 1 cut(s) 252
Hsp92II CATG 2 cut(s) 382, 710
HspAI GCGC 2 cut(s) 43, 804
KspAI GTTAAC 1 cut(s) 12
KspI CCGCGG 1 cut(s) 120
Kzo9I GATC 2 cut(s) 106, 1005
LmnI GCTCC 1 cut(s) 132
Lsp1109I GCAGC 1 cut(s) 369
LweI GCATC 2 cut(s) 570, 1021
MabI ACCWGGT 1 cut(s) 501
MaeII ACGT 3 cut(s) 179, 183, 823
MaeIII GTNAC 3 cut(s) 421, 568, 628
MalI GATC 2 cut(s) 108, 1007
MboI GATC 2 cut(s) 106, 1005
MboII GAAGA 1 cut(s) 590
MhlI GDGCHC 1 cut(s) 928
MluCI AATT 7 cut(s) 101, 169, 205, 229, 305, 786, 968
MlyI GAGTC 1 cut(s) 753
MnlI CCTC 6 cut(s) 52, 101, 137, 301, 428, 431
Mph1103I ATGCAT 1 cut(s) 336
MroXI GAANNNNTTC 1 cut(s) 26
MseI TTAA 9 cut(s) 11, 36, 168, 186, 809, 869, 956, 1010, 1048
MspA1I CMGCKG 1 cut(s) 119
MspI CCGG 5 cut(s) 16, 75, 531, 846, 960
MspR9I CCNGG 4 cut(s) 16, 219, 503, 532
MvaI CCWGG 2 cut(s) 219, 503
MvnI CGCG 2 cut(s) 119, 804
MwoI GCNNNNNNNGC 2 cut(s) 219, 810
NciI CCSGG 2 cut(s) 16, 532
NdeII GATC 2 cut(s) 106, 1005
NlaIII CATG 2 cut(s) 382, 710
NlaIV GGNNCC 1 cut(s) 89
NmuCI GTSAC 2 cut(s) 568, 628
NsiI ATGCAT 1 cut(s) 336
PdmI GAANNNNTTC 1 cut(s) 26
PfeI GAWTC 5 cut(s) 22, 67, 161, 325, 412
Pfl23II CGTACG 1 cut(s) 180
PfoI TCCNGGA 1 cut(s) 530
PinAI ACCGGT 1 cut(s) 959
PkrI GCNGC 1 cut(s) 384
PleI GAGTC 1 cut(s) 752
PpsI GAGTC 1 cut(s) 752
Ppu21I YACGTR 1 cut(s) 180
Psp6I CCWGG 2 cut(s) 217, 501
PspFI CCCAGC 1 cut(s) 45
PspGI CCWGG 2 cut(s) 217, 501
PspLI CGTACG 1 cut(s) 180
PspN4I GGNNCC 1 cut(s) 89
PspPI GGNCC 4 cut(s) 142, 221, 301, 932
RsaI GTAC 2 cut(s) 182, 774
RsaNI GTAC 2 cut(s) 181, 773
SacII CCGCGG 1 cut(s) 120
SaqAI TTAA 9 cut(s) 11, 36, 168, 186, 809, 869, 956, 1010, 1048
SatI GCNGC 1 cut(s) 383
Sau3AI GATC 2 cut(s) 106, 1005
Sau96I GGNCC 4 cut(s) 142, 221, 301, 932
ScaI AGTACT 1 cut(s) 774
SchI GAGTC 1 cut(s) 753
ScrFI CCNGG 4 cut(s) 16, 219, 503, 532
SduI GDGCHC 1 cut(s) 928
SexAI ACCWGGT 1 cut(s) 501
SfaNI GCATC 2 cut(s) 570, 1021
SfcI CTRYAG 1 cut(s) 687
Sfr303I CCGCGG 1 cut(s) 120
SgrBI CCGCGG 1 cut(s) 120
SinI GGWCC 3 cut(s) 142, 301, 932
SmlI CTYRAG 1 cut(s) 779
SmoI CTYRAG 1 cut(s) 779
SnaBI TACGTA 1 cut(s) 180
Sse9I AATT 7 cut(s) 101, 169, 205, 229, 305, 786, 968
SsiI CCGC 3 cut(s) 117, 119, 738
StyD4I CCNGG 4 cut(s) 14, 217, 501, 530
StyI CCWWGG 1 cut(s) 468
TaaI ACNGT 1 cut(s) 628
TaiI ACGT 3 cut(s) 182, 186, 826
TaqI TCGA 1 cut(s) 668
TasI AATT 7 cut(s) 101, 169, 205, 229, 305, 786, 968
TatI WGTACW 1 cut(s) 772
TfiI GAWTC 5 cut(s) 22, 67, 161, 325, 412
Tru1I TTAA 9 cut(s) 11, 36, 168, 186, 809, 869, 956, 1010, 1048
Tru9I TTAA 9 cut(s) 11, 36, 168, 186, 809, 869, 956, 1010, 1048
TscAI CASTG 3 cut(s) 303, 633, 756
TseFI GTSAC 2 cut(s) 568, 628
TseI GCWGC 1 cut(s) 382
Tsp45I GTSAC 2 cut(s) 568, 628
TspDTI ATGAA 5 cut(s) 287, 367, 404, 704, 914
TspRI CASTG 3 cut(s) 303, 633, 756
VpaK11BI GGWCC 3 cut(s) 142, 301, 932
XagI CCTNNNNNAGG 1 cut(s) 506
XapI RAATTY 1 cut(s) 205
XmnI GAANNNNTTC 1 cut(s) 26
ZrmI AGTACT 1 cut(s) 774
Zsp2I ATGCAT 1 cut(s) 336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.