Rroxscaffold_2G00119300

ATP synthase A chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0014087
Physical Location & Seq
Forward (+)
51191847 .. 51192611
765 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00119300.1

Sequence Viewer

Length: 765 bp
ATGAATATAGGAAACTTGTATTTCTCATTCACAAATCCTTCTTTGTTTATGCTGCTAACTCTCAGTTTGGTCCTACTTCTGGTTCATTTTGTTACTAAAAACGGAGGAGGAAACTCAGTACCAAATGCTTGGCAATCGTCGGTAGAGTTTATTTATGATTTCGTGCTGAACCTCGTAAACGAACAAATAGGTGGCCTTTCCGGAAATGTTAAACAAAAGTTTTTCCCTCGCATCTCGGTCACTTTTACTTTTTCGTTATTTCGTAATCCCCAGGGTATGATACCTTATAGCTTCACAGTTACAAGTCATTTTCTCATTACTTTGGGTCTCTCATTTTCTATTTTTATTGGCATTACTATAGTGGGATTTCAAAGAAATGGGCTTCATTTTTTAAGCATCTCATTACCCGCAGGAGTCCCACTGCCGTTAGCACCTTTTTTAGTACTCCTTGAGCTAATTCCTCATTGTTTTCGCGCATTAAGCTCAGGAATACGTTTATTTGCTAATATGATGGCCGGTCATAGTTCAGTAAAGATTTTAAGTGGGTTCGCTTGGACTATGCTATGTATGAATGATCTTTTATTTTTTATAGGAGATCCTGGTCCTTTATTTATAGTTCTTGCATTAACCGGTCCGGAATTAGGTGTAGCTATATCACAAGCTCATGTTTCTACGATCTCAATCTGTATTTACTTGAATGATGCTACAAATCTCCATCAAAGTGGTTATTTATTTATAATTGAACAAAAGGGAGTACGAAAGTAG

Protein Analysis

254

Amino Acids

27.9

Weight (kDa)

8.86

Isoelectric Point (pI)

26.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP-synt_A PF00119 15 - 233 1.6e-42 ATP synthase A chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 737
AccII CGCG 1 cut(s) 474
AccIII TCCGGA 2 cut(s) 200, 634
AciI CCGC 1 cut(s) 408
AclWI GGATC 1 cut(s) 590
AcoI YGGCCR 1 cut(s) 513
AfaI GTAC 3 cut(s) 120, 444, 756
AfiI CCNNNNNNNGG 2 cut(s) 79, 641
AgeI ACCGGT 1 cut(s) 629
AgsI TTSAA 3 cut(s) 371, 697, 743
AjnI CCWGG 2 cut(s) 270, 598
AluBI AGCT 5 cut(s) 291, 454, 483, 650, 662
AluI AGCT 5 cut(s) 291, 454, 483, 650, 662
Alw26I GTCTC 1 cut(s) 332
AlwI GGATC 1 cut(s) 590
Aor13HI TCCGGA 2 cut(s) 200, 634
AoxI GGCC 2 cut(s) 193, 513
ApeKI GCWGC 1 cut(s) 52
AsiGI ACCGGT 1 cut(s) 629
AspLEI GCGC 1 cut(s) 476
AspS9I GGNCC 3 cut(s) 70, 602, 632
AvaII GGWCC 3 cut(s) 70, 602, 632
BbvI GCAGC 1 cut(s) 39
BccI CCATC 2 cut(s) 505, 723
BceAI ACGGC 1 cut(s) 409
BciT130I CCWGG 2 cut(s) 272, 600
BcoDI GTCTC 1 cut(s) 332
BfmI CTRYAG 1 cut(s) 357
BisI GCNGC 1 cut(s) 53
BlsI GCNGC 1 cut(s) 54
BmcAI AGTACT 1 cut(s) 444
Bme1390I CCNGG 2 cut(s) 272, 600
Bme18I GGWCC 3 cut(s) 70, 602, 632
BmgT120I GGNCC 3 cut(s) 70, 602, 632
BmrFI CCNGG 2 cut(s) 272, 600
BmsI GCATC 3 cut(s) 240, 405, 691
Bpu10I CCTNAGC 1 cut(s) 484
BpuEI CTTGAG 1 cut(s) 470
BsaBI GATNNNNATC 1 cut(s) 680
BsaI GGTCTC 1 cut(s) 332
BsaJI CCNNGG 2 cut(s) 270, 271
BsaWI WCCGGW 3 cut(s) 200, 629, 634
Bsc4I CCNNNNNNNGG 2 cut(s) 79, 641
Bse118I RCCGGY 2 cut(s) 515, 629
Bse8I GATNNNNATC 1 cut(s) 680
BseAI TCCGGA 2 cut(s) 200, 634
BseBI CCWGG 2 cut(s) 272, 600
BseDI CCNNGG 2 cut(s) 270, 271
BseJI GATNNNNATC 1 cut(s) 680
BseLI CCNNNNNNNGG 2 cut(s) 79, 641
BseMII CTCAG 3 cut(s) 76, 129, 498
BseRI GAGGAG 1 cut(s) 120
BseXI GCAGC 1 cut(s) 39
Bsh1236I CGCG 1 cut(s) 474
BshFI GGCC 2 cut(s) 195, 515
BshTI ACCGGT 1 cut(s) 629
BsiSI CCGG 4 cut(s) 201, 516, 630, 635
BslFI GGGAC 1 cut(s) 401
BslI CCNNNNNNNGG 2 cut(s) 79, 641
BsmAI GTCTC 1 cut(s) 332
BsmFI GGGAC 1 cut(s) 401
BsnI GGCC 2 cut(s) 195, 515
Bso31I GGTCTC 1 cut(s) 332
Bsp13I TCCGGA 2 cut(s) 200, 634
Bsp143I GATC 3 cut(s) 574, 595, 675
BspACI CCGC 1 cut(s) 408
BspANI GGCC 2 cut(s) 195, 515
BspCNI CTCAG 3 cut(s) 75, 128, 497
BspEI TCCGGA 2 cut(s) 200, 634
BspFNI CGCG 1 cut(s) 474
BspPI GGATC 1 cut(s) 590
BspTNI GGTCTC 1 cut(s) 332
BsrFI RCCGGY 2 cut(s) 515, 629
BssAI RCCGGY 2 cut(s) 515, 629
BssECI CCNNGG 2 cut(s) 270, 271
BssMI GATC 3 cut(s) 574, 595, 675
Bst2UI CCWGG 2 cut(s) 272, 600
Bst4CI ACNGT 1 cut(s) 298
BstDEI CTNAG 3 cut(s) 62, 115, 484
BstFNI CGCG 1 cut(s) 474
BstHHI GCGC 1 cut(s) 476
BstKTI GATC 3 cut(s) 577, 598, 678
BstMAI GTCTC 1 cut(s) 332
BstMBI GATC 3 cut(s) 574, 595, 675
BstMWI GCNNNNNNNGC 1 cut(s) 480
BstNI CCWGG 2 cut(s) 272, 600
BstSCI CCNGG 2 cut(s) 270, 598
BstSFI CTRYAG 1 cut(s) 357
BstUI CGCG 1 cut(s) 474
BstV1I GCAGC 1 cut(s) 39
BstX2I RGATCY 1 cut(s) 595
BstXI CCANNNNNNTGG 2 cut(s) 129, 722
BstYI RGATCY 1 cut(s) 595
BsuRI GGCC 2 cut(s) 195, 515
BtsI GCAGTG 1 cut(s) 419
BtsIMutI CAGTG 1 cut(s) 419
CfoI GCGC 1 cut(s) 476
Cfr10I RCCGGY 2 cut(s) 515, 629
Cfr13I GGNCC 3 cut(s) 70, 602, 632
CpoI CGGWCCG 1 cut(s) 632
Csp6I GTAC 3 cut(s) 119, 443, 755
CspAI ACCGGT 1 cut(s) 629
CspI CGGWCCG 1 cut(s) 632
CviAII CATG 1 cut(s) 665
CviJI RGCY 8 cut(s) 195, 291, 382, 454, 483, 515, 650, 662
CviKI_1 RGCY 8 cut(s) 195, 291, 382, 454, 483, 515, 650, 662
CviQI GTAC 3 cut(s) 119, 443, 755
DdeI CTNAG 3 cut(s) 62, 115, 484
DpnI GATC 3 cut(s) 576, 597, 677
DpnII GATC 3 cut(s) 574, 595, 675
EaeI YGGCCR 1 cut(s) 513
Eco31I GGTCTC 1 cut(s) 332
Eco47I GGWCC 3 cut(s) 70, 602, 632
EcoRII CCWGG 2 cut(s) 270, 598
FaeI CATG 1 cut(s) 668
FaqI GGGAC 1 cut(s) 401
FatI CATG 1 cut(s) 664
FauI CCCGC 1 cut(s) 415
Fnu4HI GCNGC 1 cut(s) 53
Fsp4HI GCNGC 1 cut(s) 53
GlaI GCGC 1 cut(s) 475
GluI GCNGC 1 cut(s) 53
HaeIII GGCC 2 cut(s) 195, 515
HapII CCGG 4 cut(s) 201, 516, 630, 635
HhaI GCGC 1 cut(s) 476
Hin1II CATG 1 cut(s) 668
Hin6I GCGC 1 cut(s) 474
HinP1I GCGC 1 cut(s) 474
HinfI GANTC 1 cut(s) 414
HpaII CCGG 4 cut(s) 201, 516, 630, 635
Hpy166II GTNNAC 1 cut(s) 178
Hpy188III TCNNGA 3 cut(s) 201, 486, 635
Hpy8I GTNNAC 1 cut(s) 178
Hpy99I CGWCG 1 cut(s) 142
HpyAV CCTTC 1 cut(s) 48
HpyCH4III ACNGT 1 cut(s) 298
HpyCH4IV ACGT 1 cut(s) 493
HpyCH4V TGCA 1 cut(s) 623
HpyF10VI GCNNNNNNNGC 1 cut(s) 480
HpyF3I CTNAG 3 cut(s) 62, 115, 484
HpySE526I ACGT 1 cut(s) 493
Hsp92II CATG 1 cut(s) 668
HspAI GCGC 1 cut(s) 474
Kpn2I TCCGGA 2 cut(s) 200, 634
Kzo9I GATC 3 cut(s) 574, 595, 675
Lsp1109I GCAGC 1 cut(s) 39
LweI GCATC 3 cut(s) 240, 405, 691
MaeII ACGT 1 cut(s) 493
MaeIII GTNAC 3 cut(s) 91, 238, 298
MalI GATC 3 cut(s) 576, 597, 677
MboI GATC 3 cut(s) 574, 595, 675
MflI RGATCY 1 cut(s) 595
MluCI AATT 3 cut(s) 456, 638, 738
MlyI GAGTC 1 cut(s) 423
MnlI CCTC 5 cut(s) 98, 101, 182, 237, 471
MroI TCCGGA 2 cut(s) 200, 634
MseI TTAA 5 cut(s) 210, 392, 479, 539, 626
MslI CAYNNNNRTG 1 cut(s) 720
MspI CCGG 4 cut(s) 201, 516, 630, 635
MspR9I CCNGG 2 cut(s) 272, 600
MvaI CCWGG 2 cut(s) 272, 600
MvnI CGCG 1 cut(s) 474
MwoI GCNNNNNNNGC 1 cut(s) 480
NdeII GATC 3 cut(s) 574, 595, 675
NlaIII CATG 1 cut(s) 668
NmuCI GTSAC 1 cut(s) 238
PasI CCCWGGG 1 cut(s) 271
PinAI ACCGGT 1 cut(s) 629
PkrI GCNGC 1 cut(s) 54
PleI GAGTC 1 cut(s) 422
PpsI GAGTC 1 cut(s) 422
PsiI TTATAA 1 cut(s) 737
Psp6I CCWGG 2 cut(s) 270, 598
PspGI CCWGG 2 cut(s) 270, 598
PspPI GGNCC 3 cut(s) 70, 602, 632
PsuI RGATCY 1 cut(s) 595
RsaI GTAC 3 cut(s) 120, 444, 756
RsaNI GTAC 3 cut(s) 119, 443, 755
RseI CAYNNNNRTG 1 cut(s) 720
Rsr2I CGGWCCG 1 cut(s) 632
RsrII CGGWCCG 1 cut(s) 632
SaqAI TTAA 5 cut(s) 210, 392, 479, 539, 626
SatI GCNGC 1 cut(s) 53
Sau3AI GATC 3 cut(s) 574, 595, 675
Sau96I GGNCC 3 cut(s) 70, 602, 632
ScaI AGTACT 1 cut(s) 444
SchI GAGTC 1 cut(s) 423
ScrFI CCNGG 2 cut(s) 272, 600
SfaNI GCATC 3 cut(s) 240, 405, 691
SfcI CTRYAG 1 cut(s) 357
SinI GGWCC 3 cut(s) 70, 602, 632
SmiMI CAYNNNNRTG 1 cut(s) 720
SmlI CTYRAG 1 cut(s) 449
SmoI CTYRAG 1 cut(s) 449
Sse9I AATT 3 cut(s) 456, 638, 738
SsiI CCGC 1 cut(s) 408
StyD4I CCNGG 2 cut(s) 270, 598
TaaI ACNGT 1 cut(s) 298
TaiI ACGT 1 cut(s) 496
TaqII GACCGA 1 cut(s) 226
TasI AATT 3 cut(s) 456, 638, 738
TatI WGTACW 1 cut(s) 442
Tru1I TTAA 5 cut(s) 210, 392, 479, 539, 626
Tru9I TTAA 5 cut(s) 210, 392, 479, 539, 626
TscAI CASTG 1 cut(s) 426
TseFI GTSAC 1 cut(s) 238
TseI GCWGC 1 cut(s) 52
Tsp45I GTSAC 1 cut(s) 238
TspDTI ATGAA 4 cut(s) 17, 74, 374, 584
TspGWI ACGGA 1 cut(s) 117
TspRI CASTG 1 cut(s) 426
VpaK11BI GGWCC 3 cut(s) 70, 602, 632
ZrmI AGTACT 1 cut(s) 444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.