FvH4_1g02950

protein ubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0013581
Physical Location & Seq
Reverse (-)
1664318 .. 1672225
7908 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g02950.t2

Sequence Viewer

Length: 3363 bp
ATGGCAAGTCTTTCGGGCGTCTCCAATTCACCTATTCTGGAAGTTCTTAACCGTAATAACTATAAAAGTTGGAGGTTGCGGATGAAGACTTATTTGTTAGTTGAAGATCTATGGGAGGCTGTCGAAGCAATCAGGGAACCTGCTGCAAAACCTGAAGATGATGAACTCGAACACAAGGCTTGGAAGAAGAAGAACGTTAAGGCTTTATACGCAATCCAAAATTCCTGCGGGCCGGAGATGTTTCATTTTATAAGCGAAACTGAAACTGCCAAAACTGCATGGGAGACTTTGGAAAAAGTTTCGATATTAAGAGGAAATAATGTTGAGAACAGTGCTGAAAACTTCAAGCCCTACATACCGTTCACCAAATTTGTGAGCGAAGGTGATTGGGATAAGGCAAAGGAGTGCCTTAAACTTAAAGGACTAGACCTCCTCAGCGCCGTAAGAGCAATAGATCCGAAAGACGAGTATGGAGACGCAGCCATTCACGTTGCAGCCATTAAAGGGCATGTGAATATCGTGAAAGACTTGATGTTGTTGATGCCCCAAGAAGATTTGAGAATGAAAAACGCTAAAGGTTGCACAGCTCTTCATTTAGCAGTATTCAATGGGCAACTGCATACTTCGAAAGAGTTGGGGCTGTTGATGGGAGGAGAAATAGACAGCGTTGGGTACACAGCCCTCCACTATGCAGCCCATTATGGGAAGGTGGGCATTTTGAAAGAGTTGGTGCTCTGTATGAGACCAAAAGACTTGAAAATAAAAGACAACAATGGTTACACAGCTCTTCACCTAGCAGTCAAGAAGGGAAATGTGGCTATGATGAAATATTTAGCGGCGTTGATTGGAGAAGTGAGAGGCCCTGATGGTGACACTGCTCTTCACCTAGCAGTCAAGTTGGGGGCTGCAGAAGTTGTGAAAGAGTTGGTGTTATTGATGAGAGGGGAAGATTTGGCAATAAAAAATGATGAAGGTTATACAGCTCTTCACTTGGCAGTAAAGAAGGGTAACGTGAAAGAGTTCATGACAAAAGAAGAAGAGCGTGACTTTGATAGCCATATTCAGTTCACCTCTTATGTAACAGAAGGTGAATGGGATAATGCAAAGGAGTGCCTTACAAAACTAGATGATCCCTGTGATGCAATAACTATAGTGGATCCAAGGGACGGATATGGAGACACTGCTCTTCATGTAGCAACTAGACAAGGGCATGTGCATATTGTGAAAGAGTTGATATCATTGCTGAAAGTGAGACAAGAAATTCAGGAATTGCAAACTATAGAAGATATCATAGCTTTTGGTTCTGGTATAAATTTAGGGGTCACTGAAGAAGTTGTCAGGAAAAAGGCCAAGTACATGGTCGAGCAGTACGAGAAGTTGCTTACCATTACTTTGGAAGTACAAAATACACAAGGTTCCACAGCTGTTCACATAGCAGCATCTAATGGTAATTTAGATATTTTTAAAGAGTTGATGCCATTAATGAGAAAAGAAGGTTTAGAGATACAGGATGCTGCAGGATTCACAGCTCTTCTCAATGCAGTGGAGAAGGGTAATATCGAAATTGTGAAAGAGTTGTTGCCAGTGATGAGACAAGAAGGTCTGGAACTAAAAACAACCAAACATGGCTACAATGCACTTCACCTTGCGATCATGAATGGGCATATGAATATTGCGAAAGAGGTAATGCCATTTATGAGAAAAGAAGCTTTGGAAGAAAAAGACGGTGCAGGTTATACAGCTCTAGGTTGTGCGTTAGATGTACGCAGGGATGGAGACGTGATGGAAATAGCCAAGTATATGGCTGAAAACAACACCAAAGTATTTGGCGTACGCGTGCCTCCTTCTAGTTGGATTCCATTACTCAGGGCCCTCGATAATAGCAGATGGGATCTGTGTCGTTGTCTTTATCCTCTTACTCCGTTGGAATATTTAATGCCTGAGCACGGGAAGGCTGGTGCGCAGTTGATTGTCGATTGCTTTAGAGCGAAGCAGCTTGATATGGTCTTGGACTTACTTCGGCGTTGCTCTGGATTAGCCATCGTCATCAGTCCAACTGGTGATGCATGGCCCCCCATAATGGAATTGGCTCGCATGAGCTCTGCATTCTTAAGTGGAACTCAACTTGGCTTTTGGCAACGTTTGATCTATAATTGGTTGCGCATAGAACCTGCTAATTTAAGCACTCTTCATACTTGCATCAATATTCCACAACAAGAGGATAATCGAGACAATCAAATTGATCTCATTCGCTCAGTGACAACTTTATACAAGAAACTCGTATACCGTCTCTTTACACTTCTAGGAGTCATTCACCTACATGAAATGAAATGGATCCATACTCGAAGCATTGACATTTTACGTTGCATTAGTGAAATGCTAAAAAAGGAACCTCCTACATCATCACAACTTAACATCGTTAGAAAATCGATGTTTAAAGCTGTAGAACACGGGAATGTTGAGTTTGTTACTCATATATGTGAAGCACATCCATCACTGGCAAATTCACGTGAAGAAAGCAAAAATATATTTCAGTTTGCGGTTGAATGTCGTCAACATAAAATTTTCAGCCTTATATACGGAATCAGTGAAGAGGATCAGAGATTAAATGGACTACGTGGGGTAAATTATCCAGAGAATATATTACATTTGGCAGGGAAATTATCTCCACTCTCAAGGTTCAATCGTATTCGCGGTGCAGCTTTGCAAATGCAACGAGAACTACATTGGTTCAAGGCGGTAGAGGATATTGTACCTCTGCAGATGCGTGAAATTCCAAATTCAGATGGTATGACACCACATGAACTGTTTACCAAGAATCATGAGGCACTAAAAAAGGAGGCAGAAGATTCGATGAAAGGAACAGCAACTTCTTGTACTGTTGTCGGCGCTCTTATAGTTACCATGATGTTTGCTGCAGCATTTACAGTTCCTGGTGGAAATGATGGCAGTACAGGTGTCCCAATGTTTCTAGCTAAGAAGTTGTTTATGGTGTTTATAATTTCAGATACTCTATCACTCGTTTCTTCCACAACTTCAGTCATATTATTTTTAGGAATCCTCACGTCACGTTATGCAGAAGATGATTTTCTCAAGCATTTGCCGACAAAGATGATGTTAGGTCTTTCCACCCTTTTCATCTCTATTGCCACCATGATGATTGCCTTTTCTTCTGCCCTCATCATTATGCTCTATAAAGAATACTCATGGGCTGTTATTCCTAGCATTTTTGCTGCTAGTATTCCAGTTGCCTCATTTATATGGTTGCAATTTCCCCTCCTTATTGAGATAATATGGTCAACTTATGGACCAGGAATATTTGACAAGAAACTGAAGAAAGGCAAACCATGGATTAAACTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1121

Amino Acids

125.7

Weight (kDa)

6.58

Isoelectric Point (pI)

34.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4219 PF13961 16 - 42 1.8e-09 Domain of unknown function (DUF4219)
Retrotran_gag_2 PF14223 28 - 99 1.3e-10 gag-polypeptide of LTR copia-type
Ank_2 PF12796 151 - 210 1e-06 Ankyrin repeats (3 copies)
Ank_2 PF12796 217 - 279 1.9e-10 Ankyrin repeats (3 copies)
Ank_5 PF13857 224 - 266 2.9e-07 Ankyrin repeats (many copies)
Ank_4 PF13637 228 - 278 6.2e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 288 - 338 1.1e-07 Ankyrin repeats (3 copies)
Ank_5 PF13857 289 - 332 4.1e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 464 - 527 7.2e-10 Ankyrin repeats (3 copies)
PGG PF13962 951 - 1062 3.7e-24 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 251, 2999
AasI GACNNNNNNGTC 1 cut(s) 1599
Acc16I TGCGCA 2 cut(s) 1962, 2162
Acc36I ACCTGC 3 cut(s) 148, 1721, 2179
AccI GTMKAC 1 cut(s) 2283
AccII CGCG 2 cut(s) 1836, 2694
AciI CCGC 6 cut(s) 79, 228, 836, 2540, 2694, 2738
AclI AACGTT 2 cut(s) 195, 2140
AclWI GGATC 8 cut(s) 449, 1124, 1151, 1164, 1899, 2329, 2342, 2604
AcsI RAATTY 8 cut(s) 220, 368, 1260, 1312, 2503, 2562, 2772, 2779
AcuI CTGAAG 4 cut(s) 174, 1347, 3021, 3353
AcvI CACGTG 1 cut(s) 2510
AcyI GRCGYC 1 cut(s) 18
AfaI GTAC 9 cut(s) 674, 1355, 1370, 1401, 1764, 1833, 2754, 2878, 2953
AfiI CCNNNNNNNGG 5 cut(s) 504, 702, 1166, 1946, 2080
AflII CTTAAG 1 cut(s) 2110
AflIII ACRYGT 1 cut(s) 1834
AgsI TTSAA 8 cut(s) 104, 346, 607, 721, 757, 2546, 2683, 2734
AjiI CACGTC 2 cut(s) 1780, 3066
AjnI CCWGG 2 cut(s) 2932, 3310
Alw21I GWGCWC 3 cut(s) 735, 1947, 2102
AlwI GGATC 8 cut(s) 449, 1124, 1151, 1164, 1899, 2329, 2342, 2604
AlwNI CAGNNNCTG 1 cut(s) 2933
AoxI GGCC 5 cut(s) 230, 859, 1347, 1869, 2069
ApaI GGGCCC 1 cut(s) 1873
ApoI RAATTY 8 cut(s) 220, 368, 1260, 1312, 2503, 2562, 2772, 2779
AseI ATTAAT 1 cut(s) 1481
Asp700I GAANNNNTTC 1 cut(s) 1019
AspLEI GCGC 4 cut(s) 440, 1963, 2163, 2891
AspS9I GGNCC 6 cut(s) 230, 860, 1869, 1870, 2070, 3308
AsuII TTCGAA 1 cut(s) 626
AvaII GGWCC 1 cut(s) 3308
BaeGI GKGCMC 1 cut(s) 1873
BamHI GGATCC 2 cut(s) 1156, 2334
BanII GRGCYC 2 cut(s) 1873, 2102
BbrPI CACGTG 1 cut(s) 2510
BbsI GAAGAC 1 cut(s) 92
Bbv12I GWGCWC 3 cut(s) 735, 1947, 2102
BbvCI CCTCAGC 1 cut(s) 434
BccI CCATC 9 cut(s) 640, 860, 1766, 1777, 1881, 2048, 2500, 2780, 2939
BceAI ACGGC 1 cut(s) 425
BcgI CGANNNNNNTGC 3 cut(s) 28, 2832, 2866
BciT130I CCWGG 2 cut(s) 2934, 3312
BfmI CTRYAG 7 cut(s) 906, 1149, 1278, 1515, 2442, 2759, 2916
BfoI RGCGCY 2 cut(s) 441, 2892
BfrI CTTAAG 1 cut(s) 2110
BfuAI ACCTGC 3 cut(s) 148, 1721, 2179
BglII AGATCT 1 cut(s) 106
Bme1390I CCNGG 2 cut(s) 2934, 3312
Bme18I GGWCC 1 cut(s) 3308
BmgBI CACGTC 2 cut(s) 1780, 3066
BmgT120I GGNCC 6 cut(s) 230, 860, 1869, 1870, 2070, 3308
BmiI GGNNCC 7 cut(s) 138, 1158, 1417, 1871, 2072, 2336, 2391
BmrFI CCNGG 2 cut(s) 2934, 3312
BmsI GCATC 8 cut(s) 531, 1129, 1448, 1464, 1501, 2053, 2208, 2754
BpiI GAAGAC 1 cut(s) 92
Bpu10I CCTNAGC 2 cut(s) 434, 1941
Bpu14I TTCGAA 1 cut(s) 626
BpuEI CTTGAG 2 cut(s) 2659, 3077
Bsa29I ATCGAT 1 cut(s) 2429
BsaAI YACGTR 2 cut(s) 2510, 2618
BsaHI GRCGYC 1 cut(s) 18
BsaI GGTCTC 1 cut(s) 736
BsaJI CCNNGG 2 cut(s) 1160, 3347
BsaXI ACNNNNNCTCC 2 cut(s) 414, 444
Bsc4I CCNNNNNNNGG 5 cut(s) 504, 702, 1166, 1946, 2080
Bse1I ACTGG 4 cut(s) 1583, 2062, 2502, 3245
Bse3DI GCAATG 1 cut(s) 1238
BseBI CCWGG 2 cut(s) 2934, 3312
BseCI ATCGAT 1 cut(s) 2429
BseDI CCNNGG 2 cut(s) 1160, 3347
BseGI GGATG 4 cut(s) 87, 1516, 1777, 2488
BseLI CCNNNNNNNGG 5 cut(s) 504, 702, 1166, 1946, 2080
BseMI GCAATG 1 cut(s) 1238
BseMII CTCAG 4 cut(s) 448, 1879, 1932, 2268
BseNI ACTGG 4 cut(s) 1583, 2062, 2502, 3245
BseRI GAGGAG 2 cut(s) 422, 666
BseSI GKGCMC 1 cut(s) 1873
BsgI GTGCAG 2 cut(s) 1749, 2718
Bsh1236I CGCG 2 cut(s) 1836, 2694
BshFI GGCC 5 cut(s) 232, 861, 1349, 1871, 2071
BshVI ATCGAT 1 cut(s) 2429
BsiHKAI GWGCWC 3 cut(s) 735, 1947, 2102
BsiSI CCGG 1 cut(s) 233
BsiWI CGTACG 1 cut(s) 1831
BslFI GGGAC 2 cut(s) 1178, 2945
BslI CCNNNNNNNGG 5 cut(s) 504, 702, 1166, 1946, 2080
BsmBI CGTCTC 4 cut(s) 25, 468, 1770, 2294
BsmFI GGGAC 2 cut(s) 1178, 2945
BsmI GAATGC 1 cut(s) 2105
BsnI GGCC 5 cut(s) 232, 861, 1349, 1871, 2071
Bso31I GGTCTC 1 cut(s) 736
Bsp119I TTCGAA 1 cut(s) 626
Bsp120I GGGCCC 1 cut(s) 1869
Bsp1286I GDGCHC 4 cut(s) 735, 1873, 1947, 2102
Bsp19I CCATGG 1 cut(s) 3347
BspACI CCGC 6 cut(s) 79, 228, 836, 2540, 2694, 2738
BspANI GGCC 5 cut(s) 232, 861, 1349, 1871, 2071
BspCNI CTCAG 4 cut(s) 447, 1878, 1933, 2267
BspDI ATCGAT 1 cut(s) 2429
BspFNI CGCG 2 cut(s) 1836, 2694
BspHI TCATGA 3 cut(s) 1023, 1653, 2821
BspLI GGNNCC 7 cut(s) 138, 1158, 1417, 1871, 2072, 2336, 2391
BspMAI CTGCAG 4 cut(s) 910, 1519, 2763, 2920
BspMI ACCTGC 3 cut(s) 148, 1721, 2179
BspPI GGATC 8 cut(s) 449, 1124, 1151, 1164, 1899, 2329, 2342, 2604
BspQI GCTCTTC 7 cut(s) 594, 792, 885, 990, 1032, 1191, 1536
BspT104I TTCGAA 1 cut(s) 626
BspTI CTTAAG 1 cut(s) 2110
BspTNI GGTCTC 1 cut(s) 736
BsrDI GCAATG 1 cut(s) 1238
BsrI ACTGG 4 cut(s) 1583, 2062, 2502, 3245
BssECI CCNNGG 2 cut(s) 1160, 3347
BssNAI GTATAC 1 cut(s) 2284
BssNI GRCGYC 1 cut(s) 18
BssT1I CCWWGG 2 cut(s) 1160, 3347
Bst1107I GTATAC 1 cut(s) 2284
Bst2UI CCWGG 2 cut(s) 2934, 3312
Bst4CI ACNGT 8 cut(s) 53, 332, 360, 1727, 2288, 2808, 2881, 2929
Bst6I CTCTTC 9 cut(s) 594, 792, 885, 990, 1032, 1191, 1536, 2193, 2586
BstACI GRCGYC 1 cut(s) 18
BstAFI CTTAAG 1 cut(s) 2110
BstBAI YACGTR 2 cut(s) 2510, 2618
BstBI TTCGAA 1 cut(s) 626
BstC8I GCNNGC 3 cut(s) 230, 1838, 2092
BstDEI CTNAG 5 cut(s) 434, 1865, 1941, 2254, 2976
BstDSI CCRYGG 1 cut(s) 3347
BstF5I GGATG 4 cut(s) 87, 1516, 1777, 2488
BstFNI CGCG 2 cut(s) 1836, 2694
BstH2I RGCGCY 2 cut(s) 441, 2892
BstHHI GCGC 4 cut(s) 440, 1963, 2163, 2891
BstMWI GCNNNNNNNGC 4 cut(s) 125, 209, 275, 446
BstNI CCWGG 2 cut(s) 2934, 3312
BstNSI RCATGY 2 cut(s) 512, 1214
BstSCI CCNGG 2 cut(s) 2932, 3310
BstSFI CTRYAG 7 cut(s) 906, 1149, 1278, 1515, 2442, 2759, 2916
BstSLI GKGCMC 1 cut(s) 1873
BstUI CGCG 2 cut(s) 1836, 2694
BstV2I GAAGAC 1 cut(s) 92
BstX2I RGATCY 5 cut(s) 106, 454, 1156, 1891, 2334
BstXI CCANNNNNNTGG 3 cut(s) 1357, 1393, 1801
BstYI RGATCY 5 cut(s) 106, 454, 1156, 1891, 2334
BstZ17I GTATAC 1 cut(s) 2284
Bsu15I ATCGAT 1 cut(s) 2429
BsuRI GGCC 5 cut(s) 232, 861, 1349, 1871, 2071
BsuTUI ATCGAT 1 cut(s) 2429
BtgI CCRYGG 1 cut(s) 3347
BtrI CACGTC 2 cut(s) 1780, 3066
BtsCI GGATG 4 cut(s) 87, 1516, 1777, 2488
BtsI GCAGTG 3 cut(s) 873, 1179, 1548
BtsIMutI CAGTG 9 cut(s) 337, 873, 1179, 1323, 1548, 1590, 2262, 2495, 2593
BveI ACCTGC 3 cut(s) 148, 1721, 2179
Cac8I GCNNGC 3 cut(s) 230, 1838, 2092
CaiI CAGNNNCTG 1 cut(s) 2933
CciI TCATGA 3 cut(s) 1023, 1653, 2821
CfoI GCGC 4 cut(s) 440, 1963, 2163, 2891
Cfr13I GGNCC 6 cut(s) 230, 860, 1869, 1870, 2070, 3308
ClaI ATCGAT 1 cut(s) 2429
CseI GACGC 2 cut(s) 7, 485
Csp6I GTAC 9 cut(s) 673, 1354, 1369, 1400, 1763, 1832, 2753, 2877, 2952
CviQI GTAC 9 cut(s) 673, 1354, 1369, 1400, 1763, 1832, 2753, 2877, 2952
DdeI CTNAG 5 cut(s) 434, 1865, 1941, 2254, 2976
DraI TTTAAA 2 cut(s) 1465, 2437
DrdI GACNNNNNNGTC 1 cut(s) 1599
DseDI GACNNNNNNGTC 1 cut(s) 1599
Eam1104I CTCTTC 9 cut(s) 594, 792, 885, 990, 1032, 1191, 1536, 2193, 2586
EarI CTCTTC 9 cut(s) 594, 792, 885, 990, 1032, 1191, 1536, 2193, 2586
Ecl136II GAGCTC 1 cut(s) 2100
Eco130I CCWWGG 2 cut(s) 1160, 3347
Eco24I GRGCYC 2 cut(s) 1873, 2102
Eco31I GGTCTC 1 cut(s) 736
Eco32I GATATC 2 cut(s) 1236, 1288
Eco47I GGWCC 1 cut(s) 3308
Eco53kI GAGCTC 1 cut(s) 2100
Eco57I CTGAAG 4 cut(s) 174, 1347, 3021, 3353
Eco72I CACGTG 1 cut(s) 2510
EcoICRI GAGCTC 1 cut(s) 2100
EcoO109I RGGNCCY 3 cut(s) 860, 1869, 1870
EcoRII CCWGG 2 cut(s) 2932, 3310
EcoRV GATATC 2 cut(s) 1236, 1288
EcoT14I CCWWGG 2 cut(s) 1160, 3347
EcoT22I ATGCAT 1 cut(s) 2068
EcoT38I GRGCYC 2 cut(s) 1873, 2102
ErhI CCWWGG 2 cut(s) 1160, 3347
Esp3I CGTCTC 4 cut(s) 25, 468, 1770, 2294
FaqI GGGAC 2 cut(s) 1178, 2945
FauI CCCGC 1 cut(s) 221
FauNDI CATATG 1 cut(s) 1665
FblI GTMKAC 1 cut(s) 2283
FokI GGATG 4 cut(s) 94, 1523, 1784, 2475
FriOI GRGCYC 2 cut(s) 1873, 2102
FspI TGCGCA 2 cut(s) 1962, 2162
GlaI GCGC 4 cut(s) 439, 1962, 2162, 2890
HaeII RGCGCY 2 cut(s) 441, 2892
HaeIII GGCC 5 cut(s) 232, 861, 1349, 1871, 2071
HapII CCGG 1 cut(s) 233
HgaI GACGC 2 cut(s) 7, 485
HhaI GCGC 4 cut(s) 440, 1963, 2163, 2891
Hin1I GRCGYC 1 cut(s) 18
Hin6I GCGC 4 cut(s) 438, 1961, 2161, 2889
HinP1I GCGC 4 cut(s) 438, 1961, 2161, 2889
HincII GTYRAC 2 cut(s) 2555, 3300
HindII GTYRAC 2 cut(s) 2555, 3300
HindIII AAGCTT 1 cut(s) 1707
HinfI GANTC 7 cut(s) 1521, 1855, 2307, 2583, 2818, 2849, 3057
HpaII CCGG 1 cut(s) 233
Hpy166II GTNNAC 8 cut(s) 363, 675, 1068, 1429, 2284, 2555, 2811, 3300
Hpy188I TCNGA 5 cut(s) 459, 2601, 2785, 3007, 3362
Hpy8I GTNNAC 8 cut(s) 363, 675, 1068, 1429, 2284, 2555, 2811, 3300
HpyCH4III ACNGT 8 cut(s) 53, 332, 360, 1727, 2288, 2808, 2881, 2929
HpyF10VI GCNNNNNNNGC 4 cut(s) 125, 209, 275, 446
HpyF3I CTNAG 5 cut(s) 434, 1865, 1941, 2254, 2976
Hsp92I GRCGYC 1 cut(s) 18
HspAI GCGC 4 cut(s) 438, 1961, 2161, 2889
LguI GCTCTTC 7 cut(s) 594, 792, 885, 990, 1032, 1191, 1536
LweI GCATC 8 cut(s) 531, 1129, 1448, 1464, 1501, 2053, 2208, 2754
MflI RGATCY 5 cut(s) 106, 454, 1156, 1891, 2334
MhlI GDGCHC 4 cut(s) 735, 1873, 1947, 2102
MluI ACGCGT 1 cut(s) 1834
MlyI GAGTC 1 cut(s) 2316
MmeI TCCRAC 4 cut(s) 50, 1832, 1905, 2078
Mph1103I ATGCAT 1 cut(s) 2068
MroXI GAANNNNTTC 1 cut(s) 1019
MslI CAYNNNNRTG 6 cut(s) 2319, 2454, 2478, 3155, 3185, 3259
MspA1I CMGCKG 1 cut(s) 1424
MspCI CTTAAG 1 cut(s) 2110
MspI CCGG 1 cut(s) 233
MspR9I CCNGG 2 cut(s) 2934, 3312
Mva1269I GAATGC 1 cut(s) 2105
MvaI CCWGG 2 cut(s) 2934, 3312
MvnI CGCG 2 cut(s) 1836, 2694
MwoI GCNNNNNNNGC 4 cut(s) 125, 209, 275, 446
NcoI CCATGG 1 cut(s) 3347
NdeI CATATG 1 cut(s) 1665
NlaIV GGNNCC 7 cut(s) 138, 1158, 1417, 1871, 2072, 2336, 2391
NmuCI GTSAC 5 cut(s) 869, 1043, 1321, 2257, 3066
NsbI TGCGCA 2 cut(s) 1962, 2162
NsiI ATGCAT 1 cut(s) 2068
NspI RCATGY 2 cut(s) 512, 1214
NspV TTCGAA 1 cut(s) 626
PagI TCATGA 3 cut(s) 1023, 1653, 2821
PciSI GCTCTTC 7 cut(s) 594, 792, 885, 990, 1032, 1191, 1536
PcsI WCGNNNNNNNCGW 1 cut(s) 1368
PctI GAATGC 1 cut(s) 2105
PdmI GAANNNNTTC 1 cut(s) 1019
PfeI GAWTC 6 cut(s) 1521, 1855, 2583, 2818, 2849, 3057
Pfl23II CGTACG 1 cut(s) 1831
PleI GAGTC 1 cut(s) 2315
PmaCI CACGTG 1 cut(s) 2510
PmlI CACGTG 1 cut(s) 2510
PpsI GAGTC 1 cut(s) 2315
Ppu21I YACGTR 2 cut(s) 2510, 2618
PshBI ATTAAT 1 cut(s) 1481
PsiI TTATAA 2 cut(s) 251, 2999
Psp124BI GAGCTC 1 cut(s) 2102
Psp1406I AACGTT 2 cut(s) 195, 2140
Psp6I CCWGG 2 cut(s) 2932, 3310
PspCI CACGTG 1 cut(s) 2510
PspGI CCWGG 2 cut(s) 2932, 3310
PspLI CGTACG 1 cut(s) 1831
PspN4I GGNNCC 7 cut(s) 138, 1158, 1417, 1871, 2072, 2336, 2391
PspOMI GGGCCC 1 cut(s) 1869
PspPI GGNCC 6 cut(s) 230, 860, 1869, 1870, 2070, 3308
PstI CTGCAG 4 cut(s) 910, 1519, 2763, 2920
PstNI CAGNNNCTG 1 cut(s) 2933
PsuI RGATCY 5 cut(s) 106, 454, 1156, 1891, 2334
PvuII CAGCTG 1 cut(s) 1424
RsaI GTAC 9 cut(s) 674, 1355, 1370, 1401, 1764, 1833, 2754, 2878, 2953
RsaNI GTAC 9 cut(s) 673, 1354, 1369, 1400, 1763, 1832, 2753, 2877, 2952
RseI CAYNNNNRTG 6 cut(s) 2319, 2454, 2478, 3155, 3185, 3259
SacI GAGCTC 1 cut(s) 2102
SapI GCTCTTC 7 cut(s) 594, 792, 885, 990, 1032, 1191, 1536
Sau96I GGNCC 6 cut(s) 230, 860, 1869, 1870, 2070, 3308
SchI GAGTC 1 cut(s) 2316
ScrFI CCNGG 2 cut(s) 2934, 3312
SduI GDGCHC 4 cut(s) 735, 1873, 1947, 2102
SfaNI GCATC 8 cut(s) 531, 1129, 1448, 1464, 1501, 2053, 2208, 2754
SfcI CTRYAG 7 cut(s) 906, 1149, 1278, 1515, 2442, 2759, 2916
SfuI TTCGAA 1 cut(s) 626
SinI GGWCC 1 cut(s) 3308
SmiMI CAYNNNNRTG 6 cut(s) 2319, 2454, 2478, 3155, 3185, 3259
SmlI CTYRAG 3 cut(s) 2110, 2674, 3092
SmoI CTYRAG 3 cut(s) 2110, 2674, 3092
SsiI CCGC 6 cut(s) 79, 228, 836, 2540, 2694, 2738
SspI AATATT 5 cut(s) 830, 1672, 1931, 2206, 3318
SstI GAGCTC 1 cut(s) 2102
StyD4I CCNGG 2 cut(s) 2932, 3310
StyI CCWWGG 2 cut(s) 1160, 3347
TaaI ACNGT 8 cut(s) 53, 332, 360, 1727, 2288, 2808, 2881, 2929
TatI WGTACW 4 cut(s) 1353, 1399, 2876, 2951
TauI GCSGC 1 cut(s) 839
TfiI GAWTC 6 cut(s) 1521, 1855, 2583, 2818, 2849, 3057
TscAI CASTG 9 cut(s) 337, 880, 1186, 1330, 1548, 1590, 2262, 2502, 2593
TseFI GTSAC 5 cut(s) 869, 1043, 1321, 2257, 3066
Tsp45I GTSAC 5 cut(s) 869, 1043, 1321, 2257, 3066
TspGWI ACGGA 3 cut(s) 1182, 1911, 2595
TspRI CASTG 9 cut(s) 337, 880, 1186, 1330, 1548, 1590, 2262, 2502, 2593
Vha464I CTTAAG 1 cut(s) 2110
VpaK11BI GGWCC 1 cut(s) 3308
VspI ATTAAT 1 cut(s) 1481
XapI RAATTY 8 cut(s) 220, 368, 1260, 1312, 2503, 2562, 2772, 2779
XceI RCATGY 2 cut(s) 512, 1214
XcmI CCANNNNNNNNNTGG 1 cut(s) 2083
XmiI GTMKAC 1 cut(s) 2283
XmnI GAANNNNTTC 1 cut(s) 1019
Zsp2I ATGCAT 1 cut(s) 2068
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.