Rroxscaffold_3G00222640

protein ubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0013581
Physical Location & Seq
Forward (+)
5648114 .. 5691913
43800 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00222640.1

Sequence Viewer

Length: 2772 bp
ATGGCAAGTACTGCAGGTGTCTCCAGTTCACTTATTCTGGAAGTTCTCAACCGCAATAACTATAAAAAGTGGAGATTGCGGGTAAAAACTTATTTGTTGGCTGAGGATCTATGGGAGGTTGTCGAAGCAATAACAGAACCTGCAAATCTAGAAGATGACGAAGCAGAATATAAGTCTTGGAGAAAAAAGAATGCCAAGGCTTTATACGTTATTCAAAATTCATGCGGGCCGGAGATGTTTAATTTTATAAGCGAAACTGAAACGGCCAAAAGTGCCTGGGAGGCTTTAGAAAAAGTTTCAAGGTTAACAGGGCATGTTGAGAACAGTGCCGAAAAGTTCAAGCCCTACATACCATTCACCGAATTTGTGAGCGAAGGTGATTGGGATAAGGCAAAGGGGTGCCTTAAACTCAAAGAACTAGATCTCCACAGCGCCGTAAGAGCAATAGATCCAAGAGACGGGTTTGGAGATACAGCCATTCACGTAGCAGCCAGGAAAGGGCACGTGAATATTGTGAAAGAGTTGGTGCTTTTGTTGACCCAAGAAGATCTGAAAATGAAAAACACTAAAGGTAATACTGCTCTTCATGTAGCAGTATCTAATGGGCAGCTGCGTACTGTGAAAGAGTTGGTGCTGTTGATGGGAGAAGAAATAGACAGCGATGGTGACACAGCTCTTCACTTAGCAGTCCATTGGGAGAAGGTGGACATTGTGGAAGAGTTGGCGCTGTTTATGAGACCAATAGACTTGAAAATAAAAAACAACAATGGTTACACAGCTCTTCACCTAGCAGTCAAGAAGGGAAATGTGCCTATAGTGAAAGATTTAGCAGCGTTGGTGGGAGAAGTGAGAGGCCCTGATGGTGACACTGCTCTTCATATAGCAGTCAAGATGGACGCTACACAAATTGTGAAAGAGTTGGTGTCGTTGATGAGAAGGGAAGATTTGGCAATAAAAAATGATGAAGGCTACACAGCTTTTCATCTAGCAGTAAAGAAGGGTAATGTGCCTACTGTGAAAGAGTTCATGACAAAAGAAAAAGAGCACAATTTTGATAGCTATATTCAATTCACCACTTATGTGGAAAATGGTGATTGGGATAATGCAAAGGAGTGCCTTACAAAACTAGATGATCCCTGTGACGCAATAACGATAGTGGATCCAAGGGATGGATATGGAGACACTGCTCTTCATGTAGCAGCTAGAAAAGGGCATGTGCATATTGTGAAAGAGTTGATATCGTTGCTGAAAGTGAGACAAGAAAATTTGGAACTGAAAACCATAGAAGATTTGACAGCTTTTGATTCTGGTATAAATTTTGGAGTCAATGAACAGGTTCTCATGGAAAAGGCCCAATACATGGTTGGACAGTATGATAAAATGCTTACCAATATTTTGGATGTACAAAATGCAAAAGGTTTCACAGCCCTTCATCTAGCAGTATTTAAGGGGAATGTAAATATTGTGAAAGAGTTGGTGTCATTCATGAGAAAAGAAGGTATGGAAATAAAAGACGTCGATGGAGACACAGCTCTTAACATCGCAGTCTTAAAGGGGCATGGGAATGTGGATATTGCGAAGGAGTTGGTGCAATCGATGAGACAAAAAGGTCTGGAAGTAAAAAATAACGACGGTTTCAATGCTCTTCACCGGGCACTCCTGAATGGGCATATGCAGATCGTAAGAGAGTTGATGCCATTGATGAGACCTGAGGCTCTGGAAGAAATAAACGGTGAAGGTTATACAGCTTTGGGTTATGCGTTAGCTGTATGCAAGGATGAAGACGTGATGGAAATAGCTAAGTACATGGCTGAAAACAACAATAAAGTATTTGGCATCTTCACCCCTCCCTTGAATGGGATCCCAGTTCTCACAGTTCTCAATAATCGCAACTGGGATTTGTGTCGCGATCTCTATCCACTTACTCCGCTTGAATATCTAATGCCCGAGCACGGGAAGGATGGCGCGCACTTGATTAGAGATTGCTTTAGAGCGAAGAATCTTGATATGGCTTTGGATTTACTTCGGCGTTGCCCTAGATTGGCCATCAGTCCAACCGGCTTTGGATGGTCCCCCATAATGGAATTGGCTGGCATGCAGTCTGCATTCTTAAGTGGAACGCAACTAGGATTTTGGCAATCTTTGATCTATAATCGTTTACGCATTGAACCTGCCAATTTGAACACTCCTGAGACTCGCATCAATGTTTCAAAACAAGAAGATGATCGAGACAATCAAATGGATCTTATTCGCTCAGTTATAACTTTATATAGGAAACTTGCATACAGTCTCCTTAAACTTTTGGGAATCAATCGGTTACGTGAAGTGAAATGGATCCATACTCGAACCATTGATGTTTTACGTTGCATTAGTGAAATGCTAAAGAATGACAATCCTACATCAGCACAACTTGACATCATAAGAACATCAATCTTCAAAGCTGTTGAACACGGGCATGTTGAGTTTGTTACTCATATATGTGAAGCATATCCTCCATTCGCAAAATCAGATGAAGAAAACAAAAACATATTTCAGTTTGCGGTTGAATGTCGTCAACATAAAATTTACAGCCTTATCCATAGACTTGATGGAGAGGACCAGAGATTCTTTGCAGTGCGTGCAACCGTGTTTCCAGAGAATATGTTACATTTGGCAGCGAAATTATCTCCACTCTCAAGGTTCAATCATATTCGTGGTGCAGCATTGCAAATGCAACGAGAGCTACAATGGTTTAAGGAGGTGAAGGATATTGTACCTCGGGGAGATGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

923

Amino Acids

104.43

Weight (kDa)

6.05

Isoelectric Point (pI)

28.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4219 PF13961 16 - 42 2.7e-09 Domain of unknown function (DUF4219)
Retrotran_gag_2 PF14223 29 - 98 1.7e-10 gag-polypeptide of LTR copia-type
Ank_2 PF12796 150 - 211 1.2e-11 Ankyrin repeats (3 copies)
Ank_2 PF12796 207 - 277 2.8e-10 Ankyrin repeats (3 copies)
Ank_2 PF12796 286 - 348 1.9e-08 Ankyrin repeats (3 copies)
Ank_5 PF13857 286 - 330 7.6e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 466 - 532 2.3e-10 Ankyrin repeats (3 copies)
Ank_2 PF12796 521 - 605 1.1e-06 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 248, 2261
AarI CACCTGC 1 cut(s) 5
AasI GACNNNNNNGTC 1 cut(s) 1608
AatII GACGTC 1 cut(s) 1518
Acc36I ACCTGC 3 cut(s) 5, 148, 2179
AccB1I GGYRCC 1 cut(s) 399
AccB7I CCANNNNNTGG 2 cut(s) 1169, 1360
AccII CGCG 2 cut(s) 1908, 1967
AciI CCGC 5 cut(s) 52, 79, 225, 1928, 2540
AcoI YGGCCR 2 cut(s) 264, 2043
AcsI RAATTY 5 cut(s) 217, 362, 1264, 1315, 2562
AcvI CACGTG 1 cut(s) 505
AcyI GRCGYC 1 cut(s) 1515
AfaI GTAC 5 cut(s) 10, 616, 1404, 1805, 2754
AflII CTTAAG 1 cut(s) 2110
AjiI CACGTC 1 cut(s) 1786
AjnI CCWGG 2 cut(s) 275, 491
AleI CACNNNNGTG 1 cut(s) 1079
AloI GAACNNNNNNTCC 2 cut(s) 408, 440
Alw21I GWGCWC 2 cut(s) 1047, 1953
AlwNI CAGNNNCTG 1 cut(s) 140
Ama87I CYCGRG 2 cut(s) 1946, 2757
AoxI GGCC 5 cut(s) 227, 264, 853, 1350, 2043
ApeKI GCWGC 7 cut(s) 488, 607, 610, 830, 1199, 2654, 2699
ApoI RAATTY 5 cut(s) 217, 362, 1264, 1315, 2562
Asp700I GAANNNNTTC 2 cut(s) 1022, 1335
AspLEI GCGC 4 cut(s) 434, 727, 1967, 1969
AspS9I GGNCC 5 cut(s) 227, 854, 1351, 2070, 2596
AsuC2I CCSGG 1 cut(s) 1652
AvaI CYCGRG 2 cut(s) 1946, 2757
AvaII GGWCC 2 cut(s) 2070, 2596
AxyI CCTNAGG 1 cut(s) 1710
BaeGI GKGCMC 2 cut(s) 504, 1657
BalI TGGCCA 1 cut(s) 2045
BamHI GGATCC 3 cut(s) 1159, 1860, 2334
BanI GGYRCC 1 cut(s) 399
BbrPI CACGTG 1 cut(s) 505
BbsI GAAGAC 1 cut(s) 1788
Bbv12I GWGCWC 2 cut(s) 1047, 1953
BbvCI CCTCAGC 1 cut(s) 102
BbvI GCAGC 7 cut(s) 500, 597, 619, 842, 1211, 2666, 2711
BceAI ACGGC 2 cut(s) 279, 419
BciT130I CCWGG 2 cut(s) 277, 493
BcnI CCSGG 1 cut(s) 1652
BfaI CTAG 9 cut(s) 149, 419, 788, 986, 1127, 1203, 1436, 2037, 2126
BfmI CTRYAG 2 cut(s) 12, 813
BfoI RGCGCY 2 cut(s) 435, 728
BfrI CTTAAG 1 cut(s) 2110
BfuAI ACCTGC 3 cut(s) 5, 148, 2179
BglI GCCNNNNNGGC 1 cut(s) 281
BglII AGATCT 2 cut(s) 421, 547
BisI GCNGC 7 cut(s) 489, 608, 611, 831, 1200, 2655, 2700
BlsI GCNGC 7 cut(s) 490, 609, 612, 832, 1201, 2656, 2701
BmcAI AGTACT 1 cut(s) 10
Bme1390I CCNGG 3 cut(s) 277, 493, 1652
Bme18I GGWCC 2 cut(s) 2070, 2596
BmeT110I CYCGRG 2 cut(s) 1946, 2757
BmgBI CACGTC 1 cut(s) 1786
BmgT120I GGNCC 5 cut(s) 227, 854, 1351, 2070, 2596
BmiI GGNNCC 5 cut(s) 401, 1161, 1862, 2072, 2336
BmrFI CCNGG 3 cut(s) 277, 493, 1652
BmrI ACTGGG 2 cut(s) 1859, 1903
BmsI GCATC 4 cut(s) 1683, 1845, 2208, 2755
BmuI ACTGGG 2 cut(s) 1859, 1903
BpiI GAAGAC 1 cut(s) 1788
BpmI CTGGAG 1 cut(s) 7
Bpu10I CCTNAGC 1 cut(s) 102
BpuEI CTTGAG 1 cut(s) 2659
BpuMI CCSGG 1 cut(s) 1652
Bsa29I ATCGAT 1 cut(s) 1595
BsaAI YACGTR 3 cut(s) 484, 505, 2321
BsaBI GATNNNNATC 1 cut(s) 1914
BsaHI GRCGYC 1 cut(s) 1515
BsaI GGTCTC 2 cut(s) 730, 1699
BsaJI CCNNGG 4 cut(s) 195, 276, 1163, 2756
BsaXI ACNNNNNCTCC 2 cut(s) 408, 438
Bse118I RCCGGY 1 cut(s) 2057
Bse1I ACTGG 3 cut(s) 24, 1865, 1898
Bse21I CCTNAGG 1 cut(s) 1710
Bse3DI GCAATG 1 cut(s) 2702
Bse8I GATNNNNATC 1 cut(s) 1914
BseBI CCWGG 2 cut(s) 277, 493
BseCI ATCGAT 1 cut(s) 1595
BseDI CCNNGG 4 cut(s) 195, 276, 1163, 2756
BseGI GGATG 5 cut(s) 1174, 1405, 1783, 1966, 2072
BseJI GATNNNNATC 1 cut(s) 1914
BseMI GCAATG 1 cut(s) 2702
BseMII CTCAG 4 cut(s) 93, 1701, 2181, 2268
BseNI ACTGG 3 cut(s) 24, 1865, 1898
BsePI GCGCGC 1 cut(s) 1965
BseSI GKGCMC 2 cut(s) 504, 1657
BseXI GCAGC 7 cut(s) 500, 597, 619, 842, 1211, 2666, 2711
BsgI GTGCAG 1 cut(s) 2718
Bsh1236I CGCG 2 cut(s) 1908, 1967
BshFI GGCC 5 cut(s) 229, 266, 855, 1352, 2045
BshNI GGYRCC 1 cut(s) 399
BshVI ATCGAT 1 cut(s) 1595
BsiHKAI GWGCWC 2 cut(s) 1047, 1953
BsiHKCI CYCGRG 2 cut(s) 1946, 2757
BsiSI CCGG 3 cut(s) 230, 1651, 2058
BslFI GGGAC 1 cut(s) 2056
BsmBI CGTCTC 1 cut(s) 450
BsmFI GGGAC 1 cut(s) 2056
BsmI GAATGC 2 cut(s) 196, 2105
BsnI GGCC 5 cut(s) 229, 266, 855, 1352, 2045
Bso31I GGTCTC 2 cut(s) 730, 1699
BsoBI CYCGRG 2 cut(s) 1946, 2757
Bsp1286I GDGCHC 4 cut(s) 504, 1047, 1657, 1953
Bsp1407I TGTACA 1 cut(s) 1402
Bsp68I TCGCGA 1 cut(s) 1908
BspACI CCGC 5 cut(s) 52, 79, 225, 1928, 2540
BspANI GGCC 5 cut(s) 229, 266, 855, 1352, 2045
BspCNI CTCAG 4 cut(s) 94, 1702, 2182, 2267
BspDI ATCGAT 1 cut(s) 1595
BspFNI CGCG 2 cut(s) 1908, 1967
BspHI TCATGA 2 cut(s) 1026, 1485
BspLI GGNNCC 5 cut(s) 401, 1161, 1862, 2072, 2336
BspMAI CTGCAG 1 cut(s) 16
BspMI ACCTGC 3 cut(s) 5, 148, 2179
BspQI GCTCTTC 6 cut(s) 588, 681, 786, 879, 1194, 1650
BspT107I GGYRCC 1 cut(s) 399
BspTI CTTAAG 1 cut(s) 2110
BspTNI GGTCTC 2 cut(s) 730, 1699
BsrDI GCAATG 1 cut(s) 2702
BsrFI RCCGGY 1 cut(s) 2057
BsrGI TGTACA 1 cut(s) 1402
BsrI ACTGG 3 cut(s) 24, 1865, 1898
BssAI RCCGGY 1 cut(s) 2057
BssECI CCNNGG 4 cut(s) 195, 276, 1163, 2756
BssHII GCGCGC 1 cut(s) 1965
BssNI GRCGYC 1 cut(s) 1515
BssT1I CCWWGG 2 cut(s) 195, 1163
Bst2UI CCWGG 2 cut(s) 277, 493
Bst4CI ACNGT 9 cut(s) 326, 619, 1015, 1371, 1634, 1733, 1876, 2288, 2626
Bst6I CTCTTC 7 cut(s) 588, 681, 711, 786, 879, 1194, 1650
BstACI GRCGYC 1 cut(s) 1515
BstAFI CTTAAG 1 cut(s) 2110
BstAPI GCANNNNNTGC 2 cut(s) 11, 2618
BstAUI TGTACA 1 cut(s) 1402
BstBAI YACGTR 3 cut(s) 484, 505, 2321
BstC8I GCNNGC 5 cut(s) 227, 1967, 2092, 2096, 2619
BstDEI CTNAG 6 cut(s) 102, 682, 1710, 1800, 2190, 2254
BstF5I GGATG 5 cut(s) 1174, 1405, 1783, 1966, 2072
BstFNI CGCG 2 cut(s) 1908, 1967
BstH2I RGCGCY 2 cut(s) 435, 728
BstHHI GCGC 4 cut(s) 434, 727, 1967, 1969
BstMWI GCNNNNNNNGC 6 cut(s) 11, 272, 281, 440, 2618, 2719
BstNI CCWGG 2 cut(s) 277, 493
BstNSI RCATGY 4 cut(s) 317, 1217, 2098, 2459
BstSCI CCNGG 3 cut(s) 275, 491, 1650
BstSFI CTRYAG 2 cut(s) 12, 813
BstSLI GKGCMC 2 cut(s) 504, 1657
BstUI CGCG 2 cut(s) 1908, 1967
BstV1I GCAGC 7 cut(s) 500, 597, 619, 842, 1211, 2666, 2711
BstV2I GAAGAC 1 cut(s) 1788
BstX2I RGATCY 8 cut(s) 106, 421, 448, 547, 1159, 1860, 2242, 2334
BstXI CCANNNNNNTGG 2 cut(s) 1081, 1396
BstYI RGATCY 8 cut(s) 106, 421, 448, 547, 1159, 1860, 2242, 2334
Bsu15I ATCGAT 1 cut(s) 1595
Bsu36I CCTNAGG 1 cut(s) 1710
BsuRI GGCC 5 cut(s) 229, 266, 855, 1352, 2045
BsuTUI ATCGAT 1 cut(s) 1595
BtgZI GCGATG 2 cut(s) 675, 1525
BtrI CACGTC 1 cut(s) 1786
BtsCI GGATG 5 cut(s) 1174, 1405, 1783, 1966, 2072
BtsI GCAGTG 3 cut(s) 867, 1182, 2619
BtsIMutI CAGTG 4 cut(s) 331, 867, 1182, 2619
BtuMI TCGCGA 1 cut(s) 1908
BveI ACCTGC 3 cut(s) 5, 148, 2179
Cac8I GCNNGC 5 cut(s) 227, 1967, 2092, 2096, 2619
CaiI CAGNNNCTG 1 cut(s) 140
CciI TCATGA 2 cut(s) 1026, 1485
CfoI GCGC 4 cut(s) 434, 727, 1967, 1969
Cfr10I RCCGGY 1 cut(s) 2057
Cfr13I GGNCC 5 cut(s) 227, 854, 1351, 2070, 2596
ClaI ATCGAT 1 cut(s) 1595
CseI GACGC 2 cut(s) 905, 1151
Csp6I GTAC 5 cut(s) 9, 615, 1403, 1804, 2753
CviQI GTAC 5 cut(s) 9, 615, 1403, 1804, 2753
DdeI CTNAG 6 cut(s) 102, 682, 1710, 1800, 2190, 2254
DrdI GACNNNNNNGTC 1 cut(s) 1608
DseDI GACNNNNNNGTC 1 cut(s) 1608
EaeI YGGCCR 2 cut(s) 264, 2043
Eam1104I CTCTTC 7 cut(s) 588, 681, 711, 786, 879, 1194, 1650
EarI CTCTTC 7 cut(s) 588, 681, 711, 786, 879, 1194, 1650
Eco130I CCWWGG 2 cut(s) 195, 1163
Eco31I GGTCTC 2 cut(s) 730, 1699
Eco32I GATATC 1 cut(s) 1239
Eco47I GGWCC 2 cut(s) 2070, 2596
Eco72I CACGTG 1 cut(s) 505
Eco81I CCTNAGG 1 cut(s) 1710
Eco88I CYCGRG 2 cut(s) 1946, 2757
EcoO109I RGGNCCY 1 cut(s) 854
EcoRII CCWGG 2 cut(s) 275, 491
EcoRV GATATC 1 cut(s) 1239
EcoT14I CCWWGG 2 cut(s) 195, 1163
EcoT22I ATGCAT 1 cut(s) 2770
ErhI CCWWGG 2 cut(s) 195, 1163
Esp3I CGTCTC 1 cut(s) 450
FaqI GGGAC 1 cut(s) 2056
FauI CCCGC 2 cut(s) 72, 218
FauNDI CATATG 1 cut(s) 1671
Fnu4HI GCNGC 7 cut(s) 489, 608, 611, 831, 1200, 2655, 2700
FokI GGATG 5 cut(s) 1181, 1412, 1790, 1973, 2079
Fsp4HI GCNGC 7 cut(s) 489, 608, 611, 831, 1200, 2655, 2700
FspBI CTAG 9 cut(s) 149, 419, 788, 986, 1127, 1203, 1436, 2037, 2126
GlaI GCGC 4 cut(s) 433, 726, 1966, 1968
GluI GCNGC 7 cut(s) 489, 608, 611, 831, 1200, 2655, 2700
GsuI CTGGAG 1 cut(s) 7
HaeII RGCGCY 2 cut(s) 435, 728
HaeIII GGCC 5 cut(s) 229, 266, 855, 1352, 2045
HapII CCGG 3 cut(s) 230, 1651, 2058
HgaI GACGC 2 cut(s) 905, 1151
HhaI GCGC 4 cut(s) 434, 727, 1967, 1969
Hin1I GRCGYC 1 cut(s) 1515
Hin6I GCGC 4 cut(s) 432, 725, 1965, 1967
HinP1I GCGC 4 cut(s) 432, 725, 1965, 1967
HincII GTYRAC 3 cut(s) 306, 537, 2555
HindII GTYRAC 3 cut(s) 306, 537, 2555
HinfI GANTC 6 cut(s) 1304, 1323, 1999, 2194, 2307, 2604
HpaI GTTAAC 1 cut(s) 306
HpaII CCGG 3 cut(s) 230, 1651, 2058
Hpy166II GTNNAC 6 cut(s) 29, 306, 537, 706, 2159, 2555
Hpy188I TCNGA 2 cut(s) 552, 2509
Hpy8I GTNNAC 6 cut(s) 29, 306, 537, 706, 2159, 2555
Hpy99I CGWCG 2 cut(s) 1520, 1634
HpyCH4III ACNGT 9 cut(s) 326, 619, 1015, 1371, 1634, 1733, 1876, 2288, 2626
HpyCH4IV ACGT 7 cut(s) 207, 483, 504, 1515, 1785, 2320, 2362
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 272, 281, 440, 2618, 2719
HpyF3I CTNAG 6 cut(s) 102, 682, 1710, 1800, 2190, 2254
HpySE526I ACGT 7 cut(s) 207, 483, 504, 1515, 1785, 2320, 2362
Hsp92I GRCGYC 1 cut(s) 1515
HspAI GCGC 4 cut(s) 432, 725, 1965, 1967
KspAI GTTAAC 1 cut(s) 306
LguI GCTCTTC 6 cut(s) 588, 681, 786, 879, 1194, 1650
Lsp1109I GCAGC 7 cut(s) 500, 597, 619, 842, 1211, 2666, 2711
LweI GCATC 4 cut(s) 1683, 1845, 2208, 2755
MaeI CTAG 9 cut(s) 149, 419, 788, 986, 1127, 1203, 1436, 2037, 2126
MaeII ACGT 7 cut(s) 207, 483, 504, 1515, 1785, 2320, 2362
MaeIII GTNAC 7 cut(s) 665, 770, 863, 1139, 2316, 2467, 2643
MflI RGATCY 8 cut(s) 106, 421, 448, 547, 1159, 1860, 2242, 2334
MhlI GDGCHC 4 cut(s) 504, 1047, 1657, 1953
MlsI TGGCCA 1 cut(s) 2045
MluNI TGGCCA 1 cut(s) 2045
MlyI GAGTC 2 cut(s) 1332, 2188
MmeI TCCRAC 2 cut(s) 1345, 2078
Mox20I TGGCCA 1 cut(s) 2045
Mph1103I ATGCAT 1 cut(s) 2770
MroXI GAANNNNTTC 2 cut(s) 1022, 1335
MscI TGGCCA 1 cut(s) 2045
MseI TTAA 9 cut(s) 240, 305, 405, 1446, 1536, 1550, 2111, 2295, 2733
MslI CAYNNNNRTG 5 cut(s) 1079, 1563, 2454, 2478, 2691
Msp20I TGGCCA 1 cut(s) 2045
MspA1I CMGCKG 1 cut(s) 610
MspCI CTTAAG 1 cut(s) 2110
MspI CCGG 3 cut(s) 230, 1651, 2058
MspR9I CCNGG 3 cut(s) 277, 493, 1652
Mva1269I GAATGC 2 cut(s) 196, 2105
MvaI CCWGG 2 cut(s) 277, 493
MvnI CGCG 2 cut(s) 1908, 1967
MwoI GCNNNNNNNGC 6 cut(s) 11, 272, 281, 440, 2618, 2719
NciI CCSGG 1 cut(s) 1652
NdeI CATATG 1 cut(s) 1671
NlaIV GGNNCC 5 cut(s) 401, 1161, 1862, 2072, 2336
NmuCI GTSAC 3 cut(s) 665, 863, 1139
NruI TCGCGA 1 cut(s) 1908
NsiI ATGCAT 1 cut(s) 2770
NspI RCATGY 4 cut(s) 317, 1217, 2098, 2459
OliI CACNNNNGTG 1 cut(s) 1079
PaeI GCATGC 1 cut(s) 2098
PagI TCATGA 2 cut(s) 1026, 1485
PaqCI CACCTGC 1 cut(s) 5
PauI GCGCGC 1 cut(s) 1965
PciSI GCTCTTC 6 cut(s) 588, 681, 786, 879, 1194, 1650
PctI GAATGC 2 cut(s) 196, 2105
PdmI GAANNNNTTC 2 cut(s) 1022, 1335
PfeI GAWTC 4 cut(s) 1304, 1999, 2307, 2604
PflMI CCANNNNNTGG 2 cut(s) 1169, 1360
PkrI GCNGC 7 cut(s) 490, 609, 612, 832, 1201, 2656, 2701
PleI GAGTC 2 cut(s) 1331, 2188
PmaCI CACGTG 1 cut(s) 505
PmlI CACGTG 1 cut(s) 505
PpsI GAGTC 2 cut(s) 1331, 2188
Ppu21I YACGTR 3 cut(s) 484, 505, 2321
PsiI TTATAA 2 cut(s) 248, 2261
Psp6I CCWGG 2 cut(s) 275, 491
PspCI CACGTG 1 cut(s) 505
PspGI CCWGG 2 cut(s) 275, 491
PspN4I GGNNCC 5 cut(s) 401, 1161, 1862, 2072, 2336
PspPI GGNCC 5 cut(s) 227, 854, 1351, 2070, 2596
PstI CTGCAG 1 cut(s) 16
PstNI CAGNNNCTG 1 cut(s) 140
PsuI RGATCY 8 cut(s) 106, 421, 448, 547, 1159, 1860, 2242, 2334
PteI GCGCGC 1 cut(s) 1965
PvuII CAGCTG 1 cut(s) 610
RruI TCGCGA 1 cut(s) 1908
RsaI GTAC 5 cut(s) 10, 616, 1404, 1805, 2754
RsaNI GTAC 5 cut(s) 9, 615, 1403, 1804, 2753
RseI CAYNNNNRTG 5 cut(s) 1079, 1563, 2454, 2478, 2691
SapI GCTCTTC 6 cut(s) 588, 681, 786, 879, 1194, 1650
SaqAI TTAA 9 cut(s) 240, 305, 405, 1446, 1536, 1550, 2111, 2295, 2733
SatI GCNGC 7 cut(s) 489, 608, 611, 831, 1200, 2655, 2700
Sau96I GGNCC 5 cut(s) 227, 854, 1351, 2070, 2596
ScaI AGTACT 1 cut(s) 10
SchI GAGTC 2 cut(s) 1332, 2188
ScrFI CCNGG 3 cut(s) 277, 493, 1652
SduI GDGCHC 4 cut(s) 504, 1047, 1657, 1953
SfaNI GCATC 4 cut(s) 1683, 1845, 2208, 2755
SfcI CTRYAG 2 cut(s) 12, 813
SinI GGWCC 2 cut(s) 2070, 2596
SmiMI CAYNNNNRTG 5 cut(s) 1079, 1563, 2454, 2478, 2691
SmlI CTYRAG 2 cut(s) 2110, 2674
SmoI CTYRAG 2 cut(s) 2110, 2674
SphI GCATGC 1 cut(s) 2098
SsiI CCGC 5 cut(s) 52, 79, 225, 1928, 2540
SspI AATATT 3 cut(s) 511, 1393, 1462
SspMI CTAG 9 cut(s) 149, 419, 788, 986, 1127, 1203, 1436, 2037, 2126
StyD4I CCNGG 3 cut(s) 275, 491, 1650
StyI CCWWGG 2 cut(s) 195, 1163
TaaI ACNGT 9 cut(s) 326, 619, 1015, 1371, 1634, 1733, 1876, 2288, 2626
TaiI ACGT 7 cut(s) 210, 486, 507, 1518, 1788, 2323, 2365
TaqI TCGA 5 cut(s) 123, 1518, 1595, 2227, 2344
TatI WGTACW 3 cut(s) 8, 1402, 1803
TfiI GAWTC 4 cut(s) 1304, 1999, 2307, 2604
Tru1I TTAA 9 cut(s) 240, 305, 405, 1446, 1536, 1550, 2111, 2295, 2733
Tru9I TTAA 9 cut(s) 240, 305, 405, 1446, 1536, 1550, 2111, 2295, 2733
TscAI CASTG 4 cut(s) 331, 874, 1189, 2619
TseFI GTSAC 3 cut(s) 665, 863, 1139
TseI GCWGC 7 cut(s) 488, 607, 610, 830, 1199, 2654, 2699
Tsp45I GTSAC 3 cut(s) 665, 863, 1139
TspRI CASTG 4 cut(s) 331, 874, 1189, 2619
Van91I CCANNNNNTGG 2 cut(s) 1169, 1360
Vha464I CTTAAG 1 cut(s) 2110
VpaK11BI GGWCC 2 cut(s) 2070, 2596
XapI RAATTY 5 cut(s) 217, 362, 1264, 1315, 2562
XbaI TCTAGA 1 cut(s) 148
XceI RCATGY 4 cut(s) 317, 1217, 2098, 2459
XcmI CCANNNNNNNNNTGG 3 cut(s) 1361, 2083, 2585
XmnI GAANNNNTTC 2 cut(s) 1022, 1335
XspI CTAG 9 cut(s) 149, 419, 788, 986, 1127, 1203, 1436, 2037, 2126
ZraI GACGTC 1 cut(s) 1516
ZrmI AGTACT 1 cut(s) 10
Zsp2I ATGCAT 1 cut(s) 2770
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.