Rh7DG456300

protein ubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0013581
Physical Location & Seq
Reverse (-)
64894136 .. 64903454
9319 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG456300.1

Sequence Viewer

Length: 1272 bp
ATGCTTTTTTTCAAAATTCAAAATTCAAAACTCATACAATATGCAAAGCGCGACTTTGATAGCTATATTCAGTTCACCACTTATGTGATAAAAGGTGAATGGGATAATGCAAAGGAATGCCTTATAAAACTAGATGATCCTTTTGACGCAATAACGATAGTGGATCCAAGAGACGGATATGGAAACACTGCTCTTCATGTAGCAGCTAGAGAAGGGCATGTGCATATTGTGAAAGAATCGATATCATTGCTGAAGGTGAGACAAGAAAATTTGCAACTGAAAATCGTAGAAGAATTTGCAACTTTTGGTTCTGGTATAAATCTAGGCATCACTGAAGAAGTTCTCATGGAAAAGGCCAAACACATGGTTGAACAATACCAGAAAACACTTGCCACTATTTTGGGTCTACAAAATGCACGAGGTTCTACAGCCCTTCACATAGCAGTATCCAAGGGTAATATAGATATTGTGAAAGAGTTGGTGCCATTAATGAGAAAAGAAGATTTAGAGATAAAAGATGTTGAAGGATTCACAGCTCTTCATAATGCAGTCGAGGAGGGAAATTTAGATATTGTGAAAGAGTTAGTGCCAATGATGAGACCAGAAGGTTTGGAATTAAAAACAGCCAAGGGCAGCAATCCACTTCACCTTGCAGTCATGTTGGGGCATATGAATATTGCGAAAGAGGTGATGCCATTTATGAGAAAAGAAGCTTTGGATGAAAAAGACGGTCAAGGTTATACAGCTTTAGGATGTGCGTTAGATGTACTCAAGGATGAAGACGTGATGGAAATAGCCAAGTACATGGCTGAAAACAACAATAAAGTATTTGGCATCAAAGTGCCTACTACTAATTGGATTCCAGTTGTCTGGGTTATCGGTAGAGGCAAATGGGATTTGTGTCTCTATCTCTATGCTCTTACTCCGCTTGAGTATCTAATGCCCGAGCGCGGTAAGGATGGCGCGCACTTGGTTATCCGGTGCTTTAGAGGGAAGAAGTTTGATATTGCTTTGGACTTACTTCGGCGTTGCCCTAGATTGGCCATCAGTCCAACCGGCTTTGGATGTCCCCCTATAATGGAATTGGCTGGCATGCATTCTGCATTCTTAAGTGGAACCCAACTTGGATTTTGGCAATATTTGATCTATAATCGTGAGTCTTCTTCCCCTCTGTTGATATATACTAGCCTCTCACATATATATGCTCACACATTTAAATCAATTTTCTTTGAGTCTTTTTGTTTAGTTTTTACTTTCCCAACTGTAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

423

Amino Acids

47.79

Weight (kDa)

6.21

Isoelectric Point (pI)

33.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 134 - 196 4.1e-13 Ankyrin repeats (3 copies)
Ank_5 PF13857 168 - 218 4.5e-07 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 125
AccB1I GGYRCC 1 cut(s) 481
AccI GTMKAC 1 cut(s) 406
AccII CGCG 3 cut(s) 51, 951, 965
AciI CCGC 2 cut(s) 926, 951
AclWI GGATC 3 cut(s) 131, 158, 171
AcoI YGGCCR 1 cut(s) 1041
AcsI RAATTY 5 cut(s) 15, 22, 268, 293, 562
AcuI CTGAAG 2 cut(s) 272, 354
AfaI GTAC 2 cut(s) 768, 803
AfiI CCNNNNNNNGG 4 cut(s) 173, 950, 1039, 1078
AflII CTTAAG 1 cut(s) 1108
AgsI TTSAA 5 cut(s) 13, 20, 27, 371, 524
AjiI CACGTC 1 cut(s) 784
AleI CACNNNNGTG 1 cut(s) 83
AluBI AGCT 5 cut(s) 63, 206, 536, 713, 746
AluI AGCT 5 cut(s) 63, 206, 536, 713, 746
Alw26I GTCTC 4 cut(s) 165, 253, 592, 908
AlwI GGATC 3 cut(s) 131, 158, 171
Ama87I CYCGRG 1 cut(s) 944
AoxI GGCC 2 cut(s) 354, 1041
ApeKI GCWGC 2 cut(s) 203, 633
ApoI RAATTY 5 cut(s) 15, 22, 268, 293, 562
AseI ATTAAT 1 cut(s) 488
Asp700I GAANNNNTTC 1 cut(s) 339
AspLEI GCGC 4 cut(s) 51, 951, 965, 967
AsuHPI GGTGA 5 cut(s) 67, 107, 268, 638, 700
AvaI CYCGRG 1 cut(s) 944
BalI TGGCCA 1 cut(s) 1043
BamHI GGATCC 1 cut(s) 163
BanI GGYRCC 1 cut(s) 481
BauI CACGAG 1 cut(s) 417
BbsI GAAGAC 2 cut(s) 786, 1152
BbvI GCAGC 2 cut(s) 215, 645
BccI CCATC 3 cut(s) 781, 953, 1052
BcgI CGANNNNNNTGC 2 cut(s) 229, 263
BciVI GTATCC 1 cut(s) 457
BcoDI GTCTC 4 cut(s) 165, 253, 592, 908
BfaI CTAG 5 cut(s) 131, 207, 323, 1035, 1185
BfmI CTRYAG 1 cut(s) 426
BfrI CTTAAG 1 cut(s) 1108
BfuI GTATCC 1 cut(s) 457
BisI GCNGC 2 cut(s) 204, 634
BlsI GCNGC 2 cut(s) 205, 635
BmeT110I CYCGRG 1 cut(s) 944
BmgBI CACGTC 1 cut(s) 784
BmiI GGNNCC 3 cut(s) 165, 483, 1117
BmsI GCATC 3 cut(s) 336, 681, 843
BpiI GAAGAC 2 cut(s) 786, 1152
BpuEI CTTGAG 2 cut(s) 755, 950
Bsa29I ATCGAT 1 cut(s) 239
BsaI GGTCTC 1 cut(s) 592
BsaJI CCNNGG 2 cut(s) 450, 627
BsaWI WCCGGW 1 cut(s) 978
Bsc4I CCNNNNNNNGG 4 cut(s) 173, 950, 1039, 1078
Bse118I RCCGGY 1 cut(s) 1055
Bse1I ACTGG 1 cut(s) 863
Bse3DI GCAATG 1 cut(s) 245
BseCI ATCGAT 1 cut(s) 239
BseDI CCNNGG 2 cut(s) 450, 627
BseGI GGATG 5 cut(s) 724, 758, 781, 964, 1070
BseLI CCNNNNNNNGG 4 cut(s) 173, 950, 1039, 1078
BseMI GCAATG 1 cut(s) 245
BseNI ACTGG 1 cut(s) 863
BsePI GCGCGC 1 cut(s) 963
BseRI GAGGAG 1 cut(s) 569
BseXI GCAGC 2 cut(s) 215, 645
Bsh1236I CGCG 3 cut(s) 51, 951, 965
BshFI GGCC 2 cut(s) 356, 1043
BshNI GGYRCC 1 cut(s) 481
BshVI ATCGAT 1 cut(s) 239
BsiHKCI CYCGRG 1 cut(s) 944
BsiSI CCGG 2 cut(s) 979, 1056
BslFI GGGAC 1 cut(s) 1053
BslI CCNNNNNNNGG 4 cut(s) 173, 950, 1039, 1078
BsmAI GTCTC 4 cut(s) 165, 253, 592, 908
BsmBI CGTCTC 1 cut(s) 165
BsmFI GGGAC 1 cut(s) 1053
BsmI GAATGC 3 cut(s) 122, 1096, 1103
BsnI GGCC 2 cut(s) 356, 1043
Bso31I GGTCTC 1 cut(s) 592
BsoBI CYCGRG 1 cut(s) 944
Bsp143I GATC 3 cut(s) 136, 163, 1143
BspACI CCGC 2 cut(s) 926, 951
BspANI GGCC 2 cut(s) 356, 1043
BspDI ATCGAT 1 cut(s) 239
BspFNI CGCG 3 cut(s) 51, 951, 965
BspLI GGNNCC 3 cut(s) 165, 483, 1117
BspPI GGATC 3 cut(s) 131, 158, 171
BspQI GCTCTTC 2 cut(s) 198, 543
BspT107I GGYRCC 1 cut(s) 481
BspTI CTTAAG 1 cut(s) 1108
BspTNI GGTCTC 1 cut(s) 592
BsrDI GCAATG 1 cut(s) 245
BsrFI RCCGGY 1 cut(s) 1055
BsrI ACTGG 1 cut(s) 863
BssAI RCCGGY 1 cut(s) 1055
BssECI CCNNGG 2 cut(s) 450, 627
BssHII GCGCGC 1 cut(s) 963
BssMI GATC 3 cut(s) 136, 163, 1143
BssSI CACGAG 1 cut(s) 417
BssT1I CCWWGG 2 cut(s) 450, 627
Bst2BI CACGAG 1 cut(s) 417
Bst4CI ACNGT 2 cut(s) 731, 1264
Bst6I CTCTTC 2 cut(s) 198, 543
BstAFI CTTAAG 1 cut(s) 1108
BstC8I GCNNGC 3 cut(s) 965, 1090, 1094
BstF5I GGATG 5 cut(s) 724, 758, 781, 964, 1070
BstFNI CGCG 3 cut(s) 51, 951, 965
BstHHI GCGC 4 cut(s) 51, 951, 965, 967
BstKTI GATC 3 cut(s) 139, 166, 1146
BstMAI GTCTC 4 cut(s) 165, 253, 592, 908
BstMBI GATC 3 cut(s) 136, 163, 1143
BstNSI RCATGY 2 cut(s) 221, 1096
BstSFI CTRYAG 1 cut(s) 426
BstUI CGCG 3 cut(s) 51, 951, 965
BstV1I GCAGC 2 cut(s) 215, 645
BstV2I GAAGAC 2 cut(s) 786, 1152
BstX2I RGATCY 1 cut(s) 163
BstXI CCANNNNNNTGG 4 cut(s) 364, 400, 805, 870
BstYI RGATCY 1 cut(s) 163
Bsu15I ATCGAT 1 cut(s) 239
BsuI GTATCC 1 cut(s) 457
BsuRI GGCC 2 cut(s) 356, 1043
BsuTUI ATCGAT 1 cut(s) 239
BtrI CACGTC 1 cut(s) 784
BtsCI GGATG 5 cut(s) 724, 758, 781, 964, 1070
BtsI GCAGTG 1 cut(s) 186
BtsIMutI CAGTG 2 cut(s) 186, 330
Cac8I GCNNGC 3 cut(s) 965, 1090, 1094
CfoI GCGC 4 cut(s) 51, 951, 965, 967
Cfr10I RCCGGY 1 cut(s) 1055
ClaI ATCGAT 1 cut(s) 239
CseI GACGC 1 cut(s) 155
Csp6I GTAC 2 cut(s) 767, 802
CviAII CATG 7 cut(s) 197, 218, 346, 364, 658, 805, 1093
CviQI GTAC 2 cut(s) 767, 802
DpnI GATC 3 cut(s) 138, 165, 1145
DpnII GATC 3 cut(s) 136, 163, 1143
DraI TTTAAA 1 cut(s) 1216
EaeI YGGCCR 1 cut(s) 1041
Eam1104I CTCTTC 2 cut(s) 198, 543
EarI CTCTTC 2 cut(s) 198, 543
Eco130I CCWWGG 2 cut(s) 450, 627
Eco31I GGTCTC 1 cut(s) 592
Eco32I GATATC 1 cut(s) 243
Eco57I CTGAAG 2 cut(s) 272, 354
Eco88I CYCGRG 1 cut(s) 944
EcoRV GATATC 1 cut(s) 243
EcoT14I CCWWGG 2 cut(s) 450, 627
EcoT22I ATGCAT 1 cut(s) 1098
ErhI CCWWGG 2 cut(s) 450, 627
Esp3I CGTCTC 1 cut(s) 165
FaeI CATG 7 cut(s) 200, 221, 349, 367, 661, 808, 1096
FalI AAGNNNNNCTT 2 cut(s) 38, 70
FaqI GGGAC 1 cut(s) 1053
FatI CATG 7 cut(s) 196, 217, 345, 363, 657, 804, 1092
FauNDI CATATG 1 cut(s) 669
FblI GTMKAC 1 cut(s) 406
Fnu4HI GCNGC 2 cut(s) 204, 634
FokI GGATG 5 cut(s) 731, 765, 788, 971, 1077
Fsp4HI GCNGC 2 cut(s) 204, 634
FspBI CTAG 5 cut(s) 131, 207, 323, 1035, 1185
GlaI GCGC 4 cut(s) 50, 950, 964, 966
GluI GCNGC 2 cut(s) 204, 634
HaeIII GGCC 2 cut(s) 356, 1043
HapII CCGG 2 cut(s) 979, 1056
HgaI GACGC 1 cut(s) 155
HhaI GCGC 4 cut(s) 51, 951, 965, 967
Hin1II CATG 7 cut(s) 200, 221, 349, 367, 661, 808, 1096
Hin6I GCGC 4 cut(s) 49, 949, 963, 965
HinP1I GCGC 4 cut(s) 49, 949, 963, 965
HindIII AAGCTT 1 cut(s) 711
HinfI GANTC 5 cut(s) 236, 528, 859, 1157, 1232
HpaII CCGG 2 cut(s) 979, 1056
HphI GGTGA 5 cut(s) 67, 107, 268, 638, 700
Hpy166II GTNNAC 2 cut(s) 75, 407
Hpy188III TCNNGA 1 cut(s) 1154
Hpy8I GTNNAC 2 cut(s) 75, 407
HpyAV CCTTC 5 cut(s) 206, 247, 443, 518, 599
HpyCH4III ACNGT 2 cut(s) 731, 1264
HpyCH4IV ACGT 1 cut(s) 783
HpySE526I ACGT 1 cut(s) 783
Hsp92II CATG 7 cut(s) 200, 221, 349, 367, 661, 808, 1096
HspAI GCGC 4 cut(s) 49, 949, 963, 965
Kzo9I GATC 3 cut(s) 136, 163, 1143
LguI GCTCTTC 2 cut(s) 198, 543
LpnPI CCDG 8 cut(s) 297, 392, 615, 856, 876, 992, 1069, 1074
Lsp1109I GCAGC 2 cut(s) 215, 645
LweI GCATC 3 cut(s) 336, 681, 843
MaeI CTAG 5 cut(s) 131, 207, 323, 1035, 1185
MaeII ACGT 1 cut(s) 783
MalI GATC 3 cut(s) 138, 165, 1145
MboI GATC 3 cut(s) 136, 163, 1143
MboII GAAGA 9 cut(s) 185, 302, 347, 512, 530, 791, 1006, 1152, 1155
MflI RGATCY 1 cut(s) 163
MlsI TGGCCA 1 cut(s) 1043
MluNI TGGCCA 1 cut(s) 1043
MlyI GAGTC 2 cut(s) 1166, 1241
MmeI TCCRAC 1 cut(s) 1076
MnlI CCTC 8 cut(s) 413, 547, 550, 679, 878, 983, 1179, 1199
Mox20I TGGCCA 1 cut(s) 1043
Mph1103I ATGCAT 1 cut(s) 1098
MroXI GAANNNNTTC 1 cut(s) 339
MscI TGGCCA 1 cut(s) 1043
MseI TTAA 5 cut(s) 488, 617, 1109, 1215, 1270
MslI CAYNNNNRTG 3 cut(s) 83, 839, 1200
Msp20I TGGCCA 1 cut(s) 1043
MspCI CTTAAG 1 cut(s) 1108
MspI CCGG 2 cut(s) 979, 1056
Mva1269I GAATGC 3 cut(s) 122, 1096, 1103
MvnI CGCG 3 cut(s) 51, 951, 965
NdeI CATATG 1 cut(s) 669
NdeII GATC 3 cut(s) 136, 163, 1143
NlaIII CATG 7 cut(s) 200, 221, 349, 367, 661, 808, 1096
NlaIV GGNNCC 3 cut(s) 165, 483, 1117
NsiI ATGCAT 1 cut(s) 1098
NspI RCATGY 2 cut(s) 221, 1096
OliI CACNNNNGTG 1 cut(s) 83
PaeI GCATGC 1 cut(s) 1096
PauI GCGCGC 1 cut(s) 963
PciSI GCTCTTC 2 cut(s) 198, 543
PctI GAATGC 3 cut(s) 122, 1096, 1103
PdmI GAANNNNTTC 1 cut(s) 339
PfeI GAWTC 3 cut(s) 236, 528, 859
PkrI GCNGC 2 cut(s) 205, 635
PleI GAGTC 2 cut(s) 1165, 1240
PpsI GAGTC 2 cut(s) 1165, 1240
PshBI ATTAAT 1 cut(s) 488
PsiI TTATAA 1 cut(s) 125
PspN4I GGNNCC 3 cut(s) 165, 483, 1117
PsuI RGATCY 1 cut(s) 163
PteI GCGCGC 1 cut(s) 963
RsaI GTAC 2 cut(s) 768, 803
RsaNI GTAC 2 cut(s) 767, 802
RseI CAYNNNNRTG 3 cut(s) 83, 839, 1200
SapI GCTCTTC 2 cut(s) 198, 543
SaqAI TTAA 5 cut(s) 488, 617, 1109, 1215, 1270
SatI GCNGC 2 cut(s) 204, 634
Sau3AI GATC 3 cut(s) 136, 163, 1143
SchI GAGTC 2 cut(s) 1166, 1241
SfaNI GCATC 3 cut(s) 336, 681, 843
SfcI CTRYAG 1 cut(s) 426
SmiI ATTTAAAT 1 cut(s) 1216
SmiMI CAYNNNNRTG 3 cut(s) 83, 839, 1200
SmlI CTYRAG 3 cut(s) 770, 929, 1108
SmoI CTYRAG 3 cut(s) 770, 929, 1108
SphI GCATGC 1 cut(s) 1096
SsiI CCGC 2 cut(s) 926, 951
SspI AATATT 2 cut(s) 676, 1139
SspMI CTAG 5 cut(s) 131, 207, 323, 1035, 1185
StyI CCWWGG 2 cut(s) 450, 627
SwaI ATTTAAAT 1 cut(s) 1216
TaaI ACNGT 2 cut(s) 731, 1264
TaiI ACGT 1 cut(s) 786
TaqI TCGA 2 cut(s) 239, 552
TatI WGTACW 2 cut(s) 766, 801
TfiI GAWTC 3 cut(s) 236, 528, 859
Tru1I TTAA 5 cut(s) 488, 617, 1109, 1215, 1270
Tru9I TTAA 5 cut(s) 488, 617, 1109, 1215, 1270
TscAI CASTG 2 cut(s) 193, 337
TseI GCWGC 2 cut(s) 203, 633
TspDTI ATGAA 5 cut(s) 185, 530, 686, 735, 792
TspGWI ACGGA 1 cut(s) 189
TspRI CASTG 2 cut(s) 193, 337
Vha464I CTTAAG 1 cut(s) 1108
VspI ATTAAT 1 cut(s) 488
XapI RAATTY 5 cut(s) 15, 22, 268, 293, 562
XceI RCATGY 2 cut(s) 221, 1096
XmiI GTMKAC 1 cut(s) 406
XmnI GAANNNNTTC 1 cut(s) 339
XspI CTAG 5 cut(s) 131, 207, 323, 1035, 1185
Zsp2I ATGCAT 1 cut(s) 1098
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.