Rw0G005500

protein ubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00219
OG0013581
Physical Location & Seq
Forward (+)
30385 .. 92162
61778 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G005500.1

Sequence Viewer

Length: 3246 bp
ATGGCAAGTTCTGCAGGTGTCTCCGGTTCACCTATTCTGGAAGTTCTCAACCGTAATAATTATAATAAGTGGAGATTGCGGTTAAAAACTTATTTGTTGGCTGAAGATTTATGGGAGGTTGTCGAAGCAATAACAGAACCTGCAAATCTAGAAGATGATGAAGCAGAATATAAATCTTGGAGAAAAAAGAATGCAAAGGCTTTATACGCAATCCAAAATTCATGCGGGCTAGAAATGTTTAATTTTATAAGCGAAACTGAAACAGCCAAAAGTGCCTGGGAGGCTTTGGAAAAAGTTTCGAGGTTAACAGGGCATGTTGAGAACAGTGCCGAAAACTTCGAGCCGTACGTACTGTTCACCAAATTTGTGACCGAAGGTGATTGGGATAAGGCAAAGGAGTGCCTTAAACTCAAAGAACTAGATCTCCACAGCGCCGTAAGAGCAATAGATCCAAGAGACGGGTTTGGAAACACAGCCATTCACGTAGCATCCTGTAAAGGGCACGTGAATATCGTGAGAGAGTTGGTGCTGTTGATGACCCAAGAAGATTTGAAAATGAAAAACGCTGAAGGTGCCACAGCTCTTCATACAGCAGCATTGAATGGGCACGTGAATATCGTGAGGGAGTTGGTGTTGTTGATGGGAGAAGAAAGAGACAGGGATGGTGACACAGCTCTTCACTTAGCAGTCTATAATGGGAAGGTGGACATTGTGAAAGAGTTGGTGCTGTTTATGAGACAAAAAGACTTGAAAATTCAAAACAACGACGGTTACACAGCTCTTCACCTAGCTGTTGAGAAGGGAAATGTGCCTATAGTGAAAGATTTAGCGGCGTTGGTGGGAGAAGTGAAAGGCCCTGATGGTGACACTGCTCTTCATATAGCAGTCAAGATGGGGGCTGCACAAATTGTGAGAGAGTTGGTGTTGTTGATGAGAAGGGAAGATTTGCCAATAAAAAATGATGAAGGCTACACAGCTTTTCACCTAGCAGTAAAGAAGGGTAATGTGCCTACTGTGAAAGAGTTCATGACAAAAGAAAAAGAGCGCAATTTTGATAGCTATGTTCAGTTCACCACTTATGTGGCAAATGGTGATTGGGATAATGCGAAGGAGTGCCTTACAAAACTAGATCATCCCTGTGACGCAATAAAAATAGTGGATCCAAGAGACGGATATGGAAACACCATTCTTCACGTAGCAGCTAGACAAGGGCATTTGGATATTGTGAAGGAATTGATTTCATTGCTGAAGGTGACACAAGAAAATTTGCAATTGAAAATTTTAGAAGACTTCGCAAATTTTGGTTCTGGTATAAATTTAGGCGTTCCTGAAAAAGTTTTCATAGAAAAGGCCCAATACATGGTTGAACAGTACGATAAAACACTTCCCAGTATTTTGGATGTACAAAACGCAGAAGGTTCCACGGCCCTTCACATAGCAGTATCCACGGGTAATTTAAATATTGTGAAAGAGTTGGTGCCCATGATGACACAAAAAGGTTTGGAATTAAAAACAGCTGGGGGCTCCAATGCACTTCACCTTGCAATCATGACCGGGCATATGAATATTGCAAAAGAGGTGATGCTATTTATGAGAAAAGGAGCTTTGGAAGAAAAGGATGGTGAAGGTTATACGGCTCTGGGTCGTGCGTTAGTTGTAAGCAAGGATGAAGACGTGATGGAAATAGCTGGGTACATGGCTGAAAACAACAAGGAAGTATTTGGCATCAACACGTCTCCCGGTAATTGGATTCCAGTTGTTAGTGCTACCATTAAGAGAGAATGGGATTTGTGTCGCTATCTCTATTCTCTTACTCCGCTTGAATATCTAATCCCCGAGAACGACACGGATGGCGCGCAGTTCATTATCGATTGCTTTAGAGCGAAGGAGCTTGATATGGCTTTGGACTTACTTCGGCGTTGCCCTAGATTGGCCATCAGTCCAACCAGCTATGGATGGCCCCCCATAATGGAATTGGCTGGCATGGATTCTGCATTCTTGAGTGGAACCCAACTTGGATTTTGGCAACATTTGATCTATAATCGTTTATGCATTGAACCTGCTAATTTGAACATTGCTCGTGATACTTGCATCAATATTTCAAAACAAGAGGATGATCGAGACAATCAAATGGGTCTCATTCGCTCAGTGGGGGTGATCCTGTTGAGTGGCTTAACAAGGCAGATCCGTACTTTGAGAGTCAATCGGTTACGTAAAGTGAAACGGATCCATACTCGAAGCATTGATGTTTTACGTTGCATTAGTAAAATGGTAAAGAATGACAATCCTACATCAACCGAACTTGACATCGTAAGAACATCAATCTTCAAAGCTGTTGAACACGGGCATGTTGAGTTTGTTACTCATATATGTGAAGCACATCCTCCATTCACAAAATCAAAAGAAGAAAACAAAAACATATTTCAGTTTGCGGTTGAATGTCGTCAACATAAAATGTATAGCCTTATTCATAGACTTGATAGAGAGGACAAGAGATTCTTTGGAATATGTGACACTGTGTTTCCAGAGAATATGTTACATTTGGCAGGGAAATTATCTCCGCCCTCAAGGTTCAATCATATTCGCGGTGCAGCTTTGCAAATGCAACGAGAGCTACAGTGGTTTAAGGAGGTAGAGGATATTGTACCTCAGGAGATGCATGGAATTTCAAATTCAGATGGTATGACACCACGTGAACTGTTTACCAAGAATCACGAGAACCTAAAAGAGGAGGCAGAAAAGTCGATGAAAGGGACAGCAACTTCTTGTACAGTTGTAGGCGCTCTCATCGTTACCATTATGTTTGCTGCAGCATTTACAGTTCCTGGTGGAAACAGTGGTAGTACAGGTTACCCGATGTTTCTAGCTAAGAAGTTGTTTCTGGTGTTCATAATTTCAGATACTCTATCACTTGTTTCTTCCACAACTTCAGTCATATTATTTCTGGGAATCCTTACATCACGTTATGCAGAAGATGATTTTCTCAAGAATTTGCCAACAAAGATGATGATAGGTCTTTTCACCCTTTTCCTCTCTATTGCCACCATGATGATTGCCTTTTCTTGTGCCCTCATCATTATGCTTGATGAAGAATACTCATGGGCCATTGTTCCTTGCATTTTTGCTGCTAGTGTTCCAGTTGCCTCATTTATATGGTTGCAATTTCCCCTCCTTATTGAGATATTCTGGTCAACTTATGGGCCAGGAATATTTGACAAGAAAGCCAAACCTTGGATTAAACTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1081

Amino Acids

121.34

Weight (kDa)

5.98

Isoelectric Point (pI)

32.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4219 PF13961 16 - 42 1.2e-08 Domain of unknown function (DUF4219)
Retrotran_gag_2 PF14223 29 - 98 5.9e-10 gag-polypeptide of LTR copia-type
Ank_2 PF12796 150 - 211 8.3e-11 Ankyrin repeats (3 copies)
Ank_2 PF12796 217 - 277 8e-12 Ankyrin repeats (3 copies)
Ank_5 PF13857 219 - 264 2.2e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 286 - 345 8.3e-08 Ankyrin repeats (3 copies)
Ank_5 PF13857 287 - 330 3.1e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 466 - 526 1.5e-09 Ankyrin repeats (3 copies)
PGG PF13962 914 - 1026 1.3e-23 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 63, 248
AarI CACCTGC 1 cut(s) 5
Acc36I ACCTGC 3 cut(s) 5, 148, 2068
AccB1I GGYRCC 2 cut(s) 572, 1477
AccB7I CCANNNNNTGG 2 cut(s) 1360, 3231
AccII CGCG 2 cut(s) 1856, 2586
AciI CCGC 7 cut(s) 79, 225, 830, 1817, 2432, 2561, 2586
AclWI GGATC 7 cut(s) 443, 1154, 1167, 2152, 2179, 2221, 2234
AcoI YGGCCR 1 cut(s) 1932
AcuI CTGAAG 4 cut(s) 123, 588, 1268, 2913
AcvI CACGTG 3 cut(s) 505, 610, 2693
AdeI CACNNNGTG 1 cut(s) 2693
AfaI GTAC 9 cut(s) 347, 351, 1373, 1404, 1694, 2191, 2646, 2770, 2845
AfiI CCNNNNNNNGG 9 cut(s) 458, 498, 1169, 1360, 1746, 1930, 1969, 2728, 3231
AflIII ACRYGT 1 cut(s) 1731
AjiI CACGTC 2 cut(s) 1675, 1734
AjnI CCWGG 3 cut(s) 275, 2824, 3202
AleI CACNNNNGTG 1 cut(s) 1079
AloI GAACNNNNNNTCC 2 cut(s) 408, 440
Alw26I GTCTC 8 cut(s) 25, 450, 648, 730, 1161, 1740, 2115, 2141
AlwI GGATC 7 cut(s) 443, 1154, 1167, 2152, 2179, 2221, 2234
AlwNI CAGNNNCTG 2 cut(s) 140, 2825
Ama87I CYCGRG 1 cut(s) 1835
AoxI GGCC 7 cut(s) 853, 1350, 1425, 1932, 1958, 3102, 3200
ApeKI GCWGC 7 cut(s) 593, 899, 1199, 2591, 2807, 2810, 3125
Asp700I GAANNNNTTC 1 cut(s) 1022
AspLEI GCGC 5 cut(s) 434, 1047, 1856, 1858, 2783
AspS9I GGNCC 6 cut(s) 854, 1351, 1426, 1959, 3102, 3200
AsuC2I CCSGG 2 cut(s) 1555, 1740
AvaI CYCGRG 1 cut(s) 1835
AxyI CCTNAGG 1 cut(s) 2649
BaeGI GKGCMC 4 cut(s) 504, 609, 1482, 3070
BalI TGGCCA 1 cut(s) 1934
BamHI GGATCC 2 cut(s) 1159, 2226
BanI GGYRCC 2 cut(s) 572, 1477
BanII GRGCYC 1 cut(s) 1526
BauI CACGAG 2 cut(s) 2079, 2714
BbrPI CACGTG 3 cut(s) 505, 610, 2693
BbsI GAAGAC 2 cut(s) 1293, 1677
BbvI GCAGC 7 cut(s) 605, 886, 1211, 2603, 2794, 2822, 3112
BceAI ACGGC 4 cut(s) 328, 419, 1440, 1650
BcgI CGANNNNNNTGC 2 cut(s) 2724, 2758
BciT130I CCWGG 3 cut(s) 277, 2826, 3204
BciVI GTATCC 1 cut(s) 1453
BcnI CCSGG 2 cut(s) 1555, 1740
BcoDI GTCTC 8 cut(s) 25, 450, 648, 730, 1161, 1740, 2115, 2141
BfmI CTRYAG 4 cut(s) 12, 813, 2615, 2808
BfoI RGCGCY 2 cut(s) 435, 2784
BfuAI ACCTGC 3 cut(s) 5, 148, 2068
BfuI GTATCC 1 cut(s) 1453
BglI GCCNNNNNGGC 1 cut(s) 281
BglII AGATCT 1 cut(s) 421
BisI GCNGC 8 cut(s) 594, 831, 900, 1200, 2592, 2808, 2811, 3126
BlsI GCNGC 8 cut(s) 595, 832, 901, 1201, 2593, 2809, 2812, 3127
Bme1390I CCNGG 5 cut(s) 277, 1555, 1740, 2826, 3204
BmeT110I CYCGRG 1 cut(s) 1835
BmgBI CACGTC 2 cut(s) 1675, 1734
BmgT120I GGNCC 6 cut(s) 854, 1351, 1426, 1959, 3102, 3200
BmiI GGNNCC 8 cut(s) 574, 1161, 1420, 1479, 1525, 1961, 2008, 2228
BmrFI CCNGG 5 cut(s) 277, 1555, 1740, 2826, 3204
BmrI ACTGGG 1 cut(s) 1383
BmsI GCATC 5 cut(s) 497, 1572, 1734, 2100, 2646
BmuI ACTGGG 1 cut(s) 1383
BpiI GAAGAC 2 cut(s) 1293, 1677
BpuEI CTTGAG 3 cut(s) 2020, 2551, 2969
BpuMI CCSGG 2 cut(s) 1555, 1740
Bsa29I ATCGAT 1 cut(s) 1869
BsaAI YACGTR 7 cut(s) 349, 484, 505, 610, 1195, 2213, 2693
BsaI GGTCTC 1 cut(s) 2141
BsaJI CCNNGG 4 cut(s) 276, 1422, 1446, 3230
BsaWI WCCGGW 1 cut(s) 23
BsaXI ACNNNNNCTCC 4 cut(s) 408, 438, 617, 647
Bsc4I CCNNNNNNNGG 9 cut(s) 458, 498, 1169, 1360, 1746, 1930, 1969, 2728, 3231
Bse1I ACTGG 3 cut(s) 1389, 1754, 3137
Bse21I CCTNAGG 1 cut(s) 2649
Bse3DI GCAATG 2 cut(s) 1241, 2073
BseBI CCWGG 3 cut(s) 277, 2826, 3204
BseCI ATCGAT 1 cut(s) 1869
BseDI CCNNGG 4 cut(s) 276, 1422, 1446, 3230
BseLI CCNNNNNNNGG 9 cut(s) 458, 498, 1169, 1360, 1746, 1930, 1969, 2728, 3231
BseMI GCAATG 2 cut(s) 1241, 2073
BseMII CTCAG 2 cut(s) 2160, 2663
BseNI ACTGG 3 cut(s) 1389, 1754, 3137
BsePI GCGCGC 1 cut(s) 1854
BseRI GAGGAG 1 cut(s) 2744
BseSI GKGCMC 4 cut(s) 504, 609, 1482, 3070
BseXI GCAGC 7 cut(s) 605, 886, 1211, 2603, 2794, 2822, 3112
BseYI CCCAGC 2 cut(s) 1517, 1688
BsgI GTGCAG 2 cut(s) 885, 2610
Bsh1236I CGCG 2 cut(s) 1856, 2586
BshFI GGCC 7 cut(s) 855, 1352, 1427, 1934, 1960, 3104, 3202
BshNI GGYRCC 2 cut(s) 572, 1477
BshVI ATCGAT 1 cut(s) 1869
BsiHKCI CYCGRG 1 cut(s) 1835
BsiSI CCGG 3 cut(s) 24, 1554, 1740
BsiWI CGTACG 1 cut(s) 345
BslFI GGGAC 1 cut(s) 2767
BslI CCNNNNNNNGG 9 cut(s) 458, 498, 1169, 1360, 1746, 1930, 1969, 2728, 3231
BsmAI GTCTC 8 cut(s) 25, 450, 648, 730, 1161, 1740, 2115, 2141
BsmBI CGTCTC 3 cut(s) 450, 1161, 1740
BsmFI GGGAC 1 cut(s) 2767
BsmI GAATGC 2 cut(s) 196, 1994
BsnI GGCC 7 cut(s) 855, 1352, 1427, 1934, 1960, 3104, 3202
Bso31I GGTCTC 1 cut(s) 2141
BsoBI CYCGRG 1 cut(s) 1835
Bsp1286I GDGCHC 5 cut(s) 504, 609, 1482, 1526, 3070
Bsp1407I TGTACA 2 cut(s) 1402, 2768
Bsp143I GATC 9 cut(s) 421, 448, 1129, 1159, 2034, 2116, 2157, 2184, 2226
BspACI CCGC 7 cut(s) 79, 225, 830, 1817, 2432, 2561, 2586
BspANI GGCC 7 cut(s) 855, 1352, 1427, 1934, 1960, 3104, 3202
BspCNI CTCAG 2 cut(s) 2159, 2662
BspDI ATCGAT 1 cut(s) 1869
BspFNI CGCG 2 cut(s) 1856, 2586
BspHI TCATGA 2 cut(s) 1026, 1548
BspLI GGNNCC 8 cut(s) 574, 1161, 1420, 1479, 1525, 1961, 2008, 2228
BspMAI CTGCAG 2 cut(s) 16, 2812
BspMI ACCTGC 3 cut(s) 5, 148, 2068
BspPI GGATC 7 cut(s) 443, 1154, 1167, 2152, 2179, 2221, 2234
BspQI GCTCTTC 4 cut(s) 588, 681, 786, 879
BspT107I GGYRCC 2 cut(s) 572, 1477
BspTNI GGTCTC 1 cut(s) 2141
BsrDI GCAATG 2 cut(s) 1241, 2073
BsrGI TGTACA 2 cut(s) 1402, 2768
BsrI ACTGG 3 cut(s) 1389, 1754, 3137
BssECI CCNNGG 4 cut(s) 276, 1422, 1446, 3230
BssHII GCGCGC 1 cut(s) 1854
BssMI GATC 9 cut(s) 421, 448, 1129, 1159, 2034, 2116, 2157, 2184, 2226
BssSI CACGAG 2 cut(s) 2079, 2714
BssT1I CCWWGG 1 cut(s) 3230
Bst2BI CACGAG 2 cut(s) 2079, 2714
Bst2UI CCWGG 3 cut(s) 277, 2826, 3204
Bst6I CTCTTC 4 cut(s) 588, 681, 786, 879
BstAPI GCANNNNNTGC 1 cut(s) 11
BstAUI TGTACA 2 cut(s) 1402, 2768
BstBAI YACGTR 7 cut(s) 349, 484, 505, 610, 1195, 2213, 2693
BstC8I GCNNGC 3 cut(s) 227, 1856, 1981
BstDEI CTNAG 4 cut(s) 682, 2146, 2649, 2868
BstDSI CCRYGG 2 cut(s) 1422, 1446
BstEII GGTNACC 1 cut(s) 2849
BstENI CCTNNNNNAGG 1 cut(s) 2726
BstFNI CGCG 2 cut(s) 1856, 2586
BstH2I RGCGCY 2 cut(s) 435, 2784
BstHHI GCGC 5 cut(s) 434, 1047, 1856, 1858, 2783
BstKTI GATC 9 cut(s) 424, 451, 1132, 1162, 2037, 2119, 2160, 2187, 2229
BstMAI GTCTC 8 cut(s) 25, 450, 648, 730, 1161, 1740, 2115, 2141
BstMBI GATC 9 cut(s) 421, 448, 1129, 1159, 2034, 2116, 2157, 2184, 2226
BstMWI GCNNNNNNNGC 7 cut(s) 11, 206, 272, 281, 440, 572, 2611
BstNI CCWGG 3 cut(s) 277, 2826, 3204
BstNSI RCATGY 2 cut(s) 317, 2351
BstPI GGTNACC 1 cut(s) 2849
BstSCI CCNGG 5 cut(s) 275, 1553, 1738, 2824, 3202
BstSFI CTRYAG 4 cut(s) 12, 813, 2615, 2808
BstSLI GKGCMC 4 cut(s) 504, 609, 1482, 3070
BstSNI TACGTA 2 cut(s) 349, 2213
BstUI CGCG 2 cut(s) 1856, 2586
BstV1I GCAGC 7 cut(s) 605, 886, 1211, 2603, 2794, 2822, 3112
BstV2I GAAGAC 2 cut(s) 1293, 1677
BstX2I RGATCY 5 cut(s) 421, 448, 1159, 2184, 2226
BstXI CCANNNNNNTGG 2 cut(s) 1081, 1396
BstYI RGATCY 5 cut(s) 421, 448, 1159, 2184, 2226
Bsu15I ATCGAT 1 cut(s) 1869
Bsu36I CCTNAGG 1 cut(s) 2649
BsuI GTATCC 1 cut(s) 1453
BsuRI GGCC 7 cut(s) 855, 1352, 1427, 1934, 1960, 3104, 3202
BsuTUI ATCGAT 1 cut(s) 1869
BtgI CCRYGG 2 cut(s) 1422, 1446
BtrI CACGTC 2 cut(s) 1675, 1734
BtsI GCAGTG 1 cut(s) 867
BtsIMutI CAGTG 6 cut(s) 331, 867, 2154, 2514, 2624, 2842
BveI ACCTGC 3 cut(s) 5, 148, 2068
Cac8I GCNNGC 3 cut(s) 227, 1856, 1981
CaiI CAGNNNCTG 2 cut(s) 140, 2825
CciI TCATGA 2 cut(s) 1026, 1548
CfoI GCGC 5 cut(s) 434, 1047, 1856, 1858, 2783
Cfr13I GGNCC 6 cut(s) 854, 1351, 1426, 1959, 3102, 3200
ClaI ATCGAT 1 cut(s) 1869
CseI GACGC 1 cut(s) 1151
Csp6I GTAC 9 cut(s) 346, 350, 1372, 1403, 1693, 2190, 2645, 2769, 2844
CviQI GTAC 9 cut(s) 346, 350, 1372, 1403, 1693, 2190, 2645, 2769, 2844
DdeI CTNAG 4 cut(s) 682, 2146, 2649, 2868
DpnI GATC 9 cut(s) 423, 450, 1131, 1161, 2036, 2118, 2159, 2186, 2228
DpnII GATC 9 cut(s) 421, 448, 1129, 1159, 2034, 2116, 2157, 2184, 2226
DraI TTTAAA 1 cut(s) 1458
DraIII CACNNNGTG 1 cut(s) 2693
EaeI YGGCCR 1 cut(s) 1932
Eam1104I CTCTTC 4 cut(s) 588, 681, 786, 879
EarI CTCTTC 4 cut(s) 588, 681, 786, 879
EciI GGCGGA 1 cut(s) 2550
Eco105I TACGTA 2 cut(s) 349, 2213
Eco130I CCWWGG 1 cut(s) 3230
Eco24I GRGCYC 1 cut(s) 1526
Eco31I GGTCTC 1 cut(s) 2141
Eco57I CTGAAG 4 cut(s) 123, 588, 1268, 2913
Eco72I CACGTG 3 cut(s) 505, 610, 2693
Eco81I CCTNAGG 1 cut(s) 2649
Eco88I CYCGRG 1 cut(s) 1835
Eco91I GGTNACC 1 cut(s) 2849
EcoNI CCTNNNNNAGG 1 cut(s) 2726
EcoO109I RGGNCCY 1 cut(s) 854
EcoO65I GGTNACC 1 cut(s) 2849
EcoRII CCWGG 3 cut(s) 275, 2824, 3202
EcoT14I CCWWGG 1 cut(s) 3230
EcoT22I ATGCAT 2 cut(s) 2054, 2661
EcoT38I GRGCYC 1 cut(s) 1526
ErhI CCWWGG 1 cut(s) 3230
Esp3I CGTCTC 3 cut(s) 450, 1161, 1740
FalI AAGNNNNNCTT 2 cut(s) 2483, 2515
FaqI GGGAC 1 cut(s) 2767
FauI CCCGC 1 cut(s) 218
FauNDI CATATG 1 cut(s) 1560
Fnu4HI GCNGC 8 cut(s) 594, 831, 900, 1200, 2592, 2808, 2811, 3126
FriOI GRGCYC 1 cut(s) 1526
Fsp4HI GCNGC 8 cut(s) 594, 831, 900, 1200, 2592, 2808, 2811, 3126
GlaI GCGC 5 cut(s) 433, 1046, 1855, 1857, 2782
GluI GCNGC 8 cut(s) 594, 831, 900, 1200, 2592, 2808, 2811, 3126
GsaI CCCAGC 2 cut(s) 1521, 1692
HaeII RGCGCY 2 cut(s) 435, 2784
HaeIII GGCC 7 cut(s) 855, 1352, 1427, 1934, 1960, 3104, 3202
HapII CCGG 3 cut(s) 24, 1554, 1740
HgaI GACGC 1 cut(s) 1151
HhaI GCGC 5 cut(s) 434, 1047, 1856, 1858, 2783
Hin6I GCGC 5 cut(s) 432, 1045, 1854, 1856, 2781
HinP1I GCGC 5 cut(s) 432, 1045, 1854, 1856, 2781
HincII GTYRAC 3 cut(s) 306, 2447, 3192
HindII GTYRAC 3 cut(s) 306, 2447, 3192
HinfI GANTC 6 cut(s) 1750, 1988, 2199, 2496, 2710, 2949
HpaI GTTAAC 1 cut(s) 306
HpaII CCGG 3 cut(s) 24, 1554, 1740
Hpy166II GTNNAC 9 cut(s) 29, 306, 357, 706, 1071, 2447, 2696, 2703, 3192
Hpy188I TCNGA 3 cut(s) 2677, 2899, 3245
Hpy8I GTNNAC 9 cut(s) 29, 306, 357, 706, 1071, 2447, 2696, 2703, 3192
Hpy99I CGWCG 1 cut(s) 770
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 206, 272, 281, 440, 572, 2611
HpyF3I CTNAG 4 cut(s) 682, 2146, 2649, 2868
HspAI GCGC 5 cut(s) 432, 1045, 1854, 1856, 2781
KspAI GTTAAC 1 cut(s) 306
Kzo9I GATC 9 cut(s) 421, 448, 1129, 1159, 2034, 2116, 2157, 2184, 2226
LguI GCTCTTC 4 cut(s) 588, 681, 786, 879
LmnI GCTCC 3 cut(s) 1529, 1601, 1888
Lsp1109I GCAGC 7 cut(s) 605, 886, 1211, 2603, 2794, 2822, 3112
LweI GCATC 5 cut(s) 497, 1572, 1734, 2100, 2646
MalI GATC 9 cut(s) 423, 450, 1131, 1161, 2036, 2118, 2159, 2186, 2228
MboI GATC 9 cut(s) 421, 448, 1129, 1159, 2034, 2116, 2157, 2184, 2226
MfeI CAATTG 1 cut(s) 1271
MflI RGATCY 5 cut(s) 421, 448, 1159, 2184, 2226
MhlI GDGCHC 5 cut(s) 504, 609, 1482, 1526, 3070
MlsI TGGCCA 1 cut(s) 1934
MluNI TGGCCA 1 cut(s) 1934
MlyI GAGTC 1 cut(s) 2208
MmeI TCCRAC 1 cut(s) 1967
Mox20I TGGCCA 1 cut(s) 1934
Mph1103I ATGCAT 2 cut(s) 2054, 2661
MroXI GAANNNNTTC 1 cut(s) 1022
MscI TGGCCA 1 cut(s) 1934
MslI CAYNNNNRTG 7 cut(s) 1079, 1137, 2346, 2370, 3047, 3077, 3151
Msp20I TGGCCA 1 cut(s) 1934
MspA1I CMGCKG 1 cut(s) 1517
MspI CCGG 3 cut(s) 24, 1554, 1740
MspR9I CCNGG 5 cut(s) 277, 1555, 1740, 2826, 3204
MunI CAATTG 1 cut(s) 1271
Mva1269I GAATGC 2 cut(s) 196, 1994
MvaI CCWGG 3 cut(s) 277, 2826, 3204
MvnI CGCG 2 cut(s) 1856, 2586
MwoI GCNNNNNNNGC 7 cut(s) 11, 206, 272, 281, 440, 572, 2611
NciI CCSGG 2 cut(s) 1555, 1740
NdeI CATATG 1 cut(s) 1560
NdeII GATC 9 cut(s) 421, 448, 1129, 1159, 2034, 2116, 2157, 2184, 2226
NlaIV GGNNCC 8 cut(s) 574, 1161, 1420, 1479, 1525, 1961, 2008, 2228
NmuCI GTSAC 6 cut(s) 367, 665, 863, 1139, 1252, 2510
NsiI ATGCAT 2 cut(s) 2054, 2661
NspI RCATGY 2 cut(s) 317, 2351
OliI CACNNNNGTG 1 cut(s) 1079
PagI TCATGA 2 cut(s) 1026, 1548
PaqCI CACCTGC 1 cut(s) 5
PauI GCGCGC 1 cut(s) 1854
PciSI GCTCTTC 4 cut(s) 588, 681, 786, 879
PcsI WCGNNNNNNNCGW 3 cut(s) 345, 510, 615
PctI GAATGC 2 cut(s) 196, 1994
PdmI GAANNNNTTC 1 cut(s) 1022
PfeI GAWTC 5 cut(s) 1750, 1988, 2496, 2710, 2949
Pfl23II CGTACG 1 cut(s) 345
PflMI CCANNNNNTGG 2 cut(s) 1360, 3231
PkrI GCNGC 8 cut(s) 595, 832, 901, 1201, 2593, 2809, 2812, 3127
PleI GAGTC 1 cut(s) 2207
PmaCI CACGTG 3 cut(s) 505, 610, 2693
PmlI CACGTG 3 cut(s) 505, 610, 2693
PpsI GAGTC 1 cut(s) 2207
Ppu21I YACGTR 7 cut(s) 349, 484, 505, 610, 1195, 2213, 2693
PsiI TTATAA 2 cut(s) 63, 248
Psp6I CCWGG 3 cut(s) 275, 2824, 3202
PspCI CACGTG 3 cut(s) 505, 610, 2693
PspEI GGTNACC 1 cut(s) 2849
PspFI CCCAGC 2 cut(s) 1517, 1688
PspGI CCWGG 3 cut(s) 275, 2824, 3202
PspLI CGTACG 1 cut(s) 345
PspN4I GGNNCC 8 cut(s) 574, 1161, 1420, 1479, 1525, 1961, 2008, 2228
PspPI GGNCC 6 cut(s) 854, 1351, 1426, 1959, 3102, 3200
PsrI GAACNNNNNNTAC 2 cut(s) 338, 370
PstI CTGCAG 2 cut(s) 16, 2812
PstNI CAGNNNCTG 2 cut(s) 140, 2825
PsuI RGATCY 5 cut(s) 421, 448, 1159, 2184, 2226
PteI GCGCGC 1 cut(s) 1854
PvuII CAGCTG 1 cut(s) 1517
RsaI GTAC 9 cut(s) 347, 351, 1373, 1404, 1694, 2191, 2646, 2770, 2845
RsaNI GTAC 9 cut(s) 346, 350, 1372, 1403, 1693, 2190, 2645, 2769, 2844
RseI CAYNNNNRTG 7 cut(s) 1079, 1137, 2346, 2370, 3047, 3077, 3151
SapI GCTCTTC 4 cut(s) 588, 681, 786, 879
SatI GCNGC 8 cut(s) 594, 831, 900, 1200, 2592, 2808, 2811, 3126
Sau3AI GATC 9 cut(s) 421, 448, 1129, 1159, 2034, 2116, 2157, 2184, 2226
Sau96I GGNCC 6 cut(s) 854, 1351, 1426, 1959, 3102, 3200
SchI GAGTC 1 cut(s) 2208
ScrFI CCNGG 5 cut(s) 277, 1555, 1740, 2826, 3204
SduI GDGCHC 5 cut(s) 504, 609, 1482, 1526, 3070
SfaNI GCATC 5 cut(s) 497, 1572, 1734, 2100, 2646
SfcI CTRYAG 4 cut(s) 12, 813, 2615, 2808
SmiI ATTTAAAT 1 cut(s) 1458
SmiMI CAYNNNNRTG 7 cut(s) 1079, 1137, 2346, 2370, 3047, 3077, 3151
SmlI CTYRAG 3 cut(s) 1999, 2566, 2984
SmoI CTYRAG 3 cut(s) 1999, 2566, 2984
SnaBI TACGTA 2 cut(s) 349, 2213
SsiI CCGC 7 cut(s) 79, 225, 830, 1817, 2432, 2561, 2586
SspI AATATT 4 cut(s) 1462, 1567, 2098, 3210
StyD4I CCNGG 5 cut(s) 275, 1553, 1738, 2824, 3202
StyI CCWWGG 1 cut(s) 3230
SwaI ATTTAAAT 1 cut(s) 1458
TaqI TCGA 7 cut(s) 123, 299, 339, 1869, 2119, 2236, 2744
TaqII GACCGA 1 cut(s) 386
TatI WGTACW 3 cut(s) 1402, 2768, 2843
TauI GCSGC 1 cut(s) 833
TfiI GAWTC 5 cut(s) 1750, 1988, 2496, 2710, 2949
TscAI CASTG 6 cut(s) 331, 874, 2154, 2521, 2624, 2842
TseFI GTSAC 6 cut(s) 367, 665, 863, 1139, 1252, 2510
TseI GCWGC 7 cut(s) 593, 899, 1199, 2591, 2807, 2810, 3125
Tsp45I GTSAC 6 cut(s) 367, 665, 863, 1139, 1252, 2510
TspGWI ACGGA 4 cut(s) 1185, 1862, 2177, 2239
TspRI CASTG 6 cut(s) 331, 874, 2154, 2521, 2624, 2842
Van91I CCANNNNNTGG 2 cut(s) 1360, 3231
XagI CCTNNNNNAGG 1 cut(s) 2726
XbaI TCTAGA 1 cut(s) 148
XceI RCATGY 2 cut(s) 317, 2351
XcmI CCANNNNNNNNNTGG 1 cut(s) 1972
XmnI GAANNNNTTC 1 cut(s) 1022
Zsp2I ATGCAT 2 cut(s) 2054, 2661
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.