Rw7G039310

protein ubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0013581
Physical Location & Seq
Forward (+)
60413416 .. 60450858
37443 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G039310.1

Sequence Viewer

Length: 3333 bp
ATGGCAAGTACTACAAGTGTCTCCAGTGCACCTATTCTAGAAGTTCTTAACCGTAATAACTACAAAAATTGGAGATTGCGGATAAAAACTTATTTGTTGGCTGAAGATCTATGGGAGGTTGTCGAAGCAATTAGGGAACCTGCTACAAAACTTGACGATAATGAACTTGAATACAAGGCTTGGAAACAAAAGAATGCTAAGGCTTTATATGCAATCCAAAATTCATGCGGGCATGAGATGTTTCGTTTTATAAGCGAAACTGAAACGGCCAAAAATGCCTGGGAGGCTTTAGAAAAAGCTTTGAGATTAACTGCAGGGCATGTTGAGAACCGTGCCGAAAAGTTCGAGCCCTACATACCGTTCACAGAATTTGTGAGCGAAGGTGATTGGGATAAGGCAAAGGAGTGCCTTAAACTCAAAGAACTAGATCTCCACAGCGCCGTAAGAGCAATAAATCCAAGAGACGGGTATGGAGACACAGCCATTCACGTAGCAGCGAGGAAAGGACATGTGAATATTGTGAAAGAGTTGGTGCTGTTGATGACCCAAGAAGATTTGAAAATGAAAAACGCTAAAGGTAATACAGCTCTTCATATAGCAGCATTGAATGGGCATCTGCGCACTGTGAAAGAGTTGGTGCTGTTGATGGGAGAAGAAATAGACAGCAATGGTGACACAGCTCTTCACTTAGCAGTCCATGGGGAGAAGGTGGACATTGTGAAAGAGTTGGCGCTGTTTATGAGACCAAAAGACTTGAAAATAAAAAACAACAATGGTTACACAGCTCTTCACCTAGCTGTTAAGAAGGGAAATGTGCCTATAGTGAAAGTTTTAGCGGCGTTGGTGGGAGAATTGAGAGGCCCTGATGGTGACACTGCTCTCCATATAGCAGTAAAGATGGGCGCTACACAAATTGTGAAAGAGTTGGTGTTGTTGATGAGAAGGGAAGATTTGGCAATAAAAAATGATGAAGGTTACACAGCCATTCACGTAGCAGCCAGGAAAGGGCATGTGAATATTGTGAAAGAGTTACGCAGTTTTGATAGATATACTCAGTTCATCAGTTATGTGGCAAATGGTGATTGGGATAATGCAAAGGAGTGCCTTACAAAACTAGATGATCCCTGTGACGCAATAACGATGGTGGATACAAGAGACGGATATGGAGACTCTGCTCTTCATGTAGCTGTTAGAAAAGGGCATGTGCATATTGTGAAAGAATTGATATCGTTGTTGAAGGTGAGAAAAGAAAATTTACAATTAAAAACCGTAGAAGATTTTGCAACTTTTGGTTCTTGTTTAAATTTAGACGTGACTGAAGAAGTTCTTATGGAAAGAGCCCAATACATGGTTGAACAGTACGAGAAAACATGTACCACTATTTTGGATGTACAAAATGCACAAGTTTCCACAGCCCTTCACATAGCAGTATCAAAGGGTAATTTAGATATTGTGAAAGAGTTGGTGTTATTAATGAGAAAAGAAGATTTAGAGATAAAAGATGCTGAAGGATTGACAGCTCTTCTCAATGCAGTCGACAAGGGTTATATTGATATTGTGAAAGAGTTGGTGCCAATTATGAGACAAGAAGGTTTGGAATTAAAAACAGCCAAGGGCAACAATACACTTCACCTTGCAGTCAGGTTGGGGCATATAAATATTGCGAAAGAGGTGATGCCATTTATGAGAAAAGGAGCTTTGGAAGAAAAAGATGGTGAAGGTTACACAGCTTTAGGTCGTGCGTTCGATGTAAGCAAGGATGAAGACGTGATGGAAATAGCCAAGTACATGGCTGAAAACAACAATAAAGTATTTGGCATCGGCGTGCCTCCTTATAATTGGATTCCAGTTGTCTGGGCTATCGTTAAGAGGAGATGGGAATTGTGTCGCTATCTCTATCCTCTTACTCCGCTTGAATATCTAATGCCTGAGAACTGGAGGCACGGCGCGCAGTTGATTATGGACTGCTTTAGAGCGAAGGAGCTTGATATGGCTTTGGACTTACTTAGGCGATGCCCTAGATTAGTCATCAGTCCAGCCGCCTCTGGTCCCCCCATAATGGAATTGGCGGGCATGCATTCAGCATTCTTGAGTGGAACGCAACTTGGATTTTGGCAACATTTGATCTACAATCGTTTACGCATTGAACCTGCTAATTTGGACACTTCTAATACTTACATCAATATTTCAAAACAAGAGGATGATCAAGACAATCAAATGGATCTCATTCGCTCAGTTATAACTTTATACAGGGAACTTGCATACAGTCTCCTTAAACTTTTGGGAGTCAATCGGTTACATAAAGTGAAATGGATCCATACTCGAAGCATTGATGTTTTACGTTGCATTAGTGAAATGCTAAAGAATGACAATCTTACATCAGCACAACTTGACATCGTAAGAACATCAATCTTCAAAGCTGTTGAGCACGGGCATGTTGAGTTTGTTACTCATATATGTGAAGCACATCCACCATTCGCAAAATCAAAGAAAGAAAACAAAAACATATTTCATTTTGCGGTTGAATATCGTCAACATAAAATTTATAGCCTTATTCATAGACTTGATGGAGAGGACAAGAGATTCTTTGCAATACGTTTAACCAAGTTTCCAGAGAATATGTTACATTTGGCAGGGAAATTATCTCCAATCTCAAGGTTCAATCGTATTCGTGGTGCAGTTTTGCAAATGCAACGAGAGCTACAGTGGTTTAAGGAGGTGGAGGATATTGTACCTGAAGAGATGCATGAAGTTCCAAATTCAGATGGTATGACACCACGTGAACTGTTTACCAAGAATCACGAGAACCTAAAAGAGGAGGCAGAAAAGTCTATGAAAGGGATAGCAACTTCTTGTACAGTTGTAGGCGCTCTCATTGTTACCATTATGTTTGCTGCAGCATTTACAGTTCCTGGTGGAAACGGTGGTAGTACAGGTTACCCGATGGTTCTTTCTAAGAAGTTGTTTCTGGTGTTCATAATTTCAGATACTCTATCACTCGTTTCTTCCACAACTTCAGTCATATTATTTTTGGGAATCCTCACATCACGTTATGCAGACGATGATTTTCTCAAGAATTTGCCAACAAAGATGATGATAGGCCTTTTCACCCTTTTTCTCTCTATTGCCACCATGATGATTGCCTTTTCTTCTGCCCTCATCATTATGCTTAAAAAAGAATACTCATGGGCCATTGGTCCTTGCATTTTTGTTGCTAGTATTCCAGTTGCCTCATTTATATGGTTTCAATTTCCCCTCCTTATTGAGATATTCTGGTCAACTTATGGACCAGGAATATTTGACAAGAAAGCCAAACCATGGATTAAACTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1110

Amino Acids

125.63

Weight (kDa)

7.07

Isoelectric Point (pI)

31.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4219 PF13961 16 - 42 1.6e-09 Domain of unknown function (DUF4219)
Retrotran_gag_2 PF14223 29 - 99 9e-11 gag-polypeptide of LTR copia-type
Ank_2 PF12796 151 - 221 8.8e-12 Ankyrin repeats (3 copies)
Ank_5 PF13857 151 - 200 2.3e-08 Ankyrin repeats (many copies)
Ank_2 PF12796 217 - 279 3.5e-11 Ankyrin repeats (3 copies)
Ank_2 PF12796 288 - 345 4.4e-10 Ankyrin repeats (3 copies)
Ank_5 PF13857 289 - 332 5e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 462 - 524 8.1e-09 Ankyrin repeats (3 copies)
Ank_5 PF13857 471 - 508 1.6e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 473 - 558 7.6e-13 Ankyrin repeats (3 copies)
PGG PF13962 943 - 1055 9e-23 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 251, 1836, 2240
Acc16I TGCGCA 1 cut(s) 620
Acc36I ACCTGC 2 cut(s) 148, 2158
AccB1I GGYRCC 1 cut(s) 1570
AccB7I CCANNNNNTGG 2 cut(s) 1348, 3318
AccI GTMKAC 1 cut(s) 1536
AccII CGCG 1 cut(s) 1949
AciI CCGC 7 cut(s) 79, 228, 836, 1910, 2040, 2069, 2519
AclWI GGATC 4 cut(s) 1115, 2229, 2308, 2321
AcoI YGGCCR 1 cut(s) 267
AcsI RAATTY 7 cut(s) 220, 368, 1252, 1303, 2541, 2758, 3076
AcuI CTGAAG 5 cut(s) 123, 1338, 1527, 2757, 3000
AcvI CACGTG 1 cut(s) 2780
AdeI CACNNNGTG 1 cut(s) 2780
AfaI GTAC 8 cut(s) 10, 1361, 1375, 1392, 1787, 2733, 2857, 2932
AfiI CCNNNNNNNGG 6 cut(s) 464, 1005, 1348, 2059, 2815, 3318
AflIII ACRYGT 2 cut(s) 508, 1370
AjiI CACGTC 2 cut(s) 1312, 1768
AjnI CCWGG 4 cut(s) 278, 998, 2911, 3289
AloI GAACNNNNNNTCC 2 cut(s) 414, 446
Alw21I GWGCWC 2 cut(s) 31, 2430
Alw26I GTCTC 8 cut(s) 25, 456, 468, 736, 1149, 1161, 1576, 2273
Alw44I GTGCAC 1 cut(s) 27
AlwI GGATC 4 cut(s) 1115, 2229, 2308, 2321
AlwNI CAGNNNCTG 1 cut(s) 2912
AoxI GGCC 4 cut(s) 267, 859, 3100, 3189
ApaLI GTGCAC 1 cut(s) 27
ApeKI GCWGC 5 cut(s) 494, 599, 995, 2894, 2897
ApoI RAATTY 7 cut(s) 220, 368, 1252, 1303, 2541, 2758, 3076
AseI ATTAAT 1 cut(s) 1472
Asp700I GAANNNNTTC 1 cut(s) 1323
AspLEI GCGC 7 cut(s) 440, 621, 733, 905, 1949, 1951, 2870
AspS9I GGNCC 5 cut(s) 860, 2048, 3189, 3197, 3287
AvaII GGWCC 3 cut(s) 2048, 3197, 3287
BaeGI GKGCMC 1 cut(s) 31
BamHI GGATCC 1 cut(s) 2313
BanI GGYRCC 1 cut(s) 1570
BanII GRGCYC 2 cut(s) 351, 1342
BauI CACGAG 1 cut(s) 2801
BbrPI CACGTG 1 cut(s) 2780
BbsI GAAGAC 1 cut(s) 1770
Bbv12I GWGCWC 2 cut(s) 31, 2430
BbvI GCAGC 5 cut(s) 506, 611, 1007, 2881, 2909
BceAI ACGGC 3 cut(s) 282, 425, 1960
BciT130I CCWGG 4 cut(s) 280, 1000, 2913, 3291
BciVI GTATCC 1 cut(s) 1141
BclI TGATCA 1 cut(s) 2203
BcoDI GTCTC 8 cut(s) 25, 456, 468, 736, 1149, 1161, 1576, 2273
BfaI CTAG 6 cut(s) 38, 425, 794, 1115, 2019, 3216
BfmI CTRYAG 4 cut(s) 312, 819, 2702, 2895
BfoI RGCGCY 4 cut(s) 441, 734, 906, 2871
BfuAI ACCTGC 2 cut(s) 148, 2158
BfuI GTATCC 1 cut(s) 1141
BglI GCCNNNNNGGC 1 cut(s) 284
BglII AGATCT 2 cut(s) 106, 427
BisI GCNGC 7 cut(s) 495, 600, 837, 996, 2040, 2895, 2898
BlsI GCNGC 7 cut(s) 496, 601, 838, 997, 2041, 2896, 2899
BmcAI AGTACT 1 cut(s) 10
Bme1390I CCNGG 4 cut(s) 280, 1000, 2913, 3291
Bme18I GGWCC 3 cut(s) 2048, 3197, 3287
BmgBI CACGTC 2 cut(s) 1312, 1768
BmgT120I GGNCC 5 cut(s) 860, 2048, 3189, 3197, 3287
BmiI GGNNCC 4 cut(s) 138, 1572, 2050, 2315
BmrFI CCNGG 4 cut(s) 280, 1000, 2913, 3291
BmsI GCATC 6 cut(s) 622, 1492, 1665, 1827, 2003, 2733
BpiI GAAGAC 1 cut(s) 1770
BpmI CTGGAG 2 cut(s) 7, 1957
Bpu10I CCTNAGC 1 cut(s) 198
BpuEI CTTGAG 3 cut(s) 2110, 2638, 3056
BsaAI YACGTR 3 cut(s) 490, 991, 2780
BsaI GGTCTC 1 cut(s) 736
BsaJI CCNNGG 4 cut(s) 279, 697, 1611, 3317
BsaXI ACNNNNNCTCC 2 cut(s) 414, 444
Bsc4I CCNNNNNNNGG 6 cut(s) 464, 1005, 1348, 2059, 2815, 3318
Bse1I ACTGG 4 cut(s) 24, 1847, 1940, 3224
Bse3DI GCAATG 1 cut(s) 673
BseBI CCWGG 4 cut(s) 280, 1000, 2913, 3291
BseDI CCNNGG 4 cut(s) 279, 697, 1611, 3317
BseGI GGATG 4 cut(s) 1393, 1765, 2206, 2467
BseLI CCNNNNNNNGG 6 cut(s) 464, 1005, 1348, 2059, 2815, 3318
BseMI GCAATG 1 cut(s) 673
BseMII CTCAG 3 cut(s) 1067, 1920, 2247
BseNI ACTGG 4 cut(s) 24, 1847, 1940, 3224
BsePI GCGCGC 1 cut(s) 1947
BseRI GAGGAG 2 cut(s) 1885, 2831
BseSI GKGCMC 1 cut(s) 31
BseXI GCAGC 5 cut(s) 506, 611, 1007, 2881, 2909
BsgI GTGCAG 1 cut(s) 2697
Bsh1236I CGCG 1 cut(s) 1949
BshFI GGCC 4 cut(s) 269, 861, 3102, 3191
BshNI GGYRCC 1 cut(s) 1570
BsiHKAI GWGCWC 2 cut(s) 31, 2430
BslFI GGGAC 1 cut(s) 2034
BslI CCNNNNNNNGG 6 cut(s) 464, 1005, 1348, 2059, 2815, 3318
BsmAI GTCTC 8 cut(s) 25, 456, 468, 736, 1149, 1161, 1576, 2273
BsmBI CGTCTC 2 cut(s) 456, 1149
BsmFI GGGAC 1 cut(s) 2034
BsmI GAATGC 3 cut(s) 199, 2077, 2084
BsnI GGCC 4 cut(s) 269, 861, 3102, 3191
Bso31I GGTCTC 1 cut(s) 736
Bsp1286I GDGCHC 4 cut(s) 31, 351, 1342, 2430
Bsp1407I TGTACA 2 cut(s) 1390, 2855
Bsp143I GATC 7 cut(s) 106, 427, 1120, 2124, 2203, 2221, 2313
Bsp19I CCATGG 2 cut(s) 697, 3317
BspACI CCGC 7 cut(s) 79, 228, 836, 1910, 2040, 2069, 2519
BspANI GGCC 4 cut(s) 269, 861, 3102, 3191
BspCNI CTCAG 3 cut(s) 1066, 1921, 2246
BspFNI CGCG 1 cut(s) 1949
BspLI GGNNCC 4 cut(s) 138, 1572, 2050, 2315
BspMAI CTGCAG 2 cut(s) 316, 2899
BspMI ACCTGC 2 cut(s) 148, 2158
BspPI GGATC 4 cut(s) 1115, 2229, 2308, 2321
BspQI GCTCTTC 5 cut(s) 594, 687, 792, 1182, 1527
BspT107I GGYRCC 1 cut(s) 1570
BspTNI GGTCTC 1 cut(s) 736
BsrDI GCAATG 1 cut(s) 673
BsrGI TGTACA 2 cut(s) 1390, 2855
BsrI ACTGG 4 cut(s) 24, 1847, 1940, 3224
BssECI CCNNGG 4 cut(s) 279, 697, 1611, 3317
BssHII GCGCGC 1 cut(s) 1947
BssMI GATC 7 cut(s) 106, 427, 1120, 2124, 2203, 2221, 2313
BssSI CACGAG 1 cut(s) 2801
BssT1I CCWWGG 3 cut(s) 697, 1611, 3317
Bst2BI CACGAG 1 cut(s) 2801
Bst2UI CCWGG 4 cut(s) 280, 1000, 2913, 3291
Bst6I CTCTTC 6 cut(s) 594, 687, 792, 1182, 1527, 2733
BstAUI TGTACA 2 cut(s) 1390, 2855
BstBAI YACGTR 3 cut(s) 490, 991, 2780
BstC8I GCNNGC 5 cut(s) 230, 1826, 1949, 2071, 2075
BstDEI CTNAG 7 cut(s) 198, 688, 1053, 1929, 2006, 2233, 2955
BstDSI CCRYGG 2 cut(s) 697, 3317
BstEII GGTNACC 1 cut(s) 2936
BstENI CCTNNNNNAGG 1 cut(s) 2813
BstF5I GGATG 4 cut(s) 1393, 1765, 2206, 2467
BstFNI CGCG 1 cut(s) 1949
BstH2I RGCGCY 4 cut(s) 441, 734, 906, 2871
BstHHI GCGC 7 cut(s) 440, 621, 733, 905, 1949, 1951, 2870
BstKTI GATC 7 cut(s) 109, 430, 1123, 2127, 2206, 2224, 2316
BstMAI GTCTC 8 cut(s) 25, 456, 468, 736, 1149, 1161, 1576, 2273
BstMBI GATC 7 cut(s) 106, 427, 1120, 2124, 2203, 2221, 2313
BstMWI GCNNNNNNNGC 6 cut(s) 209, 275, 284, 446, 1948, 2698
BstNI CCWGG 4 cut(s) 280, 1000, 2913, 3291
BstNSI RCATGY 7 cut(s) 323, 512, 1013, 1205, 1374, 2077, 2438
BstPI GGTNACC 1 cut(s) 2936
BstSCI CCNGG 4 cut(s) 278, 998, 2911, 3289
BstSFI CTRYAG 4 cut(s) 312, 819, 2702, 2895
BstSLI GKGCMC 1 cut(s) 31
BstUI CGCG 1 cut(s) 1949
BstV1I GCAGC 5 cut(s) 506, 611, 1007, 2881, 2909
BstV2I GAAGAC 1 cut(s) 1770
BstX2I RGATCY 4 cut(s) 106, 427, 2221, 2313
BstXI CCANNNNNNTGG 3 cut(s) 1384, 1789, 1854
BstYI RGATCY 4 cut(s) 106, 427, 2221, 2313
BsuI GTATCC 1 cut(s) 1141
BsuRI GGCC 4 cut(s) 269, 861, 3102, 3191
BtgI CCRYGG 2 cut(s) 697, 3317
BtgZI GCGATG 1 cut(s) 2026
BtrI CACGTC 2 cut(s) 1312, 1768
BtsCI GGATG 4 cut(s) 1393, 1765, 2206, 2467
BtsI GCAGTG 1 cut(s) 873
BtsIMutI CAGTG 4 cut(s) 31, 621, 873, 2711
BveI ACCTGC 2 cut(s) 148, 2158
Cac8I GCNNGC 5 cut(s) 230, 1826, 1949, 2071, 2075
CaiI CAGNNNCTG 1 cut(s) 2912
CfoI GCGC 7 cut(s) 440, 621, 733, 905, 1949, 1951, 2870
Cfr13I GGNCC 5 cut(s) 860, 2048, 3189, 3197, 3287
CseI GACGC 1 cut(s) 1139
Csp6I GTAC 8 cut(s) 9, 1360, 1374, 1391, 1786, 2732, 2856, 2931
CviQI GTAC 8 cut(s) 9, 1360, 1374, 1391, 1786, 2732, 2856, 2931
DdeI CTNAG 7 cut(s) 198, 688, 1053, 1929, 2006, 2233, 2955
DpnI GATC 7 cut(s) 108, 429, 1122, 2126, 2205, 2223, 2315
DpnII GATC 7 cut(s) 106, 427, 1120, 2124, 2203, 2221, 2313
DraI TTTAAA 1 cut(s) 1302
DraIII CACNNNGTG 1 cut(s) 2780
EaeI YGGCCR 1 cut(s) 267
Eam1104I CTCTTC 6 cut(s) 594, 687, 792, 1182, 1527, 2733
EarI CTCTTC 6 cut(s) 594, 687, 792, 1182, 1527, 2733
Eco130I CCWWGG 3 cut(s) 697, 1611, 3317
Eco147I AGGCCT 1 cut(s) 3102
Eco24I GRGCYC 2 cut(s) 351, 1342
Eco31I GGTCTC 1 cut(s) 736
Eco32I GATATC 1 cut(s) 1227
Eco47I GGWCC 3 cut(s) 2048, 3197, 3287
Eco57I CTGAAG 5 cut(s) 123, 1338, 1527, 2757, 3000
Eco72I CACGTG 1 cut(s) 2780
Eco91I GGTNACC 1 cut(s) 2936
EcoNI CCTNNNNNAGG 1 cut(s) 2813
EcoO109I RGGNCCY 1 cut(s) 860
EcoO65I GGTNACC 1 cut(s) 2936
EcoRII CCWGG 4 cut(s) 278, 998, 2911, 3289
EcoRV GATATC 1 cut(s) 1227
EcoT14I CCWWGG 3 cut(s) 697, 1611, 3317
EcoT22I ATGCAT 2 cut(s) 2079, 2748
EcoT38I GRGCYC 2 cut(s) 351, 1342
ErhI CCWWGG 3 cut(s) 697, 1611, 3317
Esp3I CGTCTC 2 cut(s) 456, 1149
FalI AAGNNNNNCTT 4 cut(s) 1311, 1343, 2570, 2602
FaqI GGGAC 1 cut(s) 2034
FauI CCCGC 2 cut(s) 221, 2062
FbaI TGATCA 1 cut(s) 2203
FblI GTMKAC 1 cut(s) 1536
Fnu4HI GCNGC 7 cut(s) 495, 600, 837, 996, 2040, 2895, 2898
FokI GGATG 4 cut(s) 1400, 1772, 2213, 2454
FriOI GRGCYC 2 cut(s) 351, 1342
Fsp4HI GCNGC 7 cut(s) 495, 600, 837, 996, 2040, 2895, 2898
FspBI CTAG 6 cut(s) 38, 425, 794, 1115, 2019, 3216
FspI TGCGCA 1 cut(s) 620
GlaI GCGC 7 cut(s) 439, 620, 732, 904, 1948, 1950, 2869
GluI GCNGC 7 cut(s) 495, 600, 837, 996, 2040, 2895, 2898
GsuI CTGGAG 2 cut(s) 7, 1957
HaeII RGCGCY 4 cut(s) 441, 734, 906, 2871
HaeIII GGCC 4 cut(s) 269, 861, 3102, 3191
HgaI GACGC 1 cut(s) 1139
HhaI GCGC 7 cut(s) 440, 621, 733, 905, 1949, 1951, 2870
Hin6I GCGC 7 cut(s) 438, 619, 731, 903, 1947, 1949, 2868
HinP1I GCGC 7 cut(s) 438, 619, 731, 903, 1947, 1949, 2868
HincII GTYRAC 3 cut(s) 1537, 2534, 3279
HindII GTYRAC 3 cut(s) 1537, 2534, 3279
HindIII AAGCTT 1 cut(s) 297
HinfI GANTC 6 cut(s) 1169, 1843, 2286, 2583, 2797, 3036
Hpy166II GTNNAC 9 cut(s) 29, 363, 712, 1537, 2138, 2534, 2783, 2790, 3279
Hpy188I TCNGA 2 cut(s) 2764, 2986
Hpy188III TCNNGA 6 cut(s) 38, 2089, 2207, 2612, 2801, 3073
Hpy8I GTNNAC 9 cut(s) 29, 363, 712, 1537, 2138, 2534, 2783, 2790, 3279
HpyCH4IV ACGT 8 cut(s) 489, 990, 1311, 1767, 2341, 2596, 2779, 3049
HpyF10VI GCNNNNNNNGC 6 cut(s) 209, 275, 284, 446, 1948, 2698
HpyF3I CTNAG 7 cut(s) 198, 688, 1053, 1929, 2006, 2233, 2955
HpySE526I ACGT 8 cut(s) 489, 990, 1311, 1767, 2341, 2596, 2779, 3049
HspAI GCGC 7 cut(s) 438, 619, 731, 903, 1947, 1949, 2868
Ksp22I TGATCA 1 cut(s) 2203
Kzo9I GATC 7 cut(s) 106, 427, 1120, 2124, 2203, 2221, 2313
LguI GCTCTTC 5 cut(s) 594, 687, 792, 1182, 1527
LmnI GCTCC 2 cut(s) 1694, 1981
Lsp1109I GCAGC 5 cut(s) 506, 611, 1007, 2881, 2909
LweI GCATC 6 cut(s) 622, 1492, 1665, 1827, 2003, 2733
MaeI CTAG 6 cut(s) 38, 425, 794, 1115, 2019, 3216
MaeII ACGT 8 cut(s) 489, 990, 1311, 1767, 2341, 2596, 2779, 3049
MalI GATC 7 cut(s) 108, 429, 1122, 2126, 2205, 2223, 2315
MboI GATC 7 cut(s) 106, 427, 1120, 2124, 2203, 2221, 2313
MflI RGATCY 4 cut(s) 106, 427, 2221, 2313
MhlI GDGCHC 4 cut(s) 31, 351, 1342, 2430
MlyI GAGTC 2 cut(s) 1163, 2295
Mph1103I ATGCAT 2 cut(s) 2079, 2748
MroXI GAANNNNTTC 1 cut(s) 1323
MslI CAYNNNNRTG 6 cut(s) 1823, 2433, 2457, 3134, 3164, 3238
MspR9I CCNGG 4 cut(s) 280, 1000, 2913, 3291
Mva1269I GAATGC 3 cut(s) 199, 2077, 2084
MvaI CCWGG 4 cut(s) 280, 1000, 2913, 3291
MvnI CGCG 1 cut(s) 1949
MwoI GCNNNNNNNGC 6 cut(s) 209, 275, 284, 446, 1948, 2698
NcoI CCATGG 2 cut(s) 697, 3317
NdeII GATC 7 cut(s) 106, 427, 1120, 2124, 2203, 2221, 2313
NlaIV GGNNCC 4 cut(s) 138, 1572, 2050, 2315
NmuCI GTSAC 4 cut(s) 671, 869, 1127, 1312
NsbI TGCGCA 1 cut(s) 620
NsiI ATGCAT 2 cut(s) 2079, 2748
NspI RCATGY 7 cut(s) 323, 512, 1013, 1205, 1374, 2077, 2438
PaeI GCATGC 1 cut(s) 2077
PauI GCGCGC 1 cut(s) 1947
PceI AGGCCT 1 cut(s) 3102
PciI ACATGT 2 cut(s) 508, 1370
PciSI GCTCTTC 5 cut(s) 594, 687, 792, 1182, 1527
PctI GAATGC 3 cut(s) 199, 2077, 2084
PdmI GAANNNNTTC 1 cut(s) 1323
PfeI GAWTC 4 cut(s) 1843, 2583, 2797, 3036
PflMI CCANNNNNTGG 2 cut(s) 1348, 3318
PkrI GCNGC 7 cut(s) 496, 601, 838, 997, 2041, 2896, 2899
PleI GAGTC 2 cut(s) 1163, 2294
PmaCI CACGTG 1 cut(s) 2780
PmlI CACGTG 1 cut(s) 2780
PpsI GAGTC 2 cut(s) 1163, 2294
Ppu21I YACGTR 3 cut(s) 490, 991, 2780
PscI ACATGT 2 cut(s) 508, 1370
PshBI ATTAAT 1 cut(s) 1472
PsiI TTATAA 3 cut(s) 251, 1836, 2240
Psp6I CCWGG 4 cut(s) 278, 998, 2911, 3289
PspCI CACGTG 1 cut(s) 2780
PspEI GGTNACC 1 cut(s) 2936
PspGI CCWGG 4 cut(s) 278, 998, 2911, 3289
PspN4I GGNNCC 4 cut(s) 138, 1572, 2050, 2315
PspPI GGNCC 5 cut(s) 860, 2048, 3189, 3197, 3287
PstI CTGCAG 2 cut(s) 316, 2899
PstNI CAGNNNCTG 1 cut(s) 2912
PsuI RGATCY 4 cut(s) 106, 427, 2221, 2313
PteI GCGCGC 1 cut(s) 1947
RsaI GTAC 8 cut(s) 10, 1361, 1375, 1392, 1787, 2733, 2857, 2932
RsaNI GTAC 8 cut(s) 9, 1360, 1374, 1391, 1786, 2732, 2856, 2931
RseI CAYNNNNRTG 6 cut(s) 1823, 2433, 2457, 3134, 3164, 3238
SalI GTCGAC 1 cut(s) 1535
SapI GCTCTTC 5 cut(s) 594, 687, 792, 1182, 1527
SatI GCNGC 7 cut(s) 495, 600, 837, 996, 2040, 2895, 2898
Sau3AI GATC 7 cut(s) 106, 427, 1120, 2124, 2203, 2221, 2313
Sau96I GGNCC 5 cut(s) 860, 2048, 3189, 3197, 3287
ScaI AGTACT 1 cut(s) 10
SchI GAGTC 2 cut(s) 1163, 2295
ScrFI CCNGG 4 cut(s) 280, 1000, 2913, 3291
SduI GDGCHC 4 cut(s) 31, 351, 1342, 2430
SfaNI GCATC 6 cut(s) 622, 1492, 1665, 1827, 2003, 2733
SfcI CTRYAG 4 cut(s) 312, 819, 2702, 2895
SinI GGWCC 3 cut(s) 2048, 3197, 3287
SmiMI CAYNNNNRTG 6 cut(s) 1823, 2433, 2457, 3134, 3164, 3238
SmlI CTYRAG 3 cut(s) 2089, 2653, 3071
SmoI CTYRAG 3 cut(s) 2089, 2653, 3071
SphI GCATGC 1 cut(s) 2077
SseBI AGGCCT 1 cut(s) 3102
SsiI CCGC 7 cut(s) 79, 228, 836, 1910, 2040, 2069, 2519
SspI AATATT 5 cut(s) 517, 1018, 1660, 2185, 3297
SspMI CTAG 6 cut(s) 38, 425, 794, 1115, 2019, 3216
StuI AGGCCT 1 cut(s) 3102
StyD4I CCNGG 4 cut(s) 278, 998, 2911, 3289
StyI CCWWGG 3 cut(s) 697, 1611, 3317
TaiI ACGT 8 cut(s) 492, 993, 1314, 1770, 2344, 2599, 2782, 3052
TaqI TCGA 5 cut(s) 123, 345, 1536, 1746, 2323
TatI WGTACW 5 cut(s) 8, 1390, 1785, 2855, 2930
TauI GCSGC 2 cut(s) 839, 2042
TfiI GAWTC 4 cut(s) 1843, 2583, 2797, 3036
TscAI CASTG 4 cut(s) 31, 628, 880, 2711
TseFI GTSAC 4 cut(s) 671, 869, 1127, 1312
TseI GCWGC 5 cut(s) 494, 599, 995, 2894, 2897
Tsp45I GTSAC 4 cut(s) 671, 869, 1127, 1312
TspGWI ACGGA 1 cut(s) 1173
TspRI CASTG 4 cut(s) 31, 628, 880, 2711
Van91I CCANNNNNTGG 2 cut(s) 1348, 3318
VneI GTGCAC 1 cut(s) 27
VpaK11BI GGWCC 3 cut(s) 2048, 3197, 3287
VspI ATTAAT 1 cut(s) 1472
XagI CCTNNNNNAGG 1 cut(s) 2813
XapI RAATTY 7 cut(s) 220, 368, 1252, 1303, 2541, 2758, 3076
XbaI TCTAGA 1 cut(s) 37
XceI RCATGY 7 cut(s) 323, 512, 1013, 1205, 1374, 2077, 2438
XcmI CCANNNNNNNNNTGG 1 cut(s) 2062
XmiI GTMKAC 1 cut(s) 1536
XmnI GAANNNNTTC 1 cut(s) 1323
XspI CTAG 6 cut(s) 38, 425, 794, 1115, 2019, 3216
ZrmI AGTACT 1 cut(s) 10
Zsp2I ATGCAT 2 cut(s) 2079, 2748
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.