RLG00000000892

protein ubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0013581
Physical Location & Seq
Forward (+)
7485197 .. 7534443
49247 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000000892

Sequence Viewer

Length: 2523 bp
ATGTCGGTGGATCGACGAATCTGGCGCTGCGGGGAGGCGATGGCTGTTGCTTGGATGGCCGCAGAAGAAGGATTGAAGGGGTCGGGTTACAGACCCATCTTTGCCGACGAGGGCGGGTCTAGATGGGTGTTGGAAAAGTGGCGGCGGTTCCGGTGGGGTGTCCTAGGGTTGTGGTTCCGGCGATCCAGTAGTGGCAGTGGTCGACGTTCCTACAGCGGGCATGTTGAGAACAGTGCCACAAACTTCAAGCCCTACATACCGTTCACCAAATTTGTGAGTGAAGGTGATTGGGATAAGGCAAAGGAGTGCCTTAAACTCGAAGAATTAGATCTCCACAGCGCCGTAAGAGCAAAAGATACAAGAGAAGAGTATGAAGACACAGCCATTCACGTAGCGGCACTCGAAGGGCATGTGAATATCGTGAAAGAGTTGGTGCTGTTGATGACCCAAGAAGATTTGAAAATGAAAAACGCTAAAGGTGACACAGCTCTTCATGTAGCAGCATGGAATGGGCGACTGAATGTTGTGAAGGAGTTGGTGCTGTTGATGGGAGAAGAAATAAACAGCGATGGTGACACAGCTCTTCACATAGCAGCCGATAGAGGGAAAGTGGATACTGTGAAAGAGTTGGTGCGGTTTATGAGACAAAAAGACTTGAAAAGAAAAAACAACAATGGTGACACAGCTCTTCACCTAGCAGTCAAGAAGGGAAATGTGCCTATAGTGAAAGATTTAGCCGAGTTGGTGGGAGAAGTGAGAGGCCCTGATGGTGACACTGCTCTTCACTTAGCAGTCAAGATGCTCGCTGCACAAATTGTGAAAGAGTTGGTGTTGTTGATGAGAAAGGAAGATTTGGCAATAAAAAATGACGAAGGTTACACAGCTTTTCACCTGGCAGTAAAGAAGGGTAATGTGCCTATTGTGAAAGAGTTCATGACAAAAGAAAAAGAGCGCGATTTTGATAGCTATATTCAGTTCACCACTTATGTGGCAAATGGTGATTGGCATAATGCAAAGGAGTGCCTTACAAAACTAGATGATCCCTGTGATGCAATAACGGTAGTGGATCCAAGAGACGGATATGGAAATAATGCTCTTCATGTAGCAGCTAAAGAAGGGCATGTGCATATTGTGAAAGAATCGATATCGTTGCTGAAGGTGAGACAAGAAACTTTACAACTGAAAATCGTAGAAGATTTCGCAACTTTTGGTTACGGTATAAATTTAGGTATCACTGAAGAAACTCTAATGGAAAGGGCCAAATACATGGTCGAACAGTACGACAAAACACTTACCAGTATTTTGGATGTACAAAATGCACAAAGTTCCACACCCCTTCACACAGCAATATCCGAGGGTAATTTAGATATTGTGAAAGAGTTGGTGCCAATGATGAGACCCGATGGTTTGGAATTAAAAACAGCCAAGGGCTCCAATGCACTTCACCTTGCAGTCAGGTTGGGGCATATGAATATTGCGAAAGAGTTGATGCCATTTATGAGAAAAGAAGCTTTGGAAGAAAAAGACGGTGGAGGCTATACAGCTTTAGGTCGTGCGTTAGGTGTACGCGAGAATGAAGACGTGAAGGATTTAGCCAAGTACATGGCTGAAAATAACAATAAAGTCTTTGGCGTCACTGTGCCTCCTTCTGATTGGATTCCAGTTATCAGGGCTCTCGATAAGAGCAAATGGGATTTGTGTTGCTATCTCTATCCTCTTACCCCGCTTGAATATCTAATGCCTGAGGCCGGGATGGATGGCGCACACTTGATCGTCAATTGCTTTAGAGCGAAGAAGCTTGATATGGCTTTGGACTTACTCCGGCGTTGCCCTAGATTGGCCATCTGTCCAACCGGCTTTGGATGTTCCCCCTTAATGGAATTGGCTGGCATGCATTCTGCATTCTTAAGTGGAACAGAACTAAGATTTTGGGAATATTTGATCTATAATCCTGTAACTTTATACAAGAAACTTGTATATAGTCTCCTTAAACTTATAGGAGTCAATCGAGTACGTAAAATGAAATGGATCCATACTCGAAGCATTGATGTTTTACGTTGCATTAGTGAAATGCTAAAAGAGAACAATCCTACATCAGCACAGCTTGACATCCTAAGAACATCAATTTTCAGAGCTGTAGAACACGGGAATGTTGAGTTTGTTACTCATATATGTGAAGCACATCCACCACTCATAAGATCATTTGAAAAAAGCAGAAACATATTTCAGCTTGCGGTTGAATGTCGTCAACATAAAGTTTATAGCCTTATACATAGACTTGATAAAGATGATCAGAGACACTTTGGAACACGTGTAACCCGGTTTCCAGAGAATATGTTACATTTGGCAGGGCAATTATCCCCACTCTCAAGATTCAATCGCATCCGCGGTGCAGCTTTGCAAATGCAACGAGAGCTACAGTGGTTTAAGGAGGTGGAGGGTATTGTACCTCTGGAGATGCAAGAAGTTTCAAATTCCGATGGAATATTCGATAAGAAAGCCAATCCATGGATTAAACTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

841

Amino Acids

95.24

Weight (kDa)

8.06

Isoelectric Point (pI)

33.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 119 - 188 6.1e-11 Ankyrin repeats (3 copies)
Ank_5 PF13857 123 - 167 7.4e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 182 - 247 2.4e-12 Ankyrin repeats (3 copies)
Ank_4 PF13637 195 - 245 3.1e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 255 - 314 3.4e-07 Ankyrin repeats (3 copies)
Ank_2 PF12796 435 - 497 2.4e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 446 - 540 2.5e-09 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1384
AccB7I CCANNNNNTGG 1 cut(s) 2508
AccI GTMKAC 1 cut(s) 202
AccII CGCG 3 cut(s) 954, 1569, 2388
AclWI GGATC 7 cut(s) 18, 177, 1034, 1061, 1074, 2023, 2036
AcoI YGGCCR 2 cut(s) 57, 1839
AcsI RAATTY 3 cut(s) 269, 1222, 2473
AcuI CTGAAG 2 cut(s) 1175, 1257
AcvI CACGTG 1 cut(s) 2312
AcyI GRCGYC 1 cut(s) 1632
AfaI GTAC 6 cut(s) 1280, 1311, 1566, 1601, 2013, 2448
AfiI CCNNNNNNNGG 7 cut(s) 216, 603, 1076, 1748, 1837, 1876, 2508
AflII CTTAAG 1 cut(s) 1906
AflIII ACRYGT 2 cut(s) 2309, 2311
AgsI TTSAA 9 cut(s) 76, 247, 460, 658, 1730, 2207, 2240, 2377, 2472
AjiI CACGTC 1 cut(s) 1582
AjnI CCWGG 1 cut(s) 891
AleI CACNNNNGTG 1 cut(s) 986
Alw26I GTCTC 6 cut(s) 637, 1068, 1156, 1390, 1988, 2290
AlwI GGATC 7 cut(s) 18, 177, 1034, 1061, 1074, 2023, 2036
AoxI GGCC 5 cut(s) 57, 760, 1257, 1746, 1839
ApeKI GCWGC 6 cut(s) 27, 500, 593, 806, 1106, 2393
ApoI RAATTY 3 cut(s) 269, 1222, 2473
ArsI GACNNNNNNTTYG 2 cut(s) 1254, 1286
Asp700I GAANNNNTTC 1 cut(s) 929
AspA2I CCTAGG 1 cut(s) 163
AspLEI GCGC 4 cut(s) 27, 341, 954, 1763
AspS9I GGNCC 2 cut(s) 761, 1257
AsuC2I CCSGG 2 cut(s) 1750, 2320
AvrII CCTAGG 1 cut(s) 163
AxyI CCTNAGG 1 cut(s) 1743
BalI TGGCCA 1 cut(s) 1841
BamHI GGATCC 2 cut(s) 1066, 2028
BanI GGYRCC 1 cut(s) 1384
BanII GRGCYC 2 cut(s) 1433, 1675
BbrPI CACGTG 1 cut(s) 2312
BbsI GAAGAC 2 cut(s) 381, 1584
BbvI GCAGC 6 cut(s) 14, 512, 605, 793, 1118, 2405
BceAI ACGGC 1 cut(s) 326
BcgI CGANNNNNNTGC 2 cut(s) 1132, 1166
BciT130I CCWGG 1 cut(s) 893
BciVI GTATCC 1 cut(s) 607
BclI TGATCA 1 cut(s) 2290
BcnI CCSGG 2 cut(s) 1750, 2320
BcoDI GTCTC 6 cut(s) 637, 1068, 1156, 1390, 1988, 2290
BfaI CTAG 5 cut(s) 120, 164, 695, 1034, 1833
BfmI CTRYAG 4 cut(s) 211, 720, 2136, 2417
BfoI RGCGCY 2 cut(s) 28, 342
BfrI CTTAAG 1 cut(s) 1906
BfuI GTATCC 1 cut(s) 607
BglII AGATCT 1 cut(s) 328
BisI GCNGC 9 cut(s) 28, 60, 143, 396, 501, 594, 807, 1107, 2394
BlnI CCTAGG 1 cut(s) 163
BlsI GCNGC 9 cut(s) 29, 61, 144, 397, 502, 595, 808, 1108, 2395
Bme1390I CCNGG 3 cut(s) 893, 1750, 2320
BmgBI CACGTC 1 cut(s) 1582
BmgT120I GGNCC 2 cut(s) 761, 1257
BmiI GGNNCC 6 cut(s) 149, 176, 1068, 1386, 1432, 2030
BmrFI CCNGG 3 cut(s) 893, 1750, 2320
BmsI GCATC 5 cut(s) 789, 1039, 1479, 2391, 2448
BpiI GAAGAC 2 cut(s) 381, 1584
BpmI CTGGAG 1 cut(s) 2474
BpuEI CTTGAG 1 cut(s) 2353
BpuMI CCSGG 2 cut(s) 1750, 2320
Bsa29I ATCGAT 1 cut(s) 1142
BsaAI YACGTR 3 cut(s) 391, 2015, 2312
BsaHI GRCGYC 1 cut(s) 1632
BsaI GGTCTC 1 cut(s) 1390
BsaJI CCNNGG 5 cut(s) 163, 1353, 1425, 2386, 2507
BsaWI WCCGGW 1 cut(s) 150
BsaXI ACNNNNNCTCC 2 cut(s) 1627, 1657
Bsc4I CCNNNNNNNGG 7 cut(s) 216, 603, 1076, 1748, 1837, 1876, 2508
Bse118I RCCGGY 1 cut(s) 1853
Bse1I ACTGG 3 cut(s) 186, 1296, 1661
Bse21I CCTNAGG 1 cut(s) 1743
BseBI CCWGG 1 cut(s) 893
BseCI ATCGAT 1 cut(s) 1142
BseDI CCNNGG 5 cut(s) 163, 1353, 1425, 2386, 2507
BseGI GGATG 8 cut(s) 60, 1312, 1758, 1762, 1868, 2109, 2182, 2382
BseLI CCNNNNNNNGG 7 cut(s) 216, 603, 1076, 1748, 1837, 1876, 2508
BseMII CTCAG 1 cut(s) 1734
BseNI ACTGG 3 cut(s) 186, 1296, 1661
BseXI GCAGC 6 cut(s) 14, 512, 605, 793, 1118, 2405
BsgI GTGCAG 2 cut(s) 792, 2412
Bsh1236I CGCG 3 cut(s) 954, 1569, 2388
BshFI GGCC 5 cut(s) 59, 762, 1259, 1748, 1841
BshNI GGYRCC 1 cut(s) 1384
BshVI ATCGAT 1 cut(s) 1142
BsiSI CCGG 6 cut(s) 151, 178, 1749, 1822, 1854, 2320
BslI CCNNNNNNNGG 7 cut(s) 216, 603, 1076, 1748, 1837, 1876, 2508
BsmAI GTCTC 6 cut(s) 637, 1068, 1156, 1390, 1988, 2290
BsmBI CGTCTC 1 cut(s) 1068
BsmI GAATGC 2 cut(s) 1894, 1901
BsnI GGCC 5 cut(s) 59, 762, 1259, 1748, 1841
Bso31I GGTCTC 1 cut(s) 1390
Bsp1286I GDGCHC 2 cut(s) 1433, 1675
Bsp1407I TGTACA 1 cut(s) 1309
Bsp19I CCATGG 1 cut(s) 2507
BspANI GGCC 5 cut(s) 59, 762, 1259, 1748, 1841
BspCNI CTCAG 1 cut(s) 1735
BspDI ATCGAT 1 cut(s) 1142
BspFNI CGCG 3 cut(s) 954, 1569, 2388
BspHI TCATGA 1 cut(s) 933
BspLI GGNNCC 6 cut(s) 149, 176, 1068, 1386, 1432, 2030
BspPI GGATC 7 cut(s) 18, 177, 1034, 1061, 1074, 2023, 2036
BspQI GCTCTTC 5 cut(s) 495, 588, 693, 786, 1101
BspT107I GGYRCC 1 cut(s) 1384
BspTI CTTAAG 1 cut(s) 1906
BspTNI GGTCTC 1 cut(s) 1390
BsrFI RCCGGY 1 cut(s) 1853
BsrGI TGTACA 1 cut(s) 1309
BsrI ACTGG 3 cut(s) 186, 1296, 1661
BssAI RCCGGY 1 cut(s) 1853
BssECI CCNNGG 5 cut(s) 163, 1353, 1425, 2386, 2507
BssNI GRCGYC 1 cut(s) 1632
BssT1I CCWWGG 3 cut(s) 163, 1425, 2507
Bst2UI CCWGG 1 cut(s) 893
Bst4CI ACNGT 9 cut(s) 233, 261, 619, 1060, 1217, 1278, 1529, 1639, 2421
Bst6I CTCTTC 6 cut(s) 360, 495, 588, 693, 786, 1101
BstACI GRCGYC 1 cut(s) 1632
BstAFI CTTAAG 1 cut(s) 1906
BstAUI TGTACA 1 cut(s) 1309
BstBAI YACGTR 3 cut(s) 391, 2015, 2312
BstC8I GCNNGC 5 cut(s) 218, 804, 1888, 1892, 2232
BstDEI CTNAG 4 cut(s) 787, 1743, 1922, 2114
BstDSI CCRYGG 2 cut(s) 2386, 2507
BstF5I GGATG 8 cut(s) 60, 1312, 1758, 1762, 1868, 2109, 2182, 2382
BstFNI CGCG 3 cut(s) 954, 1569, 2388
BstH2I RGCGCY 2 cut(s) 28, 342
BstHHI GCGC 4 cut(s) 27, 341, 954, 1763
BstMAI GTCTC 6 cut(s) 637, 1068, 1156, 1390, 1988, 2290
BstMWI GCNNNNNNNGC 3 cut(s) 56, 347, 2413
BstNI CCWGG 1 cut(s) 893
BstNSI RCATGY 4 cut(s) 224, 413, 1124, 1894
BstSCI CCNGG 3 cut(s) 891, 1748, 2318
BstSFI CTRYAG 4 cut(s) 211, 720, 2136, 2417
BstSNI TACGTA 1 cut(s) 2015
BstUI CGCG 3 cut(s) 954, 1569, 2388
BstV1I GCAGC 6 cut(s) 14, 512, 605, 793, 1118, 2405
BstV2I GAAGAC 2 cut(s) 381, 1584
BstX2I RGATCY 3 cut(s) 328, 1066, 2028
BstXI CCANNNNNNTGG 4 cut(s) 988, 1267, 1303, 1603
BstYI RGATCY 3 cut(s) 328, 1066, 2028
Bsu15I ATCGAT 1 cut(s) 1142
Bsu36I CCTNAGG 1 cut(s) 1743
BsuI GTATCC 1 cut(s) 607
BsuRI GGCC 5 cut(s) 59, 762, 1259, 1748, 1841
BsuTUI ATCGAT 1 cut(s) 1142
BtgI CCRYGG 2 cut(s) 2386, 2507
BtgZI GCGATG 2 cut(s) 53, 582
BtrI CACGTC 1 cut(s) 1582
BtsCI GGATG 8 cut(s) 60, 1312, 1758, 1762, 1868, 2109, 2182, 2382
BtsI GCAGTG 2 cut(s) 202, 774
BtsIMutI CAGTG 6 cut(s) 202, 238, 774, 1233, 1635, 2426
Cac8I GCNNGC 5 cut(s) 218, 804, 1888, 1892, 2232
CciI TCATGA 1 cut(s) 933
CfoI GCGC 4 cut(s) 27, 341, 954, 1763
Cfr10I RCCGGY 1 cut(s) 1853
Cfr13I GGNCC 2 cut(s) 761, 1257
Cfr42I CCGCGG 1 cut(s) 2389
ClaI ATCGAT 1 cut(s) 1142
CseI GACGC 1 cut(s) 1621
Csp6I GTAC 6 cut(s) 1279, 1310, 1565, 1600, 2012, 2447
CviQI GTAC 6 cut(s) 1279, 1310, 1565, 1600, 2012, 2447
DdeI CTNAG 4 cut(s) 787, 1743, 1922, 2114
EaeI YGGCCR 2 cut(s) 57, 1839
Eam1104I CTCTTC 6 cut(s) 360, 495, 588, 693, 786, 1101
EarI CTCTTC 6 cut(s) 360, 495, 588, 693, 786, 1101
Eco105I TACGTA 1 cut(s) 2015
Eco130I CCWWGG 3 cut(s) 163, 1425, 2507
Eco24I GRGCYC 2 cut(s) 1433, 1675
Eco31I GGTCTC 1 cut(s) 1390
Eco32I GATATC 1 cut(s) 1146
Eco57I CTGAAG 2 cut(s) 1175, 1257
Eco72I CACGTG 1 cut(s) 2312
Eco81I CCTNAGG 1 cut(s) 1743
EcoO109I RGGNCCY 1 cut(s) 761
EcoRII CCWGG 1 cut(s) 891
EcoRV GATATC 1 cut(s) 1146
EcoT14I CCWWGG 3 cut(s) 163, 1425, 2507
EcoT22I ATGCAT 1 cut(s) 1896
EcoT38I GRGCYC 2 cut(s) 1433, 1675
ErhI CCWWGG 3 cut(s) 163, 1425, 2507
Esp3I CGTCTC 1 cut(s) 1068
FauI CCCGC 4 cut(s) 23, 107, 209, 1731
FauNDI CATATG 1 cut(s) 1467
FbaI TGATCA 1 cut(s) 2290
FblI GTMKAC 1 cut(s) 202
Fnu4HI GCNGC 9 cut(s) 28, 60, 143, 396, 501, 594, 807, 1107, 2394
FokI GGATG 8 cut(s) 67, 1319, 1765, 1769, 1875, 2096, 2169, 2369
FriOI GRGCYC 2 cut(s) 1433, 1675
Fsp4HI GCNGC 9 cut(s) 28, 60, 143, 396, 501, 594, 807, 1107, 2394
FspBI CTAG 5 cut(s) 120, 164, 695, 1034, 1833
GlaI GCGC 4 cut(s) 26, 340, 953, 1762
GluI GCNGC 9 cut(s) 28, 60, 143, 396, 501, 594, 807, 1107, 2394
GsuI CTGGAG 1 cut(s) 2474
HaeII RGCGCY 2 cut(s) 28, 342
HaeIII GGCC 5 cut(s) 59, 762, 1259, 1748, 1841
HapII CCGG 6 cut(s) 151, 178, 1749, 1822, 1854, 2320
HgaI GACGC 1 cut(s) 1621
HhaI GCGC 4 cut(s) 27, 341, 954, 1763
Hin1I GRCGYC 1 cut(s) 1632
Hin6I GCGC 4 cut(s) 25, 339, 952, 1761
HinP1I GCGC 4 cut(s) 25, 339, 952, 1761
HincII GTYRAC 2 cut(s) 203, 2249
HindII GTYRAC 2 cut(s) 203, 2249
HindIII AAGCTT 2 cut(s) 1509, 1796
HinfI GANTC 5 cut(s) 18, 1139, 1657, 2001, 2373
HpaII CCGG 6 cut(s) 151, 178, 1749, 1822, 1854, 2320
Hpy166II GTNNAC 5 cut(s) 203, 264, 978, 1565, 2249
Hpy188I TCNGA 6 cut(s) 1354, 1651, 2132, 2295, 2479, 2522
Hpy188III TCNNGA 9 cut(s) 120, 421, 703, 796, 934, 1676, 2327, 2370, 2453
Hpy8I GTNNAC 5 cut(s) 203, 264, 978, 1565, 2249
Hpy99I CGWCG 3 cut(s) 18, 110, 207
HpyCH4III ACNGT 9 cut(s) 233, 261, 619, 1060, 1217, 1278, 1529, 1639, 2421
HpyCH4IV ACGT 6 cut(s) 205, 390, 1581, 2014, 2056, 2311
HpyF10VI GCNNNNNNNGC 3 cut(s) 56, 347, 2413
HpyF3I CTNAG 4 cut(s) 787, 1743, 1922, 2114
HpySE526I ACGT 6 cut(s) 205, 390, 1581, 2014, 2056, 2311
Hsp92I GRCGYC 1 cut(s) 1632
HspAI GCGC 4 cut(s) 25, 339, 952, 1761
Ksp22I TGATCA 1 cut(s) 2290
KspI CCGCGG 1 cut(s) 2389
LguI GCTCTTC 5 cut(s) 495, 588, 693, 786, 1101
LmnI GCTCC 1 cut(s) 1436
Lsp1109I GCAGC 6 cut(s) 14, 512, 605, 793, 1118, 2405
LweI GCATC 5 cut(s) 789, 1039, 1479, 2391, 2448
MaeI CTAG 5 cut(s) 120, 164, 695, 1034, 1833
MaeII ACGT 6 cut(s) 205, 390, 1581, 2014, 2056, 2311
MfeI CAATTG 1 cut(s) 1777
MflI RGATCY 3 cut(s) 328, 1066, 2028
MhlI GDGCHC 2 cut(s) 1433, 1675
MlsI TGGCCA 1 cut(s) 1841
MluNI TGGCCA 1 cut(s) 1841
MlyI GAGTC 1 cut(s) 2010
MmeI TCCRAC 2 cut(s) 111, 1874
Mox20I TGGCCA 1 cut(s) 1841
Mph1103I ATGCAT 1 cut(s) 1896
MroXI GAANNNNTTC 1 cut(s) 929
MscI TGGCCA 1 cut(s) 1841
MseI TTAA 7 cut(s) 312, 1415, 1874, 1907, 1989, 2427, 2514
MslI CAYNNNNRTG 3 cut(s) 986, 2148, 2172
Msp20I TGGCCA 1 cut(s) 1841
MspA1I CMGCKG 2 cut(s) 216, 2388
MspCI CTTAAG 1 cut(s) 1906
MspI CCGG 6 cut(s) 151, 178, 1749, 1822, 1854, 2320
MspR9I CCNGG 3 cut(s) 893, 1750, 2320
MunI CAATTG 1 cut(s) 1777
Mva1269I GAATGC 2 cut(s) 1894, 1901
MvaI CCWGG 1 cut(s) 893
MvnI CGCG 3 cut(s) 954, 1569, 2388
MwoI GCNNNNNNNGC 3 cut(s) 56, 347, 2413
NciI CCSGG 2 cut(s) 1750, 2320
NcoI CCATGG 1 cut(s) 2507
NdeI CATATG 1 cut(s) 1467
NlaIV GGNNCC 6 cut(s) 149, 176, 1068, 1386, 1432, 2030
NmeAIII GCCGAG 1 cut(s) 763
NmuCI GTSAC 5 cut(s) 479, 572, 677, 770, 1633
NsiI ATGCAT 1 cut(s) 1896
NspI RCATGY 4 cut(s) 224, 413, 1124, 1894
OliI CACNNNNGTG 1 cut(s) 986
PaeI GCATGC 1 cut(s) 1894
PagI TCATGA 1 cut(s) 933
PciSI GCTCTTC 5 cut(s) 495, 588, 693, 786, 1101
PcsI WCGNNNNNNNCGW 1 cut(s) 1278
PctI GAATGC 2 cut(s) 1894, 1901
PdmI GAANNNNTTC 1 cut(s) 929
PfeI GAWTC 4 cut(s) 18, 1139, 1657, 2373
PflMI CCANNNNNTGG 1 cut(s) 2508
PkrI GCNGC 9 cut(s) 29, 61, 144, 397, 502, 595, 808, 1108, 2395
PleI GAGTC 1 cut(s) 2009
PmaCI CACGTG 1 cut(s) 2312
PmlI CACGTG 1 cut(s) 2312
PpsI GAGTC 1 cut(s) 2009
Ppu21I YACGTR 3 cut(s) 391, 2015, 2312
Psp6I CCWGG 1 cut(s) 891
PspCI CACGTG 1 cut(s) 2312
PspGI CCWGG 1 cut(s) 891
PspN4I GGNNCC 6 cut(s) 149, 176, 1068, 1386, 1432, 2030
PspPI GGNCC 2 cut(s) 761, 1257
PsuI RGATCY 3 cut(s) 328, 1066, 2028
RsaI GTAC 6 cut(s) 1280, 1311, 1566, 1601, 2013, 2448
RsaNI GTAC 6 cut(s) 1279, 1310, 1565, 1600, 2012, 2447
RseI CAYNNNNRTG 3 cut(s) 986, 2148, 2172
SacII CCGCGG 1 cut(s) 2389
SalI GTCGAC 1 cut(s) 201
SapI GCTCTTC 5 cut(s) 495, 588, 693, 786, 1101
SaqAI TTAA 7 cut(s) 312, 1415, 1874, 1907, 1989, 2427, 2514
SatI GCNGC 9 cut(s) 28, 60, 143, 396, 501, 594, 807, 1107, 2394
Sau96I GGNCC 2 cut(s) 761, 1257
SchI GAGTC 1 cut(s) 2010
ScrFI CCNGG 3 cut(s) 893, 1750, 2320
SduI GDGCHC 2 cut(s) 1433, 1675
SfaNI GCATC 5 cut(s) 789, 1039, 1479, 2391, 2448
SfcI CTRYAG 4 cut(s) 211, 720, 2136, 2417
Sfr303I CCGCGG 1 cut(s) 2389
SgrBI CCGCGG 1 cut(s) 2389
SmiMI CAYNNNNRTG 3 cut(s) 986, 2148, 2172
SmlI CTYRAG 2 cut(s) 1906, 2368
SmoI CTYRAG 2 cut(s) 1906, 2368
SnaBI TACGTA 1 cut(s) 2015
SphI GCATGC 1 cut(s) 1894
SspI AATATT 3 cut(s) 1474, 1937, 2487
SspMI CTAG 5 cut(s) 120, 164, 695, 1034, 1833
StyD4I CCNGG 3 cut(s) 891, 1748, 2318
StyI CCWWGG 3 cut(s) 163, 1425, 2507
TaaI ACNGT 9 cut(s) 233, 261, 619, 1060, 1217, 1278, 1529, 1639, 2421
TaiI ACGT 6 cut(s) 208, 393, 1584, 2017, 2059, 2314
TatI WGTACW 2 cut(s) 1309, 1599
TauI GCSGC 3 cut(s) 62, 145, 398
TfiI GAWTC 4 cut(s) 18, 1139, 1657, 2373
Tru1I TTAA 7 cut(s) 312, 1415, 1874, 1907, 1989, 2427, 2514
Tru9I TTAA 7 cut(s) 312, 1415, 1874, 1907, 1989, 2427, 2514
TscAI CASTG 6 cut(s) 202, 238, 781, 1240, 1642, 2426
TseFI GTSAC 5 cut(s) 479, 572, 677, 770, 1633
TseI GCWGC 6 cut(s) 27, 500, 593, 806, 1106, 2393
Tsp45I GTSAC 5 cut(s) 479, 572, 677, 770, 1633
TspDTI ATGAA 8 cut(s) 387, 479, 482, 922, 1088, 1484, 1590, 2036
TspGWI ACGGA 1 cut(s) 1092
TspRI CASTG 6 cut(s) 202, 238, 781, 1240, 1642, 2426
Van91I CCANNNNNTGG 1 cut(s) 2508
Vha464I CTTAAG 1 cut(s) 1906
XapI RAATTY 3 cut(s) 269, 1222, 2473
XbaI TCTAGA 1 cut(s) 119
XceI RCATGY 4 cut(s) 224, 413, 1124, 1894
XmaJI CCTAGG 1 cut(s) 163
XmiI GTMKAC 1 cut(s) 202
XmnI GAANNNNTTC 1 cut(s) 929
XspI CTAG 5 cut(s) 120, 164, 695, 1034, 1833
Zsp2I ATGCAT 1 cut(s) 1896
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.