FvH4_1g03550

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0012408
Physical Location & Seq
Forward (+)
1938955 .. 1943920
4966 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g03550.t1

Sequence Viewer

Length: 597 bp
ATGTCGGGTAGCACAACACCAACTGGTGGATATATGAGGCAGAGGCATAGCCAGGGCTATGCGTCGAGTGGTGATGATCTTGAGGATGATGCGTGCTCTATGATGCATAGCTCGTCAGGTTCGAGTCCTAGGGTTTGGTCAAAGAGAGAGATTGCGGAGAATGTGATTTGGCTTGTCTCGGCAGCAGGCATTATGTACTTGGGTGATTCAAAGTCCAACTTGATATTTCTCTTGTGGCATGATGACCGAATAAGAAGATTGCCGTTGTACATTGGGATGGTGGGCGTGGTTTTGAATATAATCATCTTCTGTTACACAAGTATATCAGCATGGAGTGTTGGGCGATTTGATGAAAAATGGGAGTTAGCAAGTATATCTGCTCTGCCATATGTGACTCTACTTGGTGCCATTTCCTTTTGCCTGTTCTGCTTTGCTCTGTGGCCAATATGGGGATTCTTAACCCTTCCTCTTTTGTTCACGCTGTTTATGGCAGGCATGGTTATATTTCCATACATCATAATTGGGATATTTAGGAAACAACATGATGCGCTGCGTACAGATAATGCCTCCTCAGATTTTAGTGATAAACTATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

199

Amino Acids

22.25

Weight (kDa)

6.04

Isoelectric Point (pI)

47.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM128 PF20479 49 - 172 6.7e-30 TMEM128 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012408)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20120 AT5G20120
fragaria_vesca FvH4_1g03550
malus_domestica MD02G1034700.v1.1 MD15G1175500.v1.1
prunus_persica Prupe.7G240600_v2.0.a1
pyrus_communis pycom02g02830 pycom15g15700
rosa_chinensis RchiOBHm_Chr2g0089051
rosa_laevigata RLG00000015987
rosa_multiflora Rmu_sc0005510.1_g000007
rosa_roxburghii Rroxscaffold_2G00152300
rosa_rugosa Rorug01G0487000 Rorug01G0487100
rosa_samantha Rh2AG042500 Rh2BG041000 Rh2CG042600 Rh2DG041600
rosa_wichuraiana Rw2G003510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 404
AccB7I CCANNNNNTGG 1 cut(s) 26
AciI CCGC 1 cut(s) 155
AcoI YGGCCR 1 cut(s) 440
AfaI GTAC 3 cut(s) 197, 269, 556
AfiI CCNNNNNNNGG 2 cut(s) 26, 449
AgsI TTSAA 2 cut(s) 210, 295
AjnI CCWGG 1 cut(s) 51
AluBI AGCT 1 cut(s) 111
AluI AGCT 1 cut(s) 111
Alw21I GWGCWC 1 cut(s) 98
Alw26I GTCTC 1 cut(s) 181
AoxI GGCC 1 cut(s) 440
ApeKI GCWGC 2 cut(s) 182, 550
AspA2I CCTAGG 1 cut(s) 128
AspLEI GCGC 1 cut(s) 550
AsuHPI GGTGA 2 cut(s) 83, 215
AvrII CCTAGG 1 cut(s) 128
BalI TGGCCA 1 cut(s) 442
BanI GGYRCC 1 cut(s) 404
Bbv12I GWGCWC 1 cut(s) 98
BbvI GCAGC 2 cut(s) 194, 537
BccI CCATC 1 cut(s) 271
BceAI ACGGC 1 cut(s) 247
BciT130I CCWGG 1 cut(s) 53
BcoDI GTCTC 1 cut(s) 181
BfaI CTAG 1 cut(s) 129
BisI GCNGC 2 cut(s) 183, 551
BlnI CCTAGG 1 cut(s) 128
BlsI GCNGC 2 cut(s) 184, 552
Bme1390I CCNGG 1 cut(s) 53
BmiI GGNNCC 1 cut(s) 406
BmrFI CCNGG 1 cut(s) 53
BmsI GCATC 3 cut(s) 79, 93, 535
BpuEI CTTGAG 1 cut(s) 101
BsaJI CCNNGG 2 cut(s) 52, 128
Bsc4I CCNNNNNNNGG 2 cut(s) 26, 449
Bse1I ACTGG 1 cut(s) 28
BseBI CCWGG 1 cut(s) 53
BseDI CCNNGG 2 cut(s) 52, 128
BseGI GGATG 2 cut(s) 91, 282
BseLI CCNNNNNNNGG 2 cut(s) 26, 449
BseMII CTCAG 1 cut(s) 585
BseNI ACTGG 1 cut(s) 28
BseRI GAGGAG 1 cut(s) 559
BseXI GCAGC 2 cut(s) 194, 537
BshFI GGCC 1 cut(s) 442
BshNI GGYRCC 1 cut(s) 404
BsiHKAI GWGCWC 1 cut(s) 98
BslI CCNNNNNNNGG 2 cut(s) 26, 449
BsmAI GTCTC 1 cut(s) 181
BsnI GGCC 1 cut(s) 442
Bsp1286I GDGCHC 1 cut(s) 98
Bsp1407I TGTACA 1 cut(s) 267
Bsp143I GATC 1 cut(s) 76
BspACI CCGC 1 cut(s) 155
BspANI GGCC 1 cut(s) 442
BspCNI CTCAG 1 cut(s) 584
BspLI GGNNCC 1 cut(s) 406
BspT107I GGYRCC 1 cut(s) 404
BsrGI TGTACA 1 cut(s) 267
BsrI ACTGG 1 cut(s) 28
BssECI CCNNGG 2 cut(s) 52, 128
BssMI GATC 1 cut(s) 76
BssT1I CCWWGG 1 cut(s) 128
Bst2UI CCWGG 1 cut(s) 53
BstAUI TGTACA 1 cut(s) 267
BstC8I GCNNGC 3 cut(s) 94, 187, 493
BstDEI CTNAG 1 cut(s) 571
BstF5I GGATG 2 cut(s) 91, 282
BstHHI GCGC 1 cut(s) 550
BstKTI GATC 1 cut(s) 79
BstMAI GTCTC 1 cut(s) 181
BstMBI GATC 1 cut(s) 76
BstMWI GCNNNNNNNGC 1 cut(s) 426
BstNI CCWGG 1 cut(s) 53
BstSCI CCNGG 1 cut(s) 51
BstV1I GCAGC 2 cut(s) 194, 537
BsuRI GGCC 1 cut(s) 442
BtsCI GGATG 2 cut(s) 91, 282
Cac8I GCNNGC 3 cut(s) 94, 187, 493
CfoI GCGC 1 cut(s) 550
CseI GACGC 1 cut(s) 51
Csp6I GTAC 3 cut(s) 196, 268, 555
CviAII CATG 4 cut(s) 239, 330, 496, 542
CviJI RGCY 5 cut(s) 51, 57, 111, 172, 442
CviKI_1 RGCY 5 cut(s) 51, 57, 111, 172, 442
CviQI GTAC 3 cut(s) 196, 268, 555
DdeI CTNAG 1 cut(s) 571
DpnI GATC 1 cut(s) 78
DpnII GATC 1 cut(s) 76
EaeI YGGCCR 1 cut(s) 440
Eco130I CCWWGG 1 cut(s) 128
EcoRII CCWGG 1 cut(s) 51
EcoT14I CCWWGG 1 cut(s) 128
EcoT22I ATGCAT 1 cut(s) 108
ErhI CCWWGG 1 cut(s) 128
FaeI CATG 4 cut(s) 242, 333, 499, 545
FalI AAGNNNNNCTT 2 cut(s) 203, 235
FatI CATG 4 cut(s) 238, 329, 495, 541
FauNDI CATATG 1 cut(s) 388
Fnu4HI GCNGC 2 cut(s) 183, 551
FokI GGATG 2 cut(s) 98, 289
Fsp4HI GCNGC 2 cut(s) 183, 551
FspBI CTAG 1 cut(s) 129
GlaI GCGC 1 cut(s) 549
GluI GCNGC 2 cut(s) 183, 551
HaeIII GGCC 1 cut(s) 442
HgaI GACGC 1 cut(s) 51
HhaI GCGC 1 cut(s) 550
Hin1II CATG 4 cut(s) 242, 333, 499, 545
Hin6I GCGC 1 cut(s) 548
HinP1I GCGC 1 cut(s) 548
HinfI GANTC 4 cut(s) 124, 206, 394, 453
HphI GGTGA 2 cut(s) 83, 215
Hpy166II GTNNAC 1 cut(s) 477
Hpy188I TCNGA 1 cut(s) 574
Hpy188III TCNNGA 1 cut(s) 80
Hpy8I GTNNAC 1 cut(s) 477
Hpy99I CGWCG 1 cut(s) 67
HpyAV CCTTC 1 cut(s) 473
HpyCH4V TGCA 1 cut(s) 106
HpyF10VI GCNNNNNNNGC 1 cut(s) 426
HpyF3I CTNAG 1 cut(s) 571
Hsp92II CATG 4 cut(s) 242, 333, 499, 545
HspAI GCGC 1 cut(s) 548
Kzo9I GATC 1 cut(s) 76
LpnPI CCDG 7 cut(s) 9, 38, 65, 102, 171, 434, 477
Lsp1109I GCAGC 2 cut(s) 194, 537
LweI GCATC 3 cut(s) 79, 93, 535
MaeI CTAG 1 cut(s) 129
MaeIII GTNAC 2 cut(s) 311, 391
MalI GATC 1 cut(s) 78
MboI GATC 1 cut(s) 76
MboII GAAGA 2 cut(s) 267, 298
MhlI GDGCHC 1 cut(s) 98
MlsI TGGCCA 1 cut(s) 442
MluCI AATT 1 cut(s) 519
MluNI TGGCCA 1 cut(s) 442
MlyI GAGTC 2 cut(s) 133, 388
MmeI TCCRAC 1 cut(s) 240
MnlI CCTC 6 cut(s) 30, 36, 76, 477, 577, 580
Mox20I TGGCCA 1 cut(s) 442
Mph1103I ATGCAT 1 cut(s) 108
MscI TGGCCA 1 cut(s) 442
MseI TTAA 1 cut(s) 458
MslI CAYNNNNRTG 1 cut(s) 275
Msp20I TGGCCA 1 cut(s) 442
MspR9I CCNGG 1 cut(s) 53
MvaI CCWGG 1 cut(s) 53
MwoI GCNNNNNNNGC 1 cut(s) 426
NdeI CATATG 1 cut(s) 388
NdeII GATC 1 cut(s) 76
NlaIII CATG 4 cut(s) 242, 333, 499, 545
NlaIV GGNNCC 1 cut(s) 406
NmeAIII GCCGAG 1 cut(s) 158
NmuCI GTSAC 1 cut(s) 391
NsiI ATGCAT 1 cut(s) 108
PcsI WCGNNNNNNNCGW 1 cut(s) 119
PfeI GAWTC 2 cut(s) 206, 453
PflMI CCANNNNNTGG 1 cut(s) 26
PkrI GCNGC 2 cut(s) 184, 552
PleI GAGTC 2 cut(s) 132, 388
PpsI GAGTC 2 cut(s) 132, 388
Psp6I CCWGG 1 cut(s) 51
PspGI CCWGG 1 cut(s) 51
PspN4I GGNNCC 1 cut(s) 406
RsaI GTAC 3 cut(s) 197, 269, 556
RsaNI GTAC 3 cut(s) 196, 268, 555
RseI CAYNNNNRTG 1 cut(s) 275
SaqAI TTAA 1 cut(s) 458
SatI GCNGC 2 cut(s) 183, 551
Sau3AI GATC 1 cut(s) 76
SchI GAGTC 2 cut(s) 133, 388
ScrFI CCNGG 1 cut(s) 53
SduI GDGCHC 1 cut(s) 98
SetI ASST 2 cut(s) 113, 121
SfaNI GCATC 3 cut(s) 79, 93, 535
SmiMI CAYNNNNRTG 1 cut(s) 275
SmlI CTYRAG 1 cut(s) 80
SmoI CTYRAG 1 cut(s) 80
Sse9I AATT 1 cut(s) 519
SsiI CCGC 1 cut(s) 155
SspMI CTAG 1 cut(s) 129
StyD4I CCNGG 1 cut(s) 51
StyI CCWWGG 1 cut(s) 128
TaqI TCGA 2 cut(s) 65, 122
TaqII GACCGA 1 cut(s) 261
TasI AATT 1 cut(s) 519
TatI WGTACW 2 cut(s) 195, 267
TfiI GAWTC 2 cut(s) 206, 453
Tru1I TTAA 1 cut(s) 458
Tru9I TTAA 1 cut(s) 458
TseFI GTSAC 1 cut(s) 391
TseI GCWGC 2 cut(s) 182, 550
Tsp45I GTSAC 1 cut(s) 391
TspDTI ATGAA 1 cut(s) 366
Van91I CCANNNNNTGG 1 cut(s) 26
XmaJI CCTAGG 1 cut(s) 128
XspI CTAG 1 cut(s) 129
Zsp2I ATGCAT 1 cut(s) 108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.