Rroxscaffold_2G00152300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0012408
Physical Location & Seq
Reverse (-)
89309317 .. 89312506
3190 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00152300.1

Sequence Viewer

Length: 456 bp
ATGTCGGGTGGCACACCAACCGGTGGATACATGAGGCAGAGGCATAGCCAGGGCTATGCGTCGAGTGGAGATGATCTTGAGGATGATGCATGCTCGATGATGCATAGTCCGTCAGCTTCGAGTCCGAGAGTTTGGTCAAAGAGAGAGATTGCGGAGAATTTGATTTGGCTTGCCTCGGCAGCAGGAATTATGTACTTGGGTGATTCGAAATCCAACTTGATATATATCTTGTGGCATGATGACCGAATAAGAAGATTGCCATTGTACATTGGGATGGTGGGCGTGGTTTTGAATTTTATCATCTTCTGTTACACAAGTATGTCAGCATGGAGTGTCGGGCGATTTGATGAAAAATGGGAATTAGCAAGTATATCTGCTCTGCCGTTTGTGACACTACTTGGTGCCATTTCCTTTTGCCTGTTCTGCTTTGCTCTGTGGCCAATATGGGGGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

151

Amino Acids

16.91

Weight (kDa)

5.84

Isoelectric Point (pI)

57.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM128 PF20479 48 - 151 1e-22 TMEM128 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012408)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20120 AT5G20120
fragaria_vesca FvH4_1g03550
malus_domestica MD02G1034700.v1.1 MD15G1175500.v1.1
prunus_persica Prupe.7G240600_v2.0.a1
pyrus_communis pycom02g02830 pycom15g15700
rosa_chinensis RchiOBHm_Chr2g0089051
rosa_laevigata RLG00000015987
rosa_multiflora Rmu_sc0005510.1_g000007
rosa_roxburghii Rroxscaffold_2G00152300
rosa_rugosa Rorug01G0487000 Rorug01G0487100
rosa_samantha Rh2AG042500 Rh2BG041000 Rh2CG042600 Rh2DG041600
rosa_wichuraiana Rw2G003510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 401
AccB7I CCANNNNNTGG 1 cut(s) 23
AciI CCGC 1 cut(s) 152
AcoI YGGCCR 1 cut(s) 437
AcsI RAATTY 2 cut(s) 157, 292
AfaI GTAC 2 cut(s) 194, 266
AfiI CCNNNNNNNGG 2 cut(s) 23, 446
AgeI ACCGGT 1 cut(s) 20
AgsI TTSAA 1 cut(s) 292
AjnI CCWGG 1 cut(s) 48
AluBI AGCT 1 cut(s) 116
AluI AGCT 1 cut(s) 116
AoxI GGCC 1 cut(s) 437
ApeKI GCWGC 1 cut(s) 179
ApoI RAATTY 2 cut(s) 157, 292
AsiGI ACCGGT 1 cut(s) 20
AsuHPI GGTGA 1 cut(s) 212
AsuII TTCGAA 1 cut(s) 206
BalI TGGCCA 1 cut(s) 439
BanI GGYRCC 1 cut(s) 401
BbvI GCAGC 1 cut(s) 191
BccI CCATC 1 cut(s) 268
BceAI ACGGC 1 cut(s) 367
BciT130I CCWGG 1 cut(s) 50
BciVI GTATCC 1 cut(s) 20
BfuI GTATCC 1 cut(s) 20
BisI GCNGC 1 cut(s) 180
BlsI GCNGC 1 cut(s) 181
Bme1390I CCNGG 1 cut(s) 50
BmiI GGNNCC 1 cut(s) 403
BmrFI CCNGG 1 cut(s) 50
BmsI GCATC 2 cut(s) 76, 90
Bpu14I TTCGAA 1 cut(s) 206
BpuEI CTTGAG 1 cut(s) 98
BsaBI GATNNNNATC 1 cut(s) 224
BsaJI CCNNGG 2 cut(s) 49, 174
BsaWI WCCGGW 1 cut(s) 20
Bsc4I CCNNNNNNNGG 2 cut(s) 23, 446
Bse118I RCCGGY 1 cut(s) 20
Bse8I GATNNNNATC 1 cut(s) 224
BseBI CCWGG 1 cut(s) 50
BseDI CCNNGG 2 cut(s) 49, 174
BseGI GGATG 2 cut(s) 88, 279
BseJI GATNNNNATC 1 cut(s) 224
BseLI CCNNNNNNNGG 2 cut(s) 23, 446
BseXI GCAGC 1 cut(s) 191
BshFI GGCC 1 cut(s) 439
BshNI GGYRCC 1 cut(s) 401
BshTI ACCGGT 1 cut(s) 20
BsiSI CCGG 1 cut(s) 21
BslI CCNNNNNNNGG 2 cut(s) 23, 446
BsnI GGCC 1 cut(s) 439
Bsp119I TTCGAA 1 cut(s) 206
Bsp1407I TGTACA 1 cut(s) 264
Bsp143I GATC 1 cut(s) 73
BspACI CCGC 1 cut(s) 152
BspANI GGCC 1 cut(s) 439
BspLI GGNNCC 1 cut(s) 403
BspT104I TTCGAA 1 cut(s) 206
BspT107I GGYRCC 1 cut(s) 401
BsrFI RCCGGY 1 cut(s) 20
BsrGI TGTACA 1 cut(s) 264
BssAI RCCGGY 1 cut(s) 20
BssECI CCNNGG 2 cut(s) 49, 174
BssMI GATC 1 cut(s) 73
Bst2UI CCWGG 1 cut(s) 50
BstAUI TGTACA 1 cut(s) 264
BstBI TTCGAA 1 cut(s) 206
BstC8I GCNNGC 2 cut(s) 91, 171
BstF5I GGATG 2 cut(s) 88, 279
BstKTI GATC 1 cut(s) 76
BstMBI GATC 1 cut(s) 73
BstMWI GCNNNNNNNGC 2 cut(s) 179, 423
BstNI CCWGG 1 cut(s) 50
BstNSI RCATGY 1 cut(s) 93
BstSCI CCNGG 1 cut(s) 48
BstV1I GCAGC 1 cut(s) 191
BsuI GTATCC 1 cut(s) 20
BsuRI GGCC 1 cut(s) 439
BtsCI GGATG 2 cut(s) 88, 279
Cac8I GCNNGC 2 cut(s) 91, 171
Cfr10I RCCGGY 1 cut(s) 20
CseI GACGC 1 cut(s) 48
Csp6I GTAC 2 cut(s) 193, 265
CspAI ACCGGT 1 cut(s) 20
CviAII CATG 4 cut(s) 31, 90, 236, 327
CviJI RGCY 5 cut(s) 48, 54, 116, 169, 439
CviKI_1 RGCY 5 cut(s) 48, 54, 116, 169, 439
CviQI GTAC 2 cut(s) 193, 265
DpnI GATC 1 cut(s) 75
DpnII GATC 1 cut(s) 73
EaeI YGGCCR 1 cut(s) 437
EcoRII CCWGG 1 cut(s) 48
EcoT22I ATGCAT 2 cut(s) 91, 105
FaeI CATG 4 cut(s) 34, 93, 239, 330
FatI CATG 4 cut(s) 30, 89, 235, 326
Fnu4HI GCNGC 1 cut(s) 180
FokI GGATG 2 cut(s) 95, 286
Fsp4HI GCNGC 1 cut(s) 180
GluI GCNGC 1 cut(s) 180
HaeIII GGCC 1 cut(s) 439
HapII CCGG 1 cut(s) 21
HgaI GACGC 1 cut(s) 48
Hin1II CATG 4 cut(s) 34, 93, 239, 330
HinfI GANTC 2 cut(s) 121, 203
HpaII CCGG 1 cut(s) 21
HphI GGTGA 1 cut(s) 212
Hpy188I TCNGA 1 cut(s) 126
Hpy188III TCNNGA 1 cut(s) 77
Hpy99I CGWCG 1 cut(s) 64
HpyCH4V TGCA 2 cut(s) 89, 103
HpyF10VI GCNNNNNNNGC 2 cut(s) 179, 423
Hsp92II CATG 4 cut(s) 34, 93, 239, 330
Kzo9I GATC 1 cut(s) 73
LpnPI CCDG 5 cut(s) 34, 35, 62, 168, 431
Lsp1109I GCAGC 1 cut(s) 191
LweI GCATC 2 cut(s) 76, 90
MaeIII GTNAC 2 cut(s) 308, 388
MalI GATC 1 cut(s) 75
MboI GATC 1 cut(s) 73
MboII GAAGA 2 cut(s) 264, 295
MlsI TGGCCA 1 cut(s) 439
MluCI AATT 4 cut(s) 157, 186, 292, 359
MluNI TGGCCA 1 cut(s) 439
MlyI GAGTC 1 cut(s) 130
MmeI TCCRAC 1 cut(s) 237
MnlI CCTC 4 cut(s) 27, 33, 73, 184
Mox20I TGGCCA 1 cut(s) 439
Mph1103I ATGCAT 2 cut(s) 91, 105
MscI TGGCCA 1 cut(s) 439
MslI CAYNNNNRTG 2 cut(s) 272, 317
Msp20I TGGCCA 1 cut(s) 439
MspI CCGG 1 cut(s) 21
MspR9I CCNGG 1 cut(s) 50
MvaI CCWGG 1 cut(s) 50
MwoI GCNNNNNNNGC 2 cut(s) 179, 423
NdeII GATC 1 cut(s) 73
NlaIII CATG 4 cut(s) 34, 93, 239, 330
NlaIV GGNNCC 1 cut(s) 403
NmeAIII GCCGAG 1 cut(s) 155
NmuCI GTSAC 1 cut(s) 388
NsiI ATGCAT 2 cut(s) 91, 105
NspI RCATGY 1 cut(s) 93
NspV TTCGAA 1 cut(s) 206
PaeI GCATGC 1 cut(s) 93
PfeI GAWTC 1 cut(s) 203
PflMI CCANNNNNTGG 1 cut(s) 23
PinAI ACCGGT 1 cut(s) 20
PkrI GCNGC 1 cut(s) 181
PleI GAGTC 1 cut(s) 129
PpsI GAGTC 1 cut(s) 129
Psp6I CCWGG 1 cut(s) 48
PspGI CCWGG 1 cut(s) 48
PspN4I GGNNCC 1 cut(s) 403
RsaI GTAC 2 cut(s) 194, 266
RsaNI GTAC 2 cut(s) 193, 265
RseI CAYNNNNRTG 2 cut(s) 272, 317
SatI GCNGC 1 cut(s) 180
Sau3AI GATC 1 cut(s) 73
SchI GAGTC 1 cut(s) 130
ScrFI CCNGG 1 cut(s) 50
SetI ASST 1 cut(s) 118
SfaNI GCATC 2 cut(s) 76, 90
SfuI TTCGAA 1 cut(s) 206
SmiMI CAYNNNNRTG 2 cut(s) 272, 317
SmlI CTYRAG 1 cut(s) 77
SmoI CTYRAG 1 cut(s) 77
SphI GCATGC 1 cut(s) 93
Sse9I AATT 4 cut(s) 157, 186, 292, 359
SsiI CCGC 1 cut(s) 152
StyD4I CCNGG 1 cut(s) 48
TaqI TCGA 4 cut(s) 62, 95, 119, 206
TaqII GACCGA 1 cut(s) 258
TasI AATT 4 cut(s) 157, 186, 292, 359
TatI WGTACW 2 cut(s) 192, 264
TfiI GAWTC 1 cut(s) 203
TseFI GTSAC 1 cut(s) 388
TseI GCWGC 1 cut(s) 179
Tsp45I GTSAC 1 cut(s) 388
TspDTI ATGAA 1 cut(s) 363
TspGWI ACGGA 1 cut(s) 99
Van91I CCANNNNNTGG 1 cut(s) 23
XapI RAATTY 2 cut(s) 157, 292
XceI RCATGY 1 cut(s) 93
Zsp2I ATGCAT 2 cut(s) 91, 105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.