MD15G1175500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0012408
Physical Location & Seq
Reverse (-)
13639465 .. 13641879
2415 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1175500.v1.1.491

Sequence Viewer

Length: 594 bp
ATGTCGGGTGGTACGCCAACTGGCGCCAGATACATGAGGCAGAGACATAGCCAGGGCTATGCATCTAGCGGCGATGATCTTGAGGATGATGCATGCTCGGTTATGCGCACTTCATCCCCACAAAGTCCGAAAGTTTGGTCACGGATTGAGATTTTTGAGAATGTCCTTTGGCTTGCCTCAGCAGCATTCATTGTGTACTATGGTGACAGGAAATCCAACTTGATATATCTCTTCTGCCATGATGAGAGAATTAGAAGATTGCCTTTATACCTTGGGATGGCGGGCATAGTTTTAAACATTATCATCTTCTTTTATACGAGTATGTCGGCATGGAGCGTGAGGAGATTTGACGAGAAATGGGAATTAGCAAGTATATCTGCTTTGCCGTTTGTGACACTACTTGGCGGCATCTCCTTTTGCTTCTTCTGCTTCGCTTTGTGGCCAATATGGAGTTTCTTGACGCTTCCTCTTCTGTTCACGCTGTTTATGGCAGGCATGGTTATATGCCCATACATTATAATTGGGATATTTAGGCAACAGCATGACGTGCTGCGTACAGATTATGCCTCGGAGTTAGGTGAAAAACAATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

198

Amino Acids

22.64

Weight (kDa)

6.81

Isoelectric Point (pI)

58.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM128 PF20479 48 - 171 2.8e-30 TMEM128 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012408)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20120 AT5G20120
fragaria_vesca FvH4_1g03550
malus_domestica MD02G1034700.v1.1 MD15G1175500.v1.1
prunus_persica Prupe.7G240600_v2.0.a1
pyrus_communis pycom02g02830 pycom15g15700
rosa_chinensis RchiOBHm_Chr2g0089051
rosa_laevigata RLG00000015987
rosa_multiflora Rmu_sc0005510.1_g000007
rosa_roxburghii Rroxscaffold_2G00152300
rosa_rugosa Rorug01G0487000 Rorug01G0487100
rosa_samantha Rh2AG042500 Rh2BG041000 Rh2CG042600 Rh2DG041600
rosa_wichuraiana Rw2G003510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 518
Acc16I TGCGCA 1 cut(s) 107
AccB1I GGYRCC 1 cut(s) 23
AciI CCGC 3 cut(s) 69, 281, 405
AcoI YGGCCR 1 cut(s) 440
AcyI GRCGYC 1 cut(s) 24
AfaI GTAC 3 cut(s) 13, 197, 556
AfiI CCNNNNNNNGG 1 cut(s) 277
AjiI CACGTC 1 cut(s) 547
AjnI CCWGG 1 cut(s) 51
Alw26I GTCTC 1 cut(s) 37
AoxI GGCC 1 cut(s) 440
ApeKI GCWGC 2 cut(s) 182, 550
ArsI GACNNNNNNTTYG 2 cut(s) 122, 154
AspLEI GCGC 2 cut(s) 26, 108
AsuHPI GGTGA 2 cut(s) 215, 590
BalI TGGCCA 1 cut(s) 442
BanI GGYRCC 1 cut(s) 23
BbvCI CCTCAGC 1 cut(s) 178
BbvI GCAGC 2 cut(s) 194, 537
BccI CCATC 1 cut(s) 271
BceAI ACGGC 1 cut(s) 370
BciT130I CCWGG 1 cut(s) 53
BcoDI GTCTC 1 cut(s) 37
BfaI CTAG 1 cut(s) 66
BfoI RGCGCY 1 cut(s) 27
BisI GCNGC 4 cut(s) 70, 183, 406, 551
BlsI GCNGC 4 cut(s) 71, 184, 407, 552
Bme1390I CCNGG 1 cut(s) 53
BmgBI CACGTC 1 cut(s) 547
BmiI GGNNCC 1 cut(s) 25
BmrFI CCNGG 1 cut(s) 53
BmsI GCATC 3 cut(s) 71, 79, 417
Bpu10I CCTNAGC 1 cut(s) 178
BpuEI CTTGAG 1 cut(s) 101
BsaHI GRCGYC 1 cut(s) 24
BsaJI CCNNGG 3 cut(s) 52, 271, 567
Bsc4I CCNNNNNNNGG 1 cut(s) 277
Bse1I ACTGG 1 cut(s) 25
BseBI CCWGG 1 cut(s) 53
BseDI CCNNGG 3 cut(s) 52, 271, 567
BseGI GGATG 3 cut(s) 91, 113, 282
BseLI CCNNNNNNNGG 1 cut(s) 277
BseMII CTCAG 1 cut(s) 192
BseNI ACTGG 1 cut(s) 25
BseRI GAGGAG 1 cut(s) 355
BseXI GCAGC 2 cut(s) 194, 537
BshFI GGCC 1 cut(s) 442
BshNI GGYRCC 1 cut(s) 23
BslI CCNNNNNNNGG 1 cut(s) 277
BsmAI GTCTC 1 cut(s) 37
BsmI GAATGC 1 cut(s) 185
BsnI GGCC 1 cut(s) 442
Bsp143I GATC 1 cut(s) 76
BspACI CCGC 3 cut(s) 69, 281, 405
BspANI GGCC 1 cut(s) 442
BspCNI CTCAG 1 cut(s) 191
BspLI GGNNCC 1 cut(s) 25
BspT107I GGYRCC 1 cut(s) 23
BsrI ACTGG 1 cut(s) 25
BssECI CCNNGG 3 cut(s) 52, 271, 567
BssMI GATC 1 cut(s) 76
BssNI GRCGYC 1 cut(s) 24
BssT1I CCWWGG 1 cut(s) 271
Bst2UI CCWGG 1 cut(s) 53
Bst6I CTCTTC 2 cut(s) 236, 474
BstACI GRCGYC 1 cut(s) 24
BstAPI GCANNNNNTGC 1 cut(s) 547
BstC8I GCNNGC 4 cut(s) 94, 174, 283, 493
BstDEI CTNAG 1 cut(s) 178
BstF5I GGATG 3 cut(s) 91, 113, 282
BstH2I RGCGCY 1 cut(s) 27
BstHHI GCGC 2 cut(s) 26, 108
BstKTI GATC 1 cut(s) 79
BstMAI GTCTC 1 cut(s) 37
BstMBI GATC 1 cut(s) 76
BstMWI GCNNNNNNNGC 3 cut(s) 182, 426, 547
BstNI CCWGG 1 cut(s) 53
BstNSI RCATGY 1 cut(s) 96
BstSCI CCNGG 1 cut(s) 51
BstV1I GCAGC 2 cut(s) 194, 537
BsuRI GGCC 1 cut(s) 442
BtgZI GCGATG 1 cut(s) 87
BtrI CACGTC 1 cut(s) 547
BtsCI GGATG 3 cut(s) 91, 113, 282
Cac8I GCNNGC 4 cut(s) 94, 174, 283, 493
CfoI GCGC 2 cut(s) 26, 108
CseI GACGC 1 cut(s) 469
Csp6I GTAC 3 cut(s) 12, 196, 555
CviAII CATG 6 cut(s) 34, 93, 239, 330, 496, 542
CviJI RGCY 4 cut(s) 51, 57, 172, 442
CviKI_1 RGCY 4 cut(s) 51, 57, 172, 442
CviQI GTAC 3 cut(s) 12, 196, 555
DdeI CTNAG 1 cut(s) 178
DinI GGCGCC 1 cut(s) 25
DpnI GATC 1 cut(s) 78
DpnII GATC 1 cut(s) 76
DraI TTTAAA 1 cut(s) 294
EaeI YGGCCR 1 cut(s) 440
Eam1104I CTCTTC 2 cut(s) 236, 474
EarI CTCTTC 2 cut(s) 236, 474
Eco130I CCWWGG 1 cut(s) 271
EcoRII CCWGG 1 cut(s) 51
EcoT14I CCWWGG 1 cut(s) 271
EcoT22I ATGCAT 2 cut(s) 64, 94
EgeI GGCGCC 1 cut(s) 25
EheI GGCGCC 1 cut(s) 25
ErhI CCWWGG 1 cut(s) 271
FaeI CATG 6 cut(s) 37, 96, 242, 333, 499, 545
FalI AAGNNNNNCTT 2 cut(s) 247, 279
FatI CATG 6 cut(s) 33, 92, 238, 329, 495, 541
FauI CCCGC 1 cut(s) 274
Fnu4HI GCNGC 4 cut(s) 70, 183, 406, 551
FokI GGATG 3 cut(s) 98, 100, 289
Fsp4HI GCNGC 4 cut(s) 70, 183, 406, 551
FspAI RTGCGCAY 1 cut(s) 107
FspBI CTAG 1 cut(s) 66
FspI TGCGCA 1 cut(s) 107
GlaI GCGC 2 cut(s) 25, 107
GluI GCNGC 4 cut(s) 70, 183, 406, 551
HaeII RGCGCY 1 cut(s) 27
HaeIII GGCC 1 cut(s) 442
HgaI GACGC 1 cut(s) 469
HhaI GCGC 2 cut(s) 26, 108
Hin1I GRCGYC 1 cut(s) 24
Hin1II CATG 6 cut(s) 37, 96, 242, 333, 499, 545
Hin6I GCGC 2 cut(s) 24, 106
HinP1I GCGC 2 cut(s) 24, 106
HphI GGTGA 2 cut(s) 215, 590
Hpy166II GTNNAC 2 cut(s) 196, 477
Hpy188I TCNGA 2 cut(s) 129, 571
Hpy188III TCNNGA 2 cut(s) 80, 457
Hpy8I GTNNAC 2 cut(s) 196, 477
HpyCH4IV ACGT 1 cut(s) 546
HpyCH4V TGCA 2 cut(s) 62, 92
HpyF10VI GCNNNNNNNGC 3 cut(s) 182, 426, 547
HpyF3I CTNAG 1 cut(s) 178
HpySE526I ACGT 1 cut(s) 546
Hsp92I GRCGYC 1 cut(s) 24
Hsp92II CATG 6 cut(s) 37, 96, 242, 333, 499, 545
HspAI GCGC 2 cut(s) 24, 106
KasI GGCGCC 1 cut(s) 23
Kzo9I GATC 1 cut(s) 76
LmnI GCTCC 1 cut(s) 333
LpnPI CCDG 6 cut(s) 6, 38, 40, 65, 193, 477
Lsp1109I GCAGC 2 cut(s) 194, 537
LweI GCATC 3 cut(s) 71, 79, 417
MaeI CTAG 1 cut(s) 66
MaeII ACGT 1 cut(s) 546
MaeIII GTNAC 3 cut(s) 138, 203, 391
MalI GATC 1 cut(s) 78
MboI GATC 1 cut(s) 76
MboII GAAGA 5 cut(s) 223, 267, 298, 415, 461
MlsI TGGCCA 1 cut(s) 442
MluCI AATT 3 cut(s) 249, 362, 519
MluNI TGGCCA 1 cut(s) 442
Mly113I GGCGCC 1 cut(s) 24
MmeI TCCRAC 1 cut(s) 240
MnlI CCTC 6 cut(s) 30, 76, 187, 333, 477, 577
Mox20I TGGCCA 1 cut(s) 442
Mph1103I ATGCAT 2 cut(s) 64, 94
MscI TGGCCA 1 cut(s) 442
MseI TTAA 1 cut(s) 293
Msp20I TGGCCA 1 cut(s) 442
MspR9I CCNGG 1 cut(s) 53
Mva1269I GAATGC 1 cut(s) 185
MvaI CCWGG 1 cut(s) 53
MwoI GCNNNNNNNGC 3 cut(s) 182, 426, 547
NarI GGCGCC 1 cut(s) 24
NdeII GATC 1 cut(s) 76
NlaIII CATG 6 cut(s) 37, 96, 242, 333, 499, 545
NlaIV GGNNCC 1 cut(s) 25
NmuCI GTSAC 3 cut(s) 138, 203, 391
NsbI TGCGCA 1 cut(s) 107
NsiI ATGCAT 2 cut(s) 64, 94
NspI RCATGY 1 cut(s) 96
PaeI GCATGC 1 cut(s) 96
PctI GAATGC 1 cut(s) 185
PkrI GCNGC 4 cut(s) 71, 184, 407, 552
PluTI GGCGCC 1 cut(s) 27
PsiI TTATAA 1 cut(s) 518
Psp6I CCWGG 1 cut(s) 51
PspGI CCWGG 1 cut(s) 51
PspN4I GGNNCC 1 cut(s) 25
RsaI GTAC 3 cut(s) 13, 197, 556
RsaNI GTAC 3 cut(s) 12, 196, 555
SaqAI TTAA 1 cut(s) 293
SatI GCNGC 4 cut(s) 70, 183, 406, 551
Sau3AI GATC 1 cut(s) 76
ScrFI CCNGG 1 cut(s) 53
SetI ASST 3 cut(s) 273, 549, 580
SfaNI GCATC 3 cut(s) 71, 79, 417
SfoI GGCGCC 1 cut(s) 25
SmlI CTYRAG 1 cut(s) 80
SmoI CTYRAG 1 cut(s) 80
SphI GCATGC 1 cut(s) 96
Sse9I AATT 3 cut(s) 249, 362, 519
SsiI CCGC 3 cut(s) 69, 281, 405
SspDI GGCGCC 1 cut(s) 23
SspMI CTAG 1 cut(s) 66
StyD4I CCNGG 1 cut(s) 51
StyI CCWWGG 1 cut(s) 271
TaiI ACGT 1 cut(s) 549
TasI AATT 3 cut(s) 249, 362, 519
TatI WGTACW 1 cut(s) 195
TauI GCSGC 2 cut(s) 72, 408
Tru1I TTAA 1 cut(s) 293
Tru9I TTAA 1 cut(s) 293
TseFI GTSAC 3 cut(s) 138, 203, 391
TseI GCWGC 2 cut(s) 182, 550
Tsp45I GTSAC 3 cut(s) 138, 203, 391
TspDTI ATGAA 2 cut(s) 102, 178
TspGWI ACGGA 1 cut(s) 157
XceI RCATGY 1 cut(s) 96
XspI CTAG 1 cut(s) 66
Zsp2I ATGCAT 2 cut(s) 64, 94
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.