RLG00000015987

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0012408
Physical Location & Seq
Forward (+)
3014838 .. 3016146
1309 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015987

Sequence Viewer

Length: 594 bp
ATGTCGGGTGGCACACCAACCGGTGGATATATGAGGCAGAGGCATAGCCAGGGCTATGCGTCGAGTGGAGATGATCTTGAGGATGATGCATGCTCGATGATGCATAGTCCGTCAGCTTCGAGTTCGAGAGTTTGGTCAAAGAGAGAGATTGCAGAGAATTTGATTTGGCTTGCCTCGGCAGCAGGCATTATGTACTTGGGTGATTCGAAATCCAACTTGATATATATCTTGTGGCATGATGACCGAATAAGAAGATTGCCGTTGTACATTGGGATGGTGGGCGTGGTTTTGAATTTTATCATCTTCTGTTACACAAGTATGTCAGCCTGGAGTGTCGGGCGATTTGATGAAAAATGGGAATTAGCAAGTATATCTGCTCTGCCGTTTGTGACACTACTTGGTGCTATTTCCTTTTGCCTGTTCTGCTTTGCTCTGTGGCCAATATGGGGGTTCTTGACCCTTCCTCTTCTGTTCACGCTGTTTATGGCAGGCATGGTTATATTTCCATACATTATAATTGGGATATTTAGGAAACAGCATGATCTGCTGCGCACGGATAATGCCTTCTCAGATTTTAGTGAGAAACAATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

198

Amino Acids

22.3

Weight (kDa)

6.05

Isoelectric Point (pI)

51.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM128 PF20479 48 - 171 5.2e-30 TMEM128 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012408)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20120 AT5G20120
fragaria_vesca FvH4_1g03550
malus_domestica MD02G1034700.v1.1 MD15G1175500.v1.1
prunus_persica Prupe.7G240600_v2.0.a1
pyrus_communis pycom02g02830 pycom15g15700
rosa_chinensis RchiOBHm_Chr2g0089051
rosa_laevigata RLG00000015987
rosa_multiflora Rmu_sc0005510.1_g000007
rosa_roxburghii Rroxscaffold_2G00152300
rosa_rugosa Rorug01G0487000 Rorug01G0487100
rosa_samantha Rh2AG042500 Rh2BG041000 Rh2CG042600 Rh2DG041600
rosa_wichuraiana Rw2G003510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 515
Acc16I TGCGCA 1 cut(s) 551
AccB7I CCANNNNNTGG 1 cut(s) 23
AcoI YGGCCR 1 cut(s) 437
AcsI RAATTY 2 cut(s) 157, 292
AfaI GTAC 2 cut(s) 194, 266
AfiI CCNNNNNNNGG 2 cut(s) 23, 446
AgeI ACCGGT 1 cut(s) 20
AgsI TTSAA 1 cut(s) 292
AjnI CCWGG 2 cut(s) 48, 326
AluBI AGCT 1 cut(s) 116
AluI AGCT 1 cut(s) 116
AoxI GGCC 1 cut(s) 437
ApeKI GCWGC 2 cut(s) 179, 547
ApoI RAATTY 2 cut(s) 157, 292
AsiGI ACCGGT 1 cut(s) 20
AspLEI GCGC 1 cut(s) 552
AsuHPI GGTGA 1 cut(s) 212
AsuII TTCGAA 1 cut(s) 206
BalI TGGCCA 1 cut(s) 439
BbvI GCAGC 2 cut(s) 191, 534
BccI CCATC 1 cut(s) 268
BceAI ACGGC 2 cut(s) 244, 367
BciT130I CCWGG 2 cut(s) 50, 328
BisI GCNGC 2 cut(s) 180, 548
BlsI GCNGC 2 cut(s) 181, 549
Bme1390I CCNGG 2 cut(s) 50, 328
BmrFI CCNGG 2 cut(s) 50, 328
BmsI GCATC 2 cut(s) 76, 90
BpmI CTGGAG 1 cut(s) 349
Bpu14I TTCGAA 1 cut(s) 206
BpuEI CTTGAG 1 cut(s) 98
BsaBI GATNNNNATC 1 cut(s) 224
BsaJI CCNNGG 2 cut(s) 49, 174
BsaWI WCCGGW 1 cut(s) 20
Bsc4I CCNNNNNNNGG 2 cut(s) 23, 446
Bse118I RCCGGY 1 cut(s) 20
Bse8I GATNNNNATC 1 cut(s) 224
BseBI CCWGG 2 cut(s) 50, 328
BseDI CCNNGG 2 cut(s) 49, 174
BseGI GGATG 2 cut(s) 88, 279
BseJI GATNNNNATC 1 cut(s) 224
BseLI CCNNNNNNNGG 2 cut(s) 23, 446
BseMII CTCAG 1 cut(s) 582
BseXI GCAGC 2 cut(s) 191, 534
BshFI GGCC 1 cut(s) 439
BshTI ACCGGT 1 cut(s) 20
BsiSI CCGG 1 cut(s) 21
BslI CCNNNNNNNGG 2 cut(s) 23, 446
BsnI GGCC 1 cut(s) 439
Bsp119I TTCGAA 1 cut(s) 206
Bsp1407I TGTACA 1 cut(s) 264
Bsp143I GATC 2 cut(s) 73, 541
BspANI GGCC 1 cut(s) 439
BspCNI CTCAG 1 cut(s) 581
BspT104I TTCGAA 1 cut(s) 206
BsrFI RCCGGY 1 cut(s) 20
BsrGI TGTACA 1 cut(s) 264
BssAI RCCGGY 1 cut(s) 20
BssECI CCNNGG 2 cut(s) 49, 174
BssMI GATC 2 cut(s) 73, 541
Bst2UI CCWGG 2 cut(s) 50, 328
Bst6I CTCTTC 1 cut(s) 471
BstAPI GCANNNNNTGC 1 cut(s) 544
BstAUI TGTACA 1 cut(s) 264
BstBI TTCGAA 1 cut(s) 206
BstC8I GCNNGC 4 cut(s) 91, 171, 184, 490
BstDEI CTNAG 1 cut(s) 568
BstF5I GGATG 2 cut(s) 88, 279
BstHHI GCGC 1 cut(s) 552
BstKTI GATC 2 cut(s) 76, 544
BstMBI GATC 2 cut(s) 73, 541
BstMWI GCNNNNNNNGC 3 cut(s) 179, 423, 544
BstNI CCWGG 2 cut(s) 50, 328
BstNSI RCATGY 1 cut(s) 93
BstSCI CCNGG 2 cut(s) 48, 326
BstV1I GCAGC 2 cut(s) 191, 534
BsuRI GGCC 1 cut(s) 439
BtsCI GGATG 2 cut(s) 88, 279
Cac8I GCNNGC 4 cut(s) 91, 171, 184, 490
CfoI GCGC 1 cut(s) 552
Cfr10I RCCGGY 1 cut(s) 20
CseI GACGC 1 cut(s) 48
Csp6I GTAC 2 cut(s) 193, 265
CspAI ACCGGT 1 cut(s) 20
CviAII CATG 4 cut(s) 90, 236, 493, 539
CviJI RGCY 6 cut(s) 48, 54, 116, 169, 326, 439
CviKI_1 RGCY 6 cut(s) 48, 54, 116, 169, 326, 439
CviQI GTAC 2 cut(s) 193, 265
DdeI CTNAG 1 cut(s) 568
DpnI GATC 2 cut(s) 75, 543
DpnII GATC 2 cut(s) 73, 541
EaeI YGGCCR 1 cut(s) 437
Eam1104I CTCTTC 1 cut(s) 471
EarI CTCTTC 1 cut(s) 471
EcoRII CCWGG 2 cut(s) 48, 326
EcoT22I ATGCAT 2 cut(s) 91, 105
FaeI CATG 4 cut(s) 93, 239, 496, 542
FatI CATG 4 cut(s) 89, 235, 492, 538
Fnu4HI GCNGC 2 cut(s) 180, 548
FokI GGATG 2 cut(s) 95, 286
Fsp4HI GCNGC 2 cut(s) 180, 548
FspI TGCGCA 1 cut(s) 551
GlaI GCGC 1 cut(s) 551
GluI GCNGC 2 cut(s) 180, 548
GsuI CTGGAG 1 cut(s) 349
HaeIII GGCC 1 cut(s) 439
HapII CCGG 1 cut(s) 21
HgaI GACGC 1 cut(s) 48
HhaI GCGC 1 cut(s) 552
Hin1II CATG 4 cut(s) 93, 239, 496, 542
Hin6I GCGC 1 cut(s) 550
HinP1I GCGC 1 cut(s) 550
HinfI GANTC 1 cut(s) 203
HpaII CCGG 1 cut(s) 21
HphI GGTGA 1 cut(s) 212
Hpy166II GTNNAC 1 cut(s) 474
Hpy188I TCNGA 1 cut(s) 571
Hpy188III TCNNGA 3 cut(s) 77, 126, 454
Hpy8I GTNNAC 1 cut(s) 474
Hpy99I CGWCG 1 cut(s) 64
HpyAV CCTTC 2 cut(s) 470, 574
HpyCH4V TGCA 3 cut(s) 89, 103, 152
HpyF10VI GCNNNNNNNGC 3 cut(s) 179, 423, 544
HpyF3I CTNAG 1 cut(s) 568
Hsp92II CATG 4 cut(s) 93, 239, 496, 542
HspAI GCGC 1 cut(s) 550
Kzo9I GATC 2 cut(s) 73, 541
LpnPI CCDG 8 cut(s) 34, 35, 62, 168, 313, 340, 431, 474
Lsp1109I GCAGC 2 cut(s) 191, 534
LweI GCATC 2 cut(s) 76, 90
MaeIII GTNAC 2 cut(s) 308, 388
MalI GATC 2 cut(s) 75, 543
MboI GATC 2 cut(s) 73, 541
MboII GAAGA 3 cut(s) 264, 295, 458
MlsI TGGCCA 1 cut(s) 439
MluCI AATT 4 cut(s) 157, 292, 359, 516
MluNI TGGCCA 1 cut(s) 439
MmeI TCCRAC 1 cut(s) 237
MnlI CCTC 5 cut(s) 27, 33, 73, 184, 474
Mox20I TGGCCA 1 cut(s) 439
Mph1103I ATGCAT 2 cut(s) 91, 105
MscI TGGCCA 1 cut(s) 439
MslI CAYNNNNRTG 2 cut(s) 272, 317
Msp20I TGGCCA 1 cut(s) 439
MspI CCGG 1 cut(s) 21
MspR9I CCNGG 2 cut(s) 50, 328
MvaI CCWGG 2 cut(s) 50, 328
MwoI GCNNNNNNNGC 3 cut(s) 179, 423, 544
NdeII GATC 2 cut(s) 73, 541
NlaIII CATG 4 cut(s) 93, 239, 496, 542
NmeAIII GCCGAG 1 cut(s) 155
NmuCI GTSAC 1 cut(s) 388
NsbI TGCGCA 1 cut(s) 551
NsiI ATGCAT 2 cut(s) 91, 105
NspI RCATGY 1 cut(s) 93
NspV TTCGAA 1 cut(s) 206
PaeI GCATGC 1 cut(s) 93
PfeI GAWTC 1 cut(s) 203
PflMI CCANNNNNTGG 1 cut(s) 23
PinAI ACCGGT 1 cut(s) 20
PkrI GCNGC 2 cut(s) 181, 549
PsiI TTATAA 1 cut(s) 515
Psp6I CCWGG 2 cut(s) 48, 326
PspGI CCWGG 2 cut(s) 48, 326
RsaI GTAC 2 cut(s) 194, 266
RsaNI GTAC 2 cut(s) 193, 265
RseI CAYNNNNRTG 2 cut(s) 272, 317
SatI GCNGC 2 cut(s) 180, 548
Sau3AI GATC 2 cut(s) 73, 541
ScrFI CCNGG 2 cut(s) 50, 328
SetI ASST 1 cut(s) 118
SfaNI GCATC 2 cut(s) 76, 90
SfuI TTCGAA 1 cut(s) 206
SmiMI CAYNNNNRTG 2 cut(s) 272, 317
SmlI CTYRAG 1 cut(s) 77
SmoI CTYRAG 1 cut(s) 77
SphI GCATGC 1 cut(s) 93
Sse9I AATT 4 cut(s) 157, 292, 359, 516
StyD4I CCNGG 2 cut(s) 48, 326
TaqI TCGA 5 cut(s) 62, 95, 119, 125, 206
TaqII GACCGA 1 cut(s) 258
TasI AATT 4 cut(s) 157, 292, 359, 516
TatI WGTACW 2 cut(s) 192, 264
TfiI GAWTC 1 cut(s) 203
TseFI GTSAC 1 cut(s) 388
TseI GCWGC 2 cut(s) 179, 547
Tsp45I GTSAC 1 cut(s) 388
TspDTI ATGAA 1 cut(s) 363
TspGWI ACGGA 2 cut(s) 99, 569
Van91I CCANNNNNTGG 1 cut(s) 23
XapI RAATTY 2 cut(s) 157, 292
XceI RCATGY 1 cut(s) 93
Zsp2I ATGCAT 2 cut(s) 91, 105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.