pycom02g02830

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0012408
Physical Location & Seq
Reverse (-)
1793436 .. 1794814
1379 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g02830.4

Sequence Viewer

Length: 594 bp
ATGTCGGGTGGTACGCCAACTGGGGCCGGATATATGAGGCAGAGGCATAGCCAGGGATATGCATCGAGCGGCGATGATCTTGAGGATGATGCATGCTCGGTTATGCGCTCTCCGTCCCCACAAAGTCCGAAAGTTTGGTCGCGGATTGAGATTTTGGAGAATGCCCTTTGGCTTGCCTCAGCAGCATTCATTGTGTACTATGGTGACAGGAAATCCAATATGATATATATCTTCTGCCATGATGAGCGAATTAGAAGATTGCCTTTATACCTTGGGATGGTGGGCGTAGTTTTAAACATTATCATCTTCTTTTATACGAGTATGTCGGCATGGAGCGTAAGGAGATTTGACGAGAAATGGGAATTAGAAAGTATAACTGCTCTGCCGTTTGTGACACTACTTGGCGGCATCTCATTTTGCTTCTTCTGCTTTGCTTTGTGGCCAATATGGAGTTTCTTGACGCTTCCTCTTTTGTTCACGCTGTTTATGGCAGGCATGGTTATATGTCCGTACATTATAATCGGGATATTTAGGCAACAGCATGATGTGCTGCGTACAGATTATGCCTCAGAGTTAGGTGAGAAACATTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

198

Amino Acids

22.59

Weight (kDa)

6.06

Isoelectric Point (pI)

66.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012408)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20120 AT5G20120
fragaria_vesca FvH4_1g03550
malus_domestica MD02G1034700.v1.1 MD15G1175500.v1.1
prunus_persica Prupe.7G240600_v2.0.a1
pyrus_communis pycom02g02830 pycom15g15700
rosa_chinensis RchiOBHm_Chr2g0089051
rosa_laevigata RLG00000015987
rosa_multiflora Rmu_sc0005510.1_g000007
rosa_roxburghii Rroxscaffold_2G00152300
rosa_rugosa Rorug01G0487000 Rorug01G0487100
rosa_samantha Rh2AG042500 Rh2BG041000 Rh2CG042600 Rh2DG041600
rosa_wichuraiana Rw2G003510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 518
AccBSI CCGCTC 1 cut(s) 69
AccII CGCG 1 cut(s) 142
AciI CCGC 3 cut(s) 69, 142, 405
AcoI YGGCCR 1 cut(s) 440
AfaI GTAC 4 cut(s) 13, 197, 512, 556
AfiI CCNNNNNNNGG 1 cut(s) 277
AjnI CCWGG 1 cut(s) 51
AoxI GGCC 2 cut(s) 24, 440
ApeKI GCWGC 2 cut(s) 182, 550
ArsI GACNNNNNNTTYG 2 cut(s) 122, 154
AspLEI GCGC 1 cut(s) 108
AspS9I GGNCC 1 cut(s) 24
AsuHPI GGTGA 2 cut(s) 215, 590
BalI TGGCCA 1 cut(s) 442
BbvCI CCTCAGC 1 cut(s) 178
BbvI GCAGC 2 cut(s) 194, 537
BccI CCATC 1 cut(s) 271
BceAI ACGGC 1 cut(s) 370
BciT130I CCWGG 1 cut(s) 53
BisI GCNGC 4 cut(s) 70, 183, 406, 551
BlsI GCNGC 4 cut(s) 71, 184, 407, 552
Bme1390I CCNGG 1 cut(s) 53
BmgT120I GGNCC 1 cut(s) 24
BmiI GGNNCC 1 cut(s) 25
BmrFI CCNGG 1 cut(s) 53
BmrI ACTGGG 1 cut(s) 30
BmsI GCATC 3 cut(s) 71, 79, 417
BmuI ACTGGG 1 cut(s) 30
Bpu10I CCTNAGC 1 cut(s) 178
BpuEI CTTGAG 1 cut(s) 101
BsaBI GATNNNNATC 2 cut(s) 61, 227
BsaJI CCNNGG 2 cut(s) 52, 271
Bsc4I CCNNNNNNNGG 1 cut(s) 277
Bse1I ACTGG 1 cut(s) 25
Bse8I GATNNNNATC 2 cut(s) 61, 227
BseBI CCWGG 1 cut(s) 53
BseDI CCNNGG 2 cut(s) 52, 271
BseGI GGATG 2 cut(s) 91, 282
BseJI GATNNNNATC 2 cut(s) 61, 227
BseLI CCNNNNNNNGG 1 cut(s) 277
BseMII CTCAG 2 cut(s) 192, 582
BseNI ACTGG 1 cut(s) 25
BseXI GCAGC 2 cut(s) 194, 537
Bsh1236I CGCG 1 cut(s) 142
BshFI GGCC 2 cut(s) 26, 442
BsiSI CCGG 1 cut(s) 27
BslFI GGGAC 1 cut(s) 100
BslI CCNNNNNNNGG 1 cut(s) 277
BsmFI GGGAC 1 cut(s) 100
BsmI GAATGC 2 cut(s) 166, 185
BsnI GGCC 2 cut(s) 26, 442
Bsp143I GATC 1 cut(s) 76
BspACI CCGC 3 cut(s) 69, 142, 405
BspANI GGCC 2 cut(s) 26, 442
BspCNI CTCAG 2 cut(s) 191, 581
BspFNI CGCG 1 cut(s) 142
BspLI GGNNCC 1 cut(s) 25
BsrBI CCGCTC 1 cut(s) 69
BsrI ACTGG 1 cut(s) 25
BssECI CCNNGG 2 cut(s) 52, 271
BssMI GATC 1 cut(s) 76
BssT1I CCWWGG 1 cut(s) 271
Bst2UI CCWGG 1 cut(s) 53
BstAPI GCANNNNNTGC 1 cut(s) 547
BstC8I GCNNGC 3 cut(s) 94, 174, 493
BstDEI CTNAG 2 cut(s) 178, 568
BstF5I GGATG 2 cut(s) 91, 282
BstFNI CGCG 1 cut(s) 142
BstHHI GCGC 1 cut(s) 108
BstKTI GATC 1 cut(s) 79
BstMBI GATC 1 cut(s) 76
BstMWI GCNNNNNNNGC 3 cut(s) 182, 426, 547
BstNI CCWGG 1 cut(s) 53
BstNSI RCATGY 1 cut(s) 96
BstSCI CCNGG 1 cut(s) 51
BstUI CGCG 1 cut(s) 142
BstV1I GCAGC 2 cut(s) 194, 537
BsuRI GGCC 2 cut(s) 26, 442
BtgZI GCGATG 1 cut(s) 87
BtsCI GGATG 2 cut(s) 91, 282
Cac8I GCNNGC 3 cut(s) 94, 174, 493
CfoI GCGC 1 cut(s) 108
Cfr13I GGNCC 1 cut(s) 24
CseI GACGC 1 cut(s) 469
Csp6I GTAC 4 cut(s) 12, 196, 511, 555
CviAII CATG 5 cut(s) 93, 239, 330, 496, 542
CviJI RGCY 4 cut(s) 26, 51, 172, 442
CviKI_1 RGCY 4 cut(s) 26, 51, 172, 442
CviQI GTAC 4 cut(s) 12, 196, 511, 555
DdeI CTNAG 2 cut(s) 178, 568
DpnI GATC 1 cut(s) 78
DpnII GATC 1 cut(s) 76
DraI TTTAAA 1 cut(s) 294
EaeI YGGCCR 1 cut(s) 440
Eco130I CCWWGG 1 cut(s) 271
EcoRII CCWGG 1 cut(s) 51
EcoT14I CCWWGG 1 cut(s) 271
EcoT22I ATGCAT 2 cut(s) 64, 94
ErhI CCWWGG 1 cut(s) 271
FaeI CATG 5 cut(s) 96, 242, 333, 499, 545
FalI AAGNNNNNCTT 2 cut(s) 247, 279
FaqI GGGAC 1 cut(s) 100
FatI CATG 5 cut(s) 92, 238, 329, 495, 541
Fnu4HI GCNGC 4 cut(s) 70, 183, 406, 551
FokI GGATG 2 cut(s) 98, 289
Fsp4HI GCNGC 4 cut(s) 70, 183, 406, 551
GlaI GCGC 1 cut(s) 107
GluI GCNGC 4 cut(s) 70, 183, 406, 551
HaeIII GGCC 2 cut(s) 26, 442
HapII CCGG 1 cut(s) 27
HgaI GACGC 1 cut(s) 469
HhaI GCGC 1 cut(s) 108
Hin1II CATG 5 cut(s) 96, 242, 333, 499, 545
Hin6I GCGC 1 cut(s) 106
HinP1I GCGC 1 cut(s) 106
HpaII CCGG 1 cut(s) 27
HphI GGTGA 2 cut(s) 215, 590
Hpy166II GTNNAC 2 cut(s) 196, 477
Hpy188I TCNGA 2 cut(s) 129, 571
Hpy188III TCNNGA 3 cut(s) 80, 457, 523
Hpy8I GTNNAC 2 cut(s) 196, 477
HpyCH4V TGCA 2 cut(s) 62, 92
HpyF10VI GCNNNNNNNGC 3 cut(s) 182, 426, 547
HpyF3I CTNAG 2 cut(s) 178, 568
Hsp92II CATG 5 cut(s) 96, 242, 333, 499, 545
HspAI GCGC 1 cut(s) 106
Kzo9I GATC 1 cut(s) 76
LmnI GCTCC 1 cut(s) 333
LpnPI CCDG 6 cut(s) 6, 38, 40, 65, 193, 477
Lsp1109I GCAGC 2 cut(s) 194, 537
LweI GCATC 3 cut(s) 71, 79, 417
MaeIII GTNAC 2 cut(s) 203, 391
MalI GATC 1 cut(s) 78
MbiI CCGCTC 1 cut(s) 69
MboI GATC 1 cut(s) 76
MboII GAAGA 4 cut(s) 223, 267, 298, 415
MlsI TGGCCA 1 cut(s) 442
MluCI AATT 2 cut(s) 249, 362
MluNI TGGCCA 1 cut(s) 442
MnlI CCTC 6 cut(s) 30, 36, 76, 187, 477, 577
Mox20I TGGCCA 1 cut(s) 442
Mph1103I ATGCAT 2 cut(s) 64, 94
MscI TGGCCA 1 cut(s) 442
MseI TTAA 1 cut(s) 293
Msp20I TGGCCA 1 cut(s) 442
MspI CCGG 1 cut(s) 27
MspR9I CCNGG 1 cut(s) 53
Mva1269I GAATGC 2 cut(s) 166, 185
MvaI CCWGG 1 cut(s) 53
MvnI CGCG 1 cut(s) 142
MwoI GCNNNNNNNGC 3 cut(s) 182, 426, 547
NdeII GATC 1 cut(s) 76
NlaIII CATG 5 cut(s) 96, 242, 333, 499, 545
NlaIV GGNNCC 1 cut(s) 25
NmuCI GTSAC 2 cut(s) 203, 391
NsiI ATGCAT 2 cut(s) 64, 94
NspI RCATGY 1 cut(s) 96
PaeI GCATGC 1 cut(s) 96
PctI GAATGC 2 cut(s) 166, 185
PkrI GCNGC 4 cut(s) 71, 184, 407, 552
PsiI TTATAA 1 cut(s) 518
Psp6I CCWGG 1 cut(s) 51
PspGI CCWGG 1 cut(s) 51
PspN4I GGNNCC 1 cut(s) 25
PspPI GGNCC 1 cut(s) 24
RsaI GTAC 4 cut(s) 13, 197, 512, 556
RsaNI GTAC 4 cut(s) 12, 196, 511, 555
SaqAI TTAA 1 cut(s) 293
SatI GCNGC 4 cut(s) 70, 183, 406, 551
Sau3AI GATC 1 cut(s) 76
Sau96I GGNCC 1 cut(s) 24
ScrFI CCNGG 1 cut(s) 53
SetI ASST 2 cut(s) 273, 580
SfaNI GCATC 3 cut(s) 71, 79, 417
SmlI CTYRAG 1 cut(s) 80
SmoI CTYRAG 1 cut(s) 80
SphI GCATGC 1 cut(s) 96
Sse9I AATT 2 cut(s) 249, 362
SsiI CCGC 3 cut(s) 69, 142, 405
StyD4I CCNGG 1 cut(s) 51
StyI CCWWGG 1 cut(s) 271
TaqI TCGA 1 cut(s) 65
TasI AATT 2 cut(s) 249, 362
TatI WGTACW 1 cut(s) 195
TauI GCSGC 2 cut(s) 72, 408
Tru1I TTAA 1 cut(s) 293
Tru9I TTAA 1 cut(s) 293
TseFI GTSAC 2 cut(s) 203, 391
TseI GCWGC 2 cut(s) 182, 550
Tsp45I GTSAC 2 cut(s) 203, 391
TspDTI ATGAA 1 cut(s) 178
TspGWI ACGGA 2 cut(s) 102, 498
XceI RCATGY 1 cut(s) 96
Zsp2I ATGCAT 2 cut(s) 64, 94
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.