FvH4_1g08650

Tubulin--tyrosine ligase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0016100
Physical Location & Seq
Forward (+)
4592445 .. 4597501
5057 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g08650.t8

Sequence Viewer

Length: 2583 bp
ATGTCGGCAACCAGAATCGAAACCTACGAAGACTTCGTGAAAGTCCACGCTCTGCTCCTAACAGCCTCGGGATTGCCCCAGTCCCTGCACCGCCGCCTCTTCCAGAAGCTTCTCTCCGACAGCTTCGACGGCGGGTCCCACTTCCAAATCGAGCCCACCGAGGACGGCCGTCAGCGGCGCCTCGTCCTCACCTCCGACCACATGCCAGCAAACTCCGACGTCTTCCTCGTCGACCACGCCTGGACCTTCCGCCTCTCCGAAGCTCCCAAACAGTTGAGGGAAGTTCCGGGATTGGCTGAGAGAATGGCGAGCATAATGTGTGTGGATACTGATTTGAATTCAGAAGAGAGCCAAGTTCTAAGTGGGGATGATGCAAAACGCAGCGTTTTGGAGGTACTGGAAAGTGAGATTAGTGAAGCTAATGGGGATGTTAAGTGGTTGGAGCTTGAGGAACTTGGCATTGACAATGAAACGTTGTCGTCTTTGGACTTGTCTGCTAAGTGCCCTGGTTTGATTGCATTGTCTTTGTGTGGGAATAAAATTGACAATCTGGAGGTTGTTGTTGAGGAAGTTAGCAAATTGAAAAATTTAAGAGCGCTATGGCTGAACAACAACCCGGTTGTAGAAAATCGTGGCAATGAATTGGGGGATAGGATTCTTCGAGAATTGCCGAATCTTGAAATCTTTAACTCAAAGTTTACAGCCAATTTTGGCGAGTGGGCATTGGGGTTTTGTGGAGGAGTGTTTGACAAGGAAAACCCGGGCAGTTTTGATCTGCCTGACCGTCCGTTGCAGCAAGTCACGGATCTTGATCTTTCAGATAGATGTATTCAGAATTTAATCAATAAGGCATTTACGCCTTTTCAATTGCCATGTCTTTCATACTTGAATCTTCGAGGAAATCCATTAGATGAGACCTCACTGTCCGATTTGTTATATGTACTACGGAACTTTCCTTCATTACAATCTCTGGAGGTGGATATTCCTGGCCCTCTTGGAGAAAGTGCTCCAGAAATTCTTGAATCTCTTCCTAATCTTTCTTTACTGAATGGTGTCAATGCATCAATGATATTTGAAAATGAAAAGCAAGTGGTTGACTCAGCGCTTCAGCCACGTCTTCCTGAGTGGACTACTGATGAGCCTCTTGCTGATCGTGTTATAAACGCAATGTGGCAATATTTAATGACATATAGACTGGCGGATGAAGAGAAGATAGACGAGACCTCTGTGTTGTATGTGATGGATGAGCTAGGTTCAGCATTGAGGCACAGTGATGAGCCAAATTTCAGAGTATCTCCGTTTCTCTTTATGCCGGAGGGGACATTAGCATCAGCTGTGAGTTTCTGCATATTATGGCCAATGAAGAATGTTGAAAAAGGTTATGAATGCACTCGTGATTACCTTTTTGGTATTGGGGAGGACAAGCAACGTTCCGCCAGACTTACAGCTTGGTTCCATACACCAGAGAAGTATTTTATTCGAGAGTATGAGAAGCACTGGCAGAACTTGCGAGCAGAAAGCTTAAGACCTCCTCCGATGGAGACATGTCCAACTAAAAGTTTAAAACATACAGAAAAGCGTCCATTACGTGTGTACTCCGATATACCTCAAGTTGAAGAACTTTTGACGCGTCCAGATTTTGTATTCACATCTGAACCAAAGGATGCAGAAATCATATGGACAGGTGATCAAGTGGATGAGGATATGAAGAAAGCTGCGGGAATAACAGAAAGTCAGTTTGTAAACCAGTTTCCTTTTGAGGCTTGTATCGTCATGAAACATCATTTAGCAGATACTGTTCAGAAGGCACTTGGATCTCCTGAGTGGATGCAGCCAACCTATAATCTTGAAACACATCTATCCCAACTTATTGGTGACTATTGTGTACGTAAAAGAGACGGGCGTAACAATCTGTGGATCTTAAAACCTTGGAACATGGCACGAACTATAGATACAACTGTCACTGATAATTTATCTGCTATTATTCGGCTCATGGAGACTGGACCAAAGATATGTCAGAAGTATATTGAGCACCCAGCCTTGTTCAAAGGGAGGAAATTCGATATCCGCTACATAGTTTTACTACGGAGTTTGAGCCCTATGGAAGTATTTCTTTCAGACACTTTCTGGGTGAGACTGGCTAACAACCAATATTCCTTAGACAAGCACAGTTTTTTTGAGTATGAGACTCACTTTACTGTTATGAACTATCGTGGGATGCTGAATCACATGAACACGTCTGAATTTGTAAGGGAGTTTGAACAGGAGCACCAAGTTAAATGGTCGGATATCCACTCTAGAGTCAAAAAAATGATCAGATCGGTCTTTGAGGCAGCTGCAAAAGTGCACCCTGAAATGCACAGTCCATCATCAAGGGCCATGTATGGTGTGGATGTCATGCTTGATACTTCTTTCCAGCCAAAGTTACTGGAGGTGACTTACTGTCCTGACTGTACAAGAGCATGCAAATATGATATGGAGGCAATAGTTGCCGGAGGGGAAGTAGTTAAAGCTCGGGACTTTTATAACTATGTCTTTGGTTGTCTCTTTCTGAATGAAACCACTCATGTTACTCCATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TTL

Protein Analysis

861

Amino Acids

98.46

Weight (kDa)

5.04

Isoelectric Point (pI)

40.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SET_TTL PF25556 71 - 113 1.7e-11 Tubulin tyrosine ligase SET-like domain
SET_TTL PF25556 379 - 486 2.5e-22 Tubulin tyrosine ligase SET-like domain
TTL PF03133 578 - 709 1.8e-27 Tubulin-tyrosine ligase family
TTL PF03133 720 - 803 8.6e-12 Tubulin-tyrosine ligase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016100)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G77550
fragaria_vesca FvH4_1g08650 FvH4_1g08650
malus_domestica MD15G1217600.v1.1
prunus_persica Prupe.7G198800_v2.0.a1
pyrus_communis pycom15g19320
rosa_chinensis RchiOBHm_Chr2g0094931
rosa_laevigata RLG00000016532
rosa_multiflora Rmu_sc0004316.1_g000014
rosa_roxburghii Rroxscaffold_2G00146760
rosa_rugosa Rorug02G0048900
rosa_samantha Rh2AG095700 Rh2CG098600 Rh2DG095300
rosa_wichuraiana Rw2G007200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1160, 2526
AasI GACNNNNNNGTC 1 cut(s) 922
AatII GACGTC 1 cut(s) 222
AccB1I GGYRCC 1 cut(s) 177
AccI GTMKAC 1 cut(s) 231
AccII CGCG 1 cut(s) 1630
AciI CCGC 9 cut(s) 91, 94, 132, 175, 250, 1199, 1434, 1718, 2068
AclI AACGTT 2 cut(s) 473, 1429
AclWI GGATC 3 cut(s) 813, 1822, 1925
AcoI YGGCCR 2 cut(s) 166, 1355
AcsI RAATTY 7 cut(s) 337, 586, 835, 1014, 1282, 2057, 2243
AcuI CTGAAG 1 cut(s) 1091
AcyI GRCGYC 2 cut(s) 178, 219
AfaI GTAC 5 cut(s) 396, 942, 1595, 1887, 2455
AfeI AGCGCT 2 cut(s) 597, 1104
AflII CTTAAG 1 cut(s) 1522
AflIII ACRYGT 4 cut(s) 1544, 1588, 1628, 2235
AhdI GACNNNNNGTC 2 cut(s) 133, 2442
AjiI CACGTC 2 cut(s) 1115, 2238
AjnI CCWGG 3 cut(s) 239, 505, 985
Alw21I GWGCWC 4 cut(s) 1009, 2034, 2271, 2349
Alw26I GTCTC 8 cut(s) 908, 1214, 1535, 1890, 1991, 2128, 2180, 2549
Alw44I GTGCAC 1 cut(s) 2345
AlwI GGATC 3 cut(s) 813, 1822, 1925
AlwNI CAGNNNCTG 2 cut(s) 85, 1796
Ama87I CYCGRG 3 cut(s) 67, 760, 2514
Aor51HI AGCGCT 2 cut(s) 597, 1104
AoxI GGCC 4 cut(s) 166, 988, 1355, 2376
ApaLI GTGCAC 1 cut(s) 2345
ApeKI GCWGC 6 cut(s) 381, 793, 1715, 1831, 2333, 2336
ApoI RAATTY 7 cut(s) 337, 586, 835, 1014, 1282, 2057, 2243
Asp700I GAANNNNTTC 2 cut(s) 1026, 2109
AspLEI GCGC 3 cut(s) 180, 598, 1105
AspS9I GGNCC 5 cut(s) 135, 243, 989, 2003, 2376
AsuC2I CCSGG 4 cut(s) 288, 617, 761, 762
AsuHPI GGTGA 5 cut(s) 181, 1697, 1886, 2143, 2446
AvaI CYCGRG 3 cut(s) 67, 760, 2514
AvaII GGWCC 3 cut(s) 135, 243, 2003
BaeGI GKGCMC 2 cut(s) 506, 2349
BaeI ACNNNNGTAYC 2 cut(s) 1944, 1977
BalI TGGCCA 1 cut(s) 1357
BanI GGYRCC 1 cut(s) 177
BanII GRGCYC 2 cut(s) 156, 2099
BauI CACGAG 1 cut(s) 1392
BbsI GAAGAC 3 cut(s) 36, 214, 1109
Bbv12I GWGCWC 4 cut(s) 1009, 2034, 2271, 2349
BbvI GCAGC 6 cut(s) 393, 805, 1702, 1843, 2323, 2345
BccI CCATC 3 cut(s) 1234, 1531, 2374
BceAI ACGGC 3 cut(s) 145, 153, 181
BciT130I CCWGG 3 cut(s) 241, 507, 987
BciVI GTATCC 1 cut(s) 319
BclI TGATCA 2 cut(s) 1687, 2313
BcnI CCSGG 4 cut(s) 288, 617, 761, 762
BcoDI GTCTC 8 cut(s) 908, 1214, 1535, 1890, 1991, 2128, 2180, 2549
BfaI CTAG 2 cut(s) 1250, 2298
BfmI CTRYAG 1 cut(s) 1947
BfoI RGCGCY 3 cut(s) 181, 599, 1106
BfrI CTTAAG 1 cut(s) 1522
BfuI GTATCC 1 cut(s) 319
BisI GCNGC 8 cut(s) 94, 176, 382, 794, 1716, 1832, 2334, 2337
BlsI GCNGC 8 cut(s) 95, 177, 383, 795, 1717, 1833, 2335, 2338
Bme1390I CCNGG 7 cut(s) 241, 288, 507, 617, 761, 762, 987
Bme18I GGWCC 3 cut(s) 135, 243, 2003
BmeRI GACNNNNNGTC 2 cut(s) 133, 2442
BmeT110I CYCGRG 3 cut(s) 67, 760, 2514
BmgBI CACGTC 2 cut(s) 1115, 2238
BmgT120I GGNCC 5 cut(s) 135, 243, 989, 2003, 2376
BmiI GGNNCC 4 cut(s) 136, 137, 179, 1454
BmrFI CCNGG 7 cut(s) 241, 288, 507, 617, 761, 762, 987
BmrI ACTGGG 1 cut(s) 73
BmsI GCATC 6 cut(s) 361, 1070, 1337, 1654, 1818, 2208
BmuI ACTGGG 1 cut(s) 73
BoxI GACNNNNGTC 1 cut(s) 168
BpiI GAAGAC 3 cut(s) 36, 214, 1109
BpmI CTGGAG 4 cut(s) 572, 992, 993, 2450
BpuEI CTTGAG 2 cut(s) 467, 1593
BpuMI CCSGG 4 cut(s) 288, 617, 761, 762
BsaAI YACGTR 2 cut(s) 1589, 1889
BsaBI GATNNNNATC 1 cut(s) 810
BsaHI GRCGYC 2 cut(s) 178, 219
BsaI GGTCTC 2 cut(s) 908, 1214
BsaJI CCNNGG 5 cut(s) 66, 159, 505, 760, 1928
BsaXI ACNNNNNCTCC 2 cut(s) 2487, 2517
Bse1I ACTGG 8 cut(s) 79, 402, 1200, 1502, 1747, 2005, 2142, 2433
Bse3DI GCAATG 2 cut(s) 643, 1173
Bse8I GATNNNNATC 1 cut(s) 810
BseBI CCWGG 3 cut(s) 241, 507, 987
BseDI CCNNGG 5 cut(s) 66, 159, 505, 760, 1928
BseGI GGATG 9 cut(s) 373, 433, 1207, 1249, 1669, 1702, 1833, 2223, 2398
BseJI GATNNNNATC 1 cut(s) 810
BseMI GCAATG 2 cut(s) 643, 1173
BseMII CTCAG 4 cut(s) 288, 1113, 1113, 1812
BseNI ACTGG 8 cut(s) 79, 402, 1200, 1502, 1747, 2005, 2142, 2433
BseRI GAGGAG 2 cut(s) 753, 1521
BseSI GKGCMC 2 cut(s) 506, 2349
BseX3I CGGCCG 1 cut(s) 166
BseXI GCAGC 6 cut(s) 393, 805, 1702, 1843, 2323, 2345
BseYI CCCAGC 1 cut(s) 2035
BsgI GTGCAG 1 cut(s) 71
Bsh1236I CGCG 1 cut(s) 1630
Bsh1285I CGRYCG 1 cut(s) 169
BshFI GGCC 4 cut(s) 168, 990, 1357, 2378
BshNI GGYRCC 1 cut(s) 177
BsiEI CGRYCG 1 cut(s) 169
BsiHKAI GWGCWC 4 cut(s) 1009, 2034, 2271, 2349
BsiHKCI CYCGRG 3 cut(s) 67, 760, 2514
BsiSI CCGG 5 cut(s) 287, 617, 761, 1313, 2493
BslFI GGGAC 4 cut(s) 67, 121, 1333, 2531
BsmAI GTCTC 8 cut(s) 908, 1214, 1535, 1890, 1991, 2128, 2180, 2549
BsmBI CGTCTC 1 cut(s) 1890
BsmFI GGGAC 4 cut(s) 67, 121, 1333, 2531
BsmI GAATGC 1 cut(s) 1391
BsnI GGCC 4 cut(s) 168, 990, 1357, 2378
Bso31I GGTCTC 2 cut(s) 908, 1214
BsoBI CYCGRG 3 cut(s) 67, 760, 2514
Bsp1286I GDGCHC 7 cut(s) 156, 506, 1009, 2034, 2099, 2271, 2349
Bsp1407I TGTACA 1 cut(s) 2453
Bsp143I GATC 9 cut(s) 772, 805, 811, 1150, 1687, 1814, 1917, 2313, 2318
BspACI CCGC 9 cut(s) 91, 94, 132, 175, 250, 1199, 1434, 1718, 2068
BspANI GGCC 4 cut(s) 168, 990, 1357, 2378
BspCNI CTCAG 4 cut(s) 289, 1112, 1114, 1813
BspFNI CGCG 1 cut(s) 1630
BspHI TCATGA 1 cut(s) 1773
BspLI GGNNCC 4 cut(s) 136, 137, 179, 1454
BspPI GGATC 3 cut(s) 813, 1822, 1925
BspT107I GGYRCC 1 cut(s) 177
BspTI CTTAAG 1 cut(s) 1522
BspTNI GGTCTC 2 cut(s) 908, 1214
BsrDI GCAATG 2 cut(s) 643, 1173
BsrGI TGTACA 1 cut(s) 2453
BsrI ACTGG 8 cut(s) 79, 402, 1200, 1502, 1747, 2005, 2142, 2433
BssECI CCNNGG 5 cut(s) 66, 159, 505, 760, 1928
BssMI GATC 9 cut(s) 772, 805, 811, 1150, 1687, 1814, 1917, 2313, 2318
BssNI GRCGYC 2 cut(s) 178, 219
BssSI CACGAG 1 cut(s) 1392
BssT1I CCWWGG 1 cut(s) 1928
Bst2BI CACGAG 1 cut(s) 1392
Bst2UI CCWGG 3 cut(s) 241, 507, 987
Bst6I CTCTTC 4 cut(s) 104, 339, 1032, 1200
BstACI GRCGYC 2 cut(s) 178, 219
BstAFI CTTAAG 1 cut(s) 1522
BstAPI GCANNNNNTGC 2 cut(s) 1507, 2489
BstAUI TGTACA 1 cut(s) 2453
BstBAI YACGTR 2 cut(s) 1589, 1889
BstC8I GCNNGC 4 cut(s) 207, 310, 1512, 2464
BstDEI CTNAG 7 cut(s) 297, 359, 498, 1099, 1122, 1821, 2158
BstF5I GGATG 9 cut(s) 373, 433, 1207, 1249, 1669, 1702, 1833, 2223, 2398
BstFNI CGCG 1 cut(s) 1630
BstH2I RGCGCY 3 cut(s) 181, 599, 1106
BstHHI GCGC 3 cut(s) 180, 598, 1105
BstKTI GATC 9 cut(s) 775, 808, 814, 1153, 1690, 1817, 1920, 2316, 2321
BstMAI GTCTC 8 cut(s) 908, 1214, 1535, 1890, 1991, 2128, 2180, 2549
BstMBI GATC 9 cut(s) 772, 805, 811, 1150, 1687, 1814, 1917, 2313, 2318
BstMCI CGRYCG 1 cut(s) 169
BstMWI GCNNNNNNNGC 3 cut(s) 129, 1507, 2489
BstNI CCWGG 3 cut(s) 241, 507, 987
BstNSI RCATGY 3 cut(s) 205, 1548, 2466
BstPAI GACNNNNGTC 1 cut(s) 168
BstSCI CCNGG 7 cut(s) 239, 286, 505, 615, 759, 760, 985
BstSFI CTRYAG 1 cut(s) 1947
BstSLI GKGCMC 2 cut(s) 506, 2349
BstSNI TACGTA 1 cut(s) 1889
BstUI CGCG 1 cut(s) 1630
BstV1I GCAGC 6 cut(s) 393, 805, 1702, 1843, 2323, 2345
BstV2I GAAGAC 3 cut(s) 36, 214, 1109
BstX2I RGATCY 3 cut(s) 805, 1814, 1917
BstXI CCANNNNNNTGG 1 cut(s) 1871
BstYI RGATCY 3 cut(s) 805, 1814, 1917
BstZI CGGCCG 1 cut(s) 166
BsuI GTATCC 1 cut(s) 319
BsuRI GGCC 4 cut(s) 168, 990, 1357, 2378
BtrI CACGTC 2 cut(s) 1115, 2238
BtsCI GGATG 9 cut(s) 373, 433, 1207, 1249, 1669, 1702, 1833, 2223, 2398
BtsIMutI CAGTG 4 cut(s) 920, 1276, 1495, 1962
Cac8I GCNNGC 4 cut(s) 207, 310, 1512, 2464
CaiI CAGNNNCTG 2 cut(s) 85, 1796
CciI TCATGA 1 cut(s) 1773
CfoI GCGC 3 cut(s) 180, 598, 1105
Cfr13I GGNCC 5 cut(s) 135, 243, 989, 2003, 2376
Cfr9I CCCGGG 1 cut(s) 760
CseI GACGC 3 cut(s) 1568, 1619, 1636
Csp6I GTAC 5 cut(s) 395, 941, 1594, 1886, 2454
CviQI GTAC 5 cut(s) 395, 941, 1594, 1886, 2454
DdeI CTNAG 7 cut(s) 297, 359, 498, 1099, 1122, 1821, 2158
DinI GGCGCC 1 cut(s) 179
DpnI GATC 9 cut(s) 774, 807, 813, 1152, 1689, 1816, 1919, 2315, 2320
DpnII GATC 9 cut(s) 772, 805, 811, 1150, 1687, 1814, 1917, 2313, 2318
DraI TTTAAA 1 cut(s) 1563
DrdI GACNNNNNNGTC 1 cut(s) 922
DriI GACNNNNNGTC 2 cut(s) 133, 2442
DseDI GACNNNNNNGTC 1 cut(s) 922
EaeI YGGCCR 2 cut(s) 166, 1355
EagI CGGCCG 1 cut(s) 166
Eam1104I CTCTTC 4 cut(s) 104, 339, 1032, 1200
Eam1105I GACNNNNNGTC 2 cut(s) 133, 2442
EarI CTCTTC 4 cut(s) 104, 339, 1032, 1200
EciI GGCGGA 3 cut(s) 239, 1214, 1423
EclXI CGGCCG 1 cut(s) 166
Eco105I TACGTA 1 cut(s) 1889
Eco130I CCWWGG 1 cut(s) 1928
Eco24I GRGCYC 2 cut(s) 156, 2099
Eco31I GGTCTC 2 cut(s) 908, 1214
Eco32I GATATC 2 cut(s) 2065, 2290
Eco47I GGWCC 3 cut(s) 135, 243, 2003
Eco47III AGCGCT 2 cut(s) 597, 1104
Eco52I CGGCCG 1 cut(s) 166
Eco57I CTGAAG 1 cut(s) 1091
Eco88I CYCGRG 3 cut(s) 67, 760, 2514
EcoO109I RGGNCCY 1 cut(s) 135
EcoRI GAATTC 1 cut(s) 337
EcoRII CCWGG 3 cut(s) 239, 505, 985
EcoRV GATATC 2 cut(s) 2065, 2290
EcoT14I CCWWGG 1 cut(s) 1928
EcoT22I ATGCAT 1 cut(s) 1063
EcoT38I GRGCYC 2 cut(s) 156, 2099
EgeI GGCGCC 1 cut(s) 179
EheI GGCGCC 1 cut(s) 179
ErhI CCWWGG 1 cut(s) 1928
Esp3I CGTCTC 1 cut(s) 1890
FalI AAGNNNNNCTT 2 cut(s) 2097, 2129
FaqI GGGAC 4 cut(s) 67, 121, 1333, 2531
FauI CCCGC 2 cut(s) 125, 1711
FauNDI CATATG 1 cut(s) 1676
FbaI TGATCA 2 cut(s) 1687, 2313
FblI GTMKAC 1 cut(s) 231
Fnu4HI GCNGC 8 cut(s) 94, 176, 382, 794, 1716, 1832, 2334, 2337
FokI GGATG 9 cut(s) 380, 440, 1214, 1256, 1676, 1709, 1840, 2230, 2405
FriOI GRGCYC 2 cut(s) 156, 2099
Fsp4HI GCNGC 8 cut(s) 94, 176, 382, 794, 1716, 1832, 2334, 2337
FspBI CTAG 2 cut(s) 1250, 2298
GlaI GCGC 3 cut(s) 179, 597, 1104
GluI GCNGC 8 cut(s) 94, 176, 382, 794, 1716, 1832, 2334, 2337
GsaI CCCAGC 1 cut(s) 2039
GsuI CTGGAG 4 cut(s) 572, 992, 993, 2450
HaeII RGCGCY 3 cut(s) 181, 599, 1106
HaeIII GGCC 4 cut(s) 168, 990, 1357, 2378
HapII CCGG 5 cut(s) 287, 617, 761, 1313, 2493
HgaI GACGC 3 cut(s) 1568, 1619, 1636
HhaI GCGC 3 cut(s) 180, 598, 1105
Hin1I GRCGYC 2 cut(s) 178, 219
Hin6I GCGC 3 cut(s) 178, 596, 1103
HinP1I GCGC 3 cut(s) 178, 596, 1103
HincII GTYRAC 2 cut(s) 232, 1096
HindII GTYRAC 2 cut(s) 232, 1096
HindIII AAGCTT 2 cut(s) 107, 1519
HinfI GANTC 9 cut(s) 15, 655, 673, 889, 1022, 1097, 2188, 2224, 2301
HpaII CCGG 5 cut(s) 287, 617, 761, 1313, 2493
HphI GGTGA 5 cut(s) 181, 1697, 1886, 2143, 2446
Hpy166II GTNNAC 9 cut(s) 46, 232, 699, 1096, 1128, 1594, 1744, 1886, 2347
Hpy8I GTNNAC 9 cut(s) 46, 232, 699, 1096, 1128, 1594, 1744, 1886, 2347
Hpy99I CGWCG 3 cut(s) 131, 221, 233
HpyAV CCTTC 3 cut(s) 256, 966, 1798
HpyCH4IV ACGT 7 cut(s) 219, 473, 1114, 1429, 1588, 1888, 2237
HpyF10VI GCNNNNNNNGC 3 cut(s) 129, 1507, 2489
HpyF3I CTNAG 7 cut(s) 297, 359, 498, 1099, 1122, 1821, 2158
HpySE526I ACGT 7 cut(s) 219, 473, 1114, 1429, 1588, 1888, 2237
Hsp92I GRCGYC 2 cut(s) 178, 219
HspAI GCGC 3 cut(s) 178, 596, 1103
KasI GGCGCC 1 cut(s) 177
KflI GGGWCCC 1 cut(s) 135
Ksp22I TGATCA 2 cut(s) 1687, 2313
Kzo9I GATC 9 cut(s) 772, 805, 811, 1150, 1687, 1814, 1917, 2313, 2318
LmnI GCTCC 5 cut(s) 60, 268, 442, 1012, 2266
Lsp1109I GCAGC 6 cut(s) 393, 805, 1702, 1843, 2323, 2345
LweI GCATC 6 cut(s) 361, 1070, 1337, 1654, 1818, 2208
MaeI CTAG 2 cut(s) 1250, 2298
MaeII ACGT 7 cut(s) 219, 473, 1114, 1429, 1588, 1888, 2237
MaeIII GTNAC 7 cut(s) 799, 1874, 1904, 1960, 2424, 2434, 2569
MalI GATC 9 cut(s) 774, 807, 813, 1152, 1689, 1816, 1919, 2315, 2320
MboI GATC 9 cut(s) 772, 805, 811, 1150, 1687, 1814, 1917, 2313, 2318
MfeI CAATTG 1 cut(s) 866
MflI RGATCY 3 cut(s) 805, 1814, 1917
MhlI GDGCHC 7 cut(s) 156, 506, 1009, 2034, 2099, 2271, 2349
MlsI TGGCCA 1 cut(s) 1357
MluI ACGCGT 1 cut(s) 1628
MluNI TGGCCA 1 cut(s) 1357
Mly113I GGCGCC 1 cut(s) 178
MlyI GAGTC 3 cut(s) 1091, 2182, 2310
MmeI TCCRAC 6 cut(s) 141, 219, 240, 420, 1574, 2265
Mox20I TGGCCA 1 cut(s) 1357
Mph1103I ATGCAT 1 cut(s) 1063
MroXI GAANNNNTTC 2 cut(s) 1026, 2109
MscI TGGCCA 1 cut(s) 1357
MslI CAYNNNNRTG 1 cut(s) 1272
Msp20I TGGCCA 1 cut(s) 1357
MspA1I CMGCKG 3 cut(s) 175, 1334, 2336
MspCI CTTAAG 1 cut(s) 1522
MspI CCGG 5 cut(s) 287, 617, 761, 1313, 2493
MspR9I CCNGG 7 cut(s) 241, 288, 507, 617, 761, 762, 987
MunI CAATTG 1 cut(s) 866
Mva1269I GAATGC 1 cut(s) 1391
MvaI CCWGG 3 cut(s) 241, 507, 987
MvnI CGCG 1 cut(s) 1630
MwoI GCNNNNNNNGC 3 cut(s) 129, 1507, 2489
NarI GGCGCC 1 cut(s) 178
NciI CCSGG 4 cut(s) 288, 617, 761, 762
NdeI CATATG 1 cut(s) 1676
NdeII GATC 9 cut(s) 772, 805, 811, 1150, 1687, 1814, 1917, 2313, 2318
NlaIV GGNNCC 4 cut(s) 136, 137, 179, 1454
NmuCI GTSAC 4 cut(s) 799, 1874, 1960, 2434
NsiI ATGCAT 1 cut(s) 1063
NspI RCATGY 3 cut(s) 205, 1548, 2466
PaeI GCATGC 1 cut(s) 2466
PagI TCATGA 1 cut(s) 1773
PciI ACATGT 1 cut(s) 1544
PcsI WCGNNNNNNNCGW 4 cut(s) 24, 33, 156, 225
PctI GAATGC 1 cut(s) 1391
PdmI GAANNNNTTC 2 cut(s) 1026, 2109
PfeI GAWTC 6 cut(s) 15, 655, 673, 889, 1022, 2224
PfoI TCCNGGA 1 cut(s) 286
PkrI GCNGC 8 cut(s) 95, 177, 383, 795, 1717, 1833, 2335, 2338
PleI GAGTC 3 cut(s) 1091, 2182, 2309
PluTI GGCGCC 1 cut(s) 181
PpsI GAGTC 3 cut(s) 1091, 2182, 2309
Ppu21I YACGTR 2 cut(s) 1589, 1889
PpuMI RGGWCCY 1 cut(s) 135
PscI ACATGT 1 cut(s) 1544
PshAI GACNNNNGTC 1 cut(s) 168
PsiI TTATAA 2 cut(s) 1160, 2526
Psp1406I AACGTT 2 cut(s) 473, 1429
Psp5II RGGWCCY 1 cut(s) 135
Psp6I CCWGG 3 cut(s) 239, 505, 985
PspFI CCCAGC 1 cut(s) 2035
PspGI CCWGG 3 cut(s) 239, 505, 985
PspN4I GGNNCC 4 cut(s) 136, 137, 179, 1454
PspPI GGNCC 5 cut(s) 135, 243, 989, 2003, 2376
PspPPI RGGWCCY 1 cut(s) 135
PsrI GAACNNNNNNTAC 2 cut(s) 1936, 1968
PstNI CAGNNNCTG 2 cut(s) 85, 1796
PsuI RGATCY 3 cut(s) 805, 1814, 1917
PvuII CAGCTG 2 cut(s) 1334, 2336
RsaI GTAC 5 cut(s) 396, 942, 1595, 1887, 2455
RsaNI GTAC 5 cut(s) 395, 941, 1594, 1886, 2454
RseI CAYNNNNRTG 1 cut(s) 1272
SalI GTCGAC 1 cut(s) 230
SatI GCNGC 8 cut(s) 94, 176, 382, 794, 1716, 1832, 2334, 2337
Sau3AI GATC 9 cut(s) 772, 805, 811, 1150, 1687, 1814, 1917, 2313, 2318
Sau96I GGNCC 5 cut(s) 135, 243, 989, 2003, 2376
SchI GAGTC 3 cut(s) 1091, 2182, 2310
ScrFI CCNGG 7 cut(s) 241, 288, 507, 617, 761, 762, 987
SduI GDGCHC 7 cut(s) 156, 506, 1009, 2034, 2099, 2271, 2349
SfaNI GCATC 6 cut(s) 361, 1070, 1337, 1654, 1818, 2208
SfcI CTRYAG 1 cut(s) 1947
SfoI GGCGCC 1 cut(s) 179
SinI GGWCC 3 cut(s) 135, 243, 2003
SmaI CCCGGG 1 cut(s) 762
SmiMI CAYNNNNRTG 1 cut(s) 1272
SmlI CTYRAG 3 cut(s) 446, 1522, 1608
SmoI CTYRAG 3 cut(s) 446, 1522, 1608
SnaBI TACGTA 1 cut(s) 1889
SphI GCATGC 1 cut(s) 2466
SsiI CCGC 9 cut(s) 91, 94, 132, 175, 250, 1199, 1434, 1718, 2068
SspDI GGCGCC 1 cut(s) 177
SspI AATATT 2 cut(s) 1178, 2153
SspMI CTAG 2 cut(s) 1250, 2298
StyD4I CCNGG 7 cut(s) 239, 286, 505, 615, 759, 760, 985
StyI CCWWGG 1 cut(s) 1928
TaiI ACGT 7 cut(s) 222, 476, 1117, 1432, 1591, 1891, 2240
TaqI TCGA 8 cut(s) 18, 126, 150, 231, 661, 895, 1480, 2061
TaqII GACCGA 1 cut(s) 2311
TatI WGTACW 3 cut(s) 940, 1593, 2453
TauI GCSGC 2 cut(s) 96, 178
TfiI GAWTC 6 cut(s) 15, 655, 673, 889, 1022, 2224
TscAI CASTG 4 cut(s) 927, 1276, 1502, 1969
TseFI GTSAC 4 cut(s) 799, 1874, 1960, 2434
TseI GCWGC 6 cut(s) 381, 793, 1715, 1831, 2333, 2336
Tsp45I GTSAC 4 cut(s) 799, 1874, 1960, 2434
TspGWI ACGGA 5 cut(s) 777, 818, 961, 1287, 2101
TspMI CCCGGG 1 cut(s) 760
TspRI CASTG 4 cut(s) 927, 1276, 1502, 1969
Vha464I CTTAAG 1 cut(s) 1522
VneI GTGCAC 1 cut(s) 2345
VpaK11BI GGWCC 3 cut(s) 135, 243, 2003
XapI RAATTY 7 cut(s) 337, 586, 835, 1014, 1282, 2057, 2243
XbaI TCTAGA 1 cut(s) 2297
XceI RCATGY 3 cut(s) 205, 1548, 2466
XcmI CCANNNNNNNNNTGG 2 cut(s) 359, 2386
XmaI CCCGGG 1 cut(s) 760
XmiI GTMKAC 1 cut(s) 231
XmnI GAANNNNTTC 2 cut(s) 1026, 2109
XspI CTAG 2 cut(s) 1250, 2298
ZraI GACGTC 1 cut(s) 220
Zsp2I ATGCAT 1 cut(s) 1063
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.