MD15G1217600.v1.1

Tubulin--tyrosine ligase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0016100
Physical Location & Seq
Forward (+)
17533126 .. 17538330
5205 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1217600.v1.1.491

Sequence Viewer

Length: 2691 bp
ATGTTCCTCTTCTTTCTCGCTCTCAGCCGCCATAAACAGCGAGAAACAGTCACAGACTTCACAGCAGTGACACTGAAACCTCTTCCCTCTCAATCCAATCCCACAGTCGCAATGTCGAAAATCGAAACCTACGAGGACTTCGTCAAAGTTCACGGCCTGCTCCTGGCTGCCTCGGGACTACCGCAGTCCCTCCACCGCCAGCTCTTCCAGAAGCTTCTATCGGAGAGCTTCGACGGCGGGTCCCACTTCCAAATCGAGCCGACGGAAGACGGCCACCAGCGGCGCCTCGTTTTGACCTCCGACTCCATGCCCGCACACTCCGACGTCTTTCTCGTCGACCACGCCTGGACTTTCCGCCTTTCCGACGCTTACAAACAGTTGATGGAAGTTCCGGGATTGGCTGAGAGGATGGCGGCGATAATGTGCGTGGATACCGATTTGAATTCCGATTCGGAGGAGAATCGGGAGAGCAATGGAAAACGCAGCGTTTTGGAGGTGATGGAAAGTGAAATCACTGAAGCCAGAGGGAATGTGAGGTGGCTGGAGCTTGAAGACCTTGACATGGACGATGAAACGCTGTCGTCTTTGGACTTAGCTGCCAAGTTCCCAGATTTGCTTGCATTGTCTTTGTGTGGAAATAAGCTTGAGAGTGTGGAAGTAGTTGTTCAGGAAGTTACGAAATTCAAAAATCTGAAAGCTTTGTGGCTGGACAATAATCCAGTTGTACAAAATCGTGGCGATGAATTGGCGGATAAATTTCTGCGGGAAATGCCAAGTCTTGAAATATATAACTCAAGATTTACCTCCAATTTTGGTGAGTGGGCAGTGGGGTTTTGTGGAGGAGTATACGACAAGGAAAATCCAGGCAGCATTGATCAGCCTGACAACGCGGTGCATCACGTCACTGATCTTGATCTTTCGAATAGAAGTATTCACAACATATTCAATAAGTTTCAGGCATTTTCACCTGCTCAACTTCCATGTCTTTCATACTTGAATCTTCATGGAAATCCACTAGATGAGACCTCAGCGGGTGACTTGTTACACTTACTCAGGAATTTTCCTTCCTTACAGTCTCTGGAGGTGGATATTCCTGGTCCTCTTGGAGAAAGCGCTGTACAAATTCTTGAATCTCTTCCTAATGTTTCGGTGCTGAATGGTGTTAATGCATCAAAAGTACTGGAAACTGAAAAGGATGTAGTTGACTCAGCACTTCAACCTCGTCTTCCTGAGTGGTCTGCGGATGAGCCTCTTACTGATCGTGTTATAAGTGCAATGTGGCTGTACTTAATGACATATCGACTTGCAGATGAGGAGAAGATAGATGAGACCTCTGTATGGTATGTGATGGATGAGCTTGGTTCAGCTTTGCGGCATAGTGATGAGCCAAATTTCAGAGTGGCTCCTTTTCTGTTCATGCAAGAGGGGACACTAGCATCCGCTGTGAGCTTCACTATATTATGGCCAATCCAAAATGTTCAAAAAGGTTATGAGTGCACACGTGATTATCTTTTTGGTATTGGAGAAGACAAACAACGTTCTGCTAGACTGACAGCTTGGTTTCATACACCAGAGAATTATTTCATTCGAGAGTATGAAAAGCACCGGATGAGCTTGAAATCAAAAAATTTACCCTCACAGTCATCAGAGCCCTGTCCGGCAAGAAGCCTGCTTCGTAGTGAAACATCAGCTTTACGTGTGTACACTGATATACCTCAAGTAGAAGAACTTTTGACTCGTCCTGAGTTTGTAATCACCACTGATCTGAAGGATGCCGATATCATATGGACGGGTACACAGGTAGATGAGGATATGAAGAAGGCTACTGGAATAACAGAAAATCAGTACGTAAATCAATTCCCTTTTGAGGCTTGTATTGTGATGAAACATCATTTGGCAGAGACTATTCAAAAGGCACATGGATTTCCTGACTGGCTGCAGCCTACCTATAATCTTGAAACACATCTATCTCAACTTATTGGTGATTATTGTGTACGTAAGCGAGATGGACTTAACAATCTATGGATCTTAAAACCTTGGAATATGGCACGAACTATAGATACAACTGTCACTAGTAATATATCTGCTATTATCCGGCTCATGGAAACTGGACCAAAGATATGTCAGAAGTATATTGAGCACCCTGCTTTGTTCCAAGGGAGAAAGTTTGATATCCGCTACCTAGTTTTAGTTCGGAGTATGAACCCTCTGGAAATCTTTCTTGCGGACACTTTCTGGGTTAGATTGGCAAACAACCAATATTCTTTAGACAAGCACAGTTTATTTGAATATGAGACTCACTTTACTGTGATGAACTACCGCGGGACATTGAATCACAAGAACACATCGGAATTTGTGATCGAATTTGAACAGGAGCACAAAGTTAACTGGTTGGATATTCATTCGCGGGTGACGAAGATGATACGATCAGTTTTTGAGGCAGCTGCGCAAGTACATCCAGAGATGCATAGTCCAACATCGAGGGCCATGTACGGGGTGGATGTCATGCTTGATAGTTGTTTCCAGCCAAAGTTACTGGAGGTGACCTATTGTCCGGACTGCGGAAGGGCATGCAAATACGATATGAACTCTATCGTGGGAGACCAGGAATTAGTTAGAGCTCGCAACTTTTACAACTATGTTTTCGGTTGTCTCTTTTTGAACGAAACCACTCATGTTAGCCCCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TTL

Protein Analysis

897

Amino Acids

102.36

Weight (kDa)

5.1

Isoelectric Point (pI)

42.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SET_TTL PF25556 106 - 148 3.9e-11 Tubulin tyrosine ligase SET-like domain
SET_TTL PF25556 415 - 522 1.7e-24 Tubulin tyrosine ligase SET-like domain
TTL PF03133 614 - 863 4.7e-51 Tubulin-tyrosine ligase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016100)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G77550
fragaria_vesca FvH4_1g08650 FvH4_1g08650
malus_domestica MD15G1217600.v1.1
prunus_persica Prupe.7G198800_v2.0.a1
pyrus_communis pycom15g19320
rosa_chinensis RchiOBHm_Chr2g0094931
rosa_laevigata RLG00000016532
rosa_multiflora Rmu_sc0004316.1_g000014
rosa_roxburghii Rroxscaffold_2G00146760
rosa_rugosa Rorug02G0048900
rosa_samantha Rh2AG095700 Rh2CG098600 Rh2DG095300
rosa_wichuraiana Rw2G007200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1268
AarI CACCTGC 1 cut(s) 976
AatII GACGTC 1 cut(s) 327
Acc16I TGCGCA 1 cut(s) 2448
Acc36I ACCTGC 1 cut(s) 976
AccB1I GGYRCC 1 cut(s) 282
AccI GTMKAC 2 cut(s) 336, 846
AccII CGCG 3 cut(s) 890, 2322, 2407
AccIII TCCGGA 1 cut(s) 2554
AclI AACGTT 1 cut(s) 1537
AclWI GGATC 1 cut(s) 2033
AcoI YGGCCR 2 cut(s) 271, 1463
AcsI RAATTY 9 cut(s) 442, 680, 755, 1057, 1122, 1390, 1627, 2351, 2363
AcuI CTGAAG 2 cut(s) 537, 1787
AcvI CACGTG 1 cut(s) 1502
AcyI GRCGYC 2 cut(s) 283, 324
AfeI AGCGCT 1 cut(s) 1114
AfiI CCNNNNNNNGG 7 cut(s) 163, 562, 1338, 1867, 2101, 2493, 2561
AflIII ACRYGT 2 cut(s) 1499, 1696
AhdI GACNNNNNGTC 2 cut(s) 238, 2550
AhlI ACTAGT 1 cut(s) 2072
AjiI CACGTC 1 cut(s) 901
AjnI CCWGG 5 cut(s) 162, 344, 862, 1093, 2604
AjuI GAANNNNNNNTTGG 2 cut(s) 1877, 1909
AleI CACNNNNGTG 1 cut(s) 65
Alw21I GWGCWC 4 cut(s) 1499, 2142, 2379, 2623
Alw26I GTCTC 7 cut(s) 1016, 1080, 1322, 1895, 2288, 2595, 2657
Alw44I GTGCAC 1 cut(s) 1495
AlwI GGATC 1 cut(s) 2033
AlwNI CAGNNNCTG 1 cut(s) 1078
Ama87I CYCGRG 1 cut(s) 172
Aor13HI TCCGGA 1 cut(s) 2554
Aor51HI AGCGCT 1 cut(s) 1114
AoxI GGCC 4 cut(s) 154, 271, 1463, 2484
ApaLI GTGCAC 1 cut(s) 1495
ApeKI GCWGC 8 cut(s) 167, 483, 596, 867, 1936, 1939, 2441, 2444
ApoI RAATTY 9 cut(s) 442, 680, 755, 1057, 1122, 1390, 1627, 2351, 2363
Asp700I GAANNNNTTC 3 cut(s) 1134, 1580, 2217
AspLEI GCGC 3 cut(s) 285, 1115, 2449
AspS9I GGNCC 4 cut(s) 240, 1097, 2111, 2484
AsuC2I CCSGG 1 cut(s) 393
AsuHPI GGTGA 8 cut(s) 508, 827, 957, 1046, 1747, 1994, 2422, 2554
AsuII TTCGAA 1 cut(s) 920
AvaI CYCGRG 1 cut(s) 172
AvaII GGWCC 3 cut(s) 240, 1097, 2111
BaeGI GKGCMC 1 cut(s) 1499
BaeI ACNNNNGTAYC 4 cut(s) 1785, 1818, 2052, 2085
BalI TGGCCA 1 cut(s) 1465
BanI GGYRCC 1 cut(s) 282
BanII GRGCYC 2 cut(s) 1653, 2623
BbrPI CACGTG 1 cut(s) 1502
BbsI GAAGAC 4 cut(s) 273, 558, 1217, 1533
Bbv12I GWGCWC 4 cut(s) 1499, 2142, 2379, 2623
BbvCI CCTCAGC 1 cut(s) 1027
BbvI GCAGC 8 cut(s) 154, 495, 583, 879, 1923, 1951, 2431, 2453
BccI CCATC 5 cut(s) 376, 403, 493, 1342, 2000
BceAI ACGGC 3 cut(s) 169, 250, 286
BciT130I CCWGG 5 cut(s) 164, 346, 864, 1095, 2606
BciVI GTATCC 1 cut(s) 424
BclI TGATCA 1 cut(s) 874
BcnI CCSGG 1 cut(s) 393
BcoDI GTCTC 7 cut(s) 1016, 1080, 1322, 1895, 2288, 2595, 2657
BcuI ACTAGT 1 cut(s) 2072
BfaI CTAG 5 cut(s) 1016, 1433, 1545, 2073, 2183
BfmI CTRYAG 2 cut(s) 1937, 2055
BfoI RGCGCY 2 cut(s) 286, 1116
BfuAI ACCTGC 1 cut(s) 976
BfuI GTATCC 1 cut(s) 424
BmcAI AGTACT 1 cut(s) 1179
Bme1390I CCNGG 6 cut(s) 164, 346, 393, 864, 1095, 2606
Bme18I GGWCC 3 cut(s) 240, 1097, 2111
BmeRI GACNNNNNGTC 2 cut(s) 238, 2550
BmeT110I CYCGRG 1 cut(s) 172
BmgBI CACGTC 1 cut(s) 901
BmgT120I GGNCC 4 cut(s) 240, 1097, 2111, 2484
BmiI GGNNCC 4 cut(s) 241, 242, 284, 1404
BmrFI CCNGG 6 cut(s) 164, 346, 393, 864, 1095, 2606
BmsI GCATC 5 cut(s) 904, 1178, 1445, 1762, 2454
BpiI GAAGAC 4 cut(s) 273, 558, 1217, 1533
BpmI CTGGAG 3 cut(s) 563, 1100, 2558
Bpu10I CCTNAGC 1 cut(s) 1027
Bpu14I TTCGAA 1 cut(s) 920
BpuEI CTTGAG 3 cut(s) 665, 778, 1701
BpuMI CCSGG 1 cut(s) 393
BsaAI YACGTR 4 cut(s) 1502, 1697, 1849, 1997
BsaBI GATNNNNATC 1 cut(s) 912
BsaHI GRCGYC 2 cut(s) 283, 324
BsaI GGTCTC 3 cut(s) 1016, 1322, 2595
BsaJI CCNNGG 4 cut(s) 171, 2036, 2155, 2320
BsaWI WCCGGW 2 cut(s) 1605, 2554
Bsc4I CCNNNNNNNGG 7 cut(s) 163, 562, 1338, 1867, 2101, 2493, 2561
Bse1I ACTGG 7 cut(s) 719, 1185, 1831, 1937, 2113, 2393, 2541
Bse3DI GCAATG 3 cut(s) 117, 478, 1281
Bse8I GATNNNNATC 1 cut(s) 912
BseAI TCCGGA 1 cut(s) 2554
BseBI CCWGG 5 cut(s) 164, 346, 864, 1095, 2606
BseDI CCNNGG 4 cut(s) 171, 2036, 2155, 2320
BseGI GGATG 9 cut(s) 414, 1201, 1249, 1357, 1436, 1614, 1777, 2455, 2506
BseJI GATNNNNATC 1 cut(s) 912
BseLI CCNNNNNNNGG 7 cut(s) 163, 562, 1338, 1867, 2101, 2493, 2561
BseMI GCAATG 3 cut(s) 117, 478, 1281
BseMII CTCAG 7 cut(s) 37, 393, 1041, 1066, 1221, 1221, 1734
BseNI ACTGG 7 cut(s) 719, 1185, 1831, 1937, 2113, 2393, 2541
BseRI GAGGAG 3 cut(s) 470, 855, 1328
BseSI GKGCMC 1 cut(s) 1499
BseXI GCAGC 8 cut(s) 154, 495, 583, 879, 1923, 1951, 2431, 2453
Bsh1236I CGCG 3 cut(s) 890, 2322, 2407
BshFI GGCC 4 cut(s) 156, 273, 1465, 2486
BshNI GGYRCC 1 cut(s) 282
BsiHKAI GWGCWC 4 cut(s) 1499, 2142, 2379, 2623
BsiHKCI CYCGRG 1 cut(s) 172
BsiSI CCGG 5 cut(s) 392, 1606, 1658, 2095, 2555
BslFI GGGAC 5 cut(s) 172, 189, 226, 1441, 2338
BslI CCNNNNNNNGG 7 cut(s) 163, 562, 1338, 1867, 2101, 2493, 2561
BsmAI GTCTC 7 cut(s) 1016, 1080, 1322, 1895, 2288, 2595, 2657
BsmFI GGGAC 5 cut(s) 172, 189, 226, 1441, 2338
BsnI GGCC 4 cut(s) 156, 273, 1465, 2486
Bso31I GGTCTC 3 cut(s) 1016, 1322, 2595
BsoBI CYCGRG 1 cut(s) 172
Bsp119I TTCGAA 1 cut(s) 920
Bsp1286I GDGCHC 5 cut(s) 1499, 1653, 2142, 2379, 2623
Bsp13I TCCGGA 1 cut(s) 2554
Bsp1407I TGTACA 3 cut(s) 724, 1117, 1701
Bsp143I GATC 8 cut(s) 874, 907, 913, 1258, 1762, 2025, 2358, 2426
BspANI GGCC 4 cut(s) 156, 273, 1465, 2486
BspCNI CTCAG 7 cut(s) 36, 394, 1040, 1065, 1220, 1222, 1735
BspEI TCCGGA 1 cut(s) 2554
BspFNI CGCG 3 cut(s) 890, 2322, 2407
BspLI GGNNCC 4 cut(s) 241, 242, 284, 1404
BspMAI CTGCAG 1 cut(s) 1941
BspMI ACCTGC 1 cut(s) 976
BspPI GGATC 1 cut(s) 2033
BspQI GCTCTTC 1 cut(s) 209
BspT104I TTCGAA 1 cut(s) 920
BspT107I GGYRCC 1 cut(s) 282
BspTNI GGTCTC 3 cut(s) 1016, 1322, 2595
BsrDI GCAATG 3 cut(s) 117, 478, 1281
BsrGI TGTACA 3 cut(s) 724, 1117, 1701
BsrI ACTGG 7 cut(s) 719, 1185, 1831, 1937, 2113, 2393, 2541
BssECI CCNNGG 4 cut(s) 171, 2036, 2155, 2320
BssMI GATC 8 cut(s) 874, 907, 913, 1258, 1762, 2025, 2358, 2426
BssNAI GTATAC 1 cut(s) 847
BssNI GRCGYC 2 cut(s) 283, 324
BssT1I CCWWGG 2 cut(s) 2036, 2155
Bst1107I GTATAC 1 cut(s) 847
Bst2UI CCWGG 5 cut(s) 164, 346, 864, 1095, 2606
Bst4CI ACNGT 8 cut(s) 49, 106, 378, 1074, 1641, 2068, 2279, 2308
Bst6I CTCTTC 4 cut(s) 14, 87, 209, 1140
BstACI GRCGYC 2 cut(s) 283, 324
BstAUI TGTACA 3 cut(s) 724, 1117, 1701
BstBAI YACGTR 4 cut(s) 1502, 1697, 1849, 1997
BstBI TTCGAA 1 cut(s) 920
BstC8I GCNNGC 7 cut(s) 158, 200, 312, 618, 1670, 2572, 2623
BstDEI CTNAG 8 cut(s) 23, 402, 592, 1027, 1052, 1207, 1230, 1743
BstDSI CCRYGG 1 cut(s) 2320
BstEII GGTNACC 1 cut(s) 2542
BstF5I GGATG 9 cut(s) 414, 1201, 1249, 1357, 1436, 1614, 1777, 2455, 2506
BstFNI CGCG 3 cut(s) 890, 2322, 2407
BstH2I RGCGCY 2 cut(s) 286, 1116
BstHHI GCGC 3 cut(s) 285, 1115, 2449
BstKTI GATC 8 cut(s) 877, 910, 916, 1261, 1765, 2028, 2361, 2429
BstMAI GTCTC 7 cut(s) 1016, 1080, 1322, 1895, 2288, 2595, 2657
BstMBI GATC 8 cut(s) 874, 907, 913, 1258, 1762, 2025, 2358, 2426
BstMWI GCNNNNNNNGC 2 cut(s) 234, 769
BstNI CCWGG 5 cut(s) 164, 346, 864, 1095, 2606
BstNSI RCATGY 1 cut(s) 2574
BstPI GGTNACC 1 cut(s) 2542
BstSCI CCNGG 6 cut(s) 162, 344, 391, 862, 1093, 2604
BstSFI CTRYAG 2 cut(s) 1937, 2055
BstSLI GKGCMC 1 cut(s) 1499
BstSNI TACGTA 2 cut(s) 1849, 1997
BstUI CGCG 3 cut(s) 890, 2322, 2407
BstV1I GCAGC 8 cut(s) 154, 495, 583, 879, 1923, 1951, 2431, 2453
BstV2I GAAGAC 4 cut(s) 273, 558, 1217, 1533
BstX2I RGATCY 1 cut(s) 2025
BstYI RGATCY 1 cut(s) 2025
BstZ17I GTATAC 1 cut(s) 847
BsuI GTATCC 1 cut(s) 424
BsuRI GGCC 4 cut(s) 156, 273, 1465, 2486
BtgI CCRYGG 1 cut(s) 2320
BtgZI GCGATG 1 cut(s) 753
BtrI CACGTC 1 cut(s) 901
BtsCI GGATG 9 cut(s) 414, 1201, 1249, 1357, 1436, 1614, 1777, 2455, 2506
BtsI GCAGTG 2 cut(s) 72, 831
BtsIMutI CAGTG 7 cut(s) 71, 72, 513, 831, 903, 1704, 1758
BveI ACCTGC 1 cut(s) 976
Cac8I GCNNGC 7 cut(s) 158, 200, 312, 618, 1670, 2572, 2623
CaiI CAGNNNCTG 1 cut(s) 1078
CfoI GCGC 3 cut(s) 285, 1115, 2449
Cfr13I GGNCC 4 cut(s) 240, 1097, 2111, 2484
Cfr42I CCGCGG 1 cut(s) 2323
CseI GACGC 1 cut(s) 374
DdeI CTNAG 8 cut(s) 23, 402, 592, 1027, 1052, 1207, 1230, 1743
DinI GGCGCC 1 cut(s) 284
DpnI GATC 8 cut(s) 876, 909, 915, 1260, 1764, 2027, 2360, 2428
DpnII GATC 8 cut(s) 874, 907, 913, 1258, 1762, 2025, 2358, 2426
DriI GACNNNNNGTC 2 cut(s) 238, 2550
EaeI YGGCCR 2 cut(s) 271, 1463
Eam1104I CTCTTC 4 cut(s) 14, 87, 209, 1140
Eam1105I GACNNNNNGTC 2 cut(s) 238, 2550
EarI CTCTTC 4 cut(s) 14, 87, 209, 1140
EciI GGCGGA 2 cut(s) 344, 764
Ecl136II GAGCTC 1 cut(s) 2621
Eco105I TACGTA 2 cut(s) 1849, 1997
Eco130I CCWWGG 2 cut(s) 2036, 2155
Eco24I GRGCYC 2 cut(s) 1653, 2623
Eco31I GGTCTC 3 cut(s) 1016, 1322, 2595
Eco32I GATATC 2 cut(s) 1780, 2173
Eco47I GGWCC 3 cut(s) 240, 1097, 2111
Eco47III AGCGCT 1 cut(s) 1114
Eco53kI GAGCTC 1 cut(s) 2621
Eco57I CTGAAG 2 cut(s) 537, 1787
Eco72I CACGTG 1 cut(s) 1502
Eco88I CYCGRG 1 cut(s) 172
Eco91I GGTNACC 1 cut(s) 2542
EcoICRI GAGCTC 1 cut(s) 2621
EcoO109I RGGNCCY 1 cut(s) 240
EcoO65I GGTNACC 1 cut(s) 2542
EcoRI GAATTC 1 cut(s) 442
EcoRII CCWGG 5 cut(s) 162, 344, 862, 1093, 2604
EcoRV GATATC 2 cut(s) 1780, 2173
EcoT14I CCWWGG 2 cut(s) 2036, 2155
EcoT22I ATGCAT 2 cut(s) 1171, 2469
EcoT38I GRGCYC 2 cut(s) 1653, 2623
EgeI GGCGCC 1 cut(s) 284
EheI GGCGCC 1 cut(s) 284
ErhI CCWWGG 2 cut(s) 2036, 2155
FaqI GGGAC 5 cut(s) 172, 189, 226, 1441, 2338
FauI CCCGC 6 cut(s) 230, 319, 756, 1024, 2315, 2400
FauNDI CATATG 1 cut(s) 1784
FbaI TGATCA 1 cut(s) 874
FblI GTMKAC 2 cut(s) 336, 846
FokI GGATG 9 cut(s) 421, 1208, 1256, 1364, 1423, 1621, 1784, 2442, 2513
FriOI GRGCYC 2 cut(s) 1653, 2623
FspBI CTAG 5 cut(s) 1016, 1433, 1545, 2073, 2183
FspI TGCGCA 1 cut(s) 2448
GlaI GCGC 3 cut(s) 284, 1114, 2448
GsuI CTGGAG 3 cut(s) 563, 1100, 2558
HaeII RGCGCY 2 cut(s) 286, 1116
HaeIII GGCC 4 cut(s) 156, 273, 1465, 2486
HapII CCGG 5 cut(s) 392, 1606, 1658, 2095, 2555
HgaI GACGC 1 cut(s) 374
HhaI GCGC 3 cut(s) 285, 1115, 2449
Hin1I GRCGYC 2 cut(s) 283, 324
Hin6I GCGC 3 cut(s) 283, 1113, 2447
HinP1I GCGC 3 cut(s) 283, 1113, 2447
HincII GTYRAC 3 cut(s) 337, 1204, 2386
HindII GTYRAC 3 cut(s) 337, 1204, 2386
HindIII AAGCTT 3 cut(s) 212, 641, 696
HinfI GANTC 9 cut(s) 302, 449, 460, 997, 1130, 1205, 1735, 2296, 2332
HpaI GTTAAC 1 cut(s) 2386
HpaII CCGG 5 cut(s) 392, 1606, 1658, 2095, 2555
HphI GGTGA 8 cut(s) 508, 827, 957, 1046, 1747, 1994, 2422, 2554
Hpy99I CGWCG 5 cut(s) 236, 265, 326, 338, 368
HpyAV CCTTC 4 cut(s) 1074, 1762, 1813, 2559
HpyCH4III ACNGT 8 cut(s) 49, 106, 378, 1074, 1641, 2068, 2279, 2308
HpyCH4IV ACGT 7 cut(s) 324, 900, 1501, 1537, 1696, 1848, 1996
HpyF10VI GCNNNNNNNGC 2 cut(s) 234, 769
HpyF3I CTNAG 8 cut(s) 23, 402, 592, 1027, 1052, 1207, 1230, 1743
HpySE526I ACGT 7 cut(s) 324, 900, 1501, 1537, 1696, 1848, 1996
Hsp92I GRCGYC 2 cut(s) 283, 324
HspAI GCGC 3 cut(s) 283, 1113, 2447
KasI GGCGCC 1 cut(s) 282
KflI GGGWCCC 1 cut(s) 240
Kpn2I TCCGGA 1 cut(s) 2554
Ksp22I TGATCA 1 cut(s) 874
KspAI GTTAAC 1 cut(s) 2386
KspI CCGCGG 1 cut(s) 2323
Kzo9I GATC 8 cut(s) 874, 907, 913, 1258, 1762, 2025, 2358, 2426
LguI GCTCTTC 1 cut(s) 209
LmnI GCTCC 4 cut(s) 165, 544, 1408, 2374
Lsp1109I GCAGC 8 cut(s) 154, 495, 583, 879, 1923, 1951, 2431, 2453
LweI GCATC 5 cut(s) 904, 1178, 1445, 1762, 2454
MaeI CTAG 5 cut(s) 1016, 1433, 1545, 2073, 2183
MaeII ACGT 7 cut(s) 324, 900, 1501, 1537, 1696, 1848, 1996
MalI GATC 8 cut(s) 876, 909, 915, 1260, 1764, 2027, 2360, 2428
MboI GATC 8 cut(s) 874, 907, 913, 1258, 1762, 2025, 2358, 2426
MflI RGATCY 1 cut(s) 2025
MhlI GDGCHC 5 cut(s) 1499, 1653, 2142, 2379, 2623
MlsI TGGCCA 1 cut(s) 1465
MluNI TGGCCA 1 cut(s) 1465
Mly113I GGCGCC 1 cut(s) 283
MlyI GAGTC 4 cut(s) 296, 1199, 1729, 2290
MmeI TCCRAC 5 cut(s) 324, 345, 387, 2373, 2498
Mox20I TGGCCA 1 cut(s) 1465
Mph1103I ATGCAT 2 cut(s) 1171, 2469
MroI TCCGGA 1 cut(s) 2554
MroXI GAANNNNTTC 3 cut(s) 1134, 1580, 2217
MscI TGGCCA 1 cut(s) 1465
MseI TTAA 5 cut(s) 1164, 1289, 2013, 2030, 2385
MslI CAYNNNNRTG 2 cut(s) 65, 1380
Msp20I TGGCCA 1 cut(s) 1465
MspA1I CMGCKG 5 cut(s) 280, 1031, 1442, 2322, 2444
MspI CCGG 5 cut(s) 392, 1606, 1658, 2095, 2555
MspR9I CCNGG 6 cut(s) 164, 346, 393, 864, 1095, 2606
MvaI CCWGG 5 cut(s) 164, 346, 864, 1095, 2606
MvnI CGCG 3 cut(s) 890, 2322, 2407
MwoI GCNNNNNNNGC 2 cut(s) 234, 769
NarI GGCGCC 1 cut(s) 283
NciI CCSGG 1 cut(s) 393
NdeI CATATG 1 cut(s) 1784
NdeII GATC 8 cut(s) 874, 907, 913, 1258, 1762, 2025, 2358, 2426
NlaIV GGNNCC 4 cut(s) 241, 242, 284, 1404
NmuCI GTSAC 7 cut(s) 49, 67, 901, 1034, 2068, 2410, 2542
NsbI TGCGCA 1 cut(s) 2448
NsiI ATGCAT 2 cut(s) 1171, 2469
NspI RCATGY 1 cut(s) 2574
NspV TTCGAA 1 cut(s) 920
OliI CACNNNNGTG 1 cut(s) 65
PaeI GCATGC 1 cut(s) 2574
PaqCI CACCTGC 1 cut(s) 976
PciSI GCTCTTC 1 cut(s) 209
PcsI WCGNNNNNNNCGW 4 cut(s) 129, 138, 330, 2411
PdmI GAANNNNTTC 3 cut(s) 1134, 1580, 2217
PfeI GAWTC 5 cut(s) 449, 460, 997, 1130, 2332
PflFI GACNNNGTC 1 cut(s) 140
PfoI TCCNGGA 1 cut(s) 391
PleI GAGTC 4 cut(s) 296, 1199, 1729, 2290
PluTI GGCGCC 1 cut(s) 286
PmaCI CACGTG 1 cut(s) 1502
PmlI CACGTG 1 cut(s) 1502
PpsI GAGTC 4 cut(s) 296, 1199, 1729, 2290
Ppu21I YACGTR 4 cut(s) 1502, 1697, 1849, 1997
PpuMI RGGWCCY 1 cut(s) 240
PsiI TTATAA 1 cut(s) 1268
Psp124BI GAGCTC 1 cut(s) 2623
Psp1406I AACGTT 1 cut(s) 1537
Psp5II RGGWCCY 1 cut(s) 240
Psp6I CCWGG 5 cut(s) 162, 344, 862, 1093, 2604
PspCI CACGTG 1 cut(s) 1502
PspEI GGTNACC 1 cut(s) 2542
PspGI CCWGG 5 cut(s) 162, 344, 862, 1093, 2604
PspN4I GGNNCC 4 cut(s) 241, 242, 284, 1404
PspPI GGNCC 4 cut(s) 240, 1097, 2111, 2484
PspPPI RGGWCCY 1 cut(s) 240
PsrI GAACNNNNNNTAC 2 cut(s) 2044, 2076
PstI CTGCAG 1 cut(s) 1941
PstNI CAGNNNCTG 1 cut(s) 1078
PsuI RGATCY 1 cut(s) 2025
PsyI GACNNNGTC 1 cut(s) 140
PvuII CAGCTG 1 cut(s) 2444
RseI CAYNNNNRTG 2 cut(s) 65, 1380
SacI GAGCTC 1 cut(s) 2623
SacII CCGCGG 1 cut(s) 2323
SalI GTCGAC 1 cut(s) 335
SapI GCTCTTC 1 cut(s) 209
SaqAI TTAA 5 cut(s) 1164, 1289, 2013, 2030, 2385
Sau3AI GATC 8 cut(s) 874, 907, 913, 1258, 1762, 2025, 2358, 2426
Sau96I GGNCC 4 cut(s) 240, 1097, 2111, 2484
ScaI AGTACT 1 cut(s) 1179
SchI GAGTC 4 cut(s) 296, 1199, 1729, 2290
ScrFI CCNGG 6 cut(s) 164, 346, 393, 864, 1095, 2606
SduI GDGCHC 5 cut(s) 1499, 1653, 2142, 2379, 2623
SfaNI GCATC 5 cut(s) 904, 1178, 1445, 1762, 2454
SfcI CTRYAG 2 cut(s) 1937, 2055
SfoI GGCGCC 1 cut(s) 284
Sfr303I CCGCGG 1 cut(s) 2323
SfuI TTCGAA 1 cut(s) 920
SgrBI CCGCGG 1 cut(s) 2323
SinI GGWCC 3 cut(s) 240, 1097, 2111
SmiMI CAYNNNNRTG 2 cut(s) 65, 1380
SmlI CTYRAG 3 cut(s) 644, 793, 1716
SmoI CTYRAG 3 cut(s) 644, 793, 1716
SnaBI TACGTA 2 cut(s) 1849, 1997
SpeI ACTAGT 1 cut(s) 2072
SphI GCATGC 1 cut(s) 2574
SspDI GGCGCC 1 cut(s) 282
SspI AATATT 1 cut(s) 2261
SspMI CTAG 5 cut(s) 1016, 1433, 1545, 2073, 2183
SstI GAGCTC 1 cut(s) 2623
StyD4I CCNGG 6 cut(s) 162, 344, 391, 862, 1093, 2604
StyI CCWWGG 2 cut(s) 2036, 2155
TaaI ACNGT 8 cut(s) 49, 106, 378, 1074, 1641, 2068, 2279, 2308
TaiI ACGT 7 cut(s) 327, 903, 1504, 1540, 1699, 1851, 1999
TatI WGTACW 6 cut(s) 724, 1117, 1177, 1284, 1701, 2452
TauI GCSGC 4 cut(s) 30, 283, 416, 1375
TfiI GAWTC 5 cut(s) 449, 460, 997, 1130, 2332
Tru1I TTAA 5 cut(s) 1164, 1289, 2013, 2030, 2385
Tru9I TTAA 5 cut(s) 1164, 1289, 2013, 2030, 2385
TscAI CASTG 7 cut(s) 72, 78, 520, 831, 910, 1711, 1765
TseFI GTSAC 7 cut(s) 49, 67, 901, 1034, 2068, 2410, 2542
TseI GCWGC 8 cut(s) 167, 483, 596, 867, 1936, 1939, 2441, 2444
Tsp45I GTSAC 7 cut(s) 49, 67, 901, 1034, 2068, 2410, 2542
TspGWI ACGGA 1 cut(s) 278
TspRI CASTG 7 cut(s) 72, 78, 520, 831, 910, 1711, 1765
Tth111I GACNNNGTC 1 cut(s) 140
VneI GTGCAC 1 cut(s) 1495
VpaK11BI GGWCC 3 cut(s) 240, 1097, 2111
XapI RAATTY 9 cut(s) 442, 680, 755, 1057, 1122, 1390, 1627, 2351, 2363
XceI RCATGY 1 cut(s) 2574
XcmI CCANNNNNNNNNTGG 1 cut(s) 2494
XmiI GTMKAC 2 cut(s) 336, 846
XmnI GAANNNNTTC 3 cut(s) 1134, 1580, 2217
XspI CTAG 5 cut(s) 1016, 1433, 1545, 2073, 2183
ZraI GACGTC 1 cut(s) 325
ZrmI AGTACT 1 cut(s) 1179
Zsp2I ATGCAT 2 cut(s) 1171, 2469
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.