Prupe.7G198800_v2.0.a1

Tubulin--tyrosine ligase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0016100
Physical Location & Seq
Reverse (-)
18662671 .. 18668437
5767 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G198800.1

Sequence Viewer

Length: 2580 bp
ATGACGAGAATCGAAACCTACGAGGACTTTGTCAAAGTTCACGGTCTGCTTCTGGCAGCCTCGGGATTGCCTCAGTCACTGCACCGCCAGCTGTTCCAGAAGCTTCTTTCGGAGAGCTTCGATGGCGGGTCCCACTTCCAAATCGAGCCCACCGAGGACGGCCGTCAGCGGCGTCTTGTTTTGACCTCCGACTCCATGCCCGAACACTCCGACGTCTTCCTTATCGATCACGCCTGGACTTTCCGCCTCTCCGACGCTTACAAGCAGTTAATGGAAGTTCCTGGGTTGGCTGAGAGGATGGCGGCGATAATGTGTGTGGATGCTGATTTGAATTCTGAGTCAGAAGAGGACCGTTTGAGTGAGGGCAATGCAAAACGGAGCGTTTTGGAGGTACTGGAAAATGAGATTACTGAAGCCAACGGGAACGTGAGGTGGTTGGAGCTTGAGGACCTTGACATCGATGATGAAACGCTGTCGTCCTTAGACTTGTCTACTAAGAGCCCAGGTTTGTTTGCATTATCCTTGTGTGGAAATAAGCTTGAGAATGTGGGAGTAGTTGTTCGGGAAGTTACTAAGTTCAAAAATCTAAGAGCTCTATGGCTGAACAATAACCCAGTTGTACAAAATTGTGGTGATGAATTGGCAGATAAAGTTCTTCGAGAATTGCTGAATCTTGAAATATATAACTCAAAGTTTACCTCCAATTATGGTGAGTGGGCATTGGGGTTTTGCGGAGGAGTGTATGATAAGGAAAATCCAGGGAGCGTTGATCAGCCTGACAACGCATTGAAGCAAGTCACTGATCTTGATCTGTCAAATAGATGTATTCACAATTTGTTCAATAAGGCATTTACACCTCTTGAGCTTCCATGTCTTTCATACTTGAATCTTCGGGGAAATCCATTGGAAGAGACCGCAGTGGGTGACTTGTTGCAGTTACTCAGGAATTTTCCTTCTTTACAGTCCCTGGAGGTGGATATTCCTGGCCCTCTTGGAGAAAGTGCTGTACAAATTCTTGAATCTCTTCCTAATATTTCAGTACTAAATGATGTCAATGCATCAAAAATATTTGAAACTGAAAAGGATGTGGTTGACTCAGTGCTTCAGCCACGTCTTCCTGAGTGGACTGCAGATGAGCCACTTACTGATCGTGTTATAAATGCAATGTGGCTATATTTAATGACATATCGACTTGCAGATGAGGAGAAGATCGACGAGACCTCTGTACTATACGTGATGGATGAGCTGGGTTCAGCTTTGCGGCATAGTGATGAGCCAAATTTCAGAGTGGCACCTTTTCTCTTCATGCCAGAGGGGACACTAGCATCAGCTGTGAGCTTCTCGATATTATGGCCAATCCAGAATGTTCAAAAAGGTTATGAGTGCACTCGTGATTATCTTTTTGGTATTGGAGAGGACAAGCAACGTTCTGCCAGACTCACAGCTTGGTTCAATACACCAGAGAATTATTTTATTAGAGAGTATGAAAAGCACCAGCGGAGCTTGAAATCAAAGAATTTAACCTCTCTGACGTTGGATTCATGTCCAACAAGAAGCCTGCATCGTAGTGAAACCTCGGCTTTACGTGTTTACACTGATATACCTCAAGTAGAAGAACTGTTGACACGTCCTGAGTTTGTAATCACCACTGATCCGAAGGATGCAGAAATCATATGGACGTGTACGCAGGTAGATGAGGATATGAAGAAGGCTACAGGAATAACAGACAATCAGTACATAAACCAGTTTCCTTTCGAGGCGTGTATTGTGATGAAACATCATTTGGCAGAGACTATTCAGAAGGCGCATGGATCTCCTGAATGGCTGCAGCCTACATATAATCTTGAAACACATCTGTCTCAACTTATTGGTGACTATTGTGTACGTAAAAGAGATGGACTTAACAATCTATGGATCTTAAAACCTTGGAACATGGCACGAACTATAGATACAACTGTCACTGGTAATAGATCTGCTATCATACGGCTCATGGAGACTGGACCAAAGATATGTCAGAAGTATATTGAGCACCCAGCTTTGTTCCAAGGGAAAAAATTTGATATCCGCTACATAGTTTTAGTCCGGAGTATGAACCCTTTGGAAATATTTCTTTCAGACACTTTCTGGGTTAGATTGGCAAACAACCAATATTCTTTAGACCAGCACAGTTTGTTTGAGTATGAGACTCACTTTACTGTTATGAACTACCGAGGGACATTGAATCACAAGAACACATCAGAATTTGTGAGGGAATTTGAACAGGAGCACCAAGTTAACTGGTTGGATATTCATTCGAGAGTTAAGAAAATGATCCGATCAGTCTTTGAGGCAGCTGCACAAGTACATCCAGAGATGCATGGTACAACATCGAGGGCCATGTATGGGGTGGATGTCATGCTTGACACTTCTTTCCAGCCAAAGTTACTGGAGGTGACCTACTGTCCTGACTGTGGAAGAGCATGCAAATACGATATGGAGGCTATAGTTGGAGACAAGGAAGTAATTAGAGGCCGTGACTTTTACAATTATGTCTTTGGTTGTCTCTTTTTGAACGAAACAACTCATGTTATGCCATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TTL

Protein Analysis

860

Amino Acids

98.32

Weight (kDa)

4.96

Isoelectric Point (pI)

38.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016100)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G77550
fragaria_vesca FvH4_1g08650 FvH4_1g08650
malus_domestica MD15G1217600.v1.1
prunus_persica Prupe.7G198800_v2.0.a1
pyrus_communis pycom15g19320
rosa_chinensis RchiOBHm_Chr2g0094931
rosa_laevigata RLG00000016532
rosa_multiflora Rmu_sc0004316.1_g000014
rosa_roxburghii Rroxscaffold_2G00146760
rosa_rugosa Rorug02G0048900
rosa_samantha Rh2AG095700 Rh2CG098600 Rh2DG095300
rosa_wichuraiana Rw2G007200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1157
AatII GACGTC 1 cut(s) 216
Acc36I ACCTGC 1 cut(s) 1678
AccB1I GGYRCC 1 cut(s) 1291
AccI GTMKAC 1 cut(s) 491
AccIII TCCGGA 1 cut(s) 2082
AclI AACGTT 1 cut(s) 1426
AclWI GGATC 4 cut(s) 1646, 1819, 1922, 2305
AcoI YGGCCR 2 cut(s) 160, 1352
AcsI RAATTY 8 cut(s) 331, 946, 1011, 1279, 1516, 2054, 2240, 2252
AcuI CTGAAG 2 cut(s) 432, 1088
AcyI GRCGYC 2 cut(s) 172, 213
AfiI CCNNNNNNNGG 3 cut(s) 973, 2382, 2450
AflIII ACRYGT 3 cut(s) 1585, 1625, 1679
AhdI GACNNNNNGTC 1 cut(s) 2439
AjiI CACGTC 3 cut(s) 1112, 1628, 1680
AjnI CCWGG 6 cut(s) 233, 280, 502, 757, 966, 982
AjuI GAANNNNNNNTTGG 2 cut(s) 1766, 1798
AloI GAACNNNNNNTCC 2 cut(s) 543, 575
Alw21I GWGCWC 4 cut(s) 595, 1388, 2031, 2268
Alw26I GTCTC 8 cut(s) 905, 1211, 1784, 1863, 1988, 2177, 2484, 2546
Alw44I GTGCAC 1 cut(s) 1384
AlwI GGATC 4 cut(s) 1646, 1819, 1922, 2305
AlwNI CAGNNNCTG 2 cut(s) 79, 967
Ama87I CYCGRG 1 cut(s) 61
Aor13HI TCCGGA 1 cut(s) 2082
AoxI GGCC 5 cut(s) 160, 985, 1352, 2373, 2509
ApaLI GTGCAC 1 cut(s) 1384
ApeKI GCWGC 5 cut(s) 56, 1825, 1828, 2330, 2333
ApoI RAATTY 8 cut(s) 331, 946, 1011, 1279, 1516, 2054, 2240, 2252
Asp700I GAANNNNTTC 2 cut(s) 1023, 2106
AspLEI GCGC 1 cut(s) 1807
AspS9I GGNCC 6 cut(s) 129, 349, 448, 986, 2000, 2373
AsuHPI GGTGA 6 cut(s) 644, 722, 935, 1636, 1883, 2443
AvaI CYCGRG 1 cut(s) 61
AvaII GGWCC 4 cut(s) 129, 349, 448, 2000
BaeGI GKGCMC 1 cut(s) 1388
BaeI ACNNNNGTAYC 2 cut(s) 1941, 1974
BalI TGGCCA 1 cut(s) 1354
BanI GGYRCC 1 cut(s) 1291
BanII GRGCYC 3 cut(s) 150, 503, 595
BauI CACGAG 1 cut(s) 1389
BbsI GAAGAC 2 cut(s) 208, 1106
Bbv12I GWGCWC 4 cut(s) 595, 1388, 2031, 2268
BbvI GCAGC 5 cut(s) 68, 1812, 1840, 2320, 2342
BccI CCATC 4 cut(s) 116, 292, 1231, 1889
BceAI ACGGC 4 cut(s) 147, 175, 2000, 2496
BciT130I CCWGG 6 cut(s) 235, 282, 504, 759, 968, 984
BclI TGATCA 1 cut(s) 769
BcoDI GTCTC 8 cut(s) 905, 1211, 1784, 1863, 1988, 2177, 2484, 2546
BfaI CTAG 1 cut(s) 1322
BfmI CTRYAG 5 cut(s) 1128, 1713, 1826, 1944, 2481
BfuAI ACCTGC 1 cut(s) 1678
BglII AGATCT 1 cut(s) 1970
BisI GCNGC 8 cut(s) 57, 170, 303, 1262, 1826, 1829, 2331, 2334
BlsI GCNGC 8 cut(s) 58, 171, 304, 1263, 1827, 1830, 2332, 2335
BmcAI AGTACT 1 cut(s) 1041
Bme1390I CCNGG 6 cut(s) 235, 282, 504, 759, 968, 984
Bme18I GGWCC 4 cut(s) 129, 349, 448, 2000
BmeRI GACNNNNNGTC 1 cut(s) 2439
BmeT110I CYCGRG 1 cut(s) 61
BmgBI CACGTC 3 cut(s) 1112, 1628, 1680
BmgT120I GGNCC 6 cut(s) 129, 349, 448, 986, 2000, 2373
BmiI GGNNCC 3 cut(s) 130, 131, 1293
BmrFI CCNGG 6 cut(s) 235, 282, 504, 759, 968, 984
BmrI ACTGGG 1 cut(s) 608
BmsI GCATC 6 cut(s) 310, 1067, 1334, 1570, 1651, 2343
BmuI ACTGGG 1 cut(s) 608
BoxI GACNNNNGTC 1 cut(s) 162
BpiI GAAGAC 2 cut(s) 208, 1106
BpmI CTGGAG 2 cut(s) 989, 2447
BpuEI CTTGAG 4 cut(s) 464, 560, 881, 1590
Bsa29I ATCGAT 2 cut(s) 225, 459
BsaAI YACGTR 3 cut(s) 1234, 1586, 1886
BsaBI GATNNNNATC 1 cut(s) 807
BsaHI GRCGYC 2 cut(s) 172, 213
BsaI GGTCTC 2 cut(s) 905, 1211
BsaWI WCCGGW 1 cut(s) 2082
BsaXI ACNNNNNCTCC 2 cut(s) 543, 573
Bsc4I CCNNNNNNNGG 3 cut(s) 973, 2382, 2450
Bse1I ACTGG 7 cut(s) 399, 614, 1744, 1966, 2002, 2282, 2430
Bse3DI GCAATG 2 cut(s) 373, 1170
Bse8I GATNNNNATC 1 cut(s) 807
BseAI TCCGGA 1 cut(s) 2082
BseBI CCWGG 6 cut(s) 235, 282, 504, 759, 968, 984
BseCI ATCGAT 2 cut(s) 225, 459
BseGI GGATG 7 cut(s) 303, 325, 1090, 1246, 1666, 2344, 2395
BseJI GATNNNNATC 1 cut(s) 807
BseLI CCNNNNNNNGG 3 cut(s) 973, 2382, 2450
BseMI GCAATG 2 cut(s) 373, 1170
BseMII CTCAG 7 cut(s) 86, 282, 327, 955, 1110, 1110, 1623
BseNI ACTGG 7 cut(s) 399, 614, 1744, 1966, 2002, 2282, 2430
BseRI GAGGAG 2 cut(s) 750, 1217
BseSI GKGCMC 1 cut(s) 1388
BseX3I CGGCCG 1 cut(s) 160
BseXI GCAGC 5 cut(s) 68, 1812, 1840, 2320, 2342
BseYI CCCAGC 2 cut(s) 1246, 2032
BsgI GTGCAG 2 cut(s) 65, 2319
Bsh1285I CGRYCG 1 cut(s) 163
BshFI GGCC 5 cut(s) 162, 987, 1354, 2375, 2511
BshNI GGYRCC 1 cut(s) 1291
BshVI ATCGAT 2 cut(s) 225, 459
BsiEI CGRYCG 1 cut(s) 163
BsiHKAI GWGCWC 4 cut(s) 595, 1388, 2031, 2268
BsiHKCI CYCGRG 1 cut(s) 61
BsiSI CCGG 1 cut(s) 2083
BslFI GGGAC 4 cut(s) 115, 949, 1330, 2227
BslI CCNNNNNNNGG 3 cut(s) 973, 2382, 2450
BsmAI GTCTC 8 cut(s) 905, 1211, 1784, 1863, 1988, 2177, 2484, 2546
BsmFI GGGAC 4 cut(s) 115, 949, 1330, 2227
BsnI GGCC 5 cut(s) 162, 987, 1354, 2375, 2511
Bso31I GGTCTC 2 cut(s) 905, 1211
BsoBI CYCGRG 1 cut(s) 61
Bsp1286I GDGCHC 6 cut(s) 150, 503, 595, 1388, 2031, 2268
Bsp13I TCCGGA 1 cut(s) 2082
Bsp1407I TGTACA 2 cut(s) 619, 1006
BspANI GGCC 5 cut(s) 162, 987, 1354, 2375, 2511
BspCNI CTCAG 7 cut(s) 85, 283, 328, 954, 1109, 1111, 1624
BspDI ATCGAT 2 cut(s) 225, 459
BspEI TCCGGA 1 cut(s) 2082
BspLI GGNNCC 3 cut(s) 130, 131, 1293
BspMAI CTGCAG 2 cut(s) 1132, 1830
BspMI ACCTGC 1 cut(s) 1678
BspPI GGATC 4 cut(s) 1646, 1819, 1922, 2305
BspQI GCTCTTC 1 cut(s) 2449
BspT107I GGYRCC 1 cut(s) 1291
BspTNI GGTCTC 2 cut(s) 905, 1211
BsrDI GCAATG 2 cut(s) 373, 1170
BsrGI TGTACA 2 cut(s) 619, 1006
BsrI ACTGG 7 cut(s) 399, 614, 1744, 1966, 2002, 2282, 2430
BssNI GRCGYC 2 cut(s) 172, 213
BssSI CACGAG 1 cut(s) 1389
BssT1I CCWWGG 2 cut(s) 1925, 2044
Bst2BI CACGAG 1 cut(s) 1389
Bst2UI CCWGG 6 cut(s) 235, 282, 504, 759, 968, 984
Bst4CI ACNGT 9 cut(s) 44, 353, 963, 1620, 1957, 2168, 2197, 2441, 2450
Bst6I CTCTTC 5 cut(s) 339, 903, 1029, 1307, 2449
BstACI GRCGYC 2 cut(s) 172, 213
BstAUI TGTACA 2 cut(s) 619, 1006
BstBAI YACGTR 3 cut(s) 1234, 1586, 1886
BstC8I GCNNGC 3 cut(s) 89, 1559, 2461
BstEII GGTNACC 1 cut(s) 2431
BstF5I GGATG 7 cut(s) 303, 325, 1090, 1246, 1666, 2344, 2395
BstHHI GCGC 1 cut(s) 1807
BstMAI GTCTC 8 cut(s) 905, 1211, 1784, 1863, 1988, 2177, 2484, 2546
BstMCI CGRYCG 1 cut(s) 163
BstMWI GCNNNNNNNGC 2 cut(s) 88, 123
BstNI CCWGG 6 cut(s) 235, 282, 504, 759, 968, 984
BstNSI RCATGY 1 cut(s) 2463
BstPAI GACNNNNGTC 1 cut(s) 162
BstPI GGTNACC 1 cut(s) 2431
BstSCI CCNGG 6 cut(s) 233, 280, 502, 757, 966, 982
BstSFI CTRYAG 5 cut(s) 1128, 1713, 1826, 1944, 2481
BstSLI GKGCMC 1 cut(s) 1388
BstSNI TACGTA 1 cut(s) 1886
BstV1I GCAGC 5 cut(s) 68, 1812, 1840, 2320, 2342
BstV2I GAAGAC 2 cut(s) 208, 1106
BstX2I RGATCY 3 cut(s) 1811, 1914, 1970
BstYI RGATCY 3 cut(s) 1811, 1914, 1970
BstZI CGGCCG 1 cut(s) 160
Bsu15I ATCGAT 2 cut(s) 225, 459
BsuRI GGCC 5 cut(s) 162, 987, 1354, 2375, 2511
BsuTUI ATCGAT 2 cut(s) 225, 459
BtrI CACGTC 3 cut(s) 1112, 1628, 1680
BtsCI GGATG 7 cut(s) 303, 325, 1090, 1246, 1666, 2344, 2395
BtsI GCAGTG 2 cut(s) 77, 924
BtsIMutI CAGTG 7 cut(s) 77, 798, 924, 1104, 1593, 1647, 1959
BveI ACCTGC 1 cut(s) 1678
Cac8I GCNNGC 3 cut(s) 89, 1559, 2461
CaiI CAGNNNCTG 2 cut(s) 79, 967
CfoI GCGC 1 cut(s) 1807
Cfr13I GGNCC 6 cut(s) 129, 349, 448, 986, 2000, 2373
ClaI ATCGAT 2 cut(s) 225, 459
CseI GACGC 2 cut(s) 161, 263
DriI GACNNNNNGTC 1 cut(s) 2439
EaeI YGGCCR 2 cut(s) 160, 1352
EagI CGGCCG 1 cut(s) 160
Eam1104I CTCTTC 5 cut(s) 339, 903, 1029, 1307, 2449
Eam1105I GACNNNNNGTC 1 cut(s) 2439
EarI CTCTTC 5 cut(s) 339, 903, 1029, 1307, 2449
EciI GGCGGA 1 cut(s) 233
Ecl136II GAGCTC 1 cut(s) 593
EclXI CGGCCG 1 cut(s) 160
Eco105I TACGTA 1 cut(s) 1886
Eco130I CCWWGG 2 cut(s) 1925, 2044
Eco24I GRGCYC 3 cut(s) 150, 503, 595
Eco31I GGTCTC 2 cut(s) 905, 1211
Eco32I GATATC 1 cut(s) 2062
Eco47I GGWCC 4 cut(s) 129, 349, 448, 2000
Eco52I CGGCCG 1 cut(s) 160
Eco53kI GAGCTC 1 cut(s) 593
Eco57I CTGAAG 2 cut(s) 432, 1088
Eco88I CYCGRG 1 cut(s) 61
Eco91I GGTNACC 1 cut(s) 2431
EcoICRI GAGCTC 1 cut(s) 593
EcoO109I RGGNCCY 2 cut(s) 129, 448
EcoO65I GGTNACC 1 cut(s) 2431
EcoRI GAATTC 1 cut(s) 331
EcoRII CCWGG 6 cut(s) 233, 280, 502, 757, 966, 982
EcoRV GATATC 1 cut(s) 2062
EcoT14I CCWWGG 2 cut(s) 1925, 2044
EcoT22I ATGCAT 2 cut(s) 1060, 2358
EcoT38I GRGCYC 3 cut(s) 150, 503, 595
ErhI CCWWGG 2 cut(s) 1925, 2044
FaqI GGGAC 4 cut(s) 115, 949, 1330, 2227
FauI CCCGC 1 cut(s) 119
FauNDI CATATG 1 cut(s) 1673
FbaI TGATCA 1 cut(s) 769
FblI GTMKAC 1 cut(s) 491
Fnu4HI GCNGC 8 cut(s) 57, 170, 303, 1262, 1826, 1829, 2331, 2334
FokI GGATG 7 cut(s) 310, 332, 1097, 1253, 1673, 2331, 2402
FriOI GRGCYC 3 cut(s) 150, 503, 595
Fsp4HI GCNGC 8 cut(s) 57, 170, 303, 1262, 1826, 1829, 2331, 2334
FspBI CTAG 1 cut(s) 1322
GlaI GCGC 1 cut(s) 1806
GluI GCNGC 8 cut(s) 57, 170, 303, 1262, 1826, 1829, 2331, 2334
GsaI CCCAGC 2 cut(s) 1250, 2036
GsuI CTGGAG 2 cut(s) 989, 2447
HaeIII GGCC 5 cut(s) 162, 987, 1354, 2375, 2511
HapII CCGG 1 cut(s) 2083
HgaI GACGC 2 cut(s) 161, 263
HhaI GCGC 1 cut(s) 1807
Hin1I GRCGYC 2 cut(s) 172, 213
Hin6I GCGC 1 cut(s) 1805
HinP1I GCGC 1 cut(s) 1805
HincII GTYRAC 3 cut(s) 1093, 1623, 2275
HindII GTYRAC 3 cut(s) 1093, 1623, 2275
HindIII AAGCTT 2 cut(s) 101, 536
HpaI GTTAAC 1 cut(s) 2275
HpaII CCGG 1 cut(s) 2083
HphI GGTGA 6 cut(s) 644, 722, 935, 1636, 1883, 2443
Hpy99I CGWCG 3 cut(s) 215, 257, 1217
HpyAV CCTTC 4 cut(s) 963, 1651, 1702, 1795
HpyCH4III ACNGT 9 cut(s) 44, 353, 963, 1620, 1957, 2168, 2197, 2441, 2450
HpyF10VI GCNNNNNNNGC 2 cut(s) 88, 123
Hsp92I GRCGYC 2 cut(s) 172, 213
HspAI GCGC 1 cut(s) 1805
KflI GGGWCCC 1 cut(s) 129
Kpn2I TCCGGA 1 cut(s) 2082
Ksp22I TGATCA 1 cut(s) 769
KspAI GTTAAC 1 cut(s) 2275
LguI GCTCTTC 1 cut(s) 2449
LmnI GCTCC 5 cut(s) 378, 439, 762, 1500, 2263
Lsp1109I GCAGC 5 cut(s) 68, 1812, 1840, 2320, 2342
LweI GCATC 6 cut(s) 310, 1067, 1334, 1570, 1651, 2343
MaeI CTAG 1 cut(s) 1322
MflI RGATCY 3 cut(s) 1811, 1914, 1970
MhlI GDGCHC 6 cut(s) 150, 503, 595, 1388, 2031, 2268
MlsI TGGCCA 1 cut(s) 1354
MluNI TGGCCA 1 cut(s) 1354
MlyI GAGTC 5 cut(s) 185, 347, 1088, 1431, 2179
MmeI TCCRAC 8 cut(s) 213, 234, 276, 417, 1515, 1571, 2262, 2467
Mox20I TGGCCA 1 cut(s) 1354
Mph1103I ATGCAT 2 cut(s) 1060, 2358
MroI TCCGGA 1 cut(s) 2082
MroXI GAANNNNTTC 2 cut(s) 1023, 2106
MscI TGGCCA 1 cut(s) 1354
MseI TTAA 7 cut(s) 269, 1178, 1520, 1902, 1919, 2274, 2301
MslI CAYNNNNRTG 2 cut(s) 1269, 1566
Msp20I TGGCCA 1 cut(s) 1354
MspA1I CMGCKG 5 cut(s) 91, 169, 1331, 1498, 2333
MspI CCGG 1 cut(s) 2083
MspR9I CCNGG 6 cut(s) 235, 282, 504, 759, 968, 984
MvaI CCWGG 6 cut(s) 235, 282, 504, 759, 968, 984
MwoI GCNNNNNNNGC 2 cut(s) 88, 123
NdeI CATATG 1 cut(s) 1673
NlaIV GGNNCC 3 cut(s) 130, 131, 1293
NmeAIII GCCGAG 1 cut(s) 1556
NmuCI GTSAC 7 cut(s) 75, 796, 923, 1871, 1957, 2431, 2513
NsiI ATGCAT 2 cut(s) 1060, 2358
NspI RCATGY 1 cut(s) 2463
PaeI GCATGC 1 cut(s) 2463
PciSI GCTCTTC 1 cut(s) 2449
PcsI WCGNNNNNNNCGW 2 cut(s) 18, 150
PdmI GAANNNNTTC 2 cut(s) 1023, 2106
PfeI GAWTC 6 cut(s) 9, 670, 886, 1019, 1538, 2221
PflFI GACNNNGTC 1 cut(s) 29
PkrI GCNGC 8 cut(s) 58, 171, 304, 1263, 1827, 1830, 2332, 2335
PleI GAGTC 5 cut(s) 185, 346, 1088, 1431, 2179
PpsI GAGTC 5 cut(s) 185, 346, 1088, 1431, 2179
Ppu21I YACGTR 3 cut(s) 1234, 1586, 1886
PpuMI RGGWCCY 2 cut(s) 129, 448
PshAI GACNNNNGTC 1 cut(s) 162
PsiI TTATAA 1 cut(s) 1157
Psp124BI GAGCTC 1 cut(s) 595
Psp1406I AACGTT 1 cut(s) 1426
Psp5II RGGWCCY 2 cut(s) 129, 448
Psp6I CCWGG 6 cut(s) 233, 280, 502, 757, 966, 982
PspEI GGTNACC 1 cut(s) 2431
PspFI CCCAGC 2 cut(s) 1246, 2032
PspGI CCWGG 6 cut(s) 233, 280, 502, 757, 966, 982
PspN4I GGNNCC 3 cut(s) 130, 131, 1293
PspPI GGNCC 6 cut(s) 129, 349, 448, 986, 2000, 2373
PspPPI RGGWCCY 2 cut(s) 129, 448
PsrI GAACNNNNNNTAC 2 cut(s) 1933, 1965
PstI CTGCAG 2 cut(s) 1132, 1830
PstNI CAGNNNCTG 2 cut(s) 79, 967
PsuI RGATCY 3 cut(s) 1811, 1914, 1970
PsyI GACNNNGTC 1 cut(s) 29
PvuII CAGCTG 3 cut(s) 91, 1331, 2333
RseI CAYNNNNRTG 2 cut(s) 1269, 1566
SacI GAGCTC 1 cut(s) 595
SapI GCTCTTC 1 cut(s) 2449
SaqAI TTAA 7 cut(s) 269, 1178, 1520, 1902, 1919, 2274, 2301
SatI GCNGC 8 cut(s) 57, 170, 303, 1262, 1826, 1829, 2331, 2334
Sau96I GGNCC 6 cut(s) 129, 349, 448, 986, 2000, 2373
ScaI AGTACT 1 cut(s) 1041
SchI GAGTC 5 cut(s) 185, 347, 1088, 1431, 2179
ScrFI CCNGG 6 cut(s) 235, 282, 504, 759, 968, 984
SduI GDGCHC 6 cut(s) 150, 503, 595, 1388, 2031, 2268
SfaNI GCATC 6 cut(s) 310, 1067, 1334, 1570, 1651, 2343
SfcI CTRYAG 5 cut(s) 1128, 1713, 1826, 1944, 2481
SinI GGWCC 4 cut(s) 129, 349, 448, 2000
SmiMI CAYNNNNRTG 2 cut(s) 1269, 1566
SmlI CTYRAG 4 cut(s) 443, 539, 860, 1605
SmoI CTYRAG 4 cut(s) 443, 539, 860, 1605
SnaBI TACGTA 1 cut(s) 1886
SphI GCATGC 1 cut(s) 2463
SspI AATATT 4 cut(s) 1033, 1068, 2106, 2150
SspMI CTAG 1 cut(s) 1322
SstI GAGCTC 1 cut(s) 595
StyD4I CCNGG 6 cut(s) 233, 280, 502, 757, 966, 982
StyI CCWWGG 2 cut(s) 1925, 2044
TaaI ACNGT 9 cut(s) 44, 353, 963, 1620, 1957, 2168, 2197, 2441, 2450
TatI WGTACW 6 cut(s) 619, 1006, 1039, 1225, 1734, 2341
TauI GCSGC 3 cut(s) 172, 305, 1264
TfiI GAWTC 6 cut(s) 9, 670, 886, 1019, 1538, 2221
Tru1I TTAA 7 cut(s) 269, 1178, 1520, 1902, 1919, 2274, 2301
Tru9I TTAA 7 cut(s) 269, 1178, 1520, 1902, 1919, 2274, 2301
TscAI CASTG 7 cut(s) 84, 805, 924, 1104, 1600, 1654, 1966
TseFI GTSAC 7 cut(s) 75, 796, 923, 1871, 1957, 2431, 2513
TseI GCWGC 5 cut(s) 56, 1825, 1828, 2330, 2333
Tsp45I GTSAC 7 cut(s) 75, 796, 923, 1871, 1957, 2431, 2513
TspGWI ACGGA 1 cut(s) 391
TspRI CASTG 7 cut(s) 84, 805, 924, 1104, 1600, 1654, 1966
Tth111I GACNNNGTC 1 cut(s) 29
VneI GTGCAC 1 cut(s) 1384
VpaK11BI GGWCC 4 cut(s) 129, 349, 448, 2000
XapI RAATTY 8 cut(s) 331, 946, 1011, 1279, 1516, 2054, 2240, 2252
XceI RCATGY 1 cut(s) 2463
XcmI CCANNNNNNNNNTGG 1 cut(s) 2383
XmiI GTMKAC 1 cut(s) 491
XmnI GAANNNNTTC 2 cut(s) 1023, 2106
XspI CTAG 1 cut(s) 1322
ZraI GACGTC 1 cut(s) 214
ZrmI AGTACT 1 cut(s) 1041
Zsp2I ATGCAT 2 cut(s) 1060, 2358
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.