Rh2AG095700

Tubulin--tyrosine ligase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0016100
Physical Location & Seq
Forward (+)
8056717 .. 8061131
4415 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG095700.1

Sequence Viewer

Length: 2589 bp
ATGTCAGCACCGGCAAAAAGAATTGAAACCTACGAAGACTTCGTCGAAGTTCACGGCTTACTTCTGGCCGCCTCGGGATTGCCCCAGTCCCTGCACCGCCAGCTCTTCCAGAAGCTTCTCTCCGACACCTTCGACGGCGGGTCCCACTTCCAAATCGAGCCCACCTCCGACGGCCACCAGCGCCGCCTCGTACTCACCTCCGGCTCCATACCCCAAAACTCCGACGTCTTCCTCGTTGACCACGCCTGGACCTTCCGCCTCTCCGAAGCTTCCAAACAGTTAAGGGAAGTTCCGGGATTGGCTGAGAGAATGGCGAGCATAATGTGTGTGGATATTGATTTGAATTCAGAAGAGAACCAAGTTCTGAGTGAGGGTGATGCAAAACGCAGCGTTTTGGACGTACTGGAAAGTGAGATTAGTGAAGCCAATGGGAATGTCAAGTGGTTGGAGCTTGAAGAACTTGGCATTGACAATGAAACGCTGTCGTCTTTGGACTTGTCTGCTAAGTGCCCTGGTTTGATTGCATTGTCCTTGTGTGGGAATAAGATTGACAATGTGGAGGTAGTTGTTGCGGAAGTTAACAAATTGAAAAACCTAAGAGCGCTTTGGCTGAACAACAACCAGGTTGTAGAAAATCGTGGCAATGAATTGGGGGATAGGATTCTTCAAGAATTGCCGAATCTTGAAATCTATAACTCAAAGTTTACCGCCAATTTCGGTGAGTGGGCACTGGGGTTTTGTGGAGGAGTGTTTGACAAGGAAAATCCGGGCAGCATTGATCTGCCTGACCGTCCATTGCAGCAAGTCACGGATCTTGATCTTTCAGATAGATGTATTCAGAGTTTAATCAATAAGGCATTTACCCCTTTTCAGTTGCCATGTCTTTCATACTTGAATCTTCGGGGAAATCCTTTAGAAGAGACCTCACTGGCCGACTTGTTATATGTACTACGGAATTTTCCTTCATTACAGTCTCTGGAGGTGGATATTCCTGGCCCTCTTGGAGAAAGTGCTCTAGAAATTCTTGAATCTCTTCCTAATCTTTCTGTACTGAATGGTGTCAATGCATCAAAGATATTTGAAACTGAAAAGCATGTGGTTGACTCAGCGCTTCAGCCACGTCTTCCTGAGTGGACTCCTGATGAGCCTCTTCCTGATCGTGTTATAAGTGCAATGTGGCAATATCTAATGACATATAGACTTGCGGACGAAGAGAAGATAGACGAGACCTCTGTGTTGTACGTGATGGATGAGCTAGGCTCAGCTTTGAGGCATAGTGATGAGCCAAATTTCAGAGTATCTCCTTTTCTGTTTATGCCAGAGGGGACATTGGCATCAGCTGTGAGTTTCTGCATATTATGGCCAATCAAGAATGTTGAAAAAGGTTATGAATGCACTCGTGATTATCTTTTTGGTATTGGGGAGGACAAGCAACGTTCTGCCAGACTTACAGCTTGGTTCCATACACCAGAGAAGTATTTTATTCGAGAGTATGAGAAGCACTGGCAGAACTTGCGAGCAAAAAGTTTAACTTCTCCTCTGATGGAGTCATGTCCAACTAAAAGTTTAAATCACAATGAAAAACGCGCTTTACGTGTGTACTCCGATATCCCTCAAGTTGAAGAACTTTTGACACGTCTGGAGTTTGTATTCACACCTGATCCAAAGGATGCAGAAATTATATGGACAGGTGACCAAGTGGATGAGGATATGAAGAAAGCTTGGGGAATAACAGACAATCAGTTCATAAACCAGTTTCCTTTTGAGGCTTGTATAGTCATGAAACATCATCTGGCAGATACCATTCAGAAGGCACTTGGATCTCCTGAGTGGATGCAGCCAACCTATAATCTGGAAACACATCTATCCCAACTTATTGGTGACTATTGTGTACGTAAAAGAGATGGGCTTAACAATCTGTGGATCTTAAAACCTTGGAACATGGCACGAACTATAGATACAACTGTCATTGATAATTTATCTGCTATTATTCGGCTCATGGAGACTGGACCGAAGATATGTCAGAAGTATATTGAGCACCCAGCCTTGTTCAAAGGGAGAAAGTTTGATATCCGCTACATAGTTTTACTACGGAGTTTGAACCCTATGGAAATATTTCTGTCAGACACTTTCTGGGTTAGACTGGCAAACAACCAATATTCTTTAGAAAAGCACAGTTTTTTTGAGTATGAGACTCACTTTACTGTTATGAATTATCGAGGGACACTGAATCACATGAACACGTCGGAATTTGTAAGAGAGTTCGAACAGGAGCACCAAGTTAAATGGTCCGATATTCACTTTAGAGTCAAAAAAATGATCCGATCAGTCTTTGAGGCAGCTGCAAAAGTGCACCCTGAGATGCACAGTCCAACGTCGAGGGCTATGTATGGTGTGGATGTCATGCTTGATACTTCTTTCCAGCCAAAGTTACTGGAGGTGACTTACTGTCCTGACTGTACAAGAGCATGCAAGTATGATATGGAGGCAATAGTCGCAGGAGGGGAAGTGGTTAGAGCTCGTGAATTTTATAACTATGTCTTTGGTTGTCTCTTTTTGAATGAAGCCACTCATGTTAGTCCATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TTL

Protein Analysis

862

Amino Acids

98.54

Weight (kDa)

5.05

Isoelectric Point (pI)

37.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SET_TTL PF25556 72 - 120 4e-12 Tubulin tyrosine ligase SET-like domain
SET_TTL PF25556 380 - 488 4.6e-22 Tubulin tyrosine ligase SET-like domain
TTL PF03133 580 - 830 5e-51 Tubulin-tyrosine ligase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016100)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G77550
fragaria_vesca FvH4_1g08650 FvH4_1g08650
malus_domestica MD15G1217600.v1.1
prunus_persica Prupe.7G198800_v2.0.a1
pyrus_communis pycom15g19320
rosa_chinensis RchiOBHm_Chr2g0094931
rosa_laevigata RLG00000016532
rosa_multiflora Rmu_sc0004316.1_g000014
rosa_roxburghii Rroxscaffold_2G00146760
rosa_rugosa Rorug02G0048900
rosa_samantha Rh2AG095700 Rh2CG098600 Rh2DG095300
rosa_wichuraiana Rw2G007200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1166, 2532
AatII GACGTC 1 cut(s) 228
AccII CGCG 1 cut(s) 1587
AciI CCGC 9 cut(s) 69, 97, 138, 184, 256, 572, 708, 1205, 2074
AclI AACGTT 1 cut(s) 1435
AclWI GGATC 5 cut(s) 819, 1655, 1828, 1931, 2314
AcoI YGGCCR 4 cut(s) 66, 172, 930, 1361
AcsI RAATTY 6 cut(s) 343, 955, 1020, 1288, 2249, 2525
AcuI CTGAAG 1 cut(s) 1097
AcyI GRCGYC 1 cut(s) 225
AfaI GTAC 8 cut(s) 192, 402, 948, 1050, 1241, 1601, 1893, 2461
AfeI AGCGCT 2 cut(s) 603, 1110
AfiI CCNNNNNNNGG 1 cut(s) 537
AflIII ACRYGT 3 cut(s) 1594, 1634, 2241
AhdI GACNNNNNGTC 2 cut(s) 139, 2448
AjiI CACGTC 3 cut(s) 1121, 1637, 2244
AjnI CCWGG 4 cut(s) 245, 511, 621, 991
Alw21I GWGCWC 5 cut(s) 1015, 2040, 2277, 2355, 2521
Alw26I GTCTC 6 cut(s) 914, 978, 1220, 1997, 2186, 2555
Alw44I GTGCAC 1 cut(s) 2351
AlwI GGATC 5 cut(s) 819, 1655, 1828, 1931, 2314
AlwNI CAGNNNCTG 2 cut(s) 91, 976
Ama87I CYCGRG 1 cut(s) 73
Aor51HI AGCGCT 2 cut(s) 603, 1110
AoxI GGCC 5 cut(s) 66, 172, 930, 994, 1361
ApaLI GTGCAC 1 cut(s) 2351
ApeKI GCWGC 6 cut(s) 387, 771, 799, 1837, 2339, 2342
ApoI RAATTY 6 cut(s) 343, 955, 1020, 1288, 2249, 2525
ArsI GACNNNNNNTTYG 2 cut(s) 27, 59
Asp700I GAANNNNTTC 2 cut(s) 1032, 2115
AspLEI GCGC 4 cut(s) 183, 604, 1111, 1589
AspS9I GGNCC 5 cut(s) 141, 249, 995, 2009, 2289
AsuC2I CCSGG 2 cut(s) 294, 768
AsuHPI GGTGA 6 cut(s) 187, 386, 731, 1703, 1892, 2452
AsuII TTCGAA 1 cut(s) 2265
AvaI CYCGRG 1 cut(s) 73
AvaII GGWCC 4 cut(s) 141, 249, 2009, 2289
BaeGI GKGCMC 3 cut(s) 512, 730, 2355
BaeI ACNNNNGTAYC 2 cut(s) 1950, 1983
BalI TGGCCA 1 cut(s) 1363
BanII GRGCYC 2 cut(s) 162, 2521
BauI CACGAG 2 cut(s) 1398, 2520
BbsI GAAGAC 3 cut(s) 42, 220, 1115
Bbv12I GWGCWC 5 cut(s) 1015, 2040, 2277, 2355, 2521
BbvI GCAGC 6 cut(s) 399, 783, 811, 1849, 2329, 2351
BccI CCATC 3 cut(s) 1240, 1537, 1898
BceAI ACGGC 3 cut(s) 70, 151, 187
BciT130I CCWGG 4 cut(s) 247, 513, 623, 993
BcnI CCSGG 2 cut(s) 294, 768
BcoDI GTCTC 6 cut(s) 914, 978, 1220, 1997, 2186, 2555
BfaI CTAG 2 cut(s) 1016, 1256
BfmI CTRYAG 1 cut(s) 1953
BfoI RGCGCY 3 cut(s) 184, 605, 1112
BisI GCNGC 8 cut(s) 69, 184, 388, 772, 800, 1838, 2340, 2343
BlpI GCTNAGC 1 cut(s) 1261
BlsI GCNGC 8 cut(s) 70, 185, 389, 773, 801, 1839, 2341, 2344
Bme1390I CCNGG 6 cut(s) 247, 294, 513, 623, 768, 993
Bme18I GGWCC 4 cut(s) 141, 249, 2009, 2289
BmeRI GACNNNNNGTC 2 cut(s) 139, 2448
BmeT110I CYCGRG 1 cut(s) 73
BmgBI CACGTC 3 cut(s) 1121, 1637, 2244
BmgT120I GGNCC 5 cut(s) 141, 249, 995, 2009, 2289
BmiI GGNNCC 4 cut(s) 142, 143, 205, 1460
BmrFI CCNGG 6 cut(s) 247, 294, 513, 623, 768, 993
BmrI ACTGGG 2 cut(s) 79, 740
BmsI GCATC 6 cut(s) 367, 1076, 1343, 1660, 1824, 2352
BmuI ACTGGG 2 cut(s) 79, 740
BpiI GAAGAC 3 cut(s) 42, 220, 1115
BplI GAGNNNNNCTC 4 cut(s) 149, 181, 1244, 1276
BpmI CTGGAG 3 cut(s) 998, 1661, 2456
Bpu1102I GCTNAGC 1 cut(s) 1261
Bpu14I TTCGAA 1 cut(s) 2265
BpuEI CTTGAG 1 cut(s) 1599
BpuMI CCSGG 2 cut(s) 294, 768
BsaAI YACGTR 3 cut(s) 1243, 1595, 1895
BsaBI GATNNNNATC 1 cut(s) 816
BsaHI GRCGYC 1 cut(s) 225
BsaI GGTCTC 2 cut(s) 914, 1220
BsaJI CCNNGG 3 cut(s) 72, 511, 1934
BsaXI ACNNNNNCTCC 2 cut(s) 2493, 2523
Bsc4I CCNNNNNNNGG 1 cut(s) 537
Bse118I RCCGGY 1 cut(s) 10
Bse1I ACTGG 9 cut(s) 85, 408, 735, 933, 1508, 1753, 2011, 2148, 2439
Bse3DI GCAATG 3 cut(s) 649, 794, 1179
Bse8I GATNNNNATC 1 cut(s) 816
BseBI CCWGG 4 cut(s) 247, 513, 623, 993
BseDI CCNNGG 3 cut(s) 72, 511, 1934
BseGI GGATG 5 cut(s) 1255, 1675, 1708, 1839, 2404
BseJI GATNNNNATC 1 cut(s) 816
BseLI CCNNNNNNNGG 1 cut(s) 537
BseMI GCAATG 3 cut(s) 649, 794, 1179
BseMII CTCAG 7 cut(s) 294, 356, 1119, 1119, 1275, 1818, 2349
BseNI ACTGG 9 cut(s) 85, 408, 735, 933, 1508, 1753, 2011, 2148, 2439
BseRI GAGGAG 2 cut(s) 759, 1527
BseSI GKGCMC 3 cut(s) 512, 730, 2355
BseXI GCAGC 6 cut(s) 399, 783, 811, 1849, 2329, 2351
BseYI CCCAGC 1 cut(s) 2041
BsgI GTGCAG 1 cut(s) 77
Bsh1236I CGCG 1 cut(s) 1587
BshFI GGCC 5 cut(s) 68, 174, 932, 996, 1363
BsiHKAI GWGCWC 5 cut(s) 1015, 2040, 2277, 2355, 2521
BsiHKCI CYCGRG 1 cut(s) 73
BsiSI CCGG 4 cut(s) 11, 201, 293, 767
BslFI GGGAC 4 cut(s) 73, 127, 1339, 2236
BslI CCNNNNNNNGG 1 cut(s) 537
BsmAI GTCTC 6 cut(s) 914, 978, 1220, 1997, 2186, 2555
BsmFI GGGAC 4 cut(s) 73, 127, 1339, 2236
BsmI GAATGC 1 cut(s) 1397
BsnI GGCC 5 cut(s) 68, 174, 932, 996, 1363
Bso31I GGTCTC 2 cut(s) 914, 1220
BsoBI CYCGRG 1 cut(s) 73
Bsp119I TTCGAA 1 cut(s) 2265
Bsp1286I GDGCHC 8 cut(s) 162, 512, 730, 1015, 2040, 2277, 2355, 2521
Bsp1407I TGTACA 1 cut(s) 2459
Bsp143I GATC 9 cut(s) 778, 811, 817, 1156, 1660, 1820, 1923, 2319, 2324
Bsp1720I GCTNAGC 1 cut(s) 1261
BspACI CCGC 9 cut(s) 69, 97, 138, 184, 256, 572, 708, 1205, 2074
BspANI GGCC 5 cut(s) 68, 174, 932, 996, 1363
BspCNI CTCAG 7 cut(s) 295, 357, 1118, 1120, 1274, 1819, 2350
BspFNI CGCG 1 cut(s) 1587
BspHI TCATGA 1 cut(s) 1779
BspLI GGNNCC 4 cut(s) 142, 143, 205, 1460
BspPI GGATC 5 cut(s) 819, 1655, 1828, 1931, 2314
BspQI GCTCTTC 1 cut(s) 110
BspT104I TTCGAA 1 cut(s) 2265
BspTNI GGTCTC 2 cut(s) 914, 1220
BsrDI GCAATG 3 cut(s) 649, 794, 1179
BsrFI RCCGGY 1 cut(s) 10
BsrGI TGTACA 1 cut(s) 2459
BsrI ACTGG 9 cut(s) 85, 408, 735, 933, 1508, 1753, 2011, 2148, 2439
BssAI RCCGGY 1 cut(s) 10
BssECI CCNNGG 3 cut(s) 72, 511, 1934
BssMI GATC 9 cut(s) 778, 811, 817, 1156, 1660, 1820, 1923, 2319, 2324
BssNI GRCGYC 1 cut(s) 225
BssSI CACGAG 2 cut(s) 1398, 2520
BssT1I CCWWGG 1 cut(s) 1934
Bst2BI CACGAG 2 cut(s) 1398, 2520
Bst2UI CCWGG 4 cut(s) 247, 513, 623, 993
Bst4CI ACNGT 9 cut(s) 279, 791, 972, 1966, 2177, 2206, 2369, 2450, 2459
Bst6I CTCTTC 6 cut(s) 110, 345, 912, 1038, 1155, 1206
BstACI GRCGYC 1 cut(s) 225
BstAPI GCANNNNNTGC 1 cut(s) 1513
BstAUI TGTACA 1 cut(s) 2459
BstBAI YACGTR 3 cut(s) 1243, 1595, 1895
BstBI TTCGAA 1 cut(s) 2265
BstC8I GCNNGC 4 cut(s) 101, 316, 1518, 2470
BstDEI CTNAG 9 cut(s) 303, 365, 504, 596, 1105, 1128, 1261, 1827, 2358
BstEII GGTNACC 1 cut(s) 1691
BstF5I GGATG 5 cut(s) 1255, 1675, 1708, 1839, 2404
BstFNI CGCG 1 cut(s) 1587
BstH2I RGCGCY 3 cut(s) 184, 605, 1112
BstHHI GCGC 4 cut(s) 183, 604, 1111, 1589
BstKTI GATC 9 cut(s) 781, 814, 820, 1159, 1663, 1823, 1926, 2322, 2327
BstMAI GTCTC 6 cut(s) 914, 978, 1220, 1997, 2186, 2555
BstMBI GATC 9 cut(s) 778, 811, 817, 1156, 1660, 1820, 1923, 2319, 2324
BstMWI GCNNNNNNNGC 4 cut(s) 100, 180, 1513, 2495
BstNI CCWGG 4 cut(s) 247, 513, 623, 993
BstNSI RCATGY 2 cut(s) 1097, 2472
BstPI GGTNACC 1 cut(s) 1691
BstSCI CCNGG 6 cut(s) 245, 292, 511, 621, 766, 991
BstSFI CTRYAG 1 cut(s) 1953
BstSLI GKGCMC 3 cut(s) 512, 730, 2355
BstSNI TACGTA 1 cut(s) 1895
BstUI CGCG 1 cut(s) 1587
BstV1I GCAGC 6 cut(s) 399, 783, 811, 1849, 2329, 2351
BstV2I GAAGAC 3 cut(s) 42, 220, 1115
BstX2I RGATCY 3 cut(s) 811, 1820, 1923
BstXI CCANNNNNNTGG 1 cut(s) 1877
BstYI RGATCY 3 cut(s) 811, 1820, 1923
BsuRI GGCC 5 cut(s) 68, 174, 932, 996, 1363
BtrI CACGTC 3 cut(s) 1121, 1637, 2244
BtsCI GGATG 5 cut(s) 1255, 1675, 1708, 1839, 2404
BtsIMutI CAGTG 4 cut(s) 728, 926, 1501, 2225
Cac8I GCNNGC 4 cut(s) 101, 316, 1518, 2470
CaiI CAGNNNCTG 2 cut(s) 91, 976
CciI TCATGA 1 cut(s) 1779
CfoI GCGC 4 cut(s) 183, 604, 1111, 1589
Cfr10I RCCGGY 1 cut(s) 10
Cfr13I GGNCC 5 cut(s) 141, 249, 995, 2009, 2289
CsiI ACCWGGT 1 cut(s) 621
Csp6I GTAC 8 cut(s) 191, 401, 947, 1049, 1240, 1600, 1892, 2460
CviQI GTAC 8 cut(s) 191, 401, 947, 1049, 1240, 1600, 1892, 2460
DdeI CTNAG 9 cut(s) 303, 365, 504, 596, 1105, 1128, 1261, 1827, 2358
DpnI GATC 9 cut(s) 780, 813, 819, 1158, 1662, 1822, 1925, 2321, 2326
DpnII GATC 9 cut(s) 778, 811, 817, 1156, 1660, 1820, 1923, 2319, 2324
DraI TTTAAA 1 cut(s) 1569
DriI GACNNNNNGTC 2 cut(s) 139, 2448
EaeI YGGCCR 4 cut(s) 66, 172, 930, 1361
Eam1104I CTCTTC 6 cut(s) 110, 345, 912, 1038, 1155, 1206
Eam1105I GACNNNNNGTC 2 cut(s) 139, 2448
EarI CTCTTC 6 cut(s) 110, 345, 912, 1038, 1155, 1206
EciI GGCGGA 1 cut(s) 245
Ecl136II GAGCTC 1 cut(s) 2519
Eco105I TACGTA 1 cut(s) 1895
Eco130I CCWWGG 1 cut(s) 1934
Eco24I GRGCYC 2 cut(s) 162, 2521
Eco31I GGTCTC 2 cut(s) 914, 1220
Eco32I GATATC 2 cut(s) 1609, 2071
Eco47I GGWCC 4 cut(s) 141, 249, 2009, 2289
Eco47III AGCGCT 2 cut(s) 603, 1110
Eco53kI GAGCTC 1 cut(s) 2519
Eco57I CTGAAG 1 cut(s) 1097
Eco88I CYCGRG 1 cut(s) 73
Eco91I GGTNACC 1 cut(s) 1691
EcoICRI GAGCTC 1 cut(s) 2519
EcoO109I RGGNCCY 1 cut(s) 141
EcoO65I GGTNACC 1 cut(s) 1691
EcoRI GAATTC 1 cut(s) 343
EcoRII CCWGG 4 cut(s) 245, 511, 621, 991
EcoRV GATATC 2 cut(s) 1609, 2071
EcoT14I CCWWGG 1 cut(s) 1934
EcoT22I ATGCAT 1 cut(s) 1069
EcoT38I GRGCYC 2 cut(s) 162, 2521
ErhI CCWWGG 1 cut(s) 1934
FalI AAGNNNNNCTT 2 cut(s) 1516, 1548
FaqI GGGAC 4 cut(s) 73, 127, 1339, 2236
FauI CCCGC 1 cut(s) 131
Fnu4HI GCNGC 8 cut(s) 69, 184, 388, 772, 800, 1838, 2340, 2343
FokI GGATG 5 cut(s) 1262, 1682, 1715, 1846, 2411
FriOI GRGCYC 2 cut(s) 162, 2521
Fsp4HI GCNGC 8 cut(s) 69, 184, 388, 772, 800, 1838, 2340, 2343
FspBI CTAG 2 cut(s) 1016, 1256
GlaI GCGC 4 cut(s) 182, 603, 1110, 1588
GluI GCNGC 8 cut(s) 69, 184, 388, 772, 800, 1838, 2340, 2343
GsaI CCCAGC 1 cut(s) 2045
GsuI CTGGAG 3 cut(s) 998, 1661, 2456
HaeII RGCGCY 3 cut(s) 184, 605, 1112
HaeIII GGCC 5 cut(s) 68, 174, 932, 996, 1363
HapII CCGG 4 cut(s) 11, 201, 293, 767
HhaI GCGC 4 cut(s) 183, 604, 1111, 1589
Hin1I GRCGYC 1 cut(s) 225
Hin6I GCGC 4 cut(s) 181, 602, 1109, 1587
HinP1I GCGC 4 cut(s) 181, 602, 1109, 1587
HincII GTYRAC 3 cut(s) 238, 580, 1102
HindII GTYRAC 3 cut(s) 238, 580, 1102
HindIII AAGCTT 3 cut(s) 113, 267, 1719
HpaI GTTAAC 1 cut(s) 580
HpaII CCGG 4 cut(s) 11, 201, 293, 767
HphI GGTGA 6 cut(s) 187, 386, 731, 1703, 1892, 2452
Hpy166II GTNNAC 9 cut(s) 52, 238, 580, 705, 1102, 1134, 1600, 1892, 2353
Hpy8I GTNNAC 9 cut(s) 52, 238, 580, 705, 1102, 1134, 1600, 1892, 2353
Hpy99I CGWCG 6 cut(s) 47, 137, 173, 227, 2248, 2380
HpyAV CCTTC 4 cut(s) 139, 262, 972, 1804
HpyCH4III ACNGT 9 cut(s) 279, 791, 972, 1966, 2177, 2206, 2369, 2450, 2459
HpyF10VI GCNNNNNNNGC 4 cut(s) 100, 180, 1513, 2495
HpyF3I CTNAG 9 cut(s) 303, 365, 504, 596, 1105, 1128, 1261, 1827, 2358
Hsp92I GRCGYC 1 cut(s) 225
HspAI GCGC 4 cut(s) 181, 602, 1109, 1587
KflI GGGWCCC 1 cut(s) 141
KspAI GTTAAC 1 cut(s) 580
Kzo9I GATC 9 cut(s) 778, 811, 817, 1156, 1660, 1820, 1923, 2319, 2324
LguI GCTCTTC 1 cut(s) 110
LmnI GCTCC 3 cut(s) 209, 448, 2272
Lsp1109I GCAGC 6 cut(s) 399, 783, 811, 1849, 2329, 2351
LweI GCATC 6 cut(s) 367, 1076, 1343, 1660, 1824, 2352
MabI ACCWGGT 1 cut(s) 621
MaeI CTAG 2 cut(s) 1016, 1256
MaeIII GTNAC 5 cut(s) 805, 1691, 1880, 2430, 2440
MalI GATC 9 cut(s) 780, 813, 819, 1158, 1662, 1822, 1925, 2321, 2326
MboI GATC 9 cut(s) 778, 811, 817, 1156, 1660, 1820, 1923, 2319, 2324
MflI RGATCY 3 cut(s) 811, 1820, 1923
MhlI GDGCHC 8 cut(s) 162, 512, 730, 1015, 2040, 2277, 2355, 2521
MlsI TGGCCA 1 cut(s) 1363
MluNI TGGCCA 1 cut(s) 1363
MlyI GAGTC 5 cut(s) 1097, 1129, 1556, 2188, 2316
MmeI TCCRAC 7 cut(s) 147, 192, 246, 426, 1580, 2226, 2396
Mox20I TGGCCA 1 cut(s) 1363
Mph1103I ATGCAT 1 cut(s) 1069
MroXI GAANNNNTTC 2 cut(s) 1032, 2115
MscI TGGCCA 1 cut(s) 1363
MseI TTAA 8 cut(s) 281, 579, 845, 1529, 1568, 1911, 1928, 2283
MslI CAYNNNNRTG 1 cut(s) 1278
Msp20I TGGCCA 1 cut(s) 1363
MspA1I CMGCKG 2 cut(s) 1340, 2342
MspI CCGG 4 cut(s) 11, 201, 293, 767
MspR9I CCNGG 6 cut(s) 247, 294, 513, 623, 768, 993
Mva1269I GAATGC 1 cut(s) 1397
MvaI CCWGG 4 cut(s) 247, 513, 623, 993
MvnI CGCG 1 cut(s) 1587
MwoI GCNNNNNNNGC 4 cut(s) 100, 180, 1513, 2495
NciI CCSGG 2 cut(s) 294, 768
NdeII GATC 9 cut(s) 778, 811, 817, 1156, 1660, 1820, 1923, 2319, 2324
NlaIV GGNNCC 4 cut(s) 142, 143, 205, 1460
NmuCI GTSAC 4 cut(s) 805, 1691, 1880, 2440
NsiI ATGCAT 1 cut(s) 1069
NspI RCATGY 2 cut(s) 1097, 2472
NspV TTCGAA 1 cut(s) 2265
PaeI GCATGC 1 cut(s) 2472
PagI TCATGA 1 cut(s) 1779
PciSI GCTCTTC 1 cut(s) 110
PcsI WCGNNNNNNNCGW 3 cut(s) 39, 231, 1591
PctI GAATGC 1 cut(s) 1397
PdmI GAANNNNTTC 2 cut(s) 1032, 2115
PfeI GAWTC 5 cut(s) 661, 679, 895, 1028, 2230
PflFI GACNNNGTC 1 cut(s) 41
PfoI TCCNGGA 1 cut(s) 292
PkrI GCNGC 8 cut(s) 70, 185, 389, 773, 801, 1839, 2341, 2344
PleI GAGTC 5 cut(s) 1097, 1129, 1555, 2188, 2315
PpsI GAGTC 5 cut(s) 1097, 1129, 1555, 2188, 2315
Ppu21I YACGTR 3 cut(s) 1243, 1595, 1895
PpuMI RGGWCCY 1 cut(s) 141
PsiI TTATAA 2 cut(s) 1166, 2532
Psp124BI GAGCTC 1 cut(s) 2521
Psp1406I AACGTT 1 cut(s) 1435
Psp5II RGGWCCY 1 cut(s) 141
Psp6I CCWGG 4 cut(s) 245, 511, 621, 991
PspEI GGTNACC 1 cut(s) 1691
PspFI CCCAGC 1 cut(s) 2041
PspGI CCWGG 4 cut(s) 245, 511, 621, 991
PspN4I GGNNCC 4 cut(s) 142, 143, 205, 1460
PspPI GGNCC 5 cut(s) 141, 249, 995, 2009, 2289
PspPPI RGGWCCY 1 cut(s) 141
PsrI GAACNNNNNNTAC 2 cut(s) 1942, 1974
PstNI CAGNNNCTG 2 cut(s) 91, 976
PsuI RGATCY 3 cut(s) 811, 1820, 1923
PsyI GACNNNGTC 1 cut(s) 41
PvuII CAGCTG 2 cut(s) 1340, 2342
RsaI GTAC 8 cut(s) 192, 402, 948, 1050, 1241, 1601, 1893, 2461
RsaNI GTAC 8 cut(s) 191, 401, 947, 1049, 1240, 1600, 1892, 2460
RseI CAYNNNNRTG 1 cut(s) 1278
SacI GAGCTC 1 cut(s) 2521
SapI GCTCTTC 1 cut(s) 110
SaqAI TTAA 8 cut(s) 281, 579, 845, 1529, 1568, 1911, 1928, 2283
SatI GCNGC 8 cut(s) 69, 184, 388, 772, 800, 1838, 2340, 2343
Sau3AI GATC 9 cut(s) 778, 811, 817, 1156, 1660, 1820, 1923, 2319, 2324
Sau96I GGNCC 5 cut(s) 141, 249, 995, 2009, 2289
SchI GAGTC 5 cut(s) 1097, 1129, 1556, 2188, 2316
ScrFI CCNGG 6 cut(s) 247, 294, 513, 623, 768, 993
SduI GDGCHC 8 cut(s) 162, 512, 730, 1015, 2040, 2277, 2355, 2521
SexAI ACCWGGT 1 cut(s) 621
SfaNI GCATC 6 cut(s) 367, 1076, 1343, 1660, 1824, 2352
SfcI CTRYAG 1 cut(s) 1953
SfuI TTCGAA 1 cut(s) 2265
SinI GGWCC 4 cut(s) 141, 249, 2009, 2289
SmiMI CAYNNNNRTG 1 cut(s) 1278
SmlI CTYRAG 1 cut(s) 1614
SmoI CTYRAG 1 cut(s) 1614
SnaBI TACGTA 1 cut(s) 1895
SphI GCATGC 1 cut(s) 2472
SsiI CCGC 9 cut(s) 69, 97, 138, 184, 256, 572, 708, 1205, 2074
SspI AATATT 2 cut(s) 2115, 2159
SspMI CTAG 2 cut(s) 1016, 1256
SstI GAGCTC 1 cut(s) 2521
StyD4I CCNGG 6 cut(s) 245, 292, 511, 621, 766, 991
StyI CCWWGG 1 cut(s) 1934
TaaI ACNGT 9 cut(s) 279, 791, 972, 1966, 2177, 2206, 2369, 2450, 2459
TaqI TCGA 7 cut(s) 45, 132, 156, 1486, 2218, 2265, 2378
TaqII GACCGA 1 cut(s) 2026
TatI WGTACW 4 cut(s) 946, 1048, 1599, 2459
TauI GCSGC 2 cut(s) 71, 186
TfiI GAWTC 5 cut(s) 661, 679, 895, 1028, 2230
Tru1I TTAA 8 cut(s) 281, 579, 845, 1529, 1568, 1911, 1928, 2283
Tru9I TTAA 8 cut(s) 281, 579, 845, 1529, 1568, 1911, 1928, 2283
TscAI CASTG 4 cut(s) 735, 933, 1508, 2232
TseFI GTSAC 4 cut(s) 805, 1691, 1880, 2440
TseI GCWGC 6 cut(s) 387, 771, 799, 1837, 2339, 2342
Tsp45I GTSAC 4 cut(s) 805, 1691, 1880, 2440
TspGWI ACGGA 3 cut(s) 824, 967, 2107
TspRI CASTG 4 cut(s) 735, 933, 1508, 2232
Tth111I GACNNNGTC 1 cut(s) 41
VneI GTGCAC 1 cut(s) 2351
VpaK11BI GGWCC 4 cut(s) 141, 249, 2009, 2289
XapI RAATTY 6 cut(s) 343, 955, 1020, 1288, 2249, 2525
XbaI TCTAGA 1 cut(s) 1015
XceI RCATGY 2 cut(s) 1097, 2472
XmnI GAANNNNTTC 2 cut(s) 1032, 2115
XspI CTAG 2 cut(s) 1016, 1256
ZraI GACGTC 1 cut(s) 226
Zsp2I ATGCAT 1 cut(s) 1069
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.