Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0012171

Physical Location & Seq

Genomic Coordinates Forward (+)

4861025 .. 4864809

Gene Structure 3785 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g09140.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1770 bp

ATGGTGGCCGTCAAGAAAAAAAGCGCGAAAGCCACGGAGGCAGAAGACGGCGAGATTTTCTCGGCTTCTTCCTTCTCAAGCCTCGGCCTTCACTCAAATCTATGCGACCAGCTTCAAGAGAGGTTGGGCTTCAAAGGTCCGACTTTGGTTCAGGCCCAGGCGATCCCGGTGATTCTCTCCGGCCGCCACGTGTTGGTGAACGCGGCGACGGGGACGGGGAAGACGGTGGCGTATCTGGCTCCGGTTGTGCACCACCTGAGCTTGCAGCAGCCTCGGGTTCAGCGCTCCGATGGGACGTTTGCGTTGGTACTTGTGCCGACGAGAGAGCTGTGTCTGCAGGTTTATGAGATTTTGCAGAAGCTGCTGCACCGGTTTCATTGGATTGTGCCTGGTTATGTGATGGGAGGTGAGAAGAGGGAGAAGGAGAAGGCCCGGTTGCGCAAAGGCATTTCTATTCTTGTTGCGACCCCGGGAAGGCTGTTGGATCATTTGAAGCATACGGCGTCGTTTTTGCACTCGAATTTACGGTGGATCATTTTCGATGAAGCAGATAGGATTTTGGAATTGGGATTTGGGAAGGAGATAGAGGAGATTCTGGAACTTTTGGGTTCAAGGAAACATGCCAAGGATAGTGATGCGTCGAGTAAATTTGAATTTCAGAGGCAGAATTTGCTGTTGTCTGCGACCTTGAATGAAAAGGTCAATCATCTTGCAAAAATGAGCTTGGAGAAACCTGTATTGATTGGTATGCCTGACAAGAAGACGAGGTCGATTACAAAATTTGAACCTCTAGGGTCTTCGGATTCTGACACAGAGGAGCCGCAACATTCGGTTAAGGAAACAGGCTCCACTAATGAGGATTATAAACTTCCATCGCAATTGACTCAGAGATATGTTAAAGTACCATGTAACGCACGGCTTGCTGTACTTCTTTCCATTCTAAAGCATCTTTTTGAGCGAGAAACTTCAGAAAAAATTGTGCTATTCTTTTCAACATGTGATGCAGTGGATTTTCACTACTCCTTGTTAAGTGAGTTCCAGTTCAAACCTAATTCAGGACCAGGATCAGAACTCAAGCAGAAATTTCTTACATGCAAGACCTTCAGGTTGCATGGCAATATGGAACAAGTGGATCGAAGAACCACATTCGAGACCTTCAAAACAGAAAAGTCGGCTCTTCTTTTGTCCACAGATGTGTCTGCAAGAGGTTTGGATTTCCCCAAAGTCAGATGTATCATACAGTATGATTCTCCAGGCGAGGCTACTGAATATGTGCATAGGGTTGGCAGAACGGCTCGCCTGGGTGAAAGAGGAGATTCAATATTATTTTTACAGCCAATTGAAATTGATTATTTGAAAGAGTTGGAGAAGCATAGTGTGTCTCTAGTAGAGTATCCACTCCTCAAAGTTTTGGATAGTTTCCCACTATATGGTCAGCAGCATATCAGGAAGTTTGTTTCCATTGATGCACATCCTTGGGTGCTGGCGTTGCAAAAGGCACTTGAAGCGTTTACAGATCAGCCAAATATAAAACCTCTGGCCAAGAATGCATTTTGCTCTTGGGTTCGTGCCTACACTGCCCACCGTGGTGAGCTGAAGAGAATTTTTGTAGCCAAGAAACTTCACTTGGGACATGTTGCCAAAAGCTTTGCATTAAAAGAACAACCTTCCTTGGTTGGGGGTTCGTTTCACAAGCAAGCACAGAAGAGGAAGAGGGAAGAAAGGCAGAAAGGAGGAATGAAAAAGAGGAAGTTTAACAGGAAAACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD DUF4217 Helicase_C

Protein Analysis

590

Amino Acids

66.68

Weight (kDa)

9.55

Isoelectric Point (pI)

40.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	49 - 235	6.9e-43	DEAD/DEAH box helicase
ResIII	PF04851	51 - 199	1.6e-10	Type III restriction enzyme, res subunit
Helicase_C	PF00271	312 - 435	1.5e-22	Helicase conserved C-terminal domain
CTE_SPB4	PF13959	508 - 557	3e-17	ATP-dependent rRNA helicase SPB4-like, C-terminal extension

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012171)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G40700
fragaria_vesca	FvH4_1g09140 FvH4_1g09140 FvH4_1g09140
malus_domestica	MD02G1096800.v1.1
prunus_persica	Prupe.7G194100_v2.0.a1
pyrus_communis	pycom02g07680 pycom15g19610
rosa_chinensis	RchiOBHm_Chr2g0095531
rosa_laevigata	RLG00000016576
rosa_multiflora	Rmu_co8308315.1_g000001 Rmu_co8333539.1_g000001
rosa_roxburghii	Rroxscaffold_2G00146220
rosa_rugosa	Rorug02G0052200
rosa_samantha	Rh2AG100000 Rh2BG101400 Rh2CG103600 Rh2DG100900
rosa_wichuraiana	Rw2G007590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	864
Acc16I	TGCGCA	1 cut(s)	440
Acc36I	ACCTGC	1 cut(s)	328
AccB7I	CCANNNNNTGG	2 cut(s)	193, 1430
AccII	CGCG	2 cut(s)	26, 203
AciI	CCGC	3 cut(s)	184, 203, 821
AclWI	GGATC	5 cut(s)	157, 492, 539, 1072, 1140
AcoI	YGGCCR	3 cut(s)	6, 181, 1539
AcsI	RAATTY	7 cut(s)	520, 647, 653, 667, 779, 1082, 1602
AcuI	CTGAAG	3 cut(s)	951, 1087, 1616
AcvI	CACGTG	1 cut(s)	190
AcyI	GRCGYC	1 cut(s)	503
AfaI	GTAC	3 cut(s)	309, 903, 927
AfeI	AGCGCT	1 cut(s)	284
AfiI	CCNNNNNNNGG	5 cut(s)	193, 474, 1055, 1430, 1677
AflIII	ACRYGT	3 cut(s)	189, 995, 1633
AgeI	ACCGGT	1 cut(s)	369
AjnI	CCWGG	5 cut(s)	156, 388, 1060, 1252, 1299
AjuI	GAANNNNNNNTTGG	4 cut(s)	555, 587, 1610, 1642
AleI	CACNNNNGTG	2 cut(s)	1193, 1587
AluBI	AGCT	7 cut(s)	112, 261, 328, 361, 723, 1594, 1647
AluI	AGCT	7 cut(s)	112, 261, 328, 361, 723, 1594, 1647
Alw21I	GWGCWC	1 cut(s)	252
Alw26I	GTCTC	2 cut(s)	1145, 1386
Alw44I	GTGCAC	1 cut(s)	248
AlwI	GGATC	5 cut(s)	157, 492, 539, 1072, 1140
AlwNI	CAGNNNCTG	1 cut(s)	361
Ama87I	CYCGRG	2 cut(s)	273, 469
Aor51HI	AGCGCT	1 cut(s)	284
AoxI	GGCC	6 cut(s)	6, 85, 153, 181, 429, 1539
ApaLI	GTGCAC	1 cut(s)	248
ApeKI	GCWGC	5 cut(s)	265, 268, 361, 364, 1438
ApoI	RAATTY	7 cut(s)	520, 647, 653, 667, 779, 1082, 1602
AsiGI	ACCGGT	1 cut(s)	369
AspLEI	GCGC	3 cut(s)	26, 285, 441
AspS9I	GGNCC	4 cut(s)	137, 154, 430, 1058
AsuC2I	CCSGG	4 cut(s)	167, 433, 470, 471
AsuHPI	GGTGA	5 cut(s)	181, 208, 419, 1316, 1601
AvaI	CYCGRG	2 cut(s)	273, 469
AvaII	GGWCC	2 cut(s)	137, 1058
BaeGI	GKGCMC	1 cut(s)	252
BalI	TGGCCA	1 cut(s)	1541
BbrPI	CACGTG	1 cut(s)	190
BbsI	GAAGAC	4 cut(s)	51, 227, 767, 789
Bbv12I	GWGCWC	1 cut(s)	252
BbvI	GCAGC	5 cut(s)	277, 280, 348, 351, 1450
BccI	CCATC	3 cut(s)	284, 394, 880
BceAI	ACGGC	4 cut(s)	64, 516, 932, 1308
BcgI	CGANNNNNNTGC	2 cut(s)	254, 288
BciT130I	CCWGG	5 cut(s)	158, 390, 1062, 1254, 1301
BciVI	GTATCC	1 cut(s)	1404
BcnI	CCSGG	4 cut(s)	167, 433, 470, 471
BcoDI	GTCTC	2 cut(s)	1145, 1386
BfaI	CTAG	2 cut(s)	791, 1385
BfmI	CTRYAG	1 cut(s)	335
BfoI	RGCGCY	1 cut(s)	286
BfuAI	ACCTGC	1 cut(s)	328
BfuI	GTATCC	1 cut(s)	1404
BglI	GCCNNNNNGGC	1 cut(s)	38
BisI	GCNGC	8 cut(s)	184, 204, 266, 269, 362, 365, 821, 1439
BlsI	GCNGC	8 cut(s)	185, 205, 267, 270, 363, 366, 822, 1440
Bme1390I	CCNGG	9 cut(s)	158, 167, 390, 433, 470, 471, 1062, 1254, 1301
Bme18I	GGWCC	2 cut(s)	137, 1058
BmeT110I	CYCGRG	2 cut(s)	273, 469
BmgT120I	GGNCC	4 cut(s)	137, 154, 430, 1058
BmiI	GGNNCC	3 cut(s)	240, 819, 847
BmrFI	CCNGG	9 cut(s)	158, 167, 390, 433, 470, 471, 1062, 1254, 1301
BmsI	GCATC	4 cut(s)	625, 955, 991, 1456
BpiI	GAAGAC	4 cut(s)	51, 227, 767, 789
BplI	GAGNNNNNCTC	2 cut(s)	44, 76
BpmI	CTGGAG	1 cut(s)	1236
Bpu10I	CCTNAGC	1 cut(s)	257
BpuEI	CTTGAG	2 cut(s)	61, 1058
BpuMI	CCSGG	4 cut(s)	167, 433, 470, 471
BsaAI	YACGTR	1 cut(s)	190
BsaBI	GATNNNNATC	1 cut(s)	1470
BsaHI	GRCGYC	1 cut(s)	503
BsaI	GGTCTC	1 cut(s)	1145
BsaWI	WCCGGW	2 cut(s)	241, 369
Bsc4I	CCNNNNNNNGG	5 cut(s)	193, 474, 1055, 1430, 1677
Bse118I	RCCGGY	1 cut(s)	369
Bse1I	ACTGG	1 cut(s)	1039
Bse8I	GATNNNNATC	1 cut(s)	1470
BseBI	CCWGG	5 cut(s)	158, 390, 1062, 1254, 1301
BseGI	GGATG	1 cut(s)	1471
BseJI	GATNNNNATC	1 cut(s)	1470
BseLI	CCNNNNNNNGG	5 cut(s)	193, 474, 1055, 1430, 1677
BseMII	CTCAG	2 cut(s)	248, 899
BseNI	ACTGG	1 cut(s)	1039
BseRI	GAGGAG	4 cut(s)	602, 830, 1326, 1391
BseSI	GKGCMC	1 cut(s)	252
BseX3I	CGGCCG	1 cut(s)	181
BseXI	GCAGC	5 cut(s)	277, 280, 348, 351, 1450
BsgI	GTGCAG	1 cut(s)	350
Bsh1236I	CGCG	2 cut(s)	26, 203
Bsh1285I	CGRYCG	1 cut(s)	184
BshFI	GGCC	6 cut(s)	8, 87, 155, 183, 431, 1541
BshTI	ACCGGT	1 cut(s)	369
BsiEI	CGRYCG	1 cut(s)	184
BsiHKAI	GWGCWC	1 cut(s)	252
BsiHKCI	CYCGRG	2 cut(s)	273, 469
BsiSI	CCGG	6 cut(s)	167, 180, 242, 370, 433, 470
BslFI	GGGAC	3 cut(s)	226, 307, 1644
BslI	CCNNNNNNNGG	5 cut(s)	193, 474, 1055, 1430, 1677
BsmAI	GTCTC	2 cut(s)	1145, 1386
BsmFI	GGGAC	3 cut(s)	226, 307, 1644
BsmI	GAATGC	1 cut(s)	1552
BsnI	GGCC	6 cut(s)	8, 87, 155, 183, 431, 1541
Bso31I	GGTCTC	1 cut(s)	1145
BsoBI	CYCGRG	2 cut(s)	273, 469
Bsp1286I	GDGCHC	1 cut(s)	252
Bsp143I	GATC	6 cut(s)	162, 484, 531, 1064, 1132, 1516
BspACI	CCGC	3 cut(s)	184, 203, 821
BspANI	GGCC	6 cut(s)	8, 87, 155, 183, 431, 1541
BspCNI	CTCAG	2 cut(s)	249, 898
BspFNI	CGCG	2 cut(s)	26, 203
BspLI	GGNNCC	3 cut(s)	240, 819, 847
BspMAI	CTGCAG	1 cut(s)	339
BspMI	ACCTGC	1 cut(s)	328
BspPI	GGATC	5 cut(s)	157, 492, 539, 1072, 1140
BspQI	GCTCTTC	1 cut(s)	1182
BspTNI	GGTCTC	1 cut(s)	1145
BsrFI	RCCGGY	1 cut(s)	369
BsrI	ACTGG	1 cut(s)	1039
BssAI	RCCGGY	1 cut(s)	369
BssMI	GATC	6 cut(s)	162, 484, 531, 1064, 1132, 1516
BssNI	GRCGYC	1 cut(s)	503
BssT1I	CCWWGG	3 cut(s)	624, 1475, 1671
Bst2UI	CCWGG	5 cut(s)	158, 390, 1062, 1254, 1301
Bst4CI	ACNGT	4 cut(s)	226, 528, 1242, 1586
Bst6I	CTCTTC	5 cut(s)	407, 1182, 1592, 1700, 1706
BstACI	GRCGYC	1 cut(s)	503
BstAPI	GCANNNNNTGC	3 cut(s)	361, 670, 920
BstBAI	YACGTR	1 cut(s)	190
BstC8I	GCNNGC	5 cut(s)	263, 921, 1297, 1485, 1698
BstDEI	CTNAG	2 cut(s)	257, 885
BstDSI	CCRYGG	2 cut(s)	33, 1585
BstENI	CCTNNNNNAGG	1 cut(s)	1053
BstF5I	GGATG	1 cut(s)	1471
BstFNI	CGCG	2 cut(s)	26, 203
BstH2I	RGCGCY	1 cut(s)	286
BstHHI	GCGC	3 cut(s)	26, 285, 441
BstKTI	GATC	6 cut(s)	165, 487, 534, 1067, 1135, 1519
BstMAI	GTCTC	2 cut(s)	1145, 1386
BstMBI	GATC	6 cut(s)	162, 484, 531, 1064, 1132, 1516
BstMCI	CGRYCG	1 cut(s)	184
BstNI	CCWGG	5 cut(s)	158, 390, 1062, 1254, 1301
BstNSI	RCATGY	4 cut(s)	623, 999, 1095, 1637
BstSCI	CCNGG	9 cut(s)	156, 165, 388, 431, 468, 469, 1060, 1252, 1299
BstSFI	CTRYAG	1 cut(s)	335
BstSLI	GKGCMC	1 cut(s)	252
BstUI	CGCG	2 cut(s)	26, 203
BstV1I	GCAGC	5 cut(s)	277, 280, 348, 351, 1450
BstV2I	GAAGAC	4 cut(s)	51, 227, 767, 789
BstZI	CGGCCG	1 cut(s)	181
BsuI	GTATCC	1 cut(s)	1404
BsuRI	GGCC	6 cut(s)	8, 87, 155, 183, 431, 1541
BtgI	CCRYGG	2 cut(s)	33, 1585
BtgZI	GCGATG	1 cut(s)	858
BtsCI	GGATG	1 cut(s)	1471
BtsI	GCAGTG	2 cut(s)	1011, 1575
BtsIMutI	CAGTG	2 cut(s)	1011, 1575
BveI	ACCTGC	1 cut(s)	328
Cac8I	GCNNGC	5 cut(s)	263, 921, 1297, 1485, 1698
CaiI	CAGNNNCTG	1 cut(s)	361
CfoI	GCGC	3 cut(s)	26, 285, 441
Cfr10I	RCCGGY	1 cut(s)	369
Cfr13I	GGNCC	4 cut(s)	137, 154, 430, 1058
Cfr9I	CCCGGG	1 cut(s)	469
CseI	GACGC	2 cut(s)	492, 627
Csp6I	GTAC	3 cut(s)	308, 902, 926
CspAI	ACCGGT	1 cut(s)	369
CviAII	CATG	7 cut(s)	620, 906, 996, 1092, 1112, 1634, 1767
CviQI	GTAC	3 cut(s)	308, 902, 926
DdeI	CTNAG	2 cut(s)	257, 885
DpnI	GATC	6 cut(s)	164, 486, 533, 1066, 1134, 1518
DpnII	GATC	6 cut(s)	162, 484, 531, 1064, 1132, 1516
EaeI	YGGCCR	3 cut(s)	6, 181, 1539
EagI	CGGCCG	1 cut(s)	181
Eam1104I	CTCTTC	5 cut(s)	407, 1182, 1592, 1700, 1706
EarI	CTCTTC	5 cut(s)	407, 1182, 1592, 1700, 1706
EclXI	CGGCCG	1 cut(s)	181
Eco130I	CCWWGG	3 cut(s)	624, 1475, 1671
Eco31I	GGTCTC	1 cut(s)	1145
Eco47I	GGWCC	2 cut(s)	137, 1058
Eco47III	AGCGCT	1 cut(s)	284
Eco52I	CGGCCG	1 cut(s)	181
Eco57I	CTGAAG	3 cut(s)	951, 1087, 1616
Eco72I	CACGTG	1 cut(s)	190
Eco88I	CYCGRG	2 cut(s)	273, 469
EcoNI	CCTNNNNNAGG	1 cut(s)	1053
EcoRII	CCWGG	5 cut(s)	156, 388, 1060, 1252, 1299
EcoT14I	CCWWGG	3 cut(s)	624, 1475, 1671
EcoT22I	ATGCAT	1 cut(s)	1552
ErhI	CCWWGG	3 cut(s)	624, 1475, 1671
FaeI	CATG	7 cut(s)	623, 909, 999, 1095, 1115, 1637, 1770
FaqI	GGGAC	3 cut(s)	226, 307, 1644
FatI	CATG	7 cut(s)	619, 905, 995, 1091, 1111, 1633, 1766
Fnu4HI	GCNGC	8 cut(s)	184, 204, 266, 269, 362, 365, 821, 1439
FokI	GGATG	1 cut(s)	1458
Fsp4HI	GCNGC	8 cut(s)	184, 204, 266, 269, 362, 365, 821, 1439
FspBI	CTAG	2 cut(s)	791, 1385
FspI	TGCGCA	1 cut(s)	440
GlaI	GCGC	3 cut(s)	25, 284, 440
GluI	GCNGC	8 cut(s)	184, 204, 266, 269, 362, 365, 821, 1439
GsuI	CTGGAG	1 cut(s)	1236
HaeII	RGCGCY	1 cut(s)	286
HaeIII	GGCC	6 cut(s)	8, 87, 155, 183, 431, 1541
HapII	CCGG	6 cut(s)	167, 180, 242, 370, 433, 470
HgaI	GACGC	2 cut(s)	492, 627
HhaI	GCGC	3 cut(s)	26, 285, 441
Hin1I	GRCGYC	1 cut(s)	503
Hin1II	CATG	7 cut(s)	623, 909, 999, 1095, 1115, 1637, 1770
Hin6I	GCGC	3 cut(s)	24, 283, 439
HinP1I	GCGC	3 cut(s)	24, 283, 439
HindIII	AAGCTT	1 cut(s)	1645
HinfI	GANTC	6 cut(s)	172, 592, 803, 883, 1247, 1316
HpaII	CCGG	6 cut(s)	167, 180, 242, 370, 433, 470
HphI	GGTGA	5 cut(s)	181, 208, 419, 1316, 1601
Hpy166II	GTNNAC	4 cut(s)	199, 250, 1188, 1512
Hpy188I	TCNGA	9 cut(s)	141, 289, 660, 802, 808, 888, 970, 1069, 1229
Hpy188III	TCNNGA	6 cut(s)	13, 116, 596, 1056, 1150, 1447
Hpy8I	GTNNAC	4 cut(s)	199, 250, 1188, 1512
Hpy99I	CGWCG	4 cut(s)	211, 322, 508, 643
HpyAV	CCTTC	9 cut(s)	82, 98, 415, 421, 468, 571, 1111, 1165, 1677
HpyCH4III	ACNGT	4 cut(s)	226, 528, 1242, 1586
HpyCH4IV	ACGT	2 cut(s)	189, 296
HpyF3I	CTNAG	2 cut(s)	257, 885
HpySE526I	ACGT	2 cut(s)	189, 296
Hsp92I	GRCGYC	1 cut(s)	503
Hsp92II	CATG	7 cut(s)	623, 909, 999, 1095, 1115, 1637, 1770
HspAI	GCGC	3 cut(s)	24, 283, 439
Kzo9I	GATC	6 cut(s)	162, 484, 531, 1064, 1132, 1516
LguI	GCTCTTC	1 cut(s)	1182
LmnI	GCTCC	4 cut(s)	244, 290, 817, 851
Lsp1109I	GCAGC	5 cut(s)	277, 280, 348, 351, 1450
LweI	GCATC	4 cut(s)	625, 955, 991, 1456
MaeI	CTAG	2 cut(s)	791, 1385
MaeII	ACGT	2 cut(s)	189, 296
MaeIII	GTNAC	1 cut(s)	908
MalI	GATC	6 cut(s)	164, 486, 533, 1066, 1134, 1518
MboI	GATC	6 cut(s)	162, 484, 531, 1064, 1132, 1516
MfeI	CAATTG	2 cut(s)	878, 1338
MhlI	GDGCHC	1 cut(s)	252
MlsI	TGGCCA	1 cut(s)	1541
MluNI	TGGCCA	1 cut(s)	1541
MlyI	GAGTC	1 cut(s)	877
MmeI	TCCRAC	3 cut(s)	164, 462, 1344
Mox20I	TGGCCA	1 cut(s)	1541
Mph1103I	ATGCAT	1 cut(s)	1552
MscI	TGGCCA	1 cut(s)	1541
MseI	TTAA	5 cut(s)	834, 897, 1028, 1655, 1755
MslI	CAYNNNNRTG	2 cut(s)	1193, 1587
Msp20I	TGGCCA	1 cut(s)	1541
MspI	CCGG	6 cut(s)	167, 180, 242, 370, 433, 470
MspR9I	CCNGG	9 cut(s)	158, 167, 390, 433, 470, 471, 1062, 1254, 1301
MunI	CAATTG	2 cut(s)	878, 1338
Mva1269I	GAATGC	1 cut(s)	1552
MvaI	CCWGG	5 cut(s)	158, 390, 1062, 1254, 1301
MvnI	CGCG	2 cut(s)	26, 203
NciI	CCSGG	4 cut(s)	167, 433, 470, 471
NdeII	GATC	6 cut(s)	162, 484, 531, 1064, 1132, 1516
NlaIII	CATG	7 cut(s)	623, 909, 999, 1095, 1115, 1637, 1770
NlaIV	GGNNCC	3 cut(s)	240, 819, 847
NmeAIII	GCCGAG	2 cut(s)	41, 63
NsbI	TGCGCA	1 cut(s)	440
NsiI	ATGCAT	1 cut(s)	1552
NspI	RCATGY	4 cut(s)	623, 999, 1095, 1637
OliI	CACNNNNGTG	2 cut(s)	1193, 1587
PciI	ACATGT	2 cut(s)	995, 1633
PciSI	GCTCTTC	1 cut(s)	1182
PctI	GAATGC	1 cut(s)	1552
PfeI	GAWTC	5 cut(s)	172, 592, 803, 1247, 1316
PflFI	GACNNNGTC	1 cut(s)	766
PflMI	CCANNNNNTGG	2 cut(s)	193, 1430
PinAI	ACCGGT	1 cut(s)	369
PkrI	GCNGC	8 cut(s)	185, 205, 267, 270, 363, 366, 822, 1440
PleI	GAGTC	1 cut(s)	877
PmaCI	CACGTG	1 cut(s)	190
PmlI	CACGTG	1 cut(s)	190
PpsI	GAGTC	1 cut(s)	877
Ppu21I	YACGTR	1 cut(s)	190
PscI	ACATGT	2 cut(s)	995, 1633
PsiI	TTATAA	1 cut(s)	864
Psp6I	CCWGG	5 cut(s)	156, 388, 1060, 1252, 1299
PspCI	CACGTG	1 cut(s)	190
PspGI	CCWGG	5 cut(s)	156, 388, 1060, 1252, 1299
PspN4I	GGNNCC	3 cut(s)	240, 819, 847
PspPI	GGNCC	4 cut(s)	137, 154, 430, 1058
PstI	CTGCAG	1 cut(s)	339
PstNI	CAGNNNCTG	1 cut(s)	361
PsyI	GACNNNGTC	1 cut(s)	766
RsaI	GTAC	3 cut(s)	309, 903, 927
RsaNI	GTAC	3 cut(s)	308, 902, 926
RseI	CAYNNNNRTG	2 cut(s)	1193, 1587
SapI	GCTCTTC	1 cut(s)	1182
SaqAI	TTAA	5 cut(s)	834, 897, 1028, 1655, 1755
SatI	GCNGC	8 cut(s)	184, 204, 266, 269, 362, 365, 821, 1439
Sau3AI	GATC	6 cut(s)	162, 484, 531, 1064, 1132, 1516
Sau96I	GGNCC	4 cut(s)	137, 154, 430, 1058
SchI	GAGTC	1 cut(s)	877
ScrFI	CCNGG	9 cut(s)	158, 167, 390, 433, 470, 471, 1062, 1254, 1301
SduI	GDGCHC	1 cut(s)	252
SfaNI	GCATC	4 cut(s)	625, 955, 991, 1456
SfcI	CTRYAG	1 cut(s)	335
SinI	GGWCC	2 cut(s)	137, 1058
SmaI	CCCGGG	1 cut(s)	471
SmiMI	CAYNNNNRTG	2 cut(s)	1193, 1587
SmlI	CTYRAG	2 cut(s)	76, 1073
SmoI	CTYRAG	2 cut(s)	76, 1073
SsiI	CCGC	3 cut(s)	184, 203, 821
SspI	AATATT	1 cut(s)	1323
SspMI	CTAG	2 cut(s)	791, 1385
StyD4I	CCNGG	9 cut(s)	156, 165, 388, 431, 468, 469, 1060, 1252, 1299
StyI	CCWWGG	3 cut(s)	624, 1475, 1671
TaaI	ACNGT	4 cut(s)	226, 528, 1242, 1586
TaiI	ACGT	2 cut(s)	192, 299
TaqI	TCGA	6 cut(s)	518, 540, 641, 770, 1135, 1149
TatI	WGTACW	1 cut(s)	925
TauI	GCSGC	3 cut(s)	186, 206, 823
TfiI	GAWTC	5 cut(s)	172, 592, 803, 1247, 1316
Tru1I	TTAA	5 cut(s)	834, 897, 1028, 1655, 1755
Tru9I	TTAA	5 cut(s)	834, 897, 1028, 1655, 1755
TscAI	CASTG	2 cut(s)	1011, 1582
TseI	GCWGC	5 cut(s)	265, 268, 361, 364, 1438
TspDTI	ATGAA	4 cut(s)	365, 558, 708, 1754
TspGWI	ACGGA	1 cut(s)	50
TspMI	CCCGGG	1 cut(s)	469
TspRI	CASTG	2 cut(s)	1011, 1582
Tth111I	GACNNNGTC	1 cut(s)	766
Van91I	CCANNNNNTGG	2 cut(s)	193, 1430
VneI	GTGCAC	1 cut(s)	248
VpaK11BI	GGWCC	2 cut(s)	137, 1058
XagI	CCTNNNNNAGG	1 cut(s)	1053
XapI	RAATTY	7 cut(s)	520, 647, 653, 667, 779, 1082, 1602
XceI	RCATGY	4 cut(s)	623, 999, 1095, 1637
XmaI	CCCGGG	1 cut(s)	469
XspI	CTAG	2 cut(s)	791, 1385
Zsp2I	ATGCAT	1 cut(s)	1552

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.